Diagnostic and therapeutic methods for the treatment of rheumatoid arthritis (RA)

Abstract

The present invention provides prognostic, predictive, and therapeutic methods for the treatment of rheumatoid arthritis (RA). The present invention is based, at least in part, on the discovery that the expression level of one or more biomarkers described herein in a sample (e.g., a synovial tissue sample, a synovial fluid sample, or a combination thereof) from an individual with RA can be used in methods for determining whether an individual with RA is likely to exhibit disease progression, identifying individuals with RA who are likely to respond to treatment including disease-modifying antirheumatic drugs (DMARDs), predicting the responsiveness of individuals with RA to treatment including DMARDs, selecting a therapy for an individual with RA, and treating individuals with RA, as well as related kits. [Selection diagram] None

Description

[Technical field]

[0001] Sequence Listing This application contains a Sequence Listing, which has been submitted electronically in ASCII format and is incorporated by reference in its entirety. The ASCII copy, created on January 22, 2019, is named 50474-175WO2_Sequence_Listing_1.22.19_ST25 and is 93,444 bytes in size.

[0002] The present invention is directed to diagnostic and therapeutic methods for the treatment of rheumatoid arthritis (RA). In particular, the present invention provides methods for prognosing disease progression and activity, predicting response to treatment, monitoring response to treatment, selecting a treatment, methods of treatment, and diagnostic kits. [Background technology]

[0003] Rheumatoid arthritis (RA) is an autoimmune disease of unknown etiology characterized by symmetric joint damage, inflammation, synovial lining hyperplasia, and the formation of invasive granulation tissue. If uncontrolled, the disease leads to eventual joint destruction and associated significant morbidity and increased mortality. The disease is heterogeneous with respect to its clinical and biological manifestations as well as its response to treatment.

[0004] RA is generally believed to be initiated by a breach of immune tolerance, particularly to citrullinated self-antigens, which may occur several years before the onset of clinical symptoms. Multiple cellular factors have been identified that contribute to the pathophysiology of RA, including the accumulation of autoreactive T and B cells in the synovium with the associated production of autoantibodies directed against various joint antigens, infiltration of inflammatory macrophages into the synovial lining and sublining, and increased production of cytokines and chemokines that play a role in recruiting, activating, and perpetuating synovitis.

[0005] Several therapeutic strategies, including disease-modifying antirheumatic drugs (DMARDs) such as methotrexate and leflunomide, and biological therapeutics targeting TNFα, IL-6, T cell co-stimulation, B cells, and Janus kinases, have shown high efficacy in managing RA symptoms and improving prognosis. However, the heterogeneity of RA makes it difficult to predict which individuals will benefit optimally from various therapies.

[0006] Thus, there is an unmet need for improved diagnostic and therapeutic methods for the treatment of individuals with RA. Summary of the Invention

[0007] The present invention provides diagnostic methods, therapeutic methods, and kits for treating individuals with rheumatoid arthritis (RA).

[0008] In a first aspect, the present invention deals with a method for predicting disease progression in an individual with RA, comprising determining the expression level of one or more genes listed in Table 1 in a sample from the individual, and a change in the expression level of one or more genes compared to a reference expression level identifies the individual as likely to exhibit disease progression. In certain embodiments, the change is an increase, and the one or more genes listed in Table 1 are selected from one or more genes listed in Table 2. In certain embodiments, the one or more genes listed in Table 2 include one or more of the following genes: CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A. In certain embodiments, the one or more genes listed in Table 2 include two or more of the following genes: CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A. In certain embodiments, the one or more genes listed in Table 2 include three or more of the following genes: CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A. In certain embodiments, the one or more genes listed in Table 2 include four or more of the following genes: CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A. In certain embodiments, the one or more genes listed in Table 2 include five or more of the following genes: CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A. In certain embodiments, the one or more genes listed in Table 2 include six or more of the following genes: CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A. In certain embodiments, the one or more genes listed in Table 2 include the following seven genes: CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A.In certain embodiments, the one or more genes listed in Table 2 consist of the following seven genes: CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A. In certain embodiments, the expression level of one or more genes listed in Table 2 is increased in the sample relative to a reference expression level, and the method further comprises administering to the individual a therapeutic agent other than or in addition to a disease-modifying antirheumatic drug (DMARD).

[0009] In other embodiments, the alteration is a decrease, and the one or more genes listed in Table 1 are selected from one or more genes listed in Table 3. In certain embodiments, the expression level of one or more genes listed in Table 3 is decreased in the sample relative to a reference expression level, and the method further comprises administering to the individual a therapeutic agent other than or in addition to a disease-modifying antirheumatic drug (DMARD).

[0010] In certain embodiments, disease progression is radiographic progression. In certain embodiments, radiographic progression is characterized by an increase in ShSS values ​​over a defined time frame.

[0011] In a second aspect, the present invention features a method of treating an individual with RA, the method comprising administering a therapeutic agent other than or in addition to a DMARD to the individual, the individual having been identified by an embodiment of the first aspect as likely to exhibit disease progression.

[0012] In a third aspect, the present invention features a method of treating an individual with RA, the method comprising: (a) obtaining a sample from the individual; (b) performing a gene expression assay on the sample to detect (i) an increased expression level of one or more genes listed in Table 2 and / or (ii) a decreased level of one or more genes listed in Table 3 in the sample compared to a reference expression level; (c) identifying the individual as having an increased likelihood of benefiting from a therapeutic agent other than or in addition to a DMARD; and (d) administering a therapeutic agent other than or in addition to a DMARD to the individual.

[0013] In a fourth aspect, the present invention features a method of treating an individual with RA, the individual being identified as having (i) an increased expression level of one or more genes listed in Table 2 in a sample from the individual, and / or (ii) a decreased expression level of one or more genes listed in Table 3 in a sample from the individual compared to a reference expression level, the method comprising administering to the individual a therapeutic agent other than or in addition to a DMARD.

[0014] In a fifth aspect, the present invention features a method of treating an individual with RA, the method comprising: (a) determining an expression level of one or more genes listed in Table 2 or Table 3 in a sample from the individual, wherein the expression level of one or more genes listed in Table 2 in the sample is determined to be increased and / or (ii) the expression level of one or more genes listed in Table 3 is determined to be decreased compared to a reference expression level; and (b) administering a therapeutic agent other than or in addition to a DMARD to the individual based on the expression level of one or more genes listed in Table 2 or Table 3 determined in step (a).

[0015] In certain embodiments of any one of the third, fourth, and fifth aspects, the one or more genes listed in Table 2 include one or more of the following genes: CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBAS3A.

[0016] In certain embodiments of any of the preceding aspects, the expression level of the one or more genes listed in Table 1 is an average of the expression levels of the one or more genes listed in Table 1. In certain embodiments, the average of the expression level of the one or more genes listed in Table 1 is an average of the normalized expression level of the one or more genes listed in Table 1.

[0017] In certain embodiments of any of the preceding aspects, the expression level of the one or more genes listed in Table 1 is a median expression level of the one or more genes listed in Table 1. In certain embodiments, the median expression level of the one or more genes listed in Table 1 is a median normalized expression level of the one or more genes listed in Table 1.

[0018] In certain embodiments of any of the preceding aspects, the normalized expression level of one or more genes listed in Table 1 is the expression level of one or more genes listed in Table 1 normalized to a reference gene. In certain embodiments, the reference gene is ACTB, GAPDH, GUSB, HPRT1, PGK1, RPL19, TUBB, TMEM55B, or a combination thereof.

[0019] In certain embodiments of any of the preceding aspects, the reference expression level is a pre-assigned expression level of one or more genes listed in Table 1. In certain embodiments, the reference expression level is an expression level of one or more genes listed in Table 1 in a reference population of individuals with RA who have not been previously treated with a DMARD, the reference population of individuals consisting of a first subset of individuals who have exhibited disease progression and a second subset of individuals who have not exhibited disease progression, and the reference expression level significantly separates the first and second subsets of individuals based on a significant difference between the expression level of one or more genes listed in Table 1 in the first subset of individuals compared to that of the second subset of individuals. In certain embodiments, after about 12 months, the first subset of individuals exhibited disease progression and the second subset of individuals did not exhibit disease progression.

[0020] In certain embodiments of the first or fifth aspect, the method further comprises determining one or more clinical covariates of the individual.

[0021] In certain embodiments of the second or fifth aspect, one or more clinical covariates have been determined for the individual.

[0022] In certain embodiments of any of the preceding aspects, the one or more clinical covariates are one or more of Disease Activity Score 28-Erythrocyte Sedimentation Rate (DAS28-ESR), Disease Activity Score 28-C-Reactive Protein (DAS28-CRP), Rheumatoid Factor (RF) titer, disease duration, baseline disease type, and ultrasound synovial thickening (USST) and ultrasound power Doppler (USPD) 12 maximum scores. In certain embodiments, the clinical covariate is DAS28-ESR. In certain embodiments, the clinical covariate is RF titer.

[0023] In certain embodiments of any of the preceding aspects, the expression level is the expression level of a nucleic acid. In certain embodiments, the expression level of a nucleic acid is the expression level of an mRNA. In certain embodiments, the expression level of an mRNA is determined by direct digital counting of nucleic acids, RNA-seq, RT-qPCR, qPCR, multiplex qPCR or RT-qPCR, microarray analysis, or a combination thereof. In certain embodiments, the direct digital counting of nucleic acids is by NANOSTRING® NCOUNTER® analysis.

[0024] In certain embodiments of any of the preceding aspects, the expression level is a protein expression level. In certain embodiments, the protein expression level is determined by immunoassay, liquid chromatography-mass spectrometry (LC-MS) technology, nephelometry, aptamer technology, or a combination thereof.

[0025] In a sixth aspect, the present invention features a method for identifying an individual with RA who may benefit from treatment comprising a DMARD, the method comprising determining a myeloid eigengene score from a sample from the individual, wherein a myeloid eigengene score from the sample that is equal to or greater than a reference myeloid eigengene score identifies the individual as one who may benefit from treatment comprising a DMARD.

[0026] In a seventh aspect, the present invention features a method of selecting a therapy for an individual with RA, the method comprising determining a myeloid intrinsic gene score from a sample from the individual, wherein a myeloid intrinsic gene score from the sample that is equal to or greater than a reference myeloid intrinsic gene score identifies the individual as one who may benefit from treatment including a DMARD.

[0027] In certain embodiments of the sixth or seventh aspect, the method further comprises determining a lymphoid intrinsic gene score from the sample from the individual, wherein a lymphoid intrinsic gene score that is equal to or greater than a reference lymphoid intrinsic gene score identifies the individual as one who may benefit from treatment including a DMARD.

[0028] In certain embodiments of the sixth or seventh aspect, the myeloid intrinsic gene score from the sample is equal to or greater than a reference myeloid intrinsic gene score, and the method further comprises administering to the individual a therapeutically effective amount of a DMARD.

[0029] In an eighth aspect, the present invention features a method of treating an individual with RA, the method comprising administering a DMARD to the individual, the individual having been identified by an embodiment of the sixth or seventh aspect as one who is likely to benefit from treatment comprising a DMARD.

[0030] In a ninth aspect, the present invention deals with a method for treating RA in an individual identified as having a myeloid-specific gene score from a sample from the individual that is equal to or greater than a reference myeloid-specific gene score, the method comprising administering a DMARD to the individual.In certain embodiments, before administration, the lymphoid-specific gene score from a sample from the individual is determined to be equal to or greater than a reference lymphoid-specific gene score.

[0031] In a tenth aspect, the present invention deals with a method for treating an individual with RA, the method comprises: (a) obtaining a sample from the individual; (b) carrying out a gene expression assay on the sample to determine that myeloid specific gene score is equal to or increased compared to a reference myeloid specific gene score; (c) identifying the individual as having an increased likelihood of benefiting from DMARDs; and (d) administering a DMARD to the individual.In certain embodiments, before identifying the individual as having an increased likelihood of benefiting from DMARDs, the method further comprises carrying out a gene expression assay on the sample to determine that lymphoid specific gene score is equal to or increased compared to a reference lymphoid specific gene score.

[0032] In an eleventh aspect, the present invention deals with a method for treating an individual with RA, the method comprising: (a) determining a myeloid-specific gene score from a sample from the individual, the myeloid-specific gene score from the sample being determined to be equal to or greater than a reference myeloid-specific gene score; and (b) administering a DMARD to the individual based on the myeloid-specific gene score determined in step (a).In certain embodiments, prior to administration, the method further comprises determining a lymphoid-specific gene score from the sample from the individual, the lymphoid-specific gene score in the sample being determined to be equal to or greater than a reference lymphoid-specific gene score.

[0033] In certain embodiments of any of the sixth, seventh, eighth, ninth, tenth, and eleventh aspects, the reference myeloid intrinsic gene score is from a reference population of individuals with RA who have been treated with DMARD therapy, the population of individuals consisting of a first subset of individuals who responded to DMARD therapy and a second subset of individuals who did not respond to DMARD therapy, and the reference myeloid intrinsic gene score significantly separates the first and second subsets of individuals based on a significant difference between the myeloid intrinsic gene score of the first subset of individuals compared to that of the second subset of individuals. In certain embodiments, about 6 months after the initiation of DMARD therapy, the first subset of individuals responded to DMARD therapy and the second subset did not respond to DMARD therapy.

[0034] In certain embodiments of any of the sixth, seventh, eighth, ninth, tenth, and eleventh aspects, the reference lymphoid-specific gene score is from a reference population of individuals with RA, the population of individuals consisting of a first subset of individuals that responded to DMARD therapy and a second subset of individuals that did not respond to DMARD therapy, and the reference lymphoid-specific gene score significantly separates the first and second subsets of individuals based on a significant difference between the lymphoid-specific gene score in the first subset of individuals compared to that of the second subset of individuals.

[0035] In certain embodiments of any of the sixth, seventh, eighth, ninth, tenth, and eleventh aspects, the individual has not been previously treated with a DMARD.

[0036] In certain embodiments of any of the sixth, seventh, eighth, ninth, tenth, and eleventh aspects, the individual has previously been treated with a DMARD.

[0037] In a twelfth aspect, the present invention features a method for monitoring the response of an individual with RA to treatment with a DMARD, the method comprising: (a) determining a first myeloid intrinsic gene score from a sample from the individual at a first time point during or after administration of a DMARD; (b) determining a second myeloid intrinsic gene score from a sample from the individual at a second time point; and (c) comparing the first myeloid intrinsic gene score to the second myeloid intrinsic gene score, wherein a decrease in the second myeloid intrinsic gene score compared to the first myeloid intrinsic gene score is predictive of the individual being likely to respond to treatment with a DMARD. In certain embodiments, the method further comprises: (a) determining a first lymphoid-specific gene score from a sample from the individual at a first time point during or after administration of a DMARD; (b) determining a second lymphoid-specific gene score from a sample from the individual at a second time point; and (c) comparing the first lymphoid-specific gene score with a second lymphoid-specific gene score, and a decrease in the second lymphoid-specific gene score compared to the first lymphoid-specific gene score is predictive of the individual being likely to respond to treatment with a DMARD.In certain embodiments, the second myeloid-specific gene score is decreased compared to the first myeloid-specific gene score, and the method further comprises administering an additional dose of a DMARD to the individual.In certain embodiments, the second lymphoid-specific gene score is decreased compared to the first lymphoid-specific gene score.

[0038] In certain embodiments of the twelfth aspect, the individual has previously been treated with a DMARD.

[0039] In certain embodiments of the twelfth aspect, the reduction is from about 1.25-fold to about 5-fold. In certain embodiments, the reduction is from about 1.25-fold to about 2-fold. In certain embodiments, the reduction is from about 1.25-fold to about 1.5-fold. In certain embodiments, the reduction is at least about 1.25-fold.

[0040] In certain embodiments of any of the preceding aspects, the sample is a synovial membrane sample. In certain embodiments, the synovial membrane sample is a synovial tissue sample or a synovial fluid sample.

[0041] In certain embodiments of any of the preceding aspects, the DMARD is methotrexate, hydroxychloroquine, sulfasalazine, leflunomide, azathioprine, cyclophosphamide, cyclosporine, mycophenolate mofetil, or a combination thereof.

[0042] In certain embodiments of any of the first, second, third, fourth, and fifth aspects, the therapeutic agent other than DMARD is a B cell antagonist, a Janus kinase (JAK) antagonist, a tumor necrosis factor (TNF) antagonist, a decoy TNF receptor, a T cell costimulatory signal antagonist, an IL-1 receptor antagonist, an IL-6 receptor antagonist, or a combination thereof.In certain embodiments, the JAK antagonist is tofacitinib.In certain embodiments, the IL-6 receptor antagonist is tocilizumab.In certain embodiments, the B cell antagonist is rituximab.

[0043] The application file contains at least one drawing executed in color. Copies of this patent or patent application containing color drawing(s) will be provided by the Office upon request and payment of the necessary fee. [Brief description of the drawings]

