AKT inhibitors

Abstract

The present invention discloses AKT inhibitors, specifically compounds of Formula I or pharmaceutically acceptable salts thereof. The present invention also provides methods for their preparation and use for preventing and / or treating diseases mediated by AKT protein kinase. [C173] JPEG2022517866000193.jpg4742

Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This application claims priority to Chinese patent application No. 201910084801.3, filed with the China Intellectual Property Office on January 29, 2019, the entire contents of which are hereby incorporated by reference in their entirety into this application. [Technical field]

[0002] The present invention belongs to the field of medicinal chemistry, specifically to AKT inhibitors, their preparation methods and pharmaceutical uses. [Background technology]

[0003] The PI3K / AKT / mTOR pathway, which consists of phosphatidylinositol 3-kinase (PI3K) and its downstream protein AKT (also called protein kinase, PKB) and mammalian target of rapamycin (mTOR), is a very important intracellular signal transduction pathway that exerts extremely important biological functions in processes such as cell growth, survival, proliferation, apoptosis, angiogenesis, autophagy, etc. Abnormal activation of the pathway causes a series of diseases including cancer, neurological disorders, autoimmune diseases, and hematolymphatic diseases.

[0004] AKT is a serine / threonine kinase that affects cell survival, growth, metabolism, proliferation, migration and differentiation through many downstream effectors. Over 50% of human tumors, mainly prostate, pancreatic, bladder, ovarian and breast cancers, are hyperactivated by AKT. Hyperactivation of AKT leads to tumor formation, metastasis and drug resistance.

[0005] There are three subtypes of AKT: AKT1, AKT2, and AKT3. As typical kinases, each subtype is composed of an amino-terminal PH domain (Pleckstrin homology domain), a kinase domain that binds ATP in the middle, and a regulatory domain at the carboxyl terminal. The three subtypes share about 80% of their amino acid sequences, but there is a relatively large difference in the junction region between the PH domain and the kinase domain.

[0006] Currently, the target drugs for the PI3K / AKT / mTOR signal pathway are mainly PI3K inhibitors and mTOR inhibitors, and AKT is at the core of the signal transduction pathway. Inhibiting AKT activity can avoid the severe side effects caused by inhibiting upstream PI3K, while also avoiding the negative feedback mechanism caused by inhibiting downstream mTOR from affecting the drug's effectiveness. Therefore, the search for effective and selective AKT inhibitors is currently an important direction for the development of tumor targeting drugs. CN101631778A discloses cyclopenta[D]pyrimidine derivatives, CN101578273A discloses hydroxylated and methoxylated cyclopenta[D]pyrimidine derivatives, CN101511842A discloses dihydrofuropyrimidine derivatives, and CN101970415A discloses 5H-cyclopenta[D]pyrimidine derivatives, and these compounds have AKT1 inhibition IC values ​​of less than 10 μM. 50 has. Summary of the Invention

[0007] In one embodiment, the present invention provides a compound of formula I, or a pharma- ceutically acceptable salt thereof: [ka] where: R 1 is selected from the group consisting of H, OH, halogen, CN, NH2, NO2 and C1-C6 alkyl groups optionally substituted with halogen or OH; R 2 , R 3are each independently selected from the group consisting of H, a C1-C6 alkyl group, a C3-C6 cycloalkyl group, a (C3-C6 cycloalkyl group)-(CH2)-, a (C3-C6 cycloalkyl group)-(CH2CH2)-, a benzyl group, a phenethyl group, a pyrrolidinyl group, a tetrahydrofuranyl group, and a tetrahydropyranyl group, wherein the C1-C6 alkyl group, the C3-C6 cycloalkyl group, the (C3-C6 cycloalkyl group)-(CH2)-, or the (C3-C6 cycloalkyl group)-(CH2CH2)- is the benzyl or phenethyl group is optionally substituted with halogen, OH, CN, NO, NH, C-C alkoxy, halogenated C-C alkoxy, C-C alkyl or halogenated C-C alkyl; the pyrrolidinyl, tetrahydrofuranyl or tetrahydropyranyl group is optionally substituted with halogen, OH, C-C alkyl, cyclopropylmethyl or C-C alkanoyl; Or, R 1 , R 2 and the atoms to which they are connected together form a 4- to 7-membered nitrogen-containing heterocycle; m and n are each independently selected from the group consisting of 0, 1, 2, and 3; R 4 , R 5 are all hydrogen or R 4 , R 5 together form =O, [ka] is selected from the group consisting of: [ka] R 6 , R 7 , R 8 , R 9 are each independently selected from the group consisting of H, CN, a C1-C6 alkyl group, and a C1-C6 alkoxy group, wherein the C1-C6 alkyl group or the C1-C6 alkoxy group is optionally substituted with a halogen, OH, CN, or a C1-C3 alkoxy group; R 10 is selected from the group consisting of H and a C1-C6 alkyl group, wherein said C1-C6 alkyl group is optionally substituted with halogen, OH, CN, or a C1-C3 alkoxy group; L is an optionally substituted 5-12 membered saturated heterocycle containing 1-2 nitrogens; G is any 1-5 R 11 is a 6-10 membered aryl group or a 5-10 membered heteroaryl group substituted with R 11 are independently selected from the group consisting of halogen, OH, CN, NH2, NO2, benzyloxy group, -NH(C1-C6 alkyl group), -N(C1-C6 alkyl group)2, -C(=O)NH2, -C(=O)NH(C1-C6 alkyl group), -C(=O)N(C1-C6 alkyl group)2, -SO2(C1-C6 alkyl group), C1-C6 alkyl group and C1-C6 alkoxy group, wherein the C1-C6 alkyl group or C1-C6 alkoxy group is optionally substituted with halogen; However, R 1 , R 2 and the atoms to which they are attached together form a 4- to 7-membered nitrogen-containing heterocycle, R 1 , R 2 Neither of them is H.

[0008] In some embodiments, R 1 is H, OH and C optionally substituted with halogen or OH 1- C alkyl groups, and in some exemplary embodiments, R 1 is selected from the group consisting of H, OH, Me, CF3, and CH2OH; and in some more exemplary embodiments, R 1 is selected from the group consisting of H and OH, and in some most exemplary embodiments, R 1 is H.

[0009] In some embodiments, R 2 sum R 3are each independently selected from the group consisting of H, a C1-C6 alkyl group, a C3-C6 cycloalkyl group, a (C3-C6 cycloalkyl group)-(CH2)-, a (C3-C6 cycloalkyl group)-(CH2CH2)-, a benzyl group, a phenethyl group, a pyrrolidinyl group, and a tetrahydropyranyl group, wherein the C1-C6 alkyl group, the C3-C6 cycloalkyl group, the (C3-C6 cycloalkyl group)-(CH2)-, or the (C3-C6 cycloalkyl group)-(CH2CH2)- is optionally substituted with F, Cl, Br, I, OH, CN, NH2, or a C1-C3 alkoxy group, the benzyl group or the phenethyl group is optionally substituted with F, Cl, Br, I, OH, OMe, CF3, or Me, and the pyrrolidinyl group or the tetrahydropyranyl group is optionally substituted with F, Cl, Br, I, OH, a C1-C3 alkyl group, a cyclopropylmethyl, or a C1-C4 alkanoyl group.

[0010] In some embodiments, R 2 sum R 3 are each independently selected from the group consisting of H, a methyl group, an ethyl group, an isopropyl group, an isobutyl group, a t-butyl group, a 3-pentyl group, CH2OH, CH2CH2OH, CH2CHOMe, CF3, CH2CF3, CH2CHF2, CH2CH2F, a cyclopropyl group, a cyclopentyl group, a cyclohexyl group, cyclopropyl-(CH2)-, cyclopentyl-(CH2)-, cyclohexyl-(CH2)-, cyclopropyl-(CH2CH2)-, cyclopentyl-(CH2CH2)-, benzyl group, a 4-fluorobenzyl group, a 4-chlorobenzyl group, a 4-fluorophenethyl group, a 4-chlorophenethyl group, and a tetrahydropyran-4-yl group.

[0011] In some exemplary embodiments, R 2 and R 3 are each independently selected from the group consisting of H, a methyl group, an ethyl group, an isopropyl group, a cyclopropyl group, a cyclohexyl group, cyclopropyl-(CH2)-, cyclohexyl-(CH2)-, and a tetrahydropyran-4-yl group.

[0012] In some more exemplary embodiments, R 2 and R 3 are each independently selected from the group consisting of H, an isopropyl group, and a cyclopropyl group.

[0013] In some embodiments, R 2 is H.

[0014] In some embodiments, R 3 is an isopropyl group or a cyclopropyl group.

[0015] In some embodiments, R 2 is H and R 3 is an isopropyl group or a cyclopropyl group.

[0016] In some embodiments, R 1 , R 2 and the atoms connected to them together form: [ka] Here, R 3 has the same meaning as above.

[0017] In some exemplary embodiments, R 1 , R 2 and the atoms connected to them together form: [ka] Here, R 3 has the same meaning as above.

[0018] In some embodiments, m is 0, 1, or 2; in some typical embodiments, m is 0 or 1; and in some more typical embodiments, m is 1.

[0019] In some embodiments, n is 0, 1, or 2; in some typical embodiments, n is 0 or 1; and in some more typical embodiments, n is 0.

[0020] In some embodiments, R 4 , R 5 are both hydrogen.

[0021] In some embodiments, R 4 , R 5 together form =O.

[0022] In some embodiments, [ka] is selected from the group consisting of: [ka] .

[0023] In some embodiments, [ka] is selected from the group consisting of: [ka] .

[0024] In some embodiments, [ka] is selected from the group consisting of: [ka] .

[0025] In some embodiments, [ka] but, [ka] and R 4 and R 5 together form =O.

[0026] In some embodiments, [ka] but [ka] and R 4 and R 5 together form =O.

[0027] In some embodiments, [ka] but [ka] and R 4 and R 5 together form =O.

[0028] In some embodiments, [ka] but [ka] and R 4 and R 5 together form =O.

[0029] In some embodiments, [ka] but [ka] and R 4 and R 5 are both H.

[0030] In some embodiments, [ka] but [ka] and R 4 and R 5 together form =O.

[0031] In some embodiments, R 6 , R 7 , R 8 , R 9 are each independently selected from the group consisting of H, CN, a C1-C6 alkyl group, and a C1-C6 alkoxy group, wherein the C1-C6 alkyl group or the C1-C6 alkoxy group is optionally substituted with F, Cl, Br, I, OH, CN, or OMe.

[0032] In some embodiments, R 6 , R 7 , R 8 , R 9 are each independently selected from the group consisting of H, CN, a C1-C6 alkyl group, and a C1-C6 alkoxy group, wherein the C1-C6 alkyl group or the C1-C6 alkoxy group is optionally substituted with F, CN, or OH.

[0033] In some embodiments, R 6 , R 7 , R 8 , R 9are each independently selected from the group consisting of H, CN, methyl, ethyl, propyl, isopropyl, CF3, CH2CF3, CH2CHF2, CH2CH2F, CH2CN, CH2CH2CN, CH2OH, CH2CH2OH, OMe, OEt, OCH2CH2CH3, and isopropoxy groups.

[0034] In some embodiments, R 6 is H, CN, a methyl group, an ethyl group, a propyl group, an isopropyl group, CF, CHCF, CHCHF, CHCHF, CHCN, CHCHCN, CHOH, CHCHOH, OMe, OEt, OCHCHCH, or an isopropoxy group; in some exemplary embodiments, R 6 is H, CN, a methyl group, an ethyl group, an isopropyl group, CF, CHCHOH, OMe, or OEt; in some more exemplary embodiments, R 6 is H, CN, a methyl group, CF3, or OMe, and in some further exemplary embodiments, R 6 is CN, a methyl group, CF, or OMe, and in some further exemplary embodiments, R 6 is H, CN, or a methyl group, and in some further exemplary embodiments, R 6 is H, CF, or a methyl group, and in some further exemplary embodiments, R 6 is a methyl group or CF3.

[0035] In some embodiments, R 7 is H.

[0036] In some embodiments, R 8 is H, CN, a methyl group, an ethyl group, a propyl group, an isopropyl group, CF, CHCF, CHCHF, CHCHF, CHCN, CHCHCN, CHOH, CHCHOH, OMe, OEt, OCHCHCH, or an isopropoxy group; in some exemplary embodiments, R 8is H, CN, a methyl group, an ethyl group, an isopropyl group, CF, CHCHOH, OMe, or OEt; and in some further exemplary embodiments, R 8 is H, CN, or a methyl group, and in some more exemplary embodiments, R 8 is H or CN, and in some more exemplary embodiments, R 8 is H or a methyl group, and in some further exemplary embodiments, R 8 is H.

[0037] In some embodiments, R 9 is H.

[0038] In some embodiments, R 10 is selected from the group consisting of H and a C1-C6 alkyl group, wherein the C1-C6 alkyl group is optionally substituted with halogen, OH, or CN; in some embodiments, R 10 is H, a methyl group, an ethyl group, a propyl group, an isopropyl group, CF, CHCF, CHCHF, CHCHF, CHCN, CHCHCN, CHOH, or CHCHOH; in some exemplary embodiments, R 10 is a methyl group, an ethyl group, an isopropyl group, CHCF, CHCHF, CHCN, or CHCHOH; and in some further exemplary embodiments, R 10 is a methyl group, an ethyl group, CHCN, or CHCHOH; and in some further exemplary embodiments, R 10 is methyl.

[0039] In some embodiments, L is optionally one or more R 12 is a group substituted with: [ka] . where: The wavy line indicates where L is attached to the carbonyl group, and the double wavy line indicates where L is attached to the pyrimidine. R12 is selected from the group consisting of halogen, OH, CN, vinyl, C1-C6 alkyl and C1-C6 alkoxy, wherein said C1-C6 alkyl or C1-C6 alkoxy is optionally substituted with halogen or OH; h is selected from the group consisting of 0, 1, 2, 3, and 4; j is selected from the group consisting of 0, 1, 2 and 3; k is selected from the group consisting of 1, 2, 3, and 4; q, s, v, and t are each independently selected from the group consisting of 0, 1, and 2, and none of q, s, v, and t is 0; p is selected from the group consisting of 0, 1, 2 and 3; e is selected from the group consisting of 0, 1 and 2; u is selected from the group consisting of 1, 2 and 3; W and Z are each independently selected from the group consisting of N and C, and at least one of W and Z is N.

[0040] In some embodiments, R 12 is selected from the group consisting of F, Cl, Br, I, OH, CN, vinyl, methyl, ethyl, propyl, isopropyl, CF, CHCF, CHCHF, CHCHF, OMe, OEt, CHOH, CHCHOH, OCHOH, and OCHCHOH; in some exemplary embodiments, R 12 is selected from the group consisting of methyl, ethyl, isopropyl, CF, CHCF, OMe, OEt, CHOH, and OCHOH; and in some further exemplary embodiments, R 12 is selected from the group consisting of a methyl group, an ethyl group, and CF3, and in some further exemplary embodiments, R 12 is a methyl group.

[0041] In some embodiments, h is 0, 1, 2 or 3 and j is 0, 1 or 2; in some typical embodiments, h is 0, 1 or 2 and j is 0 or 1; in some more typical embodiments, h is 0 or 1 and j is 0; and in some more typical embodiments, h is 1 and j is 0.

[0042] In some embodiments, k is 1, 2, or 3; in some typical embodiments, k is 1 or 3; and in some more typical embodiments, k is 1.

[0043] In some embodiments, q, s, v, and t are each independently 0 or 1, and q, s, v, and t are not all 0. In some exemplary embodiments, q, s, v, and t are all 1; in some more exemplary embodiments, q and s are all 0 and v and t are all 1; in some more exemplary embodiments, q and s are all 1 and v and t are all 0; and in some more exemplary embodiments, s and v are all 0 and q and t are all 1.

[0044] In some embodiments, p is 0, 1 or 2; in some typical embodiments, p is 0 or 2; and in some more typical embodiments, p is 2.

[0045] In some embodiments, e is 0 or 1 and u is 1 or 2; in some typical embodiments, e is 0 or 1 and u is 1; and in some more typical embodiments, e is 0 and u is 1.

[0046] In some embodiments, W is C and Z is N; in some typical embodiments, W is N and Z is C; and in some more typical embodiments, W and Z are both N.

[0047] In some further exemplary embodiments, L is optionally one or more of R 12 is a group substituted with: [ka] . Here, R 12 has the same meaning as above.

[0048] In some further exemplary embodiments, L is optionally one or more of R 12 is a group substituted with: [ka] . Here, R 12 has the same meaning as above.

[0049] In some further exemplary embodiments, L is selected from the group consisting of: [ka] .

[0050] In some further exemplary embodiments, L is selected from the group consisting of: [ka] .

[0051] In some further exemplary embodiments, L is selected from the group consisting of: [ka] .

[0052] In some embodiments, G is optionally selected from 1 to 5 R 11 or optionally one or more R 11 and a thienyl or pyridyl group substituted with

[0053] In some embodiments, R 11 is independently selected from the group consisting of F, Cl, Br, I, OH, CN, NH, NO, benzyloxy, methyl, ethyl, isopropyl, CHCF, CF, SMe, OMe, OCF, OEt, and isopropoxy; and in some exemplary embodiments, R 11 is independently selected from the group consisting of F, Cl, Br, CN, benzyloxy, methyl, ethyl, isopropyl, CF, OMe, SMe, and OCF; and in some further exemplary embodiments, R 11 is independently selected from the group consisting of F, Cl, and CF3, and in some further exemplary embodiments, R 11 is Cl.

[0054] In some embodiments, G is a phenyl group, a 2-chlorophenyl group, a 3-chlorophenyl group, a 4-chlorophenyl group, a 4-fluorophenyl group, a 4-bromophenyl group, a 4-methylphenyl group, a 4-ethylphenyl group, a 4-isopropylphenyl group, a 4-trifluoromethylphenyl group, a 4-cyanophenyl group, a 4-methoxyphenyl group, a 4-methylthiophenyl group, a 4-trifluoromethoxyphenyl group, a 4-chloro-3-fluorophenyl group, a 3,4-difluorophenyl group, a 2,4-dichlorophenyl group, or a 4-benzyloxyphenyl group; in some exemplary embodiments, G is a 4-chlorophenyl group, a 4-chloro-3-fluorophenyl group, a 4-trifluoromethylphenyl group, or a 3,4-difluorophenyl group; and in some further exemplary embodiments, G is a 4-chlorophenyl group.

[0055] In some embodiments, G is a thienyl or pyridyl group optionally substituted with one or more halogens; in some more typical embodiments, G is a thienyl or pyridyl group optionally substituted with one or more F, Cl, Br, or I; and in some more typical embodiments, G is the following group: [ka] .

[0056] In another aspect, the present invention provides a compound of formula II, or a pharma- ceutically acceptable salt thereof: [ka] Here, R 1 , R 2 , R 4 , R 5 , G, L, m and [ka] The definitions are the same as for compounds of formula I.

[0057] In another aspect, the present invention provides a compound of formula III, or a pharma- ceutically acceptable salt thereof: [ka] Here, R 2 , R 4 , R 5 , G, L and [ka] The definitions are the same as for compounds of formula I.

[0058] In another aspect, the present invention provides a compound of formula IV, or a pharma- ceutically acceptable salt thereof: [ka] Here, R 2 , G, L and [ka] The definitions are the same as for compounds of formula I. In another aspect, the present invention provides a compound of formula V, or a pharma- ceutically acceptable salt thereof: [ka] Here, R 2 , R 6, R 8 The definitions of , G and L are the same as for the compound of formula I.

[0059] In another aspect, the present invention provides a compound of formula VI, or a pharma- ceutically acceptable salt thereof: [ka] Here, R 2 , R 6 , R 8 , R 11 and L are defined the same as for compounds of formula I, and d is selected from the group consisting of 0, 1, 2, 3, 4, and 5.

[0060] In some embodiments, d is selected from the group consisting of 0, 1, 2, and 5; in some exemplary embodiments, d is selected from the group consisting of 0, 1, and 2; in some more exemplary embodiments, d is selected from the group consisting of 1 and 2; and in some more exemplary embodiments, d is 1. In another aspect, the present invention provides a compound of formula VII, or a pharma- ceutically acceptable salt thereof: [ka] Here, R 2 , R 6 , R 8 , R 11 and L are defined as in the compound of formula I, and d is defined as in the compound of formula VI.

[0061] In another aspect, the present invention provides a compound of formula VIII, or a pharma- ceutically acceptable salt thereof: [ka] Here, R 2 , R 8 , R 10 The definitions of , G, and L are the same as for the compound of formula I.

[0062] In another aspect, the present invention provides a compound of formula IX, or a pharma- ceutically acceptable salt thereof: [ka] Here, R 2 , R 8 , R 10 , R 11 and L are defined as in the compound of formula I, and d is defined as in the compound of formula VI. In another embodiment, the compounds of the present invention further include a compound of formula X, or a pharma- ceutically acceptable salt thereof: [ka] Here, R 1 , R 2 , R 3 , G, L, m, n and [ka] The definitions are the same as for compounds of formula I. In another aspect, the present invention further provides a compound of formula XI, or a pharma- ceutically acceptable salt thereof: [ka] Here, R 2 , R 6 The definitions of , G and L are the same as for the compound of formula I.

[0063] In another aspect, the present invention further provides a compound of formula XII, or a pharma- ceutically acceptable salt thereof: [ka] Here, R 2 , R 6 , R 11 and L are defined as in the compound of formula I, and d is defined as in the compound of formula VI. In another aspect, the present invention provides the following compound or a pharma- ceutically acceptable salt thereof: [ka] [ka] [ka] cis-(5R)-4-(5-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)hexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one, or trans-(5R)-4-(5-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)hexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one.

[0064] In another aspect, the present invention provides the following compound: [ka] .

[0065] In another aspect, the present invention further provides a pharmaceutical composition comprising a therapeutically effective amount of a compound of Formula I, II, III, IV, V, VI, VII, VIII, IX, X, XI or XII, or a pharma- ceutically acceptable salt thereof.

[0066] In some embodiments, the present invention further provides pharmaceutical compositions comprising a therapeutically effective amount of a compound of Formula I, II, III, IV, V, VI, VII, VIII, IX, X, XI or XII, or a pharma- ceutically acceptable salt thereof, and one or more pharma- ceutically acceptable carriers.

[0067] The pharmaceutical compositions of the present invention can be administered by any applicable route or method, for example, oral or parenteral (e.g., intravenous) administration. The therapeutically effective amount of a compound of formula I, II, III, IV, V, VI, VII, VIII, IX, X, XI or XII is about 0.001 mg to 20 mg / Kg body weight / day, preferably 0.01 mg to 10 mg / Kg body weight / day.

[0068] For oral administration, the pharmaceutical composition of the present invention is usually provided in the form of tablet, capsule or solution.Tablet may contain the compound of the present invention or its pharmaceutically acceptable salt, and pharmaceutically acceptable carrier.The carrier includes, but is not limited to, diluent, disintegrant, adhesive, lubricant, colorant or preservative.Capsule includes hard capsule and soft capsule.

[0069] For parenteral administration, the pharmaceutical compositions of the present invention can be administered intravenously, intramuscularly or subcutaneously. They are usually provided as a sterile aqueous solution, suspension or lyophilized powder adjusted to an appropriate pH and isotonicity.

[0070] In another aspect, the present invention further provides the use of a compound of Formula I, II, III, IV, V, VI, VII, VIII, IX, X, XI or XII in the preparation of a medicament for the prevention and / or treatment of a disease or disease state mediated by AKT protein kinase.

[0071] In another aspect, the present invention further provides a method for preventing and / or treating a disease or disease state mediated by AKT protein kinase, comprising administering to a subject in need of such treatment a compound of formula I, II, III, IV, V, VI, VII, VIII, IX, X, XI or XII of the present invention, or a pharmaceutical composition of the present invention.

[0072] In another aspect, the present invention further provides a compound of formula I, II, III, IV, V, VI, VII, VIII, IX, X, XI or XII of the present invention, or a pharmaceutical composition of the present invention, for the prevention and / or treatment of a disease or disease state mediated by AKT protein kinase.

[0073] Examples of diseases or disease states mediated by said AKT protein kinase include, but are not limited to, breast cancer, prostate cancer, or ovarian cancer.

[0074] The compound of the present invention has inhibitory effects against AKT1, AKT2 and AKT3, particularly, has a significant inhibitory effect against AKT3, has a significant inhibitory effect against cancer cell proliferation, and has good pharmacokinetic absorption and remarkably excellent oral absorption effect.

[0075] In another embodiment, the present invention provides a method for preparing a compound of formula V, including, but not limited to, the following synthetic route: Synthetic Route 1: [ka] Here, R 2 , R 6 , R 8 , G, h, and j are defined as above, and P 1 is H or an amino protecting group, P 2 is an amino protecting group, A compound of formula 1-2 is prepared from a compound of formula 1-1 in the presence of a base (e.g., sodium methoxide) and a solvent (e.g., methanol), a compound of formula 1-3 is prepared from a compound of formula 1-2 and formamidine acetate in the presence of a base (e.g., sodium methoxide) and a solvent (e.g., methanol), a compound of formula 1-4 is prepared from a compound of formula 1-3 in the presence of a base (e.g., diisopropylethylamine) and a solvent (e.g., acetonitrile), a compound of formula 1-5 is further produced from the compound of formula 1-4, a compound of formula 1-5 is prepared by reacting a compound of formula 1-6 with a compound of formula 1-7, a compound of formula 1-7 is prepared by deprotecting the protecting group, and a compound of formula 1-8 is prepared by reacting a compound of formula 1-8 with a compound of formula 1-9 to prepare a compound of formula 1-10. P 1 When is an amino protecting group, synthetic route 1 further comprises removing the amino protecting group.

[0076] Synthetic Route 2: [ka] Here, R 2 , R 6 , R 8 ,G,q,v,s,t,p are defined as above, and P 1 is H or an amino protecting group, P 2 is an amino protecting group, According to the synthetic route 1, the compound of formula 1-5 is prepared, the compound of formula 1-5 is reacted with the compound of formula 2-6 to prepare the compound of formula 2-7, the protecting group of the compound of formula 2-7 is deprotected to prepare the compound of formula 2-8, and the compound of formula 2-8 is reacted with the compound of formula 1-9 to prepare the compound of formula 2-10. P 1 When is an amino protecting group, synthetic route 2 further comprises removing the amino protecting group.

