Anti-ANGPT2 Antibodies

Abstract

The present invention relates to novel anti-Angiopoietin 2 (ANGPT2) neutralizing antibodies and compositions using same for therapeutic and diagnostic methods.

Description

[Technical field]

[0001] Sequence Listing This application contains a Sequence Listing that has been submitted electronically in ASCII format and is incorporated herein by reference in its entirety. Said ASCII copy, created on June 11, 2019, is named 09-0697-US-1_SL.txt and is 78,465 bytes in size. The present invention generally relates to anti-angiopoietin 2 (ANGPT2) neutralizing antibodies for diagnostic and therapeutic use. More specifically, anti-ANGPT2 antibodies and methods of use are disclosed for treating various diseases or disorders characterized by cells expressing ANGPT2. Pharmaceutical compositions and kits comprising anti-ANGPT2 antibodies are also disclosed. [Background technology]

[0002] Endothelial dysfunction is a hallmark of chronic kidney disease (CKD) and associated cardiovascular complications. Basic and clinical studies suggest that improving renal vascular function in CKD reduces proteinuria and slows the decline in renal function in addition to SOC. In addition, reduction of ANGPT2 is expected to have a positive effect on cardiovascular disease in CKD patients, including heart failure, MI, and stroke (Eleuteri 2011, Lukasz 2013, Poss 2015, Lorbeer 2015, Tsai 2016, Gerstein 2015, Chen 2009, Chen 2010a, Chen 2010b, Gurnik 2016). The human ANGPT-Tie axis consists of two type I tyrosine kinase receptors (Tie1, Tie2) and two secreted ligands (ANGPT1, ANGPT2). ANGPT1 is a Tie2 receptor agonist that induces receptor phosphorylation and activates downstream signaling pathways required for preserving renal vascular function, whereas ANGPT2 is a functional antagonist of ANGPT1 that binds to Tie2. ANGPT1-bound Tie2 translocates to interendothelial cell junctions where multimeric ANGPT1 may form cross-endothelial complexes with Tie2 receptors from adjacent cells to stabilize renal glomerular capillary structures. Intracellularly, ANGPT1-induced Tie2 phosphorylation leads to the recruitment of adaptor proteins, resulting in activation of the AKT pro-survival pathway and suppressing activation of the apoptotic pathway.

[0003] A small study investigating the role of ANGPT2 in diabetic nephropathy (DN) described a SNP (1233A / G) associated with a 20% increase in circulating ANGPT2 and subsequent increased DN severity (Quan, 2012). Although ANGPT2 blocking antibodies were not tested in CKD patients, ANGPT2 mRNA expression in Pima Indians with CKD was found to positively correlate with interstitial and intimal fibrosis. Others have shown that renal glomerular ANGPT2 mRNA expression is elevated in diabetic nephropathy (DN) patients compared to levels from non-diseased kidneys (Dessapt-Baradez, 2014). However, ANGPT1 renal glomerular mRNA expression (Dessapt-Baradez, 2014) and circulating protein (Chang, 2013; Chang, 2014) are both unchanged in DN, thus favoring ANGPT2-binding Tie2 in the context of disease progression. Important clinical observations related to dysregulation of the ANGPT2-Tie2 pathway with CKD come from studies where patients were stratified by stage (stage 1 to ESRD / HD) and circulating ANGPT2 was progressively elevated, correlated with arterial stiffness (Chang, 2014), and inversely correlated with declines in inulin-measured glomerular filtration rate (GFR) (David, 2010). In a cohort of CKD3-5 patients, ANGPT2 levels were also associated with severity of albuminuria and markers of systemic microinflammation (Chang, 2013). At least part of the elevated ANGPT2 present in CKD may be due to a reduction in miR-145, which normally represses ANGPT2 transcription and has been demonstrated to be significantly reduced in stage 3-5 CKD patients (Chen, 2013). A recent poster presented at the American Society of Nephrology (Peters, 2018) showed that in patients with non-proliferative diabetic retinopathy with baseline albuminuria >30 mg / g, stimulation of the vascular endothelial (VE) protein tyrosine phosphatase, Tie2 signaling using AKB-9778 (daily sc injections), was sufficient to reduce urinary albumin-to-creatine ratio (UACR) by approximately 20% in a 3-month Phase 2A study. These results support that stimulation of Tie2 signaling in patients with severe albuminuria is sufficient to reduce CKD progression. Clinically important values ​​for classifying CKD patients are: 15-60 ml / min / 1.73 m 2 and an UACR of 30 mg / g or greater.

[0004] Consistent with the notion that dysregulation of the ANGPT2-Tie2 axis contributes to CKD, preclinical studies have demonstrated that genetic manipulation of either side of the pathway (reducing ANGPT1 or increasing ANGPT2) is sufficient to cause disease manifestation. Conditional deletion of ANGPT1 in mice induces proteinuric nephropathy characterized by glomerular filtration barrier dysfunction, albuminuria, and pathological features seen in humans with advanced diabetic nephropathy (increased mesangial matrix and glomerulosclerosis; Jeansson, 2013). In addition, podocyte-specific ANGPT2 overexpression results in increased albuminuria, glomerular endothelial apoptosis, and reduced filtration barrier proteins (Davis, 2007). Elsewhere, plasma and renal expression of ANGPT2 was shown to be elevated after 5 / 6 nephrectomy in CD1 mice, concomitant with increased ANGPT2 staining in glomeruli (Chang, 2014). The same group, although they did not examine the effect on renal fibrosis, demonstrated that the ANGPT2 peptibody, L1-10, blocked vascular expression and upregulation of multiple profibrotic and proinflammatory markers, including TGFβ1, collagen subtypes and adhesion molecules, after 5 / 6 nephrectomy.

[0005] ANGPT2 is primarily expressed in tissues undergoing vascular remodeling and is elevated in the circulation in multiple disease states, including CKD. Endothelial cells (ECs) produce and store ANGPT2 in specialized storage granules, Weibel-Palade corpuscles, that can rapidly release ANGPT2 into the circulation upon binding to blood and lymphatic EC Tie2 receptors (Fiedler, 2004). In normal human kidneys, ANGPT2 and Tie2 are expressed in ECs within capillary loops, including those facing the glomerular basement membrane, and in glomerular ECs. Tie2 is also expressed in ECs adjacent to podocyte foot processes (Satchell, 2002). The Tie2 agonist ligand, ANGPT1, is secreted from pericytes, which surround and support the underlying endothelial cells ( Satchell, 2001 ), and also from specialized kidney cells, podocytes, which importantly comprise the glomerular filtration barrier, thereby allowing crosstalk between podocytes and adjacent glomerular ECs to stabilize the glomerular capillary structure ( Satchell, 2002 ).

[0006] ANGPT2 has limited expression in normal tissues but is widely expressed in the actively remodeling vasculature of human tumors. Blocking ANGPT2 inhibition of Tie2 signaling is an attractive target for antiangiogenic cancer therapy and vascular-based eye diseases. Several antibodies that block ANGPT2 binding to Tie2 have been developed for clinical use. Specifically, based on studies with an IV-administered ANGPT2-selective antibody (REGN910), no dose-limiting safety concerns were noted in phase I clinical trials (Papadopoulos, 2016). A Tie2-stimulator (AKB-9778) was tested in phase II with no notable adverse effects (AEs) (Campochiaro, 2016), and a dual ANGPT1 / 2 blocker (AMG386) was tested in multiple clinical studies up to phase III with only mild and reversible AEs (Monk, 2014). However, less selective treatment approaches using low ratio ANGPT2:ANGPT1 blockade (e.g. AMG386, MEDI3617) were accompanied by increased observations of clinical edema that may be associated with dual blockade of lymphatic Tie2 receptors, as both ANGPT1 and ANGPT2 function as Tie2 receptor agonists in the lymphatic vasculature. It is believed that blockade of Tie2 disrupts the normal flow of lymphatic and venous circulation, resulting in extracellular fluid accumulation in general and lymphedema specifically (Monk, 2013). Highly specific ANGPT2 blocking antibodies are expected to significantly reduce the risk of edema; grade 3-4 edema was not observed in a phase I study with REGN910 (Papadopoulos, 2015).

[0007] ANGPT2 blockade may affect vascular and lymphatic responses and function. ANGPT2 reportedly plays a role in liver regeneration (Hu, 2014); reducing vascular bed plasticity; altering healing of liver and other tissues (Gale, 2002), potentially including lung, adipose (An, 2017) and ovarian (Coxon, 2010) tissues; and acting as an autocrine regulatory switch for endothelial cell inflammatory responses (Fiedler, 2006; Kim, 2016). The role of ANGPT2 in the adult lymphoid system is not fully understood, however, Tie2 is expressed in multiple leukocyte types (monocytes, neutrophils, and eosinophils) and modulation of Tie2 signaling may alter immune sensitivity (Grenga, 2015).

[0008] ANGPT2 blockade is an attractive means to prevent other respiratory disorders, including pulmonary vascular hyperpermeability, pulmonary edema, acute respiratory distress syndrome (ARDS), acute lung injury (ALI), idiopathic interstitial pneumonia, idiopathic pulmonary fibrosis (IPF) and acute exacerbation of IPF, severe acute respiratory syndrome (SARS), and Middle Eastern respiratory syndrome (MERS). High plasma levels of ANGPT2 play a central role in abnormal vascular leakage associated with plasma increase of Willebrand factor (a standard marker of endothelial injury) in sepsis and ARDS (Calfee, 2012). ANGPT2 and Willebrand factor plasma levels are significantly elevated in patients with sepsis and even higher in patients with ARDS (Van der Heijden, 2008). Circulating ANGPT2 was significantly elevated in humans with sepsis and impaired oxygenation. Serum from these patients disrupted endothelial architecture in vitro. This effect of septic serum was reversed by ANGPT-1, an ANGPT2 antagonist (Parikh, 2006).In a mouse model of ARDS induced by hemorrhagic shock, pretreatment of mice with anti-angpt2 antibodies significantly improved lung function, blood oxygenation, and survival (Lomas-Neira, 2016).

[0009] Increased vascular permeability (vascular hyperpermeability) contributes to numerous diseases, including ARDS, sepsis, severe sepsis, septic shock, cancer, and inflammation. Reduction of pulmonary vascular hyperpermeability reduces fluid accumulation in the alveolar spaces (pulmonary edema), thereby improving gas exchange between the lungs and blood vessels, resulting in better oxygenation of arterial blood. Improved oxygenation of arterial blood translates into better oxygenation of all organs (e.g., brain, heart, liver, kidneys), reducing the risk of multiple organ failure leading to death. Increased vascular permeability in sepsis, severe sepsis and septic shock has also been reported in several organs, such as the lungs, kidneys, liver and heart. Fluid accumulation in these organs impairs their proper function (resulting, for example, in arrhythmias, disruption of renal glomerular filtration or metabolic dysfunction) and leads to organ failure that can result in death. Pulmonary edema is a condition in which the lungs fill with fluid. The most common cause of pulmonary edema is congestive heart failure. Other less common conditions that may cause pulmonary edema include sudden high blood pressure, pneumonia, kidney failure, lung damage caused by severe infection, and blood poisoning caused by severe sepsis or infection. Acute lung injury (ALI) is a lung injury that often results from smoke inhalation, including recent use of e-cigarettes or vaping products.

[0010] Acute respiratory distress syndrome (ARDS) is a pulmonary inflammation characterized by increased pulmonary vascular permeability and / or pulmonary edema. ARDS is often characterized as low, moderate or severe based on the degree of hypoxemia. ARDS may be caused by several causes, for example, by bacterial or viral lung infection, induced by sepsis, inhalation of noxious substances, severe pneumonia, trauma, pancreatitis (inflammation of the pancreas), massive blood transfusion and burns. The most common cause of ARDS is sepsis. Severe acute respiratory syndrome (SARS) is a viral respiratory illness caused by a coronavirus called SARS-associated coronavirus (SARS-CoV). SARS begins with a high fever (body temperature above 100.4°F [>38.0°C]). Other symptoms may include sore throat, cough, headache, general discomfort, and body aches. Some people have mild respiratory symptoms initially. Most patients develop pneumonia. Until the SARS-CoV-2 pandemic outbreak from 2004 to December 2019, there had been no known cause of SARS reported worldwide. Middle East Respiratory Syndrome (MERS) is a disease caused by a virus (more specifically, a coronavirus) called Middle East Respiratory Syndrome Coronavirus (MERS-CoV). The disease is characterized by severe respiratory illness, including fever, cough, and shortness of breath. Approximately 3 or 4 deaths occur for every 10 patients reported to have MERS.

[0011] Sepsis, severe sepsis and septic shock are disorders resulting from a systemic inflammatory response to infection (see Mitchell M. Levy et al., Crit Care Med. 2003 Apr;31(4):1250-6). Sepsis is a disorder with both infection (e.g., viral, bacterial, abdominal trauma, intestinal perforation) and a systemic inflammatory response. This results in increased vascular permeability in several organs, such as the kidney, liver, heart and lungs. Severe sepsis (sepsis with organ dysfunction) refers to sepsis with acute organ dysfunction caused by sepsis. Septic shock refers to persistent hypotension with unexplained other causes. Therefore, there is a need for high-affinity neutralizing antibodies against ANGPT2 that limit antagonist binding of ANGPT2 to Tie2. Enhancement of Tie2 signaling is expected to have a number of beneficial effects, including, for example, stabilization of renal glomerular capillary structure, reduction of endothelial activity, and restoration of filtration barrier integrity. Overall, these beneficial effects are expected to reduce proteinuria, preserve renal function, and slow disease progression in patients with chronic kidney disease (CKD). The beneficial effects of high affinity neutralizing antibodies against ANGPT2 are further expected to aid in the treatment of patients afflicted with pulmonary vascular hyperpermeability and related disorders. Summary of the Invention

[0012] The present invention provides a monoclonal antibody that specifically binds to human ANGPT2.In one embodiment of the present invention, the antibody of the present invention neutralizes ANGPT2.Therefore, the antibody of the present invention is useful for treating and / or preventing diseases or disorders that can be alleviated by neutralizing ANGPT2, for example. In another aspect, the present invention provides anti-ANGPT2 antibodies, particularly humanized anti-ANGPT2 antibodies, having one or more properties described hereinbelow. In one embodiment, the present invention provides A heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 13 (H-CDR1); the amino acid sequence of SEQ ID NO: 15 (H-CDR2); and the amino acid sequence of SEQ ID NO: 17 (H-CDR3), and A light chain variable region comprising the amino acid sequence of SEQ ID NO:19 (L-CDR1); the amino acid sequence of SEQ ID NO:22 (L-CDR2); and the amino acid sequence of SEQ ID NO:24 (L-CDR3). or a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 14 (H-CDR1); the amino acid sequence of SEQ ID NO: 15 (H-CDR2); and the amino acid sequence of SEQ ID NO: 17 (H-CDR3); and A light chain variable region comprising the amino acid sequence of SEQ ID NO:19 (L-CDR1); the amino acid sequence of SEQ ID NO:22 (L-CDR2); and the amino acid sequence of SEQ ID NO:24 (L-CDR3). or a heavy chain variable region comprising the amino acid sequence of SEQ ID NO:13 (H-CDR1); the amino acid sequence of SEQ ID NO:15 (H-CDR2); and the amino acid sequence of SEQ ID NO:16 (H-CDR3); and A light chain variable region comprising the amino acid sequence of SEQ ID NO:20 (L-CDR1); the amino acid sequence of SEQ ID NO:23 (L-CDR2); and the amino acid sequence of SEQ ID NO:24 (L-CDR3). or a heavy chain variable region comprising the amino acid sequence of SEQ ID NO:13 (H-CDR1); the amino acid sequence of SEQ ID NO:15 (H-CDR2); and the amino acid sequence of SEQ ID NO:16 (H-CDR3); and A light chain variable region comprising the amino acid sequence of SEQ ID NO:21 (L-CDR1); the amino acid sequence of SEQ ID NO:23 (L-CDR2); and the amino acid sequence of SEQ ID NO:25 (L-CDR3). or a heavy chain variable region comprising the amino acid sequence of SEQ ID NO:13 (H-CDR1); the amino acid sequence of SEQ ID NO:15 (H-CDR2); and the amino acid sequence of SEQ ID NO:16 (H-CDR3); and A light chain variable region comprising the amino acid sequence of SEQ ID NO:20 (L-CDR1); the amino acid sequence of SEQ ID NO:22 (L-CDR2); and the amino acid sequence of SEQ ID NO:24 (L-CDR3). The present invention provides an anti-ANGPT2 antibody or antigen-binding fragment thereof, comprising:

[0013] In another embodiment, the present invention provides A variable heavy chain and a variable light chain comprising the amino acid sequences of SEQ ID NO:3 and SEQ ID NO:8, respectively; or A variable heavy chain and a variable light chain comprising the amino acid sequences of SEQ ID NO: 4 and SEQ ID NO: 9, respectively; or a variable heavy chain and a variable light chain comprising the amino acid sequences of SEQ ID NO:5 and SEQ ID NO:10, respectively; or a variable heavy chain and a variable light chain comprising the amino acid sequences of SEQ ID NO:6 and SEQ ID NO:11, respectively; or A variable heavy chain and a variable light chain comprising the amino acid sequences of SEQ ID NO: 7 and SEQ ID NO: 12, respectively; The present invention provides an anti-ANGPT2 antibody or antigen-binding fragment thereof comprising:

[0014] In another embodiment, the present invention provides A heavy chain comprising the amino acid sequence of SEQ ID NO:31 and a light chain comprising the amino acid sequence of SEQ ID NO:32 or A heavy chain comprising the amino acid sequence of SEQ ID NO:33 and a light chain comprising the amino acid sequence of SEQ ID NO:34 or A heavy chain comprising the amino acid sequence of SEQ ID NO:35 and a light chain comprising the amino acid sequence of SEQ ID NO:36 or A heavy chain comprising the amino acid sequence of SEQ ID NO:37 and a light chain comprising the amino acid sequence of SEQ ID NO:38 or A heavy chain comprising the amino acid sequence of SEQ ID NO:39 and a light chain comprising the amino acid sequence of SEQ ID NO:40 The present invention provides an anti-ANGPT2 antibody or antigen-binding fragment thereof comprising:

[0015] In one embodiment, the present invention provides a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 13 (H-CDR1); the amino acid sequence of SEQ ID NO: 15 (H-CDR2); and the amino acid sequence of SEQ ID NO: 17 (H-CDR3); or a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 14 (H-CDR1); the amino acid sequence of SEQ ID NO: 15 (H-CDR2); and the amino acid sequence of SEQ ID NO: 17 (H-CDR3); or a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 13 (H-CDR1); the amino acid sequence of SEQ ID NO: 15 (H-CDR2); and the amino acid sequence of SEQ ID NO: 16 (H-CDR3); or a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 13 (H-CDR1); the amino acid sequence of SEQ ID NO: 15 (H-CDR2); and the amino acid sequence of SEQ ID NO: 16 (H-CDR3); or A heavy chain variable region comprising the amino acid sequence of SEQ ID NO:13 (H-CDR1); the amino acid sequence of SEQ ID NO:15 (H-CDR2); and the amino acid sequence of SEQ ID NO:16 (H-CDR3). And A heavy chain framework region comprising one to four amino acid sequences selected from the group consisting of the amino acid sequence of SEQ ID NO: 41 (H-FR1); the amino acid sequence of SEQ ID NO: 42 (H-FR2); the amino acid sequence of SEQ ID NO: 43 (H-FR3); and the amino acid sequence of SEQ ID NO: 44 (H-FR4). The present invention provides an anti-ANGPT2 antibody or antigen-binding fragment thereof, comprising:

[0016] In another embodiment, the present invention provides a light chain variable region comprising the amino acid sequence of SEQ ID NO: 19 (L-CDR1); the amino acid sequence of SEQ ID NO: 22 (L-CDR2); and the amino acid sequence of SEQ ID NO: 24 (L-CDR3); or a light chain variable region comprising the amino acid sequence of SEQ ID NO: 19 (L-CDR1); the amino acid sequence of SEQ ID NO: 22 (L-CDR2); and the amino acid sequence of SEQ ID NO: 24 (L-CDR3); or A light chain variable region comprising the amino acid sequence of SEQ ID NO:20 (L-CDR1); the amino acid sequence of SEQ ID NO:23 (L-CDR2); and the amino acid sequence of SEQ ID NO:24 (L-CDR3); or a light chain variable region comprising the amino acid sequence of SEQ ID NO:21 (L-CDR1); the amino acid sequence of SEQ ID NO:23 (L-CDR2); and the amino acid sequence of SEQ ID NO:25 (L-CDR3); or a light chain variable region comprising the amino acid sequence of SEQ ID NO:20 (L-CDR1); the amino acid sequence of SEQ ID NO:22 (L-CDR2); and the amino acid sequence of SEQ ID NO:24 (L-CDR3); And A light chain framework region comprising one to four amino acid sequences selected from the group consisting of the amino acid sequence of SEQ ID NO: 45 (L-FR1); the amino acid sequence of SEQ ID NO: 46 (L-FR2); the amino acid sequence of SEQ ID NO: 47 (L-FR3); and the amino acid sequence of SEQ ID NO: 48 (L-FR4). The present invention provides an anti-ANGPT2 antibody or antigen-binding fragment thereof, comprising:

[0017] In one embodiment, the present invention provides an anti-ANGPT2 antibody or antigen-binding fragment thereof that binds to at least one amino acid residue within amino acid region 117 to 148 of the carboxy-terminal fibrinogen-like domain (FLD) region of human ANGPT2 having SEQ ID NO:50. In another embodiment, the invention relates to an ANGTP2 antibody or antigen-binding fragment thereof that binds to SEQ ID NO:51. In one embodiment, the anti-ANGPT2 antibody is a humanized anti-ANGPT2 antibody. In another embodiment, the anti-ANGPT2 antibody is a chimeric anti-ANGPT2 antibody.