[0044] [Figure 1A] 1 is a table showing baseline demographic characteristics of a subset of 144 individuals in the PEAC cohort who were enrolled in the study. [Figure 1B] Representative images of ultrasound (US)-guided wrist biopsy. Inset, grayscale transverse US image of the wrist joint shows the biopsy needle entering the joint space below the extensor tendon complex. [Figure 1C] 1 is a graph showing the number and type of joints sampled. [Figure 2A] A series of representative images of histological specimens obtained from individuals recruited into the Pathobiology of Early Arthritis Cohort (PEAC) showing pathotype-specific immune cell infiltration (CD3+ T cells, CD68+ macrophages, CD20+ B cells, and CD138+ plasma cells) and H&E staining. All images are at 10x magnification. [Figure 2B] 1 is a table showing the mean levels of immune cell infiltration (CD3+ T cells, CD68+ macrophages, CD20+ B cells, and CD138+ plasma cells) by pathotype. Asterisks indicate significant differences between groups. [Figure 2C] Plot of concordance between NANOSTRING® and RNA-sequencing gene expression in the PEAC cohort data. Overall Spearman's rho = 0.85 between the two measurements. Individual patients are colored based on gene pathotype assignment (red = lymphoid, purple = myeloid, green = pauciimmune-fibroid, black = additional biology). [Figure 2D] 1 is a series of plots showing lymphoid (left panel), myeloid (middle panel), and microimmune-fibrotic (right panel) eigengene scores versus synovial aggregation scores (G1, G2, and G3 = grade 1, 2, and 3 aggregates, respectively). Asterisks represent statistical significance as determined by linear regression across groups: *=p<0.05, **=p<0.01, ***=p<0.001, and ns=non-significant. [Figure 3A]Heatmap of NANOSTRING® data. Raw log2 NANOSTRING® counts for 212 genes and 111 samples from individuals were normalized by probe to obtain a mean of 0 and a standard deviation of 1. Normalized data were clustered by rows and columns using Euclidean distance and Ward's linkage. Samples are colored according to IHC-determined pathotype, with ungraded samples colored gray. Rows are colored according to the pathotype the gene was originally associated with, with RA biology-related genes colored black. [Figure 3B] FIG. 1 is a series of plots showing intrinsic gene scores versus pathotype as determined by IHC. Individual intrinsic gene scores are plotted for each sample, grouped and colored by pathotype as determined by IHC. Asterisks represent statistical significance as determined by linear regression across groups: *=p<0.05, **=p<0.01, and ***=p<0.001. [Figure 3C] Figure 1 is a radar plot of normalized eigengene scores. Eigengene values ​​were normalized to obtain a mean of 0 and a standard deviation of 1. Samples were grouped by pathotype and the mean (solid line) and standard error of the mean (shaded area) were calculated for the normalized eigengenes. The spokes of the radar plot represent the distance along each normalized eigengene for each sample group. [Figure 3D] Volcano plot of gene expression differences across pathotypes. For each gene, a one-way ANOVA was performed to compare expression across the three pathotypes. The -log10p value from the one-way ANOVA is plotted against the root mean square of the log2 fold change between each pair of unique genes. Genes are colored according to the pathotype in which they were first identified, with RA biology-related genes colored black. [Figure 4A] 1 is a series of plots comparing eigengene scores, where the eigengene values ​​for the baseline samples are plotted against each other and colored according to the IHC-determined pathotype of the samples. [Figure 4B] A series of volcano plots showing comparisons between three pathotypes. Each pathotype was compared to the other two using linear regression. The log2 fold change and -log10 p-values ​​for these comparisons are shown, where genes are colored according to the pathotype to which they were originally assigned, with RA biology-related genes colored black. Genes that were significant at a Benjamini-Hochberg adjusted p-value <0.01 are shown as black dots, while those that do not meet this cutoff are shown as white dots. [Figure 5A] FIG. 1 is a table showing radiographic outcomes at 12 months for individuals stratified according to oligoimmune-fibrotic / myeloid versus lymphoid pathology. [Figure 5B] Volcano plot of pre-treatment genes differentially expressed in individuals with radiographic progression (ShSS>=1) at 1 year. P values ​​were from a two-sample t-test comparing progressors and non-progressors without adjustment. 46 genes with p-values ​​<0.05 are highlighted in red and fall above the dashed line. [Figure 5C] A series of plots showing baseline scores for lymphoid (left panel), myeloid (middle panel), and microimmune-fibrotic (right panel) versus radiographic progression status (ΔShSS≧1) at 1 year. **=p<0.01 by t-test. [Figure 5D] Gene set enrichment plot showing higher expression of osteoclast-specific genes in lymphoid versus fibrous / myeloid pathotypes. The x-axis shows the log2 fold change between lymphoid and fibrous / myeloid samples. The density shown in grey indicates the distribution of fold change across all genes assayed. Osteoclast-specific genes are shown as black dots and the overall distribution of fold change is shown as a black line. [Figure 5E]Figure 1 shows ROC plots showing identification of clinical and gene expression features predictive of radiographic progression at 1 year. The 46 genes and selected covariates as listed in Table 1 (8 clinical covariates, i.e. baseline RF titer, disease duration, VAS, swollen joint count, DAS28-ESR, baseline disease type, and 12 maximum scores of ultrasound synovial thickening (USST) and ultrasound power Doppler (USPD)) were simultaneously entered into a logistic model with L1 regularization penalty (LASSO) to determine the optimal sparse prediction model. The model shows improved predictive performance with penalized clinical covariates (blue dashed line) compared to unpenalized clinical covariates (red dotted line), and both of these models outperform clinical measures alone (black line). [Figure 6A] Lambda training curve from the final glmnet fitted model. The red dots represent the mean binomial deviance using 10-fold cross-validation. The error bars represent the standard error of the binomial deviance. The vertical dotted lines indicate the minimum binomial deviance (λmin) and a more regularized model where the error of the binomial deviance is within one standard error of the minimum binomial deviance (λ1stErr). We chose λmin, which corresponds to 9 nonzero coefficients in the final model. [Figure 6B] 13 is a table showing non-zero coefficients associated with the final variables selected by the LASSO regression. [Figure 7A] FIG. 1 is a table showing baseline clinical and histological parameters stratified according to the three pathological subtypes (n=129). Asterisks indicate significant differences. [Figure 7B] 1 is a chart showing correlation analysis of each eigengene score with clinical disease activity, autoantibodies, acute phase reactants, and ultrasound metrics. Values ​​represent Spearman correlation coefficients between clinical variables and individual eigengene scores. Asterisks represent the significance of correlation coefficients: *=p<0.05, **=p<0.01, and ***=p<0.001. [Figure 8A]FIG. 1 is a table showing clinical changes in disease activity by pathotype (lymphoid, myeloid, and oligoimmune-fibrotic) and treatment regimens according to pathotype. [Figure 8B] Volcano plot showing gene expression change between baseline and 6 months in EULAR-responsive individuals. Individual points are colored by the pathotype in which the gene was originally identified, with RA biology-related genes colored black. [Figure 8C] Volcano plot showing gene expression change between baseline and 6 months in EULAR non-responders. Individual points are colored by the pathotype in which the gene was originally identified, with RA biology-related genes colored black. [Figure 8D] FIG. 1 is a series of plots showing the correlation between pre-treatment lymphoid (left panel), myeloid (middle panel), and microimmuno-fibrotic (right panel) eigengene scores and the change in DAS28-ESR after 6 months of DMARD treatment. Spearman's correlation coefficient is shown along with the significance of this value. [Figure 8E] A series of pairwise plots of lymphoid (left panel), myeloid (middle panel), and microimmune-fibrotic (right panel) eigengene scores at baseline and 6 months in individuals who achieved good or poor clinical response to DMARD treatment at 6 months by EULAR response criteria. Individuals who achieved a good response or failed to achieve a moderate response according to EULAR criteria are shown. For each individual, the pre-treatment eigengene score is connected with the post-treatment eigengene score for each of the three eigengenes. Asterisks represent the significance of the difference between pre-treatment and post-treatment samples using a linear mixed-effects model with sample date as a fixed effect and individual as a random effect: *=p<0.05, **=p<0.01, and ***=p<0.001. [Figure 9A]1 is a chart showing the correlation of pre-treatment serum CXCL13, sICAM1, MMP3, and IL-8 with clinical disease metrics and ultrasound scores. Values ​​represent Spearman correlation coefficients between clinical variables and individual eigengene scores. Asterisks represent the significance of correlation coefficients: *=p<0.05, **=p<0.01, and ***=p<0.001. [Figure 9B] 1 is a chart showing the correlation of pre-treatment serum CXCL13, sICAM1, MMP3, and IL-8 with synovial histology scores. Values ​​represent Spearman correlation coefficients between clinical variables and individual eigengene scores. Asterisks represent the significance of correlation coefficients: *=p<0.05, **=p<0.01, and ***=p<0.001. [Figure 9C] FIG. 1 is a plot of serum CXCL13 concentration versus synovial pathotype status, showing elevated levels in lymphoid versus myeloid or oligoimmune-fibrotic pathotypes. P values ​​were calculated using a t-test. [Figure 9D] FIG. 1 is a plot of serum MMP3 concentration versus synovial pathotype status, showing elevated levels in lymphoid versus myeloid or oligoimmune-fibrotic pathotypes. P values ​​were calculated using t-test. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0045] I. Definition It is to be understood that aspects and embodiments of the invention described herein include "comprising," "consisting," and "consisting essentially of" aspects and embodiments. As used herein, the singular forms "a," "an," and "the" include plural referents unless otherwise indicated.

[0046] The term "about" as used herein refers to a normal error range for each corresponding value, which is readily apparent to those skilled in the art. Reference herein to "about" a value or parameter includes (and describes) an embodiment that is directed to the value or parameter itself. For example, a description that refers to "about X" includes a description of "X".

[0047] The term "pathotype" as used herein refers to a subtype of RA characterized by certain pathological, histological, and / or clinical features of RA. The pathological, histological, and / or clinical features of RA may be further associated with the expression of one or more specific genes or one or more specific proteins, or a particular expression pattern of a combination of genes or a combination of proteins. Such pathotypes include, but are not limited to, lymphocytic pathotypes (e.g., characterized by B cell-rich aggregates), myeloid pathotypes (e.g., characterized by predominant macrophage infiltration), and oligoimmune-fibrotic pathotypes (e.g., characterized by a low number of infiltrating immune cells, but still with proliferation of fibroblast lineage cells in the sublining and lining). In some embodiments, the pathotype is lymphocytic pathotype and further characterized by expression of one or more genes listed in Table 9. In some embodiments, the pathotype is myeloid pathotype and further characterized by expression of one or more genes listed in Table 10. In some embodiments, the pathotype is a minimal immune-fibrotic pathotype and is further characterized by expression of one or more genes listed in Table 11.

[0048] The term "biomarker" as used herein refers to an indicator, e.g., a predictive and / or prognostic indicator, that can be detected in a sample (e.g., a gene) or can be derived from one or more indicators detected from a sample (e.g., an intrinsic gene score). A biomarker can serve as an indicator of a particular subtype of a disease or disorder (e.g., rheumatoid arthritis) characterized by certain molecular, pathological, histological, and / or clinical features. In some embodiments, a biomarker is a gene. In other embodiments, a biomarker is a collection of genes. Biomarkers include, but are not limited to, polynucleotide (e.g., DNA and / or RNA), changes in polynucleotide copy number (e.g., DNA copy number), polypeptide, polypeptide and polynucleotide modifications (e.g., post-translational modifications), carbohydrate, and / or glycolipid-based molecular markers.

[0049] Such biomarkers include, but are not limited to, CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, UBASH3A, BLK, BTK, BTLA, CCL19, CD19, CD38, CD40LG, CILP, DENND2D, DKK3, FCRL5, FGF2, FGF9, HLA-DOB, ICOS, JCHAIN, IL36B, IRF4, LOC100505746, LY9, MAP4K1, MMP1, NOG, PIM2, POU2AF1, RHOH, SEL1L3, SIRPG, SLAMF6, SLC31A1, SPIB, TIGIT, TLR10, TMC6, TNF, TNFRSF11B, TNFRSF17, TRAF3PIP3, and XBP1, or combinations thereof. In some embodiments, the biomarker is one or more biomarkers selected from the group consisting of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A. In other embodiments, the biomarker is one or more biomarkers selected from the group consisting of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, UBASH3A, BLK, BTK, BTLA, CCL19, CD19, CD38, CD40LG, DENND2D, FCRL5, HLA-DOB, ICOS, JCHAIN, IL36B, IRF4, LOC100505746, LY9, MAP4K1, MMP1, PIM2, POU2AF1, RHOH, SEL1L3, SIRPG, SLAMF6, SLC31A1, SPIB, TIGIT, TLR10, TMC6, TNF, TNFRSF17, TRAF3PIP3, and XBP1. In still other embodiments, the biomarker is one or more biomarkers selected from the group consisting of CILP, DKK3, FGF2, FGF9, NOG, and TNFRSF11B.Expression of such biomarkers may be determined to be higher or lower in samples obtained from patients who are sensitive to or responsive to treatment (e.g., treatment with rheumatoid arthritis therapy, including DMARD therapy) than a reference level, including, for example, the median expression level of the biomarker in samples from a group / population of patients, e.g., patients who have RA and are being tested for responsiveness to treatment; the median expression level of the biomarker in samples from a group / population of patients, e.g., patients who have RA and have been identified as not responding to treatment; levels in a sample previously obtained from the individual at a prior time point; or levels in samples from patients who have undergone prior treatment (e.g., treatment with a DMARD).

[0050] The term "CD180" as used herein refers to any native CD180 antigen from any vertebrate source, including mammals such as primates (e.g., humans) and rodents (e.g., mice and rats), unless otherwise indicated. The term encompasses "full-length" unprocessed CD180 as well as any form of CD180 resulting from processing in cells. The term also encompasses naturally occurring variants of CD180, such as splice variants or allelic variants. CD180 is also referred to in the art as lymphocyte antigen 64 (LY64), radioprotective 105 kDa protein (RP105), and Ly78. An exemplary nucleic acid sequence of human CD180 is shown under NCBI Reference Sequence: NM_005582.2 or in SEQ ID NO:1. An exemplary amino acid sequence of a protein encoded by human CD180 is shown under UniProt Accession No. Q99467 or in SEQ ID NO:2.

[0051] The term "CSF2" as used herein refers to any native granulocyte-macrophage colony-stimulating factor from any vertebrate source, including mammals such as primates (e.g., humans) and rodents (e.g., mice and rats), unless otherwise indicated. The term encompasses "full-length" unprocessed CSF2 as well as any form of CSF2 resulting from processing in cells. The term also encompasses naturally occurring variants of CSF2, such as splice variants or allelic variants. CSF2 is also referred to in the art as GM-CSF, colony-stimulating factor, CSF, molgramostin, and sargramostim. An exemplary nucleic acid sequence of human CSF2 is set forth under NCBI Reference Sequence: NM_000758.3, or in SEQ ID NO: 3. An exemplary amino acid sequence of a protein encoded by human CSF2 is set forth under UniProt Accession No. P04141, or in SEQ ID NO: 4.

[0052] The term "CXCL1" as used herein refers to any native CXC motif chemokine 1 from any vertebrate source, including mammals such as primates (e.g., humans) and rodents (e.g., mice and rats), unless otherwise indicated. The term encompasses "full-length" unprocessed CXCL1 as well as any form of CXCL1 resulting from processing in cells. The term also encompasses naturally occurring variants of CXCL1, such as splice variants or allelic variants. CXCL1 is also referred to in the art as chemokine ligand 1, growth regulatory alpha protein, GRO-alpha(1-73), melanoma growth stimulating activity, MGSA, neutrophil activating protein 3, and NAP-3. An exemplary nucleic acid sequence of human CXCL1 is shown under NCBI Reference Sequence: NM_001511.3, or in SEQ ID NO:5. The amino acid sequence of an exemplary protein encoded by human CXCL1 is shown under UniProt accession number P09341, or in SEQ ID NO:6.

[0053] The term "DENND1C" as used herein refers to any native DENN domain-containing protein 1C from any vertebrate source, including mammals such as primates (e.g., humans) and rodents (e.g., mice and rats), unless otherwise indicated. The term encompasses "full-length" unprocessed DENND1C as well as any form of DENND1C resulting from processing in cells. The term also encompasses naturally occurring variants of DENND1C, such as splice variants or allelic variants. DENND1C is also referred to in the art as connecte3 and protein FAM31C. An exemplary nucleic acid sequence of human DENND1C is shown under NCBI reference sequence: NM_001290331.1, or in SEQ ID NO: 7. An exemplary amino acid sequence of a protein encoded by human DENND1C is shown under UniProt accession number Q8IV53, or in SEQ ID NO: 8.

[0054] The term "MMP10" as used herein refers to any native matrix metallopeptidase 10 from any vertebrate source, including mammals such as primates (e.g., humans) and rodents (e.g., mice and rats), unless otherwise indicated. The term encompasses "full-length" unprocessed MMP10 as well as any form of MMP10 resulting from processing in cells. The term also encompasses naturally occurring variants of MMP10, such as splice variants or allelic variants. MMP10 is also referred to in the art as stromelysin-2, SL-2, and transin-2. An exemplary nucleic acid sequence of human MMP10 is set forth under NCBI Reference Sequence: NM_002425.2, or in SEQ ID NO: 9. An exemplary amino acid sequence of a protein encoded by human MMP10 is set forth under UniProt Accession No. P09238, or in SEQ ID NO: 10.

[0055] The term "SDC1" as used herein refers to any native syndecan1 from any vertebrate source, including mammals such as primates (e.g., humans) and rodents (e.g., mice and rats), unless otherwise indicated. The term encompasses "full-length" unprocessed SDC1 as well as any form of SDC1 resulting from processing in cells. The term also encompasses naturally occurring variants of SDC1, such as splice variants or allelic variants. SDC1 is also referred to in the art as SYND1 and CD138. An exemplary nucleic acid sequence of human SDC1 is set forth under NCBI Reference Sequence: NM_001006946.1, or in SEQ ID NO: 11. An exemplary amino acid sequence of a protein encoded by human SDC1 is set forth under UniProt Accession No. P18827, or in SEQ ID NO: 12.

[0056] The term "UBASH3A" as used herein refers to any native ubiquitin associated and SH3 domain containing protein A from any vertebrate source, including mammals such as primates (e.g., humans) and rodents (e.g., mice and rats), unless otherwise indicated. The term encompasses "full-length" unprocessed UBASH3A as any form of UBASH3A from processing in a cell. The term also encompasses naturally occurring variants of UBASH3A, such as splice variants or allelic variants. UBASH3A is also referred to in the art as Cbl interacting protein 4, CLIP4, suppressor of T cell receptor signaling 2, STS-2, T cell ubiquitin ligand 1, and TULA-1. An exemplary nucleic acid sequence of human UBASH3A is shown under NCBI Reference Sequence: NM_001001895.2, or in SEQ ID NO: 13. The amino acid sequence of an exemplary protein encoded by human UBASH3A is shown under UniProt accession number P57075, or in SEQ ID NO:14.

[0057] As used herein, the terms "myeloid intrinsic gene score" and "myeloid intrinsic gene" (each of which may be used interchangeably) refer to a numerical value that reflects the degree or amount of pathological, histological, and / or clinical features of myeloid pathology RA and correlates with the expression level of one or more genes set forth in Table 10 detected in a sample (e.g., a synovial tissue sample, a synovial fluid sample, or a combination thereof) obtained from an individual (e.g., an individual with RA). The myeloid intrinsic gene score may serve as a biomarker (e.g., a predictive and / or prognostic biomarker) for myeloid pathology RA.

[0058] As used herein, the terms "lymphoid-specific gene score" and "lymphoid-specific genes" (each of which may be used interchangeably) refer to a numerical value that reflects the degree or amount of pathological, histological, and / or clinical features of RA of the lymphoid phenotype and correlates with the expression level of one or more genes set forth in Table 9 detected in a sample (e.g., a synovial tissue sample, a synovial fluid sample, or a combination thereof) obtained from an individual (e.g., an individual with RA). The lymphoid-specific gene score may serve as a biomarker (e.g., a predictive and / or prognostic biomarker) for RA of the lymphoid phenotype.

[0059] As used herein, the terms "minor immune-fibrotic intrinsic gene score" and "minor immune-fibrotic intrinsic gene" (each of which may be used interchangeably) refer to a numerical value that reflects the degree or amount of pathological, histological, and / or clinical features of minor immune-fibrotic pathology of RA and correlates with the expression level of one or more genes set forth in Table 11 detected in a sample (e.g., a synovial tissue sample, a synovial fluid sample, or a combination thereof) obtained from an individual (e.g., an individual having RA). The minor immune-fibrotic intrinsic gene score may serve as a biomarker (e.g., a predictive and / or prognostic biomarker) for minor immune-fibrotic pathology of RA.

[0060] The term "detecting" is used in the broadest sense herein to include both qualitative and quantitative measurements of a target molecule. Detecting includes identifying the mere presence of a target molecule in a sample, as well as determining whether the target molecule is present in a sample at detectable levels. Detecting may be direct or indirect.

[0061] The terms "level of expression" or "expression level" are generally used interchangeably and generally refer to the amount of a biomarker in a biological sample. "Expression" generally refers to the process by which information (e.g., genetic coding information and / or epigenetic information) is converted into structures present and operative in a cell. Thus, as used herein, "expression" can refer to transcription into a polynucleotide, translation into a polypeptide, or even polynucleotide and / or polypeptide modification (e.g., post-translational modification of a polypeptide). Fragments of transcribed polynucleotides, translated polypeptides, or polynucleotide and / or polypeptide modifications (e.g., post-translational modification of a polypeptide) shall also be considered expressed, regardless of whether they are derived from transcripts generated by alternative splicing or degraded transcripts, or from post-translational processing of a polypeptide, e.g., by proteolysis. "Expressed genes" include those that are transcribed into polynucleotides as mRNA and then translated into a polypeptide, and also those that are transcribed into RNA but not translated into a polypeptide (e.g., transfer RNA and ribosomal RNA). The expression level of more than one gene of interest may be determined by counting methods known to those skilled in the art and also disclosed herein, including, for example, calculating the median or average of all expression levels of the genes of interest.Before counting, the expression level of each gene of interest may be normalized by using statistical methods known to those skilled in the art and also disclosed herein, including, for example, normalizing to the expression level of one or more housekeeping genes, or normalizing to the total library size, or normalizing to the median or average expression level across all genes measured.In some cases, before counting across multiple genes of interest, the normalized expression level of each gene of interest may be standardized by using statistical methods known to those skilled in the art and also disclosed herein, including, for example, calculating the Z-score of the normalized expression level of each gene of interest.

[0062] The term "sample" as used herein refers to a composition obtained or derived from a patient and / or individual of interest that contains cellular and / or other molecular entities to be characterized and / or identified, for example, based on physical, biochemical, chemical, and / or physiological properties. Samples include, but are not limited to, synovial tissue samples, synovial fluid samples, tissue samples, primary or cultured cells or cell lines, cell supernatants, cell lysates, platelets, serum, plasma, vitreous fluid, lymphatic fluid, follicular fluid, semen, amniotic fluid, milk, whole blood, cells from blood, urine, cerebrospinal fluid, saliva, sputum, tears, sweat, mucus, tumor lysates, and tissue culture media, tissue extracts, e.g., homogenized tissue, tumor tissue, cell extracts, and combinations thereof.

[0063] A sample or cell that "expresses" a protein of interest is one in which the mRNA encoding that protein, or that protein (including fragments thereof) is determined to be present in that sample or cell.

