Methods and compositions for stimulating an immune response

Abstract

The present invention relates to methods and compositions for stimulating an immune response. In particular, the present invention relates to immunostimulatory RNA molecules comprising sequences derived from an RNA molecule encoding an influenza A virus nucleoprotein, which act as adjuvants and / or immunostimulants to enhance a host's immune response. [Selected Figure] Figure 1

Description

[Technical field]

[0001] The present invention relates to methods and compositions for stimulating an immune response. In particular, the present invention relates to immunostimulatory RNA molecules comprising sequences derived from an RNA molecule encoding an influenza A virus nucleoprotein, which act as adjuvants and / or immunostimulants to enhance the host immune response. [Background technology]

[0002] The immune system plays a key role in defense against microorganisms such as viruses, fungi, and bacteria, as well as in the recognition and repelling of malignant cells (tumor cells). The evolution of the immune system has resulted in a highly effective network based on two types of defense: innate and adaptive immunity. In contrast to the evolutionarily ancient innate immune system, which relies on invariant receptors that recognize common molecular patterns associated with pathogens, adaptive immunity is based on highly specific antigen receptors and clonal selection on B cells (B lymphocytes) and T cells (T lymphocytes). B cells generate humoral immune responses by secreting antibodies, whereas T cells mediate cellular immune responses that lead to the destruction of recognized cells.

[0003] Antigen-specific immunotherapy aims to enhance or induce a specific immune response in a patient to control infectious or malignant disease. The identification of an increasing number of pathogen-associated and tumor-associated antigens has led to a wide collection of targets suitable for immunotherapy. Vaccination and immunization involve the introduction of non-toxic antigens to a subject, which trigger the subject's immune system to initiate an immune response. In many cases, vaccine antigens are inactivated or attenuated forms of disease-causing microorganisms. Various antigen forms can be used for vaccination, including whole diseased cells, proteins, peptides, or immunization vectors, such as RNA, DNA, or viral vectors that can be directly applied in vitro or in vivo by pulsing DCs after transfer to the patient. However, antigens are often not sufficiently immunogenic by themselves and do not elicit a sufficient immune response.

[0004] The immunogenicity of an antigen can be increased by administering the antigen in combination with one or more adjuvants. Adjuvants increase the response to the antigen by acting directly on the immune system or by modifying the pharmacokinetic properties of the antigen, increasing the interaction time between the antigen and the immune system. In addition, the addition of an adjuvant allows the use of smaller doses of the antigen to stimulate a similar immune response, thereby reducing the cost of producing the vaccine.

[0005] Many compounds that exhibit adjuvant activity have been described, although these adjuvants vary in efficacy, sometimes are not strong enough to induce immune responses of the desired strength, and some have limited use in humans due to their toxic effects.

[0006] Therefore, there is a need for effective adjuvant systems to improve the efficacy and safety of existing and future vaccines. Summary of the Invention

[0007] The present invention is based, at least in part, on the identification of immunostimulatory RNA molecules that contain sequences derived from an RNA molecule encoding an influenza A virus nucleoprotein, which act as adjuvants or immunostimulants to enhance the host immune response. These immunostimulatory RNA molecules can be used as immunostimulants in vivo.

[0008] As described herein, immunostimulatory RNA molecules comprising sequences derived from an RNA molecule encoding an influenza A virus nucleoprotein have been shown to induce high levels of IFN-α expression in vitro and in vivo, and strong specific B and T cell responses in vivo. Thus, these immunostimulatory RNA molecules are potent adjuvants for vaccination and are useful in methods and compositions for stimulating an immune response in a subject. One embodiment of the invention includes administering to a subject the immunostimulatory RNA molecules described herein together with one or more antigens, e.g., an antigen contained in a vaccine, to enhance or promote an antigen-specific immune response.

[0009] Thus, in one aspect, the invention provides a method for stimulating an immune response in a subject, comprising providing at least one antigen to a subject and providing an immunostimulatory RNA molecule, wherein the immunostimulatory RNA molecule comprises a sequence derived from an RNA molecule encoding an influenza A virus nucleoprotein.

[0010] In one embodiment, the sequence derived from an RNA molecule encoding an influenza A virus nucleoprotein comprises at least a fragment of an RNA molecule encoding an influenza A virus nucleoprotein or a variant thereof.

[0011] In a different embodiment, the sequence derived from the RNA molecule encoding the influenza A virus nucleoprotein or at least a fragment of the RNA molecule encoding the influenza A virus nucleoprotein, or a variant thereof, is selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, and SEQ ID NO:11.

[0012] In a further aspect, the invention provides a method for stimulating an immune response in a subject comprising providing at least one antigen to a subject and providing an immunostimulatory RNA molecule, wherein the immunostimulatory RNA molecule comprises the sequence of SEQ ID NO: 1 or a variant thereof. In one embodiment, the immunostimulatory RNA molecule comprises the sequence of SEQ ID NO: 2 or a variant thereof.

[0013] In one embodiment, the immunostimulatory RNA molecule further comprises the sequence of SEQ ID NO: 3, or a variant thereof. In one embodiment, the immunostimulatory RNA molecule further comprises the sequence of SEQ ID NO: 4, or a variant thereof.

[0014] In one embodiment, the immunostimulatory RNA molecule comprises the sequence of SEQ ID NO:5 or a variant thereof.

[0015] In one embodiment, the immunostimulatory RNA molecule comprises the sequence of SEQ ID NO: 6 or a variant thereof. In one embodiment, the immunostimulatory RNA molecule comprises the sequence of SEQ ID NO: 7 or a variant thereof.

[0016] In one embodiment, the immunostimulatory RNA molecule comprises the sequence of SEQ ID NO: 8 or a variant thereof. In one embodiment, the immunostimulatory RNA molecule comprises the sequence of SEQ ID NO: 9 or a variant thereof.

[0017] In one embodiment, the immunostimulatory RNA molecule comprises the sequence of SEQ ID NO: 10 or a variant thereof. In one embodiment, the immunostimulatory RNA molecule comprises the sequence of SEQ ID NO: 11 or a variant thereof.

[0018] In one embodiment of all aspects of the invention, the immunostimulatory RNA molecule is capable of inducing an antigen-specific immune response in a subject.

[0019] In one embodiment of all aspects of the invention, the immune response comprises a B cell response.

[0020] In one embodiment of all aspects of the invention, the immune response comprises the production of IgG antibodies, which are associated with a Th1-like response.

[0021] In one embodiment of all aspects of the invention, the immunostimulatory RNA molecule is a Toll-like receptor (TLR) agonist. In one embodiment, the TLR is TLR7.

[0022] In one embodiment of all aspects of the invention, the immunostimulatory RNA molecule is capable of inducing the secretion of interferon alpha. In one embodiment, the secretion of interferon alpha involves plasmacytoid dendritic cells.

[0023] In one embodiment of all aspects of the invention, the immunostimulatory RNA molecule does not substantially induce secretion of one or more of tumor necrosis factor alpha, interferon gamma, and interleukin-10.

[0024] In one embodiment of all aspects of the invention, the at least one antigen is selected from the group consisting of a cancer, viral, bacterial, fungal, or parasitic antigen.

[0025] In one embodiment of all aspects of the invention, the subject is a mammal. In one embodiment of all aspects of the invention, the subject is a human.

[0026] In a further aspect, the invention provides a pharmaceutical composition comprising an immunostimulatory RNA molecule, at least one antigen, and a pharma- ceutically acceptable carrier, wherein the immunostimulatory RNA molecule comprises a sequence derived from an RNA molecule encoding an influenza A virus nucleoprotein.

[0027] In one embodiment, the sequence derived from an RNA molecule encoding an influenza A virus nucleoprotein comprises at least a fragment of an RNA molecule encoding an influenza A virus nucleoprotein or a variant thereof.

[0028] In a different embodiment, the sequence derived from the RNA molecule encoding the influenza A virus nucleoprotein or at least a fragment of the RNA molecule encoding the influenza A virus nucleoprotein, or a variant thereof, is selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, and SEQ ID NO:11.

[0029] In a further aspect, the invention provides a pharmaceutical composition comprising an immunostimulatory RNA molecule, at least one antigen, and a pharma- ceutically acceptable carrier, wherein the immunostimulatory RNA molecule comprises the sequence of SEQ ID NO: 1, or a variant thereof. In one embodiment, the immunostimulatory RNA molecule comprises the sequence of SEQ ID NO: 2, or a variant thereof.

[0030] In one embodiment, the immunostimulatory RNA molecule further comprises the sequence of SEQ ID NO: 3, or a variant thereof. In one embodiment, the immunostimulatory RNA molecule further comprises the sequence of SEQ ID NO: 4, or a variant thereof.

[0031] In one embodiment, the immunostimulatory RNA molecule comprises the sequence of SEQ ID NO:5 or a variant thereof.

[0032] In one embodiment, the immunostimulatory RNA molecule comprises the sequence of SEQ ID NO: 6 or a variant thereof. In one embodiment, the immunostimulatory RNA molecule comprises the sequence of SEQ ID NO: 7 or a variant thereof.

[0033] In one embodiment, the immunostimulatory RNA molecule comprises the sequence of SEQ ID NO: 8 or a variant thereof. In one embodiment, the immunostimulatory RNA molecule comprises the sequence of SEQ ID NO: 9 or a variant thereof.

[0034] In one embodiment, the immunostimulatory RNA molecule comprises the sequence of SEQ ID NO: 10 or a variant thereof. In one embodiment, the immunostimulatory RNA molecule comprises the sequence of SEQ ID NO: 11 or a variant thereof.

[0035] In one embodiment of the pharmaceutical composition of all aspects of the invention, the immunostimulatory RNA molecule is a Toll-like receptor (TLR) agonist. In one embodiment, the TLR is TLR7.

[0036] In one embodiment of the pharmaceutical composition of all aspects of the invention, the at least one antigen is selected from the group consisting of a cancer, viral, bacterial, fungal, or parasitic antigen.

[0037] The pharmaceutical compositions described herein may be in the form of a vaccine, which may be a therapeutic or prophylactic vaccine.

[0038] In a further aspect, the present invention provides an isolated RNA molecule comprising a sequence derived from an RNA molecule encoding an influenza A virus nucleoprotein, the isolated RNA molecule having immunostimulatory activity.

[0039] In one embodiment, the sequence derived from an RNA molecule encoding an influenza A virus nucleoprotein comprises at least a fragment of an RNA molecule encoding an influenza A virus nucleoprotein or a variant thereof.

[0040] In a different embodiment, the sequence derived from the RNA molecule encoding the influenza A virus nucleoprotein or at least a fragment of the RNA molecule encoding the influenza A virus nucleoprotein, or a variant thereof, is selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, and SEQ ID NO:11.

[0041] In a further aspect, the present invention provides an isolated RNA molecule comprising the sequence of SEQ ID NO:1 or a variant thereof, the isolated RNA molecule having immunostimulatory activity. In one embodiment, the isolated RNA molecule comprises the sequence of SEQ ID NO:2 or a variant thereof.

[0042] In one embodiment, the isolated RNA molecule further comprises the sequence of SEQ ID NO: 3, or a variant thereof. In one embodiment, the isolated RNA molecule further comprises the sequence of SEQ ID NO: 4, or a variant thereof.

[0043] In one embodiment, the isolated RNA molecule comprises the sequence of SEQ ID NO:5 or a variant thereof.

[0044] In one embodiment, the isolated RNA molecule comprises the sequence of SEQ ID NO: 6 or a variant thereof. In one embodiment, the isolated RNA molecule comprises the sequence of SEQ ID NO: 7 or a variant thereof.

[0045] In one embodiment, the isolated RNA molecule comprises the sequence of SEQ ID NO: 8 or a variant thereof. In one embodiment, the isolated RNA molecule comprises the sequence of SEQ ID NO: 9 or a variant thereof.

[0046] In one embodiment, the isolated RNA molecule comprises the sequence of SEQ ID NO: 10 or a variant thereof. In one embodiment, the isolated RNA molecule comprises the sequence of SEQ ID NO: 11 or a variant thereof.

[0047] In one embodiment of the isolated RNA molecule of all aspects of the invention, the isolated RNA molecule is a Toll-like receptor (TLR) agonist. In one embodiment, the TLR is TLR7.

[0048] In one embodiment of the invention, the immunostimulatory RNA molecules or isolated RNA molecules described herein are not translatable, ie, are not templates for the production of peptides or proteins.

[0049] Another aspect relates to a method for stimulating an immune response in a subject, comprising administering to the subject a pharmaceutical composition provided according to the present invention.

[0050] In a further aspect, the invention provides agents and compositions as described herein for use in the therapeutic methods described herein, particularly for stimulating an immune response.

[0051] Other features and advantages of the invention will become apparent from the following detailed description and claims. [Brief description of the drawings]

[0052] [Figure 1] Schematic diagram of the sequential fragmentation strategy applied using influenza NP-encoding RNA (2-2-8, NP1-1565) as starting sequence [Figure 2A-B] Synthesis of isRNA by in vitro transcription, composition and sequence of isRNA, and quality control after purification [Figure 2C-D] Same as above [Figure 3A] Cytokine induction in human PBMCs by formulated isRNA [Figure 3B] Same as above [Figure 4]pDCs are the major effector cells for IFN-α secretion upon stimulation with isRNA [Diagram 5] Dependence of isRNA NP71-Seq45-mediated IFN-α induction in human PBMCs on endosome-located TLRs [Figure 6] Determination of nucleotide-sensing endosomal TLRs activated by NP71-Seq45 [Figure 7] Analysis of cytokine profiles induced in human PBMCs by formulated isRNA [Figure 8] Cytokine induction in mouse cells by formulated isRNA [Figure 9] Time- and dose-dependent induction of IFN-α in vivo by formulated isRNA [Figure 10] Repeated intravenous administration of formulated isRNA at frequent intervals generates systemic TLR response tolerance that can be overcome by adaptive immunization regimes [Figure 11A] Formulated isRNA in combination with HBcAg-#A79 VLP induces antigen-specific B and T cell responses in vivo [Figure 11B] Same as above [Figure 12A] Dose-dependent induction of antigen-specific B and T cell responses by immunization with formulated isRNA in combination with HBcAg-#A79 VLP [Figure 12B] Same as above [Figure 13] Antigen-specific antibodies elicited by immunization with formulated isRNA and HBcAg-#A79VLPs kill target-positive cells by CDC [Figure 14A-B] Immunization with formulated isRNA in combination with HBcAg-#A79VLP results in a balanced antigen-specific IgG2a / IgG1 response [Figure 14C] Same as above [Figure 15] Dependence of antigen-specific antibody responses induced by formulated isRNA in combination with HBcAg-#A79VLP on pDCs [Figure 16]Cytokine induction by F12-formulated isRNA in vivo DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0053] Although the present invention is described in detail below, it should be understood that the invention is not limited to the specific methodology, protocols, and reagents described herein, as these may vary. It should also be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present invention, which is limited only by the appended claims. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art.

[0054] The elements of the present invention are described below. Although these elements are listed with specific embodiments, it is understood that they can be combined in any manner and in any number to create additional embodiments. The various described examples and preferred embodiments should not be construed as limiting the invention to only the explicitly described embodiments. The description should be understood to support and encompass embodiments combining the explicitly described embodiments with any number of the disclosed and / or preferred elements. Furthermore, any permutation and combination of all described elements in this application should be considered to be disclosed by the description of this application unless the context indicates otherwise.

[0055] Preferably, the terms used herein are defined as set forth in "A multilingual glossary of biotechnological terms: (IUPAC Recommendations)", HGW Leuenberger, B. Nagel, and H. Kolbl, eds. (1995) Helvetica Chimica Acta, CH-4010 Basel, Switzerland.

[0056] The practice of the present invention employs, unless otherwise indicated, conventional methods of biochemistry, cell biology, immunology, and recombinant DNA technology described in the literature of the art (see, e.g., Molecular Cloning: A Laboratory Manual, 2nd Edition, eds. J. Sambrook et al., Cold Spring Harbor Laboratory Press, Cold Spring Harbor 1989).

[0057] Throughout this specification and the claims that follow, unless the context otherwise requires, the word "comprise" and variants such as "comprises" and "comprising" are understood to mean the inclusion of a stated member, integer or step, or group of members, integers or steps, and not the exclusion of any other member, integer or step, or group of members, integers or steps, although in some embodiments such other members, integers or steps, or group of members, integers or steps, may be excluded, i.e., the subject matter consists of the inclusion of a stated member, integer or step, or group of members, integers or steps. The terms "a" and "an" and "the" and similar references used in the context of describing the invention (particularly in the context of the claims) are to be construed to cover both the singular and the plural, unless otherwise stated herein or clearly contradicted by the context. The recitation of ranges of values ​​herein is merely intended to be a shorthand way of individually referring to each separate value falling within the range. Unless otherwise stated herein, each individual value is incorporated into the specification as if it were individually set forth herein.

[0058] All methods described herein can be performed in any suitable order unless otherwise indicated herein or clearly contradicted by context. The use of any and all examples or exemplary language (e.g., "etc.") provided herein is intended merely to better illustrate the invention and does not limit the scope of the invention as recited in the claims. No language in the specification should be construed as indicating any non-claimed element essential to the practice of the invention.

[0059] Several documents are cited throughout the text of this specification. Each document cited herein (including all patents, patent applications, scientific publications, manufacturer's specifications, instructions, etc.), whether supra or infra, is hereby incorporated by reference in its entirety. Nothing herein should be construed as an admission that the invention is not entitled to antedate such disclosure by virtue of prior invention.

[0060] The present invention contemplates the treatment or prevention of a disease or disorder by stimulating an immune response to an antigen associated with the disease or disorder, which immune response to the antigen is enhanced by administering the vaccine antigen or a nucleic acid encoding the vaccine antigen together with one or more immunostimulatory RNA molecules described herein, which act as an adjuvant.

[0061] The term "adjuvant" relates to a compound that prolongs or enhances or promotes an immune response when administered to an individual in combination with an antigen. Adjuvants are postulated to exert their biological activity by one or more mechanisms, including increasing the surface area of ​​the antigen, prolonging the retention of the antigen in the body, delaying antigen release, targeting the antigen to macrophages, increasing antigen uptake, enhancing antigen processing, stimulating cytokine release, stimulating and activating immune cells such as B cells, macrophages, dendritic cells, T cells, and non-specific activation of immune cells.

[0062] The present invention describes immunostimulatory RNA molecules comprising sequences derived from an RNA molecule encoding influenza A virus nucleoprotein that act as adjuvants and / or immunostimulants to enhance the host immune response.

[0063] The term "RNA molecule encoding influenza A virus nucleoprotein" relates to an RNA molecule encoding the nucleoprotein (NP) or nucleocapsid protein of influenza A virus.

[0064] Influenza A viruses have a genome that contains eight segments of RNA that code for ten identified polypeptides. Nine of these polypeptides are incorporated into the virion. Three viral polypeptides are inserted into the lipid envelope: the hemagglutinin (HA) and neuraminidase glycoproteins, involved in cell entry and exit, respectively, and M2, a low-content ion channel involved in uncoating and HA maturation. Underneath the membrane is the matrix or M1 protein, the main structural component of the virion that is thought to act as an adaptor between the lipid envelope and the internal RNP particle and is probably the driving force behind virus budding. Inside the shell of M1 are the RNPs: these contain the genomic RNA segments associated with the trimeric RNA polymerase (PB1, PB2, PA subunits) and a stoichiometric amount of NP. Small amounts of NEP / NS2 polypeptides are also found in virions.

[0065] In one embodiment, the term "RNA molecule encoding influenza A virus nucleoprotein" relates to the nucleic acid sequence of SEQ ID NO: 12 of the sequence listing or a variant thereof.

[0066] In one embodiment, the immunostimulatory RNA molecule described herein comprises the sequence of SEQ ID NO: 1 or a variant thereof. In one embodiment, the immunostimulatory RNA molecule comprises the sequence of SEQ ID NO: 2 or a variant thereof.

[0067] In one embodiment, the immunostimulatory RNA molecule further comprises the sequence of SEQ ID NO: 3, or a variant thereof. In one embodiment, the immunostimulatory RNA molecule further comprises the sequence of SEQ ID NO: 4, or a variant thereof.

[0068] In one embodiment, the immunostimulatory RNA molecule described herein comprises the sequence of SEQ ID NO:5 or a variant thereof.

[0069] In one embodiment, the immunostimulatory RNA molecule described herein comprises the sequence of SEQ ID NO: 6, or a variant thereof. In one embodiment, the immunostimulatory RNA molecule comprises the sequence of SEQ ID NO: 7, or a variant thereof.

[0070] In one embodiment, the immunostimulatory RNA molecule described herein comprises the sequence of SEQ ID NO: 8, or a variant thereof. In one embodiment, the immunostimulatory RNA molecule comprises the sequence of SEQ ID NO: 9, or a variant thereof.

[0071] In one embodiment, the immunostimulatory RNA molecule described herein comprises the sequence of SEQ ID NO: 10, or a variant thereof. In one embodiment, the immunostimulatory RNA molecule comprises the sequence of SEQ ID NO: 11, or a variant thereof.

