Phage separation device for culturing phage

Abstract

The utility model discloses a bacteriophage separating device for culturing bacteriophages, which relates to the technical field of bacteriophage culturing and separating devices and comprises a base, vertical columns fixedly mounted at four corners of the bottom of the base, a container arranged at the upper end of the base, a feeding pipe communicated and fixedly mounted at the upper end of the container, and a discharging pipe communicated and fixedly mounted at the bottom end of the container. A sealing plug is arranged at the discharge pipe, and one end of the sealing plug is in threaded connection with the interior of the discharge pipe and can block the sealing plug. According to the bacteriophage separation device for culturing the bacteriophage, the annular plate is driven to rotate by rotating the connecting piece, the annular plate is divided into a plurality of strip shapes by the strip-shaped grooves, and the annular plate can block the filter groove, so that the annular container is separated from the container, and a bacteriophage lysis solution is prevented from flowing back into the container from the filter groove. The backflow phenomenon is avoided, so that the phage can keep high purity in the separation process, and a high-quality phage sample is provided for subsequent experiments and applications.

Description

Technical Field

[0001] This utility model relates to the technical field of phage culture and separation devices, and in particular to a phage separation device for culturing phages. Background Technology

[0002] Phage isolation devices are specialized equipment used to isolate and purify bacteriophages from mixed samples. These devices are typically designed to process samples containing bacteriophages, such as wastewater, soil, or biological samples, separating the bacteriophages from other microorganisms and impurities through a series of processing steps.

[0003] For example, a phage isolation device for phage culture, disclosed in publication number CN217516948U, specifically includes a support plate, on which a sealed chamber is provided. The sealed chamber is cylindrical and open at its upper end. Multiple sealed chambers are provided. A sampling tube is placed inside each sealed chamber. A vent pipe is provided at one end of the sealed chamber and connected to an air chamber. A rotating block is coaxially fixedly mounted on a first drive gear, which is rotatably mounted on the support plate. The first drive gears between multiple rotating blocks are connected by a synchronous belt. A second drive gear is provided on the first drive gear. A rotating block that rotates around its own axis is provided at the bottom of the sealed chamber. Multiple adsorption holes are provided on the rotating block. This device is used for phage isolation from phage culture samples.

[0004] Existing phage isolation devices for phage culture suffer from reflux of phages and microbial impurities during use. This reflux can recontaminate the isolated phages, introducing other microbial impurities and affecting their purity and activity. This not only reduces the research value of phages but may also negatively impact subsequent applications. Therefore, we propose a new phage isolation device for phage culture. Utility Model Content

[0005] To address the shortcomings of existing technologies, this invention provides a phage isolation device for culturing phages, which solves the problems mentioned in the background section.

[0006] To achieve the above objectives, this utility model is implemented through the following technical solution: a phage separation device for culturing phages, comprising: a base, wherein columns are fixedly installed at the four corners of the bottom of the base, a container is provided at the upper end of the base, a feed pipe is connected to and fixedly installed at the upper end of the container, a discharge pipe is connected to and fixedly installed at the bottom end of the container, a sealing plug is provided at the discharge pipe, and one end of the sealing plug is threadedly connected to the discharge pipe and can seal the sealing plug.

[0007] The outer side of the container has several filter grooves arranged in a circular array. An annular container is provided on the outer side of the container and is fixedly connected to the container. The annular container communicates with the inner cavity of the container through several filter grooves. The lower end of the container extends into the base and is rotatably mounted on the base. An annular plate is provided inside the container. A connector is fixedly mounted on the upper end of the annular plate and is rotatably mounted on the container. The annular plate has multiple strip grooves arranged in a circular array.

[0008] As a further technical solution of this utility model, the lower end of the container is configured as a funnel shape.

[0009] As a further technical solution of this utility model, the connector has multiple limiting rods arranged in a circular array, and the lower ends of the limiting rods are inserted into the container.

[0010] As a further technical solution of this utility model, the annular container is connected to a storage mechanism, which is rotatably mounted on the base. The storage mechanism is connected to the annular container and is used to collect the separated material.

[0011] As a further technical solution of this utility model, the storage mechanism includes a storage unit, which is configured as a ring. The storage unit is fixedly connected to the ring container and is in a communicating state. The lower end of the storage unit is rotatably mounted on the base, and a drain hole is provided on the storage unit.