[0077] Synthetic Route 3: [ka] Here, R 2 , R 6 , R 8 , G, h, j, and k are defined as above, and P1 is H or an amino protecting group, P 2 is an amino protecting group, According to the synthetic route 1, the compound of formula 1-5 is prepared, the compound of formula 1-5 is reacted with the compound of formula 3-6 to prepare the compound of formula 3-7, the protecting group of the compound of formula 3-7 is deprotected to prepare the compound of formula 3-8, and the compound of formula 3-8 is reacted with the compound of formula 1-9 to prepare the compound of formula 3-10. P 1 When is an amino protecting group, synthetic route 3 further comprises removing the amino protecting group.

[0078] Synthetic Route 4: [ka] Here, R 2 , R 6 , R 8 , e, u are defined as above, and P 1 is H or an amino protecting group, P 2 is an amino protecting group, According to synthetic route 1, the compound of formula 1-5 is prepared, the compound of formula 1-5 is reacted with the compound of formula 4-6 to prepare the compound of formula 4-7, the protecting group of the compound of formula 4-7 is deprotected to prepare the compound of formula 4-8, and the compound of formula 4-8 is reacted with the compound of formula 1-9 to prepare the compound of formula 4-10. P 1 When is an amino protecting group, synthetic route 4 further comprises removing the amino protecting group.

[0079] In another aspect, the present invention provides a method for preparing a compound of formula VIII, including, but not limited to, the following synthetic route: Synthetic Route 5: [ka] Here, R 2 , R 10 , R 8 , G, h, and j are defined as above, and P 1 is H or an amino protecting group, P 2 is an amino protecting group, A compound of formula 5-2 is prepared from a compound of formula 5-1 in the presence of a base (e.g., sodium hydride) and a solvent (e.g., tetrahydrofuran), and the compound of formula 5-2 is reacted with NHP 2 A compound of formula 5-3 is prepared from the compound of formula 5-3 in the presence of a base (e.g., triethylamine) and a solvent (e.g., isopropanol), a compound of formula 5-4 is prepared from the compound of formula 5-3 in the presence of a base (e.g., sodium hydride) and a solvent (e.g., DMF), the protecting group of the compound of formula 5-4 is deprotected to prepare a compound of formula 5-5, the compound of formula 5-5 is reacted with the compound of formula 1-6 to prepare a compound of formula 5-7, the protecting group of the compound of formula 5-7 is deprotected to prepare a compound of formula 5-8, and the compound of formula 5-8 is reacted with the compound of formula 1-9 to prepare a compound of formula 5-10. 1 When is an amino protecting group, synthetic route 5 further comprises removing the protecting group.

[0080] Synthetic Route 6: [ka] Here, R 2 , R 10 , R 8 ,G,q,s,v,t,p are defined as above, and P 1 is H or an amino protecting group, P 2 is an amino protecting group, According to synthetic route 5, a compound of formula 5-5 is prepared, the compound of formula 5-5 is reacted with a compound of formula 2-6 to prepare a compound of formula 6-7, the protecting group of the compound of formula 6-7 is deprotected to prepare a compound of formula 6-8, and the compound of formula 6-8 is reacted with a compound of formula 1-9 to prepare a compound of formula 6-10. P 1 When is an amino protecting group, synthetic route 6 further comprises removing the protecting group.

[0081] Synthetic Route 7: [ka] Here, R 2 , R 10 , R 8 , G, h, j, and k are defined as above, and P1 is H or an amino protecting group, P 2 is an amino protecting group, According to synthetic route 5, a compound of formula 5-5 is prepared, the compound of formula 5-5 is reacted with a compound of formula 3-6 to prepare a compound of formula 7-7, the protecting group of the compound of formula 7-7 is deprotected to prepare a compound of formula 7-8, and the compound of formula 7-8 is reacted with a compound of formula 1-9 to prepare a compound of formula 7-10; P 1 When is an amino protecting group, synthetic route 7 further comprises removing the protecting group.

[0082] Synthetic Route 8: [ka] Here, R 2 , R 10 , R 8 , G, e, and u are defined as above, and P 1 is H or an amino protecting group, P 2 is an amino protecting group, According to synthetic route 5, a compound of formula 5-5 is prepared, the compound of formula 5-5 is reacted with a compound of formula 4-6 to prepare a compound of formula 8-7, the protecting group of the compound of formula 8-7 is deprotected to prepare a compound of formula 8-8, and the compound of formula 8-8 is reacted with a compound of formula 1-9 to prepare a compound of formula 8-10; P 1 When is an amino protecting group, synthetic route 8 further comprises removing the protecting group.

[0083] Synthetic Route 9: [ka] where X is a halogen and R 6 , h, j, and G are defined as above, and P 1 is H or an amino protecting group, P 2 is an amino protecting group. A compound of formula 9-2 is prepared from a compound of formula 9-1, a compound of formula 9-2 is reacted with a compound of formula 1-6 to prepare a compound of formula 9-3, the compound of formula 9-3 is reduced to produce a compound of formula 9-4, the compound of formula 9-4 is subjected to a ring-forming reaction to prepare a compound of formula 9-5, the protecting group of the compound of formula 9-5 is deprotected to produce a compound of formula 9-6 or a salt thereof, which is further reacted with a compound of formula 1-9 to prepare a compound of formula 9-7. P 1 When is an amino protecting group, synthetic route 9 further comprises removing the protecting group.

[0084] Synthetic Route 10: [ka] where X is a halogen and R 6 , q, v, s, t, p, and G are defined as above, and P 1 is H or an amino protecting group, P 2 is an amino protecting group.

[0085] A compound of formula 9-2 is prepared from a compound of formula 9-1, a compound of formula 9-2 is reacted with a compound of formula 2-6 to prepare a compound of formula 10-3, the compound of formula 10-3 is reduced to produce a compound of formula 10-4, the compound of formula 10-4 is subjected to a ring-forming reaction to prepare a compound of formula 10-5, the protecting group of the compound of formula 10-5 is deprotected to produce a compound of formula 10-6 or a salt thereof, which is further reacted with a compound of formula 1-9 to prepare a compound of formula 10-7. P 1 When is an amino protecting group, synthetic route 10 further comprises removing the protecting group.

[0086] Synthetic Route 11: [ka] where X is a halogen and R 6 , h, k, j, and G are defined as above, and P 1 is H or an amino protecting group, P 2 is an amino protecting group. A compound of formula 9-2 is prepared from a compound of formula 9-1, a compound of formula 9-2 is reacted with a compound of formula 11-1 to prepare a compound of formula 11-2, the compound of formula 11-2 is reduced to produce a compound of formula 11-3, the compound of formula 11-3 is subjected to a ring-forming reaction to prepare a compound of formula 11-4, the protecting group of the compound of formula 11-4 is deprotected to produce a compound of formula 11-5 or a salt thereof, which is further reacted with a compound of formula 1-9 to prepare a compound of formula 11-6. P 1 When is an amino protecting group, synthetic route 11 further comprises removing the protecting group.

[0087] Synthetic Route 12: [ka] Here, R 6 , R 8 , h, k, j, and G are defined as above, and P 1 is H or an amino protecting group, P 2 is an amino protecting group.

[0088] The compound of formula 1-5 is reacted with the compound of formula 11-1 to prepare the compound of formula 12-1, the protecting group of the compound of formula 12-1 is deprotected to prepare the compound of formula 12-2, and the compound of formula 12-2 is reacted with the compound of formula 1-9 to prepare the compound of formula 12-3. P 1 When is an amino protecting group, synthetic route 12 further comprises removing the protecting group.

[0089] Those skilled in the art will recognize that L is R 12 It will be appreciated that when substituted with, the compounds of the present invention may be prepared with reference to the synthetic routes described above. [Brief description of the drawings]

[0090] [Figure 1] FIG. 1 is a schematic diagram of a single molecule of Isomer 2 of Example 15. [Diagram 2] FIG. 2 is a schematic diagram of the asymmetric structural unit of the single crystal of Isomer 2 of Example 15. [Diagram 3] FIG. 3 is a schematic diagram of a single molecule of Isomer 1 of Example 34. [Figure 4] FIG. 4 is a schematic diagram of the asymmetric structural unit of the single crystal of Isomer 1 of Example 34. [Diagram 5] FIG. 5 is a schematic diagram of a single molecule of Isomer 3 of Example 34. [Figure 6] FIG. 6 is a schematic diagram of the asymmetric structural unit of the single crystal of Isomer 3 of Example 34. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0091] Related Definitions Unless otherwise stated, the following terms used in the specification and claims have the following meanings: The "compounds" described in this invention include all stereoisomers and tautomers thereof.

[0092] In this application, the compounds may be asymmetric, e.g., have one or more stereoisomers. Unless otherwise specified, all stereoisomers include, e.g., enantiomers and diastereomers. Compounds of the present invention having asymmetric carbon atoms can be isolated in optically pure or racemic forms. Optically pure forms can be separated from racemic mixtures or synthesized with chiral starting materials or chiral reagents. All racemates, diastereomers, and enantiomers are within the scope of the present invention.

[0093] The compounds of the present invention further include tautomeric forms, which result from the swapping of one single bond with the adjacent double bond and the associated migration of one proton.

[0094] The terms "optionally" or "optionally" refer to the fact that the subsequently stated event or circumstance may or may not occur, and this language includes cases where the event or circumstance occurs and cases where the event or circumstance does not occur.

[0095] Numerical ranges herein refer to each integer within the specified range, for example, "C1-C6" means that the group may have 1, 2, 3, 4, 5, or 6 carbon atoms, and "C3-C6" means that the group may have 3, 4, 5, or 6 carbon atoms.

[0096] The term "substituted" refers to any one or more hydrogens in a particular atom or group being replaced with a substituent, provided that the valence of the particular atom or group is normal and the resulting compound is stable. When the substituent is a ketone group (i.e., =O), this means that two hydrogens are replaced. Unless otherwise specified, the type and number of the substituents may be any that are chemically feasible.

[0097] When any variable (e.g., R) occurs more than one time in a composition or structure of a compound, its definition at each occurrence is independent. Thus, for example, if a group is substituted with 1-5 R, then that group may optionally be substituted with up to 5 R, and R at each occurrence has independent options. Also, combinations of substituents and / or variables are permissible only if such combinations result in stable compounds.

[0098] The term "alkyl group" refers to a saturated aliphatic hydrocarbon group, including linear or branched saturated hydrocarbon groups, the hydrocarbon group having the indicated number of carbon atoms. For example, the term "C1-C6 alkyl group" includes C1 alkyl group, C2 alkyl group, C3 alkyl group, C4 alkyl group, C5 alkyl group, C6 alkyl group, examples of which include, but are not limited to, methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, t-butyl, n-pentyl, 2-pentyl, 3-pentyl, n-hexyl, 2-hexyl, 3-hexyl, etc. They may be divalent, such as methylene, ethylene.

[0099] The term "alkoxy group" refers to a group having the structure alkyl-O-, where alkyl includes straight-chain or branched saturated monovalent hydrocarbon groups. For example, a "C1-C3 alkoxy group" includes methoxy, ethoxy, n-propoxy, and isopropoxy groups.

[0100] The term "alkanoyl group" refers to a group having the structure RC(=O)-, where R is H or a saturated aliphatic hydrocarbon group, including straight-chain or branched-chain saturated monovalent hydrocarbon groups. For example, a "C1-C4 alkanoyl group" includes a C1 alkanoyl group, a C2 alkanoyl group, a C3 alkanoyl group, and a C4 alkanoyl group, with suitable alkanoyl groups including formyl, acetyl, n-propionyl, isopropionyl, n-butyryl, isobutyryl, and t-butyryl.

[0101] The term "halogenated" refers to substitution with one or more halogen atoms, examples of which include fluorine, chlorine, bromine, and iodine. For example, the term "halogenated C1-C3 alkoxy group" refers to a C1-C3 alkoxy group substituted with one or more halogen atoms, examples of which include, but are not limited to, OCF3, OCHF2, OCH2F, OCH2CF3, OCH2CHF2, or OCF2CF3. For example, the term "halogenated C1-C3 alkyl group" refers to a C1-C3 alkyl group substituted with one or more halogen atoms, examples of which include, but are not limited to, CF3, CHF2, CH2F, CH2CF3, CH2CHF2, or CF2CF3.

[0102] The term "cycloalkyl" refers to a monocyclic saturated hydrocarbon system with no heteroatoms and no double bonds. Examples of the term "C3-C6 cycloalkyl" include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

[0103] The term "aryl group" refers to a monocyclic or fused polycyclic aromatic ring group consisting of all carbon having a conjugated pi-electron system and derived by removal of a hydrogen atom from a carbon atom of a parent aromatic ring system. For example, an aryl group may have 6-20 carbon atoms, 6-14 carbon atoms, or 6-10 carbon atoms. Bicyclic groups containing an aryl ring fused to a saturated, partially unsaturated ring or aromatic carbocyclic ring are included. Examples include, but are not limited to, phenyl, naphthyl, anthracenyl, indene, indane, 1,2-dihydronaphthalene, and 1,2,3,4-tetrahydronaphthalene.

[0104] The term "heteroaryl" refers to a 5-, 6-, or 7-membered monovalent aryl group containing at least one heteroatom independently selected from the group consisting of nitrogen, oxygen, and sulfur, and also includes fused ring systems of 5-10 atoms, at least one of which is aromatic. Illustrative examples of heteroaryl groups include, but are not limited to, pyridyl, thienyl, imidazolyl, pyrimidinyl, pyridyl, furyl, pyrazinyl, thiazolyl, quinolinyl, isoquinolinyl, indolyl, benzimidazolyl, imidazopyridyl, benzofuryl, pyridazinyl, and isoindolyl.

[0105] The term "membered" refers to the number of skeletal atoms that make up the ring. For example, "5-10 membered" refers to 5, 6, 7, 8, 9, or 10 skeletal atoms that make up the ring. Thus, by way of example, pyridine, piperidine, piperazine, and benzene are six-membered rings, and thiophene and pyrrole are five-membered rings.

[0106] The term "heterocycle" refers to a 5-12 membered saturated non-aromatic system having carbon atoms and one to two heteroatoms in the ring, where the heteroatoms are independently selected from the group consisting of nitrogen, sulfur and oxygen atoms. In heterocycle groups containing one or more nitrogen atoms, the point of attachment may be a carbon atom or a nitrogen atom, where valence allows. A heterocycle may be monocyclic or polycyclic, e.g. bicyclic, where two or more rings are present as fused, bridged or spiro rings, in which at least one ring contains one or more heteroatoms.

[0107] Where valence permits, the substituent R 12 may be attached to any atom on the ring. Combinations of substituents and / or variables are permissible only if such combinations result in stable compounds. Those of ordinary skill in the art will recognize that one or more R 12 It is understood that any group that contains substituents is not intended to introduce substitutions or modes of substitution that cannot exist in space and / or cannot be synthesized.

[0108] Wavy line [ka] and double wavy line [ka] Both refer to the bonding position of a chemical bond and have the same chemical meaning, unless otherwise specified. [ka] and [ka] The difference between them merely distinguishes the position or order of attachment.

[0109] The term "amino-protecting group" refers to a suitable protecting group to prevent side reactions on the nitrogen of an amino group. Examples of amino-protecting groups include, but are not limited to, Boc, DMB, benzyloxycarbonyl, 9-fluorenylmethoxycarbonyl, benzyl, formyl, or acetyl.

[0110] The term "pharmacologically acceptable salts" refers to salts that retain the biological effectiveness of the free acids and bases of a particular compound and that do not have any adverse biological effects, such as acid (including organic and inorganic acids) or base (including organic and inorganic bases) addition salts.

[0111] The pharma- ceutically acceptable salts of the present invention can be synthesized from parent compounds containing acids or bases by conventional chemical methods. In general, such salts are prepared by reacting these compounds in the form of a free acid or base with a stoichiometrically appropriate base or acid in water, an organic solvent, or a mixture of both.

[0112] The term "effective amount" or "therapeutically effective amount" refers to a non-toxic but sufficient dose of a pharmaceutical or drug to achieve the desired effect.

[0113] The term "pharmaceutical acceptable carrier" refers to a carrier that is not significantly irritating to the living body and does not impair the biological activity and performance of the active compound. Carriers include, but are not limited to, any diluent, disintegrant, adhesive, flow agent, or wetting agent approved by the China Food and Drug Administration for use in humans or animals.

[0114] The following abbreviations are used in the claims and specification: M:mol / L mM:mmol / L nM:nmol / L Boc: t-butoxycarbonyl DMB: 2,4-dimethoxybenzyl NMP: N-methylpyrrolidone DMAP: p-Dimethylaminopyridine DMF: N,N-dimethylformamide DEA: Diethylamine PE: Petroleum ether EA: Ethyl acetate HATU: (2-(7-azabenzotriazole)-N,N,N',N'-tetramethyluronium hexafluorophosphate) RT: retention time SFC: Supercritical Fluid Chromatography h: time min:minutes TK: tyrosine kinase SEB: Fluorescent signal enhancer HTRF: Homogeneous time-resolved fluorescence DTT: Dithiothreitol NR: Not calculated Preparation method: The preparation methods of the present invention are described in more detail below, but the scope of the present invention is not limited to these specific preparation methods. In addition, reaction conditions such as reactants, solvents, bases, amounts of compounds used, reaction temperatures, and reaction times are not limited to the following examples.

[0115] The compounds of the present invention may be prepared by combining various synthetic methods, optionally as described herein or as known in the art, and such combinations can be readily performed by one of ordinary skill in the art.

[0116] Scheme A: [ka] Reaction conditions: a) ethyl crotonate, sodium methoxide in methanol (30% wt), methanol, b) formamidine acetate, sodium methoxide in methanol (30% wt), c) phosphoryl chloride, diisopropylethylamine, acetonitrile, d) aqueous ammonia (25-28% wt), e) t-butyl piperazine-1-carboxylate, f) trifluoroacetic acid, dichloromethane, g) (S)-2-(4-chlorophenyl)-3-(isopropylamino)propionic acid, 2-(7-benzotriazole oxide)-N,N,N',N'-tetramethyluronium hexafluorophosphate, diisopropylethylamine, dichloromethane.

[0117] Example 1 4-(4-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)piperazin-1-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one [ka] a) Trimethyl 2-methylpropane-1,1,3-tricarboxylate Under a nitrogen gas atmosphere, a sodium methoxide methanol solution (30%wt) (8.16g) was added to methanol (400mL) at 20°C, and then the temperature was raised to 70°C. Dimethyl malonate (24.64g) and ethyl crotonate (21.08g) were mixed uniformly and added dropwise to the sodium methoxide methanol solution, and reacted at 70°C for 3 hours. After the reaction was completed, the solvent was removed by vacuum distillation, ethyl acetate (100mL) was added, and the pH was adjusted to 7 with 4M hydrochloric acid. Then, 100mL of water was added, and the liquid was separated. The organic phase was concentrated to obtain 45.48g of a yellow liquid, which was used in the next step without purification. 1 H NMR(400MHz,DMSO-d6)δ(ppm)0.93(d,J=6.8Hz,3H),2.26(q,J=12.0Hz,2H),2. 52-2.58(m,1H),3.56(d,J=6.8Hz,1H),3.59(s,3H),3.65(s,3H),3.67(s,3H).

[0118] b) Methyl 3-(4,6-dihydroxypyrimidin-5-yl)butyrate Under nitrogen gas atmosphere, sodium methoxide methanol solution (30%wt) (97.55g) was added to methanol (400mL) at 20℃, then cooled to -15℃, formamidine acetate (22.98g) was added and reacted for 30min, then 2-methylpropan-1,1,3-trimethyl benzoate (45.72g) was added dropwise, the temperature was gradually raised to 20℃, and the reaction was continued for 12 hours. After the reaction was completed, the reaction solution was cooled to 0℃, 4M hydrochloric acid was added to adjust the pH to 2, the solvent was removed by distillation under reduced pressure, and 100mL of water was added at 0℃, a solid precipitated, the solid was collected by suction filtration, the filter cake was washed with water (50mL), and then vacuum dried to obtain 29.60g of a yellow solid, which was used in the next step without purification. 1 H NMR (400MHz, DMSO-d6) δ (ppm) 11.62 (s, 2H), 7.86 (s, 1H), 3.53 (s, 3H), 3.34-3.42 (m, 1H), 2.58-2.70 (m, 2H), 1.11 (d, J = 6.8Hz, 3H).

[0119] c) Methyl 3-(4,6-dichloropyrimidin-5-yl)butyrate Under a nitrogen gas atmosphere, 3-(4,6-dihydroxypyrimidin-5-yl)methyl butyrate (9.1 g) was dispersed in acetonitrile (100 mL) at 22 ° C., phosphoryl chloride (16.03 g) and diisopropylethylamine (7.79 g) were added dropwise in this order, the reaction system was heated violently, the solid gradually dissolved completely, and the temperature was raised to 60 ° C. and reacted for 18 hours. After the reaction was completed, the reaction liquid was cooled to 0 ° C., poured into 200 mL of ice water, adjusted to pH 7-8 with saturated sodium bicarbonate water, extracted with ethyl acetate (50 mL × 3), the organic phase was combined, the solvent was removed by distillation under reduced pressure, and the mixture was isolated by silica gel column chromatography (petroleum ether: ethyl acetate = 4: 1, volume ratio) to obtain 8.36 g of a brown liquid.

[0120] d) 4-chloro-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one At 20°C, methyl 3-(4,6-dichloropyrimidin-5-yl)butyrate (4.12g) and aqueous ammonia solution (25-28%wt, 25mL) were placed in a 100mL autoclave, heated to 60°C, and reacted for 18 hours. After the reaction was complete, the reaction solution was cooled to 0°C, suction filtered, and the filter cake was pulped with 30mL (petroleum ether:ethyl acetate=10:1, volume ratio) to obtain 1.51g of a pale yellow solid. 1 H NMR (300MHz, DMSO-d6) δ (ppm) 1.09-1.12 (d, J = 7.2 Hz, 3H), 2.36-2.49 (m, 1H), 2.92-3.00 (m, 1H), 3.27-3.36 (m, 1H), 8.54 (s, 1H).

[0121] e) t-Butyl 4-(5-methyl-7-oxo-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-4-yl)piperazine-1-carboxylate Under a nitrogen gas atmosphere, 4-chloro-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one (0.42 g) and piperazine-1-carboxylate t-butyl (10.76 g) were heated to 140 °C at 22 °C and stirred for 6 hours. After the reaction was completed, the reaction solution was cooled to 0 °C, poured into 30 mL of ice water, and adjusted to pH = 7 by adding 4 M hydrochloric acid. The mixture was extracted with dichloromethane (20 mL x 2), the solvent was removed by vacuum distillation, and the product was separated by silica gel column chromatography (dichloromethane:methanol = 30:1, volume ratio) to obtain 0.67 g of a pale yellow solid. LC-MS (ESI) m / z: 348, (M + H). 1 H NMR(300MHz, CDCl3)δ(ppm)8.47(s,1H),8.42(s,1H),3.24-3.61(m,9H),2.56-2.83(m,2H),1.48(s,9H),1.23-1.26(d,J=6.9Hz,3H).

[0122] f) 5-Methyl-4-(piperazin-1-yl)-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one At 25°C, 4-(5-methyl-7-oxo-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-4-yl)piperazine-1-carboxylate t-butyl (0.67g) was dissolved in dichloromethane (10mL), trifluoroacetic acid (1.31g) was added and reacted for 16 hours. After the reaction was completed, the solvent was removed by vacuum distillation, cooled to 0°C, adjusted to pH = 12 with 20% sodium hydroxide solution, extracted with dichloromethane (20mL x 6), the organic phase was combined, the solvent was removed by vacuum distillation, and separated by silica gel column chromatography (dichloromethane:methanol = 20:1, volume ratio) to obtain 0.39g of a yellow solid, which was used as it is in the next step.

[0123] g) 4-(4-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)piperazin-1-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one Under a nitrogen gas atmosphere, 5-methyl-4-(piperazin-1-yl)-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one (0.10 g) and (S)-2-(4-chlorophenyl)-3-(isopropylamino)propionic acid (0.107 g) were dissolved in dichloromethane (5 mL) at 20°C, and 2-(7-benzotriazole oxide)-N,N,N',N'-tetramethyluronium hexafluorophosphate (0.184 g) and diisopropylethylamine (0.078 g) were added, followed by reaction at 25°C for 16 hours. After the reaction was completed, 10 mL of water was added to the reaction solution to separate the liquid, the organic phase was washed with saturated saline (2 mL), the solvent was removed by distillation under reduced pressure, and the solid was isolated by silica gel column chromatography (dichloromethane:methanol = 25:1, volume ratio) to obtain 0.12 g of a white solid. LC-MS (ESI) m / z: 471 (M+H). 1H NMR(300MHz,CDCl3)δ(ppm)1.06-1.28(m,6H),1.30-1.38(m,3H),2.57(d,J=15.0Hz,1H),2.70-2.78(m,4 H),2.80-3.25(m,2H),3.28-3.69(m,7H),3.86-4.09(m,2H),7.22-7.36(m,4H),8.38(s,1H),8.61(s,1H).

[0124] Example 2: (S)-4-(4-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)piperazin-1-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one and (R)-4-(4-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)piperazin-1-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one [ka] Under a nitrogen gas atmosphere, 5-methyl-4-(piperazin-1-yl)-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one (0.49 g) and (S)-2-(4-chlorophenyl)-3-(isopropylamino)propionic acid (0.53 g) were dissolved in N,N-dimethylformamide (20 mL) at 20°C, and 2-(7-benzotriazole oxide)-N,N,N',N'-tetramethyluronium hexafluorophosphate (1.50 g) and diisopropylethylamine (0.51 g) were added, followed by reaction at 25°C for 4 hours. After the reaction was completed, 100 mL of water was added to the reaction solution to separate the liquid, the organic phase was washed with saturated saline (40 mL), the solvent was removed by distillation under reduced pressure, and the product was isolated by silica gel column chromatography (dichloromethane:methanol = 25:1, volume ratio) to obtain 0.59 g of a white solid. Optical resolution gave 0.21 g (de% = 100%) of the (S)-configured product and 0.19 g (de% = 99%) of the (R)-configured product.