[0064] As used herein, the terms "reference expression level" and "reference level" are used interchangeably to refer to an expression level against which another expression level, e.g., the expression level of one or more genes described herein (e.g., any gene or combination of genes described in any one of Tables 1-3) in a sample from an individual, is compared, e.g., to make a diagnostic (e.g., predictive and / or prognostic) and / or therapeutic decision. For example, the reference expression level may be derived from an expression level in a reference population (e.g., a median expression level in a reference population, e.g., a population of patients with RA who have not been treated with a RA therapeutic agent (e.g., a DMARD (e.g., methotrexate, hydroxychloroquine, sulfasalazine, leflunomide, azathioprine, cyclophosphamide, cyclosporine, or mycophenolate mofetil)), an expression level in a reference sample, and / or a pre-assigned value (e.g., a cut-off value that is pre-determined to significantly (e.g., statistically significantly) separate a first subset of individuals who have exhibited disease progression from a second subset of individuals who have not exhibited disease progression), where the reference expression level significantly separates the first and second subsets of individuals based on a significant difference between the expression level in the first subset of individuals compared to that of the second subset of individuals. In some embodiments, the cut-off value may be a median or mean expression level in the reference population. In other embodiments, the reference level may be the top 40%, top 30%, top 20%, top 10%, top 5%, or top 1% of the expression levels in a reference population. In certain embodiments, the cutoff value may be the median expression level in a reference population. Those skilled in the art will understand that the value of the reference expression level may vary depending on the indication or disorder (e.g., RA), the technique used to detect the expression level (e.g., RNA-seq, microarray analysis, or RT-qPCR), and / or the particular combination of genes tested (e.g., any combination of genes listed in Tables 1-3).

[0065] As used herein, the terms "reference myeloid intrinsic gene score" and "reference myeloid intrinsic gene" are used interchangeably to refer to a myeloid intrinsic gene score against which another myeloid intrinsic gene score is compared, e.g., to make a diagnostic (e.g., predictive and / or prognostic) and / or therapeutic decision. For example, a reference myeloid intrinsic gene score can be a myeloid intrinsic gene score in a reference sample, a reference population, and / or a pre-defined value. In some cases, the reference myeloid intrinsic gene score is a cutoff value that significantly separates a first subset of individuals in a reference population who have been treated with a RA therapeutic agent (e.g., a DMARD therapeutic agent, e.g., a therapeutic agent that includes methotrexate) from a second subset of individuals in the same reference population who have been treated with a RA therapeutic agent (e.g., a DMARD therapeutic agent, e.g., a therapeutic agent that includes methotrexate) based on a significant difference between the responsiveness of the individuals to treatment with the RA therapeutic agent, where the cutoff value significantly separates the first subset of individuals who responded to the RA therapeutic agent from the second subset of individuals who did not respond to the RA therapeutic agent. In some cases, the cutoff value may be the median myeloid intrinsic gene score or the average myeloid intrinsic gene score in the reference population. In other cases, the reference myeloid intrinsic gene score may be the top 40%, top 30%, top 20%, top 10%, top 5%, or top 1% of the myeloid intrinsic gene score values ​​in the reference population. One of skill in the art will understand that the value of the reference myeloid-specific gene score may vary depending on the technique used to detect the expression levels of the genes that comprise the score (e.g., direct digital counting of nucleic acids (e.g., NANOSTRING® NCOUNTER®), RNA-seq, microarray analysis, or RT-qPCR) and / or the particular combination of genes tested to derive the reference myeloid-specific gene score (e.g., any combination of genes listed in Table 10).

[0066] As used herein, the terms "reference lymphoid-specific gene score" and "reference lymphoid-specific gene" are used interchangeably to refer to a lymphoid-specific gene score against which another lymphoid-specific gene score is compared, e.g., to make a diagnostic (e.g., predictive and / or prognostic) and / or therapeutic decision. For example, a reference lymphoid-specific gene score can be a lymphoid-specific gene score in a reference sample, a reference population, and / or a pre-defined value. In some cases, the reference lymphoid-specific gene score is a cutoff value that significantly separates a first subset of individuals in a reference population that have been treated with an RA therapeutic agent (e.g., a DMARD therapeutic agent, e.g., a therapeutic agent that includes methotrexate) from a second subset of individuals in the same reference population that have been treated with an RA therapeutic agent (e.g., a DMARD therapeutic agent, e.g., a therapeutic agent that includes methotrexate) based on a significant difference between the responsiveness of the individuals to treatment with the RA therapeutic agent, where the cutoff value significantly separates the first subset of individuals that responded to the RA therapeutic agent from the second subset of individuals that did not respond to the RA therapeutic agent. In some cases, the cutoff value may be the median lymphoid-specific gene score or the average lymphoid-specific gene score in the reference population. In other cases, the reference lymphoid-specific gene score may be the top 40%, top 30%, top 20%, top 10%, top 5%, or top 1% of the lymphoid-specific gene score values ​​in the reference population. One of skill in the art will understand that the value of the reference lymphoid-specific gene score may vary depending on the technique used to detect the expression levels of the genes that comprise the score (e.g., direct digital counting of nucleic acids (e.g., NANOSTRING® NCOUNTER®), RNA-seq, microarray analysis, or RT-qPCR) and / or the particular combination of genes tested to derive the reference lymphoid-specific gene score (e.g., any combination of genes listed in Table 9).

[0067] Expression "above a level" (e.g., above a reference level), "increased," "increased expression," "increased expression level," "increased level," "elevated," "elevated expression," "elevated expression level," or "elevated level" refers to increased expression of a biomarker, increased level of a biomarker, or increased eigengene score in an individual compared to the expression level or eigengene score of a biomarker in a control (e.g., an individual or individuals not afflicted with a disease or disorder (e.g., RA), an internal control (e.g., a housekeeping biomarker), the level of the biomarker or eigengene score in a sample obtained from an individual prior to administration of a therapeutic agent (e.g., a DMARD)) or compared to a reference level (e.g., a patient, e.g., the median expression level or median eigengene score of a biomarker in samples from a group / population of patients with RA who are being tested for responsiveness to RA therapeutic agents, including DMARDs; a median expression level or median eigengene score of a biomarker in samples from a group / population of patients, e.g., patients with RA identified as not responsive to DMARDs; or a level in a sample previously obtained from the individual at a prior time point).

[0068] Expression "below" a level (e.g., below a reference level), "reduced," "reduced expression," "reduced expression level," "reduced level," "reduced," "reduced expression," "reduced expression level," or "reduced level" refers to the expression level or intrinsic gene score of a biomarker in a control (e.g., an individual or individuals not afflicted with a disease or disorder (e.g., RA), an internal control (e.g., housekeeping biomarker), or the level or intrinsic gene score of a biomarker in a sample obtained prior to administration of a therapeutic agent (e.g., DMARD)) or compared to a reference level (e.g., a patient, e.g., a patient receiving a DMARD (e.g., methotrexate, hydroxychloroquine, The term "reduced expression" refers to a decreased expression of a biomarker, a decreased level of a biomarker, or a decreased eigengene score in an individual compared to a level in a sample previously obtained from an individual at a previous time point) compared to a median expression level or median eigengene score of a biomarker in samples from a group / population of patients with RA who are being tested for responsiveness to RA therapeutics, including DMARDs, sulfasalazine, leflunomide, azathioprine, cyclophosphamide, cyclosporine, or mycophenolate mofetil; a median expression level or median eigengene score of a biomarker in samples from a group / population of patients with RA who are identified as not responding to RA therapeutics, including DMARDs; or a level in a sample previously obtained from an individual at a previous time point. In some embodiments, reduced expression is little or no expression.

[0069] As used herein, a "reference sample", "reference cell", "reference tissue", "control sample", "control cell", or "control tissue" refers to a sample, cell, tissue, or standard used for comparison purposes. In one embodiment, the reference sample, reference cell, reference tissue, control sample, control cell, or control tissue is obtained from a healthy and / or non-diseased part (e.g., tissue or cell) of the body of the same patient or individual. For example, the reference sample, reference cell, reference tissue, control sample, control cell, or control tissue can be a healthy and / or non-diseased cell or tissue adjacent to a diseased cell or tissue (e.g., a cell or tissue adjacent to a diseased joint). In another embodiment, the reference sample is obtained from an untreated tissue and / or cell of the body of the same patient or individual. In yet another embodiment, the reference sample, reference cell, reference tissue, control sample, control cell, or control tissue is obtained from a healthy and / or non-diseased part (e.g., tissue or cell) of the body of an individual other than the patient or individual. In yet another embodiment, the reference sample, reference cell, reference tissue, control sample, control cell or control tissue is obtained from untreated tissues and / or cells from the body of an individual other than the patient or individual of interest. In another embodiment, the reference sample, reference cell, reference tissue, control sample, control cell, or control tissue is obtained from the patient prior to administration of a therapeutic agent (e.g., a RA therapeutic agent, including a DMARD (e.g., methotrexate, hydroxychloroquine, sulfasalazine, leflunomide, azathioprine, cyclophosphamide, cyclosporine, or mycophenolate mofetil) and / or a biologic therapeutic agent (e.g., a B-cell antagonist (e.g., rituximab), a Janus kinase (JAK) antagonist (e.g., tofacitinib), a tumor necrosis factor (TNF) antagonist, a decoy TNF receptor, a T-cell costimulatory signal antagonist, an IL-1 receptor antagonist, or an IL-6 receptor antagonist (tocilizumab)).

[0070] The term "housekeeping biomarker" refers to a biomarker or group of biomarkers (e.g., polynucleotides and / or polypeptides) that are typically present similarly in all cell types. In some embodiments, a housekeeping biomarker is a "housekeeping gene." "Housekeeping gene" refers herein to a gene or group of genes whose activity is essential for maintaining cellular function and that encodes a protein that is typically present similarly in all cell types. Exemplary housekeeping genes can include, but are not limited to, ACTB, GAPDH, GUSB, HPRT1, PGK1, RPL19, TUBB, and TMEM55B.

[0071] The term "ACTB" as used herein refers to any native beta-actin from any vertebrate source, including mammals such as primates (e.g., humans) and rodents (e.g., mice and rats), unless otherwise indicated. The term encompasses "full-length" unprocessed ACTB as well as any form of ACTB resulting from processing in cells. The term also encompasses naturally occurring variants of ACTB, such as splice variants of ACTB, such as splice variants or allelic variants. ACTB is also referred to in the art as beta (β)-actin, actin beta, PS1TP5 binding protein 1, beta cytoskeletal actin, PS1TP5BP1, BRWS1, and actin, cytoplasmic 1. An exemplary nucleic acid sequence of human ACTB is shown under NCBI Reference Sequence: NM_002046.6, or in SEQ ID NO: 15. The amino acid sequence of an exemplary protein encoded by human ACTB is shown under UniProt accession number P60709-1, or in SEQ ID NO:16.

[0072] The term "GAPDH" as used herein refers to any native glyceraldehyde-3-phosphate dehydrogenase from any vertebrate source, including mammals such as primates (e.g., humans) and rodents (e.g., mice and rats), unless otherwise indicated. The term encompasses "full-length" unprocessed GAPDH as well as any form of GAPDH resulting from processing in cells. The term also encompasses naturally occurring variants of GAPDH, such as splice variants of GAPDH, such as splice variants or allelic variants. GAPDH is also referred to in the art as GAPD, EC1.2.1.12, epididymal secretory sperm binding protein Li 162eP, senescence-associated gene 9 protein, HEL-S-162eP, HEL-S-162eP, EC1.2.1, G3PD, G3PDH, and peptidyl-cysteine ​​S-nitrosylase GAPDH. An exemplary nucleic acid sequence of human GAPDH is set forth under NCBI Reference Sequence: NM_002046.6, or in SEQ ID NO: 17. An exemplary amino acid sequence of a protein encoded by human GAPDH is set forth under UniProt Accession No. P04406, or in SEQ ID NO: 18.

[0073] The term "GUSB" as used herein refers to any naturally occurring glucuronidase beta from any vertebrate source, including mammals such as primates (e.g., humans) and rodents (e.g., mice and rats), unless otherwise indicated. The term encompasses "full-length" unprocessed GUSB as well as any form of GUSB resulting from processing in cells. The term also encompasses naturally occurring variants of GUSB, such as splice variants of GUSB, such as splice variants or allelic variants. GUSB is also referred to in the art as EC3.2.1.31, beta-G1, beta-D-glucuronidase, beta-glucuronidase, MPS7, BG, and glucuronidase, beta. An exemplary nucleic acid sequence of human GUSB is shown under NCBI Reference Sequence: NM_000181.3, or in SEQ ID NO: 19. The amino acid sequence of an exemplary protein encoded by human GUSB is shown under UniProt accession number P08236, or in SEQ ID NO:20.

[0074] The term "HPRT1" as used herein refers to any native hypoxanthine phosphoribosyltransferase 1 from any vertebrate source, including mammals such as primates (e.g., humans) and rodents (e.g., mice and rats), unless otherwise indicated. The term encompasses "full-length" unprocessed HPRT1 as well as any form of HPRT1 resulting from processing in cells. The term also encompasses naturally occurring variants of HPRT1, such as splice variants of HPRT1, such as splice variants or allelic variants. HPRT1 is also referred to in the art as EC 2.4.2.8, HGPRTase, HGPRT, HPRT, hypoxanthine-guanine phosphoribosyltransferase 1, hypoxanthine-guanine phosphoribosyltransferase, and testis tissue protein Li89. An exemplary nucleic acid sequence of human HPRT1 is set forth under NCBI Reference Sequence: NM_000194.2, or in SEQ ID NO: 21. An exemplary amino acid sequence of a protein encoded by human HPRT1 is set forth under UniProt Accession Number P00492, or in SEQ ID NO: 22.

[0075] The term "PGK1" as used herein refers to any native phosphoglycerate kinase 1 from any vertebrate source, including mammals such as primates (e.g., humans) and rodents (e.g., mice and rats), unless otherwise indicated. The term encompasses "full-length" unprocessed PGK1 as well as any form of PGK1 resulting from processing in cells. The term also encompasses naturally occurring variants of PGK1, such as splice variants of PGK1, such as splice variants or allelic variants. PGK1 is also referred to in the art as cell migration-inducing gene 10 protein, primer recognition protein 2, EC2.7.2.3, PRP2, PGKA, epididymal secretory sperm-binding protein Li 68p, HEL-S-68p, and MIG10. An exemplary nucleic acid sequence of human PGK1 is shown under NCBI Reference Sequence: NM_000291.3, or in SEQ ID NO:23. The amino acid sequence of an exemplary protein encoded by human PGK1 is shown under UniProt accession number P00558, or in SEQ ID NO:24.

[0076] The term "RPL19" as used herein refers to any native ribosomal protein L19 from any vertebrate source, including mammals such as primates (e.g., humans) and rodents (e.g., mice and rats), unless otherwise indicated. The term encompasses "full-length" unprocessed RPL19 as well as any form of RPL19 resulting from processing in cells. The term also encompasses naturally occurring variants of RPL19, such as splice variants of RPL19, such as splice variants or allelic variants. RPL19 is also referred to in the art as ribosomal large subunit protein EL19, ribosomal large subunit protein EL19, ribosomal protein L19, cytosolic, N-terminal truncated, and L19. An exemplary nucleic acid sequence of human RPL19 is shown under NCBI Reference Sequence: NM_000981.3, or in SEQ ID NO:25. The amino acid sequence of an exemplary protein encoded by human RPL19 is shown under UniProt accession number P84098, or in SEQ ID NO:26.

[0077] The term "TUBB" as used herein refers to any native tubulin beta class I from any vertebrate source, including mammals such as primates (e.g., humans) and rodents (e.g., mice and rats), unless otherwise indicated. The term encompasses "full-length" unprocessed TUBB as well as any form of TUBB resulting from processing in cells. The term also encompasses naturally occurring variants of TUBB, such as splice variants of TUBB, such as splice variants or allelic variants. TUBB is also referred to in the art as tubulin, beta polypeptide, tubulin beta-5 chain, TUBB5, class I beta-tubulin, tubulin beta-1 chain, tubulin beta chain, beta Ib tubulin, beta 1-tubulin, tubulin, beta, OK / SW-Cl.56, CDCBM6, CSCSC1, TUBB1, and M40. An exemplary nucleic acid sequence of human TUBB is shown under NCBI Reference Sequence: NM_001293212.1, or in SEQ ID NO: 27. An exemplary amino acid sequence of a protein encoded by human TUBB is shown under UniProt Accession Number P07437, or in SEQ ID NO: 28.

[0078] The term "TMEM55B" as used herein, unless otherwise indicated, refers to any native transmembrane protein 55B from any vertebrate source, including mammals such as primates (e.g., humans) and rodents (e.g., mice and rats). The term encompasses "full-length" unprocessed TMEM55B as well as any form of TMEM55B resulting from processing in a cell. The term also encompasses naturally occurring variants of TMEM55B, such as splice variants of TMEM55B, such as splice variants or allelic variants. TMEM55B is also referred to in the art as phosphatidylinositol-4,5-bisphosphate 4-phosphatase 1, PIP4P1, type I phosphatidylinositol-4,5-bisphosphate 4-phosphatase, type I PtdIns-4,5-P(2) 4-phosphatase, type 1 PtdIns-4,5-P2 4-Ptase, PtdIns-4,5-P2 4-Ptase I, EC 3.1.3.78, C14orf9, type 1 phosphatidylinositol 4,5-bisphosphate 4-phosphatase, PtdIns-4,5-P(2) 4-phosphatase type I, and chromosome 14 open reading frame 9. An exemplary nucleic acid sequence of human TMEM55B is set forth under NCBI Reference Sequence: NM_001100814.2 or in SEQ ID NO:29. The amino acid sequence of an exemplary protein encoded by human TMEM55B is shown under UniProt accession number Q86T03, or in SEQ ID NO:30.

[0079] "Correlating" or "correlating" means comparing in any manner the performance and / or results of a first analysis or protocol with the performance and / or results of a second analysis or protocol. For example, the results of the first analysis or protocol may be used in carrying out a second protocol and / or may be used to determine whether a second analysis or protocol should be performed. With respect to embodiments of a polypeptide analysis or protocol, the results of a polypeptide expression analysis or protocol may be used to determine whether a particular treatment regimen should be performed. With respect to embodiments of a polynucleotide analysis or protocol, the results of a polynucleotide expression analysis or protocol may be used to determine whether a particular treatment regimen should be performed.

[0080] "Amplification," as used herein, generally refers to the process of generating multiple copies of a desired sequence. "Multiple copies" means at least two copies. A "copy" does not necessarily mean perfect sequence complementarity or identity to the template sequence. For example, a copy can include nucleotide analogs such as deoxyinosine, deliberate sequence changes (such as those introduced through a primer that contains a sequence that is hybridizable to, but not complementary to, the template), and / or sequence errors that occur during amplification.

[0081] The technique of "polymerase chain reaction" or "PCR" as used herein generally refers to a procedure in which minute specific pieces of nucleic acid, RNA, and / or DNA are amplified, for example, as described in U.S. Pat. No. 4,683,195. Generally, sequence information from or beyond the ends of the region of interest must be available so that oligonucleotide primers can be designed. These primers will be identical or similar in sequence to opposite strands of the template to be amplified. The 5' terminal nucleotides of the two primers may match the ends of the amplified material. PCR can be used to amplify specific RNA sequences, specific DNA sequences from total genomic DNA, cDNA transcribed from total cellular RNA, bacteriophage, or plasmid sequences, etc. See generally Mullis et al., Cold Spring Harbor Symp. Quant. Biol. 51:263 (1987) and Erlich, ed., PCR Technology, (Stockton Press, NY, 1989). As used herein, PCR is considered to be one example, but not the only example, of a nucleic acid polymerase reaction method for amplifying a nucleic acid test sample, including the use of a known nucleic acid (DNA or RNA) as a primer, and utilizes a nucleic acid polymerase to amplify or generate a specific piece of nucleic acid, or a specific piece of nucleic acid complementary to a specific nucleic acid.

[0082] The term "multiplex PCR" refers to a single PCR reaction performed on nucleic acid obtained from a single source (e.g., an individual) using more than one primer set to amplify two or more DNA sequences in a single reaction.

[0083] "Quantitative real-time polymerase chain reaction" or "qRT-PCR" refers to a form of PCR in which the amount of PCR product is measured at each step in the PCR reaction. This technique is described in various publications, including, for example, Cronin et al., Am. J. Pathol. 164(1):35-42 (2004) and Ma et al., Cancer Cell 5:607-616 (2004).