[0018] In one embodiment, the invention provides an anti-ANGPT2 antibody or antigen-binding fragment thereof for use in medicine. In one embodiment, the invention provides an anti-ANGPT2 antibody or antigen-binding fragment thereof for use in treating renal disease. In one embodiment, the invention provides an anti-ANGPT2 antibody or antigen-binding fragment thereof for use in treating liver disease. In one embodiment, the invention provides a pharmaceutical composition comprising an anti-ANGPT2 antibody or antigen-binding fragment thereof and a pharma- ceutically acceptable carrier. In one embodiment, the invention provides an anti-ANGPT2 antibody or antigen-binding fragment thereof, or a pharmaceutical composition comprising an anti-ANGPT2 antibody or antigen-binding fragment thereof, wherein the antibody or antigen-binding fragment thereof is administered by a parenteral, intravenous, intravitreal, or subcutaneous route of administration. In one embodiment, the invention provides an isolated polynucleotide or polynucleotides comprising a sequence encoding a light chain or light chain variable region of an antibody or antigen-binding fragment thereof and a heavy chain or heavy chain variable region of an antibody or antigen-binding fragment thereof.

[0019] In one embodiment, the invention provides an expression vector comprising an isolated polynucleotide or multiple polynucleotides encoding a light chain or light chain variable region of an antibody or antigen-binding fragment thereof and a heavy chain or heavy chain variable region of an antibody or antigen-binding fragment thereof. In one embodiment, the invention provides a viral vector comprising an isolated polynucleotide or multiple polynucleotides encoding a light chain or light chain variable region of an antibody or antigen-binding fragment thereof and a heavy chain or heavy chain variable region of an antibody or antigen-binding fragment thereof. In one embodiment, the invention provides a host cell comprising an expression vector or an isolated polynucleotide or a plurality of polynucleotides encoding a light chain or light chain variable region of an antibody or antigen-binding fragment thereof and a heavy chain or heavy chain variable region of an antibody or antigen-binding fragment thereof. In one embodiment, the present invention provides 1. A method for producing an anti-ANGPT2 antibody or antigen-binding fragment thereof, comprising: Obtaining a host cell comprising an expression vector or an isolated polynucleotide or a plurality of polynucleotides encoding a light chain or a light chain variable region of an antibody or an antigen-binding fragment thereof and a heavy chain or a heavy chain variable region of an antibody or an antigen-binding fragment thereof; and culturing the host cell. The present invention provides a method comprising: In one embodiment, the method for producing an anti-ANGPT2 antibody or antigen-binding fragment thereof further comprises recovering and purifying the anti-ANGPT2 antibody or antigen-binding fragment thereof.

[0020] In another embodiment, the present invention provides An isolated polynucleotide or polynucleotides comprising a heavy chain as set forth in SEQ ID NO:31 or a sequence encoding a heavy chain variable region as set forth in SEQ ID NO:3; and a light chain as set forth in SEQ ID NO:32 or a sequence encoding a light chain variable region as set forth in SEQ ID NO:8. or An isolated polynucleotide or polynucleotides comprising a sequence encoding a heavy chain as set forth in SEQ ID NO:33 or a heavy chain variable region as set forth in SEQ ID NO:4; and a sequence encoding a light chain as set forth in SEQ ID NO:34 or a light chain variable region as set forth in SEQ ID NO:9. or An isolated polynucleotide or polynucleotides comprising a heavy chain as set forth in SEQ ID NO:35 or a sequence encoding a heavy chain variable region as set forth in SEQ ID NO:5; and a light chain as set forth in SEQ ID NO:36 or a sequence encoding a light chain variable region as set forth in SEQ ID NO:10. or An isolated polynucleotide or polynucleotides comprising a sequence encoding a heavy chain as set forth in SEQ ID NO:37 or a heavy chain variable region as set forth in SEQ ID NO:6; and a sequence encoding a light chain as set forth in SEQ ID NO:38 or a light chain variable region as set forth in SEQ ID NO:11. or An isolated polynucleotide or a plurality of polynucleotides comprising a sequence encoding a heavy chain as set forth in SEQ ID NO:39 or a heavy chain variable region as set forth in SEQ ID NO:7; and a sequence encoding a light chain as set forth in SEQ ID NO:40 or a light chain variable region as set forth in SEQ ID NO:12. Regarding.

[0021] Non-limiting examples of diseases, disorders, or conditions that may be alleviated by the anti-ANGPT2 antibodies of the invention include cardiac hypertrophy, myocardial infarction, ischemia, ischemic reperfusion injury, hypertensive stroke, pulmonary arterial hypertension, idiopathic pulmonary arterial hypertension, trauma-induced brain damage, asthma, chronic obstructive pulmonary disease (COPD), rheumatoid arthritis, inflammatory bowel disease, multiple sclerosis, preeclampsia and pregnancy-induced hypertension, sepsis, severe sepsis, septic shock, nonalcoholic steatohepatitis (NASH), cirrhosis, minimal change syndrome, focal segmental glomerulosclerosis (FSGS), nephrotic syndrome, diabetic nephropathy (DKD), chronic kidney disease (CKD), diabetic renal failure, end stage renal disease, ischemia or ischemic reperfusion injury, cancer, hepatocellular carcinoma, idiopathic pulmonary fibrosis (IPF), emphysema, acute lung injury (ALI), acute respiratory distress syndrome (ARDS), and the like. These include: Acute Respiratory Syndrome (ARDS), Severe Acute Respiratory Syndrome (SARS), Middle East Respiratory Syndrome (MERS), vascular hyperpermeability (and related disorders), acute kidney injury (AKI), renal cell carcinoma, heart failure, lupus nephritis, Raynaud's, pancreatitis, peripheral arterial disease, congenital heart disease, dengue virus, malaria, hantavirus, edema, regeneration, lupus, interstitial lung disease, scleroderma, retinopathy, diabetic nephropathy, portal hypertension, varices growth, and liver transplantation. [Brief description of the drawings]

[0022] [Figure 1A-1B] FIG. 1 shows the results of angiopoietin 2 cell-based complement-dependent cytotoxicity (CDC) assays (duplicate study) showing the cytotoxicity of ANGPT2-opt-13 (-□-), a REGN910 analog (nevescuumab) (-△-), a MEDI3617 analog (-◇-), and LC06 (-*-). [Diagram 2]FIG. 1 shows epitope mapping of anti-ANGPT2 antibodies: chimeric leads CL-209881, ANGPT2-opt-13, a nevescuumab analog, a MEDI3617 analog, and LC06 to the FLD domain (SEQ ID NO: 59). The specific binding site of each molecule to the extracellular FLD domain of human ANGTP2 (SEQ ID NO: 59) is highlighted in dark grey. [Figure 3A-3G] FIG. 3G shows the results of ANGPT2 blocking assays for ANGPT2-opt-1 (FIG. 3A), ANGPT2-opt-2 (FIG. 3B), ANGPT2-opt-13 (FIG. 3C), ANGPT2-opt-19 (FIG. 3D), ANGPT2-opt-31 (FIG. 3E), chimeric lead CL-209881 (FIG. 3F), and an analog of nevescuumab (FIG. 3G). DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0023] The general structure of antibodies or immunoglobulins is well known to those skilled in the art; these molecules are typically heterotetrameric glycoproteins of about 150,000 daltons, consisting of two identical light (L) chains and two identical heavy (H) chains. Each light chain is covalently linked to a heavy chain by one disulfide bond to form a heterodimer, and a heterotrimeric molecule is formed through covalent disulfide bonds between the two identical heavy chains of the heterodimer. The light and heavy chains are linked together by one disulfide bond, but the number of disulfide bonds between the two heavy chains varies depending on the immunoglobulin isotype. Each heavy and light chain also has regularly spaced intrachain disulfide bridges. Each heavy chain contains at its amino terminus a variable domain (V H = heavy chain variable region), followed by three or four constant domains (C H1 , C H2 , C H3 , and C H4 ), and C H1 and C. H2 Each light chain has two domains: an amino-terminal variable domain (V L = light chain variable region) and the carboxy-terminal constant domain (CL ) V L Domain is V H domain and is non-covalently associated with the C L The domains are usually linked together via disulfide bonds H1 The light-chain variable domain is covalently linked to the heavy-chain variable domain. Certain amino acid residues are thought to form an interface between the light-chain variable domain and the heavy-chain variable domain (Chothia et al., 1985, J. Mol. Biol. 186:651-663).

[0024] Certain domains within the variable domains vary widely among different antibodies, i.e., are "hypervariable". These hypervariable domains contain residues that are directly involved in the binding and specificity of each particular antibody to its specific antigenic determinant. The hypervariability in both the light and heavy variable domains is concentrated in three segments known as complementarity determining regions (CDRs) or hypervariable loops (HVLs). The CDRs are described in Kabat et al., 1991, In: Sequences of Proteins of Immunological Interest, 5 thThe CDRs are defined by comparison of sequences in the light chain variable domain, Ed. Public Health Service, National Institutes of Health, Bethesda, Md., whereas the HVLs are structurally defined according to the three-dimensional structure of the variable domain as described by Chothia and Lesk, 1987, J. Mol. Biol. 196: 901-917. Where the identification of CDRs resulting from these two methods differs slightly, the structural definition takes precedence. As defined by Kabat, CDR-L1 is located at about residues 24-34, CDR-L2 is located at about residues 50-56, and CDR-L3 is located at about residues 89-97 of the light chain variable domain. CDR-H1 is located at about residues 31-35, CDR-H2 is located at about residues 50-65, and CDR-H3 is located at about residues 95-102 of the heavy chain variable domain. The CDR1, CDR2, and CDR3 of the heavy and light chains thus define the specific, inherent and functional properties of a given antibody.

[0025] The three CDRs in each of the heavy and light chains are separated by framework regions (FRs), which tend to have sequences that are less variable. From the amino terminus to the carboxy terminus of the heavy and light chain variable domains, the FRs and CDRs are arranged in the following order: FR1, CDR1, FR2, CDR2, FR3, CDR3, and FR4. The largely β-sheet conformation of the FRs brings the CDRs in each of the chains into close proximity with each other and with the CDRs of the other chain. The resulting conformation is involved in the antigen-binding site (see Kabat et al., 1991, NIH Publ. No. 91-3242, Vol. I, pages 647-669), although not all CDR residues are directly involved in antigen binding. FR residues and Ig constant domains are not directly involved in antigen binding, but contribute to antigen binding and / or mediate antibody effector functions. It is believed that some FR residues have a significant impact on antigen binding in at least three ways: directly non-covalently binding to epitopes, interacting with one or more CDR residues, and influencing the interface between heavy and light chains. Constant domains are not directly involved in antigen binding, but mediate various Ig effector functions, such as antibody participation in antibody-dependent cellular cytotoxicity (ADCC), complement-dependent cytotoxicity (CDC) and antibody-dependent cellular phagocytosis (ADCP).

[0026] The light chains of vertebrate immunoglobulins are assigned to one of two clearly distinct classes, kappa (κ) and lambda (λ), based on the amino acid sequence of the constant domain. By comparison, the heavy chains of mammalian immunoglobulins are assigned to one of five major classes, IgA, IgD, IgE, IgG, and IgM, according to the sequence of the constant domain. IgG and IgA are further divided into subclasses (isotypes), e.g., IgG1, IgG2, IgG3, IgG4, IgA1, and IgA2, respectively. The heavy chain constant domains corresponding to the different classes of immunoglobulins are called α, δ, ε, γ, and μ, respectively. The subunit structures and three-dimensional configurations of the native immunoglobulin classes are well known. The terms "antibody," "anti-Angiopoietin 2 antibody," "anti-ANGPT2 antibody," "humanized anti-ANGPT2 antibody," and "variant humanized anti-ANGPT2 antibody" are used herein in the broadest sense and specifically encompass monoclonal antibodies (including full-length monoclonal antibodies), multispecific antibodies (e.g., bispecific antibodies), antibodies with minor modifications, such as N-terminal or C-terminal truncations, and antibody fragments, such as variable domains and other antibody portions, that exhibit the desired biological activity, e.g., ANGPT2 binding.

[0027] The term "monoclonal antibody" (mAb) refers to an antibody from a population of substantially homogeneous antibodies; that is, the individual antibodies in the population are identical except for naturally occurring mutations that may be present in minor amounts. Monoclonal antibodies are highly specific, being directed against a single antigenic determinant, "epitope." Thus, the modifier "monoclonal" indicates a substantially homogeneous population of antibodies directed against the same epitope, and is not to be construed as requiring production of the antibodies by any particular method. It should be understood that the monoclonal antibodies can be made by any technique or methodology known in the art, including, for example, the hybridoma method (Kohler et al., 1975, Nature 256:495), or recombinant DNA methods known in the art (see, e.g., U.S. Pat. No. 4,816,567), or methods for isolating recombinantly produced monoclonals using phage antibody libraries using the techniques described in Clackson et al., 1991, Nature 352: 624-628, and Marks et al., 1991, J. Mol. Biol. 222: 581-597.

[0028] A chimeric antibody consists of the heavy and light chain variable regions of an antibody from one species (e.g., a non-human mammal such as a mouse) and the heavy and light chain constant regions of an antibody from another species (e.g., human), and can be obtained by linking a DNA sequence encoding the variable region of an antibody from a first species (e.g., a mouse) to a DNA sequence of the constant region of an antibody from a second species (e.g., human) and transforming a host with an expression vector carrying the linked sequences, thereby enabling the host to produce the chimeric antibody. Alternatively, a chimeric antibody can also be an antibody in which one or more regions or domains of the heavy and / or light chain are identical to, homologous to, or variants of corresponding sequences in a monoclonal antibody from another immunoglobulin class or isotype, or from a consensus or germline sequence. Chimeric antibodies can include antibody fragments so long as such antibody fragments exhibit the desired biological activity of the parent antibody, e.g., binding to the same epitope (see, e.g., U.S. Pat. No. 4,816,567, and Morrison et al., 1984, Proc. Natl. Acad. Sci. USA 81: 6851-6855).

[0029] The terms "antibody fragment", "antigen-binding fragment", "anti-ANGPT2 antibody fragment", "humanized anti-ANGPT2 antibody fragment", "variant humanized anti-ANGPT2 antibody fragment" refer to a portion of a full-length anti-ANGPT2 antibody that retains variable regions or functional capabilities, such as specific ANGPT2 epitope binding. Examples of antibody fragments include, but are not limited to, Fab fragments, Fab' fragments, F(ab')2 fragments, Fd fragments, Fv fragments, scFv fragments, and scFv-Fc fragments, diabodies, linear antibodies, single-chain antibodies, minibodies, diabodies formed from antibody fragments, and multispecific antibodies formed from antibody fragments. Full-length antibodies can be treated with enzymes such as papain or pepsin to generate useful antibody fragments. Papain digestion produces two identical antigen-binding antibody fragments, called "Fab" fragments, each with a single antigen-binding site, and a residual "Fc" fragment. The Fab fragment also contains the constant domains of the light chain and the C domain of the heavy chain. H1 Pepsin treatment yields an F(ab')2 fragment that has two antigen-binding sites and is still capable of cross-linking antigen.

[0030] The Fab' fragment is H1 They differ from Fab fragments by the presence of additional residues, including one or more cysteines, in the antibody hinge region at the C-terminus of the domain. F(ab')2 antibody fragments are pairs of Fab' fragments linked by cysteine ​​residues in the hinge region. Other chemical bonds of antibody fragments are also known. An "Fv" fragment contains a complete antigen recognition and binding site, consisting of a dimer of one heavy- and one light-chain variable domain in tight, non-covalent association. In this configuration, the three CDRs of each variable domain interact to form a V H -V L Collectively, the six CDRs confer antigen-binding specificity to the antibody, defining an antigen-binding site on the surface of the dimer. "Single-chain Fv" or "scFv" antibody fragments are fragments of the V H Domains and V L A single chain Fv variant is one that contains V domains, where these domains are present in a single polypeptide chain. Single chain Fvs can recognize and bind antigens. scFv polypeptides contain V domains that facilitate the formation of the desired three-dimensional structure for antigen binding by the scFv. H Domain and V LThere may also be an optional polypeptide linker located between the domains (see, e.g., Pluckthun, 1994, In The Pharmacology of Monoclonal Antibodies, Vol. 113, Rosenburg and Moore eds., Springer-Verlag, New York, pp. 269-315).

[0031] Other recognized antibody fragments include those that contain tandem Fd segments (V H -C H1 -V H -C H1 ) pairs. These "linear antibodies" can be bispecific or monospecific, e.g., as described in Zapata et al. 1995, Protein Eng. 8(10):1057-1062. A humanized antibody or humanized antibody fragment is a specific type of chimeric antibody, which includes an immunoglobulin amino acid sequence variant or fragment thereof that can bind to a given antigen and contains one or more FRs that have substantially the amino acid sequence of a human immunoglobulin and one or more CDRs that have substantially the amino acid sequence of a non-human immunoglobulin. This non-human amino acid sequence, often called the "import" sequence, typically comes from an "import" antibody domain, particularly the variable domain. Usually, a humanized antibody contains at least the CDRs or HVLs of a non-human antibody inserted between the FRs of a human heavy or light chain variable domain. The present invention describes a specific humanized anti-ANGPT2 antibody with CDRs derived from the chimeric lead CL-209881 inserted between the FRs of human germline sequence heavy and light chain variable domains.

[0032] In one embodiment, the humanized anti-ANGPT2 antibody comprises at least one, and typically substantially all of two, variable domains (e.g., contained in Fab fragments, Fab' fragments, F(ab')2 fragments, Fabc fragments, and Fv fragments), with all or substantially all of the CDRs corresponding to the CDRs of a non-human immunoglobulin, and specifically herein, the CDRs are the murine sequences of the chimeric lead CL-209881, and the FRs are the consensus or germline sequence FRs of a human immunoglobulin. In another embodiment, the humanized anti-ANGPT2 antibody also comprises at least a portion of an immunoglobulin Fc region, typically the Fc region of a human immunoglobulin. Typically, the antibody has both a light chain and at least the variable domain of a heavy chain. The antibody also optionally comprises the CDRs of the heavy chain. H1 Area, hinge area, C H2 area, C H3 Area, and / or C H4 It may include one or more of the regions. The humanized anti-ANGPT2 antibody can be selected from any class of immunoglobulin, including IgM, IgG, IgD, IgA, and IgE, and any isotype, including IgG1, IgG2, IgG3, IgG4, IgA1, and IgA2. For example, the constant domain can be a complement fixing constant domain when it is desired that the humanized antibody exhibit cytotoxic activity, and the isotype is typically IgG1. When such cytotoxic activity is not desired, the constant domain can be of another isotype, such as IgG2. Alternative humanized anti-ANGPT2 antibodies can include sequences from more than one immunoglobulin class or isotype, and the selection of a particular constant domain to optimize the desired effector function is within the ordinary skill in the art.

