Outdoor stink bug repellent and method of use

Abstract

An object of the present invention is to provide a repellent substance for stink bugs, thereby preventing stink bugs from attaching to agricultural crops and preventing agricultural damage caused by stink bugs. [Solution] A stink bug repellent for outdoor use is provided, characterized in that the culture solution is an aqueous solution containing culture solutions of bacteria belonging to at least 14 genera, cultured so that the mass of the bacterial cells is 3 to 30 mass% of the mass of the culture solution when the total mass of the bacterial cells is taken as 100 mass%, and the aqueous solution contains as active ingredients fulvic acid, wood vinegar, and zinc ions.

Description

[Technical field]

[0001] The present invention relates to an outdoor stink bug repellent and a method for using the same, and more particularly to an outdoor stink bug repellent that is sprayed on fields and the like to prevent stink bugs from approaching cultivated crops and prevent damage to the crops, and a method for using the same. [Background technology]

[0002] It is known that there are many types of insects belonging to the Hemiptera order (hereinafter simply referred to as "stink bugs"), some of which have significantly different characteristics (see, for example, Non-Patent Document 1). However, stink bugs as a whole share the following characteristics:

[0003] Many stink bugs are nuisance pests that emit strong unpleasant odors, such as hexanal and trans-2-hexenal, when stimulated. These malodorous substances that produce unpleasant odors exhibit properties as stink bug warning signal substances, aggregation pheromones, sex pheromones, etc. (see, for example, Non-Patent Document 2). However, there are many types of stink bugs, different stink bugs emit different malodorous substances, and one type of stink bug may emit multiple malodorous substances, so there is a wide variety of malodorous substances, and the details, actions, and essence of these substances have not been elucidated.

[0004] Stink bugs have long, tubular mouthparts and suck the nutrient juices from a wide variety of agricultural products, such as beans, nightshade plants, potatoes, sweet potatoes, rice, tomatoes, and fruits, causing significant damage to those crops. Moreover, because stink bugs leave small bite marks, the damage can easily spread as farmers go unnoticed. Furthermore, if the unpleasant odor of stink bugs adheres to produce, the commercial value of the produce can decrease. Stink bugs are therefore pests that cause significant damage to agricultural products.

[0005] Stink bugs generally lay eggs in forests, grasslands, and fields around April and May and become adults in about a month, so they begin to multiply rapidly from around June. In some cases, a single male releases a sex pheromone that attracts a large number of females, causing them to multiply explosively. They then remain active until autumn, when they either semi-hibernate or migrate to warm places (such as the corners of houses) and become nuisance pests that give off a strong, unpleasant odor.

[0006] If stink bugs have infested agricultural produce, pesticides (insecticides) such as Benika (product name, Sumitomo Chemical Garden Products Co., Ltd.) are effective. However, using pesticides creates an economic burden and is not good for the environment, and there are some agricultural products for which pesticides cannot be used. Furthermore, if you try to forcibly exterminate stink bugs, they can produce a foul odor, which can reduce the value of the produce and make it unsellable. For this reason, research has been conducted to develop stink bug repellents (see, for example, Patent Documents 1 and 2), but a practical repellent has yet to be found. [Prior art documents] [Patent documents]

[0007] [Patent Document 1] JP 2023-77406 A [Patent Document 2] JP 2001-278722 A [Non-patent literature]

[0008] [Non-Patent Document 1] Toshikazu Yamashita et al., Study on the odorous components of stink bugs, Agricultural Research, 1979, 58, pp13-18 [Non-Patent Document 2] Tetsuya Yasuda, "Pheromones of stink bugs", Plant Protection, 2004, 58:7, pp14-18 [Non-Patent Document 3] "Fraction of fulvic acid by gel filtration and its chelating ability", Hidekazu Yamada, Koyo Yonebayashi, Kyosei Hattori, Shuji Morita, Abstracts of the 1972 Osaka Conference of the Japanese Society of Soil Science and Plant Nutrition (accepted July 26, 1975). https: / / core.ac.uk. / download / pdf / 235429797.pdf Summary of the Invention [Problem to be solved by the invention]

[0009] An object of the present invention is to provide an outdoor stink bug repellent that prevents damage to agricultural crops caused by stink bugs by spraying a substance that repels stink bugs on agricultural land to prevent stink bugs from attaching to the crops, and a method for using the same. [Means for solving the problem]