[0084] The term "microarray" refers to an ordered arrangement of hybridizable array elements, preferably polynucleotide probes, on a substrate.

[0085] The term "RNAseq", also known as "RNA-seq" or "whole transcriptome shotgun sequencing (WTSS)", refers to the use of high-throughput sequencing techniques to sequence and / or quantify cDNA to obtain information about the RNA content of a sample. Publications describing RNAseq include Wang et al. Nature Reviews Genetics 10(1):57-63, 2009, Ryan et al. BioTechniques 45(1):81-94, 2008, and Maher et al. Nature 458(7234):97-101, 2009.

[0086] "Polynucleotide" or "nucleic acid", as used interchangeably herein, refers to a polymer of nucleotides of any length, including DNA and RNA. The nucleotides can be deoxyribonucleotides, ribonucleotides, modified nucleotides or bases, and / or their analogs, or any substrate that can be incorporated into a polymer by DNA or RNA polymerase or by a synthetic reaction. Thus, for example, polynucleotides as defined herein include, but are not limited to, single-stranded and double-stranded DNA, DNA containing single-stranded and double-stranded regions, single-stranded and double-stranded RNA, and RNA containing single-stranded and double-stranded regions, hybrid molecules containing DNA and RNA that may be single-stranded or, more typically, double-stranded, or may contain single-stranded and double-stranded regions. In addition, the term "polynucleotide" as used herein refers to triple-stranded regions that contain RNA or DNA, or both RNA and DNA. The strands in such regions may be from the same molecule or from different molecules. These regions may include all of one or more of the molecules, but more typically involve only certain regions of some of the molecules. One of the molecules in the triple helix region is often an oligonucleotide. The terms "polynucleotide" and "nucleic acid" specifically include mRNA and cDNA.

[0087] A polynucleotide may comprise modified nucleotides, such as methylated nucleotides and their analogs. If present, modifications to the nucleotide structure may be imparted before or after assembly of the polymer. The sequence of nucleotides may be interrupted by non-nucleotide components. A polynucleotide may be further modified after synthesis, such as by conjugation with a label. Other types of modifications include, for example, "caps", replacement of one or more of the naturally occurring nucleotides with an analog, internucleotide modifications such as, for example, those with uncharged linkages (e.g., methylphosphonates, phosphotriesters, phosphoamidates, carbamates, etc.) and those with charged linkages (e.g., phosphorothioates, phosphorodithioates, etc.), those containing pendant moieties such as proteins (e.g., nucleases, toxins, antibodies, signal peptides, poly-L-lysine, etc.), those containing intercalating agents (e.g., acridine, psoralen, etc.), those containing chelators (e.g., metals, radioactive metals, boron, oxidative metals, etc.), those containing alkylating agents, those with modified linkages (e.g., alpha anomeric nucleic acids), as well as unmodified forms of the polynucleotide(s). Additionally, any of the hydroxyl groups normally present on the sugar may be replaced, for example, by phosphonate groups, phosphate groups, protected by standard protecting groups, or activated to prepare additional bonds to additional nucleotides, or conjugated to solid or semi-solid supports. The 5' and 3' terminal OH may be phosphorylated or substituted with amines or organic capping group moieties of 1-20 carbon atoms. Other hydroxyls may also be derivatized to standard protecting groups. Polynucleotides may also contain analogous forms of ribose or deoxyribose sugars well known in the art, including, for example, 2'-O-methyl-, 2'-O-allyl-, 2'-fluoro-, or 2'-azido-ribose, carbocyclic sugar analogs, α-anomeric sugars, epimeric sugars such as arabinose, xylose, or lyxose, pyranose sugars, furanose sugars, sedoheptulose, acyclic analogs, and abasic nucleoside analogs such as methyl riboside.One or more phosphodiester linkages may be replaced by alternative linking groups. These alternative linking groups include, but are not limited to, embodiments in which the phosphate is replaced by P(O)S ("thioate"), P(S)S ("dithioate"), "(O)NR2 ("amidate"), P(O)R, P(O)OR', CO, or CH2 ("formacetal"), where each R or R' is independently H or a substituted or unsubstituted alkyl (1-20C), aryl, alkenyl, cycloalkyl, cycloalkenyl, or araldyl, optionally containing an ether (-O-) linkage. Not all linkages in a polynucleotide need be identical. The foregoing description applies to all polynucleotides referred to herein, including RNA and DNA.

[0088] As used herein, "oligonucleotide" generally refers to a short single-stranded polynucleotide that is, but is not necessarily, less than about 250 nucleotides in length. Oligonucleotides may be synthetic. The terms "oligonucleotide" and "polynucleotide" are not mutually exclusive. The above description of polynucleotides is equally and fully applicable to oligonucleotides.

[0089] The terms "primer" or "probe," as used interchangeably herein, generally refer to a single-stranded polynucleotide that can hybridize to a nucleic acid, by providing a free 3'-OH group, to effect polymerization of a complementary nucleic acid.

[0090] An "isolated" nucleic acid molecule is a nucleic acid molecule that is identified and separated from at least one contaminant nucleic acid molecule with which it is normally associated in the natural source of the nucleic acid. An isolated nucleic acid molecule is other than in the form or setting in which it is found in nature. An isolated nucleic acid molecule is therefore distinct from the nucleic acid molecule as it exists in natural cells.

[0091] The term "diagnosis" is used herein to refer to the identification or classification of a molecular or pathological state, disease or condition (e.g., RA).For example, "diagnosis" can refer to the classification of a particular pathotype of RA, for example, by histopathological criteria or by molecular features (e.g., subtypes characterized by the expression of one or a combination of biomarkers (e.g., specific genes or proteins encoded by said genes).

[0092] As used herein, the terms "individual", "patient" and "subject" are used interchangeably and refer to any single animal, more preferably a mammal (including, for example, cats, dogs, horses, rabbits, zoo animals, cows, pigs, sheep, and non-human animals such as non-human primates), for which treatment is desired. In certain embodiments, the patient herein is a human. The patient may be an "RA patient", i.e., one who suffers from RA, or one who is at risk of suffering from RA, or one who suffers from one or more symptoms of RA.

[0093] As used herein, "treatment" (and grammatical variations thereof, such as "treat" or "treating") refers to a clinical intervention that seeks to alter the natural course of the individual being treated, and can be performed either prophylactically or during the clinical pathology course. Desirable effects of treatment include, but are not limited to, prevention of disease onset or recurrence, alleviation of symptoms, diminishment of any direct or indirect pathological consequences of the disease, reduction in the rate of disease progression, amelioration or temporary relief of the disease state, and remission or improvement of prognosis. In some embodiments, the treatment is with RA therapies including DMARDs (e.g., methotrexate, hydroxychloroquine, sulfasalazine, leflunomide, azathioprine, cyclophosphamide, cyclosporine, or mycophenolate mofetil) and / or biologic therapeutics (e.g., B-cell antagonists (e.g., rituximab), Janus kinase (JAK) antagonists (e.g., tofacitinib), tumor necrosis factor (TNF) antagonists, decoy TNF receptors, T-cell costimulatory signal antagonists, IL-1 receptor antagonists, or IL-6 receptor antagonists (e.g., tocilizumab)) used to delay disease onset or slow the progression of a disease or disorder (e.g., RA).

[0094] As used herein, "administering" refers to administering a dosage of a compound (e.g., a DMARD (e.g., methotrexate, hydroxychloroquine, sulfasalazine, leflunomide, azathioprine, cyclophosphamide, cyclosporine, or mycophenolate mofetil) and / or a biologic therapeutic agent (e.g., a B-cell antagonist (e.g., rituximab), a Janus kinase (JAK) antagonist (e.g., tofacitinib), a tumor necrosis factor (TN) antagonist (e.g., a vasodilator ... F) antagonists, decoy TNF receptors, T cell costimulatory signal antagonists, IL-1 receptor antagonists, or IL-6 receptor antagonists (e.g., tocilizumab) to an individual. Compositions utilized in the methods described herein can be administered, for example, orally, subcutaneously, intramuscularly, intravenously, intradermally, percutaneously, intraarterially, intraperitoneally, intralesionally, intracranially, intraarticularly, intraprostatically, intrapleurally, intratracheally, intrathecally, intrathecally, intraperitoneally ... The compositions may be administered intracavitarily, intranasally, intravaginally, intrarectally, topically, intratumorally, peritoneally, subconjunctivally, intravesicularly, mucosally, intrapericardially, intraumbilically, intraocularly, intraorbitally, intravitreally (e.g., by intravitreal injection), by eye drop, topically, transdermally, parenterally, by inhalation, by injection, by implantation, by infusion, by continuous infusion, by localized irrigation that directly bathes the target cells, by catheter, by lavage, in a cream, or in a lipid composition. In some cases, the compositions utilized in the methods described herein may be administered orally. In some cases, the compositions utilized in the methods described herein may be administered subcutaneously. The compositions utilized in the methods described herein may also be administered systemically or locally. The method of administration may vary depending on various factors, such as the compound or composition to be administered and the severity of the condition, disease, or disorder (e.g., RA) to be treated.

[0095] The term "concurrently" is used herein to refer to administration of two or more therapeutic agents, where at least a portion of the administration overlaps in time. Thus, concurrent administration includes dosing regimens where administration of one or more agent(s) continues after administration of one or more other agent(s) is discontinued.

[0096] "Reducing or inhibiting" refers to the ability to cause an overall decrease of 20%, 30%, 40%, 50%, 60%, 70%, 75%, 80%, 85%, 90%, 95% or more. Reducing or inhibiting can refer, for example, to the symptoms of the disorder (e.g., RA) being treated.

[0097] "Therapeutically effective amount" or "effective amount" refers to the amount of a therapeutic agent that treats or prevents a disease or disorder (e.g., RA) in a mammal. In the example of RA, a therapeutically effective amount of a therapeutic agent can reduce the likelihood of rheumatoid arthritis, reduce the occurrence of rheumatoid arthritis, and / or reduce the severity of rheumatoid arthritis, preferably to the extent that the individual no longer experiences discomfort and / or altered function due to it. For example, a therapeutically effective amount can refer to the amount of a therapeutic agent required to prevent the occurrence of rheumatoid arthritis and / or cure or alleviate the symptoms, signs, or causes of rheumatoid arthritis when administered to a subject. A therapeutically effective amount also refers to the amount of a therapeutic agent required to relieve or reduce at least one disease activity indicator and / or clinical symptom, and / or inhibit, delay, or reverse the progression of a pathology, and / or prevent or delay the onset of a disease or condition. In the case of RA therapeutics, in vivo efficacy can be determined, for example, by using ACR and / or European League Against Rheumatism (EULAR) clinical response parameters in patients with RA, or by assaying molecular determinants of the extent of RA in patients.

[0098] The term "antagonist" is used in its broadest sense and includes any molecule that partially or completely blocks, inhibits, or neutralizes the biological activity of a native polypeptide. In a similar manner, the term "agonist" is used in its broadest sense and includes any molecule that mimics the biological activity of a native polypeptide. Suitable agonist or antagonist molecules specifically include agonist or antagonist antibodies or antibody fragments, fragments or amino acid sequence variants of a native polypeptide, small molecule inhibitors, and the like. A method for identifying an agonist or antagonist of a polypeptide may include contacting the polypeptide with a candidate agonist or antagonist molecule and measuring a detectable change in one or more biological activities normally associated with the polypeptide.

[0099] As used herein, a "B cell antagonist" is any molecule that reduces, blocks, inhibits, abrogates, or interferes with signal transduction resulting from the interaction of a B cell with one or more of its interaction partners. In some embodiments, a B cell antagonist is a molecule that, upon binding to a B cell surface marker, destroys or depletes B cells in a mammal and / or interferes with one or more B cell functions, for example, by reducing or preventing a humoral response elicited by the B cell. The antagonist in certain cases can also deplete B cells (i.e., reduce circulating B cell levels) in a mammal treated therewith. Such depletion may be achieved through various mechanisms, such as ADCC and / or CDC, inhibition of B cell proliferation, and / or induction of B cell death (e.g., via apoptosis). In some embodiments, a "B cell antagonist" is a molecule that inhibits the binding of CD20 to its binding partner. In some embodiments, a B cell antagonist inhibits activation of CD20. In some embodiments, the B cell antagonist includes anti-CD20 antibodies (e.g., rituximab), antigen-binding fragments thereof, immunoadhesins, fusion proteins, oligopeptides, and other molecules that reduce, block, inhibit, abrogate, or prevent signal transduction resulting from the interaction of CD20 with one or more of its interaction partners. In some embodiments, the B cell antagonist is a polypeptide, a small molecule, or a nucleic acid. In some embodiments, the B cell antagonist (e.g., a B cell binding antagonist) inhibits CD20. In certain embodiments, the B cell antagonist has a binding affinity (dissociation constant) of about 1,000 nM or less for B cells or molecules expressed on their surface. In another embodiment, the B cell antagonist has a binding affinity of about 100 nM or less for B cells or molecules expressed on their surface. In another embodiment, the B cell antagonist has a binding affinity of about 50 nM or less for B cells or molecules expressed on their surface.In another embodiment, the B cell antagonist has a binding affinity for B cells or molecules expressed on their surface of about 10 nM or less. In another embodiment, the B cell antagonist has a binding affinity for B cells or molecules expressed on their surface of about 1 nM or less. In a particular embodiment, the B cell antagonist inhibits B cell signaling with an IC50 of 1,000 nM or less. In another embodiment, the B cell antagonist inhibits B cell signaling with an IC50 of 500 nM or less. In another embodiment, the B cell antagonist inhibits B cell signaling with an IC50 of 50 nM or less. In another embodiment, the B cell antagonist inhibits B cell signaling with an IC50 of 10 nM or less. In another embodiment, the B cell antagonist inhibits B cell signaling with an IC50 of 1 nM or less.

[0100] A "Janus kinase antagonist" or "JAK antagonist," as used interchangeably herein, is any molecule that reduces, blocks, inhibits, abrogates, or interferes with signal transduction resulting from the interaction of a Janus kinase with one or more of its interaction partners. In some embodiments, a Janus kinase antagonist is a molecule that, upon binding to a Janus kinase, reduces, blocks, inhibits, abrogates, or interferes with the activation or function of JAK / signal transducer and activator of transcription (STAT) in a mammal treated therewith. In some embodiments, Janus kinase antagonists include antibodies (e.g., tofacitinib), antigen-binding fragments thereof, immunoadhesins, fusion proteins, oligopeptides, and other molecules that reduce, block, inhibit, abrogate, or interfere with signal transduction resulting from the interaction of a Janus kinase with one or more of its interaction partners. In some embodiments, a Janus kinase antagonist is a polypeptide, a small molecule, or a nucleic acid. In some embodiments, the Janus kinase antagonist inhibits Janus kinase. In certain embodiments, the Janus kinase antagonist has a binding affinity (dissociation constant) of about 1,000 nM or less to Janus kinase. In another embodiment, the Janus kinase antagonist has a binding affinity of about 100 nM or less to Janus kinase. In another embodiment, the Janus kinase antagonist has a binding affinity of about 50 nM or less to Janus kinase. In another embodiment, the Janus kinase antagonist has a binding affinity of about 10 nM or less to Janus kinase. In another embodiment, the Janus kinase antagonist has a binding affinity of about 1 nM or less to Janus kinase. In certain embodiments, the Janus kinase antagonist inhibits Janus kinase signaling with an IC50 of 1,000 nM or less. In another embodiment, the Janus kinase antagonist inhibits Janus kinase signaling with an IC50 of 500 nM or less. In another embodiment, the Janus kinase antagonist inhibits Janus kinase signaling with an IC50 of 50 nM or less.In another embodiment, the Janus kinase antagonist inhibits Janus kinase signaling with an IC50 of 10 nM or less. In another embodiment, the Janus kinase antagonist inhibits Janus kinase signaling with an IC50 of 1 nM or less.

[0101] A "tumor necrosis factor antagonist" or "TNF antagonist", as used interchangeably herein, is any molecule that reduces, blocks, inhibits, abrogates, or interferes with signal transduction resulting from the interaction of TNF with one or more of its interaction partners. In some embodiments, a TNF antagonist is a molecule that, upon binding to TNF, reduces, blocks, inhibits, abrogates, or interferes with the activation or function of TNF in a mammal treated therewith. In some embodiments, TNF antagonists include antibodies, antigen-binding fragments thereof, immunoadhesins, fusion proteins, oligopeptides, and other molecules that reduce, block, inhibit, abrogate, or interfere with signal transduction resulting from the interaction of TNF with one or more of its interaction partners. In some embodiments, the TNF antagonist is a polypeptide, a small molecule, or a nucleic acid. In some embodiments, the TNF antagonist inhibits TNF. In certain embodiments, the TNF antagonist has a binding affinity (dissociation constant) for TNF of about 1,000 nM or less. In another embodiment, the TNF antagonist has a binding affinity for TNF of about 100 nM or less. In another embodiment, the TNF antagonist has a binding affinity for TNF of about 50 nM or less. In another embodiment, TNF has a binding affinity for TNF of about 10 nM or less. In another embodiment, the TNF antagonist has a binding affinity for TNF of about 1 nM or less. In a particular embodiment, the TNF antagonist inhibits TNF signaling with an IC50 of 1,000 nM or less. In another embodiment, the TNF antagonist inhibits TNF signaling with an IC50 of 500 nM or less. In another embodiment, the TNF antagonist inhibits TNF signaling with an IC50 of 50 nM or less. In another embodiment, the TNF antagonist inhibits TNF signaling with an IC50 of 10 nM or less. In another embodiment, the TNF antagonist inhibits TNF signaling with an IC50 of 1 nM or less.

[0102] A "decoy tumor necrosis factor receptor" or "decoy TNF receptor", as used interchangeably herein, is any molecule that reduces, blocks, inhibits, abrogates, or interferes with signal transduction resulting from the interaction of TNF with one or more of its interaction partners. In some embodiments, a decoy TNF receptor is a molecule that, upon binding to TNF, reduces, blocks, inhibits, abrogates, or interferes with the activation or function of TNF in a mammal treated therewith. In some embodiments, a decoy TNF receptor includes antibodies, antigen-binding fragments thereof, immunoadhesins, fusion proteins, oligopeptides, and other molecules that reduce, block, inhibit, abrogate, or interfere with signal transduction resulting from the interaction of TNF with one or more of its interaction partners. In some embodiments, a decoy TNF receptor is a polypeptide, a small molecule, or a nucleic acid. In some embodiments, a decoy TNF receptor inhibits TNF. In a particular embodiment, the decoy TNF receptor has a binding affinity (dissociation constant) for TNF of about 1,000 nM or less. In another embodiment, the decoy TNF receptor has a binding affinity for TNF of about 100 nM or less. In another embodiment, the decoy TNF receptor has a binding affinity for TNF of about 50 nM or less. In another embodiment, the decoy TNF receptor has a binding affinity for TNF of about 10 nM or less. In another embodiment, the decoy TNF receptor has a binding affinity for TNF of about 1 nM or less. In another embodiment, the decoy TNF receptor has a binding affinity for TNF of about 1 nM or less. In a particular embodiment, the decoy TNF receptor inhibits TNF signaling with an IC50 of 1,000 nM or less. In another embodiment, the decoy TNF receptor inhibits TNF signaling with an IC50 of 500 nM or less. In another embodiment, the decoy TNF receptor inhibits TNF signaling with an IC50 of 50 nM or less. In another embodiment, the decoy TNF receptor inhibits TNF signaling with an IC50 of 10 nM or less.In another embodiment, the decoy TNF receptor inhibits TNF signaling with an IC50 of 1 nM or less.