[0125] Optical segmentation equipment: waters SFC200, カラム: Daicel Chiralcel OD, 250×30mm ID, 5μm, mobile phase A:CO2, mobile phase B: イソプロパノール (0.1%NH3·H2Oをcontainingむ), A:B=70:​​30 (volume ratio). (S) Configuration product: LC-MS (ESI) m / z: 471 (M+H). 1 H NMR(300MHz,DMSO-d6)δ(ppm)0.93-0.96(m,6H),1.30(d,J=3.0Hz,3H),2.23-2.36(m,1H),2.61-2.84(m,4H),3.12-3.31(m,5H), 3.35-3.49(m,2H),3.65-3.78(m,3H),4.21-4.29(m,1H),7.33(d,J=6.6Hz,2H),7.40(d,J=6.6Hz,2H),8.38(s,1H),10.66(s,1H), (R) Configuration product: LC-MS (ESI) m / z: 471 (M+1). 1 H NMR(300MHz,DMSO-d6)δ(ppm)0.95-1.03(m,9H),2.26-2.30(m,1H),2.66-2.79(m,1H),2.82-2.96(m,3H),3.11-3.20(m,4H),3. 34-3.47(m,3H),3.62-3.69(m,3H),4.18-4.22(m,1H),7.34(d,J=6.6Hz,2H),7.42(d,J=6.6Hz,2H),8.28(s,1H),10.62(s,1H).

[0126] スキームB:

change

[0127] Example 3: (S)-4-(4-(2-(4-chlorophenyl)-3-(isopropylamino)propionyl)piperazin-1-yl)-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one [ka] Prepared similarly to the method described in Example 1, except using methyl acrylate instead of ethyl crotonate. LC-MS (ESI) m / z: 457 (M+H). 1 H NMR(300MHz,DMSO-d6)δ(ppm)0.92-0.96(m,6H),2.42-2.49(m,2H),2.62-2.78(m,5H),3.08-3.29(m,4H),3.38-3 .43(m,2H),3.57-3.65(m,3H),4.21-4.29(m,1H)7.31-7.34(m,2H),7.38-7.40(m,2H),8.27(s,1H),10.53(s,1H).

[0128] Example 4 4-((R)-4-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)-2-methylpiperazin-1-yl)-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one [ka] Prepared similarly to the method described in Example 3, except that t-butyl (R)-3-methylpiperazine-1-carboxylate was used instead of t-butyl piperazine-1-carboxylate. LC-MS (ESI) m / z: 471 (M+H). 1 H NMR(300MHz,DMSO-d6)δ(ppm)0.75-0.95(m,9H),2.40-2.49(m,3H),2.51-2.61(m,4H),2.85-3.36(m,5H),3. 62-3.76(m,1H),3.91-3.96(m,1H),4.14-4.19(m,2H)7.29-7.42(m,4H),8.26(d,J=7.2Hz,1H),10.54(s,1H).

[0129] Example 5 4-((S)-4-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)-2-methylpiperazin-1-yl)-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one [ka] Prepared in a manner similar to that described in Example 3, except that t-butyl piperazine-1-carboxylate was replaced by (S)-3-methylpiperazine-1-carboxylate. LC-MS (ESI) m / z: 471 (M+H). 1 H NMR(300MHz,DMSO-d6)δ(ppm)1.10-1.33(m,9H),2.42-2.49(m,2H),2.67-2.76((m,4H),2.84-3.05(m,4H) ),3.51-3.58(m,3H),3.91-4.19(m,2H),4.28-4.54(m,1H)7.32-7.45(m,4H),8.27(s,1H),10.53(s,1H).

[0130] Scheme C: [ka] Reaction conditions: a) ethyl crotonate, sodium methoxide in methanol (30% wt), methanol, b) disodium hydrogen phosphate, deionized water, hydrochloric acid, lipase (Candida rugosa), sodium hydroxide, c) formamidine acetate, sodium methoxide, methanol, d) phosphoryl chloride, diisopropylethylamine, acetonitrile, e) aqueous ammonia solution (25-28% wt). Intermediate (R)-4-Chloro-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one [ka] a) Trimethyl 2-methylpropane-1,1,3-tricarboxylate Under a nitrogen gas atmosphere, a sodium methoxide methanol solution (30%wt, 50.32g) was added to methanol (900mL) at 20°C, and then the temperature was raised to 70°C. Dimethyl malonate (461.12g) and ethyl crotonate (349.46g) were mixed uniformly and added dropwise to the sodium methoxide methanol solution, and reacted for 3 hours at 70°C. After the reaction was completed, the solvent was removed by vacuum distillation, ethyl acetate (1L) was added, the pH was adjusted to 7-8 with 4M hydrochloric acid, and 500mL of water was added, followed by liquid separation, and the organic phase was removed by vacuum distillation to obtain 777.68g of a yellow liquid. 1 H NMR(400MHz,DMSO-d6)δ(ppm)3.67(s,3H),3.65(s,3H),3.59(s,3H),3.56(d ,J=6.8Hz,1H),2.45-2.58(m,2H),2.23-2.29(m,1H),0.93(d,J=6.8Hz,3H).

[0131] b) (R)-2-Methylpropane-1,1,3-tricarboxylate trimethyl Dissolve disodium hydrogen phosphate (4.5g) in 1.5L of deionized water at 25℃, adjust pH=7.05 with 2N hydrochloric acid, add trimethyl 2-methylpropane-1,1,3-tricarboxylate (150.46g) and lipase (Candida rugosa, add 40g over 6 days), adjust pH to 7.0-7.6 with 2N sodium hydroxide, react at 35℃ for 6 days, chirality measurement ee%>98%, chirality measurement conditions (Chiralpak IC, 4.6×250mm, 5μm, n-hexane:ethanol=9:1, volume ratio). The reaction solution was cooled to 10°C, the pH was adjusted to 3-4 with 3M hydrochloric acid, 500mL of ethyl acetate was added, and the mixture was suction filtered. The filter cake was washed with ethyl acetate (600mL) and separated, and then washed with saturated aqueous sodium bicarbonate (100mL) and separated. The organic phase was concentrated to obtain 26.89g of a pale yellow liquid. 1 H NMR(400MHz,CDCl3)δ(ppm)3.74(s,6H),3.68(s,3H),3.46(d,J=7.2Hz,1H),2.71-2.79( m,1H),2.54(dd,J=15.6,4.8Hz,1H),2.32(dd,J=16.0,8.4Hz,1H),1.06(d,J=6.8Hz,3H).

[0132] c) (R)-3-(4,6-dihydroxypyrimidin-5-yl)butyric acid methyl ester Under a nitrogen gas atmosphere, formamidine acetate (11.33 g) was dissolved in methanol (200 mL) at 20 ° C., cooled to 0 ° C., sodium methoxide methanol solution (30%, 55.62 g) was added dropwise, and the mixture was reacted at 0 ° C. for 60 min., (R)-2-methylpropane-1,1,3-tricarboxylate trimethyl (24.07 g) in methanol (60 mL) was added dropwise, and the mixture was allowed to naturally warm to 20 ° C. and reacted for 10 hours. After the reaction was completed, the reaction solution was cooled to 0 ° C., 3N hydrochloric acid was added to adjust the pH to 5-6, the solvent was removed by distillation under reduced pressure, and then cooled to 0 ° C., 3N hydrochloric acid was added to adjust the pH to 3, and a solid precipitated. The solid was collected by suction filtration, the filter cake was washed with ice water (100 mL), and then vacuum dried to obtain 18.79 g of a white solid, which was used as it was in the next step.

[0133] d) (R)-3-(4,6-dichloropyrimidin-5-yl)butyric acid methyl ester Under a nitrogen gas atmosphere, methyl (R)-3-(4,6-dihydroxypyrimidin-5-yl)butyrate (14.63 g) was dispersed in acetonitrile (70 mL) at 22 ° C., phosphoryl chloride (26.42 g) and diisopropylethylamine (12.51 g) were added dropwise in this order, the reaction system was heated vigorously, and the temperature rose to 60 ° C., the solid gradually dissolved completely, and the reaction was continued for 18 hours. After the reaction was completed, the reaction solution was cooled to 0 ° C., 100 mL of ethyl acetate was added, the pH was adjusted to 7-8 with saturated sodium bicarbonate water, extracted with ethyl acetate (50 mL × 3), the organic phase was removed by distillation under reduced pressure, and 13.89 g of a yellow solid was obtained, which was used as it was in the next step.

[0134] e) (R)-4-chloro-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one At 20°C, methyl (R)-3-(4,6-dichloropyrimidin-5-yl)butyrate (13.89 g) and aqueous ammonia (25-28% wt, 70 mL) were placed in a 100 mL autoclave, heated to 50°C, and reacted for 18 hours. After the reaction was complete, the reaction solution was cooled to 0°C, suction filtered, and the filter cake was pulped with 30 mL (petroleum ether: ethyl acetate = 10:1, volume ratio) to obtain 7.32 g of a pale yellow solid. LC-MS (ESI) m / z: 198 (M+H). 1 H NMR(300MHz, CDCl3)δ(ppm)1.30(d,J=7.2Hz,3H),2.65-2.69(m,1H),2.86-2.92(m,1H),3.47-3.54(m,1H),8.64(s,1H),10.10(s,1H).

[0135] Scheme D: [ka] Reaction conditions: a) t-Butyl 2,5-diazabicyclo[2.2.1]heptane-2-carboxylate, 4-dimethylaminopyridine, N-methylpyrrolidone, b) hydrogen chloride / 1,4-dioxane (4.0 M), dioxane, c) (S)-2-(4-chlorophenyl)-3-(isopropylamino)propionic acid, 2-(7-benzotriazole oxide)-N,N,N',N'-tetramethyluronium hexafluorophosphate, diisopropylethylamine, N,N-dimethylformamide.

[0136] Example 6 (5R)-4-(5-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)-2,5-diazabicyclo[2.2.1]heptan-2-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one [ka] a) t-Butyl 4-(5-methyl-7-oxo-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-4-yl)piperazine-1-carboxylate Under a nitrogen gas atmosphere at 22°C, (R)-4-chloro-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one (0.30g), 2,5-diazabicyclo[2.2.1]heptane-2-carboxylate t-butyl (1.21g), and 4-dimethylaminopyridine (0.58g) were dissolved in N-methylpyrrolidone, heated to 140°C, and reacted for 6 hours. After the reaction was completed, the reaction solution was cooled to 0°C and poured into 30mL of ice water, 4M hydrochloric acid was added to adjust the pH to 4-5, extracted with ethyl acetate (20mL x 2), the organic phase was washed with saturated saline (10mL x 3), the organic phase was removed by distillation under reduced pressure, and 0.33g of a pale yellow solid was obtained by silica gel column chromatography (petroleum ether: ethyl acetate = 1:1, volume ratio). LC-MS(ESI) m / z: 360(M+H).

[0137] b) (5R)-4-(2,5-diazabicyclo[2.2.1]heptan-2-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one At 25°C, 4-(5-methyl-7-oxo-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-4-yl)piperazine-1-carboxylate t-butyl (0.28 g) was dissolved in dioxane (3 mL), and 1.91 g of hydrogen chloride / 1,4-dioxane (4.0 M) was added and reacted for 16 hours. After the reaction was completed, the reaction solution was concentrated to remove the solvent, cooled to 0°C, and adjusted to pH = 12 by adding 20% ​​sodium hydroxide solution, extracted with dichloromethane (20 mL x 8), and the organic phase was removed by distillation under reduced pressure to obtain 0.15 g of a brown solid, which was used as it is in the next step.

[0138] c) (5R)-4-(5-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)-2,5-diazabicyclo[2.2.1]heptan-2-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one Under a nitrogen gas atmosphere at 20°C, 4-(5-methyl-7-oxo-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-4-yl)piperazine-1-carboxylate t-butyl (0.15 g) and (S)-2-(4-chlorophenyl)-3-(isopropylamino)propionic acid (0.157 g) were dissolved in N,N-dimethylformamide (5 mL), and 2-(7-benzotriazole oxide)-N,N,N',N'-tetramethyluronium hexafluorophosphate (0.26 g) and diisopropylethylamine (0.29 g) were added, followed by reaction at 25°C for 16 hours. After the reaction was completed, 10 mL of water was added to the reaction solution to separate the liquid, the organic phase was washed with saturated saline (2 mL), the organic phase was removed by distillation under reduced pressure, and 0.037 g of a tan solid was obtained by silica gel column chromatography (dichloromethane:methanol = 20:1, volume ratio). LC-MS (ESI) m / z: 483 (M+H). 1H NMR(300MHz,DMSO d6)δ(ppm)0.68-1.24(m,10H),1.75-2.38(m,3H),2.54-2.61(m,4H),3.08-3.28(m,2H),3.59-3.61(m,2H) ,3.87-4.16(m,2H),4.72-4.97(m,2H)7.31-7.38(m,4H),8.10(d,J=23.1Hz,1H),10.36(d,J=11.4Hz,1H).

[0139] Example 7 (R)-4-((R)-4-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)-2-methylpiperazin-1-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one [ka] Prepared similarly to the method described in Example 6, except using (R)-4-Boc-2-methylpiperazine instead of t-butyl 2,5-diazabicyclo[2.2.1]heptane-2-carboxylate. LC-MS (ESI) m / z: 485 (M+H). 1 H NMR(300MHz,DMSO-d6)δ(ppm)0.94-1.04 (m,12H),2.24-2.29(m,1H),2.67-2.93(m,5H),3.06-3.19(m,5H),3.57-3. 62(m,3H),4.21-4.24(m,1H),7.32-7.44(m,4H),8.26(s,1H),10.61(s,1H).

[0140] Example 8 (R)-4-((R)-4-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)-3-methylpiperazin-1-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one [ka] Prepared similarly to the method described in Example 6, except using (R)-1-N-Boc-2-methylpiperazine instead of t-butyl 2,5-diazabicyclo[2.2.1]heptane-2-carboxylate.

[0141] LC-MS(ESI)m / z:485(M+H). 1 H NMR(300MHz,CDCl3)δ(ppm)8.37(d,J=12.0Hz,1H),8.28(s,1H),7.36(d,J=6.0Hz,2H),7.26(d,J=6.0Hz,2H),4.59-4.95( m,1H),4.08-4.31(m,2H),3.15-3.82(m,6H),2.72-3.09(m,4H),2.52-2.58(m,1H),2.32-2.39(m,1H),1.10-1.38(m,12H).

[0142] Example 9 (R)-4-((S)-4-((R)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)-3-methylpiperazin-1-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one [ka] Prepared similarly to the method described in Example 6, except using (S)-1-N-Boc-2-methylpiperazine instead of t-butyl 2,5-diazabicyclo[2.2.1]heptane-2-carboxylate. LC-MS (ESI) m / z: 485 (M+H). 1 H NMR(400MHz,CDCl3)δ(ppm)8.35(d,J=16.0Hz,1H),7.41-7.28(m,3H),7.26-7.2 1(m,1H),4.89(d,J=6.8Hz,1H),4.76-4.49(m,1H),3.75-3.65(m,1H),3.64-3.54 (m,1H),3.51-3.49(m,1H),3.44-3.34(m,1H),3.34-3.17(m,3H),3.17-3.09(m, 1H), 3.06-2.96 (m, 1H), 2.89-2.49 (m, 3H), 1.44-1.29 (m, 7H), 1.25-1.06 (m, 5H).

[0143] Example 10 (R)-4-(8-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)-3,8-diazabicyclo[3.2.1]octan-3-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one [ka] Prepared similarly to the method described in Example 6, except for using t-butyl 3,8-diazabicyclo[3.2.1]octane-8-carboxylate instead of t-butyl 2,5-diazabicyclo[2.2.1]heptane-2-carboxylate. LC-MS (ESI) m / z: 497 (M+H). 1 H NMR(400MHz,CDCl3)δ(ppm)8.35(d,J=16.0Hz,1H),7.41-7.28(m,3H),7.26-7.21(m, 1H),4.83(s,1H),4.51-4.30(m,2H),4.25-4.22(m,1H),4.10-4.03(m, 1H),3.92-3.84(m,1H),3.73-3.62(m,1H),3.50-3.26(m,3H),3.24-3.2 1(m,1H),3.20-2.85(m,2H),2.75-2.62(m,1H),2.49-2.41(m,1H),2.20 -1.90(m,1H),1.88-1.60(m,3H),1.44-1.29(m,6H),1.25-1.06(m,3H).

[0144] Example 11 (R)-4-(3-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)-3,8-diazabicyclo[3.2.1]octan-8-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one [ka] Prepared similarly to the method described in Example 6, except using t-butyl 3,8-diazabicyclo[3.2.1]octane-3-carboxylate instead of t-butyl 2,5-diazabicyclo[2.2.1]heptane-2-carboxylate. LC-MS (ESI) m / z: 497 (M+H). 1 H NMR(400MHz,CDCl3)δ(ppm)8.66(s,1H),8.28(d,J=5.2Hz,1H),7.15-7.38(m,4H),4.31-4.56(m,3H),3.93(s,1H),3.49-3.66(m,2H), 3.15-3.31(m,2H),2.70-3.01(m,4H),2.26-2.50(m,1H),1.74-2.03(m,3H),1.57-1.67(m,1H),1.43-1.50(m,1H),1.03-1.26(m,9H).

[0145] Example 12 (R)-4-(4-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)-4,7-diazaspiro[2.5]octan-7-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one [ka] Prepared similarly to the method described in Example 6, except using t-butyl 4,7-diazaspiro[2.5]octane-4-carboxylate instead of t-butyl 2,5-diazabicyclo[2.2.1]heptane-2-carboxylate. LC-MS (ESI) m / z=497 (M+H). 1 H NMR(400MHz,DMSO-d6)δ10.58(s,1H),8.22(s,1H),7.33-7.38(m,4H),4.37(s,1H),3.40- 4.15(m,4H),2.97-3.09(m,3H),2.57-2.90(m,4H),2.24-2.32(m,1H),0.44-1.13(m,13H).

[0146] Example 13 (5R)-4-(5-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)hexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one [ka] The same method as described in Example 6 was used to prepare the product, except that hexahydropyrrolo[3,4-c]pyrrole-2(1H)-carboxylate was used instead of t-butyl 2,5-diazabicyclo[2.2.1]heptane-2-carboxylate. The final product prepared was optically resolved to obtain cis and trans isomers. Conditions for optical resolution: Equipment for resolution: waters SFC200, column: Daicel Chiralcel AD, 250×30 mm ID, 5 μm, mobile phase A: CO2, mobile phase B: ethanol (containing 0.1% aqueous ammonium solution), A:B=65:35 (volume ratio). Products with retention times of 8-10 min were collected, which were cis isomers, and products with retention times of 11-13 min were collected, which were trans isomers.

[0147] Cis isomer: LC-MS (ESI) m / z: 497.(M+H). 1 H NMR(300MHz,DMSO-d6)δ(ppm)10.31(d,J=6.9Hz,1H),8.08(d,J=13.5Hz,1H), 7.28-7.38(m,4H),3.76-3.93(m,3H),3.56-3.72(m,2H),3.45-3.52(m,2H),3 .38-3.40(m,2H),3.13-3.24(m,2H),3.03-3.07(m,1H),2.84-2.90(m,2H),2. 57-2.78(m,3H),2.17-2.26(m,1H),1.04(d,J=5.1Hz,2H),0.87-0.93(m,7H).

[0148] Trans isomer: LC-MS(ESI) m / z: 497.(M+H). 1H NMR(300MHz,DMSO-d6)δ(ppm)10.31(d,J=7.8Hz,1H),8.08(d,J=13.2Hz,1H),7.28-7.40(m,4H),3.82-3.94(m,2H),3.68-3.79(m,1H),3.47-3.63 (m,2H),3.07-3.22(m,3H),2.62-3.02(m,5H),2.18-2.27(m,1H),1.20-1 .29(m,3H),1.05(d,J=5.4Hz,1H),0.90-0.96(m,6H),0.81-0.86(m,3H).

[0149] Example 14 (R)-4-(6-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)-2,6-diazaspiro[3.3]heptan-2-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one [ka] Prepared similarly to the method described in Example 6, except using t-butyl 2,6-diazaspiro[3.3]heptane-2-carboxylate instead of t-butyl 2,5-diazabicyclo[2.2.1]heptane-2-carboxylate. LC-MS (ESI) m / z: 483 (M+H). 1 H NMR(400MHz,DMSO-d6)δ(ppm)10.38(s,1H),8.09(s,1H),7.36-7.38(m,2H),7.30-7.32(m,2H),4.48(d,J=9.6Hz,1H),4.35(s,2H),4.26(s,2H) ,3.98-4.08(m,3H),3.63-3.67(m,1H),3.00-3.10(m,2H),2.61-2.73(m ,4H),2.23(d,J=16.0Hz,1H),1.02(d,J=7.2Hz,3H),0.91-0.93(m,6H).

[0150] Scheme E [ka] Reaction conditions: a) t-butyl 2,5-diazabicyclo[4.1.0]heptane-2-carboxylate, N-methylpyrrolidone, 4-dimethylaminopyridine, b) hydrogen chloride / 1,4-dioxane (4.0 M), dichloromethane, c) (S)-3-(t-butoxycarbonyl)(isopropyl)amino)-2-(4-chlorophenyl)propionic acid, 2-(7-benzotriazole oxide)-N,N,N',N'-tetramethyluronium hexafluorophosphate, 4-dimethylaminopyridine, N,N-dimethylformamide, d) trifluoroacetic acid, dichloromethane.

[0151] Example 15 (R)-4-((1S,6R)-5-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)-2,5-diazabicyclo[4.1.0]heptan-2-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one or (R)-4-((1R,6S)-5-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)-2,5-diazabicyclo[4.1.0]heptan-2-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one [ka] a) t-Butyl 5-((R)-5-methyl-7-oxo-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-4-yl)-2,5-diazabicyclo[4.1.0]heptane-2-carboxylate Under a nitrogen gas atmosphere, (R)-4-chloro-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one (0.21 g), 2,5-diazabicyclo[4.1.0]heptane-2-carboxylate t-butyl (0.31 g), and 4-dimethylaminopyridine (0.39 g) were dissolved in N-methylpyrrolidone (5 mL) at 22 ° C., and then heated to 140 ° C. and reacted for 3 hours. After the reaction was completed, the reaction solution was cooled to 20 ° C. and poured into 20 mL of ice water, extracted with ethyl acetate (20 mL × 2), washed with saturated saline (10 mL × 3), the solvent was removed by distillation under reduced pressure, and separated by silica gel column chromatography (petroleum ether: ethyl acetate = 3: 1 to 1: 1) to obtain 0.28 g of a pale yellow liquid. LC-MS (ESI) m / z: 360 (M + H).

[0152] b) (5R)-4-(2,5-diazabicyclo[4.1.0]heptan-2-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one hydrochloride At 20°C, 5-((R)-5-methyl-7-oxo-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-4-yl)-2,5-diazabicyclo[4.1.0]heptane-2-carboxylate t-butyl (0.28 g) was dissolved in dichloromethane (5 mL), and hydrogen chloride / 1,4-dioxane (4.0 mL) was added and reacted for 1 hour. After the reaction was complete, the reaction solution was distilled under reduced pressure to remove the solvent, obtaining 0.23 g of a yellow solid, which was used as is in the next step.

[0153] c) (2S)-2-(4-chlorophenyl)-3-(5-((R)-5-methyl-7-oxo-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-4-yl)-2,5-diazabicyclo[4.1.0]heptan-2-yl)-3-oxopropyl)(isopropyl)carbamate t-butyl Under a nitrogen gas atmosphere at 20°C, (5R)-4-(2,5-diazabicyclo[4.1.0]heptan-2-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one hydrochloride (0.20 g) and (S)-3-((t-butoxycarbonyl)(isopropyl)amino)-2-(4-chlorophenyl)-propionic acid (0.22 g) were dissolved in N,N-dimethylformamide (5 mL), and 2-(7-benzotriazole oxide)-N,N,N',N'-tetramethyluronium hexafluorophosphate (0.59 g) and 4-dimethylaminopyridine (0.48 g) were added and reacted at 25°C for 4 hours. After the reaction was completed, 20 ml of water was added to the reaction solution, and the mixture was extracted with ethyl acetate (10 mL x 3). The organic phase was washed with saturated saline (10 mL x 2), and the organic phase was removed by vacuum distillation. The product was separated by silica gel column chromatography (dichloromethane:methanol = 50:1) to obtain 0.18 g of a yellow solid. LC-MS (ESI) m / z: 583 (M + H).

[0154] d) (R)-4-((1S,6R)-5-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)-2,5-diazabicyclo[4.1.0]heptan-2-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one or (R)-4-((1R,6S)-5-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)-2,5-diazabicyclo[4.1.0]heptan-2-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one At 20°C, (2S)-2-(4-chlorophenyl)-3-(5-((R)-5-methyl-7-oxo-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-4-yl)-2,5-diazabicyclo[4.1.0]heptan-2-yl)-3-oxopropyl)(isopropyl)carbamate t-butyl (0.18g) was dissolved in dichloromethane (2mL), trifluoroacetic acid (0.86mL) was added, and the mixture was reacted for 3 hours. After the reaction was completed, dichloromethane (10mL) was added to the reaction solution, and 2M sodium hydroxide solution was added dropwise at 0°C to adjust the pH to 12, followed by separation. The organic phase was washed with saturated saline (5mL), dried over anhydrous sodium sulfate, and the organic phase was removed by distillation under reduced pressure to obtain 0.10g of a yellow solid. The mixture was separated by high-performance preparative liquid chromatography to give isomer 1 (3 mg) and isomer 2 (12 mg). High-performance preparative liquid chromatography conditions: Column: Aglient 5 μm prep-C1850×21.2 mm, Mobile phase A: water (containing 0.1% aqueous ammonia), Mobile phase B: methanol. Gradient: Time 0-10 min, B phase 60-70% (volume ratio).

[0155] Isomer 1: RT1=5.3min, LC-MS(ESI) m / z:483(M+H).