[0072] The immunostimulatory RNA molecules described herein, when administered in combination with these antigens, induce an immune response against the antigens. In one embodiment, the immune response comprises a B cell response. In one embodiment, the immune response comprises the production of IgG antibodies.

[0073] In one embodiment, the immunostimulatory RNA molecules described herein are capable of inducing the secretion of interferon alpha. In one embodiment, the secretion of interferon alpha involves plasmacytoid dendritic cells.

[0074] In one embodiment, the immunostimulatory RNA molecules described herein do not substantially induce secretion of one or more of tumor necrosis factor alpha, interferon gamma, and interleukin-10.

[0075] In one embodiment, the immunostimulatory RNA molecules described herein are recombinant molecules. In one embodiment, the immunostimulatory RNA molecules described herein are obtained by in vitro transcription.

[0076] In various embodiments, the immunostimulatory RNA molecules described herein have a length of 20 to 400 nucleotides, 20 to 200 nucleotides, 20 to 100 nucleotides, in particular 30 to 200 nucleotides or 30 to 100 nucleotides.

[0077] According to the present invention, it is preferred to administer the immunostimulatory RNA molecules described herein formulated in a carrier or delivery vehicle, such as a nanoparticle formulation, in particular a lipoplex formulation. Thus, the immunostimulatory RNA molecules described herein may be present formulated in a carrier or delivery vehicle, such as a nanoparticle or nanoparticle formulation, in particular a lipoplex formulation, as described herein.

[0078] In one embodiment, a delivery vehicle can be used that delivers immunostimulatory RNA molecules to antigen-presenting cells, such as dendritic cells (DCs), in the spleen after systemic administration. For example, nanoparticle RNA formulations of defined particle size with zero or near-negative net particle charge, such as neutral or negatively charged lipoplexes derived from RNA and liposomes, such as lipoplexes containing DOTMA and DOPE or DOTMA and cholesterol, result in substantial delivery of RNA to splenic DCs after systemic administration. Particularly preferred in the present invention are nanoparticle RNA formulations in which the charge ratio of positive to negative charges in the nanoparticles is 1.4:1 or less and / or the zeta potential of the nanoparticles is 0 or less. In one embodiment, the charge ratio of positive to negative charges in the nanoparticles is between 1.4:1 and 1:8, preferably between 1.2:1 and 1:4, such as 1:1 and 1:3, such as 1:1.2 to 1:2, 1:1.2 to 1:1.8, 1:1.3 to 1:1.7, in particular 1:1.4 to 1:1.6, such as about 1:1.5. In one embodiment, the zeta potential of the nanoparticles is -5 or less, -10 or less, -15 or less, -20 or less or -25 or less. In various embodiments, the zeta potential of the nanoparticles is -35 or more, -30 or more or -25 or more. In one embodiment, the nanoparticles have a zeta potential of 0 mV to -50 mV, preferably 0 mV to -40 mV or -10 mV to -30 mV. In one embodiment, the positive charge is contributed by at least one cationic lipid present in the nanoparticle and the negative charge is contributed by RNA. In one embodiment, the nanoparticles comprise at least one helper lipid. The helper lipid can be a neutral or anionic lipid.

[0079] In one embodiment, the nanoparticles are lipoplexes comprising DOTMA and DOPE in a molar ratio of 10:0 to 1:9, preferably 8:2 to 3:7, more preferably 7:3 to 5:5, and the charge ratio of the positive charges of DOTMA to the negative charges of the RNA is 1.8:2 to 0.8:2, more preferably 1.6:2 to 1:2, even more preferably 1.4:2 to 1.1:2, even more preferably about 1.2:2.

[0080] In one embodiment, the nanoparticles are lipoplexes comprising DOTMA and cholesterol in a molar ratio of 10:0 to 1:9, preferably 8:2 to 3:7, more preferably 7:3 to 5:5, and the charge ratio of the positive charges of DOTMA to the negative charges of the RNA is 1.8:2 to 0.8:2, more preferably 1.6:2 to 1:2, even more preferably 1.4:2 to 1.1:2, and even more preferably about 1.2:2.

[0081] In one embodiment, the nanoparticles are lipoplexes comprising DOTAP and DOPE in a molar ratio of 10:0 to 1:9, preferably 8:2 to 3:7, more preferably 7:3 to 5:5, and the charge ratio of the positive charges of DOTAP to the negative charges of the RNA is 1.8:2 to 0.8:2, more preferably 1.6:2 to 1:2, even more preferably 1.4:2 to 1.1:2, even more preferably about 1.2:2.

[0082] In one embodiment, the nanoparticles are lipoplexes comprising a molar ratio of DOTMA and DOPE of 2:1 to 1:2, preferably 2:1 to 1:1, and the charge ratio of the positive charge of DOTMA to the negative charge of the RNA is 1.4:1 or less.

[0083] In one embodiment, the nanoparticles are lipoplexes comprising DOTMA and cholesterol in a molar ratio of 2:1 to 1:2, preferably 2:1 to 1:1, and the charge ratio of the positive charge of DOTMA to the negative charge of the RNA is 1.4:1 or less.

[0084] In one embodiment, the nanoparticles are lipoplexes comprising DOTAP and DOPE in a molar ratio of 2:1 to 1:2, preferably 2:1 to 1:1, and the charge ratio of the positive charge of DOTAP to the negative charge of RNA is 1.4:1 or less.

[0085] In accordance with the present invention, the term "F12" refers to liposomes containing DOTMA and DOPE in a molar ratio of 2:1 and lipoplexes containing RNA formed using such liposomes.

[0086] In accordance with the present invention, the term "F5" refers to liposomes containing DOTMA and cholesterol in a 1:1 molar ratio and lipoplexes containing RNA formed using such liposomes.

[0087] As used herein, the term "nanoparticle" refers to any particle having a diameter that makes the particle particularly suitable for systemic administration, particularly parenteral administration, of nucleic acids, typically less than 1000 nanometers (nm). In some embodiments, the nanoparticles have a diameter less than 600 nm. In some embodiments, the nanoparticles have a diameter less than 400 nm. In some embodiments, the nanoparticles have an average diameter ranging from about 50 nm to about 1000 nm, preferably from about 50 nm to about 400 nm, preferably from about 100 nm to about 300 nm, such as from about 150 nm to about 200 nm. In some embodiments, the nanoparticles have a diameter ranging from about 200 to about 700 nm, from about 200 to about 600 nm, preferably from about 250 to about 550 nm, particularly from about 300 to about 500 nm or from about 200 to about 400 nm.

[0088] As used herein, the term "nanoparticle formulation" or similar terms refers to any material that includes at least one nanoparticle. In some embodiments, the nanoparticle formulation is a homogenous collection of nanoparticles. In some embodiments, the nanoparticle formulation is a dispersion or emulsion. Generally, a dispersion or emulsion is formed when at least two immiscible materials are combined.

[0089] The terms "lipoplex" or "nucleic acid lipoplex", particularly "RNA lipoplex", refer to a complex of lipid and nucleic acid, particularly RNA. Lipoplexes form spontaneously when cationic liposomes, which often also contain neutral "helper" lipids, are mixed with nucleic acid.

[0090] When the present invention refers to a charge such as a positive charge, a negative charge or a neutral charge or a cationic, negative or neutral compound, this generally means that the charge referred to exists at a selected pH, such as physiological pH. For example, the term "cationic lipid" refers to a lipid that has a net positive charge at a selected pH, such as physiological pH. The term "neutral lipid" refers to a lipid that does not have a net positive or negative charge and can exist in the form of an uncharged or neutral zwitterion at a selected pH, such as physiological pH. "Physiological pH" herein refers to a pH of about 7.5.

[0091] Nanoparticle carriers such as lipid carriers contemplated for use in the present invention include any substance or vehicle that can associate with nucleic acid such as RNA, for example by forming a complex with the nucleic acid or by forming a vesicle in which the nucleic acid is enclosed or encapsulated. This may improve the stability of the nucleic acid compared to naked nucleic acid. In particular, it may improve the stability of the nucleic acid in blood.

[0092] Cationic lipids, cationic polymers, and other substances that carry a positive charge can form complexes with negatively charged nucleic acids. These cationic molecules can be used to complex nucleic acids, thereby forming, for example, so-called lipoplexes or polyplexes, respectively, and these complexes have been shown to deliver nucleic acids into cells.

[0093] The nanoparticle nucleic acid preparations used in the present invention can be obtained by various protocols and from various nucleic acid complexing compounds.Lipids, polymers, oligomers, or amphiphiles are typical complexing agents.In one embodiment, the complexing compound comprises at least one agent selected from the group consisting of protamine, polyethyleneimine, poly-L-lysine, poly-L-arginine, or histones.

[0094] According to the present invention, protamine is useful as a cationic carrier agent. The term "protamine" refers to any of a variety of relatively low molecular weight strongly basic proteins that are rich in arginine and have been found to be specifically associated with DNA in place of somatic histones in sperm cells of various animals (such as fish). In particular, the term "protamine" refers to a protein found in fish sperm that is strongly basic, soluble in water, does not coagulate by heat, and produces mainly arginine upon hydrolysis. In purified form, they are used to neutralize the anticoagulant effect of heparin in long-acting formulations of insulin. According to the present invention, the term "protamine" as used herein is meant to include any protamine amino acid sequence obtained or derived from natural or biological sources, including fragments thereof and multimeric forms of the amino acid sequence or fragments thereof. Furthermore, the term encompasses (synthesized) polypeptides that are artificial, specifically designed for a specific purpose, and cannot be isolated from natural or biological sources. The protamine used according to the present invention can be sulfated protamine or hydrochloride protamine. In a preferred embodiment, the protamine source used in the preparation of the nanoparticles described herein is Protamine 5000, which contains greater than 10 mg / ml (5000 heparin neutralizing units per ml) of protamine in an isotonic saline solution.

[0095] Liposomes are microscopic lipid vesicles, often with one or more bilayers of vesicle-forming lipids such as phospholipids, that can encapsulate drugs. Various types of liposomes can be used in the context of the present invention, including, but not limited to, multilamellar vesicles (MLVs), small unilamellar vesicles (SUVs), large unilamellar vesicles (LUVs), sterically stabilized liposomes (SSLs), multivesicular vesicles (MVs), and large multivesicular vesicles (LMVs), as well as other bilayer forms known in the art. The size and lamellar nature of the liposomes depend on the preparation method, and the choice of the vesicle type used depends on the preferred mode of administration. There are several other supramolecular organization forms in which lipids can exist in aqueous media, including lamellar phases, hexagonal and reverse hexagonal phases, cubic phases, micelles, reverse micelles consisting of a single layer. These phases can also be obtained in combination with DNA or RNA, and interactions with RNA and DNA can substantially affect the phase state. The phases described can be present in the nanoparticulate nucleic acid formulations of the present invention.

[0096] In forming a nucleic acid lipoplex from a nucleic acid and a liposome, any suitable method of forming a liposome can be used so long as it provides the intended nucleic acid lipoplex. The liposomes can be formed using standard methods such as reverse evaporation (REV), ethanol injection, dehydration-rehydration (DRV), sonication, or other suitable methods.

[0097] After liposome formation, the liposomes can be sized to obtain a population of liposomes having a substantially uniform size range.

[0098] Bilayer-forming lipids typically have two hydrocarbon chains, particularly acyl chains, and a polar or non-polar head group. Bilayer-forming lipids consist of naturally occurring lipids or are of synthetic origin, including phospholipids such as phosphatidylcholine, phosphatidylethanolamine, phosphatidic acid, phosphatidylinositol, and sphingomyelin, in which the two hydrocarbon chains are typically about 14-22 carbon atoms long and have different degrees of unsaturation. Other lipids suitable for use in the compositions of the invention include glycolipids and sterols, such as cholesterol, and various analogs thereof, which may also be used in liposomes.

[0099] Cationic lipids typically have a lipophilic moiety, such as a sterol, acyl or diacyl chain, and an overall net positive charge. The head group of the lipid typically carries a positive charge. Cationic lipids preferably have 1 to 10 positive charges, more preferably 1 to 3 positive charges, and more preferably a single positive charge. Examples of cationic lipids include, but are not limited to, 1,2-di-O-octadecenyl-3-trimethylammonium propane (DOTMA); dimethyldioctadecylammonium (DDAB); 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP); 1,2-dioleoyl-3-dimethylammonium-propane (DODAP); 1,2-diacyloxy-3-dimethylammonium propane; 1,2-dialkyloxy-3-dimethylammonium propane; dioctadecyldimethylammonium chloride (DODAC), 1,2-dimyristoyloxypropyl-1,3-dimethylhydroxyethylammonium (DMRIE), and 2,3-dioleoyloxy-N-[2(sperminecarboxamido)ethyl]-N,N-dimethyl-1-propanamide trifluoroacetate (DOSPA). Preferred are DOTMA, DOTAP, DODAC, and DOSPA. Most preferred is DOTMA.

[0100] In addition, the nanoparticles described herein preferably further contain a neutral lipid in terms of structural stability and the like. The neutral lipid can be appropriately selected in consideration of the delivery efficiency of the nucleic acid-lipid complex. Examples of neutral lipids include, but are not limited to, 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine (DOPE), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), diacylphosphatidylcholine, diacylphosphatidylethanolamine, ceramide, sphingomyelin, cephalin, sterol, and cerebroside. DOPE and / or DOPC are preferred. DOPE is most preferred. When the cationic liposome contains both a cationic lipid and a neutral lipid, the molar ratio of the cationic lipid to the neutral lipid can be appropriately determined in consideration of the stability of the liposome and the like.

[0101] According to one embodiment, the nanoparticles described herein may comprise phospholipids. The phospholipids may be glycerophospholipids. Examples of glycerophospholipids include, but are not limited to, three types of lipids: (i) zwitterionic phospholipids, such as phosphatidylcholine (PC), egg yolk phosphatidylcholine, soybean-derived PC in native, partially hydrogenated or fully hydrogenated form, dimyristoylphosphatidylcholine (DMPC) sphingomyelin (SM); (ii) negatively charged phospholipids, such as phosphatidylserine (PS), phosphatidylinositol (PI), phosphatidic acid (PA), phosphatidylglycerolipids (SM), and (iii) phospholipids that are soluble in water. (PG) dipalmipoyl PG, dimyristoyl phosphatidylglycerol (DMPG); synthetic derivatives in which the conjugate is negatively charged to a zwitterionic phospholipid, as in the case of methoxy-polyethylene glycol-distearoylphosphatidylethanolamine (mPEG-DSPE); and (iii) cationic phospholipids, e.g., phosphatidylcholine or sphingomyelin whose phosphomonoesters have been O-methylated to form cationic lipids.

[0102] Binding of nucleic acids to lipid carriers can occur, for example, by the nucleic acid filling the interstitial spaces of the carrier such that the carrier physically entraps the nucleic acid, or by adsorption through covalent, ionic, or hydrogen bonds, or non-specific binding.

[0103] The term "immune response" relates to a reaction of the immune system, preferably to an antigen, and preferably refers to a cellular immune response, a humoral immune response, or both. The immune response may be protective / preventive / prophylactic and / or therapeutic. According to the present invention, the term "immune response to" or "immune response against" in reference to a target such as an antigen, a cell or a tissue, relates to an immune response directed against the target.

[0104] "Stimulating an immune response" can mean that there was no immune response against a particular target, such as a target antigen, before stimulating the immune response, but it can also mean that there is a certain level of immune response against a particular target before stimulating the immune response, and that the immune response is enhanced after stimulating the immune response. Thus, "stimulating an immune response" includes "inducing an immune response" and "enhancing an immune response". Preferably, after stimulating the immune response of a subject, the subject is protected from developing a disease, such as a cancer disease, or the disease state is ameliorated by stimulating the immune response. For example, an immune response against a tumor antigen can be stimulated in a patient with a cancer disease or in a subject at risk of developing a cancer disease. Stimulating an immune response in this case can mean that the subject's disease state is ameliorated, that the subject does not develop metastasis, or that a subject at risk of developing a cancer disease does not develop a cancer disease.

[0105] The term "cellular immune response", "cellular response", "cell-mediated immunity" or similar terms are meant to include a cellular response to cells characterized by expression of antigens and / or presentation of antigens with class I or class II MHC. Cellular responses involve cells called T cells or T lymphocytes that act as "helpers" or "killers". Helper T cells (CD4+ T cells (also called CD8 T cells) play a central role in regulating the immune response and are involved in the production of killer cells (cytotoxic T cells, cytolytic T cells, and + T cells or CTLs) kill cells, such as diseased cells.

[0106] The term "humoral immune response" refers to the process in an organism whereby antibodies are produced in response to agents and organisms that are ultimately neutralized and / or eliminated. The specificity of the antibody response is mediated by T and / or B cells through membrane-bound receptors that bind to antigens of a single specificity. Following binding of the appropriate antigen and receipt of various other activation signals, B lymphocytes divide and generate memory B cells as well as antibody-secreting plasma cell clones, each of which produces antibodies that recognize the same antigenic epitope as that recognized by its antigen receptor. Memory B lymphocytes remain quiescent until subsequently activated by their specific antigen. These lymphocytes provide the cellular basis of memory and a resulting increase in antibody response when re-exposed to the specific antigen.

[0107] The term "antibody" as used herein refers to an immunoglobulin molecule capable of specifically binding to an epitope on an antigen. In particular, the term "antibody" refers to a glycoprotein comprising at least two heavy (H) chains and two light (L) chains interconnected by disulfide bonds. The term "antibody" includes monoclonal antibodies, recombinant antibodies, human antibodies, humanized antibodies, chimeric antibodies, and any combination of the above. Each heavy chain is composed of a heavy chain variable region (VH) and a heavy chain constant region (CH). Each light chain is composed of a light chain variable region (VL) and a light chain constant region (CL). The variable and constant regions are also referred to herein as variable and constant domains, respectively. The VH and VL regions can be further subdivided into hypervariable regions, called complementarity determining regions (CDRs), interspersed with more conserved regions, called framework regions (FRs). Each VH and VL is composed of three CDRs and four FRs arranged from amino terminus to carboxy terminus in the following order: FR1, CDR1, FR2, CDR2, FR3, CDR3, FR4. The CDRs of VH are called HCDR1, HCDR2, and HCDR3, and the CDRs of VL are called LCDR1, LCDR2, and LCDR3. The variable regions of the heavy and light chains contain binding domains that interact with antigens. The constant region of an antibody contains a heavy chain constant region (CH) and a light chain constant region (CL), and the CH can be further subdivided into a constant domain CH1, a hinge region, and constant domains CH2 and CH3 (arranged from amino terminus to carboxy terminus in the following order: CH1, CH2, CH3). The constant region of an antibody can mediate the binding of the immunoglobulin to host tissues or factors, including various cells of the immune system (e.g., effector cells) and the first component (Clq) of the classical complement system. An antibody can be an intact immunoglobulin derived from natural or recombinant sources, or an immunologically active portion of an intact immunoglobulin. An antibody is typically a tetramer of an immunoglobulin molecule. An antibody can exist in a variety of forms, including, for example, polyclonal antibodies, monoclonal antibodies, Fv, Fab and F(ab)2, as well as single chain antibodies and humanized antibodies.

[0108] Antibodies as described herein include IgA, e.g., IgA1 or IgA2, IgG1, IgG2, IgG3, IgG4, IgE, IgM, and IgD antibodies. In various embodiments, the antibody is an IgG1 antibody, more specifically an IgG1, kappa or IgG1, lambda isotype (i.e., IgG1, κ, λ), an IgG2a antibody (e.g., IgG2a, κ, λ), an IgG2b antibody (e.g., IgG2b, κ, λ), an IgG3 antibody (e.g., IgG3, κ, λ), or an IgG4 antibody (e.g., IgG4, κ, λ).

[0109] The term "immunoglobulin" relates to proteins of the immunoglobulin superfamily, preferably antigen receptors, such as antibodies or B cell receptors (BCR). Immunoglobulins are characterized by structural domains, i.e. immunoglobulin domains, with a characteristic immunoglobulin (Ig) fold. The term includes membrane-bound immunoglobulins as well as soluble immunoglobulins. Membrane-bound immunoglobulins, also called surface or membrane immunoglobulins, are generally part of the BCR. Soluble immunoglobulins are generally called antibodies. Immunoglobulins generally comprise several chains, typically two identical heavy chains and two identical light chains linked via disulfide bonds. These chains are classified into V L (Variable light chain) domain, C L (constant light chain) domain, V H (variable heavy chain) domain, and C H (Constant Heavy Chain) Domain C H 1. C H 2. C H 3, and C H4. There are five types of immunoglobulin heavy chains in mammals, namely α, δ, ε, γ, and μ, which constitute the different classes of antibodies, namely IgA, IgD, IgE, IgG, and IgM. In contrast to the heavy chains of soluble immunoglobulins, the heavy chains of membrane or surface immunoglobulins contain a transmembrane domain and a short cytoplasmic domain at their carboxy termini. There are two types of light chains in mammals, namely lambda and kappa. Immunoglobulin chains contain a variable region and a constant region. The constant region is essentially conserved within the different isotypes of immunoglobulins, while the variable part is highly diverse and is the main responsible for antigen recognition.