[0010] The outdoor stink bug repellent of the present invention, which solves the above problems, is a mixture of a culture liquid obtained by fermenting fermentable decomposable organic matter using a mixture of bacteria belonging to the following 13 fungal genera and a white-rot basidiomycete fungus, and water, culturing the mixture so that the total mass of the fungi accounts for 3 to 30% by mass of the total mass, decomposing the organic matter, and a mixture of fulvic acid, wood vinegar, zinc ions, and water, When the total mass of the mixed liquid is taken as 100% by mass, the mass of the culture liquid is 5 to 15% by mass, the mass of fulvic acid is 0.0001 to 0.01% by mass, the mass of wood vinegar is 0.2 to 10% by mass, and the mixed liquid contains 0.01 to 10.0 millimoles of zinc ions per 1 kg of the total weight of the mixed liquid. (1) Streptomyces (2) Sulfolobus (3) Saccharopolyspora (4) Nocardia (5) Pseudomonas (6) Bacillus (7)Cytophaga (8) Candida (9) Cellulomonas (10) Clostridium (11) Aspergillus (12) Penicillium (13) Rhizopus

[0011] A method for using the outdoor stink bug repellent of the present invention is characterized in that the outdoor stink bug repellent is diluted 20 to 50 times with water and sprayed. Effect of the Invention

[0012] By spraying the stink bug repellent of the present invention on agricultural crops during cultivation, it is possible to repel stink bugs from the crops and prevent damage to the agricultural crops caused by stink bugs. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0013] The outdoor stink bug repellent of the present invention is a mixed liquid obtained by mixing fulvic acid, wood vinegar, zinc ions, and water with a culture solution obtained by fermenting and cultivating fermentable decomposable organic matter.

[0014] The organic matter used as the raw material is not particularly limited as long as it is an organic matter that can be decomposed by fermentation. Preferred examples include, but are not limited to, waste from agricultural product processing, waste from marine product processing, and food waste.

[0015] [About the bacteria used in culture] The bacteria used in producing the stink bug repellent of the present invention consist of bacteria belonging to the following 13 genera and white-rot basidiomycetes, the genera being (1) Streptomyces, (2) Sulfolobus, (3) Saccharopolyspora, (4) Nocardia, (5) Pseudomonas, (6) Bacillus, (7) Cytophaga, (8) Candida, (9) Cellulomonas, (10) Clostridium, (11) Aspergillus, (12) Penicillium, and (13) Rhizopus.

[0016] The bacteria used to prepare the culture solution must contain all of the bacteria belonging to the 13 genera mentioned above and all of the white rot basidiomycetes. By containing all of the bacteria belonging to the 13 genera and all of the white rot basidiomycetes, the mutual decomposition and fermentation action of the bacteria can be increased.

[0017] [About the culture medium] The culture medium of the present invention is a mixture of bacteria belonging to the above-mentioned 13 kinds of fungal genera and a white-rot basidiomycete fungus, which is cultured by adding fermentable organic matter and water to the medium. These bacteria are preferably cultured so that the total mass of the bacterial bodies is 3 to 30% by mass relative to the total mass of the culture medium. If the mass of the bacterial bodies is less than 3% by mass relative to the mass of the culture medium, sufficient repellent effect against stink bugs cannot be obtained when used in a stink bug repellent, while it is often difficult to culture the bacteria so that the mass exceeds 30% by mass, and this is not preferred because the activity of the bacteria may decrease or change.

[0018] After the cultivation is completed, the culture liquid is filtered using a filter medium with openings large enough for the bacteria to pass through, and the culture residues, precipitates, and other debris are removed to obtain a culture liquid containing the bacteria. Here, water-soluble heavy metal components, including one or more of cadmium, mercury, and arsenic, contained in the organic matter, turn into water-insoluble solids and precipitate during cultivation, so when the solids are removed by filtration, the heavy metal components become water-insoluble and are removed.

[0019] [About fulvic acid] Fulvic acid is a substance that gives a yellow or orange-yellow color to the acidic supernatant when extracted with inorganic acid after humic acid has been extracted and removed from soil or coal with dilute alkali, and when dried, gives an amorphous powder. The powder obtained is an amorphous acidic substance that is soluble in water and ethanol. Fulvic acid does not have a single chemical structure, and its composition and molecular weight are not fixed. The composition and molecular weight change depending on the raw material and collection conditions. In practice, it is often used as an aqueous solution. In the present invention, it is preferable to use purified fulvic acid produced by the method described in Non-Patent Document 3.

[0020] [About wood vinegar] Wood vinegar is a pyrolysis liquid obtained as a distillate when wood is dry distilled, and it has been reported to have a weak repellent effect against stink bugs (see Patent Document 2). However, wood vinegar is expensive because only a small amount can be obtained even when a large amount of wood is dry distilled, and since its repellent effect against stink bugs is weak, it cannot be used for agricultural purposes, even if it can be used for home gardening. In contrast, in the present invention, the repellent effect against stink bugs can be significantly strengthened by adding a small amount of wood vinegar to a culture solution made of bacteria of 13 fungal genera and white-rot basidiomycetes.