[0156] Isomer 2: RT2=5.9min, LC-MS(ESI) m / z:483(M+H), 1 H NMR(400MHz,CDCl3)δ(ppm)8.27(d,J=7.6Hz,1H),7.92(s,1H),7.27-7.30(m,4H),4.23- 4.29(m,1H),3.90-3.95(m,1H),3.81-3.85(m,1H),3.69-3.72(m,1H),3.44-3.59(m,1H), 3.20-3.38(m,3H),3.01-3.05(m,1H),2.70-2.85(m,3H),2.47-2.57(m,1H), 2.21-2.25(m,1H),1.25-1.28(m,3H),1.03-1.11(m,6H),0.82-0.90(m,2H).

[0157] Structure determination by single crystal diffraction: Preparation of single crystals: 30.0 mg of isomer 2 compound and 2.0 mL of isopropanol were placed in a 5 mL screw-top glass bottle and stirred for 5 min until the solid was completely dissolved. 3.9 mg of oxalic acid dihydrate was weighed and placed in the above glass bottle, and a white solid gradually precipitated in the glass bottle. Stirred at room temperature for 3 h, and a large amount of white solid precipitated in the glass bottle. 1.0 mL of methanol was added to the glass bottle, and the white solid gradually disappeared and the solution became transparent, followed by stirring for 1 h. The solution was passed through a 0.22 μm microporous filter membrane and filtered into a 3 mL screw-top glass bottle, and the mouth of the glass bottle was covered with plastic wrap. Eight holes were made in the mouth of the bottle with a needle, and the bottle was left at room temperature for 7 days to obtain a single crystal of the oxalate salt of isomer 2 compound. JPEG0007617005000087.jpg90166

[0158] Structure description: Single crystal X-ray diffraction and structure analysis show that the obtained single crystal is an isopropanol solvate of oxalate salt of isomer 2. The asymmetric structural unit of the crystal contains four molecules of isomer 2, two molecules of oxalic acid and two molecules of isopropanol, and isomer 2 and oxalic acid form the oxalate salt. The schematic diagram of a single molecule of compound isomer 2 is shown in Figure 1, and the asymmetric structural unit of the oxalate salt single crystal is shown in Figure 2. The structural formula is shown below: [ka]

[0159] Scheme F: [ka] Reaction conditions: a) methyl 4,4,4-trifluorobut-2-enoate, thorium methoxide in methanol (30% wt), b) formamidine acetate, sodium methoxide in methanol (30% wt), c) phosphoryl chloride, diisopropylethylamine, acetonitrile, d) aqueous ammonia (25-28% wt), e) t-butyl piperazine-1-carboxylate, f) trifluoroacetic acid, dichloromethane, g) (S)-2-(4-chlorophenyl)-3-(isopropylamino)propionic acid, 2-(7-benzotriazole oxide)-N,N,N',N'-tetramethyluronium hexafluorophosphate, diisopropylethylamine, dichloromethane.

[0160] Example 16: 4-(4-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)piperazin-1-yl)-5-trifluoromethyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one [ka] Prepared similarly to the method described in Example 1 according to Scheme F. LC-MS (ESI) m / z: 525 (M+H). Separation of isomers: The above title compound was optically resolved by supercritical fluid chromatography to obtain isomer 1 and isomer 2. Equipment and conditions for optical resolution: Waters SFC200, column: Daicel Chiralcel AS, 250×30 mm ID, 5 μm, mobile phase: A is CO2, B is ethanol (0.1% NH3H2O), A:B=85:15 (volume ratio), flow rate 60 mL / min, column temperature 38°C.

[0161] Isomer 1: [ka] LCMS(ESI)m / z:525(M+H). 1HNMR(400MHz,DMSO-d6)δ(ppm):0.91-0.95,(m,6H),2.59-2.78(m,4H),3.07-3.12(m,1H),3.20-3.31(m,4H),3. 37-3.50(m,3H),3.69-3.75(m,2H),4.08-4.19(m,2H),7.35(dd,J=25.2,8.0Hz,4H),8.36(s,1H),10.90(s,1H).

[0162] Isomer 2: [ka] LCMS(ESI)m / z:525(M+H). 1 HNMR(300MHz,DMSO-d6)δ(ppm)0.93-1.06(m,6H),2.58-2.72(m,4H),3.06-3.31(m,4H),3. 45-3.68(m,6H),4.11-4.20(m,2H)7.31-7.43(m,4H),8.34(d,J=6.0Hz,1H),10.92(s,1H).

[0163] Scheme G: [ka] Reaction conditions: a) t-butyl 4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,6-dihydropyridine-1(2H)-carboxylate, dioxane, cesium carbonate, water, tetrakis(triphenylphosphine)palladium, b) methanol, formic acid, palladium on carbon (5%), hydrogen gas, c) dichloromethane, hydrogen chloride / 1,4-dioxane solution (4.0M), d) (S)-3-((t-butoxycarbonyl)(isopropyl)amino)-2-(4-chlorophenyl)propionic acid, N,N-dimethylformamide, 2-(7-benzotriazole oxide)-N,N,N',N'-tetramethyluronium hexafluorophosphate, diisopropylethylamine, e) dichloromethane, hydrogen chloride / 1,4-dioxane solution (4.0M).

[0164] Example 17: [ka] a) (R)-4-(5-methyl-7-oxo-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-4-yl)-3,6-dihydropyridine-1(2H))-carboxylate t-butyl ester Under a nitrogen gas atmosphere at 22°C, (R)-4-chloro-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one (0.60g) and 4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,6-dihydropyridine-1(2H)-carboxylate t-butyl (1.03g) were dissolved in dioxane (20mL), cesium carbonate and water (5mL) were added, and finally tetrakis(triphenylphosphine)palladium (0.35g) was added and reacted at 100°C for 7 hours. After the reaction was completed, the reaction solution was cooled to 20°C and poured into 10mL of ice water, extracted with ethyl acetate (20mL), the solvent was removed by distillation under reduced pressure, and isolated by silica gel column chromatography (petroleum ether: ethyl acetate = 4:1, volume ratio) to obtain 0.81g of a pale yellow solid. MS(ESI) m / z: 345(M+H).

[0165] b) (R)-4-(5-methyl-7-oxo-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-4-yl)piperidine-1-carboxylate t-butyl ester At 20°C, (R)-4-(5-methyl-7-oxo-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-4-yl)-3,6-dihydropyridine-1(2H))-t-butyl carboxylate (0.75 g) was dissolved in methanol (50 mL), formic acid (0.11 g) and 0.30 g of palladium carbon (5%) were added, the atmosphere was replaced with nitrogen gas three times, hydrogen gas was poured, and the reaction was carried out at 60°C for 16 hours. After the reaction was completed, the mixture was cooled to 10°C, the palladium carbon was filtered by suction filtration, and the solvent of the filtrate was removed by distillation under reduced pressure to obtain 0.66 g of a colorless oily product, MS (ESI) m / z: 347 (M+H).

[0166] c) (R)-5-Methyl-4-(piperidin-4-yl)-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one At 25°C, (R)-4-(5-methyl-7-oxo-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-4-yl)piperidine-1-carboxylate t-butyl (0.63 g) was dissolved in dichloromethane (10 mL), hydrogen chloride / 1,4-dioxane (4.0 M) (10 mL) was added and reacted for 2 hours. After the reaction was completed, the reaction solution was concentrated to remove the solvent, cooled to 0°C, and adjusted to pH = 12 by adding 20% ​​sodium hydroxide solution, extracted with ethyl acetate (10 mL x 3), washed with saturated saline (15 mL), and the organic phase was removed by distillation under reduced pressure to obtain 0.346 g of a yellow solid, which was used as it is in the next step.

[0167] d) (S)-2-(4-chlorophenyl)-3-(4-((R)-5-methyl-7-oxo-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-4-yl)-piperidin-1-yl)-3-oxopropyl)(isopropyl)carbamate t-butyl Under a nitrogen gas atmosphere at 20°C, (R)-5-methyl-4-(piperidin-4-yl)-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one (0.302 g) and (S)-3-((t-butoxycarbonyl)(isopropyl)amino)-2-(4-chlorophenyl)propionic acid (0.308 g) were dissolved in N,N-dimethylformamide (5 mL), and 2-(7-benzotriazole oxide)-N,N,N',N'-tetramethyluronium hexafluorophosphate (0.86 g) and diisopropylethylamine (0.76 g) were added and reacted at 20°C for 3 hours. After the reaction was completed, 30 mL of water was added to the reaction solution, and the solution was extracted with ethyl acetate (50 mL) and separated. The organic phase was washed with saturated saline (10 mL x 2), and the solvent was removed by distillation under reduced pressure. The product was isolated by silica gel column chromatography (dichloromethane:methanol = 25:1, volume ratio) to obtain 0.339 g of a white solid, which was used directly in the next step.

[0168] e) (R)-4-(1-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)piperidin-4-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one At 25°C, (S)-2-(4-chlorophenyl)-3-(4-((R)-5-methyl-7-oxo-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-4-yl)-piperidin-1-yl)-3-oxopropyl)(isopropyl)carbamate t-butyl (0.320g) was dissolved in dichloromethane (3mL), hydrogen chloride / 1,4-dioxane solution (4.0mL) (2.9mL) was added, and the mixture was reacted for 2 hours. After the reaction was completed, the reaction solution was distilled under reduced pressure to remove the solvent, and then cooled to 0°C, and 20% sodium hydroxide solution was added to adjust the pH to 12, extracted with ethyl acetate (10mL x 3), washed with saturated saline (15mL), and the organic phase was removed by distillation under reduced pressure to obtain 0.176g of a white solid.

[0169] LC-MS(ESI)m / z:470(M+H). 1 H NMR(400MHz,DMSO-d6)δ(ppm)0.83-0.97(m,6H),1.03-1.10(m,3H),1.24-1.43(m,2H),1.57-1.95(m,2H),2.29-2.35(m,1H),2.51-2.87 (m,5H),3.01-3.19(m,3H),3.35-3.41(m,1H),4.02-4.21(m,2H),4.51-4.62(m,1H)7.32-7.43(m,4H),8.50-8.61(m,1H),10.85(s,1H).

[0170] Scheme H: [ka] Reaction conditions: a) t-butyl piperazine-1-carboxylate, N-methylpyrrolidone, 4-dimethylaminopyridine, b) dichloromethane, hydrogen chloride / 1,4-dioxane solution (4.0 M), c) diisopropylethylamine, N,N-dimethylformamide, 2-(7-benzotriazole oxide)-N,N,N',N'-tetramethyluronium hexafluorophosphate, 1-(t-butoxycarbonyl)-4-(4-chlorophenyl)piperidine-4-carboxylic acid, d) dichloromethane, hydrogen chloride / 1,4-dioxane solution (4.0 M).

[0171] Example 18 (R)-4-(4-(4-(4-chlorophenyl)piperidine-4-carbonyl)piperazin-1-yl)-5-methyl-5,6-dihydropyrido[2,3-d]pyrimidin-7(8H)-one formate salt [ka] a) (R)-4-(5-methyl-7-oxo-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-4-yl)piperazine-1-carboxylate t-butyl ester At 20°C, (R)-4-chloro-5-methyl-5,6-dihydropyrido[2,3-d]pyrimidin-7(8H)-one (400mg), piperazine-1-carboxylate t-butyl (1.8g) and 4-dimethylaminopyridine (494mg) were dissolved in N-methylpyrrolidone (10mL). The reaction solution was replaced with nitrogen gas three times, heated to 150°C under a nitrogen gas atmosphere and reacted for 4 hours. After the reaction was completed, the reaction solution was poured into 20mL of ethyl acetate, washed with water (2mL*2), and washed with 0.5M dilute hydrochloric acid to a pH of 5-6. The organic phase was separated, dried over anhydrous sodium sulfate, and concentrated to obtain a crude product. The crude product was isolated and purified using a high-speed column equipment (eluent: petroleum ether / ethyl acetate = 2:1, volume ratio), and 0.6g of a white solid was obtained. LC-MS(ESI) m / z: 348(M+H).

[0172] b) (R)-5-Methyl-4-(piperazin-1-yl)-5,6-dihydropyrido[2,3-d]pyrimidin-7(8H)-one At 18 ° C., (R)-4-(5-methyl-7-oxo-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-4-yl)piperazine-1-carboxylate t-butyl (0.6 g) was dissolved in dichloromethane (2 mL), hydrogen chloride / 1,4-dioxane solution (4.0 M, 5 mL) was added dropwise, and the mixture was reacted at 18 ° C. for 2 hours. After the reaction was completed, the reaction solution was distilled under reduced pressure to remove the solvent, and the oily target product was obtained. The target product was dissolved in dichloromethane, sodium hydroxide solid was added, and the mixture was stirred until the oily liquid was dissolved, and the solid was removed by filtration. The remaining product was concentrated to obtain a white solid (0.3 g), which was used as it was in the next step.

[0173] c) (R)-4-(4-chlorophenyl)-4-(4-(5-methyl-7-oxo-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-4-yl)-piperazine-1-carbonyl)piperidine-1-carboxylate t-butyl ester At 18°C, (R)-5-methyl-4-(piperazin-1-yl)-5,6-dihydropyrido[2,3-d]pyrimidin-7(8H)-one (150mg), 1-(t-butoxycarbonyl)-4-(4-chlorophenyl)piperidine-4-carboxylic acid (247mg), diisopropylethylamine (235mg) and 2-(7-benzotriazole oxide)-N,N,N',N'-tetramethyluronium hexafluorophosphate (346mL) were dissolved in anhydrous N,N-dimethylformamide (3mL) and reacted for 12 hours at 18°C. After complete reaction, the reaction solution was poured into ethyl acetate (20mL), washed with water (5mL*3), and the organic phase was distilled under reduced pressure to obtain a crude product. The crude product was isolated and purified by silica column chromatography (eluent: petroleum ether / ethyl acetate = 1:2, volume ratio) to obtain 280 mg of the target product as a colorless oil, which was used directly in the next step.

[0174] d) (R)-4-(4-(4-(4-chlorophenyl)piperidine-4-carbonyl)piperazin-1-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one formate At 15°C, (R)-4-(4-chlorophenyl)-4-(4-(5-methyl-7-oxo-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-4-yl)-piperazine-1-carbonyl)piperidine-1-carboxylate t-butyl (280 mg) was dissolved in dichloromethane (5 mL), hydrogen chloride / 1,4-dioxane solution (4.0 mL, 5 mL) was added, and the mixture was reacted at 15°C for 1.5 hours. After the reaction was completed, the reaction solution was concentrated to obtain a white crude product. The crude product was separated by high performance liquid chromatography to obtain 60 mg of the target product. Separation conditions: Column: Kromasil 10 μm C18 50 × 250 mm, mobile phase A: water (containing 0.1% formic acid), mobile phase B: methanol. Gradient: time 0-10min, B phase 20%, 10-30min, B phase 20-50%, 30-40min, B phase 50% (volume ratio), RT=31.4min. LC-MS(ESI)m / z=469[M+H]. 1 H NMR(400MHz,DMSO-d6)δ(ppm)10.62(s,1H),8.41(s,1H),8.26(s,1H),7.48(d,J=8.3Hz,2H),7.29(d,J=8. 3Hz, 2H), 2.74-3.74 (m, 12H), 2.49-2.50 (m, 2H), 2.22-2.26 (m, 3H), 1.96-2.00 (m, 2H), 0.97-0.99 (m, 3H).

[0175] Scheme I: [ka] Reaction conditions: a) methyl methacrylate, sodium methoxide, methanol, b) formamidine acetate, sodium methoxide, methanol, c) phosphoryl chloride, diisopropylethylamine, acetonitrile, d) aqueous ammonia (25-28% wt), e) t-butyl piperazine-1-carboxylate, N,N-dimethylpyridin-4-amine, N-methylpyrrolidone, f) hydrogen chloride / 1,4-dioxane solution (4.0 M), g) (S)-3-((t-butoxycarbonyl)(isopropyl)amino)-2-(4-chlorophenyl)propionic acid, diisopropylethylamine, 2-(7-benzotriazole oxide)-N,N,N',N'-tetramethyluronium hexafluorophosphate, N,N-dimethylformamide, h) hydrogen chloride / 1,4-dioxane solution (4.0 M).

[0176] Example 19 4-(4-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propyl)piperazin-1-yl)-6-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one hydrochloride [ka] a) 1,1,3-tricarboxylate trimethylbutane Under a nitrogen gas atmosphere, sodium methoxide (2.45 g) was dispersed in methanol (50 mL) at 20°C, dimethyl malonate (5 g) and methyl methacrylate (3.75 g) were added, and the mixture was heated to 60°C and reacted for 16 hours. After the reaction was complete, the reaction solution was distilled under reduced pressure to remove the solvent, obtaining 5 g of a yellow liquid, which was used as is in the next step. b) Methyl 3-(4,6-dihydroxypyrimidin-5-yl)-2-methylpropionate Under a nitrogen gas atmosphere, sodium methoxide (11.67 g) was dissolved in methanol (30 mL) at 20 ° C., cooled to 0 ° C., and formamidine acetate (2.44 g) was added while stirring, and reacted for 30 min., and 1,1,3-trimethylbutane tricarboxylate (5 g) was added dropwise and reacted for 16 hours at 20 ° C. After the reaction was completed, isopropanol hydrochloride (4.0 M) was added to the reaction solution to adjust the pH to 5, the solvent was removed by distillation under reduced pressure, and the mixture was cooled to 0 ° C., and a solid was precipitated. The solid was filtered by suction, the filter cake was washed with water (50 mL), and the filter cake was dried to obtain 3.7 g of a pale yellow solid, which was used as it was in the next step.

[0177] c) Methyl 3-(4,6-dichloropyrimidin-5-yl)-2-methylpropionate Under nitrogen gas atmosphere, 3-(4,6-dihydroxypyrimidin-5-yl)-2-methylpropionate methyl (3g) was dispersed in acetonitrile (60mL) at 22℃, phosphoryl chloride (2.91mL) and diisopropylethylamine (1.18mL) were added dropwise in this order, the reaction system was heated violently, the solid gradually dissolved completely, and the temperature was raised to 90℃ and reacted for 16 hours. After the reaction was complete, the reaction solution was poured into water (100mL) and further extracted with ethyl acetate (100mL). The organic phase was washed with saturated saline (100mL*3), dried with anhydrous sodium sulfate, and dried with an evaporator. The product was purified by silica gel column chromatography (petroleum ether:ethyl acetate=10:1, volume ratio) to obtain 1.50g of white solid, which was used as it is in the next step.

[0178] d) 4-chloro-6-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one Methyl 3-(4,6-dichloropyrimidin-5-yl)-2-methylpropionate (1.5 g) was added to an aqueous ammonia solution (3 mL) at 20°C and reacted at 20°C for 16 hours. After the reaction was complete, the reaction solution was poured into water (100 mL) and extracted with ethyl acetate (100 mL). The organic phase was washed with saturated saline (100 mL*3), dried over anhydrous sodium sulfate, and dried in an evaporator. The product was purified by silica gel column chromatography (petroleum ether: ethyl acetate = 4:1, volume ratio) to obtain 0.30 g of a white solid. LC-MS (ESI) m / z: 199 (M+H).

[0179] e) t-Butyl 4-(6-methyl-7-oxo-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-4-yl)piperazine-1-carboxylate At 20°C, t-butyl piperazine-1-carboxylate (0.28g) and N,N-dimethylpyridin-4-amine (0.31g) were added to a solution (3mL) of 4-chloro-6-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one (0.1g) in N-methylpyrrolidone, and the reaction mixture was stirred at 150°C for 3 hours. After complete reaction, the reaction mixture was poured into water (100mL) and extracted with ethyl acetate (100mL). The organic phase was washed with saturated saline (100mL*3), dried over anhydrous sodium sulfate, and the solvent was removed by distillation under reduced pressure. The product was purified by chromatography on silica gel plates (petroleum ether:ethyl acetate = 1:1, volume ratio) to obtain 0.31g of a white solid. LC-MS(ESI)m / z:348.2(M+H).

[0180] f) 6-Methyl-4-(piperazin-1-yl)-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one Hydrogen chloride / 1,4-dioxane solution (4.0M 10mL) was added to t-butyl 4-(6-methyl-7-oxo-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-4-yl)piperazine-1-carboxylate (0.1g) at 20°C, and the reaction mixture was stirred at 20°C for 3 hours. After complete reaction, the solvent was removed by distillation under reduced pressure to give an off-white solid (0.1g). LC-MS (ESI) m / z: 248.2 (M+H).

[0181] g) (2S)-2-(4-chlorophenyl)-3-(4-(6-methyl-7-oxo-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-4-yl)-piperazin-1-yl)-3-oxopropyl)(isopropyl)carbamate t-butyl ester To 6-methyl-4-(piperazin-1-yl)-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one (0.1 g) dissolved in N,N-dimethylformamide (10 mL) at 20°C, (S)-3-((t-butoxycarbonyl)(isopropyl)amino)-2-(4-chlorophenyl)propionic acid (0.17 g) and diisopropylethylamine (0.16 g) were added. The reaction mixture was stirred for 2 min. 2-(7-benzotriazole oxide)-N,N,N',N'-tetramethyluronium hexafluorophosphate (0.23 g) was added at 20°C, and the reaction mixture was stirred at 20°C for 1 h. After the reaction was completed, the reaction mixture was poured into water (100 mL) and extracted with ethyl acetate (100 mL). The organic phase was washed with saturated saline (100mL*3), dried over anhydrous sodium sulfate, and the solvent was removed by distillation under reduced pressure. The product was purified by chromatography on a silica gel plate (petroleum ether:ethyl acetate=1:2, volume ratio) to obtain 0.08g of a white solid, which was used directly in the next step.

[0182] h) 4-(4-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)piperazin-1-yl)-6-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one hydrochloride Hydrogen chloride / 1,4-dioxane solution (4.0 mL, 3 mL) was added to (2R)-2-(4-chlorophenyl)-3-(4-(6-methyl-7-oxo-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-4-yl)-piperazin-1-yl)-3-oxopropyl)(isopropyl) t-butyl carbamate (0.2 g) at 20° C. The reaction mixture was stirred at 20° C. for 3 h. After the reaction was complete, the reaction solution was distilled under reduced pressure to remove the solvent and separated to give 0.06 g of an off-white solid. LC-MS (ESI) m / z: 471 (M+H). 1 H NMR(400MHz,MeOD)δ8.42(d,J=4.5Hz,1H),7.54-7.33(m,4H),4.64-4.52(m,1H),3.96-3.81(m,2H),3.79-3.73(m,2H),3.70-3.66(m,2H),3.6 3-3.56(m,1H),3.51-3.40(m,2H),3.24-3.16(m,1H),3.09-2.94(m,1H) ,2.84(s,1H),2.79-2.66(m,2H),1.44-1.35(m,6H),1.33-1.25(m,3H).

[0183] Separation of isomers: The above-mentioned title compound was optically resolved by supercritical fluid chromatography. Equipment and conditions for optical resolution: Waters SFC200, column: Daicel Chiralcel AS, 250×30 mm ID, 5 μm, mobile phase: A is CO2, B is methanol (0.1% NH3H2O), A:B=90:10 (volume ratio), flow rate 60 mL / min, column temperature 38°C.

[0184] Isomer 1: [ka] LCMS(ESI)m / z:471(M+H). 1H NMR(400MHz, CDCl3)δ8.36(s,1H),7.86(s,1H),7.32-7.37(m,2H),7.21-7.27(m,2 H),4.18(s,1H),3.89-4.00(m,1H),3.65-3.74(m,1H),3.53-3.64(m,2H),3.44-3.5 2(m,1H),3.35-3.42(m,2H),3.27-3.34(m,2H),2.87-2.94(m,1H),2.74-2.82(m,2 H),2.57-2.64(m,1H),2.47-2.54(m,1H),1.32(d,J=6.6Hz,3H),1.07-1.19(m,6H).

[0185] Opposite sex body 2:

change

[0186] スキームJ:

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[0187] Example 20 (S)-4-(4-(2-(4-chlorophenyl)-3-(isopropylamino)propionyl)piperazin-1-yl)-5-methyl-5,8-dihydropteridin-7(6H)-one hydrochloride [ka] a) 2-((4,6-dichloropyrimidin-5-yl)amino)ethyl acetate Sodium hydride (2.93 g) was added to a solution (100 mL) of 4,6-dichloro-5-pyrimidine (10.0 g) dissolved in tetrahydrofuran at 0°C. The reaction mixture was stirred for 2 min. After the temperature naturally rose to 20°C, ethyl bromoacetate (12.22 g) was added, and tetrabutylammonium iodide (27.03 g) was further added. The reaction mixture was stirred at 20°C for 16 h. After the reaction was completed, the reaction solution was poured into water (100 mL), stirred for 30 min, and extracted by adding ethyl acetate (100 mL). The organic phase was washed with saturated saline (100 mL*3), dried over anhydrous sodium sulfate, and the solvent was removed by distillation under reduced pressure. The product was purified by silica gel column chromatography (petroleum ether:ethyl acetate = 20:1, volume ratio) to obtain 6.5 g of a colorless oily liquid, which was used directly in the next step.

[0188] b) 4-chloro-8-(2,4-dimethoxybenzyl)-5,8-dihydropteridin-7(6H)-one To a solution of ethyl 2-((4,6-dichloropyrimidin-5-yl)amino)acetate (5 g) in isopropanol (150 mL) at 20° C., 2,4-dimethoxybenzylamine (3.67 g) was added, followed by triethylamine (4.45 g). The reaction mixture was stirred at 80° C. for 18 h. After the reaction was complete, the reaction solution was filtered, and the filter cake was washed with ethanol and dried under vacuum to give 5.0 g of an off-white solid, which was used directly in the next step.

[0189] c) 4-chloro-8-(2,4-dimethoxybenzyl)-5-methyl-5,8-dihydropteridin-7(6H)-one Methyl iodide (1.68 g) was added to a solution (30 mL) of 4-chloro-8-(2,4-dimethoxybenzyl)-5,6-dihydropteridin-7(8H)-one (3.3 g) dissolved in N,N-dimethylformamide at 0°C, and the reaction mixture was stirred for 20 min. Sodium hydride (0.47 g) was added while maintaining the temperature at 0°C. The reaction mixture was then stirred at 0°C for 3 h. After the reaction was completed, the reaction solution was poured into water (100 mL) and extracted with ethyl acetate (100 mL). The organic phase was washed with saturated saline (100 mL*3), dried over anhydrous sodium sulfate, and the solvent was removed by distillation under reduced pressure. The product was purified by silica gel column chromatography (petroleum ether:ethyl acetate = 5:1, volume ratio) to obtain 1.5 g of a white solid as the product, which was used directly in the next step.