[0110] According to the present invention, the term "antigen" or "immunogen" encompasses any substance, preferably a peptide or protein, which is the target of and / or induces an immune response. In particular, "antigen" relates to any substance which reacts specifically with antibodies or T lymphocytes (T cells). According to the present invention, the term "antigen" includes any molecule comprising at least one epitope, such as a B-cell or T-cell epitope, suitable for vaccination. Preferably, an antigen in the context of the present invention is a molecule which, optionally after processing, induces an immune response, preferably specific to the antigen or to a cell expressing the antigen. According to the present invention, any suitable antigen may be used which is a candidate for an immune response. The antigen is preferably a product which corresponds to or is derived from a naturally occurring antigen. Such naturally occurring antigens may include or be derived from allergens, viruses, bacteria, fungi, parasites and other infectious agents and pathogens, or the antigen may also be a tumor antigen. In a preferred embodiment, the antigen is or is derived from a surface polypeptide, i.e., a polypeptide that is naturally displayed on the surface of a cell, a pathogen, a bacterium, a virus, a fungus, a parasite, an allergen, or a tumor. The antigen is capable of eliciting an immune response against the cell, pathogen, bacterium, virus, fungus, parasite, allergen, or tumor.

[0111] According to the invention, the antigen may be selected from the group comprising self and non-self antigens, such as bacterial antigens, viral antigens, fungal antigens, allergens or parasitic antigens.

[0112] In a preferred embodiment, the antigen is associated with a disease or disorder, i.e., the antigen is a disease-associated antigen. The term "disease-associated antigen" refers to any antigen of pathological importance. In a particularly preferred embodiment, the disease-associated antigen is present in diseased cells, tissues and / or organs, but absent or present in reduced amounts in healthy cells, tissues and / or organs, and can therefore be used to target diseased cells, tissues and / or organs. In one embodiment, the disease-associated antigen is present on the surface of a diseased cell. In one embodiment, the disease-associated antigen is a molecule that comprises at least one epitope that stimulates the host's immune system to elicit a humoral and / or cellular immune response against the disease. Thus, the disease-associated antigen can be used for therapeutic purposes. The disease-associated antigen is preferably associated with infection by a microorganism, typically a microbial antigen, or associated with cancer, typically a tumor.

[0113] In some embodiments, the antigen is a bacterial antigen or is derived from a bacterial antigen. In some embodiments, the antigen elicits an immune response against a bacterium that infects animals, including birds, fish, and mammals, including domesticated animals. Preferably, the bacterium against which an immune response is elicited is a pathogenic bacterium.

[0114] In some embodiments, the antigen is or is derived from a viral antigen. A viral antigen can be, for example, a peptide derived from a viral surface protein, such as a capsid polypeptide or a spike polypeptide. In some embodiments, the antigen elicits an immune response against a virus that infects animals, including birds, fish, and mammals, including domesticated animals. Preferably, the virus against which an immune response is elicited is a pathogenic virus.

[0115] In some embodiments, the antigen is or is derived from a peptide or protein derived from a fungus. In some embodiments, the antigen elicits an immune response against a fungus that infects animals, including birds, fish, and mammals, including domesticated animals. Preferably, the fungus against which an immune response is elicited is a pathogenic fungus.

[0116] In some embodiments, the antigen is or is derived from a peptide or protein from a unicellular eukaryotic parasite. In some embodiments, the antigen induces an immune response against a unicellular eukaryotic parasite, preferably a pathogenic unicellular eukaryotic parasite. The pathogenic unicellular eukaryotic parasite can be, for example, from the genus Plasmodium, such as P. falciparum, P. vivax, P. malariae, or P. ovale, the genus Leishmania, or the genus Trypanosoma, such as T. cruzi or T. brucei.

[0117] In some embodiments, the antigen is or is derived from an allergenic peptide or protein. The allergenic peptide or protein is suitable for allergen immunotherapy, also known as hyposensitization.

[0118] In a preferred embodiment, the antigen is a tumor antigen or a tumor-associated antigen, i.e. an antigen that is produced, preferably in large amounts, as a component of cancer cells, which may originate from the cytoplasm, the cell surface and the cell nucleus, in particular as a surface antigen on cancer cells.

[0119] In the context of the present invention, the term "tumor antigen" or "tumor associated antigen" relates to a protein that is specifically expressed in a limited number of tissues and / or organs under normal conditions or at a particular development stage, for example a tumor antigen may be specifically expressed under normal conditions in gastric tissue, preferably gastric mucosa, reproductive organs, such as testes, trophoblastic tissue, such as placenta, or germ line cells, and is expressed or aberrantly expressed in one or more tumor or cancer tissues. In this context, "limited number" preferably means 3 or less, more preferably 2 or less. Tumor antigens in the context of the present invention include, for example, differentiation antigens, preferably cell type specific differentiation antigens, i.e. proteins that are specifically expressed under normal conditions in a given cell type at a given differentiation stage, cancer / testis antigens, i.e. proteins that are specifically expressed under normal conditions in the testis and sometimes in the placenta, and germ line specific antigens. In the context of the present invention, tumor antigens are preferably associated with the cell surface of cancer cells and are preferably not or only rarely expressed in normal tissues. Preferably, the tumor antigen or the aberrant expression of the tumor antigen identifies the cancer cells. In the context of the present invention, the tumor antigen expressed by cancer cells in a subject, for example a patient suffering from a cancer disease, is preferably a self-protein of said subject.In a preferred embodiment, the tumor antigen in the context of the present invention is expressed under normal conditions, particularly in non-essential tissues or organs, i.e. in tissues or organs that do not lead to the death of the subject when damaged by the immune system, or in organs or structures of the body that are inaccessible or poorly accessible to the immune system.Preferably, the amino acid sequence of the tumor antigen is identical between the tumor antigen expressed in normal tissues and the tumor antigen expressed in cancer tissues.

[0120] Examples of tumor antigens that may be useful in the present invention are p53, ART-4, BAGE, β-catenin / m, Bcr-abL CAMEL, CAP-1, CASP-8, CDC27 / m, CDK4 / m, CEA, cell surface proteins of the claudin family, such as CLAUDIN-6, CLAUDIN-18.2 and CLAUDIN-12, c-MYC, CT, Cyp-B, DAM, ELF2M, ETV6-AML1, G250, GAGE, GnT-V, Gap100, HAGE, HER-2 / neu, HPV-E7, HPV-E6, HAST-2, hTERT (or hTRT), LAGE, LDLR / FUT, MAGE-A, preferably MAGE-A1, MAGE-A2, MAGE-A3, MAGE-A4, MAGE-A5, MAGE-A6, MAGE-A7, MAGE-A8, MAGE-A9, MAGE-A10, MAGE-A11, MAGE-A12, MAGE-A13, MAGE-A14, MAGE-A15, MAGE-A16, MAGE-A17, MAGE-A18, MAGE-A19, MAGE-A20, MAGE-A21, MAGE-A22, MAGE-A23, MAGE-A24, MAGE-A25, MAGE-A26, MAGE-A27, MAGE-A28, MAGE-A29, MAGE-A30, MAGE-A31, MAGE-A32, MAGE-A33, MAGE-A34, MAGE-A35, MAGE-A36, MAGE-A37, MAGE-A38, MAGE-A39, MAGE-A39, MAGE-A31, MAGE-A32, MAGE-A33, MAGE-A34, MAGE-A35 -A8, MAGE-A9, MAGE-A10, MAGE-A11 or MAGE-A12, MAGE-B, MAGE-C, MART-1 / Melan-A, MC1R, Myosin / m, MUC1, MUM-1, -2, -3, NA88-A, NF1, NY-ESO-1, NY-BR-1, p190 minor BCR-abL, Pm1 / RARa, PRAME, proteinase 3, PSA, PSM, RAGE, RU1 or RU2, SAGE, SART-1 or SART-3, SCGB3A2, SCP1, SCP2, SCP3, SSX, SURVIVIN, TEL / AML1, TPI / m, TRP-1, TRP-2, TRP-2 / INT2, TPTE and WT. Particularly preferred tumor antigens include CLAUDIN-18.2 (CLDN18.2) and CLAUDIN-6 (CLDN6).

[0121] The antigens provided to the subject according to the present invention, i.e. vaccine antigens, by administering the antigen or a nucleic acid encoding the antigen, should result in a B-cell and / or T-cell response. The antibodies and / or T-cells should be directed against the target antigen, in particular the target antigen expressed by or in the diseased cells, tissues and / or organs, i.e. the disease-associated antigen. Thus, the vaccine antigen may correspond to or comprise the disease-associated antigen or may be a variant thereof. In one embodiment, such variants are immunologically equivalent to the disease-associated antigen. In the context of the present invention, the term "variant of an antigen" refers to an agent that results in a B-cell and / or T-cell response that targets the antigen, i.e. the disease-associated antigen, especially when expressed in diseased cells, tissues and / or organs, or in cells that express the antigen and optionally present the antigen in the context of MHC molecules. Thus, the vaccine antigen may be identical to the disease-associated antigen, may comprise the disease-associated antigen or a part thereof, or may comprise an antigen that is homologous to the disease-associated antigen or a part thereof. When the vaccine antigen comprises a part of a disease-associated antigen or a part of an antigen homologous to a disease-associated antigen, said part may comprise an epitope of the disease-associated antigen against which a B-cell response and / or T-cells are targeted. Thus, according to the present invention, the antigen may comprise an immunogenic fragment of the disease-associated antigen, such as a peptide fragment of the disease-associated antigen. An "immunogenic fragment of an antigen" according to the present invention preferably relates to a part or fragment of the antigen that is capable of stimulating a B-cell response and / or a T-cell response. The vaccine antigen or the nucleic acid encoding the vaccine antigen administered according to the present invention may be a recombinant antigen or a recombinant nucleic acid.

[0122] The term "immunologically equivalent" means that immunologically equivalent molecules, such as immunologically equivalent amino acid sequences, exhibit the same or essentially the same immunological properties and / or exert the same or essentially the same immunological effect, e.g., with respect to the type of immunological effect. In the context of the present invention, the term "immunologically equivalent" is preferably used with respect to the immunological effect or properties of the antigen or antigen variant used for immunization. For example, an amino acid sequence is immunologically equivalent to a reference amino acid sequence if, when exposed to the immune system of a subject, the amino acid sequence induces an immune response with the specificity to react with the reference amino acid sequence.

[0123] The term "epitope" refers to an antigenic determinant in a molecule such as an antigen, i.e., a part or fragment of a molecule that is recognized and thus bound by the immune system, e.g., recognized by an antibody or a T-cell receptor. For example, an epitope is a discrete three-dimensional site on an antigen that is recognized by the immune system. Epitopes are usually composed of chemically active surface molecular groupings, such as amino acids or sugar side chains, and usually have specific three-dimensional structural characteristics and specific charge characteristics. Conformational and non-conformational epitopes are distinguished in that binding to the former, but not the latter, is lost in the presence of denaturing solvents. Preferably, an epitope is capable of eliciting an immune response against the antigen or a cell expressing the antigen. Preferably, the term relates to an immunogenic portion of an antigen that contains the epitope. An epitope of a protein, such as a tumor antigen, preferably comprises a continuous or discontinuous portion of said protein and is preferably 5 to 100, preferably 5 to 50, more preferably 8 to 30, most preferably 10 to 25 amino acids in length, for example an epitope may preferably be 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 or 25 amino acids in length. Preferably, an epitope in the context of the present invention is a B cell epitope or a T cell epitope.

[0124] As used herein, the term "T cell epitope" refers to a peptide that binds to an MHC molecule in a conformation recognized by a T cell receptor. Typically, T cell epitopes are presented on the surface of antigen presenting cells. A "T cell epitope" according to the present invention preferably relates to a part or fragment of an antigen capable of stimulating an immune response, preferably a cell characterized by expression of the antigen, preferably presentation of the antigen, or a cellular response against the antigen. Preferably, the T cell epitope is capable of stimulating a cellular response against a cell characterized by presentation of the antigen. Preferably, the T cell epitope is an MHC class I and / or class II presented peptide. Preferably, the T cell epitope comprises an amino acid sequence that substantially corresponds to the amino acid sequence of a fragment of the antigen. Preferably, said fragment of the antigen is an MHC class I and / or class II presented peptide. Peptides suitable for binding to MHC molecules, in particular class I MHC molecules, are preferably 7 to 20 amino acids in length, more preferably 7 to 12 amino acids in length, more preferably 8 to 11 amino acids in length, in particular 9 or 10 amino acids in length. In one embodiment, T cell epitopes, when presented in the context of an MHC, such as the MHC of an antigen presenting cell, are recognized by a T cell receptor. When recognized by a T cell receptor, the T cell epitope may be capable of inducing, in the presence of an appropriate costimulatory signal, the clonal expansion of T cells carrying a T cell receptor that specifically recognizes the T cell epitope. Preferably, T cell epitopes, especially when presented in the context of an MHC molecule, are capable of stimulating an immune response, preferably a cellular response, against the antigen from which they are derived or against cells characterized by expression of the antigen, preferably characterized by presentation of the antigen.

[0125] According to the present invention, T cell epitopes may be present in a vaccine antigen as part of a larger entity such as a vaccine sequence and / or a polypeptide comprising more than one T cell epitope. The presented peptide or T cell epitope is produced after appropriate processing. Also, T cell epitopes may be modified at one or more residues that are not essential for TCR recognition or binding to MHC. Such modified T cell epitopes may be considered to be immunologically equivalent.

[0126] Vaccination according to the invention using the antigens described herein preferably results in an immune response against a disease-associated antigen or epitope thereof. Preferably, such disease-associated antigen or epitope thereof comprises one or more disease-specific amino acid modifications, e.g. they comprise or are disease-associated neo-antigens or neo-epitopes. Preferably, the disease-specific amino acid modifications are due to one or more disease-specific somatic mutations. In a particularly preferred embodiment, the disease-specific amino acid modifications are cancer-specific amino acid modifications and the disease-specific somatic mutations are cancer-specific somatic mutations. Thus, in one embodiment, the vaccine antigen is preferably characterized by the disease-specific amino acid modifications / disease-specific somatic mutations of the patient and preferably provides a neo-epitope based on one or more mutations upon administration. Thus, the vaccine antigen may comprise a peptide or polypeptide comprising a neo-epitope based on one or more mutations. In one embodiment, the disease-specific amino acid modifications are identified by identifying the disease-specific somatic mutations, e.g. by sequencing genomic DNA and / or RNA of the diseased tissue or one or more diseased cells.

[0127] As used herein, the term "neoepitope" refers to an epitope that is not present in a reference, such as a normal, non-diseased (e.g., non-cancerous) or germline cell, but is found in a diseased cell (e.g., a cancer cell). This specifically includes situations where a corresponding epitope is found in a normal, non-diseased or germline cell, but one or more mutations in the diseased cell have altered the sequence of the epitope to create a neoepitope.

[0128] According to the present invention, the term "vaccine" relates to a pharmaceutical preparation (pharmaceutical composition) or product that upon administration induces an immune response that recognizes and attacks a pathogen or diseased cells, such as cancer cells. Vaccines can be used for the prevention or treatment of a disease. In particular, the term "vaccine" refers to a composition comprising an antigen, as defined herein.

[0129] In one embodiment, the vaccines provided by the present invention comprise a nucleic acid, preferably RNA, encoding a vaccine antigen, also referred to herein simply as an "antigen", or a peptide or protein antigen, as described herein, for stimulating a therapeutically or prophylactically useful immune response.

[0130] The antigens described herein, when administered to a subject, preferably provide one or more epitopes suitable for stimulating a disease-specific immune response. In one embodiment, the disease-specific immune response is preferably an antigen-specific immune response against a disease-associated antigen. Presentation of these epitopes, for example by diseased cells or pathogens, serves as a label for targeting by the immune response.

[0131] In one embodiment of the invention, the antigens described herein are or are provided in the form of virus-like particles (VLPs). Virus-like particles resemble viruses but do not contain viral genetic material and are therefore non-infectious. Expression of viral structural proteins such as envelope and capsid may lead to self-assembly of virus-like particles. Virus-like particles have been produced from components of a wide variety of virus families including Parvoviridae (e.g., Adeno-associated virus), Retroviridae (e.g., HIV), Flaviviridae (e.g., Hepatitis C virus) and bacteriophages (e.g., Qβ, AP205). Virus-like particles can be produced in multiple cell culture systems including bacteria, mammalian cell lines, insect cell lines, yeast and plant cells. Virus-like particles are useful as vaccines. Virus-like particles contain a repetitive, high-density display of viral surface proteins that present conformational viral epitopes that can elicit strong T-cell and B-cell immune responses. Virus-like particles cannot replicate and therefore offer a safer alternative to attenuated viruses.

[0132] Virus-like particles, such as those derived from the Hepatitis B virus core antigen (HBcAg), are also useful as non-infectious carriers of foreign immune epitopes. The Hepatitis B virus core antigen (HBcAg) naturally assembles into particulate icosahedral nucleocapsids. Virus-like particles, such as recombinant HBcAg particles, can be used to display viral protein epitopes, bacterial and protozoan protein epitopes, and tumor antigen epitopes. The highly repetitive, dense display and spacing of the inserted epitopes appears to be optimal for cross-linking of B cell receptors.

[0133] Immunotherapeutic approaches according to the invention include immunization with peptide or protein antigens (natural or modified), nucleic acids encoding the peptide or protein antigens, recombinant cells encoding the peptide or protein antigens, recombinant viruses encoding the peptide or protein antigens, and antigen-presenting cells pulsed with the peptide or protein antigen (natural or modified) or transfected with nucleic acids encoding the peptide or protein antigens.

[0134] In one embodiment, the objective is to provide an immune response against cancer cells expressing tumor antigens and to treat cancer diseases involving cells expressing tumor antigens. The cancer cells expressing tumor antigens may express the tumor antigen on the surface of the cancer cells and / or may present the tumor antigen on the cell surface in the context of MHC molecules. Cancer cells expressing tumor antigens on the cell surface may be targeted by antibodies directed against the tumor antigen, in particular the extracellular portion of the tumor antigen. Cancer cells presenting tumor antigens on the cell surface in the context of MHC molecules may be targeted by T cells directed against T cell epitopes of the tumor antigen.

[0135] "Cell surface" is used according to its normal meaning in the art and thus includes the outside of a cell accessible to binding by proteins and other molecules. An antigen is expressed on the surface of a cell if it is located on the surface of the cell and is accessible to binding, for example, by an antigen-specific antibody added to the cell. In one embodiment, an antigen expressed on the surface of a cell is an integral membrane protein having an extracellular portion.

[0136] The term "extracellular portion" or "exodomain" in the context of the present invention refers to a part of a molecule, such as a protein, which faces the extracellular space of a cell and is preferably accessible from the outside of said cell, for example by binding to a molecule, such as an antibody, that is extracellular. Preferably, the term refers to one or more extracellular loops or domains or fragments thereof.

[0137] The terms "portion" or "part" are used interchangeably herein and refer to a continuous or discontinuous element of a structure, such as an amino acid sequence. The term "fragment" refers to a continuous element of a structure, such as an amino acid sequence. A part, portion or fragment of a structure preferably comprises one or more functional properties of said structure, such as antigenic, immunological and / or binding properties. A part or portion of a protein sequence preferably comprises at least 6, in particular at least 8, at least 12, at least 15, at least 20, at least 30, at least 50 or at least 100 consecutive and / or non-consecutive amino acids of the protein sequence. A fragment of a protein sequence preferably comprises at least 6, in particular at least 8, at least 12, at least 15, at least 20, at least 30, at least 50 or at least 100 consecutive amino acids of the protein sequence.

[0138] The term "immunogenicity" relates to the relative effectiveness of an antigen to induce an immune response.

[0139] The term "immunostimulatory" is used herein to refer to an increase in the overall immune response.

[0140] The term "target" is intended to mean a cell that is the target of an immune response, in particular an agent such as a cancer cell. Targets include cells that present an antigen or an antigen epitope, i.e. a peptide fragment derived from an antigen. In one embodiment, the target cell is a cell that expresses the target antigen, preferably present on the cell surface.

[0141] "Antigen processing" refers to the degradation of an antigen into processing products that are fragments of the antigen (e.g., degradation of a protein into peptides) and the association (e.g., by binding) of one or more of these fragments with an MHC molecule for presentation by a cell, preferably an antigen-presenting cell, to a specific T cell.

[0142] An antigen-presenting cell (APC) is a cell that displays antigens in the context of major histocompatibility complex (MHC) on its surface. T cells can recognize this complex using their T cell receptor (TCR). Antigen-presenting cells process antigens and present them to T cells. Antigen-presenting cells include, but are not limited to, monocytes / macrophages, B cells and dendritic cells (DCs). According to the present invention, the term "antigen-presenting cells" includes professional and non-professional antigen-presenting cells.

[0143] Professional antigen-presenting cells are highly efficient at internalizing antigens, either by phagocytosis or receptor-mediated endocytosis, and then displaying fragments of the antigen bound to class II MHC molecules on their membrane. T cells recognize and interact with the antigen-class II MHC molecule complex on the membrane of the antigen-presenting cell. Additional costimulatory signals are then generated by the antigen-presenting cell, leading to T cell activation. The expression of costimulatory molecules is the defining feature of professional antigen-presenting cells.