[0021] [About zinc ions] When filamentous fungi are co-cultured in a cytoplasmic incompatible relationship, the fungal symbiotic virus does not spread (transfer) even if they are co-cultured, but when they are co-cultured in the presence of zinc ions, the virus migrates (transmits) from a virus infected with a fungal parasitic virus to a filamentous fungus that is not infected with the fungal infection virus, which is why zinc ions are used. In this way, the virus transfers through zinc ions, which increases the repellent effect on stink bugs.

[0022] [Composition of stink bug repellent] The stink bug repellent of the present invention is a mixed solution obtained by mixing the above culture solution, fulvic acid, wood vinegar, zinc ions, and water. In the stink bug repellent of the present invention, the mass of the culture solution is preferably 5% by mass to 15% by mass, assuming that the mass of the entire mixed solution is 100% by mass. If the mass of the culture solution is less than 5% by mass, the repellent effect of the stink bug repellent decreases, and even if the mass of the culture solution is added in an amount exceeding 15% by mass, the increase in the repellent effect of the stink bug repellent does not correspond to the increase in the amount of culture solution added, which is not preferable.

[0023] Furthermore, in the stink bug repellent of the present invention, when the mass of the entire mixed solution is taken as 100 mass%, the mass of fulvic acid is preferably 0.0001 to 0.01 mass%. If the mass of fulvic acid is less than 0.0001 mass%, the repellent effect of the stink bug repellent decreases, and adding fulvic acid in an amount exceeding 0.01 mass% is economically undesirable because the increase in the repellent effect of the stink bug repellent does not correspond to the increase in the amount of fulvic acid added.

[0024] Furthermore, the content of wood vinegar is preferably 0.2% by mass to 10.0% by mass when the mass of the entire mixture is taken as 100% by mass. If the mass of wood vinegar is less than 0.2% by mass, the repellent effect of the stink bug repellent decreases, and even if the mass of wood vinegar is added in an amount exceeding 10.0% by mass, the increase in the repellent effect of the stink bug repellent does not correspond to the increase in the amount of the added component, which is economically undesirable.

[0025] Furthermore, the zinc ion content is preferably 0.1 to 10.0 millimolar / stink bug repellent. If the zinc ion content is less than 0.1 millimolar, the repellent effect of the stink bug repellent decreases, and even if the zinc ion content exceeds 10 millimolar, the repellent effect of the stink bug repellent does not increase, which is economically undesirable.

[0026] [Example] (1)Culture solution The following 14 species of bacteria are included as the mixed fungus of the present invention, which is a mixture of bacteria of 13 genera and white-rot basidiomycetes. The attached symbols correspond to the above 13 genera. 1) Streptomycin-producing bacteria (Streptomyces griseus Leptomyces genus) 2) Sulfolobus acidoca ldarius Sulfolobus) 3) Saccharopolyspora erythraea erythraea (Saccharopolyspora genus) 4) Nocardia asteroides Cardia genus) 5) Pseudomonas fluorescens cens Pseudomonas genus) 6) Bacillus subtilis 7) Cytophaga hutchinsoni Cytophaga spp. 8) Candida versatilis ) 9) Cellulomonas flavigena Cellulomonas spp.) 10) Clostridium thermocellum llum Cristridium genus) 11) Aspergillus oryzae Gillus genus) 12) Penicillium chrysogenum m Penicillium spp. 13) Rhizopus oryzae (Rhizopus oryzae) In the present invention, a white-rot fungus is used as the white-rot basidiomycete.

[0027] Each of the 13 species of bacteria and white-rot basidiomycetes was mixed at 1 × 10 5 cfu / cm 3 The preculture solution was cultured until the total mass of the bacteria became 10% by mass, to produce a culture solution of the mixed bacteria. The culture solution was filtered using a filter medium with openings large enough for the bacteria to pass through, to obtain a culture solution.

[0028] (2) Wood vinegar 150 kg of logs (cedar, beech, cherry and oak) were charged into a wood distillation apparatus and heated to a temperature of 300°C or higher inside the vessel. The distillate was collected and allowed to stand, and the settled tar was removed to obtain 20 kg of wood vinegar as the supernatant.