[0190] d) 4-Chloro-5-methyl-5,8-dihydropteridin-7(6H)-one Trifluoroacetic acid (20 mL) was added to 4-chloro-8-(2,4-dimethoxybenzyl)-5-methyl-5,6-dihydropteridin-7(8H)-one (5 g) at 20° C. The reaction mixture was stirred at 60° C. for 16 h. After the reaction was complete, the solvent was removed by distillation under reduced pressure to give a near purple solid (1.0 g). LC-MS (ESI) m / z: 199.1 (M+H). 1H NMR (300MHz, DMSO-d6) δ (ppm) 11.60 (s, 1H), 8.35 (s, 1H), 3.77 (s, 2H), 2.85 (s, 3H).

[0191] e) t-Butyl 4-(5-methyl-7-oxo-5,6,7,8-tetrahydropteridin-4-yl)piperazine-1-carboxylate At 20°C, t-butyl piperazine-1-carboxylate (0.85g) and 4-dimethylaminopyridine (0.93g) were added to a solution (5mL) of 4-chloro-5-methyl-5,8-dihydropteridin-7(6H)-one (0.3g) in N-methylpyrrolidone. The reaction mixture was stirred at 150°C for 3h. After the reaction was complete, the reaction solution was poured into water (100mL) and extracted with ethyl acetate (100mL). The organic phase was washed with saturated saline (100mL*3), dried over anhydrous sodium sulfate, and the solvent was removed by distillation under reduced pressure. The product was purified by chromatography on silica gel plates (petroleum ether:ethyl acetate = 1:1, volume ratio) to obtain 0.27g of a white solid, which was used directly in the next step.

[0192] f) 5-Methyl-4-(piperazin-1-yl)-5,8-dihydropteridin-7(6H)-one Hydrogen chloride / 1,4-dioxane solution (4.0 M, 3 mL) was added to t-butyl 4-(5-methyl-7-oxo-5,6,7,8-tetrahydropteridin-4-yl)piperazine-1-carboxylate (0.1 g) at 20° C., and the reaction mixture was stirred for 3 hours at 20° C. After complete reaction, the solvent was removed by distillation under reduced pressure to obtain an off-white solid (0.1 g).

[0193] g) (S) (2-(4-chlorophenyl)-3-(4-(5-methyl-7-oxo-5,6,7,8-tetrahydropteridin-4-yl)-piperazin-1-yl)-3-oxopropyl)(isopropyl)carbamate t-butyl ester To 5-methyl-4-(piperazin-1-yl)-5,6-dihydropteridin-7(8H)-one (0.1 g) dissolved in N,N-dimethylformamide (10 mL) at 20° C., (S)-3-((t-butoxycarbonyl)(isopropyl)amino)-2-(4-chlorophenyl)propionic acid (0.17 g) and diisopropylethylamine (0.16 g) were added, the reaction mixture was stirred for 2 min, and 2-(7-benzotriazole oxide)-N,N,N',N'-tetramethyluronium hexafluorophosphate (0.23 g) was added at 20° C., and the reaction mixture was stirred for 1 h at 20° C. After the reaction was completed, the reaction mixture was poured into water (100 mL) and extracted with ethyl acetate (100 mL). The organic phase was washed with saturated saline (100mL*3), dried over anhydrous sodium sulfate, and the solvent was removed by distillation under reduced pressure. The product was purified by chromatography on a silica gel plate (petroleum ether:ethyl acetate=1:1.5, volume ratio) to obtain 0.08g of a white solid as the product. LC-MS(ESI)m / z:572(M+H).

[0194] h) (S)-4-(4-(2-(4-chlorophenyl)-3-(isopropylamino)propionyl)piperazin-1-yl)-5-methyl-5,8-dihydropteridin-7(6H)-one hydrochloride Hydrogen chloride / 1,4-dioxane solution (4.0 mL, 3 mL) was added to (S)-(2-(4-chlorophenyl)-3-(4-(5-methyl-7-oxo-5,6,7,8-tetrahydropteridin-4-yl)-piperazin-1-yl)-3-oxopropyl)(isopropyl) t-butyl carbamate (0.08 g) at 20° C., and the reaction mixture was stirred at 20° C. for 3 h. After complete reaction, the reaction solution was distilled under reduced pressure to remove the solvent, and the solid was isolated by high-performance preparative liquid chromatography to obtain 0.02 g of an off-white solid. LC-MS (ESI) m / z: 472 (M+H). 1H NMR(300MHz,DMSO-d6)δ(ppm)10.94(s,1H),8.12(s,1H),7.46-7.51(m,2H),7.37-7.43(m,2H),4.68-4.74(m,1H),3.81-3.91(m,2 H),3.64-3.74(m,2H),3.59-3.63(m,2H),3.52-3.59(m,4H),3.25-3.34(m,2H),2.95-3.03(m,1H),2.48(s,3H),1.20-1.28(m,6H).

[0195] Example 21 Formic acid salt of 4-(8-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)-3,8-diazabicyclo[3.2.1]octan-3-yl)-5-methyl-5,8-dihydropteridin-7(6H)-one [ka] The target compound was prepared in the same manner as in Example 20, except that t-butyl 3,8-diazabicyclo[3.2.1]octane-8-carboxylate was used instead of t-butyl piperazine-1-carboxylate. High-speed preparative liquid chromatography conditions: Column: Aglient 5 μm prep-C18 50 × 21.2 mm, Mobile phase A: water (containing 0.1% formic acid), Mobile phase B: acetonitrile. Gradient: Time 0-10 min, B phase 5-75% (volume ratio), RT = 3.4 min. LC-MS (ESI) m / z: 498 (M + H). 1 H NMR(400MHz,DMSO-d6)δ(ppm)10.89(s,1H),8.27(s,1H),8.10(d,J=25.6Hz,1H),7.31-7.48(m,4H),4.48-4.68(m,3H),4.09-4 .30(m,2H),3.50-3.60(m,3H),3.11-3.24(m,2H),2.62-2.84(m,3H),2.37-2.47(m,3H),1.49-1.88(m,4H),0.89-1.02(m,6H).

[0196] Example 22 4-((S)-4-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)-3-methylpiperazin-1-yl)-5-methyl-5,8-dihydropteridin-7(6H)-one [ka] The procedure was the same as that described in Example 20, except that (S)-1-N-Boc-2-methylpiperazine was used instead of t-butyl piperazine-1-carboxylate. The final product was adjusted to pH=9 with 10% sodium hydroxide solution at 0°C, extracted with dichloromethane (10mL x 3), washed with saturated saline (15mL), dried over anhydrous sodium sulfate, and the organic phase was removed by distillation under reduced pressure to give 70mg of a white solid. LC-MS (ESI) m / z: 486 (M+H). 1 H NMR(400MHz,DMSO-d6)δ(ppm)10.88(s,1H),8.09(d,J=5.6Hz,1H),7.35-7.42(m,3H),7. 27(d,J=8.4Hz,1H),4.55-4.74(m,3H),4.44-4.53(m,1H),4.31-4.39(m,1H),4.16-4.26( m,1H),4.00-4.10(m,1H),3.87-3.96(m,1H),3.04-3.16(m,2H),2.87-2.96(m,1H),2.77- 2.85 (m, 1H), 2.56-2.63 (m, 2H), 2.42-2.47 (m, 3H), 1.18-1.29 (m, 3H), 0.90-0.96 (m, 6H).

[0197] Scheme K: [ka] Reagents: a) aqueous ammonia solution (25%-28% wt), tetrahydrofuran, b) acetonitrile, t-butyl piperazine-1-carboxylate, N,N-diisopropylethylamine, c) sodium borohydride, methanol, saturated aqueous ammonium chloride solution, d) trichloromethyl carbonate, N,N-diisopropylethylamine, tetrahydrofuran, e) dichloromethane, hydrogen chloride / 1,4-dioxane solution (4.0M), f) (S)-3-((t-butoxycarbonyl)(isopropyl)amino)-2-(4-chlorophenyl)propionic acid, N,N-diisopropylethylamine, N,N-dimethylformamide, 2-(7-benzotriazole oxide)-N,N,N',N'-tetramethyluronium hexafluorophosphate, g) dichloromethane, hydrogen chloride / 1,4-dioxane solution (4.0M).

[0198] Example 23 Formate salt of 5-(4-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)piperazin-1-yl)-4-methyl-1,4-dihydro-2H-pyrimido[4,5-d][1,3]oxazin-2-one [ka] a) 1-(4-amino-6-chloropyrimidin-5-yl)ethanone At 20°C, 1-(4,6-dichloropyrimidin-5-yl)ethanone (2.5 g) was dissolved in tetrahydrofuran (15 mL), and aqueous ammonia (9 g) was added. The reaction solution was stirred at 20°C for 5 hours, and then concentrated, diluted with a small amount of water, and suction filtered to obtain a white solid. After drying in vacuum, 2 g of a white solid was obtained, which was used directly in the next step.

[0199] b) t-Butyl 4-(5-acetyl-6-aminopyrimidin-4-yl)piperazine-1-carboxylate At 20° C., 1-(4-amino-6-chloropyrimidin-5-yl)ethanone (2 g) and N,N-diisopropylethylamine (3 g) were dissolved in acetonitrile (20 mL), and 1-(4-amino-6-chloropyrimidin-5-yl)ethanone was added, and the reaction solution was stirred at 40° C. for 5 hours. After the reaction was completed, the solvent was removed by distillation under reduced pressure to obtain a crude product, which was separated and purified by silica gel column chromatography (ethyl acetate:petroleum ether=1:1, volume ratio) to obtain 3.2 g of a pale yellow solid, which was used directly in the next step.

[0200] c) t-Butyl 4-(6-amino-5-(1-hydroxyethyl)pyrimidin-4-yl)piperazine-1-carboxylate At 20°C, t-butyl 4-(5-acetyl-6-aminopyrimidin-4-yl)piperazine-1-carboxylate (1.5 g) was dissolved in methanol (15 mL), cooled to -10°C, and sodium borohydride (1 g) was added in portions. After the addition was completed, the reaction solution was gradually heated to 20°C and stirred for 3 hours. After the reaction was complete, the reaction solution was quenched with saturated aqueous ammonium chloride solution. Then, the reaction solution was concentrated and pulped with ethyl acetate (20 mL*2). The mother liquor was concentrated to obtain an oily crude product. The crude product was separated and purified by silica gel column chromatography (dichloromethane:methanol = 1:30, volume ratio), and 400 mg of a white oily product was obtained. LC-MS (ESI): m / z = 324 [M + H].

[0201] d) t-Butyl 4-(4-methyl-2-oxo-1,4-dihydro-2H-pyrimido[4,5-d][1,3]oxazin-5-yl)piperazine-1-carboxylate At 20°C, 4-(6-amino-5-(1-hydroxyethyl)pyrimidin-4-yl)piperazine-1-carboxylate t-butyl (300mg), N,N-diisopropylethylamine (282mg) were dissolved in tetrahydrofuran (3mL), and the temperature was lowered to -5°C, trichloromethyl carbonate was slowly added, and the mixture was stirred at -5°C for 0.5 hours. Then, the mixture was slowly heated to 18°C ​​and stirred for 1.5 hours. After the reaction was completed, the reaction was quenched with sodium bicarbonate water, extracted with ethyl acetate (10mL*3), and the organic phases were combined, dried over anhydrous sodium sulfate, filtered, and concentrated to obtain an oily crude product. The crude product was separated and purified by silica gel column chromatography (petroleum ether:ethyl acetate=1:1, volume ratio) to obtain 108mg of a white solid. LC-MS(ESI)m / z=350[M+H].

[0202] e) 4-Methyl-5-(piperazin-1-yl)-1,4-dihydro-2H-pyrimidido[4,5-d][1,3]oxazin-2-one hydrochloride At 20°C, 4-(4-methyl-2-oxo-1,4-dihydro-2H-pyrimido[4,5-d][1,3]oxazin-5-yl)piperazine-1-carboxylate t-butyl (100 mg) was dissolved in dichloromethane (3 mL), and hydrogen chloride / 1,4-dioxane solution (4.0 M, 3 mL) was added under stirring. The reaction solution was stirred at 20°C for 1 hour, and a white solid precipitated. The solvent was removed from the reaction solution by distillation under reduced pressure, and 80 mg of the target product was obtained as a white solid, which was used as it was in the next step.

[0203] f) (2S)-2-(4-chlorophenyl)-3-(4-(4-methyl-2-oxo-1,4-dihydro-2H-pyrimido[4,5-d][1,3]oxazin-5-yl)piperazin-1-yl)-3-oxopropyl)(isopropyl)carbamate t-butyl At 20°C, 4-methyl-5-(piperazin-1-yl)-1H-pyrimidido[4,5-d][1,3]oxazin-2(4H)-one (80mg), (S)-3-((t-butoxycarbonyl)(isopropyl)amino)-2-(4-chlorophenyl)propionic acid (100mg), N,N-diisopropylethylamine (113mg) and 2-(7-benzotriazole oxide)-N,N,N',N'-tetramethyluronium hexafluorophosphate (167mg) were dissolved in anhydrous N,N-dimethylformamide (2.5mL), and the reaction solution was stirred at 20°C for 12 hours. After complete reaction, the reaction solution was poured into ethyl acetate (20mL), washed twice with water and once with aqueous sodium chloride solution, and the organic phase was concentrated to obtain a crude product. The crude product was separated and purified by silica gel column chromatography (petroleum ether:ethyl acetate = 1:1, volume ratio) to obtain 130 mg of a white solid. LC-MS (ESI) m / z = 573 [M + H].

[0204] g) 5-(4-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)piperazin-1-yl)-4-methyl-1,4-dihydro-2H-pyrimido[4,5-d][1,3]oxazin-2-one formate salt At 20°C, (2S)-2-(4-chlorophenyl)-3-(4-(4-methyl-2-oxo-1,4-dihydro-1H-pyrimido[4,5-d][1,3]oxazin-5-yl)piperazin-1-yl)-3-oxopropyl)(isopropyl)carbamate t-butyl (130mg) was dissolved in dichloromethane (2mL), and hydrogen chloride / 1,4-dioxane solution (4.0mL, 2mL) was added under stirring, and the reaction solution was stirred at 20°C for 2 hours. After the reaction was completed, the reaction solution was cooled to 0°C, and 10% sodium hydroxide was added to adjust the pH to 9, extracted with dichloromethane (10mL x 3), washed with saturated saline (15mL), dried over anhydrous sodium sulfate, and the organic phase was removed by distillation under reduced pressure. The remaining product was isolated and purified by high-performance preparative liquid chromatography to obtain 32mg of a white solid. Preparation conditions: Column: Aglient 5 μm prep-C18 50 × 21.2 mm, Mobile phase A: water (containing 0.1% formic acid), Mobile phase B: acetonitrile, Gradient: Time 0-10 min, B phase 5-45% (volume ratio), RT = 3.7 min. LC-MS (ESI): m / z = 473 [M + H]. 1 H NMR(400MHz,DMSO-d6)δ(ppm)8.22-8.31(m,2H),7.37-7.45(m,2H),7.28-7.36(m,2H),5.68-5.81(m,1H),4.18-4.28(m,1H),3.4 1-3.78(m,8H),3.03-3.26(m,3H),2.89-3.01(m,1H),2.74-2.83(m,1H).2.64-2.72(m,1H).1.33-2.49(m,3H).0.89-1.04(m,6H).

[0205] Isomer separation: The above-mentioned title compound was optically resolved by supercritical fluid chromatography. Equipment and conditions for optical resolution: Waters SFC200, column: Daicel Chiralcel AD, 250×30 mm ID, 5 μm, mobile phase: A is CO2, B is ethanol (0.1% NH3H2O), A:B=65:35 (volume ratio), flow rate 60 mL / min, column temperature 38°C.

[0206] Isomer 1: [ka] LCMS(ESI): m / z=473(M+H). 1 H NMR(400MHz,DMSO-d6)δ8.28(s,1H),7.47-7.23(m,4H),5.76(d,J=6.3Hz,1H),4.15(s,1H),3.75-3.44(m, 6H),3.28-3.20(m,2H),3.15-3.04(m,2H),2.74-2.58(m,2H),1.41(d,J=6.2Hz,3H),0.93(t,J=6.9Hz,6H). Isomer 2: [ka] LCMS(ESI):M / Z=473(M+H). 1 H NMR(400MHz,DMSO-d6)δ10.85(s,1H),8.29(s,1H),7.43(dd,J=42.1,8.2Hz,4H),5.75(q,J=6.2Hz,1H),4.76-4. 54(m,1H),3.79-3.39(m,8H),3.32-3.20(m,2H),3.10-2.85(m,2H),1.43(d,J=6.4Hz,3H),1.25(t,J=5.6Hz,6H). Example 24 [ka] The preparation was carried out in the same manner as described in Scheme F, except that t-butyl 3,8-diazabicyclo[3.2.1]octane-8-carboxylate was used instead of t-butyl piperazine-1-carboxylate. The final product was separated by supercritical fluid chromatography to give isomer 1 and isomer 2. Equipment and conditions for optical resolution: waters SFC200, column: Daicel Chiralcel OD, 250×30 mm ID, 5 μm, mobile phase: A is CO2, B is ethanol (0.1% NH3H2O), A:B=70:30 (volume ratio), flow rate 60 mL / min, column temperature 38 °C.

[0207] Isomer 1: LCMS (ESI) m / z: 551 (M+H). 1 HNMR(300MHz,DMSO-d6)δ(ppm):1.28-1.11(m,6H),2.10-1.47(m,6H),3.18-2.75( m,7H),4.28-3.65(m,5H),4.85-4.83(m,1H),7.41-7.25(m,4H),8.42-8.33(m,1H). Isomer 2: LCMS (ESI) m / z: 551 (M+H). 1 HNMR (300MHz, DMSO-d6) δ (ppm): 2.28-1.20 (m, 13H), 3.43-2.89 (m, 6H), 4.37-3.71 (m, 5H), 4.86-4.81 (m, 1H), 7.39-7.27 (m, 4H), 8.36 (s, 1H).

[0208] Example 25 [ka] The preparation was carried out in the same manner as described in Scheme F, except that t-butyl 3,8-diazabicyclo[3.2.1]octane-3-carboxylate was used instead of t-butyl piperazine-1-carboxylate. The final product was separated by supercritical fluid chromatography to give isomer 1 and isomer 2. Equipment and conditions for optical resolution: waters SFC200, column: Daicel Chiralcel OZ, 250×30 mm ID, 5 μm, mobile phase: A is CO2 and B is ethanol (0.1% NH3H2O), A:B=60:40 (volume ratio), flow rate 60 mL / min, column temperature 38°C.

[0209] Isomer 1: LCMS(ESI)m / z:551(M+H). 1HNMR(400MHz,DMSO-d6)δ(ppm):0.98-0.92(m,6H),1.96-1.57(m,3H),2.87-2.59(m,5H),3.29-3.12(m,2H) ,3.63-3.42(m,2H),3.99(s,1H),4.67-4.24(m,5H),7.46-7.27(m,4H),8.30(d,J=3.6Hz,1H),10.84(s,1H).

[0210] Isomer 2: LCMS(ESI)m / z:551(M+H). 1 HNMR(400MHz,DMSO-d6)δ(ppm):0.98-0.89(m,6H),2.03-1.54(m,4H),2.76-2.57(m,4H),3.27-2.95(m,4H ),3.70-3.67(m,1H),3.99-3.95(m,1H),4.67-4.20(m,4H),7.45-7.27(m,4H),8.28(s,1H),10.83(s,1H).

[0211] Example 26 [ka] Prepared similarly to the method described in Scheme J, except t-butyl 3,8-diazabicyclo[3.2.1]octane-3-carboxylate was used instead of t-butyl piperazine-1-carboxylate. LCMS (ESI): m / z=498 (M+H). 1 H NMR(400 MHz,DMSO-d6)δ10.86(s,1H),8.06-8.15(m,1H),7.23-7.47(m,4H),4.85-5.09(m,2H ),4.11-4.31(m,2H),3.92-4.04(m,1H),3.54-3.62(m,2H),3.05-3.17(m,1H),2.84-2 .95(m,1H),2.72-2.82(m,1H),2.55-2.63(m,1H),2.41(s,3H),1.91(s,1H),1.61-1.7 7(m,1H), 1.40-1.55(m,1H), 1.22-1.33(m,1H), 0.84-1.01(m,6H), 0.40-0.53(m,1H). Example 27 [ka] This was prepared in the same manner as described in Scheme J, except that t-butyl 2,5-diazabicyclo[4.1.0]heptane-2-carboxylate was used instead of t-butyl piperazine-1-carboxylate. The final product was separated by supercritical fluid chromatography to give isomer 1 and isomer 2. Equipment and conditions for optical resolution: Waters SFC200, column: Daicel Chiralcel OZ, 250×30 mm ID, 5 μm, mobile phase: A is CO2 and B is ethanol (0.1% NH3H2O), A:B=60:40 (volume ratio), flow rate 60 mL / min, column temperature 38 °C.

[0212] Ultra-high performance convergence chromatography conditions: Column: Daicel Chiralcel AD, 2.1 × 150 mm ID, 3 μm, Mobile phase A: CO2, Mobile phase B: Ethanol (0.1% DEA), Gradient: Time 0-8 min, B phase 5-40% (volume ratio), Flow rate: 1 mL / min, Column temperature 40 °C. Isomer 1: RT = 4.0 min, Isomer 2: RT = 4.8 min.

[0213] Isomer 1: LCMS (ESI): m / z=484(M+H). 1 H NMR(400MHz,DMSO-d6)δ10.97(s,1H),8.17-8.25(m,1H),7.38-7.52(m,4H),4.44-4.51(m,1H),4.20-4.38(m,1H),3.65-3.78(m 1H),3.50-3.63(m,4H),3.13-3.33(m,3H),2.69-2.86(m,2H),2.49-2. 58(m,3H),1.44-1.51(m,1H),0.93-1.06(m,7H),0.51(d,J=5.6Hz,1H).

[0214] Isomer 2: LCMS (ESI): m / z=484(M+H). 1H NMR(400MHz,DMSO-d6)δ10.89(s,1H),7.99-8.19(m,1H),7.16-7.47(m,4H),4.07-4.36(m,2H),3.56-3.69(m,2H),3.39-3.54(m,3H),3 .03-3.25(m,3H),2.71-2.79(m,1H),2.57-2.69(m,2H),2.27-2.32(m,3H),1.23-1.35(m,1H),0.80-1.02(m,6H),0.66(q,J=4.9Hz,1H).

[0215] Example 28 [ka] Prepared similarly to the method described in Scheme J, except ethyl iodide was used instead of methyl iodide and t-butyl 3,8-diazabicyclo[3.2.1]octane-8-carboxylate was used instead of t-butyl piperazine-1-carboxylate. LCMS (ESI): m / z=512 (M+H). 1 H NMR(400MHz,DMSO-d6)δ10.88(s,1H),8.15(d,J=26.0Hz,1H),7.54-7.39(m,4 H),4.71-4.52(m,4H),4.26-4.19(m,1H),4.15-4.06(m,1H),3.75-3.57(m,2H ),3.29-3.11(m,2H),3.01-2.93(m,1H),2.85-2.78(m,1H),2.72-2.64(m,4H) ,2.09-1.98(m,1H),1.96-1.86(m,1H),1.88-1.59(m,3H),1.04-0.87(m,7H).

[0216] Example 29 [ka] Prepared similarly to the method described in Scheme J except bromoacetonitrile was used instead of methyl iodide.

[0217] LCMS(ESI):m / z:523(M+H). 1H NMR(400MHz,DMSO-d6)δ11.02(s,1H),8.16(d,J=22.9Hz,1H),7.38(dd,J=11.8,2.4Hz,4H),4.61-4.44(m,2H),4.34-4.08(m ,3H),4.05-3.94(m,2H),3.85-3.80(m,2H),3.23-3.01(m,2H),2.77-2.66(m,2H),1.90-1.53(m,4H),0.94(t,J=5.6Hz,6H).

[0218] Example 30 [ka] Prepared similarly to the method described in Scheme J except 1-fluoro-2-iodoethane was used instead of methyl iodide.

[0219] LCMS(ESI)m / z:530(M+H) 1 H NMR(400MHz,DMSO-d6)δ8.28(d,J=5.3Hz,1H),8.05-8.15(m,1H),7.33-7.49(m,4H),4.22-4.70(m,8H),3.48 -3.74(m,1H),3.21-3.43(m,2H),2.88-3.16(m,3H),2.74-2.86(m,1H),1.44-2.02(m,4H),0.97-1.11(m,6H).

[0220] Scheme J-1: [ka] Reaction conditions: a) dimethyl t-butyl(2-iodoethoxy)silane, sodium hydride, N,N-dimethylformamide, b) t-butyl 3,8-diazabicyclo[3.2.1]octane-8-carboxylate, 4-dimethylaminopyridine, N-methylpyrrolidone, c) hydrogen chloride in dioxane, d) (S)-3-((t-butoxycarbonyl)(isopropyl)amino)-2-(4-chlorophenyl)propionic acid, diisopropylethylamine, 2-(7-benzotriazole oxide)-N,N,N',N'-tetramethyluronium hexafluorophosphate, N,N-dimethylformamide, e) trifluoroacetic acid, dichloromethane.

[0221] Example 31 [ka] a) 5-(2-((t-butyldimethylsilyl)oxyethyl)-4-chloro-8-(2,4-dimethoxybenzyl)-5,8-dihydropteridin-7(6H)-one Sodium hydride (0.53 g) was added to 4-chloro-8-(2,4-dimethoxybenzyl)-5,8-dihydropteridin-7(6H)-one (2.2 g) dissolved in N,N-dimethylformamide (30 mL) at 0°C. The reaction mixture was stirred for 20 minutes. Dimethyl t-butyl(2-iodoethoxy)silane (2.82 g) was added at 0°C. The reaction mixture was stirred at 0°C for 3 h. The reaction mixture was poured into water (100 mL) and extracted with ethyl acetate (100 mL). The organic phase was washed with saturated saline (100 mL*3), dried over anhydrous sodium sulfate, and dried in an evaporator. The product was purified by silica gel column chromatography (petroleum ether:ethyl acetate=5:1) to obtain 0.8 g of a white solid as the product.