[0033] The FR and CDR or HVL of humanized anti-ANGPT2 antibody need not correspond exactly to the parent sequence. For example, one or more residues in imported CDR or HVL, or consensus or germline FR sequence, may be modified (e.g., mutagenized) by substitution, insertion, or deletion, such that the resulting amino acid residue is no longer identical to the original residue at the corresponding position in any parent sequence, but the antibody still retains the binding function to ANGPT2. Such modifications are typically conservative modifications, not major. Usually, at least 75% of the humanized antibody residues, more frequently at least 90%, and most frequently more than 95%, or more than 98%, or more than 99% correspond to the residues of parent consensus or germline FR and imported CDR sequence. The interface between the heavy chain variable region and the light chain variable region ("V L -V H Immunoglobulin residues that affect the "interface" are those that affect the proximity or orientation of the two chains with respect to one another. Certain residues that may be involved in interactions between the chains include the V L Residues 34, 36, 38, 44, 46, 87, 89, 91, 96, and 98, and V H Residues 35, 37, 39, 45, 47, 91, 93, 95, 100, and 103 (utilizing the numbering system set out in Kabat et al., Sequences of Proteins of Immunological Interest (National Institutes of Health, Bethesda, Md., 1987)) are included. U.S. Pat. No. 6,407,213 also discloses that V L Residues 43 and 85, and V H It is argued that residues such as residues 43 and 60 may also be involved in this interaction. Although these residues are shown for human IgG only, they are applicable across species. Key antibody residues that are reasonably expected to be involved in interchain interactions are selected for substitution into the consensus sequence.

[0034] The terms "consensus sequence" and "consensus antibody" refer to an amino acid sequence that contains the most frequently occurring amino acid residue at each position in all immunoglobulins of any particular class, isotype, or subunit structure, for example, in the human immunoglobulin variable domain. The consensus sequence may be based on immunoglobulins of a particular species or many species. It is understood that a "consensus" sequence, structure, or antibody encompasses the consensus human sequence as described in certain embodiments, and refers to an amino acid sequence that contains the most frequently occurring amino acid residue at each position in all human immunoglobulins of any particular class, isotype, or subunit structure. Thus, a consensus sequence has an amino acid sequence that has an amino acid at each position that is present in one or more known immunoglobulins, but may not be an exact duplicate of the entire amino acid sequence of any single immunoglobulin. The consensus sequence of the variable region is not derived from any naturally occurring antibody or immunoglobulin. Kabat et al., 1991, Sequences of Proteins of Immunological Interest, 5th Ed. Public Health Service, National Institutes of Health, Bethesda, Md. The FRs of the consensus sequences of heavy and light chains and their variants provide sequences useful for preparing humanized anti-ANGPT2 antibodies.See, for example, U.S. Patent No. 6,037,454 and U.S. Patent No. 6,054,297.

[0035] Human germline sequences are naturally found in the human population. The combination of these germline genes results in antibody diversity. Germline antibody sequences for antibody light chains are derived from conserved human germline kappa or lambda v and j genes. Similarly, heavy chain sequences are derived from germline v, d, and j genes (LeFranc, MP, and LeFranc, G, "The Immunoglobulin Facts Book" Academic Press, 2001). An "isolated" antibody is one that has been identified and separated and / or recovered from a component of its natural environment. Contaminant components of the antibody's natural environment are contaminants that may interfere with diagnostic or therapeutic uses of the antibody, and may be enzymes, hormones, or other proteinaceous or nonproteinaceous solutes. In one embodiment, the antibody is purified to at least greater than 95% isolation by weight of the antibody.

[0036] Isolated antibody includes the antibody in situ within a recombinant cell in which it is produced, since at least one component of the antibody's natural environment will not be present. Ordinarily, however, isolated antibody will be prepared by at least one purification step in which recombinant cell materials are removed. The term "antibody performance" refers to factors / characteristics that contribute to an antibody's antigen recognition or efficacy in vivo. Changes in the amino acid sequence of an antibody can affect antibody properties such as folding and can also affect the initial antibody binding rate to the antigen (k a ), the dissociation constant of the antibody from the antigen (k d ), the affinity constant of the antibody for the antigen (K d ), which can affect physical factors such as antibody conformation, protein stability, and antibody half-life. The term "neutralizing antibody" or "blocking antibody" refers to an antibody whose binding to ANGPT2 blocks the interaction between ANGPT2 and its receptor (Tie-2) and / or causes the inhibition of at least one biological function of ANGPT2.It will be understood that the inhibition caused by neutralizing or blocking antibody of ANGPT2 does not have to be complete, as long as it can be detected using suitable assay.The typical assay for detecting ANGPT2 inhibition is described herein or known in the art.

[0037] As used herein, the term "identical" or "percent identity" in the context of two or more nucleic acid or polypeptide sequences refers to two or more sequences or subsequences that are identical or have a certain percentage of identical nucleotides or amino acid residues when compared and aligned for maximum correspondence. To determine percent identity, the sequences are aligned for optimal comparison (e.g., gaps can be introduced into the sequence of the first amino acid or nucleic acid sequence for optimal alignment with the second amino acid or nucleic acid sequence). The amino acid residues or nucleotides at corresponding amino acid or nucleotide positions are then compared. If a position in the first sequence has an identical amino acid residue or nucleotide as the corresponding position in the second sequence, then the molecules are identical at that position. The percent identity between two sequences is a function of the number of identical positions shared by the sequences (i.e., % identity = number of identical positions / total number of positions (e.g., overlapping positions) x 100). In some embodiments, the two sequences to be compared are the same length (e.g., excluding additional sequences that extend beyond the sequences to be compared), after introducing gaps into the sequences as necessary. For example, when comparing variable region sequences, leader sequences and / or constant domain sequences are not considered. In sequence comparison between two sequences, "corresponding" CDRs refer to the CDRs that are located at the same position in both sequences (e.g., CDR-H1 of each sequence).

[0038] The determination of percent identity or similarity between two sequences can be carried out using a mathematical algorithm.A preferred non-limiting example of the mathematical algorithm used to compare two sequences is the algorithm of Karlin and Altschul, 1990, Proc. Natl. Acad. Sci. USA 87:2264-2268, modified as in Karlin and Altschul, 1993, Proc. Natl. Acad. Sci. USA 90:5873-5877.Such an algorithm is incorporated into the NBLAST and XBLAST programs of Altschul et al., 1990, J. Mol. Biol. 215:403-410.BLAST nucleotide searches can be carried out using the NBLAST program with score=100 and wordlength=12 to obtain nucleotide sequences homologous to the nucleic acid encoding the protein of interest. To obtain amino acid sequences homologous to a protein of interest, BLAST protein searches can be performed using the XBLAST program with score=50 and wordlength=3. To obtain gapped alignments for comparison purposes, Gapped BLAST can be utilized as described in Altschul et al., 1997, Nucleic Acids Res. 25:3389-3402. Alternatively, PSI-Blast can be used to perform an iterative search that detects distant relationships between molecules (Id.). When utilizing the BLAST, Gapped BLAST, and PSI-Blast programs, the default parameters of the respective programs (e.g., XBLAST and NBLAST) can be used. Another preferred, non-limiting example of a mathematical algorithm utilized for sequence comparison is the algorithm of Myers and Miller, CABIOS (1989). Such an algorithm is incorporated in the ALIGN program (version 2.0), which is part of the GCG sequence alignment software package.When utilizing the ALIGN program for comparison of amino acid sequences, a PAM120 weighted residue table, a gap length penalty of 12, and a gap penalty of 4 can be used. Additional algorithms for sequence analysis are known in the art, including ADVANCE and ADAM, described in Torellis and Robotti, 1994, Comput. Appl. Biosci. 10:3-5, and FASTA, described in Pearson and Lipman, 1988, Proc. Natl. Acad. Sci. USA 85:2444-8. Within FASTA, ktup is a control option that sets the sensitivity and speed of the search. If ktup=2, similar regions in the two sequences being compared are found by looking at aligned residue pairs, and if ktup=1, single aligned amino acids are examined. ktup can be set to 2 or 1 for protein sequences, or 1-6 for DNA sequences. If ktup is not specified, the default is 2 for proteins and 6 for DNA. Alternatively, protein sequence alignments may be performed using the CLUSTAL W algorithm as described by Higgins et al., 1996, Methods Enzymol. 266:383-402.

[0039] A nucleic acid sequence is "operably linked" when it is placed into a functional relationship with another nucleic acid sequence. For example, a nucleic acid precursor sequence or secretory leader is operably linked to a nucleic acid encoding a polypeptide if it is expressed as a preprotein that participates in the secretion of the polypeptide. A promoter or enhancer is operably linked to a coding sequence if it affects the transcription of the sequence. Or, a ribosome binding site is operably linked to a coding sequence if it is positioned so as to facilitate translation. Generally, "operably linked" means that the DNA sequences being linked are contiguous, and in the case of a secretory leader, contiguous and in reading frame. Enhancers, however, are optionally contiguous. Linking can be accomplished by ligation at convenient restriction sites. If such sites do not exist, synthetic oligonucleotide adaptors or linkers can be used.

[0040] As used herein, the expressions "cell," "cell line," and "cell culture" are used interchangeably and all such designations include the progeny thereof. Thus, "transformants" and "transformed cells" include the primary subject cell and cultures derived therefrom without regard for the number of transfers. The term "mammal" for purposes of treatment refers to any animal classified as a mammal, including humans, domestic and farm animals, and zoo, sport, or pet animals, such as dogs, horses, cats, cows, and the like. Preferably, the mammal is a human. A "disorder," as used herein, is any condition that would benefit from treatment with a humanized anti-ANGPT2 antibody described herein. This includes chronic and acute disorders or diseases, including pathological conditions that predispose a mammal to the disorder in question.

[0041] As used herein, the term "ANGPT2-associated disorder" or "ANGPT2-associated disease" refers to a condition in which modification or activation of cells expressing ANGPT2 is demonstrated. ANGPT2 associated disorders include diseases and disorders such as age-related macular degeneration, geographic atrophy, diabetic retinopathy, diabetic macular edema, retinitis pigmentosa, hereditary retinal dystrophies, hereditary macular dystrophies, myopic degeneration, retinal vein occlusion, retinal artery occlusion, endophthalmitis, uveitis, cystoid macular edema, choroidal neovascular membrane secondary to any retinal disease, optic neuropathy, glaucoma, retinal detachment, toxic retinopathy, radiation retinopathy, and traumatic retinopathy, as well as pre-symptomatic and mild to moderate Alzheimer's disease, delaying disease progression in patients with Alzheimer's disease, Huntington's disease, Parkinson's disease, major depressive disorder, schizophrenia, cognitive impairment with schizophrenia, preventing first episode psychosis in individuals with attenuated psychotic syndrome, preventing relapse in patients with schizophrenia, treatment-resistant depression, and metabolic diseases like hyperphagia, obesity, or metabolic syndrome.

[0042] ANGPT2-associated disorders also include cardiac hypertrophy, myocardial infarction, ischemia, ischemia-reperfusion injury, hypertensive stroke, pulmonary arterial hypertension, idiopathic pulmonary arterial hypertension, trauma-induced encephalopathy, asthma, chronic obstructive pulmonary disease (COPD), rheumatoid arthritis, inflammatory bowel disease, multiple sclerosis, preeclampsia and gestational hypertension, sepsis, severe sepsis, septic shock, nonalcoholic steatohepatitis (NASH), cirrhosis, minimal change syndrome, focal segmental glomerulosclerosis (FSGS), nephrotic syndrome, diabetic kidney disease (DKD), chronic kidney disease (CKD), diabetic renal failure, peripheral vascular disease (CVD), and peripheral vascular endothelial cell carcinoma (VEDV). Stage 1 renal disease, ischemia or ischemic reperfusion injury, cancer, hepatocellular carcinoma, idiopathic pulmonary fibrosis (IPF), emphysema, acute lung injury (ALI), acute respiratory distress syndrome (ARDS), severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), vascular hyperpermeability (and related disorders), acute kidney injury, renal cell carcinoma, heart failure, lupus nephritis, Raynaud's, pancreatitis, peripheral arterial disease, congenital heart disease, dengue virus, malaria, hantavirus, edema, regeneration, lupus, interstitial lung disease, scleroderma, retinopathy, diabetic nephropathy, portal hypertension, varicose vein development, and liver transplantation. The term "intravitreal injection" has its ordinary meaning in the art and refers to the introduction of an anti-ANGPT2 antibody, or antigen-binding fragment thereof, into the vitreous of a patient.

[0043] The term "specifically bind" or similar terms means that the anti-ANGPT2 antibody or its antigen-binding fragment forms a complex with the antigen that is relatively stable under physiological conditions. Methods for determining whether two molecules specifically bind are described herein or known in the art, including, for example, equilibrium dialysis, surface plasmon resonance, and the like. In one embodiment, specific binding is about 1×10 6 according to the affinity binding method described in the Examples section herein. -8 K below M D In another embodiment, specific binding is characterized by about 1×10 -9 K below M D An isolated antibody that specifically binds human Ang-2 may, however, have cross-reactivity to other antigens, such as ANGPT2-2 molecules from other species. Moreover, the isolated antibody may be substantially free of other cellular material and / or chemicals. The term "subcutaneous administration" refers to the introduction of an anti-ANGPT2 antibody or antigen-binding fragment thereof under the skin of an animal or human patient by relatively slow, sustained delivery from a drug reservoir, preferably into a pocket between the skin and the underlying tissue. The pocket can be created by pinching or pulling the skin up and away from the underlying tissue.

[0044] The term "subcutaneous infusion" refers to the introduction of a drug under the skin of an animal or human patient, preferably into the pocket between the skin and the underlying tissue, by relatively slow, sustained delivery from a drug container over a period of time, including but not limited to 30 minutes or less or 90 minutes or less.Optionally, the infusion may be performed by subcutaneous implantation of a drug delivery pump implanted under the skin of an animal or human patient, where the pump delivers a predetermined amount of drug over a predetermined period of time, for example 30 minutes, 90 minutes, or the length of a treatment regimen. The term "subcutaneous bolus" refers to drug administration beneath the skin of an animal or human patient, where the bolus drug delivery is less than approximately 15 minutes, in other embodiments less than 5 minutes, and in still other embodiments less than 60 seconds. In still other embodiments, administration occurs within a pocket between the skin and the underlying tissue, where the pocket can be created by pinching or pulling the skin up and away from the underlying tissue.

[0045] The term "therapeutically effective amount" is used to refer to the amount of anti-ANGPT2 antibody or its antigen-binding fragment that alleviates or improves one or more of the symptoms of the disorder to be treated. In alleviating or improving, the therapeutically effective amount is an amount that results in a favorable outcome for the patient. In one embodiment, the therapeutically effective amount has a neuroprotective or neuroregenerative effect. In another embodiment, the therapeutically effective amount refers to a target serum concentration that has been shown to be effective, for example, in delaying disease progression. Efficacy can be measured in a conventional manner, depending on the condition to be treated. For example, efficacy can be measured by determining the response rate, for example, vision recovery, or by evaluating the delay time to disease progression. The terms "treatment" and "therapy" and the like, as used herein, are meant to include therapeutic and preventive or suppressive measures for a disease or disorder that produce any clinically desirable or beneficial effect, including, but not limited to, alleviating or alleviating one or more symptoms, reversing, slowing or stopping the progression of a disease or disorder. Thus, for example, the term treatment includes administering an anti-ANGPT2 antibody or an antigen-binding fragment thereof before or after the onset of symptoms of a disease or disorder, thereby preventing or eliminating one or more symptoms of a disease or disorder. As another example, the term includes administering an anti-ANGPT2 antibody or an antigen-binding fragment thereof after clinical signs of a disease to combat symptoms of a disease. Furthermore, administration of an anti-ANGPT2 antibody or an antigen-binding fragment thereof after onset and after the appearance of clinical symptoms includes "treatment" or "therapy" as used herein, if the administration affects clinical parameters of the disease or disorder, such as the degree of tissue damage or the amount or extent of metastasis, whether or not the treatment results in improvement of the disease. Furthermore, so long as a composition of the present invention, alone or in combination with another therapeutic agent, reduces or ameliorates at least one symptom of the disorder being treated compared to that symptom in the absence of use of the anti-ANGPT2 antibody composition or antigen-binding fragment thereof, the result should be considered to be an effective treatment of the underlying disorder, whether or not all symptoms of the disorder are reduced. The term "package insert" is used to refer to instructions customarily included in commercial packages of therapeutic agents having information about the indications, methods of use, methods of administration, contraindications, and / or warnings concerning the use of such therapeutic agents.

[0046] antibody Herein, anti-ANGPT2 antibody, particularly humanized anti-ANGPT2 antibody, and compositions and products comprising the anti-ANGPT2 antibody of the present invention are described and disclosed.Anti-ANGPT2 antibody antigen-binding fragments are also described.Anti-ANGPT2 antibody and its antigen-binding fragments can be used in the treatment of various diseases or disorders characterized by reducing the activity of ANGPT2 pathway.Anti-ANGPT2 antibody and its antigen-binding fragments each comprise at least a portion that specifically recognizes ANGPT2 epitope. In the initial characterization, the anti-ANGPT2 chimeric lead CL-209881 was selected based on its superior antibody performance. The CDRs of the chimeric lead were placed into the FRs of human consensus heavy and light chain variable domains, and a library of variants was generated by further engineering the FRs with different modifications. This resulted in 33 sequences which were further optimized and problem-solved to yield six final candidates with enhanced properties, which are disclosed herein. The sequences of the antibodies of the invention are shown as follows:

[0047] VH sequence CL-209881_VH (chimeric lead), heavy chain variable region, SEQ ID NO:1 QVQLKQSGAELVKPGSSVKISCRAS GYIFIDYFIN WVKQRPGQGLEWIG KIGPGSGSSSSNEKFKG KATLTADKSSSTAYMQLSSLTSEDSAVYFCAR EAFDYDGDYYGMAY WGQGTSVTVSS ANGPT2-opt-1 (humanized) heavy chain variable region, SEQ ID NO:3 QVQLVQSGAEVKKPGSSVKVSCKAS GYIFIDYFIN WVRQAPGQGLEWMG KIGPGSGSSSSNEKFKG RVTITADKSTSTAYMELSSLRSEDTAVYYCAR EAFDYEGDYYGMAY WGQGTLVTVSS

[0048] ANGPT2-opt-2 (humanized) heavy chain variable region, SEQ ID NO:4 QVQLVQSGAEVKKPGSSVKVSCKAS GYIFIEYFIN WVRQAPGQGLEWMG KIGPGSGSSSSNEKFKG RVTITADKSTSTAYMELSSLRSEDTAVYYCAR EAFDYEGDYYGMAY WGQGTLVTVSS ANGPT2-opt-13 (humanized) heavy chain variable region, SEQ ID NO:5 QVQLVQSGAEVKKPGSSVKVSCKAS GYIFIDYFIN WVRQAPGQGLEWMG KIGPGSGSSSSNEKFKG RVTITADKSTSTAYMELSSLRSEDTAVYYCAR EAFDYDGDYYGMAY WGQGTLVTVSS ANGPT2-opt-19 (humanized) heavy chain variable region, SEQ ID NO:6 QVQLVQSGAEVKKPGSSVKVSCKAS GYIFIDYFIN WVRQAPGQGLEWMG KIGPGSGSSSSNEKFKG RVTITADKSTSTAYMELSSLRSEDTAVYYCAR EAFDYDGDYYGMAY WGQGTLVTVSS ANGPT2-opt-31 (humanized) heavy chain variable region, SEQ ID NO:7 QVQLVQSGAEVKKPGSSVKVSCKAS GYIFIDYFIN WVRQAPGQGLEWMG KIGPGSGSSSSNEKFKG RVTITADKSTSTAYMELSSLRSEDTAVYYCAR EAFDYDGDYYGMAY WGQGTLVTVSS

[0049] VL sequence CL-209881_VL (chimeric lead), light chain variable region, SEQ ID NO:2 DIVMTQSPSSLSVSAGEKVTMSC KSSQSLLNSGNQKNFLA WYQQKPGQPPKLLIY GASTRES GVPDRFTGSGSGTDFLTITSVQAEDLAVYYC QNDHSYPIT FGSGTKLEIK ANGPT2-opt-1 (humanized) light chain variable region, SEQ ID NO:8 EIVMTQSPATLSVSPGERATLSC KSSQSLLASGNQKNFLA WYQQKPGQAPRLLIY GASTRES GIPARFSGSGSGTEFTLTISSLQSEDFAVYYC QNDHSYPIT FGQGTKLEIK ANGPT2-opt-2 (humanized) light chain variable region, SEQ ID NO:9 EIVMTQSPATLSVSPGERATLSC KSSQSLLASGNQKNFLA WYQQKPGQAPRLLIY GASTRES GIPARFSGSGSGTEFTLTISSLQSEDFAVYYC QNDHSYPIT FGQGTKLEIK

[0050] ANGPT2-opt-13 (humanized) light chain variable region, SEQ ID NO: 10 EIVMTQSPATLSVSPGERATLSC KSSQSLLSSGNQKSFLA WYQQKPGQAPRLLIY GASTRET GIPARFSGSGSGTEFTLTISSLQSEDFAVYYC QNDHSYPIT FGQGTKLEIK ANGPT2-opt-19 (humanized) light chain variable region, SEQ ID NO: 11 EIVMTQSPATLSVSPGERATLSC RASQSVLSSGNQKSFLA WYQQKPGQAPRLLIY GASTRET GIPARFSGSGSGTEFTLTISSLQSEDFAVYYC QQDHSYPIT FGQGTKLEIK ANGPT2-opt-31 (humanized) light chain variable region, SEQ ID NO: 12 EIVMTQSPATLSVSPGERATLSC KSSQSLLSSGNQKSFLA WYQQKPGQAPRLLIY GASTRES GIPARFSGSGSGTEFTLTISSLQSEDFAVYYC QNDHSYPIT FGQGTKLEIK The underlined portions of the above sequences correspond to the CDR regions of the light and heavy chain variable regions.