[0144] The main type of professional antigen-presenting cell is the dendritic cell, which has the broadest range of antigen presentation and is probably the most important antigen-presenting cell, macrophages, B cells, and certain activated epithelial cells.

[0145] Non-professional antigen-presenting cells do not constitutively express the MHC class II proteins required for interaction with naive T cells; these are expressed only upon stimulation of the non-professional antigen-presenting cells with certain cytokines, such as IFNγ.

[0146] Dendritic cells (DCs) are a population of leukocytes that present antigens captured in peripheral tissues to T cells via both MHC class II and I antigen presentation pathways. It is well known that dendritic cells are potent inducers of immune responses and that activation of these cells is a critical step in immune induction.

[0147] Dendritic cells are conveniently classified as "immature" and "mature" cells, which can be used as a simple method to distinguish between two well-characterized phenotypes, but this nomenclature should not be construed to exclude all possible intermediate stages of differentiation.

[0148] Immature dendritic cells are characterized as antigen-presenting cells with a high capacity for antigen uptake and processing, which correlates with high expression of Fcγ receptors and mannose receptors. The mature phenotype is typically characterized by low expression of these markers, but high expression of cell surface molecules responsible for T cell activation, such as class I and class II MHC, adhesion molecules (e.g., CD54 and CD11), and costimulatory molecules (e.g., CD40, CD80, CD86, and 4-1BB).

[0149] Dendritic cell maturation is referred to as a state of dendritic cell activation in which such antigen-presenting dendritic cells lead to T cell priming, whereas presentation by immature dendritic cells leads to tolerance. Dendritic cell maturation is mainly triggered by biomolecules with microbial characteristics detected by natural receptors (bacterial DNA, viral RNA, endotoxins, etc.), proinflammatory cytokines (TNF, IL-1, IFN), ligation of CD40 on the dendritic cell surface by CD40L, and substances released from cells undergoing stressful cell death. Dendritic cells can be induced by culturing bone marrow cells in vitro with cytokines such as granulocyte-macrophage colony-stimulating factor (GM-CSF) and tumor necrosis factor alpha.

[0150] The term "plasmacytoid dendritic cells" or "pDCs" refers to innate immune cells that circulate in the blood and are found in peripheral lymphoid organs. They arise from bone marrow hematopoietic stem cells and constitute <0.4% of peripheral blood mononuclear cells (PBMCs). In humans, plasmacytoid dendritic cells exhibit plasma cell morphology and express CD4, HLA-DR, CD123, blood-derived dendritic cell antigen-2 (BDCA-2), Toll-like receptor (TLR) 7 and TLR9 in endosomal compartments, but do not express high levels of CD11c or CD14, which distinguish them from common dendritic cells or monocytes, respectively. As components of the innate immune system, plasmacytoid dendritic cells express intracellular Toll-like receptors 7 and 9 that detect ssRNA and unmethylated CpG DNA sequences, respectively. Upon stimulation and subsequent activation, these cells produce large amounts (up to 1000-fold more than other cell types) of type I interferons (mainly IFN-α (alpha) and IFN-β (beta)).

[0151] By "cells characterized by antigen presentation" or "cells presenting antigen" or similar expressions is meant cells, such as diseased cells, e.g. cancer cells, or antigen-presenting cells, that present an antigen or a fragment derived from said antigen, e.g. by processing of the antigen in the context of an MHC molecule, particularly an MHC class I molecule. Similarly, the term "diseases characterized by antigen presentation" refers to diseases involving cells characterized by antigen presentation, particularly with class I MHC.

[0152] The term "immunoreactive cells" or "effector cells" in the context of the present invention relates to cells that exert effector functions during an immune response. "Immunoreactive cells" are preferably characterized by expression and / or presentation of antigens or epitopes and are capable of binding to cells or antigens that mediate the immune response. For example, such cells secrete cytokines and / or chemokines, kill microorganisms, secrete antibodies, recognize infected or cancerous cells and, in some cases, eliminate such cells. For example, immunoreactive cells include T cells (cytotoxic T cells, helper T cells, tumor-infiltrating T cells), B cells, natural killer cells, neutrophils, macrophages, and dendritic cells.

[0153] Preferably, an "immunoreactive cell" recognises an antigen or epitope with some degree of specificity, particularly when presented in the context of an MHC molecule, such as on the surface of an antigen-presenting cell or a diseased cell, such as a tumor cell. Preferably, said recognition enables the cell which recognises the antigen or epitope to become responsive or reactive. The cell may be a helper T cell (CD4 T cell) bearing a receptor which recognises the antigen or epitope in the context of an MHC class II molecule. + In the case of CD4+ T cells, such responsiveness or reactivity may be determined by cytokine release and / or CD8 +The activation of lymphocytes (CTLs) and / or B cells may include activation of CTLs. If the cell is a CTL, such responsiveness or reactivity may include elimination of cells presented in the context of MHC class I molecules, i.e., cells characterized by presentation of antigen by class I MHC, e.g., via apoptosis or perforin-mediated cytolysis. According to the present invention, CTL responsiveness may include sustained calcium flux, cell division, production of cytokines such as IFN-γ and TNF-α, upregulation of activation markers such as CD44 and CD69, and specific cytolytic killing of antigen-expressing target cells. CTL responsiveness may also be determined using artificial reporters that precisely indicate CTL responsiveness. Such CTLs that recognize and are responsive or reactive to an antigen or epitope are also referred to herein as "antigen-responsive CTLs". If the cell is a B cell, such responsiveness may include release of immunoglobulins.

[0154] The term "T cell" or "T lymphocyte" refers to thymus-derived cells that are involved in a variety of cell-mediated immune responses and include T helper cells (CD4+ T cells) and cytotoxic T cells (CTL, CD8+ T cells), including cytolytic T cells.

[0155] T cells belong to a group of white blood cells known as lymphocytes and play a central role in cell-mediated immunity. They can be distinguished from other lymphocytes, such as B cells and natural killer cells, by the presence of a special receptor on the cell surface called the T cell receptor (TCR). The thymus is the main organ responsible for the maturation of T cells. Several different subsets of T cells have been discovered, each with distinct functions.

[0156] T helper cells assist other white blood cells in immune processes, including, among other functions, maturation of B cells into plasma cells, activation of cytotoxic T cells and macrophages. These cells are also known as CD4+ T cells because they express the CD4 protein on their surface. Helper T cells become activated when they are presented with peptide antigens by MHC class II molecules expressed on the surface of antigen-presenting cells (APCs). Once activated, they divide rapidly and secrete small proteins called cytokines that regulate or support active immune responses.

[0157] Cytotoxic T cells destroy virus-infected and tumor cells and are also involved in transplant rejection. These cells are also known as CD8+ T cells because they express the CD8 glycoprotein on their surface. These cells recognize their targets by binding to antigens associated with MHC class I, which are present on the surface of almost every cell in the body.

[0158] The majority of T cells have a T cell receptor (TCR) that exists as a complex of several proteins. The actual T cell receptor is generated from independent T cell receptor alpha and beta (TCRα and TCRβ) genes and is composed of two separate peptide chains called α- and β-TCR chains. γδ T cells (gamma delta T cells) represent a small subset of T cells that have a separate T cell receptor (TCR) on their surface. However, in γδ T cells, the TCR is composed of one γ chain and one δ chain. This group of T cells is less common than αβ T cells (2% of all T cells).

[0159] The structure of the T cell receptor is very similar to the immunoglobulin Fab fragment, the region defined as the combination of the light and heavy chains of an antibody arm. Each chain of the TCR is a member of the immunoglobulin superfamily and has one N-terminal immunoglobulin (Ig) variable (V) domain, one Ig constant (C) domain, a transmembrane / cell-spanning region, and a short C-terminal cytoplasmic tail. The variable domains of both the TCR α and β chains contain three hypervariable or complementarity determining regions (CDRs), but the variable region of the β chain has an additional hypervariable region (HV4) that does not normally contact antigen and is therefore not considered a CDR. CDR1 of the α chain has also been shown to interact with the N-terminal portion of antigenic peptides, while CDR3 is the main CDR involved in recognition of processed antigens, and CDR1 of the β chain interacts with the C-terminal portion of the peptide. CDR2 is believed to recognize MHC. CDR4 of the β chain is not believed to be involved in antigen recognition, but has been shown to interact with superantigens. The constant domain of the TCR domain consists of a short linking sequence in which cysteine ​​residues form disulfide bonds, forming the link between the two chains.

[0160] The term "B cell" or "B lymphocyte" refers to a type of white blood cell of lymphocyte subtype that functions in humoral immunity by secreting antibodies. In addition, B cells present antigens, are classified as professional antigen-presenting cells (APC), and secrete cytokines. B cells express the B cell receptor (BCR) on their cell membrane. The BCR allows B cells to bind to specific antigens and initiate an antibody response against them. The B cell receptor is composed of two parts: a membrane-bound immunoglobulin molecule of one isotype (IgD, IgM, IgA, IgG, or IgE), which is identical to its secreted form except for the presence of an integral membrane domain, and a signaling part: a heterodimer called Ig-α / Ig-β (CD79) linked by a disulfide bridge. Each member of the dimer spans the plasma membrane and has a cytoplasmic tail that bears an immunoreceptor tyrosine-based activation motif (ITAM).

[0161] B cell activation occurs in secondary lymphoid organs such as the spleen and lymph nodes. After maturation in the bone marrow, B cells migrate via the blood to secondary lymphoid organs where they receive a constant supply of antigens through circulating lymph. B cell activation begins when a B cell binds to an antigen via its BCR. Different B cell subsets undergo T cell-dependent or T cell-independent activation.

[0162] The term "peripheral blood mononuclear cells" or "PBMCs" refers to peripheral blood cells with a round nucleus. These cells consist of lymphocytes (T cells, B cells, NK cells) and monocytes, whereas red blood cells and platelets lack a nucleus and granulocytes (neutrophils, basophils, eosinophils) have a multilobed nucleus. These cells can be extracted from whole blood using Ficoll and gradient centrifugation, which separates the blood into a top layer of plasma, followed by a layer of PBMCs and a bottom fraction of polymorphonuclear cells (such as neutrophils and eosinophils) and red blood cells.

[0163] The terms "major histocompatibility complex" and the abbreviation "MHC" refer to a complex of genes that includes MHC class I and MHC class II molecules and occurs in all vertebrates. MHC proteins or molecules are important in signaling between lymphocytes and antigen-presenting or diseased cells in an immune response; MHC proteins or molecules bind peptides and present them for recognition by T cell receptors. Proteins encoded by MHC are expressed on the surface of cells and display both self antigens (peptide fragments from the cell itself) and non-self antigens (e.g., fragments of invading microorganisms) to T cells.

[0164] MHC regions are divided into three subgroups: class I, class II, and class III. MHC class I proteins contain α chains and β2-microglobulin (not part of the MHC encoded on chromosome 15). They present antigen fragments to cytotoxic T cells. In most immune system cells, especially antigen-presenting cells, MHC class II proteins contain α chains and β chains, which present antigen fragments to T helper cells. MHC class III regions code for other immune components, such as complement components and some that code for cytokines.

[0165] In humans, the genes of the MHC region that code for antigen-presenting proteins on the cell surface are called human leukocyte antigen (HLA) genes. However, the abbreviation MHC is often used to refer to the HLA gene products. The HLA genes include nine so-called classical MHC genes: HLA-A, HLA-B, HLA-C, HLA-DPA1, HLA-DPB1, HLA-DQA1, HLA-DQB1, HLA-DRA, and HLA-DRB1.

[0166] In a preferred embodiment of all aspects of the invention, the MHC molecule is an HLA molecule.

[0167] The term "immune effector function" or "effector function" in the context of the present invention includes any function mediated by a component of the immune system that results in, for example, the killing of a cell. Preferably, the immune effector function in the context of the present invention is a T cell-mediated effector function. Such a function can be mediated by helper T cells (CD4 + In the case of T cells, recognition of an antigen or an antigen-derived peptide in the context of an MHC class II molecule by a T cell receptor, release of cytokines, and / or CD8 + It involves the activation of lymphocytes (CTLs) and / or B cells and, in the case of CTLs, the recognition of antigens or antigenic peptides derived from antigens in the context of MHC class I molecules by T cell receptors, and the elimination of cells presented in the context of MHC class I molecules, i.e., cells characterized by presentation of the antigen with class I MHC, e.g., by apoptosis or perforin-mediated cell lysis, production of cytokines such as IFN-γ and TNF-α, and specific cytolytic killing of antigen-expressing target cells.

[0168] "Antibody-dependent cell-mediated cytotoxicity" or "ADCC" describes the cell killing ability of effector cells, particularly lymphocytes, as described herein, which preferably requires that target cells are labeled with an antibody. ADCC preferably occurs when an antibody binds to an antigen on a tumor cell and the antibody Fc domain binds to an Fc receptor (FcR) on the surface of an immune effector cell. Several families of Fc receptors have been identified, and certain cell populations characteristically express defined Fc receptors. ADCC can be viewed as a mechanism to directly induce various degrees of immediate tumor destruction leading to antigen presentation and induction of tumor-directed T cell responses. Preferably, in vivo induction of ADCC will result in tumor-directed T cell responses and host-derived antibody responses.

[0169] "Complement-dependent cytotoxicity" or "CDC" is another method of cell killing that can be directed by antibodies. IgM is the most effective isotype for complement activation. Both IgG1 and IgG3 are highly effective in inducing CDC via the classical complement activation pathway. Preferably, in this cascade, formation of an antigen-antibody complex results in the uncloaking of multiple adjacent C1q binding sites on the CH2 domain of the participating antibody molecule, such as an IgG molecule (C1q is one of the three subcomponents of complement C1). Preferably, these uncloaked C1q binding sites convert a previously low affinity C1q-IgG interaction into one of high avidity, which triggers a cascade of events involving a series of other complement proteins, resulting in the proteolytic release of the effector cell chemotactic / activating agents C3a and C5a. Preferably, the complement cascade ends with the formation of the membrane attack complex, which creates pores in the cell membrane that facilitate the free passage of water and solutes into and out of the cell.

[0170] The term "Toll-like receptors" or "TLRs" refers to a class of proteins that play an important role in the innate immune system. They are single transmembrane non-catalytic receptors that are typically expressed in sentinel cells, such as macrophages and dendritic cells, that recognize structurally conserved molecules derived from microorganisms. When these microorganisms breach physical barriers, such as the skin or intestinal mucosa, they are recognized by TLRs, which activate immune cell responses.

[0171] In one embodiment of the invention, a nucleic acid, such as an RNA, encoding an antigen is administered to a subject, where an antigen translation product of the nucleic acid can be formed in the subject's cells, and the product can be presented to the immune system for stimulation of an immune response.

[0172] Alternatively, the invention contemplates embodiments in which nucleic acids expressing the antigens described herein are introduced into cells, such as antigen-presenting cells, ex vivo, and the antigen-presenting cells, e.g., taken from a patient and, optionally, clonally expanded ex vivo, are transplanted back into the same patient. The transfected cells may be reintroduced into the patient using any means known in the art, preferably in sterile form by intravenous, intracavitary, intraperitoneal or intratumoral administration.

[0173] The term "nucleic acid" as used herein is intended to include deoxyribonucleic acid (DNA) or ribonucleic acid (RNA), e.g., cDNA, mRNA, recombinantly produced and chemically synthesized molecules. Nucleic acids may be single-stranded or double-stranded. According to the present invention, RNA includes in vitro transcribed RNA (IVT RNA) or synthetic RNA. According to the present invention, a nucleic acid is preferably an isolated nucleic acid. Furthermore, the nucleic acids described herein may be recombinant molecules.

[0174] The term "isolated nucleic acid" means, according to the present invention, that the nucleic acid has been (i) amplified in vitro, e.g. by polymerase chain reaction (PCR), (ii) recombinantly produced by cloning, (iii) purified, e.g. by cleavage and separation by gel electrophoresis, or (iv) synthesized, e.g. by chemical synthesis. The nucleic acid may be used for introduction into a cell, i.e. for transfection of a cell, e.g. in the form of RNA, which may be prepared by in vitro transcription from a DNA template. Furthermore, the RNA may be modified before application, e.g. by stabilizing sequences, capping and polyadenylation.

[0175] In the context of the present invention, the term "DNA" refers to a molecule that comprises, and preferably is composed entirely or substantially of, deoxyribonucleotide residues. "Deoxyribonucleotide" refers to a nucleotide that lacks a hydroxyl group at the 2' position of the β-D-ribofuranosyl group. The term "DNA" includes isolated DNA, such as partially or completely purified DNA, essentially pure DNA, synthetic DNA, and recombinantly produced DNA, and includes modified DNA that differs from naturally occurring DNA by the addition, deletion, substitution, and / or alteration of one or more nucleotides. Such modifications may include the addition of non-nucleotide material, for example to the end of the DNA, or internally, for example, at one or more nucleotides of the DNA. Nucleotides in a DNA molecule may also include non-standard nucleotides, such as non-naturally occurring nucleotides or chemically synthesized nucleotides. These modified DNAs may be referred to as analogs or analogs of naturally occurring DNA.

[0176] In the context of the present invention, the term "RNA" refers to a molecule that comprises ribonucleotide residues, preferably composed entirely or substantially of ribonucleotide residues. "Ribonucleotide" refers to a nucleotide that has a hydroxyl group at the 2' position of a β-D-ribofuranosyl group. The term includes double-stranded RNA, single-stranded RNA, isolated RNA, e.g. partially or completely purified RNA, essentially pure RNA, synthetic RNA, recombinantly produced RNA, and modified RNA that differs from naturally occurring RNA by the addition, deletion, substitution and / or alteration of one or more nucleotides. Such modifications may include the addition of non-nucleotide material, e.g. to the end of the RNA, or internally, e.g. in one or more nucleotides of the RNA. Nucleotides in an RNA molecule may also include non-standard nucleotides, such as non-naturally occurring nucleotides or chemically synthesized nucleotides or deoxynucleotides. These modified RNAs may be referred to as analogs or analogs of naturally occurring RNA. According to the present invention, the term "RNA" includes and preferably relates to "mRNA", which means "messenger RNA", and relates to transcripts that may be generated using DNA as a template and that code for peptides or proteins. An mRNA typically comprises a 5' untranslated region (5'-UTR), a protein or peptide coding region, and a 3' untranslated region (3'-UTR). An mRNA has a limited half-life in cells and in vivo. Preferably, the mRNA is produced by in vitro transcription using a DNA template. In one embodiment of the invention, the RNA is obtained by in vitro transcription or chemical synthesis. In vitro transcription methods are known to those skilled in the art. For example, there are various in vitro transcription kits available commercially.

[0177] According to the present invention, the stability and translation efficiency of the RNA can be modified as necessary. For example, the RNA can be stabilized and its translation increased by one or more modifications that have a stabilizing effect and / or increase the translation efficiency of the RNA. To increase the expression of the RNA used according to the present invention, it can be modified in the coding region, i.e. in the sequence that codes for the expressed peptide or protein, preferably without changing the sequence of the expressed peptide or protein, to increase the GC content to increase mRNA stability and to perform codon optimization, thus enhancing translation in the cell.

[0178] The term "modification" in the context of RNA as used in the present invention includes any modification of RNA that is not naturally occurring in said RNA.

[0179] In one embodiment of the invention, the RNA used according to the invention is free of uncapped 5'-triphosphates. Removal of such uncapped 5'-triphosphates can be achieved by treating the RNA with a phosphatase.

[0180] The RNA of the present invention may have modified ribonucleotides to increase its stability and / or reduce its cytotoxicity.For example, in one embodiment, 5-methylcytidine is partially or completely, preferably completely, replaced by cytidine in the RNA used by the present invention.Alternatively or additionally, in one embodiment, pseudouridine is partially or completely, preferably completely, replaced by uridine in the RNA used by the present invention.

[0181] In one embodiment, the term "modification" relates to providing an RNA with a 5' cap or a 5' cap analog. The term "5' cap" refers to the cap structure found at the 5' end of an mRNA molecule, and generally consists of a guanosine nucleotide linked to the mRNA via an unusual 5' to 5' triphosphate bond. In one embodiment, the guanosine is methylated at position 7. The term "conventional 5' cap" refers to a naturally occurring RNA 5' cap, preferably a 7-methylguanosine cap (m 7 In the context of the present invention, the term "5' cap" includes 5' cap analogs that resemble an RNA cap structure and have been modified to have the ability to stabilize an RNA and / or that, when attached thereto, preferably enhance translation of the RNA in vivo and / or in a cell.

[0182] The RNA may comprise further modifications. For example, further modifications of the RNA used in the present invention may be the extension or truncation of the naturally occurring poly(A) tail, or the modification of the 5'- or 3'-untranslated region (UTR), such as the introduction of a UTR that is not related to the coding region of said RNA, for example the insertion of or replacement of an existing 3'-UTR with one or more, preferably two copies of a 3'-UTR from a globin gene, such as alpha2-globin, alpha1-globin, beta-globin, preferably beta-globin, more preferably human beta-globin.