[0222] b) 5-(2-((t-butyldimethylsilyl)oxyethyl)-4-(8-t-butyloxycarbonyl-3,8-diazabicyclo[3,2,1]octan-3-yl)-8-(2,4-dimethoxybenzyl)-5,8-dihydropteridin-7(6H)-one To the product of step a) (0.2 g) dissolved in N-methylpyrrolidone (5 mL) at 20°C, 3,8-diazabicyclo[3.2.1]octane-8-carboxylate t-butyl (0.85 g) and 4-dimethylaminopyridine (0.17 g) were added. The reaction mixture was stirred at 150°C for 3 h. The reaction mixture was poured into water (100 mL) and extracted with ethyl acetate (100 mL). The organic phase was washed with saturated saline (100 mL*3), dried over anhydrous sodium sulfate, and dried in an evaporator. The product was purified by chromatography on silica gel plates (petroleum ether:ethyl acetate=1:1) to obtain 0.08 g of a white solid as the product.

[0223] c) 4-(3,8-diazabicyclo[3.2.1]octan-3-yl)-8-(2,4-dimethoxybenzyl)-5-(2-hydroxyethyl)-5,8-dihydropteridin-7(6H)-one To the product of step b) (0.08 g) was added a solution of hydrogen chloride in dioxane (3 mL) at 20° C. The reaction mixture was stirred at 20° C. for 3 h. The reaction mixture was evaporated to dryness to give an off-white solid (0.06 g).

[0224] d) t-Butyl ((S)-2-(4-chlorophenyl)-3-3-(8-(2,4-dimethoxybenzyl)-5-(2-hydroxyethyl)-7-oxo-5,6,7,8-tetrahydropteridin-4-yl)-3,8-diazabicyclo[3.2.1]octane-8-carbamoyl)-3-oxopropyl(isopropyl) ester To the product of step c) (0.06 g) dissolved in N,N-dimethylformamide (10 mL) at 20°C, (S)-3-((t-butoxycarbonyl)(isopropyl)amino)-2-(4-chlorophenyl)propionic acid (0.04 g) and diisopropylethylamine (0.03 g) were added. The reaction mixture was stirred for 2 minutes. 2-(7-benzotriazole oxide)-N,N,N',N'-tetramethyluronium hexafluorophosphate (0.04 g) was added at 20°C, and the reaction mixture was stirred at 20°C for 1 h. The reaction mixture was poured into water (100 mL) and extracted with ethyl acetate (100 mL). The organic phase was washed with saturated saline (100 mL*3), dried over anhydrous sodium sulfate, and dried in an evaporator. The product was purified by chromatography on silica gel plate (petroleum ether:ethyl acetate=1:2) to give 0.04 g of a white solid as the product.

[0225] e) 4-(-8-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)-3,8-diazabicyclo[3.2.1]octan-3-yl)-5-(2-hydroxyethyl)-5,6-dihydropteridin-7(8H)-one To the product of step d) (40 mg) at 20 °C was added trifluoroacetic acid (3 mL). The reaction mixture was stirred at 60 °C for 3 h. The reaction was evaporated to dryness, NaHCO3 was added, stirred for 20 min, and filtered to give an off-white solid (10 mg). LCMS (ESI) m / z: 528 (M+H). 1 H NMR:(400MHz,DMSO-d6)δ10.78(d,J=19.6Hz,1H),8.07(d,J=29.4Hz,1H),7.30-7.51(m,4H),4.61-4.70(m,1H),4.384-4.54(m,1H),4.26-4.3 6(m,1H),4.04-4.25(m,1H),3.49-3.78(m,6H),2.97-3.23(m,4H),2.82 -2.91(m,1H),2.63-2.80(m,1H),1.62-2.02(m,4H),1.27-1.17(m,8H).

[0226] Example 32 [ka] This was prepared in the same manner as described in Scheme K, except that t-butyl 3,8-diazabicyclo[3.2.1]octane-8-carboxylate was used instead of t-butyl piperazine-1-carboxylate. The final product was separated by supercritical fluid chromatography to give isomer 1 and isomer 2. Equipment and conditions for optical resolution: waters SFC200, column: Daicel Chiralcel OZ, 250×30 mm ID, 5 μm, mobile phase: A is CO2 and B is isopropanol (0.1% NH3H2O), A:B=60:40 (volume ratio), flow rate 60 mL / min, column temperature 38 °C.

[0227] Ultra-high performance convergence chromatography conditions: Column: Daicel Chiralcel AD, 2.1 × 150 mm ID, 3 μm, Mobile phase A: CO2, Mobile phase B: Isopropanol (0.1% DEA), Gradient: Time 0-8 min, B phase 5-40% (volume ratio), Flow rate: 1 mL / min, Column temperature 40 °C. Isomer 1: RT = 4.3 min, Isomer 2: RT = 4.5 min.

[0228] Isomer 1: LCMS(ESI)m / z:499(M+H). 1 H NMR(400MHz,DMSO-d6)δ8.23(d,J=11.7Hz,1H),7.50-7.21(m,4H),5.79-5.5 9(m,1H),4.64-4.47(m,2H),4.08-3.94(m,2H),3.70-3.40(m,2H),3.31-3.2 0(m,2H),3.16-3.06(m,1H),2.82(d,J=9.2Hz,0.5H),2.75-2.62(m,2H),2.1 7(d,J=8.7Hz,0.5H)1.96-1.49(m,4H),1.46-1.28(m,3H),0.98-0.87(m,6H).

[0229] Isomer 2: LCMS(ESI)m / z:499(M+H). 1H NMR(400MHz,DMSO-d6)δ8.24(d,J=16.4Hz,1H),7.53-7.21(m,4H),5.81-5.65(m,1H), 4.73-4.60(m,2H),4.46(d,J=4.9Hz,0.5H),4.19(d,J=5.1Hz,0.5H),4.03(d,J=9.3Hz ,0.5H),3.76(d,J=9.3Hz,0.5H),3.70-3.49(m,3H),3.21-3.16(m,1H),3.06-2.92(m, 2H), 2.11-1.86(m, 1.5H), 1.84-1.52(m, 4H), 1.48-1.33(m, 3.5H), 1.30-1.22(m, 6H).

[0230] Example 33 [ka] The method was similar to that described in Scheme K, except that t-butyl 3,8-diazabicyclo[3.2.1]octane-3-carboxylate was used instead of t-butyl piperazine-1-carboxylate. The final product was separated by supercritical fluid chromatography to give isomer 1 and isomer 2. Equipment and conditions for optical resolution: Waters SFC200, column: Daicel Chiralcel OZ, 250×30 mm ID, 5 μm, mobile phase: A is CO2 and B is ethanol (0.1% NH3H2O), A:B=60:40 (volume ratio), flow rate 60 mL / min, column temperature 38 °C.

[0231] Ultra-high performance convergence chromatography conditions: Column: Daicel Chiralcel AD, 2.1 × 150 mm ID, 3 μm, Mobile phase A: CO2, Mobile phase B: Ethanol (0.1% DEA), Gradient: Time 0-8 min, B phase 5-40% (volume ratio), Flow rate: 1 mL / min, Column temperature 40 °C. Isomer 1: RT = 4.6 min, Isomer 2: RT = 5.0 min.

[0232] Isomer 1: LCMS(ESI)m / z:499(M+H). 1H NMR(400MHz,DMSO-d6)δ10.87(s,1H),8.23(d,J=11.7Hz,1H),7.50-7.21(m,4H),5.80- 5.76(m,1H),4.68-4.64(m,1H),4.44-4.39(m,1H),4.33-4.14(m,2H),4.07-3.88(m,2H ),3.66-3.62(m,1H),3.16-2.88(m,2H),2.79-2.55(m,4H),2.01-1.77(m,1H),1.72-1. 49(m,2H),1.48-1.36(m,2H),1.31-1.18(m,1H),1.04-0.85(m,4H),0.54-0.44(m,1H). Isomer 2: LCMS(ESI)m / z:499(M+H). 1 H NMR(400MHz,DMSO-d6)δ10.69(s,1H),8.24(d,J=3.8Hz,1H),7.53-7.21(m,4 H),5.80-5.76(m,1H),4.64-4.48(m,1H),4.43-4.42(m,1H),4.27-4.18(m,1H) ),4.02-3.92(m,1H),3.68-3.60(m,1H),3.21-3.02(m,1H),2.95-2.64(m,4H) ,2.02-1.70(m,2H),1.60-1.22(m,5H),1.04-0.83(m,6H),0.43-0.40(m,1H).

[0233] Example 34 [ka] a) 1-(4-amino-6-chloropyrimidin-5-yl)ethanone (compound 34-1) At 20°C, 1-(4,6-dichloropyrimidin-5-yl)ethanone (2.5 g) was dissolved in tetrahydrofuran (15 mL), and aqueous ammonia (9 g) was added. The reaction solution was stirred at 20°C for 5 hours, and then concentrated, diluted with a small amount of water, and suction filtered to obtain a white solid. After drying in vacuum, 2 g of a white solid was obtained, which was used directly in the next step.

[0234] b) 1-(4-amino-6-chloropyrimidin-5-yl)ethan-1-ol (compound 34-2) 1-(4-amino-6-chloropyrimidin-5-yl)ethanone (1.5 g) was dissolved in methanol (15 mL) at 20°C, cooled to -10°C, and sodium borohydride (1 g) was added in portions. After the addition was complete, the reaction was slowly warmed to 20°C and stirred for 3 hours. After the reaction was complete, the reaction was quenched with saturated aqueous ammonium chloride solution. The reaction was then concentrated and pulped with ethyl acetate (20 mL*2). The mother liquor was concentrated to obtain an oily crude product. The crude product was isolated by column chromatography to obtain 400 mg of a white oily product. LC-MS (ESI) m / z: 174 (M+H).

[0235] c) 5-chloro-4-methyl-1,4-dihydro-2H-pyrimidido[4,5-d][1,3]oxazin-2-one (compound 34-3) At 20°C, 1-(4-amino-6-chloropyrimidin-5-yl)ethan-1-ol (300mg), N,N-diisopropylethylamine (282mg) were dissolved in tetrahydrofuran (3mL), and the temperature was lowered to -5°C, di(trichloromethyl)carbonate (300mg) was slowly added, and the mixture was stirred at -5°C for 0.5 hours. Then, the mixture was slowly heated to 18°C ​​and stirred for 1.5 hours. After the reaction was complete, the reaction was quenched with sodium bicarbonate water, extracted with ethyl acetate (10mL*3), and the organic phases were combined, dried over anhydrous sodium sulfate, filtered, and concentrated to obtain an oily crude product. The crude product was isolated and purified by column chromatography to obtain 108mg of a white solid. LC / MS(ESI)m / z:200(M+H).

[0236] d) (S)-5-chloro-4-methyl-1,4-dihydro-2H-pyrimidin[4,5-d][1,3]oxazin-2-one (compound 34-4a) and (R)-5-chloro-4-methyl-1,4-dihydro-2H-pyrimidin[4,5-d][1,3]oxazin-2-one (compound 34-4b) Compound 34-3 was subjected to optical resolution using an SFC chiral column to obtain the desired target compounds, Compound 34-4a and Compound 34-4b.

[0237] The conditions for SFC optical resolution were as follows: equipment: waters SFC200, column: Daicel Chiralcel AD, 250×50 mm ID, 10 μm, mobile phase: A: CO2, B: methanol (0.1% NH3H2O), A:B=65:35 (volume ratio), flow rate: 150 mL / min, pressure: 100 bar, column temperature: 38°C, measurement wavelength: 220 nm, circulation time: 14 min, sample pretreatment: 10 g of sample was dissolved in 300 ml of MeOH, injection volume: 16 ml.

[0238] Work-up: The samples were concentrated at 40° C. and then lyophilized to give the title compounds 34-4a and 34-4b, respectively.

[0239] Route 1: Preparation of Isomer 1 and Isomer 4 e) 5-((S)4-methyl-2-oxo-1,4-dihydro-2H-pyrimido[4,5-d][1,3]oxazin-5-yl)-2,5-diazabicyclo[4.1.0]heptane-2-carboxylate t-butyl (compound 34-5a) Compound 34-4a (2g) and 2,5-diazabicyclo[4.1.0]heptane-2-carboxylate t-butyl (3.58g) were dissolved in anhydrous MeCN (20mL), DIEA (3.89g) was added, the reaction solution was blown out with nitrogen gas, and the reaction solution was sealed and stirred at 95°C for 6 hours. After the reaction was completed, the reaction solution was concentrated to obtain the crude product. The crude product was dissolved in DCM, washed with water, and then concentrated to obtain the crude product. The crude product was isolated and purified by column chromatography (EA:PE=1:1) to obtain 3.2g of a light brown solid.

[0240] f) (4S)-5-(2,5-diazabicyclo[4.1.0]heptan-2-yl)-4-methyl-1,4-dihydro-2H-pyrimido[4,5-d][1,3]oxazin-2-one hydrochloride (compound 34-6a) The product of step e) (3.2 g) was dissolved in HCl / i-PrOH (10 mL) and stirred at room temperature for 2 h. After the reaction was completed, it was concentrated to give the crude product which was used directly in the next step without purification.

[0241] g) Compound 34-7a The product of step f) (3.3 g), (S)-3-((t-butoxycarbonyl)(isopropyl)amino)-2-(4-chlorophenyl)propionic acid (4.9 g), HATU (6.32 g), and DIPEA (4.3 g) were dissolved in anhydrous DMF (50 mL), and the reaction solution was stirred at room temperature for 12 h. After the reaction was completed, the reaction solution was poured into 100 mL of ethyl acetate, washed with water (20 mL*3) and 10 mL of saturated saline, and the organic phase was dried and concentrated to obtain the crude product. The crude product was isolated and purified by column chromatography (PE:EA=1:1) to obtain 7.2 g of a brown solid. MS(ESI) m / z: 585 (M+H).

[0242] h) Compound 34-8a The product of step g) (7.2 g) was dissolved in MeOH (25 mL) and HCl / dioxane (70 mL) was added and the reaction was stirred at room temperature for 2 h. The reaction was then concentrated to give the crude product as a red oil, which was further dissolved in MeOH (20 mL), liberated with Na2CO3, and concentrated to give the crude product, 6 g.

[0243] i) Isomer 1 Harmonic isomer 4 Compound 34-8a was optically resolved using an SFC chiral column to give isomer 1 and isomer 4. SFC optical resolution conditions: Equipment: waters SFC200, Column: Daicel Chiralcel AD, 250×50 mm ID, 10 μm, Mobile phase: A: CO2, B: MeOH (0.1% NH3H2O), A:B=75:25, Flow rate: 70 mL / min, Pressure: 100 bar, Column temperature: 38°C, Measurement wavelength: 254 nm, Circulation time: 5 min, Sample pretreatment: 10 g of sample was dissolved in 200 ml of MeOH, Charge amount: 16 ml.

[0244] Work-up: The samples were concentrated at 40° C. and then freeze-dried to give the title compounds Isomer 1 and Isomer 4, respectively.

[0245] Route 2: Preparation of Isomer 2 and Isomer 3 Starting from compound 34-4b, isomer 2 and isomer 3 were prepared in the same manner as described in Route 1.

[0246] Isomer 1: LCMS m / z:485(M+H). 1 H NMR(400MHz,DMSO-d6)δ10.70(s,1H),8.23(s,1H),7.46(d,J=8.5Hz,2H),7.39(d,J=8.6Hz,2H),6.13(q,J=6.6Hz,1H),4.51(s,1H), 4.42-4.30(m,1H),3.53-3.45(m,1H),3.28-3.06(m,5H),3.01-2.59(m,3H),1.52-1.34(m,4H),1.08-0.97(m,6H),0.93-0.84(m,1H).

[0247] Isomer 4: LCMS m / z:485(M+H). 1 H NMR(400MHz,DMSO-d6)δ10.73(s,1H),8.23(s,1H),7.41-7.32(m,4H),6.14(q, J=8.0Hz,1H),4.40-4.36(m,1H),4.19-4.11(m,1H),3.62-3.51(m,2H),3.49-3. 35(m,1H),3.24-3.05(m,4H),2.73-2.63(m,2H),1.45(d,J=8.0Hz,1H),1.33(d ,J=8.0Hz,2H),1.12(q,J=4.0Hz,1H),0.95-0.88(m,6H),0.26(q,J=4.0Hz,1H).

[0248] Isomer 2: LCMS m / z:485(M+H). 1H NMR(400MHz,DMSO-d6)δ10.86(s,1H),8.27(s,1H),7.54-7.27(m,4H),6.32-6.18(m,1H),4.69-4.52(m,1H),4.27-3.97( m,2H),3.66-3.43(m,2H),3.29-2.92(m,6H),2.61-2.55(m,1H),1.63-1.58(m,1H),1.53-1.28(m,3H),1.28-1.12(m,6H).

[0249] Isomer 3: LCMS m / z:485(M+H). 1 H NMR(400MHz,DMSO-d6)δ10.71(s,1H),8.18(s,1H),7.46-7.39(m,1H),7.36-7.28(m,3H),6.00(q,J=6.4Hz,1H),4.53(s,1H),4.46 -4.33(m,1H),3.56-3.44(m,2H),3.26-3.09(m,5H),3.00-2.71(m,2H),1.43-1.38(m,3H),1.10-0.93(m,7H),-0.07--0.11(m,1H).

[0250] Determining configuration by single crystal diffraction: (1) Determination of the configuration of isomer 1 Preparation of single crystals: Compound isomer 1 (50.0 mg) and 3.0 ml of isopropanol were weighed and added to a 5 ml screw-top glass bottle, and stirred for 5 min until the solid was completely dissolved. 13.0 mg of oxalic acid dihydrate was weighed and placed in the above glass bottle, and a white solid gradually precipitated in the glass bottle. After stirring at room temperature for 3 h, a large amount of white solid precipitated in the glass bottle. After adding 1.5 ml of methanol and 0.2 ml of purified water to the glass bottle, the white solid gradually disappeared and the solution became transparent, and then stirred for 1 h. The solution was passed through a 0.22 μm microporous filter membrane and filtered into a 20 ml screw-top glass bottle, and the mouth of the glass bottle was covered with plastic wrap. Eight holes were made in the mouth of the bottle with a needle, and the bottle was left at room temperature for 10 days to obtain a single crystal of the oxalate salt of the isomer 1 compound. JPEG0007617005000121.jpg81166

[0251] Structure description: Single crystal X-ray diffraction and structure analysis show that the obtained single crystal is the oxalate salt of isomer 1. The asymmetric structural unit of the crystal contains two molecules of the isomer and one molecule of oxalic acid. The single molecule schematic diagram of compound isomer 1 is shown in Figure 3, and the oxalate single crystal is shown in Figure 4. The structural formula is shown below: [ka] (2) Determination of the configuration of isomer 3 Preparation of Single Crystals: Single crystals of isomer 3 oxalate were prepared according to the method described for the preparation of single crystals of isomer 1 above. Single crystal diffraction study: JPEG0007617005000123.jpg79166

[0252] Structure description: Single crystal X-ray diffraction and structure analysis show that the obtained single crystal is an oxalate hydrate of isomer 3. The asymmetric structural unit of the crystal contains two molecules of isomer 3, two molecules of oxalic acid and one molecule of water, and isomer 3 and oxalic acid form an oxalate salt. The single molecule schematic diagram of compound isomer 3 is shown in Figure 5, and the asymmetric structural unit of the oxalate salt single crystal is shown in Figure 6. The structural formula is shown below: [ka] Scheme L: [ka] Reaction conditions: a) (2,4-dimethoxyphenyl)methylamine, triethylamine, tetrahydrofuran, b) 4,6-dichloropyrimidin-5-amine, triethylamine, isopropanol, c) methyl iodide, sodium hydride, N,N-dimethylformamide, d) t-butyl piperazine-1-carboxylate, 4-dimethylaminopyridine, N-methylpyrrolidone, e) hydrogen chloride in dioxane, f) (S)-3-((t-butoxycarbonyl)(isopropyl)amino)-2-(4-chlorophenyl)propionic acid, diisopropylethylamine, 2-(7-benzotriazole oxide)-N,N,N',N'-tetramethyluronium hexafluorophosphate, N,N-dimethylformamide, g) trifluoroacetic acid.

[0253] Example 35 [ka] a) Ethyl 2-(2,4-dimethoxybenzyl)aminoacetate Triethylamine (0.6 g) and (2,4-dimethoxyphenyl)methylamine (1.0 g) were added to a THF solution (10 mL) containing ethyl bromoacetate (1.0 g) at 0°C. The reaction mixture was stirred at room temperature for 3 h. The reaction mixture was poured into water (100 mL) and extracted with ethyl acetate (100 mL). The organic phase was washed with saturated saline (100 mL*3), dried over anhydrous sodium sulfate, and dried in an evaporator. The product was purified by silica gel column chromatography (petroleum ether:ethyl acetate=3:1) to obtain 1.3 g of a white solid as the product.

[0254] b) 4-chloro-8-(2,4-dimethoxybenzyl)-7,8-dihydropteridin-6(5H)-one Triethylamine (1.56 g) and 4,6-dichloropyrimidin-5-amine (0.84 g) were added to ethyl 2-(2,4-dimethoxybenzyl)aminoacetate (1.3 g) dissolved in isopropanol (10 mL) at 0° C. The reaction mixture was stirred at 90° C. for 3 h. The reaction mixture was filtered to obtain 0.7 g of a white solid as the product.

[0255] c) 4-chloro-8-(2,4-dimethoxybenzyl)-5-methyl-7,8-dihydropteridin-6(5H)-one Sodium hydride (76.7 mg) was added to a solution (5 mL) of 4-chloro-8-(2,4-dimethoxybenzyl)-7,8-dihydropteridin-6(5H)-one (0.5 g) dissolved in N,N-dimethylformamide at 0°C. The reaction mixture was stirred for 20 minutes. Methyl iodide (255 mg) was added at 0°C. The reaction mixture was stirred at 0°C for 3 h. The reaction mixture was poured into water (100 mL) and extracted with ethyl acetate (100 mL). The organic phase was washed with saturated saline (100 mL*3), dried over anhydrous sodium sulfate, and dried in an evaporator. The product was purified by silica gel column chromatography (petroleum ether:ethyl acetate=5:1) to obtain 0.5 g of a white solid as the product.

[0256] d) 4-(8-(2,4-dimethoxybenzyl)-5-methyl-6-oxo-5,6,7,8-tetrahydropteridin-4-yl)piperazine-1-carboxylic acid ester At 20°C, 4-chloro-8-(2,4-dimethoxybenzyl)-5-methyl-7,8-dihydropteridin-6(5H)-one (0.5g) was dissolved in N-methylpyrrolidone solution (10mL), and piperazine-1-carboxylate t-butyl (0.53g) and 4-dimethylaminopyridine (0.52g) were added. The reaction mixture was stirred at 150°C for 3h. The reaction mixture was poured into water (100mL) and extracted with ethyl acetate (100mL). The organic phase was washed with saturated saline (100mL*3), dried over anhydrous sodium sulfate, and dried in an evaporator. The product was purified by chromatography on silica gel plate (petroleum ether:ethyl acetate=1:1) to obtain 0.5g of white solid as the product.

[0257] e) 8-(2,4-dimethoxybenzyl)-5-methyl-4-(piperazin-1-yl)-7,8-dihydropteridin-6(5H)-one A hydrogen chloride dioxane solution (5 mL) was added to 4-(8-(2,4-dimethoxybenzyl)-5-methyl-6-oxo-5,6,7,8-tetrahydropteridin-4-yl)piperazine-1-carboxylate (0.1 g) at 20° C. The reaction mixture was stirred at 20° C. for 3 h. The reaction solution was dried in an evaporator to obtain an off-white solid (0.1 g).

[0258] f) (S)-t-Butyl (2-(4-chlorophenyl)-3-(4-(8-(2,4-dimethoxybenzyl)-5-methyl-6-oxo-5,6,7,8-tetrahydropteridine-4-yl)-piperazin-1-yl)-3-oxopropyl) (isopropyl)carbamate (S)-3-((t-butoxycarbonyl)(isopropyl)amino)-2-(4-chlorophenyl)propionic acid (0.12 g) and diisopropylethylamine (0.10 g) were added to 8-(2,4-dimethoxybenzyl)-5-methyl-4-(piperazin-1-yl)-7,8-dihydropteridin-6(5H)-one (0.1 g) dissolved in N,N-dimethylformamide (10 mL) at 20°C. The reaction mixture was stirred for 2 minutes. 2-(7-benzotriazole oxide)-N,N,N',N'-tetramethyluronium hexafluorophosphate (0.12 g) was added at 20°C, and the reaction mixture was stirred at 20°C for 1 h. The reaction mixture was poured into water (100 mL) and extracted with ethyl acetate (100 mL). The organic phase was washed with saturated saline (100mL*3), dried over anhydrous sodium sulfate, and dried in an evaporator. The product was purified by chromatography on a silica gel plate (petroleum ether: ethyl acetate = 1:2) to obtain 0.1g of a white solid as the product.

[0259] g) (S)-4-(4-(2-(4-chlorophenyl)-3-(isopropylamino)propionyl)piperazin-1-yl)-5-methyl-7,8-dihydropteridin-6(5H)-one Trifluoroacetic acid (5 mL) was added to (S)-t-butyl(2-(4-chlorophenyl)-3-(4-(8-(2,4-dimethoxybenzyl)-5-methyl-6-oxo-5,6,7,8-tetrahydropteridin-4-yl)-piperazin-1-yl)-3-oxopropyl)(isopropyl) carbamate (100 mg) at 20° C. The reaction mixture was stirred at 60° C. for 3 h. The reaction solution was dried in an evaporator to give an off-white solid (20 mg). LCMS (ESI) m / z: 472 (M+H) 1 H NMR(400MHz,CDCl3)δ7.89(s,1H),7.22-7.29(m,2H),7.10-7.18(m,2H),4.08-4.17(m,1H),3.86(s,2H),3.32-3.45 (m,2H),3.15-3.30(m,3H),3.08(s,3H),2.82-2.93(m,2H),2.51-2.78(m,4H),1.21-1.31(m,2H),1.03-1.12(m,6H).