[0051] The humanized anti-ANGPT2 antibodies of the present invention are antibodies having the light chain and heavy chain sequences shown in the table below. [Table 1] TIFF0007649756000002.tif208156 TIFF0007649756000003.tif109152

[0052] [Table 2] TIFF0007649756000005.tif130148

[0053] The CDRs above according to the Chemical Computing Group (CCG) numbering are underlined (Almagro et al., Proteins 2011; 79:3050-3066, and Maier et al, Proteins 2014; 82:1599-1610). Kabat sequence numbering is indicated by bold and Chothia numbering system is indicated by italic letters.

[0054] Humanized and amino acid sequence variants Further variant ANGPT2 antibodies and antibody fragments can be engineered based on the set of CDRs shown in SEQ ID NOs: 13-25. It is understood that in variant ANGPT2 antibodies and antibody fragments, the amino acid sequences of the CDRs remain unchanged, but the surrounding regions, e.g., FR regions, may be engineered. Amino acid sequence variants of anti-ANGPT2 antibodies can be prepared by introducing appropriate nucleotide changes into the DNA of the anti-ANGPT2 antibody or by peptide synthesis. Such variants include, for example, deletions from, and / or insertions into, and / or substitutions of, residues within the amino acid sequences of the anti-ANGPT2 antibodies of the examples herein. Any combination of deletions, insertions, and substitutions can be made to arrive at the final construct, so long as the final construct has the desired characteristics. Amino acid changes can also modify post-translational processing of the humanized or variant anti-ANGPT2 antibodies, e.g., changing the number or position of glycosylation sites.

[0055] In some embodiments, the present invention includes ANGPT2 antibodies or antibody fragments thereof having a heavy chain variable region and a light chain variable region, wherein the amino acid sequences of the heavy chain variable region and the light chain variable region are at least 80%, at least 90%, at least 95%, at least 98%, or at least 99% identical to the amino acid sequences of SEQ ID NO: 3 or 8, or 4 or 9, or 5 or 10, or 6 or 11, or 7 or 12, respectively.

[0056] In some embodiments, the invention includes anti-ANGPT2 antibodies or antibody fragments thereof having a heavy chain and a light chain, wherein the heavy chain amino acid sequence and the light chain amino acid sequence are at least 80%, at least 90%, at least 95%, at least 98%, or at least 99% identical to the amino acid sequences of SEQ ID NO: 31 or 32, or 33 or 34, or 35 or 36, or 37 or 38, or 39 or 40, respectively. Another type of amino acid variant of an antibody involves altering the original glycosylation pattern of the antibody, the term "altering" in this context meaning deleting one or more carbohydrate moieties found in the antibody and / or adding one or more glycosylation sites that were not previously present in the antibody. In some aspects, the present invention comprises the nucleic acid molecule encoding the amino acid sequence variant of anti-ANGPT2 antibody described herein.The nucleic acid molecule encoding the amino acid sequence variant of anti-ANGPT2 antibody is prepared by various methods known in the art.These methods include, but are not limited to, isolation from natural sources (in the case of naturally occurring amino acid sequence variants), or preparation of pre-prepared variant or non-variant version of anti-ANGPT2 antibody by oligonucleotide-mediated (or site-directed) mutagenesis, PCR mutagenesis, and cassette mutagenesis.

[0057] In certain embodiments, the anti-ANGPT2 antibody is an antibody fragment. There are techniques developed for the production of antibody fragments. Fragments can be derived through proteolytic digestion of intact antibodies (see, for example, Morimoto et al., 1992, Journal of Biochemical and Biophysical Methods 24:107-117, and Brennan et al., 1985, Science 229:81). Alternatively, fragments can be produced directly in recombinant host cells. For example, Fab'-SH fragments can be directly recovered from E. coli and chemically coupled to form F(ab')2 fragments (see, for example, Carter et al., 1992, Bio / Technology 10:163-167). By another approach, F(ab')2 fragments can be directly isolated from recombinant host cell culture. Other techniques for producing antibody fragments will be apparent to those skilled in the art. The anti-ANGPT2 antibodies and antigen-binding fragments thereof can include modifications.

[0058] In certain embodiments, it may be desirable to use anti-ANGPT2 antibody fragments rather than intact antibodies. It may be desirable to modify antibody fragments to increase their serum half-life. This can be done, for example, by incorporating salvage receptor binding epitopes into the antibody fragment. In one method, the appropriate region of the antibody fragment can be modified (e.g., mutated), or the epitope can be incorporated into a peptide tag, which can then be fused to either end or the middle of the antibody fragment, for example, by DNA synthesis or peptide synthesis. For example, see WO 96 / 32478. In other embodiments, the present invention includes covalent modifications of anti-ANGPT2 antibodies. Covalent modifications include modifications of cysteinyl, histidyl, lysinyl and amino-terminal residues, arginyl, tyrosyl, carboxyl side groups (aspartyl or glutamyl), glutaminyl and asparaginyl residues, or seryl or threonyl residues. Another type of covalent modification involves chemically or enzymatically binding glycosides to the antibody. Such modifications can be performed by chemical synthesis or, where appropriate, by enzymatic or chemical cleavage of the antibody. Other types of covalent modifications of antibodies can be introduced into the molecule by reacting targeted amino acid residues of the antibody with organic derivatizing agents that can react with selected side chains or amino- or carboxy-terminal residues.

[0059] Removal of any carbohydrate moieties present on the antibody can be accomplished chemically or enzymatically. Chemical deglycosylation is described by Hakimuddin et al., 1987, Arch. Biochem. Biophys. 259:52, and Edge et al., 1981, Anal. Biochem., 118:131. Enzymatic cleavage of carbohydrate moieties on the antibody can be accomplished by the use of a variety of endo- and exoglycosidases as described by Thotakura et al., 1987, Meth. Enzymol 138:350. Another type of useful covalent modification involves linking the antibody to one of a variety of nonproteinaceous polymers, such as polyethylene glycol, polypropylene glycol, or polyoxyalkylenes, in the manner set forth in one or more of U.S. Pat. Nos. 4,640,835, 4,496,689, 4,301,144, 4,670,417, 4,791,192, and 4,179,337.

[0060] Epitope Binding In another aspect, the present invention relates to an antibody that recognizes a specific "ANGPT2 antigenic epitope" and "ANGPT2 epitope." In particular, the antibody of the present invention binds to an epitope in the terminal fibrinogen-like domain (FLD) of human ANGTP2 having SEQ ID NO:50. In one aspect, the invention relates to an ANGTP2 antibody or an antigen-binding fragment thereof that binds to at least one amino acid residue within the amino acid region 117 to 148 of the FLD domain of human ANGTP2 as set forth in SEQ ID NO:50 (which is at the C-terminus of the full-length human ANGPT2 protein). In another aspect, the invention relates to an ANGTP2 antibody or antigen-binding fragment thereof that binds to SEQ ID NO:51.

[0061] The sequences of SEQ ID NOs: 50 and 51 are shown in the table below. [Table 3]

[0062] As used herein, the terms "ANGPT2 antigenic epitope" and "ANGPT2 epitope" refer to a molecule (e.g., a peptide) or a fragment of a molecule that can bind to an anti-ANGPT2 antibody or an antigen-binding fragment thereof. These terms further include an ANGPT2 antigenic determinant recognized by any of the antibodies or antibody fragments of the present invention having, for example, a combination of light chain CDRs and heavy chain CDRs selected from the light chain CDRs of SEQ ID NOs: 18-25 and the heavy chain CDRs of SEQ ID NOs: 13-17. In a further embodiment, an ANGPT2 antigenic determinant recognized by any of the antibodies or antibody fragments of the present invention has a combination of light chain CDRs and heavy chain CDRs selected from the light chain CDRs of SEQ ID NOs: 19-25 and the heavy chain CDRs of SEQ ID NOs: 13-17. The ANGPT2 antigen epitope can be contained in a protein, a protein fragment, a peptide, or the like. The epitope is most commonly a protein, a short oligopeptide, an oligopeptide mimetic (i.e., an organic compound that mimics the antibody binding properties of the ANGPT2 antigen), or a combination thereof.

[0063] It has been found that the antibody or antibody fragment of the present invention binds to a unique epitope in the FLD domain of human ANGPT2.Preferably, the anti-ANGPT2 antibody or its antigen-binding fragment binds to at least one amino acid residue in the amino acid region of the FLD domain of human ANGPT2 having SEQ ID NO:50. In one embodiment, the invention provides an anti-ANGPT2 antibody, or antigen-binding fragment thereof, that binds to at least one amino acid residue within the amino acid region of the FLD domain of human ANGPT2 having SEQ ID NO:51. Thus, in the context of epitope binding, the phrase "binds within amino acid region X through Y..." means that an anti-ANGPT2 antibody, or antigen-binding fragment thereof, binds to at least one amino acid residue within the amino acid region specified in the sequence. For example, when an anti-ANGPT2 antibody or an antigen-binding fragment thereof binds to at least one amino acid residue within the amino acid region 117-148, this means that the anti-ANGPT2 antibody or an antigen-binding fragment thereof binds to at least one amino acid residue within the amino acid region 117-148 of the FLD domain of human ANGPT2 having SEQ ID NO:50.

[0064] In another aspect, the anti-ANGPT2 antibody or antigen-binding fragment thereof binds to at least 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 85%, 90%, 95%, or 100% of the amino acid residues within amino acid region 117-148 of the FLD domain of human ANGPT2 having SEQ ID NO:50. Figure 2 shows the results of epitope mapping for chimeric lead CL_209881, ANGPT2-opt-13 (typically an anti-ANGTP2 of the present invention), nevescumab analog (wherein the lysine residue at position 219 is replaced with arginine, K219R), MEDI3617 analog, and LC06. The specific binding site of each molecule to the extracellular FLD domain of human ANGTP2 is highlighted in dark grey. Chimeric leads CL_209881 and ANGPT2-opt-13 (which were derived from CL_209881) bind to epitopes that are different from the epitopes that bind the comparator antibodies. The anti-ANGPT2 antibodies of the invention block the physical interaction between ANGPT2-expressing cells and Tie2-expressing cells, and demonstrate complete inhibition of Tie2 phosphorylation mediated by full-length ANGPT2 oligomers. The anti-ANGPT2 antibody of the present invention is highly selective. At the highest test concentration (500 nM), no binding to human ANGPT1 FLD domain is detected. Furthermore, the anti-ANGPT2 antibody of the present invention does not show non-specific binding to charged or hydrophobic surfaces when tested up to 1 μM.

[0065] Affinity binding data shows that the anti-ANGPT2 antibodies of the present invention have high affinity and are highly selective in blocking ANGPT2. For example, the anti-ANGPT2 antibodies of the present invention have high binding affinity to human, dog, and rabbit ANGPT2 FLD domains. The anti-ANGPT2 antibody of the present invention does not exhibit binding affinity to recombinant mouse ANGPT2 protein and therefore recognizes the mouse ANGPT2 FLD domain (K D Efficacy studies in mice were performed using a mouse cross-reactive tool molecule (nevescu- mab analog) (approximately 200 pM). In studies using disease-relevant and mechanistically relevant preclinical models (db / db UNX mice), treatment with nevescu- mab analog over 8-10 weeks and at dose levels that inhibited free circulating ANGPT2 resulted in a reduction in disease phenotype, including a significant increase in glomerular Tie2 phosphorylation, as well as a significant improvement in kidney structure (reduced glomerular sclerosis and interstitial fibrosis).

[0066] Although the anti-ANGPT2 antibody of the present invention could not be tested in mice for reducing the progression of nephropathy, the weak binding affinity of the anti-ANGPT2 antibody of the present invention to the rat ANGPT2 FLD domain allows the molecule to be tested in an acute mechanistic in vivo permeability model in rats, known as the Miles assay. The Miles assay utilizes VEGF-mediated ANGPT2 release from storage granules in endothelial cells, which leads to rapid vascular destabilization in vivo, and a quantifiable effect. The model was validated using nevescuumab analogs. In the Miles assay in rats, the anti-permeability effect of ANGPT2-opt-13 was observed (49% permeability reduction compared to IgG control), confirming the in vivo activity of the molecule to block ANGPT2-mediated vascular destabilization. The cytotoxicity of the anti-ANGPT2 antibody of the present invention is examined using ANGPT2 cell-based complement-dependent cytotoxicity (CDC) assay.The CDC assay includes two ANGPT1 / ANGPT2 cross-reactive antibodies (MEDI3617 analog and LC06), a comparator antibody that does not cross-react with ANGPT1 (nevescuumab analog), and a representative anti-ANGPT2 antibody of the present invention (ANGPT2-opt-13). The results of the CDC assay (Figures 1A and 1B) show that the ANGPT1 / ANGPT2 cross-reactive comparator antibodies (MEDI3617 analog and LC06), as well as the ANGPT2-specific comparator antibody (nevescuumab analog), all exhibited greater cytotoxicity than ANGPT2-opt-13.

[0067] therapeutic use In one embodiment, the anti-ANGPT2 antibodies or antigen-binding fragments thereof of the present invention are useful for treating or preventing a disease or disorder that can be alleviated by neutralizing ANGPT2 (an "ANGPT2-associated disease or disorder"). In another embodiment, the anti-ANGPT2 antibodies or antigen-binding fragments thereof of the present invention are useful as pharmaceuticals. In one embodiment, the ANGPT2 disease or disorder is selected from the group consisting of ANGPT2-associated disorders, including cardiac hypertrophy, myocardial infarction, ischemia, ischemia-reperfusion injury, hypertensive stroke, pulmonary arterial hypertension, idiopathic pulmonary arterial hypertension, trauma-induced brain damage, asthma, chronic obstructive pulmonary disease (COPD), rheumatoid arthritis, inflammatory bowel disease, multiple sclerosis, preeclampsia and pregnancy-induced hypertension, sepsis, severe sepsis, septic shock, nonalcoholic steatohepatitis (NASH), cirrhosis, minimal change syndrome, focal segmental glomerulosclerosis (FSGS), nephrotic syndrome, diabetic nephropathy (DKD), chronic kidney disease, and the like. These include chronic kidney disease (CKD), diabetic kidney failure, end stage renal disease, ischemia or ischemic reperfusion injury, cancer, hepatocellular carcinoma, idiopathic pulmonary fibrosis (IPF), emphysema, acute lung injury (ALI), acute respiratory distress syndrome (ARDS), severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), vascular hyperpermeability (and related disorders), acute kidney injury, renal cell carcinoma, heart failure, lupus nephritis, Raynaud's, pancreatitis, peripheral arterial disease, congenital heart disease, dengue virus, malaria, hantavirus, edema, regeneration, lupus, interstitial lung disease, scleroderma, retinopathy, diabetic nephropathy, portal hypertension, varicose vein development, and liver transplantation.

[0068] In one embodiment, the present invention relates to methods for treating NASH, cirrhosis, portal hypertension, variceal development variceal bleeding and hepatic encephalopathy. In another embodiment, the present invention relates to a method for treating chronic kidney disease. The term "chronic kidney disease" generally refers to patients with reduced kidney function as measured by glomerular filtration rate (GFR). An estimated GFR (eGFR) of 90 or more (stage 1) is considered normal kidney function; an eGFR of 89-60 (stage 2) is considered mildly reduced kidney function; an eGFR of 59-45 (stage 3a) is considered mild to moderately reduced kidney function; an eGFR of 44-30 (stage 3b) is considered moderate to severely reduced kidney function; and a GFR of 29-15 (stage 4) is considered severely reduced kidney function. In another embodiment, the CKD patient has an albumin to creatine ratio (UACR) of ≥ 30 mg / g and ≥ 20-75 ml / min / 1.73 m2 Or 15~60ml / min / 1.73m 2 have an eGFR of

[0069] In one embodiment, the invention relates to the treatment of stage 2 chronic kidney disease; in another embodiment, the invention relates to the treatment of stage 3a chronic kidney disease; in another embodiment, the invention relates to the treatment of stage 3b chronic kidney disease; and in another embodiment, the invention relates to the treatment of stage 4 chronic kidney disease. In another embodiment, the invention relates to the treatment of chronic kidney disease in patients with ≧eGFR 60; in another embodiment, the invention relates to the treatment of chronic kidney disease in patients with ≧eGFR 45; in another embodiment, the invention relates to the treatment of chronic kidney disease in patients with ≧eGFR 30; and in another embodiment, the invention relates to the treatment of chronic kidney disease in patients with ≧eGFR 20. In another embodiment, the invention relates to the treatment of chronic kidney disease in patients with an eGFR between 20 and 75 ml / min / 1.73 m2. In another embodiment, the present invention relates to a method for treating chronic kidney disease in patients with a UACR≧30 mg / g.

[0070] In another embodiment, the present invention relates to a method for treating chronic kidney disease in rapidly progressing (fast progressor) patients. As used herein, the term rapidly progressing CKD patients have an eGFR of 20-75 ml / min / 1.73 m2 and a decline of > 3 ml / min / 1.73 m2 / year. In another embodiment, rapidly progressing CKD patients have an eGFR of 20-75 ml / min / 1.73 m2 and a decline of > 4 ml / min / 1.73 m2 / year. In another embodiment, the present invention relates to a method for reducing progression to end stage renal disease (ESRD), dialysis and / or cardiovascular events in CKD patents with eGFR between 20-75 ml / min / 1.73 m2. In another embodiment, the present invention relates to a method for reducing the risk of cardiovascular events in CKD patients, for example, patients with an eGFR of 20-75 ml / min / 1.73 m2. In another embodiment, the present invention relates to a method for treating diabetic nephropathy. In another embodiment, the present invention relates to a method for treating vascular hyperpermeability and / or disorders associated therewith, including (i) respiratory disorders associated with vascular hyperpermeability that are not primarily caused by infection (group 1), and (ii) respiratory disorders associated with vascular hyperpermeability that are caused by certain bacterial, viral or fungal parasitic infections (group 2).

[0071] Group 1 non-limiting respiratory disorders associated with vascular hyperpermeability include pulmonary edema, idiopathic interstitial pneumonia, IPF and acute exacerbation of IPF, non-infection-related ARDS and ALI; and Group 2 non-limiting respiratory disorders associated with increased vascular permeability include infection-related ARDS, SARS, MERS, sepsis, severe sepsis and septic shock. Non-infection-related ARDS is understood as ARDS that is not caused by or due to an infection, such as ARDS caused by inhalation of harmful substances (e.g., toxic gases), trauma, pancreatitis, gastric reflux, massive blood transfusion, or burns. Infection-associated ARDS is understood as ARDS caused or contributed to by an infection, such as ARDS caused by sepsis or severe pneumonia. In one embodiment, the present invention relates to the treatment of vascular hyperpermeability.