[0183] RNA with an unmasked polyA sequence is translated more efficiently than RNA with a masked polyA sequence. The term "poly(A) tail" or "polyA sequence" refers to a sequence of adenyl (A) residues typically located at the 3' end of an RNA molecule, and "unmasked polyA sequence" means that the polyA sequence at the 3' end of the RNA molecule ends with the A of the polyA sequence and is not followed by any nucleotides other than A located at the 3' end, i.e. downstream, of the polyA sequence. Furthermore, a long polyA sequence of about 120 base pairs provides optimal RNA transcript stability and translation efficiency.

[0184] Thus, in order to increase the stability and / or expression of the RNA used according to the invention, it may be modified to be present with a polyA sequence, preferably having a length of 10 to 500, more preferably 30 to 300, even more preferably 65 to 200, in particular 100 to 150 adenosine residues. In a particularly preferred embodiment, the polyA sequence has a length of about 120 adenosine residues. In order to further increase the stability and / or expression of the RNA used according to the invention, the polyA sequence may be unmasked.

[0185] The term "stability" of an RNA relates to the "half-life" of the RNA. "Half-life" relates to the period required to eliminate half of the activity, amount, or number of a molecule. In the context of the present invention, the half-life of an RNA is an indication of the stability of said RNA. The half-life of an RNA may affect the "duration of expression" of the RNA. An RNA with a long half-life may be expected to be expressed for a long period of time.

[0186] Of course, if it is desired to reduce the stability and / or translation efficiency of an RNA according to the present invention, the RNA can be modified to interfere with the function of the above-mentioned elements that increase RNA stability and / or translation efficiency.

[0187] The nucleic acids described herein may be included in a vector that can be used to deliver the nucleic acid inside a cell. As used herein, the term "vector" includes any vector known to those skilled in the art, including plasmid vectors, cosmid vectors, phage vectors, such as lambda phage, viral vectors, such as adenovirus or baculovirus vectors, retro- or lentivirus vectors, transposon or artificial chromosome vectors, such as bacterial artificial chromosomes (BAC), yeast artificial chromosomes (YAC), or P1 artificial chromosomes (PAC). Such vectors include expression and cloning vectors. Expression vectors include plasmids and viral vectors, and generally contain the desired coding sequence and appropriate DNA sequences required for the expression of the operably linked coding sequence in a particular host organism (e.g., bacteria, yeast, plants, insects, or mammals) or in an in vitro expression system. Cloning vectors are generally used to manipulate and amplify a given desired DNA fragment, and may lack functional sequences required for the expression of the desired DNA fragment.

[0188] Those skilled in the art of molecular biology generally know how to use promoter, enhancer, and cell type combinations for protein expression. The promoters used can be constitutive, tissue-specific, inducible, and / or useful under appropriate conditions to direct high levels of expression of the introduced nucleic acid segment. The promoters can be heterologous or endogenous. Constitutive promoter sequences that can be used by the present invention include, but are not limited to, immediate early cytomegalovirus (CMV) promoter sequences, simian virus 40 (SV40) early promoter, mouse mammary tumor virus (MMTV), human immunodeficiency virus (HIV) long terminal repeat (LTR) promoter, Moloney virus promoter, avian leukosis virus promoter, Epstein-Barr virus immediate early promoter, Rous sarcoma virus promoter, and human gene promoters, such as, but not limited to, actin promoter, myosin promoter, hemoglobin promoter, and muscle creatine promoter. Furthermore, the present invention should not be limited to the use of constitutive promoters. Inducible promoters are also considered as part of the present invention. The use of an inducible promoter in the present invention provides a molecular switch that can turn on the expression of an operably linked polynucleotide sequence when such expression is desired, or turn off the expression when expression is not desired. Examples of inducible promoters include, but are not limited to, metallothionine promoters, glucocorticoid promoters, progesterone promoters, and tetracycline promoters. Furthermore, the present invention includes the use of tissue-specific promoters, which are active only in the desired tissue. Tissue-specific promoters are well known in the art and include, but are not limited to, HER-2 promoters and PSA-related promoter sequences.

[0189] The nucleic acid can be transferred into a host cell by physical, chemical, or biological means.

[0190] Physical methods for introducing nucleic acid into host cells include calcium phosphate precipitation, lipofection, particle bombardment, microinjection, electroporation, and the like.

[0191] Biological methods for introducing nucleic acid of interest into host cells include the use of DNA and RNA vectors.Viral vectors, especially retroviral vectors, have become the most widely used method for inserting genes into mammalian, e.g., human cells.Other viral vectors can be derived from lentiviruses, poxviruses, herpes simplex virus I, adenoviruses and adeno-associated viruses, etc.

[0192] Chemical means for introducing nucleic acid into host cells include colloidal dispersion systems, such as macromolecular complexes, nanocapsules, microspheres, beads, and lipid-based systems, including oil-in-water emulsions, micelles, mixed micelles, and liposomes. A preferred colloidal system for use as a delivery vehicle in vitro and in vivo is a liposome (i.e., an artificial membrane vesicle). The preparation and use of such systems are well known in the art.

[0193] "Encoding" refers to the inherent property of a particular sequence of nucleotides in a nucleic acid to serve as a template for the synthesis of other polymers and macromolecules in biological processes having a defined nucleotide sequence or a defined amino acid sequence. Thus, a nucleic acid encodes a protein when expression (translation and optionally transcription) of the nucleic acid produces a protein in a cell or other biological system.

[0194] The term "expression" is used according to the present invention in its most general sense and includes the production of RNA and / or peptides or polypeptides, for example by transcription and / or translation. With respect to RNA, the terms "expression" or "translation" relate in particular to the production of peptides or polypeptides. It also includes partial expression of a nucleic acid. Furthermore, expression can be transient or stable.

[0195] In the context of the present invention, the term "transcription" refers to the process in which the genetic code in a DNA sequence is transcribed into RNA. The RNA can then be translated into a protein. According to the present invention, the term "transcription" includes "in vitro transcription", where the term "in vitro transcription" refers to a process in which RNA, in particular mRNA, is synthesized in vitro in a cell-free system, preferably using a suitable cell extract. Preferably, a cloning vector is applied for the generation of the transcript. These cloning vectors are generally called transcription vectors and according to the present invention are included in the term "vector". According to the present invention, the RNA used in the present invention is preferably in vitro transcribed RNA (IVT-RNA) and can be obtained by in vitro transcription of a suitable DNA template. The promoter for controlling the transcription can be any promoter for any RNA polymerase. Particular examples of RNA polymerases are T7, T3 and SP6 RNA polymerases. Preferably, the in vitro transcription according to the present invention is controlled by a T7 or SP6 promoter. The DNA template for in vitro transcription can be obtained by cloning a nucleic acid, in particular a cDNA, and introducing it into a suitable vector for in vitro transcription. cDNA can be obtained by reverse transcription of RNA.

[0196] The term "translation" according to the present invention relates to the process in a cell's ribosomes whereby strands of messenger RNA direct the assembly of amino acid sequences to make peptides or polypeptides.

[0197] Expression control sequences or regulatory sequences that may be operably linked to a nucleic acid according to the present invention may be homologous or heterologous with respect to the nucleic acid. A coding sequence and a regulatory sequence are "operably" linked if they are covalently linked, and the transcription or translation of the coding sequence is under the control or influence of the regulatory sequence. If a coding sequence is translated into a functional protein and the regulatory sequence is operably linked to the coding sequence, the introduction of the regulatory sequence directs the transcription of the coding sequence without causing a reading frame shift in the coding sequence or preventing the coding sequence from being translated into the desired protein or peptide.

[0198] The term "expression control sequence" or "regulatory sequence" according to the present invention includes promoters, ribosome binding sequences, and other regulatory elements that control the transcription of a nucleic acid or the translation of an induced RNA. In certain embodiments of the present invention, the regulatory sequence can be controlled. The exact structure of a regulatory sequence may vary depending on the species and cell type, but generally includes 5' non-transcribed sequences and 5' and 3' non-translated sequences involved in the initiation of transcription or translation, such as the TATA box, capping sequence, CAAT sequence, etc. In particular, the 5' non-transcribed regulatory sequence includes a promoter region that includes a promoter sequence for transcriptional control of an operably linked gene. Regulatory sequences can also include enhancer sequences or upstream activator sequences.

[0199] According to the invention, a nucleic acid, such as an RNA, encoding a peptide or protein that is taken up or introduced, i.e. transfected or transduced, into a cell, which may be present in vitro or in a subject, preferably results in expression of said peptide or protein. The cell may express the encoded peptide or protein intracellularly (e.g. in the cytoplasm and / or nucleus), may secrete the encoded peptide or protein, or may express it on its surface.

[0200] In accordance with the present invention, terms such as "expressing a nucleic acid" and "encoding a nucleic acid" or similar terms are used interchangeably herein and mean that, with respect to a particular peptide or polypeptide, a nucleic acid can be expressed to produce said peptide or polypeptide when present in the appropriate environment, preferably within a cell.

[0201] Terms such as "transfer", "introduce", "transfect" or "transduce" are used interchangeably herein and relate to the introduction of exogenous or heterologous nucleic acids, such as nucleic acids, in particular RNA, into cells. According to the invention, the cells may be in vitro or in vivo, for example the cells may form part of an organ, tissue and / or organism. According to the invention, transfection may be transient or stable. In some applications of transfection, it is sufficient that the transfected genetic material is only expressed transiently. Usually, the nucleic acid introduced in the transfection process is not integrated into the nuclear genome, so that the foreign nucleic acid is diluted or degraded by mitosis. Cell lines that allow episomal amplification of the nucleic acid greatly reduce the dilution rate. If it is desired that the transfected nucleic acid indeed remains in the genome of the cell and its daughter cells, stable transfection must be performed. RNA can be transfected into cells to transiently express its encoded protein.

[0202] A "fragment" in reference to a nucleic acid sequence relates to a sequence that represents a portion of the nucleic acid sequence, i.e. a nucleic acid sequence that is truncated at the 5' and / or 3' end. A fragment of a nucleic acid sequence preferably comprises at least 6, in particular at least 8, at least 12, at least 15, at least 20, at least 30, at least 50 or at least 100 consecutive nucleotides of the nucleic acid sequence.

[0203] The present invention also includes "variants" of the nucleic acids or nucleic acid sequences, such as the immunostimulatory RNA molecules described herein.

[0204] According to the present invention, nucleic acid variants include single or multiple nucleotide deletions, additions, mutations, and / or insertions compared to the reference nucleic acid. Deletions include removal of one or multiple nucleotides from the reference nucleic acid. Addition variants include 5' and / or 3' terminal fusions of one or multiple nucleotides, such as 1, 2, 3, 5, 10, 20, 30, 50, or more nucleotides. Mutations can include, but are not limited to, substitutions in which at least one nucleotide in a sequence is removed and another nucleotide is inserted in its place (such as transversions and transitions), abasic sites, crosslinked sites, and chemically altered or modified bases. Insertions include addition of at least one nucleotide to the reference nucleic acid.

[0205] Preferably, the degree of identity between a given nucleic acid sequence and a nucleic acid sequence that is a variant of the given nucleic acid sequence will be at least about 60%, 65%, 70%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99%. The degree of identity is preferably provided over a nucleic acid region that is at least about 10%, at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90% or about 100% of the entire length of the reference nucleic acid sequence. For example, if the reference nucleic acid sequence consists of 200 nucleotides, the degree of identity is preferably provided over at least about 20, at least about 40, at least about 60, at least about 80, at least about 100, at least about 120, at least about 140, at least about 160, at least about 180, or about 200 nucleotides, preferably consecutive nucleotides. The degree of identity is preferably provided over a segment of at least 80, at least 100, at least 120, at least 150, at least 180, at least 200, or at least 250 nucleotides. In preferred embodiments, the degree of identity is provided over the entire length of the reference nucleic acid sequence.

[0206] "Sequence identity" between two nucleic acid sequences indicates the percentage of nucleotides that are identical between the sequences.

[0207] The term "% identity" refers in particular to the percentage of nucleotides that are identical in optimal alignment between the two sequences being compared, said percentage being purely statistical, and the differences between the two sequences may be randomly distributed over the entire length of the sequences, and the compared sequence may contain additions or deletions compared to the reference sequence in order to obtain optimal alignment between the two sequences. Usually, the comparison of two sequences is carried out by comparing said sequences after optimal alignment over a segment or "comparison window" in order to identify local regions of corresponding sequences. Optimal alignment for comparison can be performed manually or using the local homology algorithm of Smith and Waterman, 1981, Ads App. Math. 2, 482, using the local homology algorithm of Neddleman and Wunsch, 1970, J. Mol. Biol. 48, 443, and using a similarity search algorithm according to Pearson and Lipman, 1988, Proc. Natl Acad. Sci. USA 85, 2444, or using computer programs which use said algorithms (GAP, BESTFIT, FASTA, BLAST P, BLAST N and TFASTA in Wisconsin Genetics Software Package, Genetics Computer Group, 575 Science Drive, Madison, Wis).

[0208] The percentage of identity is obtained by determining the number of identical positions where the compared sequences correspond, dividing this number by the number of positions compared and multiplying this result by 100.

[0209] A variant of a particular nucleic acid sequence or a nucleic acid sequence having a particular degree of identity to a particular nucleic acid sequence preferably retains at least one functional property of the particular sequence, and is preferably functionally equivalent to the particular sequence, e.g. the nucleic acid sequence exhibits properties identical or similar to those of the particular nucleic acid sequence.

[0210] One important property includes the ability to act as an adjuvant or immunostimulant, particularly when administered in combination with an antigen or a nucleic acid encoding an antigen.

[0211] According to the present invention, the term "peptide" refers to a substance which comprises two or more, preferably three or more, preferably four or more, preferably six or more, preferably eight or more, preferably ten or more, preferably thirteen or more, preferably sixteen or more, preferably twenty one or more, preferably up to 8, 10, 20, 30, 40 or 50, in particular up to 100 amino acids covalently linked by peptide bonds.

[0212] The term "protein" refers to large peptides, i.e., polypeptides, preferably peptides having more than 100 amino acid residues, although in general the terms "peptide," "polypeptide," and "protein" are synonymous and are used interchangeably herein.

[0213] The present invention also includes "variants" of the peptide, protein, or amino acid sequences described herein.

[0214] For the purposes of the present invention, "variants" of an amino acid sequence include amino acid insertion variants, amino acid addition variants, amino acid deletion variants and / or amino acid substitution variants.

[0215] Amino acid insertion variants include the insertion of a single or two or more amino acids in a particular amino acid sequence. In the case of amino acid sequence variants with insertions, one or more amino acid residues are inserted at specific sites in the amino acid sequence, although random insertion with appropriate screening of the resulting product is also possible.

[0216] Amino acid addition variants include amino and / or carboxy terminal fusions of one or more amino acids, such as 1, 2, 3, 5, 10, 20, 30, 50, or more amino acids.

[0217] Amino acid deletion variants are characterized by the removal of one or more amino acids from the sequence, such as the removal of 1, 2, 3, 5, 10, 20, 30, 50 or more amino acids. The deletion may be anywhere in the protein. Amino acid deletion variants that include deletions at the N-terminus and / or C-terminus of the protein are also referred to as N-terminal and / or C-terminal truncation variants.

[0218] Amino acid substitution variants are characterized in that at least one residue in the sequence has been removed and another has been inserted in its place. It is preferred that the modification is at a position in the amino acid sequence that is not conserved between homologous proteins or peptides and / or replaces an amino acid with another with similar properties. Preferably, the amino acid changes in the protein variants are conservative amino acid changes, i.e., substitutions of similarly charged or uncharged amino acids. Conservative amino acid changes include the substitution of one of a family of amino acids that are related in their side chains. Naturally occurring amino acids are generally classified into four families: acidic (aspartic acid, glutamic acid), basic (lysine, arginine, histidine), non-polar (alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan), and uncharged polar (glycine, asparagine, glutamine, cysteine, serine, threonine, tyrosine) amino acids. Phenylalanine, tryptophan, and tyrosine are sometimes collectively classified as aromatic amino acids.

[0219] Preferably, the degree of similarity, preferably identity, between a given amino acid sequence and an amino acid sequence that is a variant of the given amino acid sequence will be at least about 60%, 65%, 70%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99%. The degree of similarity or identity is preferably given over an amino acid region that is at least about 10%, at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90% or about 100% of the entire length of the reference amino acid sequence. For example, if the reference amino acid sequence consists of 200 amino acids, the degree of similarity or identity is given for at least about 20, at least about 40, at least about 60, at least about 80, at least about 100, at least about 120, at least about 140, at least about 160, at least about 180, or about 200 amino acids, preferably consecutive amino acids. The degree of similarity or identity is preferably given for a segment of at least 80, at least 100, at least 120, at least 150, at least 180, at least 200, or at least 250 amino acids. In a preferred embodiment, the degree of similarity or identity is given for the entire length of the reference amino acid sequence. Alignment to determine sequence similarity, preferably sequence identity, can be performed using tools known in the art, preferably using best sequence alignment, e.g., using Align, using standard settings, preferably EMBOSS::needle, Matrix:Blosum62, Gap Open 10.0, Gap Extend 0.5.

[0220] "Sequence similarity" indicates the percentage of amino acids that are identical or represent conservative amino acid substitutions. "Sequence identity" between two amino acid sequences indicates the percentage of amino acids that are identical between the sequences.

[0221] The term "% identity" is intended to refer in particular to the percentage of amino acid residues that are identical in optimal alignment between the two sequences being compared, said percentage being purely statistical, the differences between the two sequences may be randomly distributed over the entire length of the sequences, and the compared sequence may contain additions or deletions compared to the reference sequence in order to obtain optimal alignment between the two sequences. Usually, the comparison of two sequences is carried out by comparing said sequences after optimal alignment over a segment or "comparison window" in order to identify local regions of corresponding sequences. Optimal alignment for comparison can be performed manually or using the local homology algorithm of Smith and Waterman, 1981, Ads App. Math. 2, 482, using the local homology algorithm of Neddleman and Wunsch, 1970, J. Mol. Biol. 48, 443, and using a similarity search algorithm according to Pearson and Lipman, 1988, Proc. Natl Acad. Sci. USA 85, 2444, or using computer programs which use said algorithms (GAP, BESTFIT, FASTA, BLAST P, BLAST N and TFASTA in Wisconsin Genetics Software Package, Genetics Computer Group, 575 Science Drive, Madison, Wis).

[0222] The percent identity is obtained by determining the number of corresponding identical positions in the compared sequences, dividing this number by the number of positions compared and multiplying this result by 100.

[0223] According to the invention, homologous amino acid sequences exhibit an identity of at least 40%, in particular at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, preferably at least 95%, at least 98 or at least 99% of the amino acid residues.

[0224] According to the invention, variants, fragments, parts or portions of an amino acid sequence, peptide or protein preferably have a functional property of the amino acid sequence, peptide or protein, respectively, from which it is derived, i.e. are functionally equivalent. In one embodiment, variants, fragments, parts or portions of an amino acid sequence, peptide or protein, respectively, are immunologically equivalent to the amino acid sequence, peptide or protein from which it is derived. In one embodiment, the functional property is an immunological property.

[0225] The present invention includes derivatives of the peptides or proteins described herein within the terms "peptide" and "protein". According to the present invention, "derivatives" of proteins and peptides are modified forms of proteins and peptides. Such modifications include any chemical modification, including single or multiple substitutions, deletions and / or additions of any molecules associated with the protein or peptide, such as carbohydrates, lipids and / or proteins. In one embodiment, "derivatives" of proteins or peptides include modified analogs resulting from glycosylation, acetylation, phosphorylation, amidation, palmitoylation, myristoylation, isoprenylation, lipidation, alkylation, derivatization, introduction of protecting / blocking groups, proteolytic cleavage or binding to an antibody or another cellular ligand. The term "derivatives" also extends to all functional chemical equivalents of said proteins and peptides. Preferably, the modified peptides have increased stability and / or increased immunogenicity.

[0226] The term "derived from" means according to the invention that a particular entity, in particular a particular sequence, is present in the object, in particular an organism or molecule, from which it originates. In the case of amino acid or nucleic acid sequences, in particular a particular sequence region, "derived from" means in particular that the relevant amino acid or nucleic acid sequence is derived from the amino acid or nucleic acid sequence in which it is present.

[0227] The term "cell" or "host cell" preferably refers to an intact cell, i.e. a cell with an intact membrane that has not released its normal intracellular components such as enzymes, organelles, or genetic material. An intact cell is preferably a viable cell, i.e. a living cell capable of carrying out its normal metabolic functions. Preferably, said term relates according to the present invention to any cell that can be transformed or transfected with an exogenous nucleic acid. The term "cell" according to the present invention includes prokaryotic cells (e.g., E. coli) or eukaryotic cells (e.g., dendritic cells, B cells, CHO cells, COS cells, K562 cells, HEK293 cells, HELA cells, yeast cells, and insect cells). Exogenous nucleic acids can be found in cells (i) distributed freely as such, (ii) incorporated in a recombinant vector, or (iii) integrated into the host cell genome or mitochondrial DNA. Mammalian cells are particularly preferred, such as cells of human, mouse, hamster, pig, goat, and primate origin. The cells may be derived from multiple tissue types and include primary cells and cell lines.