[0260] Scheme M: [ka] Reaction conditions: a) 2,4-dimethoxybenzylamine, N,N-diisopropylethylamine, acetonitrile, b) tetrahydrofuran, potassium carbonate, methyl 2-chloro-2-oxoacetate, c) N,N-diisopropylethylamine, ethanol, d) t-butyl 3,8-diazabicyclo[3.2.1]octane-8-carboxylate, N,N-diisopropylethylamine, acetonitrile, e) N,N-dimethylformamide, potassium carbonate, methyl iodide, f) trifluoroacetic acid, g) (S)-3-((t-butoxycarbonyl)(isopropyl)amino)-2-(4-chlorophenyl)propionic acid, N,N-diisopropylethylamine, N,N-dimethylformamide, HATU, h) dichloromethane, hydrogen chloride / isopropanol solution.

[0261] Example 36 [ka] a) 6-chloro-N-(2,4-dimethoxybenzyl)pyrimidine-4,5-diamine 4,6-Dichloropyrimidin-5-amine (2g), N,N-diisopropylethylamine (4.7g), and 2,4-dimethoxybenzylamine (2.45g) were dissolved in acetonitrile (20mL), the reaction solution was stirred at 90°C for 5 hours, and then concentrated to obtain a crude product, which was dissolved in dichloromethane and washed with a small amount of water. The organic phase was dried in an evaporator to obtain a crude product, which was isolated and purified by column chromatography (PE:EA=1:1), and concentrated to obtain the pale yellow target product (3.5g).

[0262] b) 2-((4-chloro-6-(((2,4-dimethoxybenzyl)amino)pyrimidin-5-yl)amino)-2-oxoacetate methyl 6-Chloro-N-(2,4-dimethoxybenzyl)pyrimidine-4,5-diamine (1.5g), potassium carbonate (1.1g), and 2-chloro-2-oxomethyl acetate (623mg) were dissolved in absolute ethanol (20mL), and the reaction solution was stirred at 20°C for 3 hours. The reaction solution was poured into 30mL of ethyl acetate, washed with water (10mL*2), and the organic phase was concentrated until the weight remained unchanged to obtain a crude product. The crude product was pulped and purified with a mixed solvent (PE:EA=3:1) to obtain a white solid (1.6g) as the product.

[0263] c) 4-chloro-8-(2,4-dimethoxybenzyl)-5,8-dihydropteridine-6,7-dione 2-((4-chloro-6-(((2,4-dimethoxybenzyl)amino)pyrimidin-5-yl)amino)-2-oxoacetate methyl (1g) was dissolved in absolute ethanol (10mL), N,N-diisopropylethylamine (1.02g) was added, and the reaction solution was stirred at 90℃-110℃ for 2 hours under nitrogen gas atmosphere. The reaction solution was concentrated to obtain a crude product, which was dissolved again in dichloromethane and adjusted to a weak acidity with dilute hydrochloric acid, and the salt was removed by washing with water. The organic phase was concentrated to obtain a crude product, which was pulped and purified with a mixed solvent (PE:EA=3:1) to obtain a white oily product (800mg).1 H NMR(400MHz,DMSO-d6)δ12.15(s,1H),8.47(s,1H),6.88(d,J=8.4Hz,1H),6.59(d, J=2.3Hz,1H),6.32(dd,J=8.4,2.3Hz,1H),5.21(s,2H),3.84(s,3H),3.72(s,3H).

[0264] d) t-Butyl-3-(8-(2,4-dimethoxybenzyl)-6,7-dioxo-5,6,7,8-tetrahydropteridin-4-yl)-3,8-diazabicyclo[3.2.1]octane-8-carboxylate 4-Chloro-8-(2,4-dimethoxybenzyl)-5,8-dihydropteridine-6,7-dione (250 mg), (1R,5S)-3,8-diazabicyclo[3.2.1]octane-8-carboxylate t-butyl (456 mg), and N,N-diisopropylethylamine (278 mg) were dissolved in anhydrous acetonitrile (5 mL), and the reaction solution was sealed under a nitrogen gas atmosphere and stirred at 90°C for 3 hours. The mixture was concentrated to obtain an oily crude product. The crude product was further dissolved in ethyl acetate (30 mL), adjusted to pH 3-4 with dilute hydrochloric acid, washed with water to pH 5-6, and further concentrated to obtain a crude product. The crude product was isolated and purified by column chromatography (PE:EA=1:1) to obtain 200 mg of a white solid, m / z=525 (M+H).

[0265] e) (t-Butyl-3-(8-(2,4-dimethoxybenzyl)-5-methyl-6,7-dioxo-5,6,7,8-tetrahydropteridin-4-yl)-3,8-diazabicyclo[3.2.1]octane-8-carboxylate t-Butyl-3-(8-(2,4-dimethoxybenzyl)-6,7-dioxo-5,6,7,8-tetrahydropteridin-4-yl)-3,8-diazabicyclo[3.2.1]octane-8-carboxylate (180 mg), methyl iodide (146 mg), potassium carbonate (142 mg) were dissolved in anhydrous DMF (3 mL), the reaction solution was stirred at 20 °C for 1 h, and the reaction solution was poured into 30 mL EA, washed with water (10 mL * 2) and saturated saline (10 mL), and the organic phase was concentrated to obtain the crude product. The crude product was isolated and purified by column chromatography (PE:EA = 3:1) to obtain 180 mg of a pale yellow solid.

[0266] f) 4-((1R,5S)-3,8-diazabicyclo[3.2.1]octan-3-yl)-5-methyl-5,8-dihydropteridine-6,7-dione t-Butyl-3-(8-(2,4-dimethoxybenzyl)-5-methyl-6,7-dioxo-5,6,7,8-tetrahydropteridin-4-yl)-3,8-diazabicyclo[3.2.1]octane-8-carboxylate (120 mg) was dissolved in anhydrous DCM (1 mL), TFA (2 mL) was added dropwise, and the reaction solution was stirred at room temperature for 3 hours. The reaction solution turned red, and the reaction solution was concentrated as it was to obtain a crude product. The pH was adjusted to 8-9 with sodium bicarbonate water, and the crude product was further concentrated and dissolved in THF. The salt was removed by filtration, and the remaining product was isolated by column chromatography (DCM:MeOH=10:1) to obtain 60 mg of an oily product.

[0267] g) t-Butyl ((s)-2-(4-chlorophenyl)-3-3-(5-methyl-6,7-dioxo-5,6,7,8-tetrahydropteridin-4-yl)-3,8-diazabicyclo[3.2.1]octan-8-yl)-3-oxopropylcarbamate (isopropyl) Compound 4-(3,8-diazabicyclo[3.2.1]octan-3-yl)-5-methyl-5,8-dihydropteridine-6,7-dione (60 mg), (S)-3-((t-butoxycarbonyl)(isopropyl)amino)-2-(4-chlorophenyl)propionic acid (90 mg), 2-(7-azabenzotriazole)-N,N,N',N'-tetramethyluronium hexafluorophosphate (130 mg), N,N-diisopropylethylamine (102 mg) were dissolved in anhydrous DMF (2 mL), the reaction solution was stirred at room temperature for 3 hours, and the reaction solution was poured into 20 mL of ethyl acetate, washed with water (50 mL * 2), the organic phase was dried over anhydrous sodium sulfate, and concentrated to obtain a crude product. The crude product was isolated and purified by silica gel column chromatography (DCM:MeOH = 10:1) to obtain 120 mg of an oily product.

[0268] h) 4-(8-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)-3,8-diazabicyclo[3.2.1]octan-3-yl)-5-methyl-5,8-dihydropteridin-6,7-one t-Butyl ((s)-2-(4-chlorophenyl)-3-(5-methyl-6,7-dioxo-5,6,7,8-tetrahydropteridin-4-yl)-3,8-diazabicyclo[3.2.1]octan-8-yl)-3-oxopropylcarbamate (isopropyl) was dissolved in DCM (2 mL), HCl / i-PrOH (2 mL) was added dropwise, the reaction was stirred at room temperature for 3 h, and the reaction was concentrated to give the crude product, which was isolated and lyophilized to give 14 mg of a white solid. m / z: 499 (M+H). 1 H NMR(400MHz,CD3OD)δ8.32(d,J=25.7Hz,1H),7.68-7.32(m,4H),5.14(m,1H),4.71-4.28(m,3H),4.12-3.92(m,3H) ,3.80-3.40(m,3H),3.23-3.15(m,2H),2.24-2.16(m,1H),1.92-1.82(m,2H),1.80-1.70(m,1H),1.48-1.16(m,8H).

[0269] Example 37 Scheme N: [ka] Reaction conditions: a) triethylamine, di-t-butyl dicarbonate, dichloromethane, b) sodium bis(trimethylsilyl)amide (2.0 mol / L tetrahydrofuran solution), bromomethyl methyl ether, 2-methyltetrahydrofuran, c) (R)-4-benzyloxazolidin-2-one, diisopropylethylamine, trimethylacetyl chloride, toluene, d) titanium tetrachloride (1 mol / L toluene solution), diisopropylethylamine, dichloromethane, e) water peroxide. A) ethyl acetate solution (30%), lithium hydroxide monohydrate, tetrahydrofuran, water, f) t-butyl 2,5-diazabicyclo[4.1.0]heptane-2-carboxylate, 4-dimethylaminopyridine, N-methylpyrrolidone, g) hydrogen chloride in dioxane (4.0 M), h) 2-(7-benzotriazole oxide)-N,N,N',N'-tetramethyluronium hexafluorophosphate, diisopropylethylamine, N,N-dimethylformamide, i) hydrogen chloride in dioxane (4.0 M).

[0270] a) t-Butyl cyclopropylcarbamate Under a nitrogen gas atmosphere, cyclopropylamine (9.3 g) and triethylamine (19.7 g) were dissolved in dichloromethane (100 mL) at 20°C, di-t-butyl dicarbonate (35.48 g) was added dropwise at 0°C, and the reaction was carried out at 20°C for 16 hours. After the reaction was completed, the solvent was removed to obtain 24.3 g of a colorless liquid. 1 H NMR (400MHz, CDCl3) δ (ppm) 0.47-0.50 (m, 2H), 0.66-0.72 (m, 2H), 1.44 (s, 9H), 2.53 (m, 1H), 4.79 (s, 1H).

[0271] b) t-Butyl cyclopropyl(methoxymethyl)carbamate Under a nitrogen gas atmosphere, cyclopropylcarbamate t-butyl (24.3 g) was dissolved in 2-methyltetrahydrofuran (100 mL), sodium bis(trimethylsilyl)amide (120 mL) was added dropwise at 0°C, the reaction solution was stirred at 0°C for 1 hour, bromomethyl methyl ether (35.7 g) was added dropwise at 0°C, the reaction solution was stirred at 0°C for 6 hours, the reaction solution was poured into 50 g of ice water, the liquid was separated, and the reaction solution was extracted with ethyl acetate (100 mL * 2). The reaction solution was concentrated as it was to obtain 29.1 g of a colorless liquid as the product, which was used directly in the next step without purification.

[0272] c) (R)-4-benzyl-3-(2-(4-(chlorophenyl)acetyl)oxazolidin-2-one Under a nitrogen gas atmosphere, 2-(4-chlorophenyl)acetic acid (50g), (R)-4-benzyloxazolidin-2-one (45.5g), and diisopropylethylamine (127.3g) were dissolved in toluene (600mL), trimethylacetyl chloride (38.4g) was added dropwise at 15°C, the reaction solution was stirred at reflux for 16 hours, and the reaction solution was poured into 200mL of water, separated, washed with 120mL of saturated saline, and the organic phase was dried and concentrated to obtain a crude product. The crude product was isolated and purified by column chromatography (PE:EA=5:1) to obtain 32g of a white solid. 1 H NMR(400MHz,DMSO-d6)δ(ppm)2.88-3.02(m,2H),4.12-4.37(m,4H),4.64-4.70(m,1H),7.13-7.16(m,2H),7.23-7.32(m,5H),7.39-7.42(m,2H).

[0273] d) ((S)-3-((R)-4-benzyl-2-oxazolidin-3-yl)-2-(4-chlorophenyl)-3-oxopropyl)(cyclopropyl)carbamate t-butyl Under a nitrogen gas atmosphere, (R)-4-benzyl-3-(2-(4-(chlorophenyl)acetyl)oxazolidin-2-one (3.48 g) was dissolved in dichloromethane (60 mL), and a toluene solution of titanium tetrachloride (13 mL) was added dropwise at 0 ° C., the reaction solution was stirred at 0 ° C. for 2 hours, DIPEA (1.49 g) was added dropwise, the reaction solution was stirred at 0 ° C. for 1.5 hours, cyclopropyl (methoxymethyl) carbamate t-butyl (2.77 g) was added dropwise, and the reaction solution was stirred at 0 ° C. for 6 hours to complete the reaction. Then, the reaction solution was poured into 30 mL of saturated ammonium chloride solution, separated, washed with 120 mL of saturated saline, and the organic phase was dried and concentrated to obtain a crude product. The crude product was isolated and purified by silica gel column chromatography (PE:EA = 10:1) to obtain 2.50 g of a colorless oily product.

[0274] e) (S)-3-((t-butoxycarbonyl)(cyclopropyl)amino)-2-(4-chlorophenyl)propionic acid Dissolve lithium hydroxide monohydrate (0.63g) in water (18mL), add tetrahydrofuran (20mL), drop hydrogen peroxide solution (1.6mL) at 0℃, add ((S)-3-((R)-4-benzyl-2-oxazolidin-3-yl)-2-(4-chlorophenyl)-3-oxopropyl)(cyclopropyl)carbamate t-butyl (2.50g) at 0℃, stir the reaction solution at 0℃ for 3 hours, add saturated sodium sulfite solution (15mL) to the reaction solution, react for 1.5h, adjust pH=3~4 with saturated potassium hydrogen sulfate solution, extract with ethyl acetate (30mL*2), separate, dry and concentrate the organic phase to obtain a crude product. The crude product was isolated and purified by column chromatography (PE:EA=1:1) to obtain a colorless solid 1.26g. 1 H NMR(400MHz,DMSO-d6)δ(ppm):0.45-0.48(m,2H),0.60-0.64(m,2H),1.30(s,9H),2.19(s,1 H),3.61(d,J=7.6Hz,1H),3.95(t,J=8.0Hz,1H),7.37(dd,J=26.8,8.8Hz,4H),12.7(s,1H).

[0275] f) 5-((R)-5-methyl-7-oxo-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-4-yl)-2,5-diazabicyclo[4.1.0]heptane-2-carboxylic acid ester Under a nitrogen gas atmosphere, (R)-4-chloro-5-methyl-5,8-dihydropyridine[2,3-d]pyrimidin-7(6H)-one (300 mg), 2,5-diazabicyclo[4.1.0]heptane-2-carboxylate t-butyl (455 mg), and 4-dimethylaminopyridine (600 mg) were dissolved in N-methylpyrrolidone (5 mL) and stirred at 120 ° C for 12 h. The reaction solution was then poured into 50 mL of water, extracted with ethyl acetate (20 mL * 2), washed with 15 mL of saturated saline, the organic phase was dried to remove the solvent, and a crude product was obtained. The crude product was isolated and purified by silica gel column chromatography (PE:EA = 1:1 to 1:2) to obtain 400 mg of a yellow solid.

[0276] g) (5R)-4-(2,5-diazabicyclo[4.1.0]heptan-2-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one 5-((R)-5-methyl-7-oxo-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-4-yl)-2,5-diazabicyclo[4.1.0]heptane-2-carboxylic acid ester (400 mg) was dissolved in dioxane (5 mL), and hydrogen chloride dioxane solution (5 mL) was added dropwise and stirred at 25° C. for 2 hours to complete the reaction. The reaction solution was directly concentrated to obtain a yellow solid as a crude product, which was directly used in the next step.

[0277] h) t-Butyl ((S)-2-(4-chlorophenyl)-3-((1R,6S)-5((R)-5-methyl-7-oxo-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-4-yl)-2,5-diazabicyclo[4.1.0]heptan-2-yl)-3-oxopropyl)(cyclopropyl)carbamate Under a nitrogen gas atmosphere, (5R)-4-(2,5-diazabicyclo[4.1.0]heptan-2-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one (270 mg), compound 5 (389 mg), 2-(7-benzotriazole oxide)-N,N,N',N'-tetramethyluronium hexafluorophosphate (474 ​​mg), and diisopropylethylamine (671 mg) were dissolved in N,N-dimethylformamide (10 mL), and the reaction solution was stirred at 25°C for 3 hours to complete the reaction. Then, the reaction solution was poured into 50 mL of water, extracted with ethyl acetate (20 mL * 2), washed with saturated saline (10 mL * 3), and the organic phase was dried and concentrated to obtain a crude product. The crude product was isolated and purified by column chromatography (PE:EA=1:2) to give 320 mg of a yellow solid.

[0278] i) (R)-4-((1R,6S)-5-((S)-2-(4-chlorophenyl)-3-(cyclopropylamino)propionyl)-2,5-diazabicyclo[4.1.0]heptan-2-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one and (R)-4-((1S,6R)-5-((S)-2-(4-chlorophenyl)-3-(cyclopropylamino)propionyl)-2,5-diazabicyclo[4.1.0]heptan-2-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one t-Butyl ((S)-2-(4-chlorophenyl)-3-((1R,6S)-5((R)-5-methyl-7-oxo-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-4-yl)-2,5-diazabicyclo[4.1.0]heptan-2-yl)-3-oxopropyl)(cyclopropyl)carbamate (320 mg) was dissolved in dioxane (2.5 mL), hydrogen chloride dioxane (2.7 mL) was added dropwise, and the reaction was stirred at 25° C. for 14 hours to complete the reaction. The reaction mixture was then concentrated to obtain a crude product, which was adjusted to pH = 13-14 with saturated potassium carbonate solution, extracted with DCM (10 mL * 2), washed with water (10 mL), the solvent was removed, and the product was optically resolved by supercritical fluid chromatography to obtain Isomer 1 (61.2 mg) and Isomer 2 (31.2 mg).

[0279] Equipment and conditions for optical resolution: waters SFC200, column: Daicel Chiralcel AS, 250×30 mm ID, 5 μm, mobile phase: A is CO2, B is isopropanol (0.1% NH3H2O), A:B=70:30 (volume ratio), flow rate 60 mL / min, column temperature 38°C. Ultra-high performance convergence chromatography conditions: Column: Daicel Chiralcel AD, 2.1 × 150 mm ID, 3 μm, Mobile phase A: CO2, Mobile phase B: Isopropanol (0.1% DEA), Gradient: Time 0-8 min, B phase 5-40% (volume ratio), Flow rate: 1 mL / min, Column temperature 40 °C. Isomer 1: RT = 3.7 min, Isomer 2: RT = 4.6 min.

[0280] Isomer 1: LCMS (ESI) m / z: 481 (M+H). 1H NMR(400MHz,DMSO-d6)δ(ppm):0.03-0.12(m,2H),0.25-0.30(m,2H),0.66-0.70(m,1H),0.96-1.05(m,3H),1.35-1.40(m, 1H),1.93-2.11(m,2H),2.29-2.35(m,1H),2.67-2.77(m,2H),2.80-2.86(m,1H),3.03-3.25(m,4H),3 .39-3.48(m,1H),3.69-3.79(m,1H),4.24-4.34(m,2H),7.34-7.41(m,4H),8.17(s,1H),10.52(s,1H).

[0281] Isomer 2: LCMS (ESI) m / z: 481 (M+H). 1 H NMR(400MHz,DMSO-d6)δ(ppm):0.14-0.21(m,2H),0.30-0.37(m,2H),0.93-1.07(m,4H),2.03-2.34(m,3H),2.66-2.86(m,2H) ),3.10-3.25(m,4H),3.36-3.94(m,4H),4.07-4.15(m,1H),4.41-4.45(m,1H),7.32-7.42(m,4H),8.19(s,1H),10.48(s,1H).

[0282] Example 38 [ka] The target compound was prepared according to the method described in Scheme N, except for using t-butyl 3,8-diazabicyclo[3.2.0]octane-8-carboxylate instead of t-butyl 2,5-diazabicyclo[4.1.1]heptane-2-carboxylate. LCMS (ESI) m / z: 495 (M+H). 1HNMR(400MHz,DMSO-d6)δ(ppm):0.11-0.22(m,2H),0.29-0.37(m,2H),1.01(dd,J=30.8,6.8Hz, 3H),1.52-1.69(m,1H),1.72-1.93(m,3H),2.04-2.14(m,1H),2.25(d,J=16Hz,1H),2.32(d,J=13 .2Hz,1H),2.66-2.83(m,3H),3.11-3.26(m,3H),3.52(d,J=16Hz,1H),3.75-3.83(m,1H),4.09- 4.15(m,1H),4.45-4.63(m,2H),7.33-7.41(m,4H),8.14(d,J=32Hz,1H),10.57(d,J=9.6Hz,1H).

[0283] Example 39 Scheme P: [ka] Reaction conditions: a) t-butylamine, N,N-dimethylformamide, b) ethyl 2-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)cyclopropanecarboxylate, palladium acetate, tricyclohexylphosphine, potassium carbonate, toluene, water, c) sulfuric acid, dichloromethane, d) triethylamine, ethanol, e) t-butyl 3,8-diazabicyclo[3.2.1]octane-8-carboxylate, N,N-diisopropyl Propylethylamine, N,N-dimethylformamide, f) hydrogen chloride dioxane solution, g) (S)-3-((t-butoxycarbonyl)(isopropyl)amino)-2-(4-chlorophenyl)propionic acid, diisopropylethylamine, 2-(7-benzotriazole oxide)-N,N,N',N'-tetramethyluronium hexafluorophosphate, N,N-dimethylformamide, h) SFC, i) hydrogen chloride dioxane solution.

[0284] a) N-(t-butyl)-6-chloro-5-iodopyrimidin-4-amine 4,6-Dichloro-5-iodopyrimidine (4.00 g) was mixed with N,N-dimethylformamide (60 ml) by stirring, and t-butylamine (5.32 g) was added at room temperature under nitrogen gas atmosphere. The mixture was stirred at room temperature overnight. The mixture was then poured into water (300 mL) and extracted with ethyl acetate (2 x 50 mL). The organic phases were combined and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure to give a pale yellow solid (4.1 g).

[0285] b) 2-(4-(t-butyl)-6-chloropyrimidin-5-yl)cyclopropylformate ethyl N-(t-butyl)-6-chloro-5-iodopyrimidin-4-amine (4.10 g) and ethyl 2-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)cyclopropane-1-carboxylate (6.32 g) were dissolved in toluene (64.00 mL) and water (16.00 mL), tricyclohexylphosphine (1.475 g), palladium acetate (1.475 g) and potassium carbonate (0.59 g) were added, and the mixture was stirred overnight at 90° C. under nitrogen gas atmosphere. The mixture was then cooled to room temperature, diluted with water (100 mL) and extracted with ethyl acetate (2×100 mL). The organic phases were combined and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure, and the product was purified by chromatography on silica gel plates (petroleum ether:ethyl acetate=10:1) to obtain 2.50 g of a dark yellow solid as the product.

[0286] c) 2-(4-amino-6-chloropyrimidin-5-yl)cyclopropylformate ethyl Ethyl 2-(4-(t-butyl)-6-chloropyrimidin-5-yl)cyclopropylformate (2.50 g) was added to dichloromethane (40.00 mL). Sulfuric acid (4.94 g) was added dropwise at 0-5° C. under nitrogen gas atmosphere. The reaction solution was stirred at room temperature for 1 hour, then cooled to 0° C. and the mixture was neutralized with saturated aqueous sodium bicarbonate to pH=8. Extraction was performed with dichloromethane (2×50 ml). The organic phases were combined and dried over anhydrous magnesium sulfate. Filtration was performed and the filtrate was concentrated under reduced pressure to obtain 2.4 g of a yellow liquid.

[0287] d) 1-chloro-7,7a-dihydro-5H-cyclopropane[4,5]pyridine[2,3-d]pyrimidin-6(6aH)-one At room temperature, under nitrogen gas atmosphere, 2-(4-amino-6-chloropyrimidin-5-yl) ethyl cyclopropyl formate (2.40 g) and triethylamine (6.03 g) were added to 200.00 mL of ethanol solution. The reaction mixture was stirred at 80° C. overnight. Then the mixture was cooled to room temperature and concentrated in vacuum. The product was purified by chromatography on silica gel plate (petroleum ether:ethyl acetate=1:1) to obtain 1.05 g of pale yellow solid as product.

[0288] e) t-Butyl-3-(6-oxo-6,6a,7,7a-tetrahydro-5H-cyclopropanepyrido[4,5]pyrimidin-1-yl)-3,8-diazabicyclo[3.2.1]octane-8-carboxylate 1-Chloro-7,7a-dihydro-5H-cyclopropane[4,5]pyridine[2,3-d]pyrimidin-6(6aH)-one (0.20g) was dissolved in N,N-dimethylformamide (5mL) at 20℃, and 3,8-diazabicyclo[3.2.1]octane-8-carboxylate t-butyl (0.43g) and N,N-diisopropylethylamine (0.39g) were added. The reaction mixture was stirred at 110℃ for 24h. Then, the reaction solution was poured into water (100mL) and extracted with ethyl acetate (100mL). The organic phase was washed with saturated saline (100mL*3), dried over anhydrous sodium sulfate, and dried in an evaporator. The product was purified by chromatography on silica gel plate (petroleum ether:ethyl acetate=1:1) to obtain 0.30g of white solid as the product.

[0289] f) 1-3,8-diazabicyclo[3.2.1]octan-3-yl)-7,7-dihydro-5H-cyclopropane[4,5]pyrido[2,3-d]pyrimidin-6(6aH)-one 4M hydrogen chloride dioxane solution (2 mL) was added to t-butyl-3-(6-oxo-6,6a,7,7a-tetrahydro-5H-cyclopropanepyrido[4,5]pyrimidin-1-yl)-3,8-diazabicyclo[3.2.1]octane-8-carboxylate (0.15 g) at 20° C. The reaction mixture was stirred at 20° C. for 3 h. The reaction mixture was dried in an evaporator to give an off-white solid (0.12 g).

