Soft tissue cell separation device

Abstract

This invention provides a soft tissue cell separation device that can accommodate different grinding requirements by adjusting the spacing of the grinding rollers, and improves maintenance efficiency by allowing the filtration case to be quickly removed and cleaned. [Solution] The soft tissue cell separation device includes a grinding box that provides a grinding space, a fixed box fixedly connected to the bottom of the grinding box, a filtration component attached to the fixed box, and two grinding rollers 3 for grinding soft tissue provided inside the grinding box. The distance between the two grinding rollers can be adjusted by rotating a double-ended threaded rod 20 with a motor 13, and by adjusting the distance between the grinding rollers as needed, it is possible to respond to different grinding requirements.

Description

Technical Field

[0001] This invention relates to the technical field of soft tissue cells, and particularly to a soft tissue cell separation device.

Background Art

[0002] Soft tissue constitutes tissues that connect, support, and surround other structures of the body, mainly including muscles, fat, and synovial membranes, etc.

[0003] A separation device is an experimental device that efficiently and gently decomposes intact soft tissue into a single cell suspension or small tissue fragments.

[0004] However, the above separation device has the following problems. 1. Since the interval between the grinding rollers is fixed, it can only be applied to specific types of tissues. 2. In many cases, the filtration case is fixed with bolts, which takes time for disassembly, assembly, and maintenance, affecting the usage efficiency of the device.

[0005] In response to the above problems, a soft tissue cell separation device is proposed.

Summary of the Invention

Problems to be Solved by the Invention

[0006] The object of this invention is to provide a soft tissue cell separation device that solves the problems of low versatility of the device and the complexity of disassembly, assembly, and maintenance of the filtration case as described in the background art.

Means for Solving the Problems

[0007] To achieve the above object, this invention provides the following technical solutions. A soft tissue cell separation apparatus comprising a grinding box providing a grinding space, a fixed box fixedly connected to the bottom of the grinding box, a filtration component attached to the fixed box, two grinding rollers for grinding soft tissue provided inside the grinding box, a filtration case for filtering residue provided on the upper inside of the fixed box, and a suspension case for collecting the filtered cell suspension provided on the bottom inside of the fixed box.

[0008] Two fixed plates are fixedly connected to one side of the grinding box, movable blocks are rotatably connected to both sides of the grinding roller, and movable grooves are provided on both sides of the inside of the grinding box, with the movable grooves and the movable blocks slidingly connected to each other. A double-ended threaded rod is fixedly connected to the inside of the upper fixed plate, and two L-shaped plates are fixedly connected to both sides of the movable blocks, with the L-shaped plates and the double-ended threaded rod being screw-connected to each other. Disassembly and assembly parts for disassembling the filtration case are provided on the front side of the fixed box.

[0009] By adopting the above technical method, the control panel starts the motor, which rotates the double-ended threaded rod, causing the L-shaped plate to push the moving block and slide within the moving groove, thereby changing the distance between the two crushing rollers to accommodate the crushing requirements of different materials.

[0010] Further explanation of the above technical bill: The disassembly assembly part is equipped with a side door, the side door and the fixed box are rotatably connected to each other, a card block is fixedly connected to one side of the side door, a position block is fixedly connected to the front center of the fixed box, a positioning rod is screw-connected to the center of the position block, and the positioning rod and the card block are positioned in corresponding positions to each other. Two sliding plates are fixedly connected to the upper inside of the fixed box.

[0011] By adopting the above technical method, the positioning rod can be removed from the card block with a screw to release the lock on the side door, then the lock block can be pushed to detach it from the card groove, and the filter case can be pulled and slid along the slide plate to remove it, thereby enabling rapid disassembly of the filter case and improving maintenance efficiency.

[0012] To further explain the above technical method: A fixing rod is fixedly connected inside the lower fixing plate, and the fixing rod and the L-shaped plate are slidably connected to each other.

[0013] Further explanation of the above technical bill: A drive block is fixedly connected to one side of the crushing roller, a motor is fixedly connected to one side of the double-threaded rod, a top lid is slidably connected to the top of the crushing box, and a control panel is fixedly connected to one side of the fixed box.

[0014] To further explain the above technical bill: Slide blocks are fixedly connected to both sides of the filtration case, and the slide blocks and the slide plates are slidably connected to each other.

