Attractants for the azalea mealybug and parasitoid wasps of scale insects

Abstract

To provide a composition or a compound that can attract Crisicoccus azaleae.SOLUTION: Use of a compound represented by formula (1) (where R1 and R2 each independently represent a C1-3 alkyl group) makes it possible to attract males of Crisicoccus azaleae, disrupt male-female communication of Crisicoccus azaleae, or attract parasitic wasps of scale insects.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to an attractant for male azalea mealybugs and parasitic wasps of scale insects, and more specifically, to a composition for attracting male azalea mealybugs, a composition for disrupting communication between male and female azalea mealybugs, a composition for attracting parasitic wasps of scale insects, a device for capturing male azalea mealybugs, a method for disrupting communication between male and female azalea mealybugs, and a method for attracting parasitic wasps of scale insects, as well as compounds suitable for them. [Background technology]

[0002] The azalea mealybug (Crisicoccus azaleae) is known as a pest that attacks fruit trees such as pears and persimmons, and horticultural plants such as azaleas. Insecticides are used to control them, but the overuse of insecticides has a negative impact on beneficial insects such as honeybees, so it is desirable to control them efficiently using as little as possible. To do this, it is necessary to accurately grasp the amount and timing of occurrence of the pest.

[0003] Traps using attractants such as sex pheromones are used as a means of monitoring the occurrence of pests. Sex pheromones are chemical substances secreted by female adults that are species-specific and have a strong attracting effect on male adults of the same species. Therefore, if the chemical structure of sex pheromones could be clarified and sex pheromone traps could be industrially synthesized and supplied, it would be possible to efficiently and easily investigate the occurrence of pests (Non-Patent Document 1). It is also known that sex pheromones can be used as mating disruptants (Non-Patent Document 2), and registration of pesticides is also underway. Sex pheromones often attract not only male pests, but also their natural enemies (Non-Patent Document 3). The attracted natural enemies prey on or parasitize pests that exist in the vicinity, so the attractants of the natural enemies may be used as an alternative to insecticides for pest control (Non-Patent Documents 4 and 5).

[0004] Sex pheromones of many pest species have already been identified and are used as materials, including traps, for pest management (e.g., Patent Documents 1 and 2). In addition, Non-Patent Document 6 describes a method for synthesizing various capsicumoids. However, none of the documents describes the sex pheromones or attractants of the azalea mealybug. [Prior art documents] [Patent documents]

[0005] [Patent Document 1] Patent No. 4734553 [Patent Document 2] Patent No. 5857369 [Non-patent literature]

[0006] [Non-Patent Document 1] Nobuo Sawamura et al., Prediction of emergence of larvae of the Japanese persimmon mealybug (Hemiptera: Pseudococcidae) using sex pheromones and effective accumulated temperature, Journal of the Japanese Society of Applied Entomology and Zoology, Vol. 59, No. 4, 2015, p. 183-189. https: / / doi.org / 10.1303 / jjaez.2015.183 [Non-Patent Document 2] Mayumi Teshiba et al., Mating disruption effect of sex pheromone components on the Fuji mealybug Planococcus kraunhiae (Kuwana) (Hemiptera: Pseudococcidae), Journal of the Japanese Society of Applied Entomology and Zoology, Vol. 53, No. 4, 2009, p. 173-180. https: / / doi.org / 10.1303 / jjaez.2009.173 [Non-Patent Document 3] Franco JC, Silva EB, Cortegano E et al (2008) Kairomonal response of the parasitoid Anagyrus spec. nov. near pseudococci to the sex pheromone of the vine mealybug. Entomol Exp Appl 126:122–130. https: / / doi.org / 10.1111 / j.1570-7458.2007.00643.x

Outdoor Tools 4

Direct Environment 5

Outdoor Configuration6

[0007] If the sex pheromone or attractant of the azalea mealybug is identified, it will be possible to efficiently monitor and control the azalea mealybug. Therefore, an object of the present invention is to provide a composition or compound capable of attracting the azalea mealybug. [Means for solving the problem]

