Adhesive material sampling tool, adhesive material inspection kit, and adhesive material sampling method

Abstract

To provide a deposit collection tool, although capable of keeping constant an area of a part for collecting deposits, of an object surface, allowing an inspector to collect the deposits being present on the object surface alone, deposit inspection kit and deposit collection method.SOLUTION: [1] A collection tool for collecting deposits being present on an object surface, includes a container body having an opening part c at one end and closed part f at the other end, and projections a and b provided at least at one of a side wall e and the closed part f of the container body, where the projections a and b are configured to rub the object surface when the container body rotates in a circumferential direction, with the opening part c side closely fitted to the object surface. [2] A deposit inspection kit includes the deposit collection tool described in [1] and a collection liquid stored inside the container body of the deposit collection tool. [3] A deposit collection method includes a step of rotating the container body in the circumferential direction, with the opening part c side of the deposit collection tool described in [1] closely fitted to the object surface.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to an adhering matter sampling tool, an adhering matter inspection kit, and an adhering matter sampling method. [Background technology]

[0002] When analyzing substances attached to the surface of an object in sanitary tests, environmental measurements, quality tests of industrial products, etc., the analysis results are greatly affected by the sampling tools and sampling methods used to collect the substances. Therefore, various sampling tools and sampling methods have been proposed to achieve the recovery rate of the substances and the reproducibility of the measurements according to the purpose. The same is true for the collection of attached bacteria for testing attached bacteria on human skin. A representative method for collecting adherent bacteria is the cup scrub method (see, for example, Non-Patent Document 1). This method is required by the American Society for Testing and Materials (ASTM), a standard method for evaluating antibacterial effects in the United States, when evaluating skin disinfection before surgery or before and after catheter insertion.

[0003] Specifically, in the cup scrub method, adherent bacteria are collected in the following manner. (1) A glass or metal cylinder is pressed against the surface of a target object, such as the skin. (2) Inject the bacterial collection liquid into the cylinder. (3) The surface of the object is scrubbed with a glass or metal rod for a certain period of time to disperse the bacteria into the bacterial collection solution. (4) A portion of the sample liquid in which the bacteria are dispersed is sucked up with a dropper and then dropped into the testing device. This collection method is excellent in terms of recovery rate of adherent bacteria and reproducibility of measurements for the following reasons. - Bacteria are dispersed in a bacterial collection solution, allowing a large amount of bacteria to be collected. By simply taking out a portion of the collection solution in which the bacteria are dispersed, the detection limit of the testing device is exceeded, making it possible to measure the amount of bacteria. By keeping the inner diameter of each cylinder constant, it is possible to always scrub a constant area of ​​skin. Therefore, when bacteria are collected on different days, it is possible to detect changes in the number of bacteria per unit area. In other words, quantitative measurement is possible.

[0004] Apart from the cup scrub method, a common method for collecting bacteria attached to the skin is the swab method, in which a sampling stick soaked in a sampling liquid is brought into contact with the skin and the skin is rubbed in that state to collect bacteria (see, for example, Patent Documents 1 and 2). [Prior art documents] [Patent documents]

[0005] [Patent Document 1] Japanese Patent Application Publication No. 10-229871 [Patent Document 2] JP 2011-69778 A [Non-patent literature]

[0006] [Non-Patent Document 1] Journal of Healthcare-associated Infection, 3(2), 19-23, 2010 Summary of the Invention [Problem to be solved by the invention]

[0007] Sampling bacteria on the skin and testing the condition of the bacteria is also important for keeping the skin healthy. By using medicines and cosmetics according to the condition of the bacteria, the condition of the skin can be kept optimal. Therefore, it is desirable for individuals to be able to sample bacteria on the skin frequently in their daily lives.