[0012] As a further technical solution of this utility model, the storage container is provided with a flow guiding slope, and an annular seat is rotatably installed in the base. The top of the annular seat contacts the bottom of the container, and the contact surfaces of the annular seat and the container are polished.

[0013] This invention provides a phage isolation device for culturing phages, which has the following advantages compared with the prior art:

[0014] 1. This design discloses a phage separation device for culturing phages. A rotating connector drives a ring plate to rotate, and strip grooves divide the ring plate into several strips. The ring plate can seal the filter tank, thus separating the ring container from the main container and preventing phage lysis buffer from flowing back into the main container from the filter tank. Preventing backflow ensures that the phage maintains high purity during separation, providing high-quality phage samples for subsequent experiments and applications.

[0015] 2. This design provides a phage separation device for culturing phages. The phage lysis solution in the annular container enters the receiving container through a guide slope. The guide slope is inclined to further prevent the solution in the receiving container from flowing back into the annular container. The solution in the annular container can be discharged into the receiving container in a timely manner, thereby improving the separation efficiency of the device and increasing the storage capacity of the device. This avoids the phage lysis solution from being unable to continuously pass through the filter tank due to the annular container being full. Attached Figure Description

[0016] Figure 1 A schematic diagram of a phage isolation device for culturing phages;

[0017] Figure 2 A cross-section of a phage isolation device for culturing phages. Figure 1 ;

[0018] Figure 3 A cross-section of a phage isolation device for culturing phages. Figure 2 ;

[0019] Figure 4 This is an enlarged schematic diagram of the annular plate of a bacteriophage isolation device used for culturing bacteriophages.

[0020] In the diagram: 1. Base; 2. Column; 3. Feed pipe; 4. Discharge pipe; 5. Sealing plug; 6. Filter tank; 7. Annular container; 8. Annular plate; 9. Connector; 10. Strip groove; 11. Limiting rod; 12. Collector; 13. Drain hole; 14. Guide slope; 15. Annular seat. Detailed Implementation

[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the protection scope of the present utility model.

[0022] Please see Figure 1-4This utility model provides a technical solution for a phage separation device for culturing phages: A phage separation device for culturing phages includes: a base 1, with columns 2 fixedly installed at the four corners of the bottom of the base 1, a container 16 provided at the upper end of the base 1, a feed pipe 3 connected and fixedly installed at the upper end of the container 16, a discharge pipe 4 connected and fixedly installed at the bottom end of the container 16, a sealing plug 5 provided at the discharge pipe 4, one end of the sealing plug 5 being threaded into the discharge pipe 4 and capable of sealing the sealing plug 5. The outer side of container 16 has several filter grooves 6 arranged in a circumferential array. An annular container 7 is disposed outside container 16, fitted onto and fixedly connected to container 16. The annular container 7 communicates with the inner cavity of container 16 through the filter grooves 6. The lower end of container 16 extends into base 1 and is rotatably mounted on base 1. An annular plate 8 is disposed inside container 16, with a connector 9 fixedly mounted on the upper end of the annular plate 8. The connector 9 is rotatably mounted on container 16. Multiple strip-shaped grooves 10 arranged in a circumferential array are formed on the annular plate 8. The lower end of container 16 is funnel-shaped. Multiple limiting rods 11 arranged in a circumferential array pass through the connector 9, with the lower ends of the limiting rods 11 inserted into container 16. By adding the mixed sample into container 16 through feed pipe 3, and driving container 16 to rotate at high speed, the rotation of container 16 generates centrifugal force, which separates the phage from the sample, forming a phage lysis solution. The phage lysate can be discharged from the filter tank 6 and collected in the annular container 7, while microbial impurities are retained inside the container 16 and discharged through the discharge pipe 4 by opening the sealing plug 5.

[0023] Among them, such as Figure 3 and Figure 4 As shown, a receiving mechanism is connected to the annular container 7. The receiving mechanism is rotatably mounted on the base 1 and is connected to the annular container 7 to collect the separated material. The receiving mechanism includes a receiver 12, which is annular in shape and fixedly connected to the annular container 7 in a communicating state. The lower end of the receiver 12 is rotatably mounted on the base 1, and a drain hole 13 is provided on the receiver 12. A guide slope 14 is provided inside the receiver 12, and an annular seat 15 is rotatably mounted inside the base 1. The top of the annular seat 15 contacts the bottom of the container 16, and the contact surfaces of the annular seat 15 and the container 16 are polished. After separation, the annular plate 8 is rotated by the rotating connector 9. The strip groove 10 divides the annular plate 8 into several strips. The annular plate 8 can block the filter tank 6, thereby separating the annular container 7 from the container 16 and preventing the phage lysate from flowing back into the container 16 from the filter tank 6. Avoiding reflux ensures that the phages maintain high purity during the separation process, providing high-quality phage samples for subsequent experiments and applications.