[0008] As a result of intensive research aimed at solving the above problems, the present inventors have found that a compound having a specific structure has activity to attract male azalea mealybugs and parasitic wasps of mealybugs, and have completed the present invention. That is, the present invention provides the following composition, trapping device, method, and compound. [1] Formula (1): [ka] (1) (In the formula, R 1 and R 2 are each independently an alkyl group having 1 to 3 carbon atoms. A composition for attracting male azalea mealybugs and / or disrupting male-female mating of azalea mealybugs, comprising a compound represented by the formula: [2] The composition described in [1] above, further comprising another active ingredient that attracts scale insects and / or another active ingredient that disrupts male-female mating of scale insects. [3] Formula (1): [ka] (1) (In the formula, R 1 and R 2 are each independently an alkyl group having 1 to 3 carbon atoms. A composition for attracting parasitic wasps of scale insects, comprising a compound represented by the formula: [4] The composition described in [3] above, wherein the parasitic wasp includes an entocrine wasp. [5] The composition described in [3] or [4] above, further comprising another active ingredient that attracts the parasitic wasp. [6] R 1 is an ethyl group or an isopropyl group, and / or R 2 is a methyl group or an ethyl group. [7] The compound has the following chemical formula: [ka] The composition according to any one of the above items [1] to [6], having a structure represented by any one of the following: [8] Formula (1): [ka] (1) (In the formula, R 1 and R 2 are each independently an alkyl group having 1 to 3 carbon atoms. A trapping device for male azalea mealybugs containing a compound represented by the formula: [9] Formula (1): [ka] (1) (In the formula, R 1 and R 2 are each independently an alkyl group having 1 to 3 carbon atoms. A method for disrupting male-female mating of azalea mealybugs and / or for attracting parasitic wasps of scale insects, comprising the step of applying a compound represented by the formula:

[10] A compound having the following chemical formula: [ka] A compound represented by any one of the following: Effect of the Invention

[0009] According to the present invention, by using the compound represented by the above formula (1), it is possible to attract male azalea mealybugs, disrupt the communication between male and female azalea mealybugs, or attract parasitic wasps of scale insects, thereby making it possible to efficiently control azalea mealybugs. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0010] The present invention will now be described in further detail. The composition of the present invention has the formula (1): [ka] (1) (In the formula, R 1 and R 2 are each independently an alkyl group having 1 to 3 carbon atoms. The compound is a sex pheromone released by unmated females of the pest insect, an azalea mealybug, or an analog thereof.

[0011] The compound represented by the formula (1) can be synthesized by any method commonly used in the art, and may be synthesized in accordance with the method for producing the compound described in Non-Patent Document 3 (particularly, compound 3B in Scheme 1). For example, in the formula (1), R 1Alternatively, the compound represented by the formula (1) may be synthesized by preparing an unsaturated fatty acid in which R is hydrogen and subjecting it to a general dehydration condensation reaction or transesterification reaction with ethanol or isopropanol.

[0012] In one embodiment, R 1 may be an ethyl or isopropyl group, and / or R 2 may be a methyl group or an ethyl group. More specifically, the compound represented by the formula (1) is (E)-isopropyl 7-methylnon-4-enoate (compound A): [ka] (E)-Isopropyl 7-methyloct-4-enoate (Compound B): [ka] and (E)-ethyl 7-methylnon-4-enoate (compound C): [ka] In one embodiment, the composition of the present invention may simultaneously contain two or three of the compounds A to C. When the composition of the present invention contains all of the compounds A to C, the blending ratio is not particularly limited, and may be, for example, about 10 to about 8: about 5 to about 4: about 1 to about 0.5 by mass ratio.

[0013] Since the compound represented by the formula (1) functions as a sex pheromone released by female azalea mealybugs, the composition of the present invention can be used to attract male azalea mealybugs. Therefore, the composition can be used for investigating the occurrence of azalea mealybugs or for attracting and capturing (or killing) them. In addition, if the sex pheromone released by female azalea mealybugs or an analog thereof is artificially filled in their habitat or a place where they are suspected to be living, male azalea mealybugs cannot distinguish the true habitat of the female and cannot reach the female. Therefore, the composition of the present invention can also be used to disrupt communication between male and female azalea mealybugs.