[0008] However, when an individual collects bacteria attached to the skin, the cup scrub method has the following problems when it is performed frequently on a daily basis. To collect attached bacteria, it is necessary to inject the bacterial collection liquid into the cylinder while pressing the cylinder against the skin. If the cylinder is not pressed against the skin, the collection liquid poured into one side of the cylinder will spill out the other side. This requires operation with both hands. Therefore, two people, an examiner and a test subject, are required to perform a skin attached bacteria test. For example, a person living alone cannot collect bacteria by himself in his own room. Furthermore, with the cup scrub method, only a portion of the collected fluid is used for measurement by the testing device. The cylinder cannot be removed from the skin until all unused collected fluid has been removed from inside the cylinder. Recovering the collected fluid requires the use of a dropper, which takes time, and during that time the cylinder must be pressed against the skin, placing a heavy burden on both the test subject and the tester. That is, the cup scrub method has the problem that it is difficult for one person to collect the deposits present on the surface of the object.

[0009] In addition, the swab method does not have a tool that determines the area to be sampled, such as the cylinder used in the cup scrub method. The skin is simply rubbed with a stick, and the area cannot be kept constant for each test. In addition, the sampled bacteria is squeezed out of the sampling stick and transferred to the testing device, but the amount of bacteria may vary depending on the squeezing technique. Therefore, although it can detect the presence or absence of bacteria, it is not suitable for quantitative measurement such as whether the number of bacteria has increased or decreased compared to the previous measurement. In other words, the swab method has the problem that the area on the surface of the target object from which the attached matter is collected cannot be kept constant.

[0010] The present invention has been made in consideration of the above circumstances, and provides an adhesion sampling tool, an adhesion inspection kit, and an adhesion sampling method that enable one person to sample adhesions present on the surface of an object while keeping the area of ​​the portion of the surface of the object from which adhesions are sampled constant. [Means for solving the problem]

[0011] According to the present invention, there are provided an adhesion sampling tool, an adhesion inspection kit, and an adhesion sampling method as described below.

[0012] [1] A sampling tool for sampling adhesions present on a surface of a target object, A container body having an opening at one end and a closing portion at the other end; a protrusion provided on at least one of the side wall portion of the container body and the closing portion; Equipped with The attachment sampling tool is configured so that the protrusions can rub the surface of the object when the container body is rotated in the circumferential direction while the opening side is brought into close contact with the surface of the object. [2] The adhesion sampling tool described in [1] above, in which the area of ​​the path described by the surface of the opening side of the protrusion when the container body is rotated once in the circumferential direction is S1, and the area of ​​the surface of the container body on the opening side is S2, satisfies the relationship S1 ≧ S2 × 0.5. [3] The protrusion portion has a first protrusion portion that protrudes toward the internal space of the container body and is supported by the side wall portion, and a second protrusion portion that protrudes toward the side wall portion and is supported by the closing portion. [4] The deposit sampling tool according to any one of the above [1] to [3], wherein the surface of the side wall portion facing the opening and the surface of the protrusion portion facing the opening are on approximately the same plane. [5] The deposit sampling tool according to any one of the above [1] to [4], wherein the container body is cylindrical. [6] The deposit sampling tool according to any one of the above [1] to [5], wherein the container body is made of resin. [7] The deposit sampling tool according to any one of the above [1] to [6], further comprising a lid member for closing the opening. [8] The deposit sampling tool according to [7] above, wherein the cover member is an easily peelable film. [9] The material collection tool according to any one of the above [1] to [8], wherein the object is skin.

[10] The deposit sampling tool according to any one of the above [1] to [9], wherein the deposit contains microorganisms.