[0072] In another embodiment, the present invention relates to the treatment of vascular hyperpermeability resulting from or caused by bacterial infections including, but not limited to, Legionella pneumophila, Haemophilus influenzae, Streptococcus pneumonia, Klebsiella, Mycoplasma pneumonia, and Staphylococcus aureus. In another embodiment, the present invention relates to the treatment of vascular hyperpermeability resulting from or caused by fungal infection, including, but not limited to, fungal and parasitic pneumonia. In another embodiment, the present invention relates to the treatment of vascular hyperpermeability resulting from or caused by viral infections, including but not limited to influenza H1N1, respiratory syncytial virus, parainfluenza, adenovirus, and human coronavirus (CoV) infections, such as SARS-CoV, SARS-CoV-2, and MERS-CoV. In another embodiment, the invention relates to the treatment of vascular hyperpermeability associated with ALI, ARDS or SARS.

[0073] Non-therapeutic use The antibodies described herein are useful as affinity purification agents. In this step, the antibodies are immobilized on a solid phase, such as protein A resin, using methods well known in the art. The immobilized antibodies are contacted with a sample containing the ANGPT2 protein (or fragments thereof) to be purified, and the support is then washed with a suitable solvent that substantially removes all materials in the sample other than the ANGPT2 protein that is bound to the immobilized antibody. Finally, the support is washed with another suitable solvent that releases the ANGPT2 protein from the antibody. Anti-ANGPT2 antibodies also are useful in diagnostic assays to detect and / or quantitate ANGPT2 protein, eg, detecting ANGPT2 expression in specific cells, tissues, or serum.

[0074] In some embodiments, it is advantageous to label the antibody with a detectable moiety, for example for diagnostic purposes. A large number of detectable labels are available, including radioisotopes, fluorescent labels, enzyme substrate labels, and the like. The label can be indirectly conjugated to the antibody using various known techniques. For example, the antibody can be conjugated with biotin, any of the three broad categories of labels mentioned above can be conjugated with avidin, or vice versa. Biotin selectively binds to avidin, and the label can be conjugated to the antibody in this indirect manner. Alternatively, to achieve indirect conjugation of the label with the antibody, the antibody can be conjugated with a small hapten (such as digoxin), and one of the different types of labels mentioned above is conjugated with an anti-hapten antibody (e.g., an anti-digoxin antibody). Indirect conjugation of the antibody and the label can be achieved thereby.

[0075] Exemplary radioisotope labels include: 35 S, 14 C. 125 I, 3 H and 131 I. The antibody can be labeled with a radioisotope using the techniques described in, for example, Current Protocols in Immunology, Volumes 1 and 2, 1991, Coligen et al., Ed. Wiley-Interscience, New York, NY, Pubs. Radioactivity can be measured, for example, by scintillation counting. Exemplary fluorescent labels include labels derived from rare earth chelates (europium chelates), or fluorescein and its derivatives, rhodamine and its derivatives, dansyl, Lissamine, phycoerythrin and Texas Red are available. Fluorescent labels can be conjugated to antibodies via known techniques, such as those disclosed in Current Protocols in Immunology, supra, and fluorescence can be quantified, for example, using a fluorometer.

[0076] There are a variety of well-characterized enzyme-substrate labels known in the art (see, for example, U.S. Pat. No. 4,275,149 for a review). In general, the enzyme catalyzes a chemical change in a chromogenic substrate that can be measured using a variety of techniques. For example, the change can be a color change in the substrate that can be measured spectrophotometrically. Alternatively, the enzyme can change the fluorescence or chemiluminescence of the substrate. Techniques for quantifying changes in fluorescence are described above. Chemiluminescent substrates can be electronically excited by a chemical reaction and then emit light that can be measured, for example, using a chemiluminometer, or donate energy to a fluorescent acceptor. Examples of enzyme labels include luciferases, such as firefly luciferase and bacterial luciferase (U.S. Pat. No. 4,737,456), luciferin, 2,3-dihydrophthalazinediones, malate dehydrogenase, urease, peroxidases, such as horseradish peroxidase (HRPO), alkaline phosphatase, β-galactosidase, glucoamylase, lysozyme, saccharide oxidases (such as glucose oxidase, galactose oxidase, and glucose-6-phosphate dehydrogenase), heterocydic oxidases (such as uricase and xanthine oxidase), lactoperoxidase, microperoxidase, and the like. Techniques for conjugating enzymes to antibodies are described, for example, in O'Sullivan et al., 1981, Methods for the Preparation of Enzyme-Antibody Conjugates for use in Enzyme Immunoassay, in Methods in Enzym. (J. Langone & H. Van Vunakis, eds.), Academic press, NY, 73: 147-166.

[0077] Examples of enzyme-substrate combinations include, for example: horseradish peroxidase (HRPO) with hydrogen peroxide as a substrate (hydrogen peroxidase, which oxidizes dye precursors such as orthophenylenediamine (OPD) or 3,3',5,5'-tetramethylbenzidine hydrochloride (TMB)); alkaline phosphatase (AP) with paranitrophenylphosphate as a chromogenic substrate; and β-D-galactosidase (β-D-Gal) with a chromogenic substrate such as p-nitrophenyl-β-D-galactosidase or the fluorogenic substrate 4-methylumbelliferyl-β-D-galactosidase. Many other enzyme-substrate combinations are available to those of skill in the art, for general reviews of which see U.S. Patent Nos. 4,275,149 and 4,318,980. In another embodiment, the anti-ANGPT2 antibody is used unlabeled and is detected using a labeled antibody that binds to the anti-ANGPT2 antibody. The antibodies described herein can be used in any known assay method, such as competitive binding assays, direct and indirect sandwich assays, and immunoprecipitation assays. See, for example, Zola, Monoclonal Antibodies: A Manual of Techniques, pp. 147-158 (CRC Press, Inc. 1987). Diagnostic kits.

[0078] The humanized anti-ANGPT2 antibody can be used in a diagnostic kit, i.e., a packaged combination of a predetermined amount of reagents with instructions for carrying out a diagnostic assay. If the antibody is labeled with an enzyme, the kit can include substrates and cofactors required by the enzyme, such as substrate precursors that provide detectable chromophores or fluorophores. In addition, other additives can be included, such as stabilizers, buffers (e.g., blocking buffers or lysis buffers). The relative amounts of the various reagents can vary widely to provide concentrations in solution of the reagents that substantially optimize the sensitivity of the assay. The reagents can be provided as dry powders, usually lyophilized, that contain excipients that, when dissolved, result in a reagent solution with the appropriate concentration.

[0079] Diagnostic Kits Anti-ANGPT2 antibodies can be used in diagnostic kits, i.e., packaged combinations of reagents in predetermined amounts, including instructions for carrying out diagnostic assays. When the antibody is labeled with an enzyme, the kit can include substrates and cofactors required by the enzyme, such as substrate precursors that provide detectable chromophores or fluorophores. In addition, other additives can be included, such as stabilizers, buffers (e.g., blocking buffers or lysis buffers). The relative amounts of various reagents can vary widely to provide concentrations in solution of the reagents that substantially optimize the sensitivity of the assay. Reagents can be provided as dry powders, usually lyophilized, that include excipients that, when dissolved, result in a reagent solution with the appropriate concentration.

[0080] Compositions and their administration The composition comprising the anti-ANGPT2 antibody or antigen-binding fragment thereof can be administered to a subject at risk of ANGPT2-related disease or disorder described herein. The present invention further provides the use of the anti-ANGPT2 antibody or antigen-binding fragment thereof in the manufacture of a medicament for the prevention or treatment of ANGPT2 disease. As used herein, the term "subject" refers to any mammalian patient to which the anti-ANGPT2 antibody or antigen-binding fragment thereof can be administered, including, for example, humans and certain non-human mammals such as primates and dogs. Subjects specifically intended for treatment using the methods described herein include humans. The anti-ANGPT2 antibody or antigen-binding fragment thereof can be administered either alone or in combination with other compositions. Preferred antibodies for use in such pharmaceutical compositions include those comprising an antibody according to the present invention.

[0081] A variety of delivery systems are known and can be used to administer anti-ANGPT2 antibody or antigen-binding fragment thereof. Methods of introduction include, but are not limited to, intravitreal, eye drops, intradermal, intramuscular, intraperitoneal, intravenous, subcutaneous, intranasal, epidural and oral routes. Anti-ANGPT2 antibody or antigen-binding fragment thereof can be administered, for example, by infusion, bolus or injection, and can be administered together with other biologically active agents. Administration can be systemic or local. In a preferred embodiment, administration is by intravitreal injection. Formulations for such injection can be prepared, for example, in pre-filled syringes. The anti-ANGPT2 antibody or antigen-binding fragment thereof can be administered as a pharmaceutical composition comprising a therapeutically effective amount of the anti-ANGPT2 antibody or antigen-binding fragment thereof and one or more pharma- ceutically compatible ingredients. In a typical embodiment, the pharmaceutical composition is formulated according to routine procedures as a pharmaceutical composition adapted for intravenous or subcutaneous administration to humans. Typically, compositions for administration by injection are solutions in sterile isotonic aqueous buffer. If necessary, the pharmaceutical product may also include a solubilizing agent and a local anesthetic, such as lignocaine, to ease pain at the injection site. Generally, the ingredients are supplied separately or mixed together in unit dosage form, for example as a dry lyophilized powder or water-free concentrate, in a hermetically sealed container, such as an ampoule or sachette, indicating the amount of active agent. When the pharmaceutical product is administered by injection, it may be dispensed into an injection bottle containing sterile pharmaceutical grade water or saline. When the pharmaceutical product is administered by injection, an ampoule of sterile water for injection or saline may be provided so that the ingredients can be mixed prior to administration.

[0082] Further, the pharmaceutical composition may be provided as a pharmaceutical kit comprising (a) a container containing the anti-ANGPT2 antibody or antigen-binding fragment thereof in lyophilized form, and (b) a second container containing a pharma- ceutically acceptable diluent for injection (e.g., sterile water). The pharma-ceutically acceptable diluent may be used for reconstitution or dilution of the lyophilized anti-ANGPT2 antibody or antigen-binding fragment thereof. Optionally, associated with such container may be a label in a form prescribed by a governmental agency regulating the manufacture, use, and sale of pharmaceuticals or biological products, which label reflects approval by the agency of manufacture, use, or sale for human administration. The amount of anti-ANGPT2 antibody or its antigen-binding fragment that is effective for treating or preventing ANGPT2-related disease or disorder can be determined by standard clinical techniques.In addition, in vitro assays can be used to help identify optimal dosage ranges.The exact dose that can be used in the formulation also depends on the route of administration and the stage of the disorder, and should be determined by the judgment of the practitioner and the circumstances of each patient.Effective amounts can be estimated from dose-response curves derived from in vitro or animal model test systems.

[0083] For example, the toxicity and therapeutic efficacy of an anti-ANGPT2 antibody or antigen-binding fragment thereof may be determined by the ED 50 (the dose that is therapeutically effective in 50% of the population) can be determined in cell cultures or experimental animals by standard pharmaceutical procedures. Anti-ANGPT2 antibodies or antigen-binding fragments thereof that exhibit larger therapeutic indices are preferred. The data obtained from cell culture assays and animal studies can be used to define a range of dosages for use in humans. The dosage of the anti-ANGPT2 antibody or antigen-binding fragment thereof can be adjusted to an ED that results in little or no toxicity. 50 The circulating concentration of the ANGPT2 antibody or antigen-binding fragment thereof is typically within a range including the IC50-IC10 determined in cell culture. Dosages may vary within this range depending on the dosage form used and the route of administration utilized. For any anti-ANGPT2 antibody or antigen-binding fragment thereof used in the method, the therapeutically effective dose can be estimated initially from cell culture assays. Dosages are determined based on the IC50-IC10 determined in cell culture. 50 The compound can be formulated in animal models to achieve a circulating plasma concentration range that includes the concentration of the test compound that achieves a half-maximal inhibition of symptoms (i.e., the concentration of the test compound that achieves a half-maximal inhibition of symptoms). Such information can be used to more accurately determine useful doses in humans. Levels in plasma can be measured, for example, by high performance liquid chromatography, ELISA, etc.

[0084] For intravitreal injection of ANGPT2 antibodies, longer intervals between treatments are generally preferred. Due to improved potency, the ANGPT2 antibodies of the present invention can be administered at longer intervals. In one embodiment, the ANGPT2 antibody is administered every 6 weeks, or every 7 weeks, or every 8 weeks, or every 9 weeks, or every 10 weeks, or every 11 weeks, or every 12 weeks. In a further embodiment, the ANGPT2 antibody of the present invention is administered once every 3 months. The antibodies of the invention may be formulated in dosages including, but not limited to, 20 mg / ml to 180 mg / ml; or 20 mg / ml, 30 mg / ml, 40 mg / ml, 50 mg / ml, 60 mg / ml, 70 mg / ml, 80 mg / ml, 90 mg / ml or 100 mg / ml. Preferably, the antibodies of the invention may be formulated in a liquid formulation of about 50 mg / ml to about 150 mg / ml. In some embodiments, a pharmaceutical composition comprising an anti-ANGPT2 antibody or antigen-binding fragment thereof may further comprise a therapeutic agent, either conjugated or unconjugated to a binding agent.

[0085] Such combined therapeutic administration may have additive or synergistic effects on disease parameters (eg, severity of symptoms, number of symptoms, or frequency of recurrences). With respect to the treatment regimen for combinatorial administration, in a specific embodiment, the anti-ANGPT2 antibody or antigen-binding fragment thereof is administered simultaneously with the therapeutic agent. In another specific embodiment, the therapeutic agent is administered at least one hour to several months prior to or following administration of the anti-ANGPT2 antibody or antigen-binding fragment thereof, for example, at least one hour, five hours, twelve hours, one day, one week, one month, or three months prior to or following administration of the anti-ANGPT2 antibody or antigen-binding fragment thereof. The compounds of the invention can be used alone or in combination with one or more additional therapeutic agents. Non-limiting examples of additional therapeutic agents include: Antidiabetic agents, such as alpha-glucosidase inhibitors (e.g., miglitol and acarbose), amylin analogs (e.g., pramlintide), dipeptidyl peptidase 4 inhibitors (e.g., alogliptin, sitagliptin, saxagliptin, and linagliptin), incretin mimetics (e.g., liraglutide, exenatide, liraglutide, exenatide, dulaglutide, albiglutide, and lixisenatide), incretin mimetics (e.g., liraglutide, exenatide, dulaglutide, albiglutide, and lixisenatide), insulin, meglitinides (e.g., repaglinide and nateglinide), biguanides (e.g., metformin); SGLT-2 inhibitors (e.g., canagliflozin, empagliflozin, and dapagliflozin), sulfonylureas (e.g., chlorpropamide, glimepiride, glyburide, glipizide, glyburide, tolazamide, and tolbutamide), and thiazolidinediones (e.g., rosiglitazone and pioglitazone);

[0086] Angiotensin II receptor antagonists (angiotensin receptor blockers (ARBs)), such as candesartan, eprosartan, candesartan, irbesartan, losartan, olmesartan, telmisartan, valsartan, azilsartan and medoxomil; Angiotensin-converting enzyme inhibitors (e.g., benazepril, captopril, enalapril, fosinopril, lisinopril, moexipril, and perindopril); Anticoagulants (e.g., dabigatran, actylise, warfarin, heparin and acetylsalicylic acid); Bronchodilators, including short- and long-action beta agonists (e.g., albuterol, levalbuterol, salmeterol, formoterol, arformoterol, vilanterol, indacaterol, and olodaterol) and short- and long-acting anticholinergics (ipratropium, tiotropium, umeclidinium, glycopyrrolatei, and aclidinium);

[0087] Steroids, such as fluticasone and budesonide; Antimalarials, such as hydroxychloroquine or chloroquine; Virostatic nucleosid analogues, such as remdesivir; and HIV-protease inhibitors, such as lopinavir-ritonavir. Polynucleotides, Vectors, Host Cells and Recombinant Methods The present invention relates to isolated polynucleotides comprising sequences encoding anti-ANGPT2 antibodies, vectors and host cells comprising the polynucleotides, and recombinant techniques for producing antibodies. The isolated polynucleotides can encode any desired form of anti-ANGPT2 antibodies, including, for example, full-length monoclonal antibodies, Fab, Fab', F(ab')2 and Fv fragments, bispecific antibodies, linear antibodies, single-chain antibody molecules, and multispecific antibodies formed from antibody fragments.

[0088] The polynucleotide comprising the sequence encoding anti-ANGPT2 antibody or its fragment or chain may be fused to one or more control or regulatory sequences known in the art and may be contained in a suitable expression vector or host cell known in the art.Each polynucleotide molecule encoding heavy or light chain variable domain may be fused independently to a polynucleotide sequence encoding a constant domain, such as a human constant domain, to allow the production of intact antibody.Alternatively, polynucleotides or parts thereof may be fused together to provide a template for the production of single chain antibody. For recombinant production, a polynucleotide encoding the antibody is inserted into a replicable vector for cloning (amplification of the DNA) or for expression. Many suitable vectors for expressing recombinant antibodies are available. Vector components generally include, but are not limited to, one or more of the following: a signal sequence, an origin of replication, one or more marker genes, an enhancer element, a promoter, and a transcription termination sequence.

[0089] Anti-ANGPT2 antibodies can also be produced as fusion polypeptides, in which the antibody is fused to a heterologous polypeptide, such as a signal sequence or other polypeptide that has a specific cleavage site at the amino terminus of the mature protein or polypeptide. The heterologous signal sequence selected is typically one that is recognized and processed (i.e., cleaved by a signal peptidase) by the host cell. For prokaryotic host cells that do not recognize and process the anti-ANGPT2 antibody signal sequence, the signal sequence can be replaced by a prokaryotic signal sequence. The signal sequence can be, for example, alkaline phosphatase, penicillinase, lipoprotein, heat-stable enterotoxin II leader, etc. For yeast secretion, the native signal sequence can be replaced with a leader sequence obtained from, for example, yeast invertase alpha factor (including Saccharomyces and Kluyveromyces alpha factor leaders), acid phosphatase, C. albicans glucoamylase, or the signal described in WO90 / 13646. In mammalian cells, mammalian signal sequences as well as viral secretory leaders, such as the herpes simplex gD signal, can be used. DNA for such precursor regions is ligated in frame to DNA encoding the humanized anti-ANGPT2 antibody.

[0090] Expression and cloning vectors contain a nucleic acid sequence that allows the vector to replicate in one or more selected host cells. In general, in cloning vectors, this sequence allows the vector to replicate independently of the host chromosomal DNA and includes an origin of replication or an autonomously replicating sequence. Such sequences are well known for a variety of bacteria, yeasts and viruses. The origin of replication from the plasmid pBR322 is suitable for most Gram-negative bacteria, the 2-v plasmid origin is suitable for yeast, and various viral origins (SV40, polyoma, adenovirus, VSV and BPV) are useful for cloning vectors in mammalian cells. Generally, the origin of replication component is not needed for mammalian expression vectors (the SV40 origin may typically be used only because it contains the early promoter). Expression and cloning vectors may contain genes encoding selectable markers that facilitate identification of expression. Typical selectable marker genes confer resistance to antibiotics or other toxins, such as ampicillin, neomycin, methotrexate or tetracycline, or alternatively encode proteins that complement auxotrophic deficiencies or provide specific nutrients that are otherwise absent in complex media, such as the gene encoding D-alanine racemase for Bacillus. One example of a selection scheme utilizes drugs to stop the growth of host cells. Those cells that are successfully transformed with a heterologous gene produce a protein that confers drug resistance, and thus survive the selection regimen. Examples of such dominant selection use the drugs neomycin, mycophenolic acid, and hygromycin. Common selectable markers for mammalian cells are those that allow the identification of cells that are capable of incorporating nucleic acid encoding a humanized anti-ANGPT2 antibody, such as DHFR (dihydrofolate reductase), thymidine kinase, metallothionein-I and -II (such as primate metallothionein genes), adenosine deaminase, ornithine decarboxylase, and the like. Cells transformed with a DHFR selection gene are first identified by culturing all transformants in a culture medium containing methotrexate (Mtx), a competitive antagonist of DHFR. When wild-type DHFR is used, a suitable host cell is a Chinese hamster ovary (CHO) cell line that is deficient in DHFR activity (e.g., DG44).