[0014] The compound represented by the formula (1) also has an effect of attracting parasitic wasps, which are natural enemies of scale insects. That is, the composition of the present invention can also be used to attract the parasitic wasps. The adults of the parasitic wasps lay eggs inside the bodies of scale insects, and the larvae that hatch from the eggs grow by eating the bodies of the host scale insects. The parasitic wasps are not particularly limited, but examples thereof include cysts of the genus Anagyrus.

[0015] In one embodiment, the composition of the present invention further comprises an organic solvent. The organic solvent may be any organic solvent commonly used in the art without particular limitation, and may be, for example, a hydrocarbon organic solvent such as pentane or hexane.

[0016] In one embodiment, the composition of the present invention is in the form of a solution or emulsion. The concentration of the compound in the composition of the present invention is not particularly limited as long as the desired action is achieved, but for example, when the purpose is to investigate the occurrence of azalea mealybugs or to attract and capture (or attract and kill) them, the concentration may be about 0.001 to about 100.0 mg / mL, preferably about 0.1 to about 10 mg / mL. When the purpose is to disrupt the communication between males and females of azalea mealybugs, the concentration of the compound in the composition of the present invention may be, for example, about 0.1 to about 1000 mg / mL, preferably about 0.5 to about 500 mg / mL. When the purpose is to attract the parasitic wasps, the concentration of the compound in the composition of the present invention may be, for example, about 0.001 to about 100.0 mg / mL, preferably about 0.1 to about 50 mg / mL.

[0017] In one embodiment, the composition of the present invention is in the form of being impregnated in a sustained release carrier.The sustained release carrier can be any one that is commonly used in the art, and is not particularly limited.For example, the sustained release carrier can be a container such as a cap (septum), a thin tube, a laminated bag, or a capsule, which is made of a material having a release amount control function, such as rubber, polyethylene, polypropylene, ethylene-vinyl acetate copolymer, and polyvinyl chloride, and is preferably a septum or a thin tube made of rubber or polyethylene.

[0018] The amount of the compound impregnated per sustained release carrier is not particularly limited as long as the desired action is achieved. For example, when the purpose is to investigate the occurrence of azalea mealybugs or to attract and capture (or kill) them, the amount is about 1 cm 3The amount of the compound impregnated per sustained release carrier may be, for example, about 0.01 to about 1000 mg, preferably about 0.1 to about 1 mg. When disrupting male-female communication of azalea mealybugs, the amount of the compound impregnated per sustained release carrier may be, for example, about 1.0 to about 1000 mg, preferably about 50 to about 500 mg. When attracting parasitic wasps, the amount of the compound impregnated per sustained release carrier may be, for example, about 0.01 to about 1000 mg, preferably about 0.1 to about 5 mg.

[0019] The sustained release carrier can be used in combination with a trap for capturing pests (attached to the trap). As the trap, any trap commonly used in the art can be used without any particular limitation, and for example, the trap may be a sticky trap, a water basin trap, or a suction trap, and is preferably a sticky trap. The number of the sustained release carriers attached to the trap is not particularly limited and may be appropriately adjusted depending on the size and purpose of use of each, and for example, about 1 to about 10 sustained release carriers may be attached to the trap.

[0020] The composition of the present invention, particularly the composition impregnated in the sustained release carrier, may be installed outdoors. The installation density of the sustained release carrier outdoors is not particularly limited, but for example, when one sustained release carrier is attached to the trap for the purpose of investigating the occurrence of azalea mealybugs or attracting and capturing (or attracting and killing) them, it may be about 1 to about 1000 per 10 ares, preferably about 10 to about 500 per 10 ares. The interval between the sustained release carriers is not particularly limited, but may be, for example, about 1 to about 100 m, preferably about 5 to about 15 m. When disrupting the communication between males and females of azalea mealybugs, the installation density of the sustained release carrier outdoors may be, for example, about 50 to about 500 per 10 ares, and the interval between the sustained release carriers may be, for example, about 0.5 to 100 m, preferably about 1.4 to about 10 m. When attracting the parasitic wasps, the installation density of the controlled release carriers outdoors may be, for example, about 1 to about 1,000 carriers per 10 ares, and the distance between the controlled release carriers may be, for example, about 1 to 100 m, and is preferably about 5 to about 15 m.