[11] The deposit collection tool according to any one of [1] to

[10] above, A collection liquid contained inside the container body of the deposit collection tool; The adhesion inspection kit includes:

[12] A method for collecting adhesion material, comprising a step of rotating the container body in the circumferential direction while closely contacting the opening side of the adhesion material collecting tool according to any one of [1] to

[10] above against the surface of a target object. Effect of the Invention

[0013] According to the present invention, it is possible to provide an adhesion sampling tool, an adhesion inspection kit, and an adhesion sampling method that enable one person to sample adhesions present on the surface of an object while keeping the area of ​​the portion of the surface of the object from which adhesions are sampled constant. [Brief description of the drawings]

[0014] [Figure 1] 1 is a schematic diagram showing an example of an attachment sampling tool according to the present invention, in which FIG. 1(1) is a top view, FIG. 1(2) is a side perspective view, and FIG. 1(3) is a bottom perspective view. [Diagram 2] 1 is a schematic diagram for explaining the area S1 of the path traced by the surface on the opening side of the protrusion when the container body is rotated once in the circumferential direction, and the area S2 of the surface on the opening side of the container body. [Diagram 3] FIG. 2 is a top view showing an example of a deposit collecting tool according to the present invention. [Figure 4] FIG. 1 is a photograph showing an example of a deposit collection tool according to the present invention. [Diagram 5] FIG. 1 is a perspective view showing an example of an adhesion inspection kit according to the present invention. [Figure 6]FIG. 1 is a schematic diagram showing an example of a procedure for a method for collecting and inspecting a substance using a substance inspection kit according to the present invention. [Figure 7] FIG. 1 is a graph comparing the viable cell count of Staphylococcus epidermidis obtained by a method for inspecting adhesions using an adhesion sampling tool according to the present invention with the viable cell count of Staphylococcus epidermidis obtained by a cup scrub method. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0015] Hereinafter, an embodiment of the present invention will be described with reference to the drawings. In all the drawings, similar components are denoted by the same reference numerals, and the description thereof will be omitted as appropriate.

[0016] 1. Adhesive material collection tool Fig. 1 is a schematic diagram showing an example of an attachment sampling tool according to the present invention, in which Fig. 1(1) is a top view, Fig. 1(2) is a side perspective view, and Fig. 1(3) is a bottom perspective view. Fig. 4 is a photograph showing an example of an attachment sampling tool according to the present invention. The deposit sampling tool of the present invention is a sampling tool for sampling deposits present on the surface of an object, and comprises a container body having an opening c at one end and a closed portion f at the other end, and protrusions a, b provided on at least one of the side wall portion e and the closed portion f of the container body. In the deposit sampling tool of the present invention, the protrusions a, b are configured to be able to scrape the surface of the object when the container body is rotated in the circumferential direction while the opening c side is brought into close contact with the surface of the object. According to the deposit sampling tool of the present invention, the area of ​​the object surface from which deposits are sampled can be kept constant, and sampling of deposits present on the object surface can be performed by one person.

[0017] The deposit sampling tool according to the present invention has a structure that combines a container body for holding a sampling liquid and a protrusion that serves as a scrubbing means, so that the collection of deposits can be performed with one hand. Therefore, the collection of deposits present on the surface of an object can be performed by one person (substantially with one hand). In addition, in the deposit sampling tool according to the present invention, the area of ​​the portion from which deposits are sampled is determined by the inner diameter of the surface of the container body on the opening c side (hereinafter also referred to as the "upper surface"). This allows the deposit sampling tool according to the present invention to keep the area of ​​the portion from which deposits are sampled on the surface of the object constant, and allows deposits to be sampled from a substantially constant area. In this specification, the upper surface refers to the surface on the open side of the container body, which faces the closing portion f (hereinafter also referred to as the "bottom plate"). Furthermore, the deposit collection tool of the present invention can improve the recovery rate of deposits by rubbing the projections of the container body against the skin to lift off deposits on the surface and disperse the deposits in the collection liquid.