[0228] Cells containing the nucleic acid, eg, cells transfected with the nucleic acid, preferably express the peptide or protein encoded by the nucleic acid.

[0229] The term "proliferation" refers to the process by which a specific entity is multiplied. In one embodiment of the invention, the term is used in the context of an immunological response in which lymphocytes are stimulated by an antigen, proliferate, and specific lymphocytes that recognize said antigen are expanded. Preferably, clonal expansion results in differentiation of lymphocytes.

[0230] As used herein, "isolated" is intended to refer to a molecule that is substantially free of other molecules, such as other cellular material.

[0231] The term "recombinant" in the context of the present invention means "made through genetic engineering." Preferably, a "recombinant," such as a recombinant cell or nucleic acid, in the context of the present invention is not naturally occurring.

[0232] The term "naturally occurring" as used herein refers to the fact that a substance can be found in nature. For example, a peptide or nucleic acid that is present in an organism (including viruses), can be isolated from a natural source, and has not been intentionally modified by man in the laboratory is naturally occurring.

[0233] Terms such as "reduce", "inhibit" or "decrease" refer to the ability to cause an overall decrease in levels, preferably by 5% or more, 10% or more, 20% or more, more preferably by 50% or more, and most preferably by 75% or more. These terms include complete or essentially complete inhibition, i.e., a reduction to zero or essentially zero.

[0234] Terms such as "increase," "enhance," "promote," or "stimulate" relate to the ability to cause an overall increase in levels, preferably by 5% or more, 10% or more, 20% or more, 50% or more, 75% or more, 100% or more, 200% or more, or 500% or more. These terms can relate to an increase, enhancement, promotion, or stimulation from zero or an unmeasurable or undetectable level to a level greater than zero or a measurable or detectable level. Alternatively, these terms can also mean that there is a given level prior to the increase, enhancement, promotion, or stimulation, and that the level is higher after the increase, enhancement, promotion, or stimulation.

[0235] The agents and compositions described herein can be used to treat a subject having a disease, e.g., a disease characterized by the presence of an antigen or diseased cells expressing the antigen. Particularly preferred diseases are cancer diseases. The agents and compositions described herein can also be used for immunization or vaccination to prevent the diseases described herein.

[0236] The term "disease" refers to an abnormal condition that affects an individual's body. Disease is often interpreted as a medical condition associated with certain symptoms or signs. Disease may be caused by factors resulting from external causes, such as infectious diseases, or may be caused by internal dysfunction, such as autoimmune diseases. In humans, "disease" is often used more broadly to refer to any condition that causes pain, impairment, distress, social problems, or death to the afflicted individual, or causes similar problems to those in contact with the individual. In this broad sense, disease often includes injuries, disabilities, disorders, syndromes, infections, single symptoms, deviant behavior, and atypical variations in structure and function, although in other contexts and for other purposes, these may be considered as distinct categories. Diseases usually affect individuals not only physically but also emotionally, as living with many diseases can change one's outlook on life and personality. According to the present invention, the term "disease" includes infectious diseases and cancer diseases. References herein to cancer or to specific forms of cancer also include its cancer metastases.

[0237] The diseases to be treated according to the present invention are preferably antigen-mediated or antigen-associated diseases.

[0238] "Antigen-associated disease" or "antigen-associated disease" refers to any disease related to an antigen, e.g., a disease characterized by the presence of the antigen or a cell expressing the antigen. The antigen-associated disease may be an infectious disease, or a cancer disease, or simply a cancer. As mentioned above, the antigen may be a disease-associated antigen, e.g., a tumor-associated antigen, a viral antigen, or a bacterial antigen.

[0239] The term "infectious disease" refers to any disease that can be transmitted from individual to individual or organism to organism and is caused by a microbial agent. Infectious diseases are known in the art and include, for example, viral diseases, bacterial diseases, or parasitic diseases, which are caused by viruses, bacteria, and parasites, respectively. In this regard, infectious diseases can be, for example, hepatitis, sexually transmitted diseases (e.g., chlamydia or gonorrhea), tuberculosis, HIV / Acquired Immune Deficiency Syndrome (AIDS), diphtheria, Hepatitis B, Hepatitis C, cholera, Severe Acute Respiratory Syndrome (SARS), avian influenza, and influenza.

[0240] The term "cancer disease" or "cancer" refers to or describes a physiological condition in an individual that is typically characterized by unregulated cell proliferation. Examples of cancer include, but are not limited to, carcinoma, lymphoma, blastoma, sarcoma, and leukemia. More specifically, examples of such cancer include bone cancer, blood cancer, lung cancer, liver cancer, pancreatic cancer, skin cancer, head and neck cancer, cutaneous or intraocular melanoma, uterine cancer, ovarian cancer, rectal cancer, anal cancer, stomach cancer, colon cancer, breast cancer, prostate cancer, uterine cancer, carcinoma of the genital and reproductive organs, Hodgkin's disease, esophageal cancer, small intestine cancer, endocrine system cancer, thyroid cancer, parathyroid cancer, adrenal cancer, soft tissue sarcoma, bladder cancer, kidney cancer, renal cell carcinoma, renal pelvis cancer, central nervous system (CNS) neoplasms, neuroectodermal cancer, spinal axis tumor, glioma, meningioma, and pituitary adenoma. The term "cancer" according to the present invention also includes cancer metastasis. Preferably, a "cancer disease" is characterised by cells which express a tumour antigen, and a cancer cell expresses the tumour antigen.

[0241] In one embodiment, the cancer disease is a malignant disease characterized by the properties of anaplasia, invasiveness, and metastasis. Malignant tumors contrast with non-cancerous benign tumors in that malignant tumors are not self-limited in their growth, but can invade adjacent tissues and can spread to distant tissues (metastasize), whereas benign tumors lack these properties.

[0242] According to the present invention, the term "tumor" or "tumor disease" refers to an abnormal proliferation of cells (called neoplastic, neoplastic or neoplastic cells) that preferably form a swelling or lesion. By "tumor cells" is meant abnormal cells that grow by rapid uncontrolled cell proliferation and continue to grow after the stimulus that initiated the new growth has ceased. Tumors show a partial or complete lack of structural and functional coordination with normal tissue and usually form a distinct mass of tissue that may be benign, premalignant or malignant.

[0243] "Metastasis" means the spread of cancer cells from their original site to another part of the body. The formation of metastasis is a very complex process, depending on the detachment of malignant cells from the primary tumor, invasion of the extracellular matrix, penetration of the endothelial basement membrane to enter the body cavities and blood vessels, and then invasion of the target organ after transport by blood. Finally, the growth of new tumors, i.e. secondary or metastatic tumors, at the target site depends on angiogenesis. Tumor metastasis often occurs even after removal of the primary tumor, since tumor cells or components may remain and develop metastatic potential. In one embodiment, the term "metastasis" according to the present invention relates to "distant metastasis", which refers to metastasis away from the primary tumor and the regional lymph node system. In one embodiment, the term "metastasis" according to the present invention relates to lymph node metastasis.

[0244] Relapse or recurrence occurs when a person is once again affected by a condition that he or she previously suffered from. For example, if a patient suffers from a tumor disease, is successfully treated for said disease, and develops said disease again, the newly developed disease may be considered as a relapse or recurrence. However, according to the present invention, relapse or recurrence of a tumor disease does not necessarily occur at the site of the original tumor disease. Thus, for example, if a patient suffers from an ovarian tumor and is successfully treated, relapse or recurrence may be the development of an ovarian tumor or the development of a tumor at a site other than the ovary. Relapse or recurrence of a tumor also includes the situation where a tumor develops at a site other than the site of the original tumor, as well as at the site of the original tumor. Preferably, the original tumor that the patient was treated for is a primary tumor, and a tumor at a site other than the site of the original tumor is a secondary or metastatic tumor.

[0245] The term "treatment" or "therapeutic treatment" refers to any treatment that improves the health and / or extends (increases) the lifespan of an individual. The treatment may eliminate a disease in an individual, halt or delay the onset of a disease in an individual, inhibit or delay the onset of a disease in an individual, reduce the frequency or severity of symptoms in an individual, and / or reduce recurrence in an individual currently or previously affected by a disease.

[0246] The term "prophylactic treatment" or "preventive treatment" relates to any treatment intended to prevent the occurrence of a disease in an individual, particularly an individual at risk of the disease. The terms "prophylactic treatment" and "preventive treatment" are used interchangeably herein.

[0247] "At risk" refers to a subject, i.e., a patient, who is identified as having a higher than normal chance of developing a disease, particularly cancer, compared to the general population.In addition, a subject who has had or is currently suffering from a disease, particularly cancer, is at high risk of developing the disease, as they may continue to develop the disease.Subjects who currently have or have had cancer are also at high risk of cancer metastasis.

[0248] The term "immunotherapy" relates to the treatment of a disease or condition by inducing or enhancing an immune response. The term "immunotherapy" includes antigen immunization or vaccination, or tumor immunization or vaccination.

[0249] The terms "immunization" or "vaccination" describe the process of providing an individual with an antigen for the purpose of inducing an immune response, for example for therapeutic or prophylactic reasons.

[0250] The term "in vivo" refers to the situation in a subject.

[0251] The term "individual" or "subject" refers to vertebrates, particularly mammals. For example, mammals in the context of the present invention are humans, non-human primates, domesticated mammals, such as dogs, cats, sheep, cows, goats, pigs, horses, etc., laboratory animals, such as mice, rats, rabbits, guinea pigs, etc., and captive animals, such as zoo animals. The term "subject" also relates to non-mammalian vertebrates, such as birds, particularly domesticated birds, such as chickens, ducks, geese, turkeys, and fish, particularly farmed fish, such as salmon or catfish. The term "animal" as used herein also includes humans. Preferably, the term "patient" refers to an affected individual.

[0252] The term "autologous" is used to describe something that is derived from the same subject. For example, an "autologous graft" refers to a tissue or organ transplant that is derived from the same subject. Such procedures are advantageous because they overcome immunological barriers that would otherwise result in rejection.

[0253] The term "allogeneic" is used to describe something that is derived from different individuals of the same species. Two or more individuals are said to be allogeneic to one another if their genes at one or more loci are not identical.

[0254] The term "syngeneic" is used to describe individuals or tissues that have the same genotype, i.e., derived from identical twins or animals, or tissues thereof, of the same inbred line.

[0255] The term "xenogeneic" is used to describe something that is made up of multiple different elements. As an example, transplanting bone marrow from one individual into another individual constitutes a xenogeneic transplant. A xenogeneic gene is a gene that is derived from a source other than the subject.

[0256] The agents described herein may be administered in the form of any suitable pharmaceutical composition. The term "pharmaceutical composition" refers to a formulation containing a therapeutically effective agent or its salt, preferably together with pharmaceutical excipients such as buffers, preservatives and osmolality adjusting agents. The pharmaceutical composition is useful for treating or preventing a disease or disorder by administering the pharmaceutical composition to an individual. Pharmaceutical compositions are also known in the art as pharmaceutical formulations. Pharmaceutical compositions may be administered locally or systemically.

[0257] The term "systemic administration" refers to the administration of a therapeutically effective agent such that the agent becomes widely distributed in significant amounts within the body of an individual and exerts its biological effect. According to the present invention, administration is preferably by parenteral administration.

[0258] The term "parenteral administration" refers to administration of a therapeutically effective agent such that the agent does not pass through the intestine. The term "parenteral administration" includes, but is not limited to, intravenous, subcutaneous, intradermal, or intraarterial administration.

[0259] In certain embodiments, the antigen or nucleic acid encoding the antigen is administered before, simultaneously with, and / or after administration of the immunostimulatory RNA molecule described herein. The antigen or nucleic acid encoding the antigen and the immunostimulatory RNA molecule may be present in a common composition, i.e., mixed together. Furthermore, embodiments in which the antigen or nucleic acid encoding the antigen and the immunostimulatory RNA molecule are present together, but not in the same composition, are also envisaged by the present invention. Said embodiment particularly relates to a kit comprising at least two containers, one container containing a composition comprising the antigen or nucleic acid encoding the antigen and another container containing a composition comprising the immunostimulatory RNA molecule.

[0260] The pharmaceutical compositions of the invention may contain adjuvants other than the immunostimulatory RNA molecules described herein. Such adjuvants include oil emulsions (such as Freund's adjuvant), inorganic compounds (such as alum), bacterial products (such as Bordetella pertussis toxin), or heterologous compounds such as immunostimulatory complexes. Examples of adjuvants include, but are not limited to, saponin, incomplete Freund's adjuvant, complete Freund's adjuvant, tocopherol, or alum.

[0261] The pharmaceutical compositions of the present invention are generally applied in a "pharmaceutical effective amount" and in a "pharmaceutical acceptable formulation."

[0262] The term "pharmacologically effective amount" refers to an amount that alone or together with further doses achieves the desired response or the desired effect. In the case of the treatment of a particular disease, the desired response preferably relates to the inhibition of the course of the disease. This includes slowing down the progression of the disease, in particular halting or reversing the progression of the disease. The desired response in the treatment of a disease can also be the delay of the onset or prevention of the onset of said disease or condition. The effective amount of the compositions described herein will depend on the condition being treated, the severity of the disease, the individual parameters of the patient, including age, physiological state, size and weight, the duration of the treatment, the type of concomitant treatment (if any), the specific route of administration and similar factors. Thus, the dose administered of the compositions described herein can depend on various such parameters. If the patient's response is inadequate with the initial dose, a higher dose (or an effectively higher dose achieved by a different, more localized route of administration) can be used.

[0263] The term "pharmaceutically acceptable" refers to the non-toxicity of a material that does not interact with the action of the active ingredients of the pharmaceutical composition.

[0264] Pharmaceutical compositions of the invention may include salts, buffers, preservatives, carriers and optionally other therapeutic agents. Preferably, pharmaceutical compositions of the invention include one or more pharma- ceutically acceptable carriers, diluents and / or excipients.

[0265] The term "excipient" is intended to indicate any substance in a pharmaceutical composition that is not an active ingredient, such as a binder, lubricant, thickener, surfactant, preservative, emulsifier, buffer, flavoring agent, or coloring agent.

[0266] The term "diluent" refers to a diluting and / or dilutable agent. Further, the term "diluent" includes any one or more of a fluid, liquid or solid suspension and / or mixing medium.

[0267] The term "carrier" refers to one or more compatible solid or liquid fillers or diluents suitable for administration to humans. The term "carrier" refers to a natural or synthetic organic or inorganic component with which the active ingredient is combined to facilitate the application of the active ingredient. Preferably, the carrier component is a sterile liquid such as water or oil, including those derived from mineral oil, animal, or plant oils such as peanut oil, soybean oil, sesame oil, sunflower oil, etc. Salt solutions and aqueous dextrose and glycerin solutions can also be used as aqueous carrier compounds.

[0268] Pharmaceutically acceptable carriers or diluents for therapeutic use are well known in the pharmaceutical art and are described, for example, in Remington's Pharmaceutical Sciences, Mack Publishing Co. (A. R Gennaro, ed. 1985). Examples of suitable carriers include, for example, magnesium carbonate, magnesium stearate, talc, sugar, lactose, pectin, dextrin, starch, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose, low melting waxes, cocoa butter, etc. Examples of suitable diluents include ethanol, glycerol, and water.

[0269] The pharmaceutical carrier, excipient or diluent can be selected with regard to the intended route of administration and standard pharmaceutical practice. The pharmaceutical composition of the present invention may comprise any suitable binder, lubricant, suspending agent, coating agent, and / or solubilizing agent as, or in addition to, the carrier, excipient or diluent. Examples of suitable binders include starch, gelatin, natural sugars such as glucose, anhydrous lactose, free flowing lactose, beta-lactose, corn sweeteners, natural and synthetic gums such as acacia, tragacanth or sodium alginate, carboxymethylcellulose and polyethylene glycol. Examples of suitable lubricants include sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride, and the like. Preservatives, stabilizers, dyes, and even flavoring agents may be provided in the pharmaceutical composition. Examples of preservatives include sodium benzoate, sorbic acid, and esters of p-hydroxybenzoic acid. Antioxidants and suspending agents may also be used.

[0270] In one embodiment, the composition is an aqueous composition. The aqueous composition may optionally contain solutes, such as salts. In one embodiment, the composition is in the form of a lyophilized composition. The lyophilized composition can be obtained by lyophilizing each aqueous composition.

[0271] The agents and compositions provided herein may be used alone or in combination with other therapeutic regimens, such as surgery, radiation, chemotherapy and / or bone marrow transplantation (autologous, syngeneic, allogeneic or unrelated).

[0272] This invention is further illustrated by the following examples which should not be construed as limiting the scope of the invention. EXAMPLES

[0273] The techniques and methods used herein are either described herein or carried out in a manner known per se and as described, for example, in Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd ed. (1989) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. All methods, including the use of kits and reagents, are carried out according to manufacturer's information, unless otherwise indicated.

[0274] Materials and Methods [Control adjuvant and synthetic RNA oligonucleotide] TLR3 agonist poly(I:C), TLR7 / 8 ligand CL097, imidazoquinoline compound derivative R848, human TLR8 agonist ssRNA40 complexed with cationic lipid (LyoVec), human / mouse TLR9 agonist C-type CpG ODN2395 and human TLR9 agonist D-type CpG ODN2216 were purchased from Invivogen and used as control adjuvants in various experiments. Bafilomycin A1 (Invivogen) was used as an endosomal acidification inhibitor to block TLR activation. Complete and incomplete Freund's adjuvant (CFA / IFA) were used for subcutaneous control immunization according to the manufacturer's instructions (Sigma-Aldrich). Chemically synthesized isRNA NP71-Seq4 (Chem.Synth.) and poly(A) RNA as a control were purchased from Biomers.

[0275] [Virus-like particles (VLPs) derived from chimeric hepatitis B virus core antigen (HBcAg)] HBcAg-VLPs displaying a selected epitope (#A79) of the tumor-associated antigen (TAA) claudin-6 (CLDN6) on their surface were produced as described in Klamp, T. et al; Cancer Res 71(2), 1-12 (2011).

[0276] [In vitro transcription and purification of isRNA] The plasmid template for in vitro transcription (IVT) of isRNA was based on the pST1-A120 vector described in Kuhn, A. et al.; Gene Ther 17(8), 961-971 (2010). Selected influenza NP fragments were cloned between the SpeI and XhoI restriction sites using standard molecular biology methods. The resulting plasmids were linearized with XhoI (Fermentas, ThermoFisher) and purified by magnetic separation using Dynabeads MyOne Carboxylic Acid (Invitrogen). The subsequent IVT reaction was carried out according to the protocol previously described in Kreiter, S. et al.; Cancer Immunol Immunother 56, 1577-1587 (2007). Small-scale purification of IVT RNA was carried out on a silica-based membrane using the RNeasy Mini kit (QIAGEN) according to the manufacturer's instructions. For large-scale purification of IVT RNA, an FPLC-based method using a weak anion exchange column (HiTrap DEAE Sepharose FF, GE Healthcare) was applied (Easton, LE. et al., RNA 16(3), 647-653 (2010)).

[0277] [isRNA formulation] Depending on the use in in vitro or in vivo experiments, purified influenza NP-derived isRNA was formulated with cationic lipids that differ in their lipid / helper-lipid composition and surface charge (Kranz, LM. et al.; Nature 534, 396-401 (2016)). To ensure optimal cellular uptake in in vitro experiments, isRNA was formulated with liposomal composition F5, while composition F12 was used for in vivo experiments. The F12 formulation allowed high serum stability of isRNA after intravenous administration and splenic targeting of isRNA lipoplexes.

[0278] [mouse] Female Balb / cJRj and C57BL / 6 mice were obtained from Janvier Laboratories (France). BDCA2-DTR transgenic mice bred on a C57BL / 6 background, which express the diphtheria toxin receptor (DTR) specifically in plasmacytoid dendritic cells (pDCs), were purchased from Jackson Laboratory (USA). Mice were 6-10 weeks old at the start of the experiment. All animals were maintained under pathogen-free conditions.

[0279] [Isolation of human PBMCs and pDCs] Human PBMCs were freshly isolated from the blood of healthy male or female donors by Ficoll density gradient centrifugation as described in Lin, Z. et al.; Nature protocols 9, 1563-1577 (2014). Plasmacytoid dendritic cells (pDCs) were isolated from human PBMCs using MACS separation technology and the CD304 MicroBead Kit (Miltenyi Biotec).

[0280] [Isolation of mouse bone marrow cells, splenocytes, pDCs and cDCs] Mouse bone marrow cells (BMCs) were harvested from mouse femurs and tibias using standard protocols. Red blood cells were lysed by incubation with 5 ml of cold red blood cell (RBC) lysis buffer (Sigma-Aldrich) for 5 min, followed by the addition of 20 ml of 1× PBS (Gibco) to stop the lysis reaction. BMCs were pelleted by centrifugation and resuspended in cell culture medium. Mouse splenocytes were prepared as described in Kreiter, S. et al; Cancer Immunol Immunother 56, 1577-1587 (2007). pDCs were isolated from splenocytes by MACS separation using a Mouse pDC Isolation Kit II (Miltenyi Biotec). Common DCs (cDCs; CD11c high, B220 low) were generated from mouse BMCs by culturing in the presence of 20 ng / ml GM-CSF and 20 ng / ml IL4 (both from Peprotech) for 6 days.