[0021] The position of the composition of the present invention, particularly the composition impregnated in the sustained release carrier, is not particularly limited, but may be, for example, at a height of about 10 to about 200 cm above the ground, preferably about 30 to about 160 cm above the ground. The timing of the composition installation is not particularly limited, but may be, for example, during the adult emergence period of azalea mealybugs.

[0022] In one embodiment, the composition of the present invention is in a form impregnated in agricultural or horticultural materials such as nets and ropes. When materials impregnated with the composition of the present invention are widely installed in a field, they are particularly useful for disrupting the mating between males and females of azalea mealybugs. The method of impregnating the material is not particularly limited, and for example, the composition in a solution state may be impregnated by spraying or applying it to the material. The amount of the compound to be sprayed or applied is not particularly limited as long as the desired effect is achieved, and for example, when disrupting the mating between males and females of azalea mealybugs, the amount of the compound impregnated in the material when the material is installed in a field may be about 2.5 to about 250 g per 10 ares.

[0023] The composition of the present invention may further contain any excipients and / or additives commonly used in the art, as long as they do not impair the objectives of the present invention, and may further contain other active ingredients that attract scale insects, other active ingredients that disrupt male-female mating of scale insects, and / or other active ingredients that attract the parasitic wasps.

[0024] In another aspect, the present invention also relates to a trapping device for male azalea mealybugs, comprising the compound represented by formula (1). The trapping device can be used for investigating the occurrence of azalea mealybugs or for attracting and trapping (or attracting and killing) them. In one aspect, the trapping device of the present invention may further comprise the above-mentioned sustained release carrier and / or the above-mentioned trap. The timing of installation of the trapping device of the present invention is not particularly limited, and may be installed, for example, during the adult emergence period of azalea mealybugs.

[0025] In another aspect, the present invention also relates to a method for disrupting male-female communication of azalea mealybugs, comprising the step of applying the compound represented by formula (1) to the habitat or suspected habitat of azalea mealybugs. The present invention also relates to a method for attracting parasitic wasps of scale insects, comprising the step of applying the compound represented by formula (1) to the habitat or suspected habitat of azalea mealybugs. The method of applying the compound is not particularly limited, and for example, the compound may be diluted in a solvent capable of uniformly dispersing the compound and sprayed on the target location, or the compound may be impregnated into the sustained release carrier or the agricultural or horticultural material described above and placed on the target location.

[0026] In yet another aspect, the present invention provides (E)-isopropyl 7-methylnon-4-enoate (Compound A): [ka] (E)-Isopropyl 7-methyloct-4-enoate (Compound B): [ka] Or (E)-ethyl 7-methylnon-4-enoate (compound C): [ka] The present invention also relates to. Compounds A to C are novel compounds that can be suitably used in the above-mentioned composition, capture device, and method of the present invention. As described above, compounds A to C can be synthesized by any method commonly used in the art.

[0027] The present invention will be specifically described below with reference to examples, but the scope of the present invention is not limited to these examples. EXAMPLES

[0028] 1. Isolation of a substance that attracts male azalea mealybugs Approximately 200 virgin adult female azalea mealybugs 7 to 30 days after emergence were allowed to parasitize butternut squash and sealed in a 1.0 liter glass container. Volatile compounds, including sex attractants, released into the air were then sucked in with an air pump and captured with 1.5 g of adsorbent (Tenax TA, mesh size 20-35; GL Sciences). This was repeated for 28 days, with 4 containers per day.