[0018] As mentioned above, the cylinder used in the cup scrub method must be pressed firmly against the skin with the hand holding it to prevent the collected liquid from spilling out from between the cylinder and the skin. At this time, the scrub stick is inserted from the open end of the cylinder, so the side wall of the cylinder is held. One end of the scrub stick must be held with the other hand, and the skin must be scrubbed evenly with the opposite end of the stick. For this reason, the cylinder must be of a certain size (thickness) for ease of use. However, as mentioned above, the cylinder needs to be resistant to deformation because a strong force is applied to the cylinder when the hand holding the side wall presses it against the skin. To achieve this, the side wall needs to be thickened, which increases the manufacturing cost for frequent use. In addition, the scrubbing wand is often fitted with a cap made of silicon rubber or the like at the tip to scrub efficiently. Therefore, the manufacturing cost of the scrubbing wand is also high for frequent use. On the other hand, in order to collect adhesions, the adhesion collection tool of the present invention only needs to be able to be pinched and rotated between the thumb and index finger. This allows for miniaturization. The side wall can also be reinforced with protrusions. This allows for a design using a resin with low strength. Therefore, the adhesion collection tool of the present invention can reduce the manufacturing cost of the collection tool, and can also reduce the price resistance to frequent collection of adhesions.

[0019] <Container body> The container body has an opening c at one end, a closing portion f at the other end, a side wall portion e between the opening c and the closing portion f, and an internal space surrounded by the closing portion f and the side wall portion e and capable of holding liquid. For example, one end of the container body is closed by a bottom plate to form a closed portion f, and the other end opposite the bottom plate is open to form an opening c. The shape of the container body is not particularly limited, and may be cylindrical, square tube, or the like. From the viewpoint of facilitating rotation of the container body, a cylindrical shape is preferred.

[0020] The material of the container body is not particularly limited, and examples thereof include resin materials, metal materials, inorganic materials, and wood. Among these, the material of the container body is preferably a resin material, because it has excellent heat sealability, moldability, oxidation resistance, sterilization resistance, recyclability, light weight, safety to the skin, etc., of the lid member described below. That is, the container body according to the present invention is preferably made of a resin. Examples of the resin material include polyethylene, polypropylene, polystyrene, polyethylene terephthalate (PET), and polyvinyl chloride.

[0021] <Protrusion> The protrusions a, b are supported by at least one of the side wall portion e and the closing portion f of the container body, and are configured to be able to rub the surface of the target object when the container body is rotated in the circumferential direction while the opening c side is in close contact with the surface of the target object. In addition, it is preferable that the protrusions a and b are configured so as not to divide the internal space of the container body so that the collected liquid is not divided in the internal space of the container body, thereby making it possible to prevent adhesions from being omnipresent in the internal space of the container body.

[0022] In the deposit sampling tool of the present invention, when the area of ​​the path described by the surface on the opening side of the protrusion when the container body is rotated once in the circumferential direction is taken as S1 and the area of ​​the surface on the opening c side of the container body is taken as S2, from the viewpoint of improving the amount of collected deposit and the recovery rate, it is preferable to satisfy the relationship S1 ≧ S2 × 0.5, it is more preferable to satisfy the relationship S1 ≧ S2 × 0.8, it is even more preferable to satisfy the relationship S1 ≧ S2 × 0.9, it is even more preferable to satisfy the relationship S1 ≧ S2 × 0.95, and it is even more preferable to satisfy the relationship S1 ≧ S2 × 0.99. Here, using Figure 2, we will explain the area S1 of the trajectory drawn by the surfaces of the protrusions a and b on the opening c side when the container body is rotated once in the circumferential direction, and the area S2 of the surface of the container body on the opening c side. First, area S2 is the area of ​​the surface of the container body on the opening c side, which is the area of ​​the circle on the top surface of the cylindrical container in the case of Fig. 2. In other words, area S2 is the sum of the area of ​​the opening c, the area of ​​the surfaces of protrusions a and b on the opening c side, and the area of ​​surface d of side wall e on the opening c side. The area S1 is the area of ​​the path drawn by the surfaces of the protrusions a and b on the opening c side when the container body is rotated once in the circumferential direction. In the case of FIG. 2, the area S of the path drawn by the surface of the protrusion a on the opening c side is 1a and the area S of the path drawn by the surface of the opening c of the protrusion b 1b is the total area of