[0290] g) t-Butyl ((2R)-2-(4-chlorophenyl)-3-oxo-3-3-(6-oxo-6,6-a,7,7a-tetrahydro-5H-cyclopropyl-[4,5]pyridinepoly[2,3-d]pyrimidin-1-yl)-3,8-diazabicyclo[3.2.1]octan-8-yl)propylcarbamate (isopropyl) (S)-3-((t-butoxycarbonyl)(isopropyl)amino)-2-(4-chlorophenyl)propionic acid (0.18 g) and diisopropylethylamine (0.17 g) were added to 1-3,8-diazabicyclo[3.2.1]octan-3-yl)-7,7-dihydro-5H-cyclopropane[4,5]pyrido[2,3-d]pyrimidin-6(6aH)-one (0.12 g) in N,N-dimethylformamide (5 mL) at 20°C. The reaction mixture was stirred for 2 minutes. 2-(7-benzotriazole oxide)-N,N,N',N'-tetramethyluronium hexafluorophosphate (0.20 g) was added at 20°C, and the reaction mixture was stirred at 20°C for 1 h. The reaction mixture was poured into water (100 mL) and extracted with ethyl acetate (100 mL). The organic phase was washed with saturated saline (100mL*3), dried over anhydrous sodium sulfate, and dried in an evaporator. The product was purified by chromatography on a silica gel plate (petroleum ether: ethyl acetate = 1:1.5) to obtain 0.20g of a white solid as the product.

[0291] h) Optical splitting Equipment and conditions for optical resolution: waters SFC200, column: Daicel Chiralcel AS, 250×50 mm ID, 10 μm, mobile phase: A is CO2, B is ethanol (0.1% NH3H2O), A:B=60:40 (volume ratio), flow rate 60 mL / min, column temperature 38°C.

[0292] i) Preparation of Isomer 1 and Isomer 2 To the isomer 1a (20 mg) was added 4 M hydrogen chloride in dioxane (1 mL) at 20° C. The reaction mixture was stirred at 20° C. for 3 h. The reaction mixture was dried in an evaporator and isolated to give an off-white solid (10 mg).

[0293] In a similar manner, isomer 2 was prepared from isomer 2a. Isomer 1: LCMS (ESI) m / z: 495 (M+H) 1 H NMR(400MHz,DMSO-d6)δ8.69-8.59(m,1H),7.34-7.52(m,4H),5.39-5.53(m,1H),4 .69-4.81(m,3H),4.52-4.67(m,1H),4.09-4.38(m,2H),3.75-3.81(m,1H),3.64-3 .73(m,2H),3.45-3.53(m,2H),3.26-3.36(m,1H),2.95-3.06(m,1H),1.96-2.07(m ,1H),1.50-1.66(m,2H),1.21-1.30(m,8H),1.01-1.11(m,1H),0.83-0.90(m,1H).

[0294] Isomer 2: LCMS (ESI) m / z: 495 (M+H) 1H NMR(400MHz,DMSO-d6)δ10.43(d,J=15.9Hz,1H),8.20(d,J=26.6Hz,1H),7.37-7.51( m,4H),4.64-4.74(m,1H),4.46-4.61(m,2H),4.19-4.23(m,2H),4.09-4.14(m,2H),3 .67-3.73(m,2H),3.56-3.62(m,2H),2.97-3.13(m,2H),2.17-2.28(m,1H),1.96-2.1 0(m,2H),1.75-1.84(m,1H),1.62-1.68(m,1H),1.20-1.30(m,6H),0.67-0.77(m,1H).

[0295] Example 40 [ka] The mixture was prepared in the same manner as described in Scheme P of Example 39, except that t-butyl piperazine-1-carboxylate was used instead of t-butyl 3,8-diazabicyclo[3.2.1]octane-8-carboxylate, and separated by supercritical fluid chromatography. The Boc protecting groups were then deprotected to obtain isomers 1 and 2. Equipment and conditions for optical resolution: waters SFC200, column: Daicel Chiralcel AS, 250×50 mm ID, 10 μm, mobile phase: A is CO2 and B is isopropanol (0.1% NH3H2O), A:B=60:40 (volume ratio), flow rate 60 mL / min, column temperature 38°C.

[0296] Isomer 1: LCMS(ESI)m / z:469(M+H) 1H NMR(400MHz,DMSO-d6)δ10.49(s,1H),8.24(s,1H),7.34-7.52(m,4H),4.66-4.74(m, 1H),3.77-3.85(m,1H),3.60-3.73(m,4H),3.49-3.51(m,1H),3.43-3.48(m,2H),3.24 -3.35(m,2H),2.95-3.04(m,1H),2.80-2.92(m,1H),2.62-2.73(m,1H),2.21-2.29(m ,1H),2.01-2.11(m,1H),1.66-1.76(m,1H),1.21-1.27(m,6H),0.72(q,J=4.9Hz,1H).

[0297] Isomer 2: LCMS(ESI)m / z:469(M+H) 1 H NMR(400MHz,DMSO-d6)δ10.48(s,1H),8.22(s,1H),7.33-7.57(m,4H),4.59 -4.68(m,1H),3.64-3.72(m,4H),3.45-3.53(m,3H),3.27-3.36(m,2H),3.16 -3.24(m,1H),3.04-3.12(m,1H),2.93-3.03(m,1H),2.17-2.28(m,1H),1.9 9-2.09(m,1H),1.67-1.76(m,1H),1.19-1.31(m,6H),0.72(q,J=4.4Hz,1H).

[0298] Experimental Example 1 In vitro enzyme activity test 1. Materials and Reagents Envision plate reader (Molecular Devices) White 384-well plate (product number #264706, Thermo) Key Reagents Included in the HTRF kinEASE TK Reagent Kit (Product No. #62 TKOPEC, Cisbio) TK-biotin substrate Streptavidin-XL665 Europium-labeled tyrosine kinase substrate antibody 5x Enzyme Reaction Buffer SEB HTRF Testing Buffer AKT1 (Product number #01-101, Carna) AKT2 (Product number #01-102, Carna) AKT3 (Product No. PV3185, Invitrogen) ATP 10mM (Product No. PV3227, Invitrogen) DTT 1M (Product number #D5545, Sigma) MgCl21M (product number #M8266, Sigma) Compounds of the Invention Positive control: GDC-0068

[0299] 2. Experimental Procedure 2.1 Preparation of reagents Table 1. Components and concentrations in the kinase reaction system [Table 1] 1x Kinase Reaction Buffer 1 mL of 1x kinase reaction buffer for kinases AKT1, 2, and 3 contains 200 μL of 5x kinase reaction buffer, 5 μL of 1M MgCl2, 1 μL of 1M DTT, and 794 μL of ultrapure water.

[0300] 5x TK-Biotin Substrate and ATP Working Solution Specific concentrations of TK-biotin substrate and ATP are shown in Table 1.

[0301] Substrate and ATP were diluted to 5-fold the reaction concentration in 1x kinase reaction buffer.

[0302] 5x Kinase Working Solution The concentrations used for enzyme screening are shown in Table 1. A 5x enzyme working solution was prepared using 1x kinase reaction buffer.

[0303] 4x Streptavidin-XL665 working solution The concentration of streptavidin-XL665 in the reaction is shown in Table 1. A 4x streptavidin-XL665 working solution was prepared using assay buffer.

[0304] 4x Europium-labeled tyrosine kinase substrate antibody working solution As a working solution, a europium-labeled tyrosine kinase substrate antibody was diluted 100-fold with the assay buffer solution.

[0305] 2.2 Experimental scheme After preparing all the reagents by the above method, the reagents other than the enzyme were brought to room temperature and the addition of the sample was started.

[0306] a) First, compound stock solutions (10 mM DMSO solution) were diluted to 100 μM compound solutions with DMSO, and then diluted to 2.5 μM compound working solutions (containing 2.5% DMSO) with 1x kinase reaction buffer. 2.5% DMSO solutions were prepared with 1x kinase reaction buffer, and the 2.5 μM compound working solutions were diluted with 2.5% DMSO solution, and diluted 7 times with a 4-fold gradient to obtain a total of 8 concentrations (2500 nM, 625 nM, 156 nM, 39 nM, 9.8 nM, 2.4 nM, 0.6 nM, 0.15 nM) of compound working solutions. 4 μL of the diluted compound working solutions were added to all reaction wells except the control well, and 4 μL of the prepared 2.5% DMSO / kinase buffer was added to the control well.

[0307] b) 2 μL of the prepared TK-biotin substrate solution was added to all reaction wells (the concentrations of the substrates for enzyme screening are shown in Table 1).

[0308] c) Add 2 μL of the prepared enzyme solution to all reaction wells except the negative wells (enzyme concentration is shown in Table 1), and add 2 μL of the corresponding enzyme solution in 1x kinase reaction buffer to the negative wells to make up the volume. The plate was sealed with a sealing film, mixed uniformly, and then incubated at room temperature for 10 minutes to allow the compound and enzyme to bind sufficiently.

[0309] d) 2 μL of ATP solution was added to all reaction wells to initiate the kinase reaction (ATP concentrations and reaction times for enzyme screening are shown in Table 1).

[0310] e) 5 minutes before the kinase reaction was completed, start preparing the test solution. Using the test buffer in the reagent kit, prepare the test solution containing streptavidin-XL665 and europium-labeled tyrosine kinase substrate antibody (1:100) (the concentrations of the test reagents for enzyme screening are shown in Table 1).

[0311] f) After the kinase reaction was completed, 5 μL of diluted streptavidin-XL665 was added to all reaction wells, mixed uniformly, and then the diluted europium-labeled tyrosine kinase substrate antibody test solution was added immediately.

[0312] g) The plate was sealed and mixed evenly, and reacted at room temperature for 1 hour, after which the fluorescent signal was detected using an ENVISION (Perkinelmer) device (excitation at 320 nm, emission at 665 nm and 615 nm). The inhibition rate for each well was calculated from the wells with total activity and background signal, and the average value was calculated when multiple wells were tested. The median inhibitory activity (IC50) was fitted for each compound to be measured using the specialized plot analysis software PRISM 6.0. Table 2: Flow chart of sample addition for the experiment. [Table 2]

[0313] 2.3 Data analysis ER = 665 nm fluorescence value / 615 nm fluorescence value Inhibition rate=(ER 陽性対照 -ER サンプル ) / (ER 陽性対照 -ER 陰性対照 )*100% 3. Experimental Results The experimental results are shown in Table 3: Table 3: AKT inhibitory activity [Table 3-1] [Table 3-2] [Table 3-3] [Table 3-4] [Table 3-5] [Table 3-6] [Table 3-7] [Table 3-8] [Table 3-9] [Table 3-10] [Table 3-11] Experimental Example 2: Pharmacokinetic evaluation Formulation preparation of isomer 4 compounds of Example 10, Example 21, GDC-0068, and Example 34: Mixture solvent: Tween 80: PEG 400: water = 1: 9: 90 (v / v / v) Compounds were prepared as 10 mg / mL stock solutions in DMSO.

[0314] Accurately aspirate 400 μl of the 10 mg / mL stock solution into a glass bottle, add 3.6 mL of the mixed solvent, and the ratio of the solvents in the final formulation is DMSO:mixed solvent (v / v) = 10:90. Disperse the mixture evenly by vortexing (or sonication) to obtain a concentration of 1 mg mL for each compound. -1 Thus, 4 mL of a dosing solution was obtained.

[0315] Formulation preparation of isomer 2 compound of Example 15: Solvent: DMSO:PEG400:ultrapure water = 5:20:75 (v / v / v) In a glass bottle, 5.37 mg of the isomer 2 compound of Example 15 was weighed as a sample, 0.269 mL of DMSO was added, and the solid matter was completely dissolved. 1.074 mL of PEG400 was added, and the mixture was mixed uniformly by vortexing. 4.028 mL of ultrapure water was added, and the mixture was mixed uniformly by vortexing to a concentration of 1 mg mL. -1 A colourless solution of

[0316] Experimental animals: Mice, ICR strain, provided by Vital River Laboratory Animal Technology Co., Ltd., 6-10 weeks old, male. Experimental Design: Table 4: Animal experiment protocol [Table 4] The experimental animals were kept in the animal room of Shengsu New Drug Development Co., Ltd., Suzhou, China. The animal room was well ventilated and air-conditioned, with the temperature maintained at 20-25°C and the humidity maintained at 40%-70%. The light was bright and dark for 12 hours each, and the experimental animals had free access to food and water. After being normally kept for at least 5 days, mice in good physical condition were selected for this experiment after veterinary examination. Each mouse was labeled with its tail number. The details of the animal experiment plan are shown in Table 4.

[0317] After measuring the body weight, the theoretical administration volume for each mouse was calculated using the following formula:

number

[0318] The day before the experiment, mice were fasted overnight, had free access to water, and were fed 4 hours after dosing.

[0319] On the day of the experiment, mice in groups A to E were given 10 mg kg -1The administration solutions of Example 10, Example 21, GDC-0068, Isomer 2 of Example 15, and Isomer 4 of Example 34 were given by forced oral administration. After administration, about 100 μL of blood was collected from the mouse at each time point through the orbit and placed in an EDTA-K2 anticoagulant tube. The whole blood sample was centrifuged at 5,500 rpm for 10 min, and the separated plasma was stored in a refrigerator at -40 to -20 ° C. and used for the analysis of biological samples. An LC-MS / MS analysis method for measuring the concentration of the compound in mouse plasma was established and used to measure the concentration of the compound in the biological samples obtained in this experiment. Pharmacokinetic parameters were calculated using the non-compartment model of Pharsight Phoenix 7.0.

[0320] Experimental Results: The experimental results are shown in Table 5: Table 5: Pharmacokinetic parameters of compounds of the invention [Table 5] NR: Not calculated

Claims

1. Formula I: 【Chemistry 1】 or a pharma- ceutically acceptable salt thereof, Where: R １ is H, R ２ is an isopropyl group or a cyclopropyl group, R ３ is H, m is 1, n is 0, R ４ , R ５ together form =O, 【Chemistry 2】 teeth, 【Chemistry 3】 Selected from the group consisting of: R ６ , R ７ , R ８ , R ９ are each independently selected from the group consisting of H, CN, a C1-C6 alkyl group, and a C1-C6 alkoxy group, wherein said C1-C6 alkyl group or C1-C6 alkoxy group is optionally substituted with halogen, OH, CN, or a C1-C3 alkoxy group; R １０ is selected from the group consisting of H and a C1-C6 alkyl group, wherein said C1-C6 alkyl group is optionally substituted with a halogen, OH, CN, or a C1-C3 alkoxy group; L is the following group: 【Chemistry 4】 Selected from the group consisting of: G is any 1-5 R １１ is a 6-10 membered aryl or 5-10 membered heteroaryl group substituted with R １１ are independently halogen, OH, CN, NH ２ , NO ２ , benzyloxy group, -NH (C1-C6 alkyl group), -N (C1-C6 alkyl group) ２ , -C(=O)NH ２ , -C(=O)NH(C1-C6 alkyl group), -C(=O)N(C1-C6 alkyl group) ２ , -SO ２ (C1-C6 alkyl group), a C1-C6 alkyl group, and a C1-C6 alkoxy group, wherein the C1-C6 alkyl group or the C1-C6 alkoxy group is optionally substituted with a halogen, or a pharma- ceutically acceptable salt thereof.

2. The compound has formula IV: 【Chemistry 5】 2. The compound of claim 1 having the structure shown in: or a pharma- ceutically acceptable salt thereof.

3. The compound has formula V: 【Chemistry 6】 2. The compound of claim 1 having the structure shown in: or a pharma- ceutically acceptable salt thereof.

4. The compound has formula VI: 【Chemistry 7】 having the structure shown in 2. The compound of claim 1, or a pharma- ceutically acceptable salt thereof, wherein d is selected from the group consisting of 1 and 2.

5. The compound is 【Chemistry 8】 Having the structure shown in formula VII:

2. The compound of claim 1, or a pharma- ceutically acceptable salt thereof, wherein d is selected from the group consisting of 1 and 2.

6. The compound has formula VIII: 【Chemistry 9】 2. The compound of claim 1 having the structure shown in: or a pharma- ceutically acceptable salt thereof.

7. The compound is 【Chemistry 10】 Having the structure shown in formula IX:

2. The compound of claim 1, or a pharma- ceutically acceptable salt thereof, wherein d is selected from the group consisting of 1 and 2.

8. The compound has formula XI: 【Chemistry 11】 2. The compound of claim 1 having the structure shown in: or a pharma- ceutically acceptable salt thereof.

9. The compound is 【Chemistry 12】 10. The compound of claim 1 having the structure shown in Formula XII, where d is selected from the group consisting of 1 and 2, or a pharma- ceutically acceptable salt thereof.

10. 10. The compound of claim 4, 5, 7 or 9, or a pharma- ceutically acceptable salt thereof, wherein d is 1.

11.

13. teeth, 【Chemistry 14】 2. The compound of claim 1, selected from the group consisting of:

12.

15. teeth, 【Chemistry 16】 2. The compound of claim 1, selected from the group consisting of:

13.

17. teeth, 【Chemistry 18】 2. The compound of claim 1, selected from the group consisting of:

14.

19. teeth, 【Chemistry 20】 2. The compound of claim 1, wherein:

15. R ６ , R ７ , R ８ , R ９ are each independently selected from the group consisting of H, CN, a C1-C6 alkyl group, and a C1-C6 alkoxy group, wherein the C1-C6 alkyl group or the C1-C6 alkoxy group is optionally substituted with F, Cl, Br, I, OH, CN, or OMe, or a pharma- ceutically acceptable salt thereof.

16. R ６ , R ７ , R ８ , R ９ are each independently H, CN, a methyl group, an ethyl group, a propyl group, an isopropyl group, or CF ３ , C.H. ２ CF ３ , C.H. ２ CHF ２ , C.H. ２ CH ２ F, C.H. ２ C.N., C.H. ２ CH ２ C.N., C.H. ２ O.H., C.H. ２ CH ２ OH, OMe, OEt, OCH ２ CH ２ CH ３ 2. The compound of claim 1, or a pharma- ceutically acceptable salt thereof, wherein the compound is selected from the group consisting of:

17. R ６ H, CN, methyl group, ethyl group, propyl group, isopropyl group, CF ３ , C.H. ２ CF ３ , C.H. ２ CHF ２ , C.H. ２ CH ２ F, C.H. ２ C.N., C.H. ２ CH ２ C.N., C.H. ２ O.H., C.H. ２ CH ２ OH, OMe, OEt, OCH ２ CH ２ CH ３ or an isopropoxyl group, or a pharma- ceutically acceptable salt thereof.

18. R ６ H, CN, methyl group, ethyl group, isopropyl group, CF ３ , C.H. ２ CH ２ 2. The compound of claim 1, or a pharma- ceutically acceptable salt thereof, wherein R is 0, R is 1, R is 2, R is 3, R is 4, R is 5, R is 6, R is 7, R is 8, R is 9, R is 10, R is 11, R is 12, R is 13, R is 14, R is 15, R is 16, R is 17, R is 1

19. R ６ H, CN, methyl group, CF ３ or OMe, or a pharma- ceutically acceptable salt thereof.

20. R ６ is a methyl group or CF ３ 2. The compound of claim 1, wherein:

21. R ７ or a pharma- ceutically acceptable salt thereof.

22. R ８ H, CN, methyl group, ethyl group, propyl group, isopropyl group, CF ３ , C.H. ２ CF ３ , C.H. ２ CHF ２ , C.H. ２ CH ２ F, C.H. ２ C.N., C.H. ２ CH ２ C.N., C.H. ２ O.H., C.H. ２ CH ２ OH, OMe, OEt, OCH ２ CH ２ CH ３ or an isopropoxyl group, or a pharma- ceutically acceptable salt thereof.

23. R ８ H, CN, methyl group, ethyl group, isopropyl group, CF ３ , C.H. ２ CH ２ 2. The compound of claim 1, or a pharma- ceutically acceptable salt thereof, wherein R is 0, R is 1, R is 2, R is 3, R is 4, R is 5, R is 6, R is 7, R is 8, R is 9, R is 10, R is 11, R is 12, R is 13, R is 14, R is 15, R is 16, R is 17, R is 1

24. R ８ The compound according to claim 1, or a pharma- ceutically acceptable salt thereof, wherein is H, CN or a methyl group.

25. R ８ or a pharma- ceutically acceptable salt thereof.

26. R ９ or a pharma- ceutically acceptable salt thereof.

27. R １０ is selected from the group consisting of H and a C1-C6 alkyl group, wherein said C1-C6 alkyl group is optionally substituted with halogen, OH or CN, or a pharma- ceutically acceptable salt thereof.

28. R １０ is H, methyl group, ethyl group, propyl group, isopropyl group, CF ３ , C.H. ２ CF ３ , C.H. ２ CHF ２ , C.H. ２ CH ２ F, C.H. ２ C.N., C.H. ２ CH ２ C.N., C.H. ２ OH or CH ２ CH ２ 2. The compound of claim 1, or a pharma- ceutically acceptable salt thereof, wherein R is 0 or 1;

29. R １０ The compound according to claim 1, or a pharma- ceutically acceptable salt thereof, wherein is a methyl group.

30. L is the following group: 【Chemistry 21】 2. The compound of claim 1, selected from the group consisting of:

31. G is optionally 1-5 R １１ or optionally one or more R １１ 2. The compound according to claim 1, wherein R is a thienyl group or a pyridyl group substituted with R, or a pharma- ceutically acceptable salt thereof.

32. R １１ are independently F, Cl, Br, I, OH, CN, NH ２ , NO ２ , benzyloxy group, methyl group, ethyl group, isopropyl group, CH ２ CF ３ , C.F. ３ , SMe, OMe, OCF ３ 2. The compound according to claim 1, wherein the compound is selected from the group consisting of OEt and isopropoxy, or a pharma- ceutically acceptable salt thereof.

33. R １１ or a pharma- ceutically acceptable salt thereof, according to claim 1, wherein

34. The compound according to claim 1, or a pharma- ceutically acceptable salt thereof, wherein G is a phenyl group, a 2-chlorophenyl group, a 3-chlorophenyl group, a 4-chlorophenyl group, a 4-fluorophenyl group, a 4-bromophenyl group, a 4-methylphenyl group, a 4-ethylphenyl group, a 4-isopropylphenyl group, a 4-trifluoromethylphenyl group, a 4-cyanophenyl group, a 4-methoxyphenyl group, a 4-methylthiophenyl group, a 4-trifluoromethoxyphenyl group, a 4-chloro-3-fluorophenyl group, a 3,4-difluorophenyl group, a 2,4-dichlorophenyl group, or a 4-benzyloxyphenyl group.

35. 2. The compound according to claim 1, wherein G is a 4-chlorophenyl group, a 4-chloro-3-fluorophenyl group, a 4-trifluoromethylphenyl group, or a 3,4-difluorophenyl group, or a pharma- ceutically acceptable salt thereof.

36. The compound according to claim 1, or a pharma- ceutically acceptable salt thereof, wherein G is a 4-chlorophenyl group.

37. The compound or a pharma- ceutically acceptable salt thereof, 【Chemical 22】 【Chemistry 23】 【Chemistry 24】 【Chemistry 25】 cis-(5R)-4-(5-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)hexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one, or A compound which is trans-(5R)-4-(5-((S)-2-(4-chlorophenyl)-3-(isopropylamino)propionyl)hexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)-5-methyl-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one, or a pharma- ceutically acceptable salt thereof.

38. The compound or a pharma- ceutically acceptable salt thereof, 【Chemistry 26】 or a pharma- ceutically acceptable salt thereof.

39. 40. A pharmaceutical composition comprising a compound according to any one of claims 1-38, or a pharma- ceutically acceptable salt thereof.

40. Use of a compound according to any one of claims 1-38, or a pharma- ceutically acceptable salt thereof, or a pharmaceutical composition according to claim 39, in the preparation of a medicament for the prophylaxis and / or treatment of a disease or condition mediated by AKT protein kinase.

41. 40. Use of a compound according to any one of claims 1-38 or a pharma- ceutically acceptable salt thereof, or a pharmaceutical composition according to claim 39, in the preparation of a medicament for the prevention and / or treatment of breast cancer, prostate cancer, or ovarian cancer mediated by AKT protein kinase.

42. 13. A method for preparing a compound of claim 1 or a pharma- ceutically acceptable salt thereof, comprising: 1) preparing a compound of formula 1-2 from a compound of formula 1-1 in the presence of a base and a solvent; 2) preparing a compound of formula 1-3 from a compound of formula 1-2 and formamidine acetate in the presence of a base and a solvent; 3) preparing a compound of formula 1-4 from a compound of formula 1-3 in the presence of a base and a solvent; 4) further reacting the compound of formula 1-4 to produce a compound of formula 1-5; 5) reacting a compound of formula 1-5 with a compound of formula 11-1 to prepare a compound of formula 12-1; 6) the protecting group of the compound of formula 12-1 is deprotected to prepare a compound of formula 12-2; 7) reacting a compound of formula 12-2 with a compound of formula 1-9 to prepare a compound of formula 12-3; Including, 【Chemistry 27】 【Chemistry 28】 Where: R ２ is an isopropyl group or a cyclopropyl group, R ６ and R ８ are each independently selected from the group consisting of H, CN, a C1-C6 alkyl group, and a C1-C6 alkoxy group, wherein said C1-C6 alkyl group or C1-C6 alkoxy group is optionally substituted with halogen, OH, CN, or a C1-C3 alkoxy group; h is 1, j is 0, k is 1, G is any 1-5 R １１ is a 6-10 membered aryl or 5-10 membered heteroaryl group substituted with R １１ are independently halogen, OH, CN, NH ２ , NO ２ , benzyloxy group, -NH (C1-C6 alkyl group), -N (C1-C6 alkyl group) ２ , -C(=O)NH ２ , -C(=O)NH(C1-C6 alkyl group), -C(=O)N(C1-C6 alkyl group) ２ , -SO ２ (C1-C6 alkyl group), C1-C6 alkyl group, and C1-C6 alkoxy group, wherein the C1-C6 alkyl group or the C1-C6 alkoxy group is optionally substituted with halogen; P １ is H or an amino protecting group, and P ２ is an amino protecting group, P １ When is an amino protecting group, the method further comprises the step of removing the amino protecting group.

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