[0015] To further explain the above technical bill: an infusion tube is fixedly connected to one side of the filtration case, a pump is fixedly connected to one side of the infusion tube, and one side of the pump is connected to a liquid storage box via piping.

[0016] Further explanation of the above technical bill: A card groove is provided on one side of each slide plate, a spring is fixedly connected to one side of the interior of each slide block, a lock block is fixedly connected to one side of each spring, and the lock block and the card groove are positioned in corresponding positions to each other.

[0017] Further explanation of the above technical bill: A recovery hood is provided at the bottom of the filtration case, the recovery hood and the fixed box are fixedly connected to each other, a transport pipe is fixedly connected to the center of the bottom of the recovery hood, and the transport pipe and the suspension case are fixedly connected to each other. [Effects of the Invention]

[0018] Compared with the prior art, the beneficial effects of the present invention are as follows.

[0019] 1. The soft tissue cell separation device provided by the present invention first adjusts the distance between the two grinding rollers by the cooperation of the grinding roller, fixing plate, double-threaded rod, L-shaped plate, and fixing rod. The motor is started by the control panel, and the motor rotationally drives the double-threaded rod to adjust the distance between the two grinding rollers. The distance between the grinding rollers can be adjusted as needed to meet different grinding requirements.

[0020] 2. The soft tissue cell separation device provided by the present invention withdraws the positioning rod from inside the card block with a screw by the cooperation of the position block, card block, slide block, filter case, spring, positioning rod, lock block, and slide plate. Next, the lock block is pushed to withdraw it from inside the card groove, and then the filter case is pulled to slide the slide block along the slide plate to take it out. The filter case can be quickly taken out and cleaned, improving the maintenance efficiency.

Brief Description of the Drawings

[0021] [Figure 1] It is a perspective view of the overall structure of the present invention. [Figure 2] It is a rear view of the fixed box of the present invention. [Figure 3] It is an exploded view of the fixing plate of the present invention. [Figure 4] It is a structural view of the grinding roller of the present invention. [Figure 5] It is a structural view of the filter case of the present invention. [Figure 6] It is a structural view of the collection hood of the present invention. [Figure 7] It is a cross-sectional view of the filter case of the present invention.

Explanation of Reference Numerals

[0022] 1: Grinding box, 2: Fixing box, 3: Grinding roller, 4: Filtration case, 5: Suspension case, 6: Top lid, 7: Fixing plate, 8: Moving block, 9: Moving groove, 10: Drive block, 11: Control panel, 12: Liquid storage box, 13: Motor, 14: Pump, 15: Infusion pipe, 16: Card block, 17: Positioning block, 18: Positioning rod, 19: Side door, 20: Double-ended threaded rod, 21: L-shaped plate, 22: Fixing rod, 23: Slide plate, 24: Recovery hood, 25: Conveyor pipe, 26: Slide block, 27: Card groove, 28: Spring, 29: Lock block [Modes for carrying out the invention]

[0023] The following describes the technical solutions in embodiments of the present invention clearly and completely with reference to the drawings of the embodiments. Clearly, the embodiments described are only a part of, not all, embodiments of the present invention. All other embodiments that can be obtained by those skilled in the art without any creative work based on embodiments of the present invention are all within the scope of protection of the present invention.

[0024] To further understand the contents of this invention, it will be described in detail with reference to the drawings.

[0025] Referring to Figures 1 to 7, the soft tissue cell separation apparatus provided by the present invention comprises a grinding box (1) that provides a grinding space, a fixed box (2) fixedly connected to the bottom of the grinding box (1), a filtration component attached to the fixed box (2), two grinding rollers (3) for grinding soft tissue provided inside the grinding box (1), a filtration case (4) for filtering residue provided on the upper inside of the fixed box (2), and a suspension case (5) for collecting the filtered cell suspension provided on the bottom inside of the fixed box (2). A drive block (10) is fixedly connected to one side of each grinding roller (3), a motor (13) is fixedly connected to one side of a double-ended threaded rod (20), a top lid (6) is slidably connected to the top of the grinding box (1), and a control panel (11) is fixedly connected to one side of the fixed box (2). An infusion tube (15) is fixedly connected to one side of the filtration case (4), a pump (14) is fixedly connected to one side of the infusion tube (15), and one side of the pump (14) is connected to a liquid storage box (12) via piping. A recovery hood (24) is provided at the bottom of the filtration case (4), and the recovery hood (24) and the fixed box (2) are fixedly connected to each other. A transport tube (25) is fixedly connected to the center of the bottom of the recovery hood (24), and the transport tube (25) and the suspension case (5) are fixedly connected to each other.