[0023] The protrusion portion according to the present invention is not particularly limited as long as it has a shape capable of rubbing the surface of an object. For example, as shown in FIG. 1, it is preferable for the protrusion portion to have a first protrusion portion b that protrudes toward the internal space of the container body and is supported by the side wall portion e, and a second protrusion portion a that protrudes toward the side wall portion e and is supported by the closing portion f. In the example of Figure 1, the first protrusion b is supported by the side wall e and protrudes toward the internal space of the container body, and the second protrusion a is attached near the center of the bottom plate and protrudes toward the side wall e. In addition, the first protrusion b provided on the side wall portion e acts as a beam that prevents deformation of the side wall portion e, so that the thickness of the side wall portion e can be made thinner by providing the first protrusion b.

[0024] FIG. 3 is a top view showing an example of the deposit collecting tool according to the present invention. The protrusion of the present invention may have any shape that allows it to rub the surface of an object, and does not have to be cross-shaped as shown in FIG. 1. For example, it may have any of the shapes shown in (1) to (4) of FIG. 3.

[0025] In the deposit sampling tool according to the present invention, as shown in FIG. 1, for example, it is preferable that the surface d of the side wall e on the opening c side and the surfaces of the protrusions a, b on the opening c side are substantially on the same plane. That is, it is preferable that the surface (top surface) of the side wall portion e on the open end side and the surfaces (top surfaces) of the protrusions a and b on the open end side are substantially on the same plane. In this specification, "substantially on the same plane" refers to surfaces whose perpendicular distance between each other is, for example, 0.5 mm or less, preferably 0.3 mm or less, more preferably 0.1 mm or less.

[0026] The material of the protrusion is not particularly limited, and examples thereof include resin materials, metal materials, inorganic materials, and wood. Among these, resin materials are preferred as the material for the protrusions because they are excellent in moldability, oxidation resistance, sterilization resistance, recyclability, light weight, safety to the skin, etc. That is, the protrusions according to the present invention are preferably made of resin. Examples of the resin material include polyethylene, polypropylene, polystyrene, polyethylene terephthalate (PET), and polyvinyl chloride. Furthermore, from the viewpoint of improving the moldability of the deposit sampling tool according to the present invention, the protrusion is preferably integrated with the container body, and more preferably is integrally molded with the container body using a resin material.

[0027] <Cover parts> The deposit sampling tool according to the present invention may further include a lid member g for closing the opening c. The lid member g is for closing the opening c of the container body and sealing the internal space of the container body. By pouring a sampling liquid into the internal space of the container body and sealing the opening c with the lid member, an deposit inspection kit described below can be obtained. The cover member g is not particularly limited, but for example, a resin film or a metal foil can be used, and it is preferable that the cover member g is an easily peelable film because it can be easily opened when used. As the easily peelable film, for example, films using a peeling mechanism such as interfacial peeling, cohesive peeling, and interlayer peeling are known, and a suitable film can be selected from known films according to the material of the container body and the desired performance. In general, a laminate film having a polymer blend fusion layer on a base film, a laminate film having a hot melt fusion layer on a base film, an interface peeling laminate film having a seal layer or a peeling layer, etc. can be used.

[0028] <Object> The subject from which the deposit collection tool of the present invention is used to collect deposits is not particularly limited, but examples include skin, mucous membrane, feces, food, clothing, daily necessities, soil, medical instruments, industrial products, etc. Among these, the deposit collection tool of the present invention is preferably used to collect deposits present on the skin surface. That is, the subject from which the deposit collection tool of the present invention is used to collect deposits is preferably skin.