[0029] The amount of sex attractant released by a total of about 22,400 females per day was collected, and the crude extract was eluted with a total of about 240 mL of hexane. When 0.1 female equivalent (1 / 10 of the amount equivalent to the amount of pheromone released by one female per day) was impregnated into a 1 cm2 filter paper and placed in a 9 cm diameter petri dish containing adult males, the activity of attracting the males was observed.

[0030] Next, the crude extract was poured into a column chromatograph packed with silica gel (0.2 g) and fractionated and eluted with 2 mL of pentane containing 0, 5, 15, or 50% diethyl ether. The attractant activity of 0.1 female equivalent of each eluted fraction was examined in the same manner as the crude extract, and the 5% diethyl ether fraction was found to have an attractive activity.

[0031] The 5% diethyl ether fraction was further fractionated using a high-performance liquid chromatograph equipped with an Inertsil SIL 100A column (particle size 5 μm, inner diameter 4.6 mm, length 250 mm; GL Science Co., Ltd.). Hexane containing 3% diethyl ether was run at a flow rate of 1 mL / min, and the attractant activity of 0.1 female equivalents of each eluted fraction was examined in the same manner as the crude extract test. The attractant activity was found in the fraction eluted from 8.5 to 10.0 minutes.

[0032] The fraction eluted from 8.5 to 10.0 minutes was further fractionated using a gas chromatograph (Agilent) equipped with a DB-1 column (inner diameter 0.25 mm, length 30 m). The temperature in the column oven was raised from an initial temperature of 60°C (held for 1 minute) to 200°C at 10°C per minute, and three components were detected: a substance eluted at 10.8 minutes (compound A), a substance eluted at 9.4 minutes (compound B), and a substance eluted at 10.3 minutes (compound C). The ratio of compounds A, B, and C (peak area ratio in the gas chromatography results) was approximately 10:5:1.

[0033] 2. Analysis of isolated compounds (1) Compound A Compound A was analyzed by a mass spectrometer (Agilent). The molecular weight was determined to be 212.17518 by high-resolution mass spectrometry, and the molecular formula was C 13 H 24 O 2 It was estimated that. The EI mass spectrum [m / z (relative intensity %)] is as follows. 41 (31.7), 43 (25.9), 55 (28.8), 57 (33.4), 67 (20.6), 68 (38.2), 69 (39.3), 70 (86.6), 71 (39.5), 81 (22.8), 83 (25.0), 84 (22.1), 95 (24.0), 96 (29.1), 97 (28.9), 109 (36.9), 113 (30.0), 114 (49.1), 123 (33.3), 135 (28.8), 141 (24.3), 152 (100), 153 (29.4), 212 (3.9)

[0034] Compound A was analyzed by a nuclear magnetic resonance spectrometer (NMR; manufactured by JEOL Ltd.). 1 H-NMR and 13 The measured values ​​of C-NMR are as follows: δ H: 5.40 (1H, ddd), 5.35 (1H, ddd), 5.03 (1H, q), 2.30 (2H, m), 2.20 (2H, t), 1.95 (1H, m), 1.77 (1H, m), 1.30 (1H, m), 1.29 (1H, m), 1.07 (1H, m), 1.04 (6H, d), 0.84 (3H, t), 0.83 (3H, d) δ C : 171.9, 130.3, 129.9, 67.2, 40.0, 35.0, 29.3, 28.4, 21.8, 19.1, 11.6

[0035] These analytical results showed that compound A had the following chemical structure: [ka]

[0036] (2) Compound B Compound B was analyzed using a mass spectrometer (Agilent). The molecular weight was determined to be 198.15836 by high-resolution mass spectrometry, and the molecular formula was C 12 H 22 O 2 It was estimated that. The EI mass spectrum [m / z (relative intensity %)] is as follows. 41 (26.4), 43 (42.1), 55 (30.9), 56 (27.8), 67 (21.2), 68 (27.2), 69 (42.3), 71 (37.8), 81 (21.6), 95 (48.1), 96 (35.7), 97 (20.1), 101 (32.1), 110 (37.9), 113 (28.1), 114 (20.1), 138 (100), 139 (45.2), 198 (1.8)