[0026] Specifically, the grinding box (1) is made of 316L medical-grade stainless steel, the filtration case (4) has a built-in high-precision sterile nylon filter, the drive block (10) contains an independent servo motor and planetary gearbox, the motor (13) is preferably a closed-loop stepping motor, and the pump (14) is preferably a medical-grade peristaltic pump. The recovery hood (24) has a tapered funnel-shaped structure, and a solenoid valve (not shown) is connected in series to the outside of the transport pipe (25).

[0027] Referring to Figures 2 to 4, two fixed plates (7) are fixedly connected to one side of the grinding box (1), movable blocks (8) are rotatably connected to both sides of the grinding roller (3), movable grooves (9) are provided on both sides of the inside of the grinding box (1), and the movable grooves (9) and movable blocks (8) are slidably connected to each other. A double-ended threaded rod (20) is fixedly connected inside the upper fixed plate (7), and two L-shaped plates (21) are fixedly connected to both sides of the movable block (8), and the L-shaped plates (21) and double-ended threaded rod (20) are screw-connected to each other. Disassembly and assembly parts for disassembling the filtration case (4) are provided on the front side of the fixed box (2). A fixed rod (22) is fixedly connected inside the lower fixed plate (7), and the fixed rod (22) and L-shaped plates (21) are slidably connected to each other.

[0028] Specifically, the rotating shafts at both ends of the crushing roller (3) are rotatably connected to the moving block (8) by passing through the side wall of the crushing box (1), a double-ended threaded rod (20) is fixedly connected to the inside of the upper fixing plate (7) via bearings, the L-shaped plate (21) and the double-ended threaded rod (20) are screw-connected via a built-in nut slider, and a round rod-shaped fixing rod (22) is fixedly connected to the inside of the lower fixing plate (7).

[0029] Referring to Figures 2 and 5-7, the disassembled assembly part includes a side door (19), the side door (19) and the fixed box (2) are rotatably connected to each other, a card block (16) is fixedly connected to one side of the side door (19), a position block (17) is fixedly connected to the front center of the fixed box (2), a positioning rod (18) is screw-connected to the center of the position block (17), and the positioning rod (18) and the card block (16) are positioned in corresponding positions to each other. Two sliding plates (23) are fixedly connected to the upper inside of the fixed box (2), and sliding blocks (26) are fixedly connected to both sides of the filtration case (4), and the sliding blocks (26) and sliding plates (23) are slidably connected to each other. Each slide plate (23) is provided with a card groove (27) on one side, each slide block (26) has a spring (28) fixedly connected to one side of its interior, and each spring (28) has a lock block (29) fixedly connected to one side, with the lock block (29) and the card groove (27) positioned in corresponding locations.

[0030] Specifically, the side door (19) is preferably made of transparent polycarbonate, the surface of the slide block (26) is coated with Teflon, a card groove (27) is provided on one side of each slide plate (23), and the spring (28) is preferably a fatigue-resistant piano wire spring.

[0031] Operating principle: First, the distance between the two crushing rollers (3) is adjusted according to the soft tissue crushing requirement. The control panel (11) starts the motor (13), which rotates the double-ended threaded rod (20), causing the L-shaped plates (21) on both sides to move synchronously along the fixed rod (22), and further pushing the moving block (8) to slide within the moving groove (9), thereby adjusting the distance between the two crushing rollers (3).

[0032] The top cover (6) is opened and the soft tissue to be processed is placed inside, and the motor inside the drive block (10) is started. The motor inside the drive block (10) rotates the crushing roller (3) and crushes the soft tissue. At the same time, the pump (14) is started and the enzymatic hydrolysate in the liquid storage box (12) is sent to the filtration case (4) via the infusion tube (15) to perform enzymatic hydrolysis on the crushed tissue. The filtration case (4) filters out the uncrushed residue to obtain a pure cell suspension. The suspension is collected by the recovery hood (24), and when the solenoid valve of the transport tube (25) is opened, the transport tube (25) sends the suspension to the suspension case (5) for collection.