[0029] <Attachment> The deposits to be collected by the deposit collection device of the present invention are not particularly limited, but examples include microorganisms such as bacteria, fungi, viruses, microalgae, protozoa, and mold; biological substances such as proteins, amino acids, lipids, nucleic acids, carbohydrates, vitamins, hormones, peptides, minerals, sebum, triglycerides, diglycerides, fatty acids, and glycerin; metals, polymers, various fine particles, and various foreign matter. Among these, the deposit collection tool of the present invention can be suitably used to collect microorganisms (preferably bacteria). That is, the deposits to be collected by the deposit collection tool of the present invention are preferably microorganisms, more preferably bacteria, and more preferably bacteria present on the skin surface. Bacteria present on the skin surface include, for example, skin resident bacteria. Skin resident bacteria refers to bacteria growing on human skin, but in the present invention, the species of bacteria is not particularly limited. Examples of skin resident bacteria include Staphylococcus epidermidis, Staphylococcus aureus, Propionibacterium acnes, Corynebacterium, etc.

[0030] 2. Adhesion inspection kit FIG. 5 is a perspective view showing an example of an adhesion inspection kit according to the present invention. The adhesion inspection kit according to the present invention comprises the adhesion sampling tool according to the present invention described above, and sampling liquid h contained inside the container body of the adhesion sampling tool. In the foreign matter inspection kit according to the present invention, it is preferable that the foreign matter sampling tool further comprises a cover member g for closing the opening c. With this, the collected liquid h can be contained within the container body by storing the collected liquid h in the internal space of the container body and sealing the opening c with the lid member g, which can improve the storage stability, handling, portability, etc. of the adhesion inspection kit before use. The preferred embodiment of the cover member g is the same as the cover member described above. The collection liquid h can be appropriately selected from known ones depending on the object and the attached matter. When the attached matter is bacteria, for example, physiological saline can be used as the collection liquid h.

[0031] 3. How to collect the attached material The method for collecting adhesions using the adhesion sampling tool of the present invention includes, for example, a step of rotating the container body in the circumferential direction while closely contacting the opening c side of the adhesion sampling tool of the present invention with the surface of the object. For example, a sampling liquid is poured into the container body, and the container body is rotated in the circumferential direction while pressing the surface of the object with the opening c side of the container body, that is, the container body is rotated around the normal line of the upper surface. This causes the protrusions to scrub the surface of the object, dispersing the adhesions present on the surface of the object into the sampling liquid, and the adhesions can be collected.

[0032] Hereinafter, with reference to Figs. 5 and 6, an example of the procedure for the method of sampling and testing bacteria present on the skin surface using the substance test kit according to the present invention will be described in more detail. As shown in FIG. 5, the internal space of the container body of the adhesion inspection kit is filled with a sampling liquid h, and the upper surface is sealed with a lid member g such as an easily peelable film so that it can be easily opened. First, the cover member g is peeled off from the container body to open the adhesion inspection kit (FIG. 6(1)). Next, while being careful not to spill the collection liquid contained inside the container body, the surface of the container body on the opening side c is pressed against the area from which bacteria are to be collected so that it is in close contact (Figure 6 (2)). Next, as shown in Figure 6 (3), the container is turned upside down so that the collection liquid touches the skin. In this state, the container body is rotated in the circumferential direction while pressing the surface of the opening c of the container body against the skin surface. This operation causes the protrusions of the attachment collection tool to move through the collection liquid while touching the skin surface, and to stir up the bacteria adhering to the skin surface into the collection liquid. The movement of the protrusions in the collection liquid further stirs the liquid, dispersing the bacteria that have been stirred up from the skin surface into the collection liquid. Next, turn it upside down again to return it to the state shown in Figure 6 (2), and remove the container body from the skin, taking care not to spill the collected liquid inside. Next, the collected liquid in which the bacteria are dispersed within the container body is sucked up using a dropper or the like (Figure 6 (4)). Next, the collected liquid is transferred to a testing device (testing tool) and measured (Figure 6 (5)). The method for testing the collected liquid containing the attached matter can be appropriately selected from among known testing methods depending on the object and the attached matter. In addition, the above describes an example of using an adhesion inspection kit in which the adhesion sampling device according to the present invention is filled with sampling liquid and sealed with a lid member, but a method in which the subject himself / herself pours the sampling liquid from a separately stored container into the sampling device and uses the sampling device may also be used. EXAMPLES

[0033] The present invention will be specifically described below with reference to examples, but the present invention is not limited to these examples in any way.