[0037] Compound B was analyzed by NMR. The measured values ​​of 1H-NMR and 13C-NMR are as follows. δ H: 5.38 (1H, dt), 5.35 (1H, dt), 5.03 (1H, sep), 2.30 (2H, dt), 2.20 (2H, t), 1.81 (2H, dd), 1.49 (1H, m), 1.04 (6H, d), 0.84 (6H, d) δ C : 171.8, 130.2, 129.6, 66.9, 42.0, 34.5, 28.4, 28.2, 22.1, 21.6

[0038] These analytical results showed that compound B had the following chemical structure: [ka]

[0039] (3) Compound C Compound C was analyzed by a mass spectrometer (Agilent). The EI mass spectrum [m / z (relative intensity %)] is as follows. 41 (30.9), 55 (31.9), 57 (29.7), 67 (29.2), 68 (41.7), 69 (38.2), 70 (38.3), 71 (33.2), 81 (27.8), 84 (44.7), 95 (28.2), 96 (29.5), 97 (25.8), 101 (21.6), 110 (24.8), 123 (33.5), 152 (100), 198 (5.3)

[0040] Compound C was analyzed by NMR. 1 H-NMR and 13 The measured values ​​of C-NMR are as follows: δ H: 5.39 (1H, ddd), 5.35 (1H, ddd), 3.96 (2H, q), 2.29 (2H, m), 2.20 (2H, t), 2.20 (2H, t), 1.95 (1H, m), 1.77 (1H, m), 1.30 (1H, m), 1.28 (1H, m), 1.07 (1H, m), 0.94 (3H, t), 0.83 (3H, t), 0.83 (3H, d);δ C : 172.4, 130.3, 129.8, 60.0, 39.9, 35.0, 34.5, 29.3, 28.3, 19.1, 14.3, 11.6.

[0041] These analytical results showed that compound C had the following chemical structure: [ka]

[0042] 3. Synthesis Examples Compound B was synthesized via the compound described in Non-Patent Document 3 (Compound 3B in Scheme 1). Specifically, 3 mL of a THF solution containing 3-methylbutanal (860 mg) was added to 11 mL of a THF solution of vinylmagnesium bromide (about 1.0 M) over 15 minutes under ice cooling, and the mixture was returned to room temperature and stirred for 16 hours. The reaction was stopped by adding 10 mL of saturated ammonium chloride water, and 3 mL of a 10% aqueous sulfuric acid solution was added, followed by extraction with diethyl ether (10 mL x 3 times). The organic layer was washed with saturated saline, dried over magnesium sulfate, and passed through a silica gel column to obtain 5-methyl-1-hexen-3-ol in a yield of 79%. This allyl alcohol (627 mg) was dissolved in orthotriethylacetate (6.29 g), and propionic acid (25 mg) was added as a catalyst, followed by heating to 138 ° C. under argon. After 3 hours, 10 mL of water was added, and the mixture was extracted with hexane (10 mL x 3 times). The mixture was dried over magnesium sulfate, concentrated, and passed through a silica gel column to obtain a product represented by formula (1) described herein, represented by R 1 is ethyl, and R 2A compound in which methyl is used (compound 3B in scheme 1 of non-patent document 3) was obtained in a yield of 83%. 110 mg of this compound was dissolved in a mixed solution of 9 mL of isopropanol and 1 mL of concentrated hydrochloric acid, and the mixture was stirred at 52°C for 16 hours, after which 10 mL of water was added and the mixture was extracted with hexane (10 mL x 3 times). The mixture was washed successively with 10 mL of saturated aqueous sodium hydrogen carbonate solution and 10 mL of saturated saline, dried over magnesium sulfate, concentrated, and passed through a silica gel column to obtain compound B in a yield of 85%. Compounds A and C were synthesized in the same manner as compound B, except that 3-methylpentanal was used instead of 3-methylbutanal.