[0033] If maintenance of the filtration case (4) is required, the positioning rod (18) on the position block (17) is loosened to detach it from the card block (16), the side door (19) is opened, the lock block (29) is pushed to compress the spring (28), and the lock block (29) is released from the card groove (27). This allows the filtration case (4) to be pulled out along the slide plate (23), completing the process quickly.

[0034] In this specification, relational terms such as "1st," "2nd," etc., are used solely to distinguish one entity or operation from another, and do not necessarily require or imply that there is an actual relationship or order between these entities or operations. Furthermore, "includes," "equips," or any other variant terms are intended to cover non-exclusive inclusion, such that a process, method, object, or apparatus containing a set of elements includes not only those elements but also other elements not explicitly stated, or elements specific to that process, method, object, or apparatus.

[0035] Although embodiments of the present invention have been illustrated and described, those skilled in the art can make various changes, modifications, substitutions, and alterations to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is limited by the appended claims and equivalents.

Claims

1. A soft tissue cell separation device, The apparatus comprises a grinding box (1) that provides a grinding space, a fixed box (2) fixedly connected to the bottom of the grinding box (1), a filtration component attached to the fixed box (2), two grinding rollers (3) for grinding soft tissue provided inside the grinding box (1), a filtration case (4) for filtering residue provided on the upper inside of the fixed box (2), and a suspension case (5) for collecting the filtered cell suspension provided on the bottom inside of the fixed box (2); Two fixed plates (7) are fixedly connected to one side of the grinding box (1), movable blocks (8) are rotatably connected to both sides of the grinding roller (3), movable grooves (9) are provided on both sides of the inside of the grinding box (1), the movable grooves (9) and the movable blocks (8) are slidably connected to each other, a double-ended threaded rod (20) is fixedly connected inside the upper fixed plate (7), two L-shaped plates (21) are fixedly connected to both sides of the movable blocks (8), the L-shaped plates (21) and the double-ended threaded rod (20) are screw-connected to each other, and disassembly and assembly parts for disassembling the filtration case (4) are provided on the front side of the fixed box (2). A soft tissue cell separation device characterized by the following features.

2. The disassembled assembly part includes a side door (19), the side door (19) and the fixed box (2) are rotatably connected to each other, a card block (16) is fixedly connected to one side of the side door (19), a position block (17) is fixedly connected to the front center of the fixed box (2), a positioning rod (18) is screw-connected to the center of the position block (17), the positioning rod (18) and the card block (16) are positioned in corresponding positions to each other, and two sliding plates (23) are fixedly connected to the inner upper part of the fixed box (2). The soft tissue cell separation apparatus according to feature 1.

3. A fixing rod (22) is fixedly connected inside the lower fixing plate (7), and the fixing rod (22) and the L-shaped plate (21) are slidably connected to each other. The soft tissue cell separation apparatus according to feature 1.

4. A drive block (10) is fixedly connected to one side of each of the crushing rollers (3), a motor (13) is fixedly connected to one side of the double-threaded rod (20), a top lid (6) is slidably connected to the top of the crushing box (1), and a control panel (11) is fixedly connected to one side of the fixed box (2). The soft tissue cell separation apparatus according to feature 1.

5. Slide blocks (26) are fixedly connected to both sides of the filtration case (4), and the slide blocks (26) and the slide plate (23) are slidably connected to each other. The soft tissue cell separation apparatus according to feature 2.

6. An infusion tube (15) is fixedly connected to one side of the filtration case (4), a pump (14) is fixedly connected to one side of the infusion tube (15), and one side of the pump (14) is connected to a liquid storage box (12) via piping. The soft tissue cell separation apparatus according to feature 1.

7. Each of the slide plates (23) is provided with a card groove (27) on one side, each of the slide blocks (26) has a spring (28) fixedly connected to one side of its interior, and each of the springs (28) has a lock block (29) fixedly connected to one side, with the lock block (29) and the card groove (27) positioned in corresponding locations to each other. The soft tissue cell separation apparatus according to feature 5.

8. A recovery hood (24) is provided at the bottom of the filtration case (4), and the recovery hood (24) and the fixing box (2) are fixedly connected to each other. A transport pipe (25) is fixedly connected to the center of the bottom of the recovery hood (24), and the transport pipe (25) and the suspension case (5) are fixedly connected to each other. The soft tissue cell separation apparatus according to feature 1.

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