[0103] As used herein, a "T cell costimulatory signal antagonist" is any molecule that reduces, blocks, inhibits, abrogates, or interferes with signal transduction resulting from the interaction of a T cell with one or more of its interaction partners. In some embodiments, a T cell costimulatory signal antagonist is a molecule that, upon binding to a T cell, reduces, blocks, inhibits, abrogates, or interferes with T cell activation. In some embodiments, a "T cell costimulatory signal antagonist" is a molecule that inhibits the binding of CD80 and CD86 to their binding partners. In some embodiments, a T cell costimulatory signal antagonist inhibits activation of a T cell. In some embodiments, a T cell costimulatory signal antagonist includes antibodies, antigen-binding fragments thereof, immunoadhesins, fusion proteins, oligopeptides, and other molecules that reduce, block, inhibit, abrogate, or interfere with signal transduction resulting from the interaction of a T cell with one or more of its interaction partners. In some embodiments, a T cell costimulatory signal antagonist is a polypeptide, a small molecule, or a nucleic acid. In some embodiments, the T cell costimulatory signal antagonist inhibits T cells. In certain embodiments, the T cell costimulatory signal antagonist has a binding affinity (dissociation constant) of about 1,000 nM or less to T cells or molecules expressed on their surface. In another embodiment, the T cell costimulatory signal antagonist has a binding affinity of about 100 nM or less to T cells or molecules expressed on their surface. In another embodiment, the T cell costimulatory signal antagonist has a binding affinity of about 50 nM or less to T cells. In another embodiment, the T cell costimulatory signal antagonist has a binding affinity of about 10 nM or less to T cells or molecules expressed on their surface. In another embodiment, the T cell costimulatory signal antagonist has a binding affinity of about 1 nM or less to T cells or molecules expressed on their surface. In certain embodiments, the T cell costimulatory signal antagonist inhibits T cell signaling with an IC50 of 1,000 nM or less.In another embodiment, the T cell costimulatory signal antagonist inhibits T cell signaling with an IC50 of 500 nM or less. In another embodiment, the T cell costimulatory signal antagonist inhibits T cell signaling with an IC50 of 50 nM or less. In another embodiment, the T cell costimulatory signal antagonist inhibits T cell signaling with an IC50 of 10 nM or less. In another embodiment, the T cell costimulatory signal antagonist inhibits T cell signaling with an IC50 of 1 nM or less.

[0104] An "IL-1 receptor antagonist", "interleukin 1 receptor antagonist", or "IL-1R antagonist", as used interchangeably herein, is any molecule that reduces, blocks, inhibits, abrogates, or interferes with signal transduction resulting from the interaction of the IL-1 receptor with one or more of its interaction partners. In some embodiments, an IL-1 receptor antagonist is a molecule that, upon binding to the IL-1 receptor, reduces, blocks, inhibits, abrogates, or interferes with the activation or function of the IL-1R in a mammal treated therewith. In some embodiments, IL-1 receptor antagonists include antibodies, antigen-binding fragments thereof, immunoadhesins, fusion proteins, oligopeptides, and other molecules that reduce, block, inhibit, abrogate, or interfere with signal transduction resulting from the interaction of the IL-1 receptor with one or more of its interaction partners. In some embodiments, the IL-1 receptor antagonist is a polypeptide, a small molecule, or a nucleic acid. In some embodiments, the IL-1 receptor antagonist (e.g., IL-1 receptor binding antagonist) inhibits IL-1. In certain embodiments, the IL-1R inhibitor has a binding affinity (dissociation constant) for IL-1R of about 1,000 nM or less. In another embodiment, the IL-1R antagonist has a binding affinity for IL-1R of about 100 nM or less. In another embodiment, the IL-1R antagonist has a binding affinity for IL-1R of about 50 nM or less. In another embodiment, the IL-1R antagonist has a binding affinity for IL-1R of about 10 nM or less. In another embodiment, the IL-1R antagonist has a binding affinity for IL-1R of about 1 nM or less. In another embodiment, the IL-1R antagonist has a binding affinity for IL-1R of about 1 nM or less. In certain embodiments, the IL-1R antagonist inhibits IL-1R signaling with an IC50 of 1,000 nM or less. In another embodiment, the IL-1R antagonist inhibits IL-1R signaling with an IC50 of 500 nM or less. In another embodiment, the IL-1R antagonist inhibits IL-1R signaling with an IC50 of 50 nM or less.In another embodiment, the IL-1R antagonist inhibits IL-1R signaling with an IC50 of 10 nM or less. In another embodiment, the IL-1R antagonist inhibits IL-1R signaling with an IC50 of 1 nM or less.

[0105] An "IL-6 receptor antagonist", "interleukin 6 receptor antagonist", or "IL-6R antagonist", as used interchangeably herein, is any molecule that reduces, blocks, inhibits, abrogates, or interferes with signal transduction resulting from the interaction of the IL-6 receptor with one or more of its interaction partners. In some embodiments, an IL-6 receptor antagonist is a molecule that, upon binding to the IL-6 receptor, reduces, blocks, inhibits, abrogates, or interferes with the activation or function of IL-6R in a mammal treated therewith. In some embodiments, IL-6 receptor antagonists include antibodies (e.g., tocilizumab), antigen-binding fragments thereof, immunoadhesins, fusion proteins, oligopeptides, and other molecules that reduce, block, inhibit, abrogate, or interfere with signal transduction resulting from the interaction of the IL-6 receptor with one or more of its interaction partners. In some embodiments, the IL-6 receptor antagonist is a polypeptide, a small molecule, or a nucleic acid. In some embodiments, the IL-6 receptor antagonist (e.g., IL-6 receptor binding antagonist) inhibits IL-6. In a particular embodiment, the IL-6R antagonist has a binding affinity (dissociation constant) of about 1,000 nM or less to IL-6R. In another embodiment, the IL-6R antagonist has a binding affinity of about 100 nM or less to IL-6R. In another embodiment, the IL-6R antagonist has a binding affinity of about 50 nM or less to IL-6R. In another embodiment, the IL-6R antagonist has a binding affinity of about 10 nM or less to IL-6R. In another embodiment, the IL-6R antagonist has a binding affinity of about 1 nM or less to IL-6R. In a particular embodiment, the IL-6R antagonist inhibits IL-6R signal transduction with an IC50 of 1,000 nM or less. In another embodiment, the IL-6R antagonist inhibits IL-6R signaling with an IC50 of 500 nM or less. In another embodiment, the IL-6R antagonist inhibits IL-6R signaling with an IC50 of 50 nM or less.In another embodiment, the IL-6R antagonist inhibits IL-6R signaling with an IC50 of 10 nM or less. In another embodiment, the IL-6R antagonist inhibits IL-6R signaling with an IC50 of 1 nM or less.

[0106] The term "antibody" herein is used in the broadest sense and specifically includes monoclonal antibodies, polyclonal antibodies, half antibodies, multispecific antibodies (e.g., bispecific antibodies) formed from at least two intact antibodies, and antibody fragments, so long as they exhibit the desired biological activity. The term "immunoglobulin" (Ig) is used interchangeably with antibody herein.

[0107] A "blocking" antibody or antibody "antagonist" is one that inhibits or reduces the biological activity of the antigen to which it binds. For example, an IL-6 receptor antagonist antibody binds to the IL-6 receptor and reduces, blocks, inhibits, suppresses, or prevents signal transduction resulting from the interaction of the IL-6 receptor with IL-6. A preferred blocking or antagonist antibody completely inhibits the biological activity of the antigen.

[0108] An "article of manufacture" is any article of manufacture (e.g., package or container) or kit that contains at least one reagent, e.g., a pharmaceutical for the treatment of a disease or disorder (e.g., RA), or a probe for specifically detecting a biomarker (e.g., one or more of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A), as described herein. In certain embodiments, the article of manufacture or kit is promoted, distributed, or sold as a unit for performing a method described herein.

[0109] The term "package insert" is used to refer to instructions customarily included in commercial packaging of a therapeutic product that contain information about the indications, usage, dosage, administration, concomitant therapy, contraindications, and / or warnings regarding use of that therapeutic product.

[0110] A "sterile" preparation is aseptic or free of all living microorganisms and their spores.

[0111] As used herein, the phrase "based on" means that information about one or more biomarkers is used to inform treatment decisions, information provided on a package insert, or marketing / promotional guidelines.

[0112] "Pharmaceutically acceptable carrier" refers to an ingredient in a pharmaceutical formulation, other than an active ingredient, that is non-toxic to a subject. Pharmaceutically acceptable carriers include, but are not limited to, buffers, excipients, stabilizers, or preservatives.

[0113] "Disease progression" or "progression," as used interchangeably herein, refers to radiographic progression and / or increased disease activity. Radiographic progression may be defined, for example, by an increase in ShSS (e.g., an increase in ShSS over a defined time frame). For example, the time frame may be a minimum of one year from the initial examination.

[0114] As used herein, "disease activity" or "rheumatoid arthritis disease activity" refers to the severity or intensity of rheumatoid arthritis, and may be determined, for example, by ACR and / or EULAR clinical indices, including, but not limited to, DAS28-ESR, DAS28-CRP, ESR level, CRP level, ACPA titer, RF titer, swollen joint count, VAS, assessment of joint damage (e.g., by x-ray, ultrasound synovial thickening (USST) assessment, or ultrasound power Doppler score (USPD)), or combinations thereof. A DAS28-ESR of <3.2 indicates low disease activity, while a DAS28-ESR of 3.2-5.1 indicates moderate disease activity, and a DAS28-ESR of >5.1 indicates the most severe disease activity. RF titer seropositivity indicates high disease activity, while RF seronegativity indicates low disease activity. An ESR level of greater than 28 mm / hour, a CRP level of greater than 1 mg / dL, a positive ACPA titer, a count of at least one swollen joint, and / or the presence of radiographic erosions or joint space narrowing are abnormal and indicate disease activity.

[0115] "Responsiveness" or "effective response" may be assessed using any endpoint that indicates benefit to an individual, including, but not limited to, (i) inhibition to some extent of disease progression, including slowing and complete prevention; (ii) reduction in the number of disease episodes and / or symptoms; (iii) reduction in lesion size; (iv) inhibition (i.e., reduction, slowing, or complete halt) of disease cell invasion into adjacent peripheral organs and / or tissues; (v) inhibition (i.e., reduction, slowing, or complete halt) of disease spread; (vi) reduction in autoimmune response (which may, but need not, result in regression or disappearance of disease lesions); (vii) alleviation to some extent of one or more symptoms associated with the disorder; (viii) increase in disease-free presentation period following treatment; and / or (ix) reduction in mortality at a given time point following treatment.

[0116] II. Method Provided herein are methods and assays for diagnosing patients with rheumatoid arthritis (RA); identifying individuals with RA who are likely to develop disease progression; identifying individuals with RA who may benefit from treatments that include therapeutic agents other than or in addition to disease-modifying antirheumatic drugs (DMARDs); identifying individuals with RA who may benefit from treatments that include DMARDs; determining whether individuals with RA are likely to respond to treatments with RA therapeutic agents, including DMARDs; selecting a therapy for an individual with RA; treating individuals with RA based on the diagnostic method of the present invention; optimizing the therapeutic efficacy of RA therapeutic agents; and monitoring the therapeutic efficacy of RA therapeutic agents.The methods and assays described herein are based on the finding that the expression level of at least one or more biomarkers described herein in samples (e.g., synovial tissue samples, synovial fluid samples, or combinations thereof) from individuals with RA can be used to predict the therapeutic efficacy of RA therapeutic agents, such as therapeutic agents that include DMARDs (i.e., DMARDs) or biological therapeutic agents (i.e., biological therapeutic agents) alone or in combination with DMARDs. Any of the methods and assays may further comprise determining a myeloid, lymphoid, and / or minor immune-fibrotic intrinsic gene score. Any of the methods and assays provided herein may further comprise administering a DMARD (e.g., a DMARD described in Section II-B below) to the individual. Accordingly, methods and assays are also provided herein for evaluating the expression of one or more biomarkers in a sample from an individual. Any of the methods provided herein may comprise administering to the individual a RA therapeutic agent other than or in addition to a DMARD (e.g., a RA therapeutic agent other than or in addition to a DMARD described in Section II-B below). Any of the methods may further comprise administering to the individual an effective amount of an additional therapeutic agent as described herein.

[0117] A. Diagnostic Methods and Assays (i) Prognostic Methods and Assays The present invention provides methods that may be used to identify individuals with RA who are prone to disease progression, the methods and assays comprising detecting the presence or absence of one or more genes set forth in Table 1 (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114 3, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, or 46 genes), wherein a change in the expression level of one or more genes compared to a reference expression level identifies the individual as likely to exhibit disease progression. TIFF0007652566000001.tif254170TIFF0007652566000002.tif90170

[0118] In some cases, the methods and assays provided herein can include detecting a gene or gene fragment of Table 2 (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 109, 109, 108, 109, 110, 111, 112, 113, 114, The method may involve determining an expression level of one or more genes listed in Table 2 or Table 3 (e.g., 1, 2, 3, 4, 5, or 6 genes listed in Table 3) or Table 3 (e.g., 1, 36, 37, 38, 39, or 40 genes listed in Table 3), where a change in the expression level of one or more genes listed in Table 2 or Table 3 compared to a reference expression level identifies the individual as likely to exhibit disease progression. TIFF0007652566000003.tif248170TIFF0007652566000004.tif65170

[0119] In some cases, the methods and assays provided herein may involve determining the expression level of one or more genes set forth in Table 2 (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, or 40 genes set forth in Table 2). For example, the method may include determining an expression level of one or more genes listed in Table 2, where an increase in the expression level of one or more genes listed in Table 2 compared to a reference expression level (e.g., an increase in expression level of about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or more) identifies the individual as likely to exhibit disease progression. In certain cases, there is an increased expression of one or more genes listed in Table 2 (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, or 40 genes listed in Table 2). The expression level is an increase of at least about 1.1x, 1.2x, 1.3x, 1.4x, 1.5x, 1.6x, 1.7x, 1.8x, 1.9x, 2x, 2.1x, 2.2x, 2.3x, 2.4x, 2.5x, 2.6x, 2.7x, 2.8x, 2.9x, 3x, 3.5x, 4x, 4.5x, 5x, 6x, 7x, 8x, 9x, 10x, 15x, 20x, 30x, 40x, 50x, 100x, 500x, or 1000x as compared to a reference expression level of the one or more genes.In some cases, an increased expression level of one or more genes listed in Table 2 (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, or 40 genes listed in Table 2) may be a reference for the one or more genes. The increase is at least about 1.1 fold, about 1.2 fold, about 1.3 fold, about 1.4 fold, about 1.5 fold, about 1.6 fold, about 1.7 fold, about 1.8 fold, about 1.9 fold, about 2 fold, about 2.1 fold, about 2.2 fold, about 2.3 fold, about 2.4 fold, about 2.5 fold, about 2.6 fold, about 2.7 fold, about 2.8 fold, about 2.9 fold, about 3 fold, about 3.5 fold, about 4 fold, about 4.5 fold, about 5 fold, about 6 fold, about 7 fold, about 8 fold, about 9 fold, about 10 fold, about 15 fold, about 20 fold, about 30 fold, about 40 fold, about 50 fold, about 100 fold, about 500 fold, or about 1,000 fold or more as compared to the control expression level.

[0120] In certain cases, the methods and assays may include determining the expression level of one or more genes selected from CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A (e.g., 1, 2, 3, 4, 5, 6, or 7 genes selected from CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A) in a sample from an individual. For example, the method or assay may include determining the expression level of one or more genes selected from CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, where an increase in the expression level of one or more genes selected from CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A compared to a reference expression level (e.g., an increase of about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or more in expression level) identifies the individual as likely to exhibit disease progression. In certain cases, an increased expression level of one or more genes selected from CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A (e.g., 1, 2, 3, 4, 5, 6, or 7 genes selected from CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A) may be indicative of a reference expression level of the one or more genes. An increase of at least about 1.1×, 1.2×, 1.3×, 1.4×, 1.5×, 1.6×, 1.7×, 1.8×, 1.9×, 2×, 2.1×, 2.2×, 2.3×, 2.4×, 2.5×, 2.6×, 2.7×, 2.8×, 2.9×, 3×, 3.5×, 4×, 4.5×, 5×, 6×, 7×, 8×, 9×, 10×, 15×, 20×, 30×, 40×, 50×, 100×, 500×, or 1000× compared to the current level.In some cases, the increased expression level of one or more of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A (e.g., 1, 2, 3, 4, 5, 6, or 7 genes selected from CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A) is at least about 1.1-fold, about 1.2-fold, or greater than the reference expression level of the one or more genes. , about 1.3-fold, about 1.4-fold, about 1.5-fold, about 1.6-fold, about 1.7-fold, about 1.8-fold, about 1.9-fold, about 2-fold, about 2.1-fold, about 2.2-fold, about 2.3-fold, about 2.4-fold, about 2.5-fold, about 2.6-fold, about 2.7-fold, about 2.8-fold, about 2.9-fold, about 3-fold, about 3.5-fold, about 4-fold, about 4.5-fold, about 5-fold, about 6-fold, about 7-fold, about 8-fold, about 9-fold, about 10-fold, about 15-fold, about 20-fold, about 30-fold, about 40-fold, about 50-fold, about 100-fold, about 500-fold, or about 1,000-fold or more.

[0121] In certain cases, the methods and assays may include determining an increased expression level of at least two of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A in a sample from an individual compared to a reference expression level of the at least two genes (e.g., an increased expression level of about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or more), identifying the individual as susceptible to disease progression. In certain cases, the increased expression level of at least two of the following genes, CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, is an increase of at least about 1.1×, 1.2×, 1.3×, 1.4×, 1.5×, 1.6×, 1.7×, 1.8×, 1.9×, 2×, 2.1×, 2.2×, 2.3×, 2.4×, 2.5×, 2.6×, 2.7×, 2.8×, 2.9×, 3×, 3.5×, 4×, 4.5×, 5×, 6×, 7×, 8×, 9×, 10×, 15×, 20×, 30×, 40×, 50×, 100×, 500×, or 1000× as compared to a reference expression level of the at least two genes. In some instances, the increased expression level of at least two of the following genes: CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A is at least about 1.1 fold, about 1.2 fold, about 1.3 fold, about 1.4 fold, about 1.5 fold, about 1.6 fold, about 1.7 fold, about 1.8 fold, about 1.9 fold, about 2.0 fold, about 2.1 fold, about 2.2 fold, about 2.3 fold, about 2.4 fold, about 2.5 fold, about 2.6 fold, about 2.7 fold, about 2.8 fold, about 2.9 fold, about 3.0 fold, about 3.1 fold, about 3.2 fold, about 3.3 fold, about 3.4 fold, about 3.5 fold, The increase is about 1.9-fold, about 2-fold, about 2.1-fold, about 2.2-fold, about 2.3-fold, about 2.4-fold, about 2.5-fold, about 2.6-fold, about 2.7-fold, about 2.8-fold, about 2.9-fold, about 3-fold, about 3.5-fold, about 4-fold, about 4.5-fold, about 5-fold, about 6-fold, about 7-fold, about 8-fold, about 9-fold, about 10-fold, about 15-fold, about 20-fold, about 30-fold, about 40-fold, about 50-fold, about 100-fold, about 500-fold, or about 1,000-fold or more. In some instances of the diagnostic method, the expression level of a combination of two genes set forth in Table 1, such as any of the exemplary combinations set forth in Table 4, may be determined. Table 4. Combinations of two genes from CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A TIFF0007652566000005.tif202170

[0122] In certain cases, the methods and assays may include determining an increased expression level of at least three of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A in a sample from an individual compared to a reference expression level of the at least three genes (e.g., an increased expression level of about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or more), identifying the individual as susceptible to disease progression. In certain cases, the increased expression levels of at least three of the following genes, CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, is an increase of at least about 1.1×, 1.2×, 1.3×, 1.4×, 1.5×, 1.6×, 1.7×, 1.8×, 1.9×, 2×, 2.1×, 2.2×, 2.3×, 2.4×, 2.5×, 2.6×, 2.7×, 2.8×, 2.9×, 3×, 3.5×, 4×, 4.5×, 5×, 6×, 7×, 8×, 9×, 10×, 15×, 20×, 30×, 40×, 50×, 100×, 500×, or 1000× as compared to the reference expression levels of the at least three genes. In some instances, the increased expression levels of at least three of the following genes: CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A are at least about 1.1 fold, about 1.2 fold, about 1.3 fold, about 1.4 fold, about 1.5 fold, about 1.6 fold, about 1.7 fold, about 1.8 fold, about 1.9 fold, about 2.0 fold, about 2.1 fold, about 2.2 fold, about 2.3 fold, about 2.4 fold, about 2.5 fold, about 2.6 fold, about 2.7 fold, about 2.8 fold, about 2.9 fold, about 3.0 fold, about 3.1 fold, about 3.2 fold, about 3.3 fold, about 3.4 fold, about 3.5 fold, about 3.6 fold, about 3.7 fold, about 3.8 fold, about 3.9 ...9 fold, about 3.1 fold, about 3.2 fold, about 3.3 fold, about 3.4 fold, about 3.5 fold, about 3.6 fold, about 3.7 fold, The increase is about 1.9-fold, about 2-fold, about 2.1-fold, about 2.2-fold, about 2.3-fold, about 2.4-fold, about 2.5-fold, about 2.6-fold, about 2.7-fold, about 2.8-fold, about 2.9-fold, about 3-fold, about 3.5-fold, about 4-fold, about 4.5-fold, about 5-fold, about 6-fold, about 7-fold, about 8-fold, about 9-fold, about 10-fold, about 15-fold, about 20-fold, about 30-fold, about 40-fold, about 50-fold, about 100-fold, about 500-fold, or about 1,000-fold or more. In some instances of the diagnostic method, the expression level of a combination of three genes set forth in Table 1, such as any of the exemplary combinations set forth in Table 5, may be determined. Table 5. Combinations of three genes among CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A TIFF0007652566000006.tif255170TIFF0007652566000007.tif182170