[0091] Alternatively, host cells transformed or co-transformed with a DNA sequence encoding an anti-ANGPT2 antibody, a wild-type DHFR protein, and another selectable marker, such as aminoglycoside 3'-phosphotransferase (APH), (particularly wild-type hosts containing endogenous DHFR), can be selected by growing the cells in medium containing a selection agent for the selectable marker, such as an aminoglycoside antibacterial drug, e.g., kanamycin, neomycin, or G418. See, e.g., U.S. Patent No. 4,965,199. When recombinant production is carried out in yeast cells as host cells, the TRP1 gene present in the yeast plasmid YRp7 (Stinchcomb et al., 1979, Nature 282: 39) can be used as a selectable marker. The TRP1 gene provides a selection marker for mutant strains of yeast lacking the ability to grow in tryptophan, such as ATCC No. 44076 or PEP4-1 (Jones, 1977, Genetics 85:12). The presence of the trp1 lesion in the yeast host cell genome then provides an effective environment for detecting transformation by growth in the absence of tryptophan. Similarly, Leu2p-deficient yeast strains, such as ATCC 20,622 and 38,626, are complemented by known plasmids carrying the LEU2 gene.

[0092] In addition, vectors derived from the 1.6 μm circular plasmid pKD1 can be used for transformation of Kluyveromyces yeast. Alternatively, an expression system for large-scale production of recombinant calf chymosin was reported for K. lactis (Van den Berg, 1990, Bio / Technology 8:135). A stable multicopy expression vector for secretion of mature recombinant human serum albumin by industrial strains of Kluyveromyces has also been disclosed (Fleer et al., 1991, Bio / Technology 9:968-975). Expression and cloning vectors usually contain a promoter that is recognized by the host organism and is operably linked to the nucleic acid molecule encoding the anti-ANGPT2 antibody or its polypeptide chain. Suitable promoters for use with prokaryotic hosts include the phoA promoter, β-lactamase and lactose promoter systems, alkaline phosphatase, tryptophan (trp) promoter system and hybrid promoters such as the tac promoter. Other known bacterial promoters are also suitable. Promoters for use in bacterial systems also contain a Shine-Dalgamo (SD) sequence operably linked to the DNA encoding the humanized anti-ANGPT2 antibody.

[0093] Many eukaryotic promoter sequences are known. Virtually all eukaryotic genes have an AT-rich region located approximately 25-30 bases upstream from the site where transcription is initiated. Another sequence found 70-80 bases upstream from the start of transcription of many genes is a CNCAAT region, where N can be any nucleotide. At the 3' end of most eukaryotic genes is an AATAAA sequence, which may be a signal for addition of a poly A tail to the 3' end of the coding sequence. All of these sequences are suitable for insertion into eukaryotic expression vectors. Examples of suitable promoting sequences for use with yeast hosts include promoters for 3-phosphoglycerate kinase or other glycolytic enzymes, such as enolase, glyceraldehyde-3-phosphate dehydrogenase, hexokinase, pyruvate decarboxylase, phosphofructokinase, glucose-6-phosphate isomerase, 3-phosphoglycerate mutase, pyruvate kinase, triosephosphate isomerase, phosphoglucose isomerase, and glucokinase.

[0094] Inducible promoters have the added advantage of transcription being regulated by growth conditions. These include yeast promoter regions for alcohol dehydrogenase 2, isocytochrome C, acid phosphatase, enzyme derivatives involved in nitrogen metabolism, metallothionein, glyceraldehyde-3-phosphate dehydrogenase, and enzymes involved in maltose and galactose utilization. Suitable vectors and promoters for use in yeast expression are further described in EP 73,657. Yeast enhancers are also advantageously used with yeast promoters. Anti-ANGPT2 antibody transcription from a vector in a mammalian host cell is regulated by a promoter derived from the genome of a virus, such as polyoma virus, fowlpox virus, adenovirus (such as adenovirus 2), bovine papilloma virus, avian sarcoma virus, cytomegalovirus, retrovirus, hepatitis B virus, and simian virus 40 (SV40), from a heterologous mammalian promoter, e.g., an actin promoter or an immunoglobulin promoter, or from a heat shock promoter, provided that such promoter is compatible with the host cell system.

[0095] The early and late promoters of the SV40 virus are conveniently obtained as an SV40 restriction fragment, which also contains the SV40 viral origin of replication. The immediate early promoter of the human cytomegalovirus is conveniently obtained as a HindIII E restriction fragment. A system for expressing DNA in a mammalian host using bovine papilloma virus as a vector is disclosed in U.S. Pat. No. 4,419,446. A modification of this system is described in U.S. Pat. No. 4,601,978. See also Reyes et al., 1982, Nature 297:598-601, which discloses the expression of human p-interferon cDNA in mouse cells under the control of the thymidine kinase promoter from herpes simplex virus. Alternatively, the Rous sarcoma virus long terminal repeat can also be used as a promoter. Another useful element that can be used in a recombinant expression vector is an enhancer sequence, which is used to increase the transcription of DNA encoding an anti-ANGPT2 antibody by higher eukaryotes. A large number of enhancer sequences are now known from mammalian genes (e.g., globin, elastase, albumin, alpha-fetoprotein, and insulin). Typically, however, enhancers from eukaryotic cell viruses are used. Examples include the SV40 enhancer behind the replication origin (bp 100-270), the cytomegalovirus early promoter enhancer, the polyoma enhancer behind the replication origin, and adenovirus enhancers. See also Yaniv, 1982, Nature 297:17-18 for a description of enhancing elements for the activation of eukaryotic promoters. The enhancer can be spliced ​​into the vector at the 5' or 3' position of the anti-ANGPT2 antibody coding sequence, but is preferably located at a site 5' from the promoter.

[0096] Expression vectors used in eukaryotic host cells (nucleated cells from yeast, fungi, insects, plants, animals, humans or other multicellular organisms) can also contain sequences required for termination of transcription and stabilization of mRNA. Such sequences are generally available from the 5' and sometimes 3' untranslated regions of eukaryotic or viral DNA or cDNA. These regions contain nucleotide segments that are transcribed as polyadenylated fragments in the untranslated portion of the mRNA that codes for anti-ANGPT2 antibody. One useful transcription termination component is the bovine growth hormone polyadenylation region. See WO94 / 11026 and the expression vectors disclosed therein. In some embodiments, anti-ANGPT2 antibody can be expressed using the CHEF system (see, for example, U.S. Patent No. 5,888,809, the disclosure of which is incorporated herein by reference).

[0097] Suitable host cells for cloning or expressing DNA in the vectors herein are prokaryotes, yeast or higher eukaryotic cells as described above. Suitable prokaryotes for this purpose include eubacteria such as gram-negative or gram-positive bacteria, such as Escherichia coli, such as E. coli, Enterobacter, Erwinia, Klebsilea, Proteus, Salmonella, such as Salmonella typhimurium, Serratia, such as Serratia marcescans and Shigella, and bacilli, such as B. subtilis and B. licheniformis (e.g. B. licheniformis 41P, disclosed in DD 266,710 published April 12, 1989), Pseudomonas, such as P. aeruginosa and Streptomyces. One preferred E. coli cloning host is E. coli 294 (ATCC 31,446), although other strains such as E. coli B, E. coli X1776 (ATCC 31,537), and E. coli W3110 (ATCC 27,325) are also suitable. These examples are illustrative rather than limiting.

[0098] In addition to prokaryotes, eukaryotic microbes such as filamentous fungi or yeast are suitable cloning or expression hosts for anti-ANGPT2 antibody-encoding vectors. Saccharomyces cerevisiae, or common baker's yeast, is the most commonly used among lower eukaryotic host microorganisms. However, Schizosaccharomyces pombe; Kluyveromyces hosts such as K. lactis, K. fragilis (ATCC 12,424), K. bulgaricus (ATCC 16,045), K. wickeramii (ATCC 24,178), K. waltii (ATCC 56,500), K. drosophilarum (ATCC 36,906), K. thermotolerans and K. marxianus; Yarrowia (EP 402,226); Pichia partoris; pastors (EP 183,070); Candida; Trichoderma reesia (EP 244,234); Neurospora crassa; Schwanniomyces, e.g., Schwanniomyces occidentalis; and numerous other genera, species and strains of filamentous fungi, e.g., Neurospora, Penicillium, Tolypocladium, and Aspergillus hosts, e.g., A. nidulans and A. niger, are commonly available and useful herein.

[0099] Suitable host cells for the expression of glycosylated anti-ANGPT2 antibodies are derived from multicellular organisms. Examples of invertebrate cells include plants and insect cells, including many baculovirus strains and variants and corresponding permissive insect host cells derived from hosts such as Spodoptera frugiperda (caterpillar), Aedes aegypti (mosquito), Aedes albopictus (mosquito), Drosophila melanogaster (fruit fly) and Bombyx mori (silkworm). Various virus strains for transfection are publicly available; for example, the L-1 variant of Autographa californica NPV and the Bm-5 strain of Bombyx mori NPV, such viruses can be used specifically for transfection of Spodoptera frugiperda cells.

[0100] Plant cell cultures of cotton, corn, potato, soybean, petunia, tomato, and tobacco may also be utilized as hosts. The anti-ANGPT2 antibody or antigen-binding fragment thereof of the present invention may be incorporated into a viral vector, i.e., a polynucleotide encoding the anti-ANGPT2 antibody or antigen-binding fragment thereof is introduced into a viral vector and then expressed in the patient's body after infection with the virus.

[0101] In another embodiment, expression of anti-ANGPT2 is carried out in vertebrate cells. Propagation of vertebrate cells in culture (tissue culture) has become a routine procedure and techniques are widely available. Examples of useful mammalian host cell lines are SV40 transformed monkey kidney CV1 line (COS-7, ATCC CRL1651), human embryonic kidney line (293 or 293 cells subcloned for growth in suspension culture, (Graham et al., 1977, J. Gen Virol. 36: 59), baby hamster kidney cells (BHK, ATCC CCL10), Chinese hamster ovary cells / -DHFR1 (CHO, Urlaub et al., 1980, Proc. Natl. Acad. Sci. USA 77: 4216; e.g., DG44), mouse Sertoli cells (TM4, Mather, 1980, Biol. Reprod. 23:243-251), monkey kidney cells (CV1 ATCC CCL70), African green monkey kidney cells (VERO-76, ATCC CRL-1587), human cervical carcinoma cells (HELA, ATCC CCL2), canine kidney cells (MDCK, ATCC CCL34), buffalo rat hepatocytes (BRL 3A, ATCC CRL1442), human lung cells (W138, ATCC CCL75), human hepatocytes (Hep G2, HB8065), mouse mammary tumor (MMT060562, ATCC CCL51), TR1 cells (Mather et al., 1982, Annals NY Acad. Sci. 383: 44-68), MRC5 cells, FS4 cells and a human hepatoma line (HepG2). Host cells are transformed with the expression or cloning vectors described above for producing anti-ANGPT2 antibodies and cultured in conventional nutrient media modified as appropriate for inducing promoters, selecting transformants, or amplifying genes encoding the desired sequences.

[0102] The host cells used to produce the anti-ANGPT2 antibodies described herein can be cultured in a variety of media. Commercially available media such as Ham's F10 (Sigma-Aldrich Co., St. Louis, Mo.), Minimal Estimation Medium (MEM), (Sigma-Aldrich Co.), RPMI-1640 (Sigma-Aldrich Co.) and Dulbecco's Modified Eagle's Medium (DMEM), Sigma-Aldrich Co. are suitable for culturing the host cells. In addition, the media described in Ham et al., 1979, Meth. Enz. 58: 44; Barnes et al., 1980, Anal. Biochem. 102: Any medium described in one or more of US Patent Nos. 255, 4,767,704, 4,657,866, 4,927,762, 4,560,655, 5,122,469, WO90 / 103430 and WO87 / 00195 may be used as a culture medium for host cells. Any of these media may be supplemented with hormones and / or other growth factors (such as insulin, transferrin or epidermal growth factor), salts (such as sodium chloride, calcium, magnesium and phosphate), buffers (such as HEPES), nucleotides (such as adenosine and thymidine), antibiotics (such as gentamicin), trace elements (defined as inorganic compounds usually present at final concentrations in the micromolar range) and glucose or an equivalent energy source, as required. Other supplements may also be included at appropriate concentrations known to those skilled in the art. Culture conditions such as temperature, pH, etc. are those previously used with the host cell selected for expression and will be apparent to those skilled in the art.

[0103] When using recombinant techniques, antibodies may be produced intracellularly, in the periplasmic space, or directly secreted into the medium. If the antibody is produced intracellularly, as a first step the cells may be disrupted to release the protein. Particulate debris, host cells or lysed fragments may be removed, for example, by centrifugation or ultrafiltration. Carter et al., 1992, Bio / Technology 10:163-167, describes a procedure for isolating antibodies secreted into the periplasmic space of E. coli. Briefly, cell paste is thawed in the presence of sodium acetate (pH 3.5), EDTA and phenylmethylsulfonyl fluoride (PMSF) for about 30 minutes. Cell debris may be removed by centrifugation. If the antibody is secreted into the medium, the supernatant from such expression systems is generally first concentrated using a commercially available protein concentration filter, for example, an Amicon or Millipore Pellicon ultrafiltration device. Protease inhibitors such as PMSF may be included in any of the foregoing steps to inhibit proteolysis, and antibiotics may be included to prevent the growth of adventitious contaminants.Various methods may be used to isolate antibodies from host cells.

[0104] The antibody composition prepared from the cells can be purified using, for example, hydroxylapatite chromatography, gel electrophoresis, dialysis and affinity chromatography, with affinity chromatography being a typical purification technique. The suitability of protein A as an affinity ligand depends on the species and isotype of any immunoglobulin Fc domain present in the antibody. Protein A can be used to purify antibodies based on human gamma 1, gamma 2 or gamma 4 heavy chains (see, for example, Lindmark et al., 1983 J. Immunol. Meth. 62:1-13). Protein G is recommended for all mouse isotypes and for human gamma 3 (see, for example, Guss et al., 1986 EMBO J. 5:1567-1575). The matrix to which the affinity ligand is attached is most often agarose, but other matrices are available. Mechanically stable matrices such as controlled pore glass or poly(styrenedivinyl)benzene allow for faster flow rates and shorter processing times than can be achieved with agarose. H3 If the antibody contains a nucleotide sequence similar to that of the nucleotide sequence ...

[0105] Following any preliminary purification steps, the mixture containing the antibody of interest and contaminants may be subjected to low pH hydrophobic interaction chromatography, typically performed at a low salt concentration (e.g., about 0-0.25 M salt) using an elution buffer at a pH between about 2.5-4.5. Also included are nucleic acids that hybridize under low, medium and high stringency conditions defined herein to all or part of the nucleotide sequence represented by the isolated polynucleotide sequence encoding ANGPT2-antibody or antibody fragment (e.g., the part encoding variable region).The hybridizing portion of the hybridizing nucleic acid is typically at least 15 (e.g., 20, 25, 30 or 50) nucleotides in length.The hybridizing portion of the hybridizing nucleic acid is at least 80%, for example, at least 90%, at least 95% or at least 98% identical to the part or all sequence of the nucleic acid encoding anti-ANGPT2 polypeptide (e.g., heavy or light chain variable region) or its complement.The hybridizing nucleic acid of the type described herein can be used, for example, as a cloning probe, primer, for example, PCR primer or diagnostic probe.

[0106] In one embodiment, the present invention provides An isolated polynucleotide or polynucleotides comprising a heavy chain as set forth in SEQ ID NO:31 or a sequence encoding a heavy chain variable region as set forth in SEQ ID NO:3; and a light chain as set forth in SEQ ID NO:32 or a sequence encoding a light chain variable region as set forth in SEQ ID NO:8. or An isolated polynucleotide or polynucleotides comprising a sequence encoding a heavy chain as set forth in SEQ ID NO:33 or a heavy chain variable region as set forth in SEQ ID NO:4; and a sequence encoding a light chain as set forth in SEQ ID NO:34 or a light chain variable region as set forth in SEQ ID NO:9. or An isolated polynucleotide or polynucleotides comprising a heavy chain as set forth in SEQ ID NO:35 or a sequence encoding a heavy chain variable region as set forth in SEQ ID NO:5; and a light chain as set forth in SEQ ID NO:36 or a sequence encoding a light chain variable region as set forth in SEQ ID NO:10. or An isolated polynucleotide or polynucleotides comprising a sequence encoding a heavy chain as set forth in SEQ ID NO:37 or a heavy chain variable region as set forth in SEQ ID NO:6; and a sequence encoding a light chain as set forth in SEQ ID NO:38 or a light chain variable region as set forth in SEQ ID NO:11. or An isolated polynucleotide or polynucleotides comprising a heavy chain as set forth in SEQ ID NO:39 or a sequence encoding a heavy chain variable region as set forth in SEQ ID NO:7; and a light chain as set forth in SEQ ID NO:40 or a sequence encoding a light chain variable region as set forth in SEQ ID NO:12. Regarding.

[0107] In the above-mentioned anti-ANGPT2 antibodies and antibody fragments, it is understood that the nucleic acid sequences encoding the CDRs remain unchanged (unchanged with respect to the amino acids they encode, equivalent DNA sequences are possible due to codon degeneracy), but the surrounding regions, e.g., the FR regions, may be manipulated.

[0108] manufactured goods In another embodiment, an article of manufacture is included that contains materials useful for the treatment of the disorders described above. The article of manufacture includes a container and a label. Suitable containers include, for example, bottles, vials, syringes, and test tubes. The containers can be formed from a variety of materials, such as glass or plastic. The container holds a composition that is effective for treating a condition and may have a sterile access port. For example, the container may be an intravenous solution bag or a vial with a stopper that can be pierced by a hypodermic needle. The active agent in the composition is an anti-ANGPT2 antibody or an antigen-binding fragment thereof. The label on or associated with the container indicates that the composition is used for the treatment of a selected condition. The article of manufacture may further include a second container that contains a pharma- ceutically acceptable buffer, such as phosphate buffered saline, Ringer's solution, and dextrose solution. It may further include other materials desirable from a commercial and user standpoint, including other buffers, diluents, filters, needles, syringes, and package inserts containing instructions for use. The present invention is further described in the following examples, which are not intended to limit the scope of the invention. EXAMPLES

[0109] Antibodies ANGPT2-opt-1, ANGPT2-opt-2, ANGPT2-opt-13, ANGPT2-opt-19 and ANGPT2-opt-31 are characterized along with comparator antibodies nesvacumab analog, MEDI3617 analog and LC06. These comparator antibodies were generated using standard procedures based on the published sequences described below.

[0110] [Table 4] Data for these antibodies is provided below.

[0111] Example 1 Antibody generation (immunization) Wild-type CD1 mice are immunized with recombinant human and mouse ANGPT2 DNA and human and mouse ANGPT2 protein. Freund's complete adjuvant, Freund's incomplete adjuvant, Titermax or Gerbu are used as adjuvants at various points to enhance antibody response. Serology is then evaluated by ELISA. Selected serologically positive mice are given a final boost before B cell isolation. All selected mice show positive antibody titers in serum. At the end of the immunization regimen, splenocytes are harvested for recovery of antigen-specific B cells.

[0112] Example 2 Production of humanized antibodies The chimeric lead CL-209881_VL was selected for further optimization. The chimeric lead has a variable light chain corresponding to SEQ ID NO:2 and a variable heavy chain corresponding to SEQ ID NO:1. Thirty-three sequences (leads) were selected and optimized. Subsequent studies on selection of six antibodies for further scale-up. Example 3 Sequence trends in CDRs The sequences of the CDRs were examined for the presence of any potential propensities such as N-glycosylation sites, strong deamidation motifs (NG, NS, NH, NA, ND, NT, NN), aspartic acid isomerization motifs (DG), fragmentation motifs (DG, DS), cysteines, etc. These amino acids or motifs may undergo chemical reactions and impart undesirable heterogeneity to the product, and may also have a negative impact on target binding and function. For these reasons, it is preferable to remove such amino acids or motifs (if any) from the CDRs.