[0281] [Cell culture] Primary human and mouse cells were cultured in RPMI 1640 supplemented with 10% (v / v) heat-inactivated FBS, 1% (v / v) non-essential amino acids (NEAA), 1% (v / v) sodium pyruvate, and 0.5% (v / v) penicillin / streptomycin solution. Chinese hamster ovary (CHO) K1 cells stably transfected with human CLDN6 or human CLDN9 were obtained from TRON gGmbH (Mainz, Germany) and cultured in DMEM supplemented with 10% (v / v) heat-inactivated FBS, 1 mg / ml G418, and 0.5% (v / v) penicillin / streptomycin solution. Wild-type human embryonic kidney (HEK) 293 cells or HEK293 cells stably co-expressing an NF-κB-inducible luciferase reporter gene and human TLR3, TLR7 or TLR8 were obtained from Invivogen and cultured in DMEM supplemented with 10% (v / v) heat-inactivated FBS, 1% (v / v) NEAA, 1% (v / v) sodium pyruvate and 0.5% (v / v) penicillin / streptomycin solution. Depending on the HEK293 transfectant used, the medium was additionally supplemented with Blasticidin (10 μg / ml), Zeocin (100 μg / ml) or Geneticin (250 μg / ml), all from Invivogen. FBS was purchased from Biochrom and all other cell culture reagents from Gibco.

[0282] [In vitro stimulation of cells with isRNA] Cell stimulation with isRNA or controls was performed in triplicate in 96-well plates (Corning) in a total volume of 200 μl. Unless otherwise stated, 5 × 10 cells were cultured per well. 5 of cells were used and stimulated with F5 formulated isRNA at a concentration of 0.167 μg / well or control reagent using the concentrations indicated in the examples for 12-16 hours at 37° C., 5% CO 2 .

[0283] [Cytokine detection] IFN-α and other selected cytokines were detected in human or mouse cell supernatants or mouse serum using commercially available ELISA kits (PBL Assay Science) or using Bio-Plex Pro kit III and cytokine-specific Bio-Plex coupled magnetic beads (BioRad) according to the manufacturer's instructions.

[0284] [In vivo stimulation and immune experiments] In the in vivo stimulation experiments, different amounts of F12-formulated isRNA were intravenously injected into the retrobulbar venous plexus of Balb / c mice. Depending on the experimental setup, blood samples for serum preparation were collected at various time points after injection, as shown in the examples. After clotting by centrifugation using standard protocols, serum was prepared and stored at -20°C until further use. Immunization experiments were performed by intravenous injection into the retrobulbar venous plexus of 20 μg of F12-formulated isRNA (total volume 100 μl) mixed with 50 μg of purified HBcAg-#A79VLP. Unless otherwise indicated, three injections were performed at 2-week intervals, and the final blood sample was taken 10 days after the last immunization. Serum preparation was performed as described above.

[0285] [Flow cytometry] The induction of specific antibodies against the native CLDN6 protein after immunization with adjuvanted or non-adjuvanted HBcAg-#A79 VLP was analyzed by flow cytometry. 5 CHO-K1 CLDN6 or CLDN9 cells were incubated for 1 h at 4° C. with polyclonal mouse antisera diluted 1:100 in FACS buffer (1× PBS, 5% (v / v) FBS, 5 mM EDTA). After three washing steps, cells were stained for 30 min at 4° C. with AlexaFluor647-conjugated goat anti-mouse IgG (H+L) secondary antibody (ThermoFisher) diluted 1:600 ​​in FACS buffer. Unbound antibody was removed by additional washing steps and viability was quantified using 7-AAD (Sigma). Fluorescent signals of live cells were detected by a FACS CantoII system (BD Biosciences).

[0286] [IFN-γELISpot analysis] 5×10 5 Freshly isolated mouse splenocytes were incubated in 96-well plates (Merck Millipore) coated with anti-IFN-γ monoclonal antibody (AN18 at 10 μg / ml, Mabtech) in the presence of 5 μg / ml #A79 or irrelevant peptide (JPT Peptide Technologies) for 18 h at 37°C. Plates were sequentially incubated with biotin-conjugated secondary anti-IFN-γ monoclonal antibody (R4-6A2, Mabtech) and ExtrAvidin-alkaline phosphatase (Sigma-Aldrich) before cytokine secretion was detected by addition of BCIP / NBT substrate (Sigma-Aldrich). Technical triplicates were performed for each group. Plates were scanned and analyzed using an ImmunoSpot S5 Versa ELISpot analyzer, ImmunoCapture software 6.3 and ImmunoSpot software 5.0.3 (all from Cellular Technology).

[0287] [Complement-dependent cytotoxicity (CDC) assay] To analyze antibody-mediated cytocidal effector functions, a complement-dependent cytotoxicity (CDC) assay was performed. CHO-K1 cells stably expressing human CLDN6 were cultured at 1 × 10 in 96-well plates (Corning Costar, Sigma-Aldrich). 4The cells were incubated at a concentration of 1000 x 1000 cells / well for 17.5 h at 37°C and 7.5% CO2. Subsequently, the cells were incubated with 5 μl of polyclonal mouse antiserum diluted in 32 μl of culture medium and 13 μl of human serum complement (Quidel Corporation) as a complement source in a total volume of 50 μl / well for 80 min. Mouse serum was obtained from an immunization experiment with differently adjuvanted HBcAg-#A79VLPs. A CLDN6-specific monoclonal antibody (Ganymed Pharmaceuticals AG, Mainz) at a concentration of 600 or 150 ng / ml was used as a positive control. Heat-inactivated human serum complement served as a negative control for confirmation of the complement-dependent antibody-mediated cell lysis effect. Untreated cells and cells lysed with Triton X-100 (Applichem) were used as benchmarks for 0% and 100% cell lysis, respectively. Cell viability was analyzed with the XTT assay-based cell proliferation kit II (Roche Diagnostics) according to the manufacturer's instructions. Absorbance at 480 nm was measured with an Infinite M200 Pro reader (Tecan). Antibody-mediated cytolytic activity was calculated by the following formula: % Cell Lysis = 100% - ((Signal Antiserum-Signal 100% Lysis / Signal Untreated Cells) x 100)

[0288] [Mouse immunoglobulin isotyping ELISA] Streptavidin-conjugated 96-well plates (Nunc, ThermoFisher) were coated with 100 ng / well of biotinylated #A79-peptide (JPT Peptide Technologies) for 1 h, after which uncoated surfaces were blocked with 300 μl / well of 1× PBS, 2% (v / v) FBS for 1 h and washed with 1× PBS, 0.05% Tween 20 (Sigma-Aldrich) using a HydroSpeed ​​plate washer (Tecan). Polyclonal mouse antisera were serially diluted 10-fold in 1× casein blocking buffer (Sigma-Aldrich), and 100 μl of diluted serum was added per well and incubated for 1 h with slight agitation on a shaking platform (Infors). After an additional washing step, bound antibodies were detected by incubation with a 1:5000 dilution of HRP-conjugated, goat anti-mouse IgG isotype-specific secondary antibody (ThermoFisher) for 1 h, a final washing step and the addition of TMB substrate (Sigma-Aldrich) according to the manufacturer's instructions. Absorbance at 450 nm was measured in an Infinite M200 Pro reader (Tecan). All steps were performed at room temperature and antiserum dilutions were measured in triplicate.

[0289] [Statistical analysis] All results are expressed as the mean + / - standard error of the mean (SEM). Statistical calculations were performed using GraphPad Prism software version 6. T-tests were used to compare two groups. For three or more groups, one-way ANOVA was used. Differences between groups were considered statistically significant at P<0.05.

[0290] Example 1: Sequential fragmentation of RNA encoding influenza nucleoprotein (NP) allows the identification and selection of small immunostimulatory RNA molecules (isRNA) with distinct TLR specificity and cytokine induction profiles Based on the finding that whole influenza A genomic RNA induced high levels of interferon alpha (IFN-α) in plasmacytoid dendritic cells (pDCs) upon Toll-like receptor 7 (TLR7) stimulation (Diebold SS. et al.; Science 303(5663), 1529-1531 (2004)), we selected RNA (1565 nt) encoding influenza A nucleoprotein (NP) as a parent sequence to establish a sequential fragmentation strategy to identify distinct small immunostimulatory single-stranded RNA molecules (isRNAs) responsible for TLR7-dependent IFN-α-inducing activity. In contrast to whole influenza A genomic RNA or total RNAs from other viruses, small isRNA molecules that induce distinct cytokine induction profiles and specific adaptive immune response modulation can be produced on a large scale under GMP conditions by chemical synthesis or in vitro transcription, enabling their clinical application as adjuvants for recombinant protein-based vaccines. Figure 1 shows an overview of the sequential fragmentation strategy applied using RNA encoding influenza NP (2-2-8, NP1-1565) as the starting sequence. Fragmentation was performed in iterative cycles, and selected fragments were transcribed into RNA in vitro, purified, and screened in vitro for their immunostimulatory activity and TLR specificity. Fragments with the highest TLR7 specificity, IFN-α inducing potential, and favorable cytokine induction profile were selected for further fragmentation cycles. The sequential fragmentation strategy finally identified and selected small isRNAs of 38-60 nucleotides in length, designated NP71-Seq4, Inno71-5A, NP71-Seq44, and NP71-Seq45.

[0291] Example 2: Synthesis of isRNA by in vitro transcription, composition and sequence of isRNA, and quality control after purification The DNA sequence encoding the selected influenza NP fragment was cloned into the plasmid pST1 using the SpeI and XhoI restriction sites downstream of the bacteriophage T7 RNA polymerase promoter. The XhoI-linearized plasmid served as the DNA template for the subsequent in vitro transcription (IVT). The IVT RNA synthesized by T7 RNA polymerase consisted of the selected influenza NP RNA fragment flanked on both sides by short stretches derived from the pST1 plasmid template (Figure 2A). The sequences of the final selected isRNAs NP71-Seq4, Inno71-5A, NP71-Seq44, and NP71-Seq45 and their overall sizes (in nucleotides, nt) including the pST1-derived sequence portion are shown in Figure 2B. The sequences derived from the pST1 template are underlined. The 5' end of the isRNAs is characterized by a triple guanosine residue, which represents the transcription initiation site for T7 RNA polymerase (Imburgio, D. et al.; Biochemistry 39(34), 10419-10430 (2000)). After purification on silica-based membranes or weak anion exchange columns, isRNA was quality-controlled by default using various analytical methods. The size, homogeneity, and integrity of purified isRNA were analyzed by denaturing 10% polyacrylamide (PAA) gel electrophoresis (Figure 2C) and on-chip capillary electrophoresis (Figure 2D) using a Bioanalyzer 2100 system (Agilent). Results are shown for isRNA NP71-Seq4, either produced by IVT or fully chemically synthesized (Chem.Synth.). NP71-Seq4 (IVT) was characterized by a clear single band of the expected size in the PAA gel and a sharp peak in capillary electrophoresis indicating a homogeneous population without any sign of RNA integrity loss. In contrast, chemically synthesized NP71-Seq4 isRNA revealed a peak shoulder and a second minor peak after capillary electrophoresis, pointing to a more heterogeneous RNA population. Therefore, IVT RNA was used in all subsequent experiments.

[0292] Example 3: Formulated isRNA induces high levels of IFN-α in human PBMCs Potential isRNA candidates derived by sequential fragmentation were screened for their immunostimulatory capacity in cellular in vitro assays after purification and quality control. Differential behavior of their ability to induce the derived cytokine IFN-α (strong induction) versus TNF-α (weak or absent induction) was defined as the first important criterion for candidate selection and further fragmentation steps. The isRNA-mediated induction of the potent proinflammatory cytokine TNF-α should be minimized as it may cause harmful systemic side effects and negatively affect the safety profile of the identified isRNA when used as a vaccine adjuvant. 1 x 10 per well 6Freshly isolated human PBMCs were stimulated with F5-formulated NP71 IVT RNA or fragments derived therefrom (NP71-Seq1-NP71-Seq4; see also Figure 1) and the levels of IFN-α and TNF-α secreted into cell culture supernatants upon stimulation were analyzed by ELISA. The isRNA test candidates were used at two different concentrations (0.167 and 0.041 μg / well) reflecting equivalent amounts or equivalent molar concentrations as the larger parental NP71 RNA fragments. F5-formulated poly(I:C) (0.167 μg / well) and unformulated CL097 (0.2 μg / well) were used as positive controls for IFN-α and TNF-α induction, respectively. Incubation of cells with medium or empty F5-liposomes served as negative controls. The immunostimulatory activity of the tested IVT RNAs depended on the appropriate delivery method, as shown in Figure 3A with liposomal formulation of NP71 RNA fragments (0.167 μg / well). The parental NP71 RNA fragment induced high levels of both IFN-α and TNF-α. In contrast to NP71, ELISA assays revealed that stimulation of human PBMCs with isRNA NP71-Seq4 resulted in a favorable cytokine induction profile with high IFN-α and low TNF-α amounts secreted in the cell supernatant. All other tested candidates caused a more balanced induction of both cytokines, especially evident when using high RNA concentrations (0.167 μg / well), ignoring further fragmentation steps. This result indicates that the applied sequential fragmentation strategy indeed allows to identify and select distinct RNA fragments with the desired immunostimulatory activity. NP71-Seq4 was selected for further fragmentation to identify shorter core sequence motifs responsible for the observed IFN-α induction effect. The resulting small isRNA fragments Inno71-5A, NP71-Seq44, and NP71-Seq45 (see also Figures 1 and 2B) were formulated with F5-liposomes and delivered at 1 × 10 per well. 6The Inno71-5A isRNA, which represents the shortest sequence stretch of NP71-Seq4, induced IFN-α levels comparable to its parent NP71-Seq4. This result indicates that Inno71-5A contains the key recognition sequence motif required for the observed immunostimulatory effect of NP71-Seq4. Unexpectedly, F5-formulated NP71-Seq44, and especially NP71-Seq45 isRNA, were able to induce even higher IFN-α levels than the parent NP71-Seq4. In contrast to Inno71-5A, which represents the 5′ sequence portion of NP71-Seq4, NP71-Seq44 and NP71-Seq45 contain the original 5′ and 3′ terminal sequences of NP71-Seq4 and were characterized by an internal depletion of the NP71-Seq4 RNA sequence portion (see Figure 1 ).

[0293] Example 4: pDCs can be targeted in vitro by F5-formulated isRNA and are the primary effector cells for IFN-α secretion upon stimulation with isRNA It is well known that human and mouse pDCs are the major cellular producers of IFN-α when stimulated with single-stranded (ss) viral RNA or ssRNA containing specific sequence motifs. To investigate whether F5-formulated small isRNA derived from influenza NP can target pDCs and activate IFN-α production, human pDCs were isolated from PBMCs using MACS technique and used in in vitro stimulation assays. Cells treated with empty F5-liposomes and medium alone served as negative controls. Unformulated CpG ODN2216 (5 μg / ml) served as a positive control for IFN-α induction (Figure 4). ELISA results showed that pDCs could be targeted by liposomally formulated isRNA and could produce high amounts of IFN-α upon stimulation. pDC-depleted PBMCs showed strongly reduced IFN-α induction compared to total PBMCs, indicating that pDCs are likely the major IFN-α producers targeted by liposomally formulated isRNA. Consistent with previous results using whole PBMCs, stimulation of pDCs with isRNA Inno71-5A, representing the shortest isRNA sequence, resulted in IFN-α levels very similar to those induced by the parental isRNA NP71-Seq4. Stimulation of pDCs with F5-formulated isRNA NP71-Seq45 induced higher amounts of IFN-α than NP71-Seq4, confirming previous experiments using whole PBMCs (see Figure 3B). Based on these results, isRNA NP71-Seq45 was selected as a lead candidate and was primarily used in further experiments.

[0294] Example 5: isRNA NP71-Seq45-mediated IFN-α induction in human PBMCs is dependent on endosome-located TLRs Induction of IFN-α in pDCs by whole influenza A genomic RNA is dependent on activation of TLR7 located in endosomes (Diebold SS. et al.; Science 303(5663), 1529-1531 (2004)). To assess the dependency of IFN-α induction upon stimulation with F5-formulated isRNA on nucleic acid-sensitive endosomal TLRs, freshly isolated total human PBMCs were pretreated with 250 nM bafilomycin A1 for 1 h and then incubated with NP71-Seq45 for 14 h (Figure 5). Bafilomycin A1 prevents endosomal acidification by inhibiting vacuolar H+ ATPase and blocks nucleic acid-sensitive TLRs located in endosomes such as TLR3, TLR7 / 8 or TLR9. Cells incubated with empty F5 liposomes and medium alone served as negative controls, while unformulated CpG ODN2216 (TLR9 ​​ligand) served as a positive control for bafilomycin A1 treatment. As expected, bafilomycin A1 pretreatment strongly reduced the induction of IFN-α by CpG ODN216, whereas cell viability was not affected by the bafilomycin A1 concentration used (data not shown). A similar strong reduction in IFN-α secretion was observed when PBMCs were incubated with bafilomycin A1 and continued with NP71-Seq45 stimulation, indicating that isRNA is recognized by endosomal TLRs.

[0295] Example 6: isRNA NP71-Seq45 is a specific agonist of TLR7 To analyze which nucleotide-sensitive endosomal TLRs are activated by NP71-Seq45, HEK293 cells stably co-expressing human TLR3, TLR7 or TLR8 in addition to an NF-κB-inducible luciferase reporter gene were incubated with F5-formulated isRNA NP71-Seq45 and luciferase signals were compared to untreated (medium only) cells (Figure 6). HEK293 cells expressing TLK9 were excluded because TLR9 exclusively recognizes specific DNA molecules. Positive controls were F5-formulated poly(I:C) for TLR3, unformulated CL097 for TLR7 and TLR8, and ssRNA40 complexed with cationic lipids for TLR8. Empty F5-liposome-treated cells served as negative controls. Addition of F5-formulated NP71-Seq45 isRNA activated the reporter gene only in HEK293 cells co-expressing human TLR7, but no luciferase signal was obtained in other TLR-expressing cells, indicating that NP71-Seq45 acts as a specific ligand for human TLR7. Taken together, these results demonstrate that liposomally formulated isRNA can induce IFN-α production in pDCs in vitro and that the immunostimulatory activity is mediated primarily by TLR7 located in endosomes.

[0296] Example 7: F5-formulated isRNA induces high levels of IFN-α but only low levels of IFN-γ, TNF-α, and IL10 in human PBMCs An important parameter for the selection of isRNA as a vaccine adjuvant was the ability to induce high levels of IFN-α and no or minimal levels of strong pro-inflammatory cytokines such as TNF-α and IFN-γ that may cause adverse systemic side effects upon vaccination. Furthermore, isRNA-based adjuvants should not result in strong induction of anti-inflammatory cytokines such as IL10, which inhibit the activity of Th1 cells, natural killer (NK) cells, and macrophages, thereby promoting a Th2 phenotype of the immune response. To analyze the cytokine profile induced by isRNA NP71-Seq45 and its parent fragment NP71-Seq4, a multiplex bead immunoassay was performed. 1 × 10 per well 6 Freshly isolated human PBMCs were incubated with F5-formulated isRNA (0.167 μg / well) or unformulated CpG ODN2216 (1 μg / well) as a positive control and empty F5-liposomes as a negative control. Analyzed cytokine concentrations in cell supernatants included IFN-α, IL6, IFN-γ, TNF-α, and IL10 (Figure 7). Both isRNAs induced high IFN-α levels, with NP71-Seq45 showing superior efficacy, confirming previous results. The amount of IFN-α detected was in a similar range to that observed for D-type CpG ODN2216, known to induce strong pDCIFN-α secretion. IL6, a cytokine with context-dependent pro- and anti-inflammatory properties and involved in B cell activation and maturation, was modestly induced by isRNA or CpG ODN2216, whereas only low levels of TNF-α, IFN-γ, and IL10 were detectable. The cytokine profile induced by isRNA in human PBMCs in vitro indicates that the identified isRNA initiates an innate immune response characterized by the production of Th1 cytokines, which has the potential to enhance the development of humoral and cellular antigen-specific immune responses while minimizing the induction of potentially harmful key proinflammatory cytokines.