[0123] In certain cases, the methods and assays may include determining an increased expression level of at least four of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A in a sample from an individual compared to a reference expression level of the at least four genes (e.g., an increased expression level of about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or more), identifying the individual as susceptible to disease progression. In certain cases, the increased expression levels of at least four of the following genes, CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, is an increase of at least about 1.1×, 1.2×, 1.3×, 1.4×, 1.5×, 1.6×, 1.7×, 1.8×, 1.9×, 2×, 2.1×, 2.2×, 2.3×, 2.4×, 2.5×, 2.6×, 2.7×, 2.8×, 2.9×, 3×, 3.5×, 4×, 4.5×, 5×, 6×, 7×, 8×, 9×, 10×, 15×, 20×, 30×, 40×, 50×, 100×, 500×, or 1000× as compared to the reference expression levels of the at least four genes. In some instances, the increased expression levels of at least four of the following genes, CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, are at least about 1.1 fold, about 1.2 fold, about 1.3 fold, about 1.4 fold, about 1.5 fold, about 1.6 fold, about 1.7 fold, about 1.8 fold, about 1.9 fold, about 2.0 fold, about 2.1 fold, about 2.2 fold, about 2.3 fold, about 2.4 fold, about 2.5 fold, about 2.6 fold, about 2.7 fold, about 2.8 fold, about 2.9 fold, about 3.0 fold, about 3.1 fold, about 3.2 fold, about 3.3 fold, about 3.4 fold, about 3.5 fold The increase is about 1.9-fold, about 2-fold, about 2.1-fold, about 2.2-fold, about 2.3-fold, about 2.4-fold, about 2.5-fold, about 2.6-fold, about 2.7-fold, about 2.8-fold, about 2.9-fold, about 3-fold, about 3.5-fold, about 4-fold, about 4.5-fold, about 5-fold, about 6-fold, about 7-fold, about 8-fold, about 9-fold, about 10-fold, about 15-fold, about 20-fold, about 30-fold, about 40-fold, about 50-fold, about 100-fold, about 500-fold, or about 1,000-fold or more. In some instances of the diagnostic method, the expression level of a combination of four genes set forth in Table 1, such as any of the exemplary combinations set forth in Table 6, may be determined. Table 6. Combinations of four genes among CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A TIFF0007652566000008.tif255170TIFF0007652566000009.tif182170

[0124] In certain cases, the methods and assays may include determining an increased expression level of at least five of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A in a sample from an individual compared to a reference expression level of the at least five genes (e.g., an increased expression level of about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or more), identifying the individual as susceptible to disease progression. In certain cases, the increased expression levels of at least five of the following genes, CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, is an increase of at least about 1.1×, 1.2×, 1.3×, 1.4×, 1.5×, 1.6×, 1.7×, 1.8×, 1.9×, 2×, 2.1×, 2.2×, 2.3×, 2.4×, 2.5×, 2.6×, 2.7×, 2.8×, 2.9×, 3×, 3.5×, 4×, 4.5×, 5×, 6×, 7×, 8×, 9×, 10×, 15×, 20×, 30×, 40×, 50×, 100×, 500×, or 1000× as compared to the reference expression levels of the at least five genes. In some instances, the increased expression levels of at least five of the following genes, CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, are at least about 1.1 fold, about 1.2 fold, about 1.3 fold, about 1.4 fold, about 1.5 fold, about 1.6 fold, about 1.7 fold, about 1.8 fold, about 1.9 fold, about 2.0 fold, about 2.1 fold, about 2.2 fold, about 2.3 fold, about 2.4 fold, about 2.5 fold, about 2.6 fold, about 2.7 fold, about 2.8 fold, about 2.9 fold, about 3.0 fold, about 3.1 fold, about 3.2 fold, about 3.3 fold, about 3.4 fold, about 3.5 fold The increase is about 1.9-fold, about 2-fold, about 2.1-fold, about 2.2-fold, about 2.3-fold, about 2.4-fold, about 2.5-fold, about 2.6-fold, about 2.7-fold, about 2.8-fold, about 2.9-fold, about 3-fold, about 3.5-fold, about 4-fold, about 4.5-fold, about 5-fold, about 6-fold, about 7-fold, about 8-fold, about 9-fold, about 10-fold, about 15-fold, about 20-fold, about 30-fold, about 40-fold, about 50-fold, about 100-fold, about 500-fold, or about 1,000-fold or more. In some instances of the diagnostic method, the expression level of a combination of five genes set forth in Table 1, such as any of the exemplary combinations set forth in Table 7, may be determined. Table 7. Combinations of five genes among CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A TIFF0007652566000010.tif255170

[0125] In certain cases, the methods and assays may include determining an increased expression level of at least six of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A in a sample compared to a reference expression level of the at least six genes (e.g., an increased expression level of about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or more), identifying the individual as susceptible to disease progression. In certain cases, the increased expression levels of at least six of the following genes, CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, is an increase of at least about 1.1×, 1.2×, 1.3×, 1.4×, 1.5×, 1.6×, 1.7×, 1.8×, 1.9×, 2×, 2.1×, 2.2×, 2.3×, 2.4×, 2.5×, 2.6×, 2.7×, 2.8×, 2.9×, 3×, 3.5×, 4×, 4.5×, 5×, 6×, 7×, 8×, 9×, 10×, 15×, 20×, 30×, 40×, 50×, 100×, 500×, or 1000× as compared to the reference expression levels of the at least six genes. In some instances, the increased expression levels of at least six of the following genes, CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, are at least about 1.1 fold, about 1.2 fold, about 1.3 fold, about 1.4 fold, about 1.5 fold, about 1.6 fold, about 1.7 fold, about 1.8 fold, about 1.9 fold, about 2.0 fold, about 2.1 fold, about 2.2 fold, about 2.3 fold, about 2.4 fold, about 2.5 fold, about 2.6 fold, about 2.7 fold, about 2.8 fold, about 2.9 fold, about 3.0 fold, about 3.1 fold, about 3.2 fold, about 3.3 fold, about 3.4 fold, about 3.5 fold The increase is about 1.9-fold, about 2-fold, about 2.1-fold, about 2.2-fold, about 2.3-fold, about 2.4-fold, about 2.5-fold, about 2.6-fold, about 2.7-fold, about 2.8-fold, about 2.9-fold, about 3-fold, about 3.5-fold, about 4-fold, about 4.5-fold, about 5-fold, about 6-fold, about 7-fold, about 8-fold, about 9-fold, about 10-fold, about 15-fold, about 20-fold, about 30-fold, about 40-fold, about 50-fold, about 100-fold, about 500-fold, or about 1,000-fold or more. In some instances of the diagnostic method, the expression level of a combination of six genes set forth in Table 1, such as any of the exemplary combinations set forth in Table 8, may be determined. Table 8. Combinations of six genes among CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A TIFF0007652566000011.tif91170

[0126] In certain cases, the methods and assays may include determining an increased expression level of the following seven genes in a sample, namely CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, compared to a reference expression level of the seven genes (e.g., an increased expression level of about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or more), identifying the individual as susceptible to disease progression. In certain cases, the increased expression levels of the following seven genes, namely CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, is an increase of at least about 1.1x, 1.2x, 1.3x, 1.4x, 1.5x, 1.6x, 1.7x, 1.8x, 1.9x, 2x, 2.1x, 2.2x, 2.3x, 2.4x, 2.5x, 2.6x, 2.7x, 2.8x, 2.9x, 3x, 3.5x, 4x, 4.5x, 5x, 6x, 7x, 8x, 9x, 10x, 15x, 20x, 30x, 40x, 50x, 100x, 500x, or 1000x compared to the reference expression levels of the seven genes. In some cases, the increased expression levels of the following seven genes, namely CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, are at least about 1.1 fold, about 1.2 fold, about 1.3 fold, about 1.4 fold, about 1.5 fold, about 1.6 fold, about 1.7 fold, about 1.8 fold, about 1.9 fold, ...8 fold, about 1.9 fold, about 1.1 fold, about 1.2 fold, about 1.3 fold, about 1.4 fold, about 1.5 fold, about 1.6 fold, about 1.7 fold, about 1.8 fold, about 1.9 fold, about 1.8 fold, about 1.9 fold, about 1.1 fold, about 1.2 fold, about 1.3 fold, about 1.4 fold, about 1.5 fold, about 1.6 fold, about 1.7 fold, about 1.8 fold, An increase of 2-fold, about 2.1-fold, about 2.2-fold, about 2.3-fold, about 2.4-fold, about 2.5-fold, about 2.6-fold, about 2.7-fold, about 2.8-fold, about 2.9-fold, about 3-fold, about 3.5-fold, about 4-fold, about 4.5-fold, about 5-fold, about 6-fold, about 7-fold, about 8-fold, about 9-fold, about 10-fold, about 15-fold, about 20-fold, about 30-fold, about 40-fold, about 50-fold, about 100-fold, about 500-fold, or about 1,000-fold or more.

[0127] In some cases, the methods and assays provided herein may involve determining the expression level of one or more genes set forth in Table 3 (e.g., one, two, three, four, five, or six genes set forth in Table 3) in a sample from an individual. For example, the method or assay may include determining the expression level of one or more genes set forth in Table 3, where a decrease in the expression level of one or more genes set forth in Table 3 compared to a reference expression level (e.g., a decrease of about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or more in the expression level) identifies the individual as likely to exhibit disease progression. In certain cases, the decreased expression level of one or more genes listed in Table 3 (e.g., 1, 2, 3, 4, 5, or 6 genes listed in Table 3) is at least about a 1.1x, 1.2x, 1.3x, 1.4x, 1.5x, 1.6x, 1.7x, 1.8x, 1.9x, 2x, 2.1x, 2.2x, 2.3x, 2.4x, 2.5x, 2.6x, 2.7x, 2.8x, 2.9x, 3x, 3.5x, 4x, 4.5x, 5x, 6x, 7x, 8x, 9x, 10x, 15x, 20x, 30x, 40x, 50x, 100x, 500x, or 1000x decrease compared to the reference expression level of the one or more genes. In some cases, the decreased expression level of one or more genes listed in Table 3 (e.g., 1, 2, 3, 4, 5, or 6 genes listed in Table 3) is at least about 1.1-fold, about 1.2-fold, about 1.3-fold, about 1.4-fold, about 1.5-fold, about 1.6-fold, about 1.7-fold, about 1.8-fold, about 1.9-fold, or about 1.8-fold relative to the reference expression level of the one or more genes. , about 2-fold, about 2.1-fold, about 2.2-fold, about 2.3-fold, about 2.4-fold, about 2.5-fold, about 2.6-fold, about 2.7-fold, about 2.8-fold, about 2.9-fold, about 3-fold, about 3.5-fold, about 4-fold, about 4.5-fold, about 5-fold, about 6-fold, about 7-fold, about 8-fold, about 9-fold, about 10-fold, about 15-fold, about 20-fold, about 30-fold, about 40-fold, about 50-fold, about 100-fold, about 500-fold, or about 1,000-fold or more.

[0128] In any of the above-mentioned prognostic methods and assays, the reference expression level can be a reference expression level in a reference population of individuals with RA who have not been previously treated with DMARDs, the population of individuals being comprised of a first subset of individuals who have disease progression and a second subset of individuals who have not disease progression.In some cases, the reference expression level significantly separates each of the first and second subsets of individuals based on the significant difference in the expression level of one or more genes listed in Table 1, Table 2 or Table 3 in the first subset of individuals compared with that of the second subset of individuals.In some cases, after about 12 months, the first subset of individuals has disease progression and the second subset of individuals has not disease progression.

[0129] In any of the above-mentioned prognostic methods and assays, in which the expression level of one or more biomarkers selected from the biomarkers listed in Table 1, Table 2, or Table 3 is determined in a sample from an individual and compared to a reference expression level (e.g., a pre-assigned expression level of one or more genes listed in Table 1, Table 2, or Table 3), it is understood that in some cases, the expression level of one or more biomarkers may be the average expression level of the one or more biomarkers. In some cases, the expression level of one or more biomarkers may be the median expression level of the one or more biomarkers. In some cases, the expression level of one or more biomarkers may be normalized to, for example, a reference gene, e.g., a housekeeping gene. In some cases, the reference gene is ACTB, GAPDH, GUSB, HPRT1, PGK1, RPL19, TUBB, or TMEM55B. In some cases, the expression level of one or more biomarkers may be the average normalized expression level of the one or more biomarkers. In some cases, the expression level of one or more biomarkers may be the median normalized expression level of the one or more biomarkers.

[0130] In any of the above-mentioned prognostic methods and assays in which the expression levels of more than one biomarker selected from the genes listed in Table 1, Table 2, or Table 3 are determined in a sample from an individual and compared to a reference expression level (e.g., a pre-assigned expression level of one or more genes listed in Table 1, Table 2, or Table 3), it is understood that in some cases, the expression level of each individual biomarker in the sample is compared to the reference expression level of each individual biomarker. For example, when the expression levels of CD180 and CXCL1 are determined in a sample from an individual and compared to the reference expression levels of CD180 and CXCL1, in some cases, the expression level of CD180 in the sample from the individual is compared to the reference expression level of CD180, and the expression level of CXCL1 in the sample from the individual is compared to the reference expression level of CXCL1. In other cases, the expression levels of more than one gene of interest may be determined by counting methods known to those of skill in the art and disclosed herein, including, for example, calculating the median or mean of all the expression levels of the genes of interest. Prior to counting, the expression level of each gene of interest may be normalized by using statistical methods known to those skilled in the art and disclosed herein, including, for example, normalizing to the expression level of one or more housekeeping genes, or normalizing to the total library size, or normalizing to the median or average expression level across all genes measured. In some cases, prior to counting across multiple genes of interest, the normalized expression level of each gene of interest may be standardized by using statistical methods known to those skilled in the art and disclosed herein, including, for example, calculating the Z-score of the normalized expression level of each gene of interest.

[0131] In any of the above-mentioned prognostic methods and assays, disease progression can be radiographic progression. Radiographic progression can be characterized by an increase in ShSS (e.g., an increase in ShSS over a defined time frame).

[0132] The present invention also provides methods and assays that can be used to identify individuals with RA who may be likely to exhibit disease activity, involving determining a myeloid intrinsic gene score from a sample from the individual, where a myeloid intrinsic gene score from the sample that is equal to or greater than a reference myeloid intrinsic gene score identifies the individual as one who may be likely to exhibit disease activity. In some cases, the methods and assays provided herein may further involve determining a lymphoid intrinsic gene score from a sample from the individual, where a lymphoid intrinsic gene score from the sample that is equal to or greater than a reference lymphoid intrinsic gene score identifies the individual as one who may be likely to exhibit disease activity.

[0133] The present invention provides methods and assays that can be used to identify individuals with RA who may be unlikely to exhibit disease activity, which involve determining a minor immune-fibrotic intrinsic gene score from a sample from the individual, where a minor immune-fibrotic intrinsic gene score from the sample that is equal to or greater than a reference minor immune-fibrotic intrinsic gene score identifies the individual as one who may be unlikely to exhibit disease activity.

[0134] In any of the above-mentioned prognostic methods and assays, the reference myeloid intrinsic gene score may be a reference myeloid intrinsic gene score in a reference population of individuals with RA, the population of individuals consisting of a first subset of individuals who exhibit disease activity and a second subset of individuals who do not exhibit disease activity. In some cases, the reference myeloid intrinsic gene score level significantly separates each of the first and second subsets of individuals based on a significant difference in the myeloid intrinsic gene score of the first subset of individuals compared to that of the second subset of individuals.

[0135] In any of the above-mentioned prognostic methods and assays, the reference lymphoid-specific gene score may be a reference lymphoid-specific gene score in a reference population of individuals with RA, the population of individuals consisting of a first subset of individuals who have disease activity and a second subset of individuals who have not disease activity. In some cases, the reference lymphoid-specific gene score level significantly separates each of the first and second subsets of individuals based on a significant difference in the lymphoid-specific gene score of the first subset of individuals compared to that of the second subset of individuals.

[0136] In any of the above-mentioned prognostic methods and assays, the reference minor immune-fibrotic intrinsic gene score may be a reference minor immune-fibrotic intrinsic gene score in a reference population of individuals with RA, the population of individuals consisting of a first subset of individuals who exhibit disease activity and a second subset of individuals who do not exhibit disease activity. In some cases, the reference minor immune-fibrotic intrinsic gene score level significantly separates each of the first and second subsets of individuals based on a significant difference in the minor immune-fibrotic intrinsic gene score of the first minor immune-fibrotic subset compared to that of the second subset of individuals.

[0137] In any of the above-mentioned prognostic methods and assays, the severity of disease activity may be assessed, for example, by evaluating ACR and / or EULAR clinical indices including, but not limited to, DAS28-ESR, DAS28-CRP, ESR levels, CRP levels, ACPA titers, assessment of joint damage (e.g., by x-ray, ultrasound synovial thickening (USST) assessment, or ultrasound power Doppler (USPD) score), or combinations thereof.

[0138] In any of the above-mentioned prognostic methods and assays, the methods and assays may further comprise determining one or more clinical covariates of the individual (e.g., baseline RF titer, disease duration, DAS28-ESR, DAS28-CRP, baseline pathology, and USST and USPD 12 max scores).

[0139] In any of the above-mentioned prognostic methods and assays, the methods and assays may further comprise administering to the individual a therapeutic agent other than or in addition to a disease-modifying antirheumatic drug (DMARD) (e.g., as described in Section II-B below). In certain cases, when the change in the expression level of one or more genes listed in Table 1 or Table 2 compared to the reference expression level is an increase, the method further comprises administering to the individual a therapeutic agent other than or in addition to a disease-modifying antirheumatic drug (DMARD). In certain cases, when the change in the expression level of one or more genes listed in Table 3 compared to the reference expression level is a decrease, the method further comprises administering to the individual a therapeutic agent other than or in addition to a disease-modifying antirheumatic drug (DMARD).

[0140] In some cases of any of the above-mentioned prior methods and assays, the RA therapeutic agent may be a DMARD (e.g., as described in Section II-B below). In some cases of any of the above-mentioned prior methods, the RA therapeutic agent may be a therapeutic agent other than a DMARD (e.g., as described in Section II-B below).

[0141] In some cases, the individual has not been previously treated with a DMARD. In other cases, the individual has been previously treated with a DMARD.

[0142] (ii) Predictive Diagnostic Methods and Assays The present invention provides methods and assays for identifying individuals with RA who may benefit from treatment with RA therapeutics, including DMARDs (e.g., methotrexate), involving determining a myeloid intrinsic gene score from a sample from the individual, where a myeloid intrinsic gene score from the sample that is equal to or greater than a reference myeloid intrinsic gene score (e.g., an increase of about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or more above the myeloid intrinsic gene score) identifies the individual as one who may benefit from treatment with a DMARD. In some cases, the methods and assays provided herein may further involve determining a lymphoid-specific gene score from a sample from the individual, where a lymphoid-specific gene score from the sample that is equal to or greater than a reference lymphoid-specific gene score (e.g., greater than or about a 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or greater increase therebetween) identifies the individual as one who may benefit from treatment with a DMARD.