[0113] Example 4 immunogenicity The immunogenicity of the sequence is evaluated computationally using an algorithm provided through licensing company EpiVax, Inc., Providence, Rhode Island. The EpiMatrix Treg-adj score takes into account T cell epitopes and Treg epitopes. The lower the immunogenicity score, the more likely the sequence is not immunogenic. In general, negative scores are considered a low risk of immunogenicity, while positive scores are considered an indication of potential immunogenicity.

[0114] Example 5 Epitope Information material Water (Sigma Aldrich, P / N 37877-4L) Acetonitrile (Sigma Aldrich, P / N 34998-4L) Formic Acid (Fluka, P / N 94318) Urea (Sigma Aldrich, P / N 51456-500G) TCEP-HCl-10g (Thermo Scientific-Pierce, P / N 20491) Disodium phosphate (Sigma Aldrich, P / N S7907-100G) Monosodium phosphate (Sigma Aldrich, P / N S8282-500G) ACQUITY UPLC BEH C18 VanGuard Precolumn, 130Å, 1.7μm, 2.1mm X 5mm (Waters Technologies Corp, 186003975) Poroszyme® Immobilized Pepsin Cartridge, 2.1mm x 30mm (Life Technologies Corp, 2313100) Acquity UPLC BEH C18 column 1.7um, 1mm x 50mm (Waters, 186002344)

[0115] Solvent A: 0.1% formic acid / 99% water / 1% acetonitrile Solvent B: 0.1% formic acid / 5% water / 95% acetonitrile Water buffer: H2O 10 mM sodium phosphate pH 7.4 Deuterium buffer: D2O 10mM sodium phosphate pH 7.4 Quench buffer: water 8M urea, 0.4M TCEP-HCl For epitope mapping control samples, the antigen is run with or without the antibody. To determine the table of antigen peptides, the protocol is first run using water buffer instead of deuterium buffer. 4 μL of sample is mixed with 40 μL of deuterium buffer. This mixture is incubated at 20 °C for multiple time points (1, 2 and 4 min). 40 μL of the mixture is then transferred to 40 μL of 4 °C, quench buffer (4 M urea, 0.4 M Tcep-HCl) and mixed. 60 μL of quenched protein is injected for digestion on a pepsin column by running 200 μL / mL of solvent A: 0.1% formic acid / 99% water / 1% acetonitrile for 2 min. Peptides are subsequently desalted on a Vanguard Pre-column for 3 min. The digested peptides are sent to a BEH C18 reversed-phase column in a column / valve thermostatic compartment. A gradient solvent system consisting of solvent A: 0.1% formic acid / 99% water / 1% acetonitrile and solvent B: 0.1% formic acid / 5% water / 95% acetonitrile is utilized. The percentage of solvent B is increased from 10% to 15% in 5.1 min, to 50% in 11 min, to 90% in 11.5 min, held for 12.5 min, to 0% B in 13 min, and held for 14 min. Chromatographic separation was performed at 4°C with a flow rate of 180 μl / min. After chromatographic separation, the samples are placed into a Thermo Scientific Orbitrap Fusion mass spectrometer operated in positive electrospray ionization mode. The method used includes activated forms of CID and ETD in case of identifying control peptides, and utilizes a resolution of 120,000, a minimum signal of 10,000, an isolation width of 1.0, and a normalized collision energy of 35.0 V. The S-lens RF level is set to 60%. For control peptides, data collection type is profile for full MS scan and centroid for CID MS / MS data. For deuterated samples, no MS / MS is collected. Data is collected in the mass range of 280-1800 Da.Raw LC-MS / MS fragmentation data analysis, control samples (with CID and ETD MS / MS) are analyzed using Proteome Discover 1.4 (Thermo Scientific) and PMi Byonic (Protein Metrics) for a given sequence to generate a table of peptides and retention times. Raw data files are pre-processed and converted to ASCII format using dedicated in-house SHARC software. Identified peptides are then matched and summarized using dedicated in-house SHAFT software. Epitopes are identified by differences in the average mass shift induced by binding after deuterium labeling. At the peptide level, protection greater than 0.4 Da is considered significant.

[0116] Epitope mapping results are shown for an exemplary antibody of the present invention (ANGPT2-opt-13) (Figure 2), a nevescuumab analog, a MED3617 analog, and LC06. Nevescuumab reportedly does not cross-react with ANGPT1, while MED3617 analog and LC06 reportedly cross-react with ANGPT1. The specific binding site of the antibody to the FLD domain of human ANGPT2 having SEQ ID NO:50 (Figure 2) is highlighted in dark grey. The data show that the comparator antibodies (nevescuumab analog, MED3617, and LC06) bind to a different epitope that is distinct from the binding epitope of ANGPT2-opt-13.

[0117] Example 6 CDC assay The IgG heavy chain Fc region may have the ability to confer effector function to antibodies through interaction with C1q, thereby inducing complement-dependent cytotoxicity (CDC). This effect may be investigated in vitro by exposing target cells to complement in the presence of an antibody that specifically binds to the target cells. CDC assays can be used to assess the activity of monoclonal antibodies in mediating CDC (see "Mapping of the C1q binding site on Rituxan, a chimeric antibody with a human IgG1 Fc," Idusogie, Esohe E. et al., Journal of Immunology (2000), 164 (8), 4178-4184; and "Utilization of Complement-Dependent Cytotoxicity To Measure Low Levels of Antibodies: Application to Nonstructural Protein 1 in a Model of Japanese Encephalitis Virus," Konishi, Eiji et al., Clin Vaccine Immunol. (2008) Jan; 15(1): 88-94).

[0118] CHO cells expressing membrane-bound human ANGPT2 (CHO-GPI-ANGPT2) are cultured in RPMI-1640 supplemented with HI FBS, 1x glutamax and 1000 μg / ml geneticin (used as target cells). CDC activity is determined by measuring the release of LDH from target cells using the Cytotoxicity Detection Kit Plus from Roche®. Samples are prepared in triplicate in 96-well round-bottom plates. Samples consist of 50 μl antibody, 50 μl target cells (50,000 cells / well) and 100 μl human complement (Cedarlane®) at a final 1:12 dilution in cytotoxicity medium (Cedarlane®). Background control is 200 μl cytotoxicity medium (Cedarlane®) only. Maximum release control (Tmax) is 50 μl target cells and 150 μl cytotoxicity medium. Target cell control (Tspon) is 50 μl target cells and 150 μl cytotoxicity medium. The plate is incubated for 3 hours at 37° C. in a humidified CO2 incubator. 30 minutes before the end of the incubation (after 2.5 hours of incubation), 10 μl lysis solution (provided in the Cytotoxicity Detection Kit-Roche®) is added to the maximum release (Tmax) control wells. At the end of the incubation, 100 μl of supernatant is transferred to the corresponding wells of a 96-well flat-bottom plate for LDH detection. 100 μl of reaction mixture (provided in the Cytotoxicity Detection Kit) is added to each well and the plate is incubated at room temperature for 15 minutes in the dark. At the end of this second incubation, the reaction is stopped by the addition of 50 μl of stop solution (provided in the Cytotoxicity Detection Kit). The absorbance is measured on a Biotek® plate reader (Biotek, Synergy) at a wavelength of 490 nm with a 650 nm reference. CDC% is calculated according to the formula: %CDC=((Ab-induced release)-[(T)_spon]) / ((T_max)-(T_spon)) * 100 Calculate using:

[0119] The CDC study described above is used to determine the cytotoxicity results of the anti-ANG antibodies: ANGPT2-opt-13-IgG, nevescuumab analogs, MEDI3617 analogs, and LC06. The results of two repeated studies are shown in Figures 1A and 1B. The results show that an exemplary anti-ANGPT2 antibody of the present invention (ANGPT2-opt-13-IgG) is less cytoxic than each of the nevescuumab analogs, MEDI3617 analogs, and LC06.

[0120] Example 7 ANGPT2 blockade assay Human ANGPT2 dimers (CC-FLD) are pre-incubated with the test antibodies. The ANGPT2 / antibody mixture is then incubated with HEK293 human Tie2 cells at 4 °C. After washing, cells are stained with anti-His-AF647 (from GenScript) for detection of the His-tag on the ANGPT2 protein. Binding of secondary labeled ANGPT2 to human Tie2 cells is detected by flow cytometry. The average of duplicate values ​​at each concentration point is used to derive a curve fit graph. Antibodies that prevent ANGPT2 binding to Tie2 cells are considered blocking antibodies. The results are shown in Figures 3A-3G and EC50 values ​​are shown in Table 5 based on the average of duplicate values.

[0121] [Table 5] The results of the ANGPT2 blocking assay demonstrate that the antibodies of the invention block ANGPT2 interaction with its cell surface receptor, Tie2.

[0122] Example 8 Tie2 phosphorylation functional assay The Tie2 phosphorylation functional assay is performed as described below. Unless otherwise specified, the following reagents or materials are used: HEK293 / huTie2 cells; 0.25% trypsin / EDTA (Gibco catalog number 25200-056); Poly-D-lysine coated black, clear bottom 96 wells / plate (BioCoat Cat. No. 35460); PathScan Sandwich ELISA Lysis Buffer (Cell Signaling, catalog no. 7018); HALT protease and phosphatase inhibitor cocktail (Thermo Scientific, catalog number 78443, lot number QK226996, 100x stock); Activated sodium orthovanadate, 200 mM stock (Five photon, catalog number ActVO-4, lot number 26716-4);

[0123] clear 96-well high-binding polystyrene microplates (R&D Systems catalog number DY990, lot number 316940); ELISA blocking buffer: phosphate-buffered saline (PBS) 2% by weight BSA, diluted from a 10% stock (R&D Systems, catalog no. DY995); ELISA assay diluent: PBS 1% BSA by weight, diluted from 10% stock (R&D Systems, Cat. No. DY995); ELISA wash buffer concentrate: 25X stock (R&D Systems, Cat. No. WA126); ELISA substrate reagent pack (R&D Systems, catalog DY999); ELISA Stop Solution (R&D Systems, Cat. DY994); and rh Angiopoietin-2 (R&D systems catalog 623-AN / CF; lot number SUL61, 169ug / ml stock).

[0124] Cell plating medium: Dulbecco's modified Eagle's medium (DMEM) containing 4.5 g / l glucose and L-glutamine (500 ml) (Gibco catalog number 11995-065); Fetal bovine serum (FBS) (50 ml) (Hyclone catalogue SH30071.03, lot number AC10219630); 100 mM non-essential amino acid (NEAA) solution (5 ml) (Gibco: Catalog number 11140-050); 100 mM sodium pyruvate (5 ml) (Gibco catalog number 11360-070); PenStrep (5 ml) (Gibco catalog number 15140-122); and 1M N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid (Hepes) (6.25 ml) (Gibco catalog number 15630-080); Geneticin (10 ml) (Gibco catalog number 10131-035).

[0125] Starvation medium: DMEM containing 4,5 g / l glucose, L-glutamine; FBS (25 ml) 5%; NEAA (5 ml); Sodium pyruvate (5 ml);PenStrep (2.5 ml); Hepes (6.25 ml); and Geneticin (10 ml) Dulbecco's Phosphate Buffered Saline (dPBS), calcium and magnesium free (Gibco Catalog No. 14190).

[0126] Cell plating HEK293 / huTie2 cells are washed with PBS, detached with 0.25% trypsin, and counted using a countess cell counter (Invitrogen). 5x10 cells per well. 4Plate cells in 100 ul Dulbecco's Modified Eagle Medium (DMEM) with 10% FBS, NEAA, sodium pyruvate, PenStrep, Geneticin and HEPES in 96-well poly-D-lysine clear bottom tissue culture plates. Incubate cells overnight at 37°C in a 5% CO2 incubator. After approximately 18 hours, replace cell plating medium with 100 ul of starvation medium (DMEM) with 5% FBS, NEAA, sodium pyruvate, PenStrep, Geneticin and HEPES and return plates to the incubator to incubate overnight. ELISA Plate Coating Capture antibody (anti-Tie2 (AB33) from Cell signalling) is diluted to 1ug / ml working solution in coating buffer (eBioscience). Working solution is immediately added to 96-well high binding polystyrene microplates (R&D) at 100ul per well. Seal wells and incubate overnight at 4°C. Wash plates 3 times with 1X ELISA wash buffer using 300ul per well, then block with 200ul blocking buffer for 2 hours at room temperature with shaking.

[0127] Cell treatment Dilute the stock solution of Ang2 (rh Angiopoietin-2, from R&D systems, Cat. No. 623-AN / CF; Lot No. SUL61) to 6ug / ml in starvation medium. (Starvation medium is also used as antibody diluent). Separately, prepare a solution of anti-ANGPT2 antibody by dilution to 66nM, followed by a 1:3 serial dilution to 0.27nM. Incubate anti-ANGPT2 antibody with rhAng2 for 30 minutes at room temperature. Remove cell culture medium (50ul) from each well of the cell plate. Treat cells with 50ul aliquots of pre-incubated anti-ANGPT2 antibody solution for 20 minutes at 37°C in a 5% CO2 incubator. Discard the supernatant and wash cells once with cold PBS (containing 1mM activated sodium orthovanadate). Discard the wash buffer and add PathScan lysis buffer (Cell Signaling) containing 1 mM activated sodium orthovanadate, protease and phosphatase inhibitors to each cell. Shake the plate at fast speed for 1-3 h at 4 °C.

[0128] ELISA Blocking buffer is removed from the ELISA plate, cell lysates are added to each well, and the plate is incubated overnight at 4°C with shaking. The plate is washed 4 times with ELISA wash buffer and each well is treated with detection antibody: Biotin conjugate-4G10 platinum anti-phosphotyrosine (from Millipore), diluted 1:300 in ELISA assay diluent. The plate is incubated for 2 hours at room temperature on a shaker and then washed 4 times with ELISA wash buffer. Streptavidin-HRP conjugate (from Millipore), diluted 1:300 in ELISA assay diluent, is added to each well and incubated for 1 hour at room temperature on a shaker. The plate is washed 4 times with ELISA wash buffer. Following the wash step, substrate solution from an ELISA substrate reagent pack (R&D Systems) is added to each well for 5-10 minutes at room temperature. Stop solution is added, followed by gentle tapping for 5 minutes to mix thoroughly. The plate is then read using a plate reader at an absorbance of 450 nm corrected to 650 nm.

[0129] [Table 6] The results of the Tie2 functional assay indicate that anti-ANGPT2 antibodies can inhibit ANGPT2-induced Tie2 phosphorylation and block Tie2-mediated phosphorylation and downstream signaling.

[0130] Example 9 binding affinity Binding affinities to various ANGPT2 analytes are determined by surface plasmon resonance (SPR) using a ProteOn XPR36 (Bio-Rad). All reagents are obtained from Bio-Rad unless otherwise specified. The running buffer for all assays and dilutions (except where stated) is phosphate buffered saline (PBS) / ethylenediaminetetraacetic acid (EDTA) with 0.01% Tween 20 (PBS-T-EDTA). PBS-T-EDTA is prepared by adding 100 μl of 100% Tween 20 to 2 L of PBS-T-EDTA with an initial concentration of 0.005% Tween 20 to make a final Tween 20 concentration of 0.01%. General Linear Model (GLM) sensor chips are standardized, preconditioned, and activated with an equal mixture of 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide (EDC) / N-hydroxysulfosuccinimide (s-NHS) for 300 seconds horizontally at a flow rate of 30 μl / min. Immobilization is then performed with recombinant Protein A / G (Thermo Scientific) (60 μg / ml in 10 mM acetate pH 4.5) for 300 seconds horizontally at a flow rate of 30 μl / min, resulting in approximately 4370-4875 response units (RU) of Protein A / G on the surface. The sensor chips are then inactivated with 1 M ethanolamine HCl for 300 seconds horizontally at a flow rate of 30 μl / min. The sensor chips are stabilized with 0.85% phosphoric acid for 18 seconds at a flow rate of 100 μl / min, three times horizontally and three times vertically.

[0131] Each ANGPT2 antibody is captured vertically onto the Protein A / G surface for 300 seconds at a flow rate of 30 μl / min, resulting in a capture level of approximately 2500 RU. The baseline is stabilized by injecting PBS-T-EDTA for 60 seconds horizontally at a flow rate of 100 μl / min with a dissociation time of 120 seconds. The analyte (e.g., HuANGPT2) is injected horizontally onto the capture antibody for 300 seconds at a flow rate of 30 μl / min with a dissociation time of 1800 seconds. The analyte concentrations are 0 nM, 6.25 nM, 12.5 nM, 25 nM, 50 nM, and 100 nM. The surface is regenerated by injecting 0.85% phosphoric acid for 18 seconds at a flow rate of 100 μl / min once horizontally and once vertically. PBS-T-EDTA is injected for 60 seconds at a flow rate of 100 μl / min once vertically and once horizontally.

[0132] Interspots (interactions with the sensor surface) and blanks (PBS-T-EDTA with 0.01% Tween20 or 0 nM analyte (here HuANGPT2)) are subtracted from the raw data. The on-rate (k a ), off-rate (k d ) and affinity (K D ) Sensorgrams are fitted to 1:1 Langmuir binding to present values.

[0133] The above procedure is used to measure binding affinity to the following analytes: human ANGPT1, human ANGPT2, cynomolgus monkey (cyno) ANGPT2, rabbit ANGPT2 and rat ANGPT2. No binding is observed with human ANGPT1 (K D >100 nM). The results for binding to human ANGPT2 and cyno ANGPT2 are shown in Table 7. [Table 7]

[0134] For studies performed in a rabbit ANGPT2 model, ANGPT2-opt-13 and the nevescuumab analog had KD Indicates the value. For studies performed in a rat model, the anti-ANGPT2 antibodies of the present invention exhibited weak binding (K D ≥ 118 nM), whereas the nevescuumab analog showed a K of 0.159 nM. D Indicates the value. The results demonstrate that the anti-ANGPT2 antibodies of the invention have high affinity for human ANGPT2 and no measurable affinity for human ANGPT1.

[0135] This disclosure includes, inter alia: Embodiment 1: A heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 13 (H-CDR1); the amino acid sequence of SEQ ID NO: 15 (H-CDR2); and the amino acid sequence of SEQ ID NO: 17 (H-CDR3), and A light chain variable region comprising the amino acid sequence of SEQ ID NO:19 (L-CDR1); the amino acid sequence of SEQ ID NO:22 (L-CDR2); and the amino acid sequence of SEQ ID NO:24 (L-CDR3). or a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 14 (H-CDR1); the amino acid sequence of SEQ ID NO: 15 (H-CDR2); and the amino acid sequence of SEQ ID NO: 17 (H-CDR3); and A light chain variable region comprising the amino acid sequence of SEQ ID NO:19 (L-CDR1); the amino acid sequence of SEQ ID NO:22 (L-CDR2); and the amino acid sequence of SEQ ID NO:24 (L-CDR3). or a heavy chain variable region comprising the amino acid sequence of SEQ ID NO:13 (H-CDR1); the amino acid sequence of SEQ ID NO:15 (H-CDR2); and the amino acid sequence of SEQ ID NO:16 (H-CDR3); and A light chain variable region comprising the amino acid sequence of SEQ ID NO:20 (L-CDR1); the amino acid sequence of SEQ ID NO:23 (L-CDR2); and the amino acid sequence of SEQ ID NO:24 (L-CDR3). or a heavy chain variable region comprising the amino acid sequence of SEQ ID NO:13 (H-CDR1); the amino acid sequence of SEQ ID NO:15 (H-CDR2); and the amino acid sequence of SEQ ID NO:16 (H-CDR3); and A light chain variable region comprising the amino acid sequence of SEQ ID NO:21 (L-CDR1); the amino acid sequence of SEQ ID NO:23 (L-CDR2); and the amino acid sequence of SEQ ID NO:25 (L-CDR3). or a heavy chain variable region comprising the amino acid sequence of SEQ ID NO:13 (H-CDR1); the amino acid sequence of SEQ ID NO:15 (H-CDR2); and the amino acid sequence of SEQ ID NO:16 (H-CDR3); and A light chain variable region comprising the amino acid sequence of SEQ ID NO:20 (L-CDR1); the amino acid sequence of SEQ ID NO:22 (L-CDR2); and the amino acid sequence of SEQ ID NO:24 (L-CDR3). An anti-ANGPT2 antibody or antigen-binding fragment comprising: Embodiment 2: The anti-ANGPT2 antibody of embodiment 1, comprising a variable heavy chain and a variable light chain comprising the amino acid sequences of SEQ ID NO:3 and SEQ ID NO:8, respectively; SEQ ID NO:4 and SEQ ID NO:9, respectively; SEQ ID NO:5 and SEQ ID NO:10, respectively; SEQ ID NO:6 and SEQ ID NO:11, respectively; or SEQ ID NO:7 and SEQ ID NO:12, respectively. Embodiment 3: The anti-ANGPT2 antibody of embodiment 2, comprising a variable heavy chain and a variable light chain having at least 90%, at least 95%, at least 98%, or at least 99% identity to the amino acid sequences of SEQ ID NO:3 and SEQ ID NO:8, respectively; SEQ ID NO:4 and SEQ ID NO:9, respectively; SEQ ID NO:5 and SEQ ID NO:10, respectively; SEQ ID NO:6 and SEQ ID NO:11, respectively; or SEQ ID NO:7 and SEQ ID NO:12, respectively.