[0034] Example 1 Using a 3D printer, the collection tool shown in Figure 4 (bottom area 1 cm 2 After sterilizing the obtained sampling tool, 1 mL of the sterilized bacterial sampling solution was placed in the sampling tool. As the sampling solution, physiological saline containing polyoxyethylene (20) sorbitan monolaurate (manufactured by Fujifilm Wako Pure Chemical Industries, Ltd.) as a surfactant was used. Here, the skin surface is covered with sebum, which cannot be washed off effectively with water, so a surfactant is added to the collection solution in order to dissolve the sebum in the collection solution. Note that it was confirmed in advance that the surfactant concentration used in the experiment does not lyse Staphylococcus epidermidis. In addition, physiological saline is used to prevent bacteria from dying due to the sudden change in osmotic pressure when they move from the skin surface, which has a high salt concentration, to the collection solution, which has a low salt concentration.

[0035] Next, the sampling tool was pressed against the cheek while being careful not to spill the sampling liquid, the angle was adjusted so that the skin surface was evenly wetted with the sampling liquid, and the tool was rotated several times using the thumb and index finger around the axis normal to the contact surface. After removing the sampling tool from the skin surface while being careful not to spill the sampling liquid, the sampling liquid was collected using a micropipette, and the number of S. epidermidis colonies was measured using the plate dilution method. The resulting bacterial count was 3.63±0.77 LogCFU / cm 2This number was 2.77±0.98 LogCFU / cm2 obtained from the cheeks of 80 healthy women using the cup scrub method. 2 ) (J. Soc. Cosmet. Chem. Jpn. 28 (1) 44-56 (1994)) (see Figure 7). From the above, it was found that the use of the sampling tool of the present invention can provide a high bacterial sampling rate that is comparable to that of the cup scrub method. [Explanation of symbols]

[0036] a Projection (second projection) b Projection (first projection) c opening d. Surface of the side wall facing the opening e Side wall f Closed part g Cover member h Collection liquid

Claims

1. A sampling tool for sampling adhesions present on a surface of a target object, A container body having an opening at one end and a closing portion at the other end; a protrusion provided on at least one of the side wall portion of the container body and the closing portion; Equipped with The protrusion protrudes toward an internal space of the container body, a surface of the side wall portion facing the opening and a surface of the protrusion portion facing the opening are substantially flush with each other, The attachment sampling tool is configured so that the protrusions can rub the surface of the object when the container body is rotated in the circumferential direction while the opening side is brought into close contact with the surface of the object.

2. The area of ​​the path drawn by the surface of the protrusion on the opening side when the container body is rotated once in the circumferential direction is defined as S １ and the area of ​​the surface of the container body on the opening side is S ２ Then, S １ ≧S ２ 2. The material collecting tool according to claim 1, which satisfies the relationship of x=0.

5.

3. 3. The matter collecting tool according to claim 1 or 2, wherein the protrusion comprises a first protrusion protruding toward the internal space of the container body and supported by the side wall portion, and a second protrusion protruding toward the side wall portion and supported by the closing portion.

4. 3. The material sampling tool according to claim 1, wherein the container body is cylindrical.

5. 3. The material sampling tool according to claim 1, wherein the container body is made of resin.

6. 3. The deposit sampling tool according to claim 1, further comprising a lid member for closing said opening.

7. 7. The material collecting tool according to claim 6, wherein the cover member is an easily peelable film.

8. 3. The material collection tool according to claim 1, wherein the object is skin.

9. The deposit sampling tool according to claim 1 or 2, wherein the deposit includes microorganisms.

10. The deposit collector according to claim 1 or 2, A collection liquid contained inside the container body of the deposit collection tool; An adhesion inspection kit comprising:

11. 3. A method for collecting adhering matter, comprising the step of rotating the container body in a circumferential direction while bringing the opening side of the adhering matter collecting tool according to claim 1 or 2 into intimate contact with a surface of a target object.

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