[0024] The working principle of this invention is as follows: A mixed sample is added to container 16 through feed pipe 3. Container 16 rotates at high speed, generating centrifugal force. This centrifugal force separates the phage from the sample, forming a phage lysate. The phage lysate is discharged through filter tank 6 and collected in annular container 7, while microbial impurities remain inside container 16. These impurities are discharged through discharge pipe 4 by opening the sealing plug 5. After separation, rotating connector 9 rotates annular plate 8, dividing it into several strips using groove 10. Annular plate 8 seals filter tank 6, separating annular container 7 from container 16, thus preventing phage lysate from flowing back into container 16 from filter tank 6. Preventing backflow ensures high purity of the phage during separation, providing high-quality phage samples for subsequent experiments and applications. During the separation process, the phage lysate in the annular container 7 enters the receiver 12 through the guide slope 14. The guide slope 14 is inclined to further prevent the solution in the receiver 12 from flowing back into the annular container 7. The solution in the annular container 7 can be discharged into the receiver 12 in a timely manner, thereby improving the separation efficiency of the device and increasing the storage capacity of the device, and preventing the phage lysate from being unable to continuously pass through the filter tank 6 due to the annular container 7 being full.

[0025] The above description is merely a preferred embodiment of this utility model. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of this utility model, and these improvements and modifications should also be considered within the scope of protection of this utility model. Structures, devices, and operating methods not specifically described or explained in this utility model are implemented according to conventional methods in the art, unless otherwise specified or limited.

Claims

1. A bacteriophage separation device for culturing bacteriophage, characterized by, include: The base (1) has columns (2) fixedly installed at the four corners of the bottom of the base (1). The upper end of the base (1) is provided with a container (16). The upper end of the container (16) is connected to and fixedly installed with a feed pipe (3). The bottom end of the container (16) is connected to and fixedly installed with a discharge pipe (4). A sealing plug (5) is provided at the discharge pipe (4). One end of the sealing plug (5) is threaded into the discharge pipe (4) and can seal the sealing plug (5). The container (16) has several filter grooves (6) arranged in a circular array on its outer side. An annular container (7) is provided on the outer side of the container (16). The annular container (7) is sleeved on the container (16) and fixedly connected to the container (16). The annular container (7) communicates with the inner cavity of the container (16) through several filter grooves (6). The lower end of the container (16) extends into the base (1). The lower end of the container (16) is rotatably mounted on the base (1). An annular plate (8) is provided inside the container (16). A connector (9) is fixedly installed on the upper end of the annular plate (8). The connector (9) is rotatably mounted on the container (16). The annular plate (8) has several strip grooves (10) arranged in a circular array on its surface.

2. The bacteriophage separation device for culturing bacteriophages according to claim 1, characterized by The lower end of the container (16) is configured as a funnel shape.

3. The bacteriophage separation device for culturing bacteriophages according to claim 2, characterized by The connector (9) has multiple limiting rods (11) arranged in a circular array, and the lower end of the limiting rods (11) is inserted into the container (16).

4. The bacteriophage separation device for culturing bacteriophages according to claim 3, characterized by The annular container (7) is connected to a storage mechanism, which is rotatably mounted on the base (1). The storage mechanism is connected to the annular container (7) and is used to collect the separated material.

5. The bacteriophage separation device for culturing bacteriophages according to claim 4, characterized by The storage mechanism includes a storage unit (12), which is ring-shaped. The storage unit (12) is fixedly connected to the ring container (7) and is in a communicating state. The lower end of the storage unit (12) is rotatably mounted on the base (1). The storage unit (12) is provided with a drain hole (13).

6. The bacteriophage separation device for culturing bacteriophages according to claim 5, wherein The container (12) is provided with a flow guide slope (14), and the base (1) is rotatably installed with an annular seat (15). The top of the annular seat (15) contacts the bottom of the container (16), and the contact surfaces of the annular seat (15) and the container (16) are polished.

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