[0043] 4. Attraction test of male adult azalea mealybugs A 0.1 mL hexane solution containing 0.1 mg of either compound A, compound B, or compound C was prepared, and this was impregnated into a rubber septum. Then, one of the rubber septa was attached to a commercially available small adhesive board (10 cm x 20 cm) as an attractant source to prepare a trapping device. In addition, taking into consideration the natural abundance ratio, a trapping device was similarly prepared using a mixture of compound A (0.1 mg), compound B (0.05 mg), and compound C (0.01 mg). Two of these trapping devices were placed outdoors and left for one week, and the number of male adult azalea mealybugs captured was counted. The results are shown in Table 1. [Table 1]

[0044] All of compounds A to C were able to attract male adult azalea mealybugs. Furthermore, when compounds A to C were mixed, a larger number of male adult azalea mealybugs were attracted. It is expected that compounds that act as sex pheromones can also be used to disrupt male-female communication.

[0045] 5. Parasitic wasp attraction test A 0.1 mL hexane solution containing a mixture of compound A (0.1 mg), compound B (0.05 mg), and compound C (0.01 mg) was prepared, and this was impregnated into a rubber septum. Then, one of the rubber septums was attached to a commercially available small adhesive board (10 cm x 20 cm) as an attractant to prepare a trapping device. For comparison, a trapping device was similarly prepared using 0.1 mg of cyclolavanduryl butyrate (CLB), a known entocrine wasp attractant (Non-Patent Document 5). These trapping devices were placed outdoors one by one and left for 4 weeks, and the captured insects were observed. As a result, entocrine wasps of the genus Anagyrus were captured not only in the trapping device using CLB but also in the trapping device using compounds A to C. The number of captured entocrine wasps was counted and is shown in Table 2.

[0046] [Table 2]

[0047] Compounds A to C were able to attract parasitic wasps in the same way as CLB. Compounds A to C are easier to synthesize than CLB, and therefore are considered to be preferable in practical use.

[0048] From the above, it was found that the compound represented by the above formula (1) has an effect of attracting male azalea mealybugs. Therefore, the compound represented by the above formula (1) can be used to capture male azalea mealybugs or to disrupt communication between male and female azalea mealybugs. It was also found that the compound represented by the above formula (1) has an effect of attracting parasitic wasps of scale insects. Therefore, by using the compound represented by the above formula (1), it is possible to efficiently control azalea mealybugs.

Claims

1. Formula (1): 【Chemistry 1】 （１） (In the formula, R 1 and R 2 are each independently an alkyl group having 1 to 3 carbon atoms. A composition for attracting male azalea mealybugs and / or disrupting male-female mating of azalea mealybugs, comprising a compound represented by the formula:

2. 2. The composition according to claim 1, further comprising another active ingredient that attracts scale insects and / or another active ingredient that disrupts male-female mating of scale insects.

3. Formula (1): 【Chemistry 2】 （１） (In the formula, R 1 and R 2 are each independently an alkyl group having 1 to 3 carbon atoms. A composition for attracting parasitic wasps of scale insects, comprising a compound represented by the formula:

4. The composition of claim 3 , wherein the parasitoid comprises an entchyster.

5. The composition according to claim 3 or 4, further comprising another active ingredient that attracts the parasitic wasp.

6. R 1 is an ethyl group or an isopropyl group, and / or R 2 The composition according to any one of claims 1 to 5, wherein is a methyl group or an ethyl group.

7. The compound has the following chemical formula: 【Chemistry 3】 The composition according to any one of claims 1 to 6, having a structure represented by any one of the following:

8. Formula (1): 【Chemistry 4】 （１） (In the formula, R 1 and R 2 are each independently an alkyl group having 1 to 3 carbon atoms. A trapping device for male azalea mealybugs containing a compound represented by the formula:

9. Formula (1): 【Chemistry 5】 （１） (In the formula, R 1 and R 2 are each independently an alkyl group having 1 to 3 carbon atoms. A method for disrupting male-female mating of azalea mealybugs and / or for attracting parasitic wasps of scale insects, comprising a step of applying a compound represented by the formula:

10. The chemical formula: 【Chemistry 6】 A compound represented by any one of the following:

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