[0029] (4) Stink bug repellents 200 kg of the stink bug repellent of the present invention was produced by adding water to 10 kg of the culture solution, 5.0 kg of fulvic acid produced according to the method described in Patent Document 3, 5.0 kg of wood vinegar, and 2.76 g (0.02 mol) of zinc chloride. In this case, the water used to produce the stink bug repellent is preferably water that does not contain an oxidizing agent.

[0030] In the present invention, the stink bug repellent produced as described above is diluted with water 20 to 50 times and then sprayed on farmland for use as a stink bug repellent. If the dilution rate is less than 20 times, the high concentration makes it difficult to handle. If the dilution rate exceeds 50 times, the repellent effect is not sufficient.

[0031] [Test example] (1) Test equipment Two plastic boxes, each 30 cm on a side, were arranged in a row with a cylindrical box 7 cm in diameter between them, and connected with a pipe 1 cm in diameter and 30 cm long to communicate with each other. A 9 cm diameter glass petri dish with 8.5 cm diameter filter paper laid on it was placed in the cylindrical box, and 0.5 mL of the sample was dropped onto the filter paper in one of the boxes to make it the sample side, and the same amount of water was dropped onto the other box to make it the control side. Ten narrow-spotted stink bugs were then placed in the central cylindrical box, and the number of stink bugs that moved after three days of rearing under conditions of 12 hours of light and 12 hours of darkness at room temperature while providing food and water was counted. The number of stink bugs that moved was divided into three types: the sample box, the control box, and other locations, and the number of stink bugs present in each was counted. The test was repeated 10 times. It was determined that the movement of stink bugs from the central stink bug introduction box to the test sample side was due to the test sample's attractive effect on the stink bugs, and that movement to the control side was due to the plant pyrolysis liquid's repellent effect on the stink bugs. The samples used for the measurements are shown below, and the test results are shown in Table 1. In addition, samples that did not contain zinc ions were excluded from the test because they did not show any repellent effect against stink bugs in preliminary tests.

[0032] (2) Samples used in the measurements Sample No. 1: Stink bug repellent described in the examples 10 parts by mass of culture solution, 0.005 parts by mass of fulvic acid, and 5 parts by mass of wood vinegar, 2.76 g of zinc chloride and water were added to make up to 100 parts by mass. Sample No. 2: 10 parts by mass of culture solution, 0.005 parts by mass of fulvic acid, and 2.76 g of zinc chloride and water to make up 100 parts by mass. This sample is an apple canker disinfectant. It is used as. Sample No. 3: 10 parts by weight of culture solution, 10 parts by weight of wood vinegar, 2.76 g of zinc chloride, and water. In addition, the amount was set to 100 parts by mass. Sample No. 4: 10 parts by weight of wood vinegar, 2.76 g of zinc chloride, and water were mixed to make 100 parts by weight. It was decided. Sample No. 5: 10 parts by mass of culture solution, 0.005 parts by mass of fulvic acid, and 2.76 g of zinc chloride and water were added to make up to 100 parts by mass. Sample No. 6 Water

[0033] (3) [Test results] [Table 1]

[0034] As shown in Table 1, the stink bug repellent of the present invention (sample number 1) exhibited a stronger repellent effect against stink bugs than the apple canker disinfectant (sample number 2) and wood vinegar (sample number 4). The mixture of culture solution and wood vinegar (sample number 3) also exhibited a strong repellent effect against stink bugs, but it was not as effective as the stink bug repellent of the present invention (sample number 1). The mixture of wood vinegar and fulvic acid (sample number 5) exhibited only the same repellent effect against stink bugs as fulvic acid (sample number 4).

Claims

1. A mixture of organic matter, a culture solution containing a mixture of bacteria belonging to the following 13 fungal genera and white-rot basidiomycetes, water, the total mass of the fungi being 3 to 30% by mass of the total mass of the culture solution, fulvic acid, wood vinegar, zinc ions, and water, When the total mass of the mixed solution is 100% by mass, the mass of the culture solution is 5% by mass to 15% by mass, the mass of the fulvic acid is 0.0001% by mass to 0.01% by mass, and the mass of the wood vinegar is 0.2% by mass to 10% by mass; An outdoor stink bug repellent comprising 0.01 to 10.0 millimoles of zinc ions per 1 kg of the total weight of the mixture. (1) Streptomyces (2) Sulfolobus (3) Saccharopolyspora (4) Nocardia (5) Pseudomonas (6) Bacillus (7) Cytophaga (Cytophaga) (8) Candida (9) Cellulomonas (10) Clostridium (11) Aspergillus (12) Penicillium (13) Rhizopus

2. 2. A method for using the outdoor stink bug repellent according to claim 1, comprising diluting the stink bug repellent with water 20 to 50 times and spraying the diluted stink bug repellent.

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