[0136] Embodiment 4: The anti-ANGPT2 antibody of embodiment 1, comprising a heavy chain and a light chain comprising SEQ ID NO:31 and SEQ ID NO:32, respectively; SEQ ID NO:33 and SEQ ID NO:34, respectively; SEQ ID NO:35 and SEQ ID NO:36, respectively; SEQ ID NO:37 and SEQ ID NO:38, respectively; or SEQ ID NO:39 and SEQ ID NO:40. Embodiment 5: The anti-ANGPT2 antibody of embodiment 4, comprising a heavy chain and a light chain having at least 90%, at least 95%, at least 98%, or at least 99% identity to the amino acid sequences of SEQ ID NO:31 and SEQ ID NO:32, respectively; SEQ ID NO:33 and SEQ ID NO:34, respectively; SEQ ID NO:35 and SEQ ID NO:36, respectively; SEQ ID NO:37 and SEQ ID NO:38, respectively; or SEQ ID NO:39 and SEQ ID NO:40. Embodiment 6: An anti-ANGTP2 antibody or antigen-binding fragment thereof according to any of embodiments 1 to 5, which binds to at least one amino acid residue of SEQ ID NO:51 or to SEQ ID NO:51. Embodiment 7: An anti-ANGTP2 antibody or antigen-binding fragment thereof that binds to at least one amino acid residue within the amino acid region of the FLD domain of human ANGPT2 comprising SEQ ID NO:51, or to SEQ ID NO:51.

[0137] Embodiment 8: An anti-ANGTP2 antibody or an antigen-binding fragment thereof that competes with the anti-ANGTP2 antibody according to any of embodiments 1 to 7 for binding to SEQ ID NO:51. Embodiment 9: An isolated polynucleotide having a sequence encoding an antibody as defined in any of embodiments 1 to 8. Embodiment 10: A vector comprising a polynucleotide according to embodiment 9. Embodiment 11: A host cell transformed or transfected with a polynucleotide according to embodiment 9 or with a vector according to embodiment 10. Embodiment 12: A method for producing an antibody according to any of embodiments 1-8, comprising: (a) culturing a host cell according to embodiment 11 under conditions allowing expression of an antibody (anbitody) according to any of embodiments 1-8, and (b) recovering the produced antibody from the culture. Embodiment 13: A pharmaceutical composition comprising an anti-ANGPT2 antibody or antigen-binding fragment according to any of embodiments 1-8 and a pharma- ceutically acceptable carrier. Embodiment 14: A pharmaceutical composition according to embodiment 13, further comprising a selected second therapeutic agent.

[0138] Embodiment 15: A pharmaceutical composition according to embodiment 13 or 14, administered by parenteral, intravenous or subcutaneous route of administration. Embodiment 16: An anti-ANGPT2 antibody or antigen-binding fragment according to any of embodiments 1 to 8 for use as a medicament. Embodiment 17: A method of treating a disease or disorder that can be alleviated by treatment with an anti-ANGPT2 antibody, comprising administering to a patient in need thereof a pharma- ceutical effective amount of an anti-ANGPT2 antibody or antigen-binding fragment according to any one of embodiments 1-8. Embodiment 18: An anti-ANGPT2 antibody or antigen-binding fragment according to any of embodiments 1-8 for use in treating a disease or disorder that can be alleviated by treatment with an anti-ANGPT2 antibody. Embodiment 19: Use of an anti-ANGPT2 antibody or antigen-binding fragment according to any of claims 1 to 8 in the manufacture of a medicament for treating a disease or disorder that can be alleviated by treatment with an anti-ANGPT2 antibody.

[0139] Embodiment 20: The disease or disorder is selected from the group consisting of cardiac hypertrophy, myocardial infarction, ischemia, ischemic reperfusion injury, hypertensive stroke, pulmonary arterial hypertension, idiopathic pulmonary arterial hypertension, trauma-induced brain damage, asthma, chronic obstructive pulmonary disease (COPD), rheumatoid arthritis, inflammatory bowel disease, multiple sclerosis, preeclampsia and pregnancy-induced hypertension, sepsis, severe sepsis, septic shock, nonalcoholic steatohepatitis (NASH), cirrhosis, minimal change syndrome, focal segmental glomerulosclerosis (FSGS), nephrotic syndrome, diabetic nephropathy (DKD), chronic kidney disease (CKD), diabetic renal failure, end stage renal disease, ischemia or ischemic reperfusion injury, cancer, hepatocellular carcinoma, idiopathic pulmonary fibrosis (IPF), emphysema, acute lung injury (ALI), acute respiratory distress syndrome (ARDS ... The method of embodiment 17, the use of the anti-ANGPT2 antibody or antigen-binding fragment of embodiment 18, or the anti-ANGPT2 antibody or antigen-binding fragment of embodiment 19, in which the ANGPT2 antibody or antigen-binding fragment is selected from the group consisting of acute respiratory syndrome (ARDS), severe acute respiratory syndrome (SARS), Middle Eastern respiratory syndrome (MERS), vascular hyperpermeability (and related disorders), acute kidney injury, renal cell carcinoma, heart failure, lupus nephritis, Raynaud's, pancreatitis, peripheral arterial disease, congenital heart disease, dengue virus, malaria, hantavirus, edema, regeneration, lupus, interstitial lung disease, scleroderma, retinopathy, diabetic nephropathy, portal hypertension, varicose vein development, and liver transplantation.

[0140] Embodiment 21: The method of embodiment 20, the anti-ANGPT2 antibody or antigen-binding fragment of embodiment 20, or the use of the anti-ANGPT2 antibody or antigen-binding fragment of embodiment 20, wherein the disease or disorder is selected from the group consisting of chronic kidney disease, nonalcoholic steatohepatitis (NASH) and sepsis. Embodiment 22: The method of embodiment 17 or 20, further comprising administering a selected second therapeutic agent. Embodiment 23: The method of embodiment 17 or 20, wherein the antibody is administered by a parenteral, intravenous or subcutaneous route of administration. Embodiment 24: A method of blocking the function of human ANGPT2 in a human patient, comprising administering to the human patient a composition comprising an anti-ANGPT2 antibody or antigen-binding fragment according to any of embodiments 1-8 in an amount sufficient to block ANGPT2-mediated responses in the human patient.

[0141] Embodiment 25: An anti-ANGPT2 antibody or antigen-binding fragment according to any of embodiments 1-8 for use in blocking the function of human ANGPT2. Embodiment 26: Use of an anti-ANGPT2 antibody or antigen-binding fragment according to any of embodiments 1 to 8 in the manufacture of a medicament for blocking the function of human ANGPT2. Embodiment 27: An isolated polynucleotide encoding a heavy chain variable region amino acid sequence or a light chain variable region, wherein the heavy chain variable region amino acid sequence comprises any one of SEQ ID NOs:3-7, 13-17; SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:35, SEQ ID NO:37, or SEQ ID NO:39, and the light chain variable region amino acid sequence comprises any one of SEQ ID NOs:8-12, 19-26, SEQ ID NO:32, SEQ ID NO:34, SEQ ID NO:36, SEQ ID NO:38, or SEQ ID NO:40.

Claims

1. a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 13 (H-CDR1); the amino acid sequence of SEQ ID NO: 15 (H-CDR2); and the amino acid sequence of SEQ ID NO: 17 (H-CDR3); and A light chain variable region comprising the amino acid sequence of SEQ ID NO:19 (L-CDR1); the amino acid sequence of SEQ ID NO:22 (L-CDR2); and the amino acid sequence of SEQ ID NO:24 (L-CDR3). or a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 14 (H-CDR1); the amino acid sequence of SEQ ID NO: 15 (H-CDR2); and the amino acid sequence of SEQ ID NO: 17 (H-CDR3); and A light chain variable region comprising the amino acid sequence of SEQ ID NO:19 (L-CDR1); the amino acid sequence of SEQ ID NO:22 (L-CDR2); and the amino acid sequence of SEQ ID NO:24 (L-CDR3). or a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 13 (H-CDR1); the amino acid sequence of SEQ ID NO: 15 (H-CDR2); and the amino acid sequence of SEQ ID NO: 16 (H-CDR3); and A light chain variable region comprising the amino acid sequence of SEQ ID NO:20 (L-CDR1); the amino acid sequence of SEQ ID NO:23 (L-CDR2); and the amino acid sequence of SEQ ID NO:24 (L-CDR3). or a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 13 (H-CDR1); the amino acid sequence of SEQ ID NO: 15 (H-CDR2); and the amino acid sequence of SEQ ID NO: 16 (H-CDR3); and A light chain variable region comprising the amino acid sequence of SEQ ID NO:21 (L-CDR1); the amino acid sequence of SEQ ID NO:23 (L-CDR2); and the amino acid sequence of SEQ ID NO:25 (L-CDR3). or a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 13 (H-CDR1); the amino acid sequence of SEQ ID NO: 15 (H-CDR2); and the amino acid sequence of SEQ ID NO: 16 (H-CDR3); and A light chain variable region comprising the amino acid sequence of SEQ ID NO:20 (L-CDR1); the amino acid sequence of SEQ ID NO:22 (L-CDR2); and the amino acid sequence of SEQ ID NO:24 (L-CDR3). An anti-ANGPT2 antibody or antigen-binding fragment comprising:

2. The anti-ANGPT2 antibody of claim 1, comprising a variable heavy chain and a variable light chain comprising the amino acid sequences of SEQ ID NO:3 and SEQ ID NO:8, respectively; SEQ ID NO:4 and SEQ ID NO:9, respectively; SEQ ID NO:5 and SEQ ID NO:10, respectively; SEQ ID NO:6 and SEQ ID NO:11, respectively; or SEQ ID NO:7 and SEQ ID NO:12, respectively.

3. The anti-ANGPT2 antibody of claim 1, comprising a heavy chain and a light chain comprising SEQ ID NO:31 and SEQ ID NO:32, respectively; SEQ ID NO:33 and SEQ ID NO:34, respectively; SEQ ID NO:35 and SEQ ID NO:36, respectively; SEQ ID NO:37 and SEQ ID NO:38, respectively; or SEQ ID NO:39 and SEQ ID NO:

40.

4. A pharmaceutical composition comprising the anti-ANGPT2 antibody or antigen-binding fragment according to any one of claims 1 to 3, and optionally a pharma- ceutically acceptable carrier.

5. The pharmaceutical composition according to claim 4 for treating a disease or disorder that can be alleviated by treatment with the anti-ANGPT2 antibody or antigen-binding fragment according to any one of claims 1 to 3.

6. 4. Use of the anti-ANGPT2 antibody or antigen-binding fragment according to any one of claims 1 to 3 in the manufacture of a medicament for treating a disease or disorder that can be alleviated by treatment with an anti-ANGPT2 antibody.

7. The disease or disorder is cardiac hypertrophy, myocardial infarction, ischemia, ischemia-reperfusion injury, hypertensive stroke, hypertension), pulmonary arterial hypertension, idiopathic pulmonary arterial hypertension, trauma-induced brain damage, asthma, chronic obstructive pulmonary disease (COPD), rheumatoid arthritis, inflammatory bowel disease, multiple sclerosis, preeclampsia and pregnancy-induced hypertension, sepsis, severe sepsis, septic shock, nonalcoholic steatohepatitis (NASH), cirrhosis, minimal change disease, focal segmental glomerulosclerosis (FSGS), nephrotic syndrome, diabetic nephropathy (DKD), chronic kidney disease (CKD), diabetic renal failure, end stage renal disease, ischemia or ischemic reperfusion injury, cancer, hepatocellular carcinoma, idiopathic pulmonary fibrosis (IPF), emphysema, acute lung injury (ALI), acute respiratory distress syndrome (ACD) 6. The pharmaceutical composition of claim 5, wherein the therapeutic agent is selected from the group consisting of acute respiratory syndrome (ARDS), severe acute respiratory syndrome (SARS), Middle Eastern respiratory syndrome (MERS), vascular hyperpermeability (and related disorders), acute kidney injury, renal cell carcinoma, heart failure, lupus nephritis, Raynaud's, pancreatitis, peripheral arterial disease, congenital heart disease, dengue virus, malaria, hantavirus, edema, regeneration, lupus, interstitial lung disease, scleroderma, retinopathy, diabetic nephropathy, portal hypertension, varices growth and liver transplantation.

8. 8. The pharmaceutical composition of claim 7, wherein the disease or disorder is selected from the group consisting of chronic kidney disease, nonalcoholic steatohepatitis (NASH), diabetic nephropathy, sepsis and vascular hyperpermeability.

9. 8. The pharmaceutical composition of claim 7, wherein the disease or disorder is selected from the group consisting of ALI, ARDS, SARS, MERS and vascular hyperpermeability (and related disorders).

10. The disease or disorder is cardiac hypertrophy, myocardial infarction, ischemia, ischemia-reperfusion injury, hypertensive stroke, hypertension), pulmonary arterial hypertension, idiopathic pulmonary arterial hypertension, trauma-induced brain damage, asthma, chronic obstructive pulmonary disease (COPD), rheumatoid arthritis, inflammatory bowel disease, multiple sclerosis, preeclampsia and pregnancy-induced hypertension, sepsis, severe sepsis, septic shock, nonalcoholic steatohepatitis (NASH), cirrhosis, minimal change disease, focal segmental glomerulosclerosis (FSGS), nephrotic syndrome, diabetic nephropathy (DKD), chronic kidney disease (CKD), diabetic renal failure, end stage renal disease, ischemia or ischemic reperfusion injury, cancer, hepatocellular carcinoma, idiopathic pulmonary fibrosis (IPF), emphysema, acute lung injury (ALI), acute respiratory distress syndrome (ACD) 7. The use of the anti-ANGPT2 antibody or antigen-binding fragment of claim 6, wherein the antibody or antigen-binding fragment is selected from the group consisting of acute respiratory syndrome (ARDS), severe acute respiratory syndrome (SARS), Middle Eastern respiratory syndrome (MERS), vascular hyperpermeability (and related disorders), acute kidney injury, renal cell carcinoma, heart failure, lupus nephritis, Raynaud's, pancreatitis, peripheral arterial disease, congenital heart disease, dengue virus, malaria, hantavirus, edema, regeneration, lupus, interstitial lung disease, scleroderma, retinopathy, diabetic nephropathy, portal hypertension, varices growth, and liver transplantation.

11. The use of the anti-ANGPT2 antibody or antigen-binding fragment of claim 10, wherein the disease or disorder is selected from the group consisting of chronic kidney disease, nonalcoholic steatohepatitis (NASH), diabetic nephropathy, sepsis and increased vascular permeability.

12. The use of an anti-ANGPT2 antibody or antigen-binding fragment according to claim 10, wherein the disease or disorder is selected from the group consisting of ALI, ARDS, SARS, MERS and vascular hyperpermeability (and related disorders).

13. The pharmaceutical composition of claim 5 or 7, which is administered in combination with a second therapeutic agent.

14. 8. The pharmaceutical composition of claim 5 or 7, which is administered by parenteral, intravenous or subcutaneous administration route.

15. The pharmaceutical composition according to claim 4 for blocking the function of human ANGPT2 in a human patient.

16. Use of the anti-ANGPT2 antibody or antigen-binding fragment according to any one of claims 1 to 3 in the manufacture of a medicament for blocking the function of human ANGPT2.

17. A plurality of polynucleotides encoding an anti-ANGPT2 antibody or antigen-binding fragment, comprising an isolated polynucleotide encoding a heavy chain variable region amino acid sequence and an isolated polynucleotide encoding a light chain variable region amino acid sequence, the heavy chain variable region comprises the amino acid sequence of SEQ ID NO: 13 (H-CDR1); the amino acid sequence of SEQ ID NO: 15 (H-CDR2); and the amino acid sequence of SEQ ID NO: 17 (H-CDR3); and The light chain variable region comprises the amino acid sequence of SEQ ID NO: 19 (L-CDR1); the amino acid sequence of SEQ ID NO: 22 (L-CDR2); and the amino acid sequence of SEQ ID NO: 24 (L-CDR3). or the heavy chain variable region comprises the amino acid sequence of SEQ ID NO: 14 (H-CDR1); the amino acid sequence of SEQ ID NO: 15 (H-CDR2); and the amino acid sequence of SEQ ID NO: 17 (H-CDR3); and The light chain variable region comprises the amino acid sequence of SEQ ID NO: 19 (L-CDR1); the amino acid sequence of SEQ ID NO: 22 (L-CDR2); and the amino acid sequence of SEQ ID NO: 24 (L-CDR3). or the heavy chain variable region comprises the amino acid sequence of SEQ ID NO: 13 (H-CDR1); the amino acid sequence of SEQ ID NO: 15 (H-CDR2); and the amino acid sequence of SEQ ID NO: 16 (H-CDR3); and The light chain variable region comprises the amino acid sequence of SEQ ID NO:20 (L-CDR1); the amino acid sequence of SEQ ID NO:23 (L-CDR2); and the amino acid sequence of SEQ ID NO:24 (L-CDR3). or the heavy chain variable region comprises the amino acid sequence of SEQ ID NO: 13 (H-CDR1); the amino acid sequence of SEQ ID NO: 15 (H-CDR2); and the amino acid sequence of SEQ ID NO: 16 (H-CDR3); and The light chain variable region comprises the amino acid sequence of SEQ ID NO:21 (L-CDR1); the amino acid sequence of SEQ ID NO:23 (L-CDR2); and the amino acid sequence of SEQ ID NO:25 (L-CDR3). or the heavy chain variable region comprises the amino acid sequence of SEQ ID NO: 13 (H-CDR1); the amino acid sequence of SEQ ID NO: 15 (H-CDR2); and the amino acid sequence of SEQ ID NO: 16 (H-CDR3); and the light chain variable region comprises the amino acid sequence of SEQ ID NO: 20 (L-CDR1); the amino acid sequence of SEQ ID NO: 22 (L-CDR2); and the amino acid sequence of SEQ ID NO: 24 (L-CDR3); The plurality of polynucleotides.

18. 18. The multiple polynucleotides of claim 17, wherein the heavy chain variable region and the light chain variable region comprise the amino acid sequences of SEQ ID NO:3 and SEQ ID NO:8, respectively; SEQ ID NO:4 and SEQ ID NO:9, respectively; SEQ ID NO:5 and SEQ ID NO:10, respectively; SEQ ID NO:6 and SEQ ID NO:11, respectively; or SEQ ID NO:7 and SEQ ID NO:12, respectively.

19. A plurality of polynucleotides comprising an isolated polynucleotide encoding a heavy chain amino acid sequence and an isolated polynucleotide encoding a light chain amino acid sequence. the heavy chain amino acid sequence comprises SEQ ID NO:31 and the light chain amino acid sequence comprises SEQ ID NO:32; or the heavy chain amino acid sequence comprises SEQ ID NO:33 and the light chain amino acid sequence comprises SEQ ID NO:34; or the heavy chain amino acid sequence comprises SEQ ID NO:35 and the light chain amino acid sequence comprises SEQ ID NO:36; or the heavy chain amino acid sequence comprises SEQ ID NO:37 and the light chain amino acid sequence comprises SEQ ID NO:38; or the heavy chain amino acid sequence comprises SEQ ID NO:39, and the light chain amino acid sequence comprises SEQ ID NO:40; The plurality of polynucleotides.

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