[0143] The present invention also provides methods and assays for selecting a therapy for an individual with RA, the methods or assays comprising determining a myeloid intrinsic gene score from a sample from the individual, wherein a myeloid intrinsic gene score from the sample that is equal to or greater than a reference myeloid intrinsic gene score (e.g., greater than or about a 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or greater increase therebetween) identifies the individual as one who may benefit from treatment including a DMARD (e.g., methotrexate). In some cases, a myeloid-specific gene score above a reference myeloid-specific gene score, i.e., an elevated or increased myeloid-specific gene score, refers to an overall increase of at least about 1.1×, 1.2×, 1.3×, 1.4×, 1.5×, 1.6×, 1.7×, 1.8×, 1.9×, 2×, 2.1×, 2.2×, 2.3×, 2.4×, 2.5×, 2.6×, 2.7×, 2.8×, 2.9×, 3×, 3.5×, 4×, 4.5×, 5×, 6×, 7×, 8×, 9×, 10×, 15×, 20×, 30×, 40×, 50×, 100×, 500×, or 1000× as compared to a reference myeloid-specific gene score. In some cases, an elevated myeloid-specific gene score refers to an overall increase in the myeloid-specific gene score of more than about 1.1 fold, about 1.2 fold, about 1.3 fold, about 1.4 fold, about 1.5 fold, about 1.6 fold, about 1.7 fold, about 1.8 fold, about 1.9 fold, about 2 fold, about 2.1 fold, about 2.2 fold, about 2.3 fold, about 2.4 fold, about 2.5 fold, about 2.6 fold, about 2.7 fold, about 2.8 fold, about 2.9 fold, about 3 fold, about 3.5 fold, about 4 fold, about 4.5 fold, about 5 fold, about 6 fold, about 7 fold, about 8 fold, about 9 fold, about 10 fold, about 15 fold, about 20 fold, about 30 fold, about 40 fold, about 50 fold, about 100 fold, about 500 fold, about 1,000 fold or more as compared to a reference myeloid-specific gene score.In some cases, the methods and assays may further involve determining a lymphoid-specific gene score from a sample from the individual, where a lymphoid-specific gene score from the sample that is equal to or greater than a reference lymphoid-specific gene score (e.g., greater than or about a 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or greater increase therebetween) identifies the individual as one who may benefit from treatment that includes a DMARD. In some cases, a lymphoid-specific gene score above a reference lymphoid-specific gene score, i.e., an elevated or increased lymphoid-specific gene score, refers to an overall increase of at least about 1.1×, 1.2×, 1.3×, 1.4×, 1.5×, 1.6×, 1.7×, 1.8×, 1.9×, 2×, 2.1×, 2.2×, 2.3×, 2.4×, 2.5×, 2.6×, 2.7×, 2.8×, 2.9×, 3×, 3.5×, 4×, 4.5×, 5×, 6×, 7×, 8×, 9×, 10×, 15×, 20×, 30×, 40×, 50×, 100×, 500×, or 1000× as compared to the reference lymphoid-specific gene score. In some cases, an elevated lymphoid-specific gene score refers to an overall increase in the lymphoid-specific gene score of more than about 1.1 fold, about 1.2 fold, about 1.3 fold, about 1.4 fold, about 1.5 fold, about 1.6 fold, about 1.7 fold, about 1.8 fold, about 1.9 fold, about 2 fold, about 2.1 fold, about 2.2 fold, about 2.3 fold, about 2.4 fold, about 2.5 fold, about 2.6 fold, about 2.7 fold, about 2.8 fold, about 2.9 fold, about 3 fold, about 3.5 fold, about 4 fold, about 4.5 fold, about 5 fold, about 6 fold, about 7 fold, about 8 fold, about 9 fold, about 10 fold, about 15 fold, about 20 fold, about 30 fold, about 40 fold, about 50 fold, about 100 fold, about 500 fold, about 1,000 fold or more as compared to a reference lymphoid-specific gene score.

[0144] In any of the above-mentioned prediction methods and assays, the reference myeloid intrinsic gene score can be a reference myeloid intrinsic gene score in a reference population of individuals with RA who have been previously treated with DMARDs, the population of individuals being composed of a first subset of individuals who responded to DMARD therapy and a second subset of individuals who did not respond to DMARD therapy. In some cases, the reference myeloid intrinsic gene score significantly separates each of the first and second subsets of individuals based on a significant difference between the myeloid intrinsic gene score of the first subset of individuals compared to that of the second subset of individuals. In some cases, about 6 months after the start of DMARD therapy, the first subset of individuals responded to DMARD therapy and the second subset did not respond to DMARD therapy.

[0145] In any of the above-mentioned prediction methods and assays, the reference lymphoid intrinsic gene score can be a reference lymphoid intrinsic gene score in a reference population of individuals with RA who have been previously treated with DMARDs, the population of individuals being composed of a first subset of individuals who responded to DMARD therapy and a second subset of individuals who did not respond to DMARD therapy.In some cases, the reference lymphoid intrinsic gene score significantly separates each of the first and second subsets of individuals based on the significant difference between the lymphoid intrinsic gene score of the first subset of individuals compared to that of the second subset of individuals.In some cases, about 6 months after the start of DMARD therapy, the first subset of individuals responded to DMARD therapy and the second subset did not respond to DMARD therapy.

[0146] The present invention provides methods and assays that may be used to monitor the response of an individual with RA to treatment with a DMARD, the methods or assays comprising: (i) determining a myeloid intrinsic gene score from a sample from the individual at a first time point during or after administration of a DMARD; (ii) determining a second myeloid intrinsic gene score from a sample from the individual at a second time point; and (iii) comparing the first myeloid intrinsic gene score to the second myeloid intrinsic gene score, wherein a decrease in the second myeloid intrinsic gene score compared to the first myeloid intrinsic gene score (e.g., about a 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or greater decrease in the myeloid intrinsic gene score) is predictive of an individual likely to respond to treatment with a DMARD. The methods and assays may further involve (i) determining a lymphoid-specific gene score from a sample from the individual at a first time point during or after administration of a DMARD; (ii) determining a second lymphoid-specific gene score from a sample from the individual at a second time point; and (iii) comparing the first lymphoid-specific gene score to the second lymphoid-specific gene score, where a decrease in the second lymphoid-specific gene score compared to the first lymphoid-specific gene score (e.g., about a 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or more decrease in the lymphoid-specific gene score) is further predictive of the individual being likely to respond to treatment with a DMARD. In some cases, a reduced or decreased myeloid intrinsic gene score refers to an overall decrease of at least about 1.1×, 1.2×, 1.3×, 1.4×, 1.5×, 1.6×, 1.7×, 1.8×, 1.9×, 2×, 2.1×, 2.2×, 2.3×, 2.4×, 2.5×, 2.6×, 2.7×, 2.8×, 2.9×, 3×, 3.5×, 4×, 4.5×, 5×, 6×, 7×, 8×, 9×, 10×, 15×, 20×, 30×, 40×, 50×, 100×, 500×, or 1000× as compared to a reference myeloid intrinsic gene score.In some cases, a reduced myeloid intrinsic gene score refers to an overall decrease in the myeloid intrinsic gene score of more than about 1.1 fold, about 1.2 fold, about 1.3 fold, about 1.4 fold, about 1.5 fold, about 1.6 fold, about 1.7 fold, about 1.8 fold, about 1.9 fold, about 2 fold, about 2.1 fold, about 2.2 fold, about 2.3 fold, about 2.4 fold, about 2.5 fold, about 2.6 fold, about 2.7 fold, about 2.8 fold, about 2.9 fold, about 3 fold, about 3.5 fold, about 4 fold, about 4.5 fold, about 5 fold, about 6 fold, about 7 fold, about 8 fold, about 9 fold, about 10 fold, about 15 fold, about 20 fold, about 30 fold, about 40 fold, about 50 fold, about 100 fold, about 500 fold, about 1,000 fold or more as compared to a reference myeloid intrinsic gene score. In some cases, a reduced or decreased lymphoid-specific gene score refers to an overall decrease of at least about 1.1×, 1.2×, 1.3×, 1.4×, 1.5×, 1.6×, 1.7×, 1.8×, 1.9×, 2×, 2.1×, 2.2×, 2.3×, 2.4×, 2.5×, 2.6×, 2.7×, 2.8×, 2.9×, 3×, 3.5×, 4×, 4.5×, 5×, 6×, 7×, 8×, 9×, 10×, 15×, 20×, 30×, 40×, 50×, 100×, 500×, or 1000× as compared to a reference lymphoid-specific gene score. In some cases, a reduced lymphoid-specific gene score refers to an overall decrease in the lymphoid-specific gene score of more than about 1.1 fold, about 1.2 fold, about 1.3 fold, about 1.4 fold, about 1.5 fold, about 1.6 fold, about 1.7 fold, about 1.8 fold, about 1.9 fold, about 2 fold, about 2.1 fold, about 2.2 fold, about 2.3 fold, about 2.4 fold, about 2.5 fold, about 2.6 fold, about 2.7 fold, about 2.8 fold, about 2.9 fold, about 3 fold, about 3.5 fold, about 4 fold, about 4.5 fold, about 5 fold, about 6 fold, about 7 fold, about 8 fold, about 9 fold, about 10 fold, about 15 fold, about 20 fold, about 30 fold, about 40 fold, about 50 fold, about 100 fold, about 500 fold, about 1,000 fold or more as compared to a reference lymphoid-specific gene score.

[0147] In some cases, the second myeloid-specific gene score is decreased compared to the first myeloid-specific gene score, and the method or assay further comprises administering to the individual an additional dose of a DMARD. In some cases, the second lymphoid-specific gene score is decreased compared to the first lymphoid-specific gene score, and the method or assay further comprises administering to the individual an additional dose of a DMARD.

[0148] In some cases, the second myeloid intrinsic gene score is decreased compared to the first myeloid intrinsic gene score and the second lymphoid intrinsic gene score is decreased compared to the first lymphoid intrinsic gene score, and the method or assay further involves administering to the individual an additional dose of a DMARD.

[0149] In some cases of any of the preceding methods or assays involving determining a myeloid intrinsic gene score and / or a lymphoid intrinsic gene score, the individual has been previously treated with a DMARD. In other cases, the individual has not been previously treated with a DMARD.

[0150] iii. Exemplary Strategies for Determining Biomarker Expression Levels The methods and assays provided herein may include determining the expression level of one or more genes in a sample from an individual (e.g., a synovial tissue sample, a synovial fluid sample, or a combination thereof). The sample from an individual may be an archived sample, a fresh sample, or a frozen sample. The expression level of one or more genes may be determined qualitatively and / or quantitatively based on any suitable criteria known in the art, including, but not limited to, measuring DNA, mRNA, cDNA, protein, protein fragment, and / or gene copy number levels in the individual. Techniques for measuring such biomarkers are known in the art and understood by the skilled artisan, and include whole genome sequencing, polymerase chain reaction (PCR), including quantitative real-time PCR (qRT-PCR), and other amplification-based detection methods, such as branched DNA, SISBA, TMA, etc., RNASeq, microarray analysis, gene expression profiling, whole genome sequencing (WGS), and / or serial analysis of gene expression ("SAGE"), direct digital counting of nucleic acids (e.g., Nanostring counting). These include, but are not limited to, any one of a wide variety of assays that can be performed by ELISA, ELIFA, fluorescence activated cell sorting ("FACS"), MassARRAY, proteomics, biochemical enzyme activity assays, in situ hybridization (ISH), fluorescent in situ hybridization (FISH), Southern analysis, Northern analysis, and protein, gene, and / or tissue array analysis. Exemplary protocols for assessing the status of genes and gene products can be found, for example, in Ausubel et al. eds. (Current Protocols In Molecular Biology, 1995), Units 2 (Northern Blotting), 4 (Southern Blotting), 15 (Immunoblotting), and 18 (PCR Analysis).Multiplexed immunoassays such as those available from Rules Based Medicine or Meso Scale Discovery ("MSD") may also be used.

[0151] In some cases of any of the preceding methods and assays, the expression level of the biomarker may be a nucleic acid expression level (e.g., a DNA expression level or an RNA expression level (e.g., an mRNA expression level)). Any suitable method of determining the expression level of the nucleic acid may be used. In some cases, the expression level of the nucleic acid is determined using direct digital counting of the nucleic acid (e.g., Nanostring nCounter), RNAseq, RT-qPCR, qPCR, multiplex qPCR or RT-qPCR, microarray analysis, SAGE, MassARRAY technology, ISH, or a combination thereof.

[0152] Methods for evaluating mRNA in cells are well known and include, for example, serial analysis of gene expression (SAGE), whole genome sequencing (WGS), hybridization assays using complementary DNA probes (such as in situ hybridization, Northern blots, and related techniques using labeled riboprobes specific for one or more genes of interest), and various nucleic acid amplification assays (such as RT-PCR (e.g., qRT-PCR) using complementary primers specific for one or more of the genes of interest, and other amplification-based detection methods such as branched DNA, SISBA, TMA, etc.). In addition, such methods can include one or more steps that allow the level of the target mRNA to be determined in a biological sample (e.g., by simultaneously examining the level of a control mRNA sequence of a "housekeeping" gene such as an actin family member). Optionally, the sequence of the amplified target cDNA can be determined. Optional methods include protocols for examining or detecting mRNA, such as target mRNA, in tissue or cell samples by microarray technology. Using nucleic acid microarrays, test and control mRNA samples from test and control tissue samples are reverse transcribed and labeled to generate cDNA probes. The probes are then hybridized to an array of nucleic acids immobilized on a solid support. The array is constructed such that the sequence and location of each member of the array is known. For example, selected genes whose expression correlates with increased or decreased clinical benefit of treatments including immunotherapy and inhibitory stromal antagonists may be arrayed on the solid support. Hybridization of a labeled probe with a particular array member indicates that the sample from which the probe was derived expresses that gene.

[0153] In other cases of any of the preceding methods, the expression level of the biomarker may be the expression level of a protein. In certain cases, the method includes contacting the sample with an antibody that specifically binds to a biomarker described herein under conditions that allow binding of the biomarker, and detecting whether a complex is formed between the antibody and the biomarker.

[0154] Any method of measuring the expression level of a protein known in the art or provided herein may be used. For example, in some cases, the expression level of a protein of a biomarker is determined using a method selected from, but not limited to, Western blot, enzyme-linked immunosorbent assay (ELISA), immunoprecipitation, immunohistochemistry (IHC), flow cytometry (e.g., fluorescence activated cell sorting (FACS™)), immunofluorescence, radioimmunoassay, dot blotting, immunodetection, HPLC, surface plasmon resonance, optical spectroscopy, mass spectrometry, liquid chromatography-mass spectrometry (LC-MS), turbidimetry, aptamer technology, and HPLC.

[0155] In certain cases, the presence and / or expression level / amount of biomarker protein in a sample is examined using IHC and staining protocols. IHC staining of tissue sections has been shown to be a reliable method of determining or detecting the presence of a protein in a sample. In some cases of any of the present methods, assays, and / or kits, the biomarker is one or more of the protein expression products of the genes listed in Table 1. In one case, the expression level of the biomarker is determined using a method that includes (a) performing an IHC analysis of a sample (such as a synovial tissue sample obtained from an individual) with an antibody, and (b) determining the expression level of the biomarker in the sample. In some cases, the IHC staining intensity is determined relative to a reference. In some cases, the reference is a reference value. In some cases, the reference is a reference sample (e.g., a control cell line stained sample, a tissue sample from a non-RA affected individual, or a synovial tissue sample that has been determined to be negative for the biomarker of interest).

[0156] IHC may be performed in combination with additional techniques such as morphological staining and / or in situ hybridization (e.g., ISH). Two general methods of IHC are available: direct and indirect assays. According to the first assay, the binding of the antibody to the target antigen is determined directly. This direct assay uses a labeling reagent, such as a fluorescent tag or an enzyme-labeled primary antibody, which can be visualized without further antibody interaction. In a typical indirect assay, an unconjugated primary antibody binds to the antigen, and then a labeled secondary antibody binds to the primary antibody. If the secondary antibody is conjugated to an enzyme label, a chromogenic or fluorogenic substrate is added to provide visualization of the antigen. Signal amplification occurs because several secondary antibodies may react with different epitopes on the primary antibody.

[0157] The primary and / or secondary antibodies used in IHC will typically be labeled with a detectable moiety. Numerous labels are available that can be broadly grouped into the following categories: (a) radioisotopes, e.g. 35 S, 14 C. 125 1. 3 H, and 131(b) colloidal gold particles; (c) fluorescent labels, including, but not limited to, rare earth chelates (europium chelates), Texas Red, rhodamine, fluorescein, dansyl, lissamine, umbelliferone, phycoglycerin, phycoerythrin, phycocyanin, or commercially available fluorophores such as SPECTRUM ORANGE7 and SPECTRUM GREEN7, and / or derivatives of any one or more of the above; (d) a variety of enzyme-substrate labels are available, and U.S. Pat. No. 4,275,149 provides a review of some of these. Examples of enzyme labels include luciferases (e.g., firefly luciferase and bacterial luciferase, see, e.g., U.S. Pat. No. 4,737,456), luciferin, 2,3-dihydrophthalazinedione, malate dehydrogenase, urease, peroxidases such as horseradish peroxidase (HRPO), alkaline phosphatase, β-galactosidase, glucoamylase, lysozyme, carbohydrate oxidases (e.g., glucose oxidase, galactose oxidase, and glucose-6-phosphate dehydrogenase), heterocyclic oxidases (such as uricase and xanthine oxidase), lactoperoxidase, microperoxidase, and the like.

[0158] Examples of enzyme-substrate combinations include, for example, horseradish peroxidase (HRPO) with hydrogen peroxidase as a substrate, alkaline phosphatase (AP) with paranitrophenyl phosphate as a chromogenic substrate, and β-D-galactosidase (β-D-Gal) with a chromogenic (e.g., p-nitrophenyl-β-D-galactosidase) or fluorogenic (e.g., 4-methylumbelliferyl-β-D-galactosidase) substrate. For a general review, see, for example, U.S. Patent Nos. 4,275,149 and 4,318,980.

[0159] Specimens may be prepared, for example, manually or using an automatic staining instrument (for example, Ventana BenchMark XT or Benchmark ULTRA instrument). The specimens thus prepared may be mounted and cover slipped. Slide evaluation may then be determined, for example, using a microscope, and staining intensity criteria may be employed as is commonly used in the art. In some cases, the presence of the biomarker is detected by IHC in >0% of samples, at least 1% of samples, at least 5% of samples, at least 10% of samples, at least 15% of samples, at least 20% of samples, at least 25% of samples, at least 30% of samples, at least 35% of samples, at least 40% of samples, at least 45% of samples, at least 50% of samples, at least 55% of samples, at least 60% of samples, at least 65% of samples, at least 70% of samples, at least 75% of samples, at least 80% of samples, at least 85% of samples, at least 90% of samples, at least 95% of samples, or more. Samples can be scored using any method known in the art, for example, by a pathologist or by automated image analysis.

[0160] In some cases of any of the present methods and assays, the biomarker is detected by immunohistochemistry using a diagnostic antibody (e.g., a primary diagnostic antibody). In some cases, the diagnostic antibody specifically binds to a human antigen. In some cases, the diagnostic antibody is a non-human antibody. In some cases, the diagnostic antibody is a rat, mouse, or rabbit antibody. In some cases, the diagnostic antibody is a rabbit antibody. In some cases, the diagnostic antibody is a monoclonal antibody. In some cases, the diagnostic antibody is directly labeled. In other cases, the diagnostic antibody is indirectly labeled (e.g., by a secondary antibody).

[0161] In some cases of any of the preceding methods and assays, the sample is obtained from the individual prior to (e.g., minutes, hours, days, weeks (e.g., 1, 2, 3, 4, 5, 6, or 7 weeks), months, or years) administration of the RA therapeutic agent. In some cases of any of the preceding methods, the sample from the individual is obtained about 2 to about 10 weeks (e.g., 2, 3, 4, 5, 6, 7, 8, 9, or 10 weeks) after administration of the RA therapeutic agent. In some cases, the sample from the individual is obtained about 4 to about 6 weeks after administration of the RA therapeutic agent.