[0297] Example 8: In vitro stimulation of mouse cells with F5-formulated isRNA results in strong pDC-dependent induction of IFN-α and only negligible TNF-α secretion Previous in vitro results using freshly prepared human PBMCs could clearly demonstrate the strong immunostimulatory ability of the identified isRNA, which showed high IFN-α induction and only slight TNF-α induction. In order to carry out meaningful in vivo preclinical studies using mouse models in subsequent experiments, the immunostimulatory effect had to be confirmed in vitro, including the responsible main target cells. Therefore, Balb / c bone marrow-derived cells and splenocytes were isolated and incubated with F5-formulated isRNA NP71-Seq4 or NP71-Seq45, unformulated CpG ODN2359 (0.5 μg / well), or empty F5 liposomes (Figure 8A). Untreated cells (cells only) served as negative controls. In addition, pDC-depleted splenocytes, enriched pDCs, and enriched common dendritic cells (cDCs) were stimulated as described above (Figure 8B). Cytokine concentrations in cell supernatants were analyzed by ELISA. Confirming the results obtained with human PBMCs, stimulation of mouse splenocytes or bone marrow-derived cells with isRNA resulted in potent IFN-α and only slight TNF-α induction. In contrast, incubation with the TLR9 agonist class C CpG ODN2359 induced a strong induction of both cytokines tested. Furthermore, human PBMC in vitro results (see also Figure 3B) revealed a stronger immunostimulatory potential of NP71-Seq45 than its parent RNA fragment NP71-Seq4. Induction of IFN-α by isRNA stimulation was strictly dependent on pDCs, as already observed using human cells. The in vitro results using freshly prepared mouse cells demonstrated the broad applicability of the identified isRNAs, enabling further in vivo experiments using mice as a model system.

[0298] Example 9: In vivo F12-formulated isRNA stimulation results in time- and dose-dependent induction of IFN-α The time and dose-response relationship when applying isRNA adjuvants are important parameters for safety evaluation and development of optimal immunization methods. Therefore, Balb / c mice were intravenously injected once with increasing doses of formulated NP71-Seq4 isRNA. In contrast to the liposomal formulation F5 used in the in vitro experiments, the liposomal formulation F12 used in the in vivo studies was adapted for optimal delivery of isRNA into the spleen, the main site of immune activation upon intravenous administration. Mouse serum samples were taken 1, 3, 5, 8 and 24 hours after injection to analyze the in vivo stimulatory effect of isRNA on the dose- and time-dependent induction of IFN-α measured by ELISA (Figure 9). Regardless of the administered dosage, maximum IFN-α induction in vivo was already achieved 5 h after immunization and then declined back to normal levels. Intravenous administration of 10 μg of NP71-Seq4 isRNA was already sufficient for a maximum stimulatory effect and could not be increased with higher doses. Even after injection of 40 μg of isRNA, no side effects were observed, indicating good tolerability of isRNA after systemic administration.

[0299] Example 10: Repeated intravenous administration of F12-formulated isRNA at frequent intervals results in systemic TLR response tolerance that can be overcome by adaptive immunization regimes TLR response tolerance is a well-known effect, where repeated exposure to TLR agonists reduces the cytokine release response (Broad, A. et al.; Curr Med Chem 13(21), 2487-2502 (2006)). As a consequence, vaccination with co-administration of isRNA adjuvants too frequently should be avoided, as it has a detrimental effect on the overall immune response. It was therefore important to analyze the time interval during which isRNA could be administered without leading to TLR response tolerance. F12-formulated NP71-Seq4 isRNA was injected intravenously twice into Balb / c mice with an interval of 5 days (first group) or 7 days (second group) (Figure 10). Both groups were injected with 20 μg of isRNA / mouse, and blood samples were collected 4 hours after each injection. The isRNA concentration used and the time of blood collection after injection were shown to be optimal in previous experiments (see Figure 9). Serum levels of IFN-α were detected by ELISA. Administration of F12-formulated NP71-Seq4 isRNA with a time interval of 5 days resulted in a pronounced TLR response tolerance effect accompanied by a strong decrease in IFN-α secretion after the second injection. However, TLR responsiveness could be almost completely restored with an injection interval of 7 days. Therefore, subsequent vaccination experiments combining model antigens with isRNA adjuvants were performed using multiple immunizations with a minimum time interval of 10-14 days after each injection to ensure maximum isRNA adjuvant effect.

[0300] Example 11: F12-formulated isRNA in combination with HBcAg-#A79 VLP induces antigen-specific B and T cell responses in vivo In previous experiments, we could demonstrate that the identified isRNA could stimulate the innate immune system, especially pDCs, very efficiently and significantly in a TLR7-dependent manner, releasing large amounts of IFN-α. In subsequent in vivo immune studies, we wanted to analyze whether triggering of the innate immune system by isRNA could be translated into an efficient and antigen-specific adaptive immune response of both B cells and T cells. For this purpose, we combined the selected isRNA with a virus-like particle (VLP)-based model antigen, HBcAg-#A79. VLPs are composed of viral structural proteins with an inherent ability to self-assemble into polymeric structures. VLPs resemble the viruses from which they are derived, but do not contain viral genetic material. Thus, VLPs are non-infectious and generally considered a safe vaccine type. The high intrinsic immunogenicity of VLPs can be transferred to heterologous epitopes repeatedly displayed on the surface of the VLPs. The model antigen used, HBcAg-#A79, is based on a recombinantly produced chimeric VLP derived from the Hepatitis B virus core antigen (HBcAg), which displays on its surface a genetically inserted epitope of the cell surface-located tumor-associated antigen (TAA) CLDN6. The CLDN6 epitope #A79 was an ideal candidate for immunization studies, since it serves as a composite epitope for B and T cells, the latter being restricted to the MHC class IH-2Kd expressed by Balb / c inbred mice. Balb / c mice were immunized intravenously four times at 2-week intervals with HBcAg-#A79 VLPs combined with F12-formulated isRNA NP71-Seq4 or NP71-Seq45. Naive mice or mice immunized with empty F12 liposomes, F12-formulated poly(A) RNA, or HBcAg-#A79-VLPs adjuvanted with CFA / IFA (immunized subcutaneously) served as controls. Ten days after the third immunization, blood samples were taken for analysis of antigen-specific humoral immune responses by flow cytometry (Figure 11A). Five days after the fourth immunization, animals were sacrificed and splenocytes were isolated to analyze the induction of specific T cell responses by enzyme-linked immunospot (ELISpot) assay (Figure 11B).

[0301] Flow cytometry analysis was performed using viable CHO-K1 cells stably transfected with the target TAA CLDN6 or its close homologue CLDN9 for specificity control. Detection of bound antibodies was expressed by the mean fluorescence intensity (MFI) shift calculated by dividing the MFI of immune serum by the MFI of each prebleed serum (before the first immunization). A MFI shift of more than 2-fold was considered as a positive antigen-specific antibody response. HBcAg-#A79 VLPs immunized without added adjuvant induce a weak antigen-specific immune response with only a small number of animals showing a positive reaction. The antigen-specific immune response was slightly increased by adjuvanting with poly(A)RNA, CFA / IFA, or empty F12 liposomes. However, this effect was not significant (ns) when compared to non-adjuvanted HBcAg-#A79VLPs. Coadministration of isRNA NP71-Seq4 or NP71-Seq45 strongly and highly significantly increased the CLDN6 antigen-specific antibody response upon immunization with HBcAg-#A79VLP without increasing nonspecific binding of antibodies to CLDN9. Immunization of animals with NP71-Seq45 isRNA as adjuvant generally resulted in a higher antibody MFI shift than NP71-Seq4. ELISpot analysis revealed that immunization with HBcAg-#A79VLPs adjuvanted with poly(A)RNA or CFA / IFA, and addition of empty F12 liposomes, completely lacked detectable T cell responses. In contrast, co-administration of isRNA induced highly significant #A79 peptide-specific T cell responses, with NP71-Seq4 increasing the number of IFN-γ spots when compared to NP71-Seq4. The results obtained by immunizing mice with HBcAg-#A79VLP in combination with liposomally formulated isRNA adjuvant clearly indicate that the efficient induction of high titer and antigen-specific antibody responses capable of detecting the target TAA in its native conformation on live cells was highly dependent on the isRNA adjuvant used. Moreover, only the combination of model antigen and formulated isRNA was able to simultaneously induce TAA peptide-specific T cell responses reflected by numerous IFN-γ spots. Thus, the identified isRNA administered intravenously in a liposomal formulation can act as an adjuvant to boost the development of humoral and cellular antigen-specific immune responses in vivo when combined with recombinant protein-based vaccines.

[0302] Example 12: Antigen-specific B and T cell responses induced by immunization with F12-formulated isRNA in combination with HBcAg-#A79 VLP are dose-dependent In previous experiments (see FIG. 9), we could show that the in vivo induction of IFN-α by formulated isRNA is time and dose dependent, with the maximum effect already achieved after a single injection of 10 μg of isRNA. The correlation of this dose dependency with the in vivo induction of antigen-specific combined B and T cell responses was analyzed by intravenously immunizing mice with increasing doses (5, 10, 20, 40 μg / injection) of HBcAg-#A79VLPs adjuvanted with F12-formulated NP71-Seq45 isRNA. The immunization schedule and experimental setup were identical to those described in Example 11. 5 μg / injection of F12-formulated NP71-Seq45 isRNA was already sufficient to show a clearly enhanced antibody response as shown by flow cytometry (FIG. 12A). In addition, a dose-dependent antigen-specific antibody response was detectable up to an isRNA concentration of 20 μg / injection without further increase when 40 μg / injection was applied. This dose-dependency was similar to the dose-dependent course of IFN-α secretion pointing to a key role of IFN-α in the adjuvant activity of F12-formulated isRNA. A significant enhancement of #A79 peptide-specific T cell response was detectable by IFN-γ ELISpot when at least 10 μg / injection of isRNA was applied (FIG. 12B). Moreover, in contrast to the results observed for isRNA-triggered B cell responses, increasing the isRNA adjuvant dosage up to 40 μg / injection of NP71-Seq45 further enhanced the peptide-specific T cell response. No nonspecific T cell reactivity against an irrelevant peptide derived from the TAA CLDN18.2 was observed even at the highest NP71-Seq45 dose. These results demonstrated the differential dose-dependent behavior of isRNA-induced B and T cell immune responses, allowing their modulation by adapting the applied isRNA dose.

[0303] Example 13: Antigen-specific antibodies induced by immunization with F12-formulated isRNA and HBcAg-#A79 VLP kill target-positive cells by CDC A successful vaccination strategy may depend on the induction of antigen-specific antibodies that can bind to their target proteins in their native conformation and subsequently mediate the killing of target-positive cells via immune effector mechanisms. One of the major cytolytic effector functions mediated by the Fc portion of antibodies is complement-dependent cytotoxicity (CDC), which was analyzed using a 1:10 dilution of unpurified serum from the immunization experiment described in Example 11. CDC assays revealed that sera from mice immunized with HBcAg-#A79VLP and F12-formulated isRNA exerted efficient cytolytic effector functions (Figure 13). Cytolytic activity was strictly dependent on active complement, as heat-inactivated complement (1:10hi) strongly reduced cell killing. Sera obtained from immunization with HBcAg-#A79VLP combined with empty F12 liposomes, CFA / IFA, or adjuvanted with F12-formulated poly(A)RNA were unable to exert comparable effector functions. The cytolytic effector functions of the induced antibodies largely correlated with their calculated MFI shifts. In mice, isotype IgG3 was the best complement activator, followed by IgG2a and IgG2b. Thus, the CDC assay results indicated that the isRNA adjuvant induced an in vivo cytokine environment that promoted immunoglobulin class switching, and the induced antigen-specific antibodies could kill target-positive cells by CDC.

[0304] Example 14: Immunization with F12-formulated isRNA in combination with HBcAg-#A79 VLP resulted in a balanced antigen-specific IgG2a / IgG1 response To better understand the adjuvant properties of the identified isRNAs in combination with the model antigen HBcAg-#A79VLP, the determination of immunoglobulin (Ig) isotype switching can provide insight into whether the vaccine composition affects the balance between the Th1 or Th2 profile of the immune response. In the murine system, the presence of elevated levels of IgG2a isotype is indicative of a Th1-mediated immune response, while high levels of IgG1 antibodies are characteristic of a Th2-mediated immune response. For the analysis of Ig isotype levels, sera from mice immunized as described in Example 11 were used to analyze titers of IgG1 or IgG2a antibodies specifically reacting against the linear #A79-peptide by ELISA (Figures 14A and 14B). Half-maximal antibody titers were calculated and expressed as the ratio of IgG2a to IgG1 (Figure 14C). Immunization with non-adjuvanted HBcAg-#A79VLPs or VLPs combined with empty F12 liposomes resulted in high IgG1 and very low IgG2a antibody titers, with a predominantly Th2-driven immune response. In contrast, antibodies raised by immunization with HBcAg-#A79VLPs adjuvanted with F12-formulated NP71-Seq45 induced moderately high IgG1 and strongly enhanced #A79-peptide-specific IgG2a antibody titers, resulting in a balanced Th1 / Th2 immune response.

[0305] Example 15: Antigen-specific antibody responses induced by F12-formulated isRNA in combination with HBcAg-#A79 VLP are primarily dependent on pDCs Previous in vitro experiments demonstrated that the identified isRNA exerted its immunostimulatory effect primarily through endosomal TLR activation in pDCs, resulting in the secretion of high amounts of IFN-α (see Figures 4 and 8). To confirm these results in vivo, BDAC2-DTR mice treated with diphtheria toxin (DT), which results in pDC depletion, and untreated C57BL / 6 wild-type mice were immunized intravenously three times at 14-day intervals with 50 μg / injection of HBcAg-#A79VLP combined with 20 μg / injection of F12-formulated NP71-Seq45 isRNA. Controls were C57BL / 6 wild-type mice immunized with HBcAg-#A79VLP alone or in combination with empty F12-liposomes, and BDAC2-DTR mice treated with DT alone but not antigen plus isRNA adjuvant. Serum samples were taken 4 hours after the first immunization to analyze induction of IFN-α by ELISA (Figure 15A) and 10 days after the last immunization to determine induced antigen-specific antibody responses by flow cytometry using CHO-K1 cells transfected with the target TAA CLDN6 or its close homologue CLDN9 (Figure 14B). pDC depletion by diphtheria toxin treatment strongly reduced IFN-α serum levels upon intravenous administration of F12-formulated NP71-Seq45 in combination with HBcAg-#A79VLP. Detectable levels were in a similar range to those observed for BDAC2-DTR mice treated with DT alone. Injection of unadjuvanted HBcAg-#A79VLP or HBcAg-#A79VLP adjuvanted with empty F12 liposomes did not result in detectable IFN-α serum levels. Analysis of CLDN6-specific antibodies by flow cytometry revealed that DT-mediated pDC depletion highly significantly reduced antigen-specific antibody responses upon immunization with F12-formulated NP71-Seq45isRNA in combination with HBcAg-#A79VLP compared to C67BL / 6 wild-type mice, however, antibody responses were still slightly elevated in BDAC2-DTR mice when compared to C57BL / 6 mice immunized with non-adjuvanted HBcAg-#A79VLP. The results showed that upon intravenous administration of formulated isRNA in vivo, pDCs were the primary targets and IFN-α producers, confirming previous in vitro studies. In addition, the lack of IFN-α induction significantly reduced antigen-specific humoral immune responses.

[0306] Example 16: F12-formulated isRNA stimulation results in substantial IFN-α induction and only slight TNF-α induction in vivo Previous in vitro data using mouse or human immune cells demonstrated the ability of the identified isRNAs, especially sequence NP71-Seq45, to induce high levels of type I interferon (IFN-α) and only slight levels of the strong proinflammatory cytokine TNF-α. To investigate whether formulated isRNAs could induce a similar cytokine profile in vivo, Balb / c mice were administered a single intravenous dose of F12-formulated NP71-Seq45 isRNA. Serum was then collected at various time points (4, 8, and 24 hours after injection) and IFN-α or TNF-α levels in serum were analyzed by commercial ELISA kits (Thermo Fischer). In agreement with the previously obtained in vitro data, we were able to clearly demonstrate that intravenous administration of F12-formulated NP71-Seq45 isRNA led to a very high but transient secretion of IFN-α, whereas TNF-α levels remained very low (Figure 16).

[0307] The sequences mentioned here are: SEQ ID NO:1 aacuucuggagggguga SEQ ID NO:2 aacuucuggaggggugaa u SEQ ID NO:3 uugcuu SEQ ID NO:4 aagaauugcu u SEQ ID NO:5 gggcgaacua guaacuucug gaggggugag aaucucga SEQ ID NO:6 aacuucuggaggggugauug cuu SEQ ID NO:7 gggcgaacua guaacuucug gaggggugau ugcuucucga SEQ ID NO:8 aacuucugga ggggugaa uggacgaaaa acaagaauug cuu SEQ ID NO:9 gggcgaacua guaacuucug gaggggugag aauggacgaa aaacaagaau ugcuucucga SEQ ID NO:10 aacuucugga ggggugagaa uaagaauugc uu SEQ ID NO:11 gggcgaacua guaacuucug gaggggugag aauaagaauu gcuucucga SEQ ID NO:12 agcaaaagca ggguagauaa ucacucacug agugacauca aaaucauggc gucucaaggc accaaacgau cuuacgaaca gauggagacu gauggagaac gccagaaugc cacugaaauc agagcauccg ucggaaaaau gauuggugga auuggacgau ucuacaucca aauuggcacc gaacucaaac ucagugauua ugagggacgg uugauccaaa acagcuuaac aauagagaga auggugcucu cugcuuuuga cgaaaggaga aauaaauacc uugaagaaca ucccagugcg gggaaagauc quaaghaaac uggaggaccu auauacagga gaguaaacgg aaaaggaug aggaacuca uccuuuauga siaagaaaaggcgaa ucuggcgcca agcuaaaau ggugacgaug caacggcugg cugacucac augaugaucu ggcauucca uuugaauugau gcaacuuauc shake aggcucucu cgcaccggaa shakeshagg gagugcucu cugaugcaag guucacucu cccuaggagg cugaggccg cugaggcugc aguchaaagg gugghaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa . ooooooooooooooooooooooooooarghaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaacaou cuckaaaaaaaaaaahaaaaaaaaaaahaaaaaaaaaaaaaaaaaaaaaaaaaaggggc cggaacccag ggaaugcuga guucgaagau cucacuuuuc uagcacgguc ugxacucaua uugagagggu cggugcuca caaguccugc cugcugccu guguguaugg accugccgua gccagugggu acgacuuuga aagggagg accucucuag acggaauaga cccuuucaga cugcucaa acagccaagu guacagccua aucagacca augagaaucc agcacacaag augcaacugg uguggauggc augccauucu gccgcauuug aagaucuaag aguauuaagc uucaucaaag ggacgaagg gcuccaaga ggacuagagg cover gcuuccaaug aaaauaugga gacuaugga ucaaguacac uugaacugag aagcagguac ugggccauaa ggaccagaag uggggaac accaaucaac agagggcauc ugcgggcca aucagcauac aaccuacguu cucaguacag agaaucucc cuuuugacag aacaaccguu auggcagcau ucaguggga uacagagggg agaacaucug acaugaggac cgaaucaua aggaugg aaagugcaag accagaagau gugucuuucc aggggcgggg aagucuucgag cucucggacg aaaaggcagc gagcccgauc cucucggacg uagacaugag uaaugaagga ucuuauuucu ucggagaca ucgauaauu aaagaaaaau acccuuguuu cuacu

Claims

1. A pharmaceutical composition for stimulating an immune response in a subject, comprising an immunostimulatory RNA molecule comprising a sequence selected from the group consisting of SEQ ID NOs: 2, and 5-11, at least one antigen, and a pharma- ceutically acceptable carrier, wherein the immunostimulatory RNA molecule has a length of 20 to 100 nucleotides and is formulated in a liposomal formulation.

2. The pharmaceutical composition of claim 1, wherein the immunostimulatory RNA molecule is capable of inducing an immune response, the immune response comprising (a) a B cell response, or (b) the production of IgG antibodies associated with a Th1-like response.

3. The pharmaceutical composition of claim 1 or 2, wherein the immunostimulatory RNA molecule is a Toll-like receptor (TLR) agonist.

4. The pharmaceutical composition of claim 3, wherein the TLR is TLR7 and the immunostimulatory RNA molecule comprises the sequence of SEQ ID NO: 6, 7, 10 or 11.

5. A pharmaceutical composition described in any one of claims 1 to 4, wherein the immunostimulatory RNA molecule is capable of inducing secretion of interferon alpha.

6. The pharmaceutical composition described in claim 5, wherein the secretion of interferon alpha is involved in plasmacytoid dendritic cells.

7. The pharmaceutical composition of claim 6, wherein the immunostimulatory RNA molecule does not substantially induce secretion of one or more of tumor necrosis factor alpha, interferon gamma, and interleukin 10.

8. 8. The pharmaceutical composition of claim 1, wherein the at least one antigen is selected from the group consisting of a cancer, viral, bacterial, fungal, or parasitic antigen.

9. A pharmaceutical composition described in any one of claims 1 to 8, wherein the subject is a mammal.

10. The pharmaceutical composition of claim 9, wherein the subject is a human.

11. An isolated RNA molecule comprising a sequence selected from the group consisting of SEQ ID NOs: 2, and 5-11, and having immunostimulatory activity, wherein the RNA molecule has a length of 20 to 100 nucleotides.

12. 12. The isolated RNA molecule of claim 11, which is a Toll-like receptor (TLR) agonist.

13. The isolated RNA molecule described in claim 12, wherein the TLR is TLR7 and has a sequence of SEQ ID NO: 6, 7, 10 or 11.

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