[0162] In some cases of any of the preceding methods and assays, the expression level or number of biomarkers is detected in a synovial tissue sample, a synovial fluid sample, a primary or cultured cell or cell line, a cell supernatant, a cell lysate, a platelet, a serum, a plasma, a vitreous fluid, a lymphatic fluid, a follicular fluid, a semen, an amniotic fluid, a milk, a whole blood, a cell derived from blood, a urine, a cerebrospinal fluid, a saliva, a sputum, a tear, a sweat, a mucus, a tissue culture medium, a tissue extract, such as a homogenized tissue, a cell extract, or any combination thereof. In some cases, the sample is a tissue sample (e.g., a synovial tissue sample), a cell sample, a whole blood sample, a plasma sample, a serum sample, or a combination thereof. In some cases, the sample is a synovial tissue sample, wherein the synovial tissue sample comprises resident cells, synovial infiltrating immune cells, or a combination thereof. In some cases, the synovial tissue sample is a formalin-fixed and paraffin-embedded (FFPE) sample, an archived sample, a fresh sample, or a frozen sample.

[0163] For example, in some cases of any of the preceding methods and assays, the expression level of a biomarker in a sample (e.g., a synovial tissue sample) is detected in synovial infiltrating immune cells, synovial cells, PBMCs, or a combination thereof using known techniques (e.g., flow cytometry or IHC). Synovial infiltrating immune cells include, but are not limited to, T lymphocytes, B lymphocytes (including plasma cells), or other myeloid lineage cells, including granulocytes (e.g., mast cells), monocytes, macrophages, dendritic cells, and natural killer (NK) cells. In some cases, staining for the biomarker is detected as membrane staining, cytoplasmic staining, or a combination thereof. In other cases, the absence of a biomarker is detected as the absence or no staining in the sample compared to a reference sample.

[0164] In certain cases, reference sample, reference cell, reference tissue, control sample, control cell or control tissue is a single sample or a combination of multiple samples from the same patient or individual, which are obtained at one or more time points different from the time point at which test sample is obtained.For example, reference sample, reference cell, reference tissue, control sample, control cell or control tissue is obtained from the same patient or individual at an earlier time point than the time point at which test sample is obtained.Such reference sample, reference cell, reference tissue, control sample, control cell or control tissue can be useful when reference sample is obtained during the initial diagnosis of RA, and test sample is obtained later when disease progresses.

[0165] In certain cases, the reference sample, reference cell, reference tissue, control sample, control cell, or control tissue is a combined multiple sample from one or more healthy individuals that are not the patient. In certain cases, the reference sample, reference cell, reference tissue, control sample, control cell, or control tissue is a combined multiple sample from one or more individuals that are not the patient or individual and have a disease or disorder (e.g., RA). In certain cases, the reference sample, reference cell, reference tissue, control sample, control cell, or control tissue is a pooled RNA sample from normal tissue, or a pooled plasma or serum sample from one or more individuals that are not the patient. In certain cases, the reference sample, reference cell, reference tissue, control sample, control cell, or control tissue is a pooled RNA sample from synovial tissue, synovial fluid, or a pooled plasma or serum sample from one or more individuals that are not the patient and have a disease or disorder (e.g., RA).

[0166] B. Treatment method The present invention also provides a method for treating an individual with RA.Thus, in some cases, the method of the present invention comprises administering an RA therapeutic agent to an individual.Any of the RA therapeutic agents described herein or known in the art may be used in connection with this method.

[0167] As used herein, the term "genetic analysis" refers to the detection of one or more of the genes set forth in Table 1 in a sample from an individual (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, The present invention provides a method for treating an individual with RA, comprising: determining the expression level of one or more of the genes listed in Table 1 (43, 44, 45, or 46 genes), wherein the expression level of one or more of the genes listed in Table 1 is determined to be changed compared to a reference expression level; and (ii) administering an effective amount of an RA therapeutic agent (e.g., a therapeutic agent other than or in addition to a DMARD) to the individual based on the expression level of the one or more genes determined in step (i). In some cases, the change is an increase. In other cases, the change is a decrease.

[0168] As used herein, methods include (i) determining the expression level of one or more of the genes set forth in Table 2 (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, or 40 genes set forth in Table 2) in a sample from an individual, wherein the method further comprises: The present invention provides a method for treating an individual having RA, comprising: (i) determining that the expression level of one or more of the genes is increased compared to a reference expression level (e.g., an increased expression level of about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or more); and (ii) administering to the individual an effective amount of an RA therapeutic agent (e.g., a therapeutic agent other than or in addition to a DMARD) based on the expression level of the one or more genes determined in step (i). In certain cases, there is an increased expression of one or more genes listed in Table 2 (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, or 40 genes listed in Table 2). The expression level is an increase of at least about 1.1x, 1.2x, 1.3x, 1.4x, 1.5x, 1.6x, 1.7x, 1.8x, 1.9x, 2x, 2.1x, 2.2x, 2.3x, 2.4x, 2.5x, 2.6x, 2.7x, 2.8x, 2.9x, 3x, 3.5x, 4x, 4.5x, 5x, 6x, 7x, 8x, 9x, 10x, 15x, 20x, 30x, 40x, 50x, 100x, 500x, or 1000x as compared to a reference expression level of the one or more genes.In some cases, an increased expression level of one or more genes listed in Table 2 (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, or 40 genes listed in Table 2) may be a reference for the one or more genes. The increase is at least about 1.1 fold, about 1.2 fold, about 1.3 fold, about 1.4 fold, about 1.5 fold, about 1.6 fold, about 1.7 fold, about 1.8 fold, about 1.9 fold, about 2 fold, about 2.1 fold, about 2.2 fold, about 2.3 fold, about 2.4 fold, about 2.5 fold, about 2.6 fold, about 2.7 fold, about 2.8 fold, about 2.9 fold, about 3 fold, about 3.5 fold, about 4 fold, about 4.5 fold, about 5 fold, about 6 fold, about 7 fold, about 8 fold, about 9 fold, about 10 fold, about 15 fold, about 20 fold, about 30 fold, about 40 fold, about 50 fold, about 100 fold, about 500 fold, or about 1,000 fold or more as compared to the control expression level.

[0169] Provided herein is a method of treating an individual with RA, comprising: (i) determining an expression level of one or more of the genes set forth in Table 3 (e.g., one, two, three, four, five, or six genes set forth in Table 3) in a sample from the individual, wherein the expression level of one or more of the genes set forth in Table 3 is determined to be decreased compared to a reference expression level (e.g., a decreased expression level of about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or more); and (ii) administering to the individual an effective amount of an RA therapeutic agent (e.g., a therapeutic agent other than or in addition to a DMARD) based on the expression level of the one or more genes determined in step (i). In certain cases, the decreased expression level of one or more genes listed in Table 3 (e.g., 1, 2, 3, 4, 5, or 6 genes listed in Table 3) is at least about a 1.1x, 1.2x, 1.3x, 1.4x, 1.5x, 1.6x, 1.7x, 1.8x, 1.9x, 2x, 2.1x, 2.2x, 2.3x, 2.4x, 2.5x, 2.6x, 2.7x, 2.8x, 2.9x, 3x, 3.5x, 4x, 4.5x, 5x, 6x, 7x, 8x, 9x, 10x, 15x, 20x, 30x, 40x, 50x, 100x, 500x, or 1000x decrease compared to the reference expression level of the one or more genes. In some cases, the decreased expression level of one or more genes listed in Table 3 (e.g., 1, 2, 3, 4, 5, or 6 genes listed in Table 3) is at least about 1.1-fold, about 1.2-fold, about 1.3-fold, about 1.4-fold, about 1.5-fold, about 1.6-fold, about 1.7-fold, about 1.8-fold, about 1.9-fold, or about 1.8-fold relative to the reference expression level of the one or more genes. , about 2-fold, about 2.1-fold, about 2.2-fold, about 2.3-fold, about 2.4-fold, about 2.5-fold, about 2.6-fold, about 2.7-fold, about 2.8-fold, about 2.9-fold, about 3-fold, about 3.5-fold, about 4-fold, about 4.5-fold, about 5-fold, about 6-fold, about 7-fold, about 8-fold, about 9-fold, about 10-fold, about 15-fold, about 20-fold, about 30-fold, about 40-fold, about 50-fold, about 100-fold, about 500-fold, or about 1,000-fold or more.

[0170] Also provided is a method of treating an individual with RA, comprising administering an effective amount of an RA therapeutic agent (e.g., a therapeutic agent other than or in addition to a DMARD) to the individual, the individual having been identified as likely to exhibit disease progression by one or more of the predictive diagnostic methods described in Section II-A above.

[0171] The invention also provides a method for detecting a gene expression level of one or more genes set forth in Table 2 (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119 , 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, or 40 genes) have increased expression levels (e.g., about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95% , 96%, 97%, 98%, or 99% or more increased expression level), and / or (b) a decreased level (e.g., about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or more decreased expression level) of one or more genes set forth in Table 3 (e.g., 1, 2, 3, 4, 5, or 6 genes set forth in Table 3) in the sample; (iii) identifying the individual as having an increased likelihood that a therapeutic agent other than or in addition to a DMARD would be beneficial; and (iv) administering a therapeutic agent other than or in addition to a DMARD to the individual.In some cases, an increased expression level of one or more genes listed in Table 2 (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, or 40 genes listed in Table 2) may be a reference for the one or more genes. The increase is at least about 1.1 fold, about 1.2 fold, about 1.3 fold, about 1.4 fold, about 1.5 fold, about 1.6 fold, about 1.7 fold, about 1.8 fold, about 1.9 fold, about 2 fold, about 2.1 fold, about 2.2 fold, about 2.3 fold, about 2.4 fold, about 2.5 fold, about 2.6 fold, about 2.7 fold, about 2.8 fold, about 2.9 fold, about 3 fold, about 3.5 fold, about 4 fold, about 4.5 fold, about 5 fold, about 6 fold, about 7 fold, about 8 fold, about 9 fold, about 10 fold, about 15 fold, about 20 fold, about 30 fold, about 40 fold, about 50 fold, about 100 fold, about 500 fold, or about 1,000 fold or more as compared to the control expression level. In certain cases, the decreased expression level of one or more genes listed in Table 3 (e.g., 1, 2, 3, 4, 5, or 6 genes listed in Table 3) is at least about a 1.1x, 1.2x, 1.3x, 1.4x, 1.5x, 1.6x, 1.7x, 1.8x, 1.9x, 2x, 2.1x, 2.2x, 2.3x, 2.4x, 2.5x, 2.6x, 2.7x, 2.8x, 2.9x, 3x, 3.5x, 4x, 4.5x, 5x, 6x, 7x, 8x, 9x, 10x, 15x, 20x, 30x, 40x, 50x, 100x, 500x, or 1000x decrease compared to the reference expression level of the one or more genes.In some cases, the decreased expression level of one or more genes listed in Table 3 (e.g., 1, 2, 3, 4, 5, or 6 genes listed in Table 3) is at least about 1.1-fold, about 1.2-fold, about 1.3-fold, about 1.4-fold, about 1.5-fold, about 1.6-fold, about 1.7-fold, about 1.8-fold, about 1.9-fold, or about 1.8-fold relative to the reference expression level of the one or more genes. , about 2-fold, about 2.1-fold, about 2.2-fold, about 2.3-fold, about 2.4-fold, about 2.5-fold, about 2.6-fold, about 2.7-fold, about 2.8-fold, about 2.9-fold, about 3-fold, about 3.5-fold, about 4-fold, about 4.5-fold, about 5-fold, about 6-fold, about 7-fold, about 8-fold, about 9-fold, about 10-fold, about 15-fold, about 20-fold, about 30-fold, about 40-fold, about 50-fold, about 100-fold, about 500-fold, or about 1,000-fold or more.

[0172] The present invention also provides a method for the production of one or more genes set forth in Table 2 (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, or 40 genes set forth in Table 2). Also provided is a method of treating RA in an individual identified as having an increased expression level (e.g., about a 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or greater increased expression level) of one or more genes (e.g., a gene encoding a marker gene, a marker gene encoding a marker gene, or a gene encoding a marker gene) relative to a reference expression level of the one or more genes, the method comprising administering to the individual an effective amount of an RA therapeutic agent (e.g., a therapeutic agent other than or in addition to a DMARD). In certain cases, an increased expression level of one or more genes listed in Table 2 (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, or 40 genes listed in Table 2) compared to a reference expression level of the one or more genes. and the increase is at least about 1.1×, 1.2×, 1.3×, 1.4×, 1.5×, 1.6×, 1.7×, 1.8×, 1.9×, 2×, 2.1×, 2.2×, 2.3×, 2.4×, 2.5×, 2.6×, 2.7×, 2.8×, 2.9×, 3×, 3.5×, 4×, 4.5×, 5×, 6×, 7×, 8×, 9×, 10×, 15×, 20×, 30×, 40×, 50×, 100×, 500×, or 1000×, overall, and the method comprises administering to the individual an effective amount of an RA therapeutic agent (e.g., a therapeutic agent other than or in addition to a DMARD).In some cases, the increased expression level of one or more genes listed in Table 2 (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, or 40 genes listed in Table 2) is at least about 1.1-fold, about 1.2-fold, about 1.3-fold, about 1.4-fold, or about 1.5-fold, or about 1.6-fold, or about 1.7-fold, or about 1.8-fold, or about 1.9-fold, or about 20 ... an increase of about 1.3-fold, about 1.4-fold, about 1.5-fold, about 1.6-fold, about 1.7-fold, about 1.8-fold, about 1.9-fold, about 2-fold, about 2.1-fold, about 2.2-fold, about 2.3-fold, about 2.4-fold, about 2.5-fold, about 2.6-fold, about 2.7-fold, about 2.8-fold, about 2.9-fold, about 3-fold, about 3.5-fold, about 4-fold, about 4.5-fold, about 5-fold, about 6-fold, about 7-fold, about 8-fold, about 9-fold, about 10-fold, about 15-fold, about 20-fold, about 30-fold, about 40-fold, about 50-fold, about 100-fold, about 500-fold, or about 1,000-fold or more, and the method comprises administering to the individual an effective amount of an RA therapeutic agent (e.g., a therapeutic agent other than or in addition to a DMARD).

[0173] In another instance, the invention provides a method of treating RA in an individual who has been identified as having an expression level in a sample from the individual of one or more genes selected from CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A (e.g., 1, 2, 3, 4, 5, 6, or 7 genes selected from CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A) that is equal to or greater than a reference expression level of the one or more genes (e.g., greater than or about a 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or greater increase therein), the method comprising administering to the individual an RA therapeutic agent (e.g., a therapeutic agent other than or in addition to a DMARD). In certain cases, an increased expression level of one or more genes selected from CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A (e.g., 1, 2, 3, 4, 5, 6, or 7 genes selected from CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A) may be indicative of a reference expression level of the one or more genes. An increase of at least about 1.1×, 1.2×, 1.3×, 1.4×, 1.5×, 1.6×, 1.7×, 1.8×, 1.9×, 2×, 2.1×, 2.2×, 2.3×, 2.4×, 2.5×, 2.6×, 2.7×, 2.8×, 2.9×, 3×, 3.5×, 4×, 4.5×, 5×, 6×, 7×, 8×, 9×, 10×, 15×, 20×, 30×, 40×, 50×, 100×, 500×, or 1000× compared to the current level.In some cases, the increased expression level of one or more genes selected from CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A (e.g., 1, 2, 3, 4, 5, 6, or 7 genes selected from CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A) is at least about 1.1-fold, about 1.0-fold, or more than about 1.5 ... The increase is about 0.2-fold, about 1.3-fold, about 1.4-fold, about 1.5-fold, about 1.6-fold, about 1.7-fold, about 1.8-fold, about 1.9-fold, about 2-fold, about 2.1-fold, about 2.2-fold, about 2.3-fold, about 2.4-fold, about 2.5-fold, about 2.6-fold, about 2.7-fold, about 2.8-fold, about 2.9-fold, about 3-fold, about 3.5-fold, about 4-fold, about 4.5-fold, about 5-fold, about 6-fold, about 7-fold, about 8-fold, about 9-fold, about 10-fold, about 15-fold, about 20-fold, about 30-fold, about 40-fold, about 50-fold, about 100-fold, about 500-fold, or about 1,000-fold or more.

[0174] In another instance, the invention provides a method of treating RA in an individual who has been identified as having an expression level in a sample from the individual that is equal to or greater than a reference expression level (e.g., greater than or about a 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% increase therein) of at least two, at least three, at least four, at least five, at least six, or all seven of the following genes in a sample from the individual: CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, the method comprising administering to the individual an RA therapeutic agent (e.g., a therapeutic agent other than or in addition to a DMARD). In certain instances, the increased expression levels of at least two, at least three, at least four, at least five, at least six, or all seven genes selected from CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A are greater than or equal to the reference expression levels of at least two, at least three, at least four, at least five, at least six, or all seven genes. In some embodiments, the increase is at least about a 1.1×, 1.2×, 1.3×, 1.4×, 1.5×, 1.6×, 1.7×, 1.8×, 1.9×, 2×, 2.1×, 2.2×, 2.3×, 2.4×, 2.5×, 2.6×, 2.7×, 2.8×, 2.9×, 3×, 3.5×, 4×, 4.5×, 5×, 6×, 7×, 8×, 9×, 10×, 15×, 20×, 30×, 40×, 50×, 100×, 500×, or 1000× increase compared to the previous embodiment.In some instances, the increased expression level of at least two, at least three, at least four, at least five, at least six, or all seven genes selected from CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A is at least about 1.1-fold, about 1.2-fold, or about 1.3-fold, or about 1.4-fold, or about 1.5-fold, or about 1.6-fold, or about 1.7-fold, or about 1.8-fold, or about 1.9 ...9-fold, or about 1. fold, about 1.3 fold, about 1.4 fold, about 1.5 fold, about 1.6 fold, about 1.7 fold, about 1.8 fold, about 1.9 fold, about 2 fold, about 2.1 fold, about 2.2 fold, about 2.3 fold, about 2.4 fold, about 2.5 fold, about 2.6 fold, about 2.7 fold, about 2.8 fold, about 2.9 fold, about 3 fold, about 3.5 fold, about 4 fold, about 4.5 fold, about 5 fold, about 6 fold, about 7 fold, about 8 fold, about 9 fold, about 10 fold, about 15 fold, about 20 fold, about 30 fold, about 40 fold, about 50 fold, about 100 fold, about 500 fold, or about 1,000 fold or more increase.

[0175] In another example, the present invention provides a method of treating RA in an individual who has been identified as having an expression level in a sample from the individual of one or more genes (e.g., one, two, three, four, five, or six) set forth in Table 3 that is equal to or lower than a reference expression level for the one or more genes (e.g., below the expression level or by about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or more reduction therebetween), the method comprising administering to the individual an RA therapeutic agent (e.g., a therapeutic agent other than or in addition to a DMARD). In certain cases, the decreased expression level of one or more genes listed in Table 3 (e.g., 1, 2, 3, 4, 5, or 6 genes listed in Table 3) is at least about a 1.1x, 1.2x, 1.3x, 1.4x, 1.5x, 1.6x, 1.7x, 1.8x, 1.9x, 2x, 2.1x, 2.2x, 2.3x, 2.4x, 2.5x, 2.6x, 2.7x, 2.8x, 2.9x, 3x, 3.5x, 4x, 4.5x, 5x, 6x, 7x, 8x, 9x, 10x, 15x, 20x, 30x, 40x, 50x, 100x, 500x, or 1000x decrease compared to the reference expression level of the one or more genes. In some cases, the decreased expression level of one or more genes listed in Table 3 (e.g., 1, 2, 3, 4, 5, or 6 genes listed in Table 3) is at least about 1.1-fold, about 1.2-fold, about 1.3-fold, about 1.4-fold, about 1.5-fold, about 1.6-fold, about 1.7-fold, about 1.8-fold, about 1.9-fold, or about 1.8-fold relative to the reference expression level of the one or more genes. , about 2-fold, about 2.1-fold, about 2.2-fold, about 2.3-fold, about 2.4-fold, about 2.5-fold, about 2.6-fold, about 2.7-fold, about 2.8-fold, about 2.9-fold, about 3-fold, about 3.5-fold, about 4-fold, about 4.5-fold, about 5-fold, about 6-fold, about 7-fold, about 8-fold, about 9-fold, about 10-fold, about 15-fold, about 20-fold, about 30-fold, about 40-fold, about 50-fold, about 100-fold, about 500-fold, or about 1,000-fold or more.

[0176] In any of the above-mentioned treatment methods, the method may further include determining one or more clinical covariates of the individual (e.g., baseline RF titer, disease duration, DAS28-ESR, DAS28-CRP, baseline pathology, and USST and USPD 12 max scores).

[0177] In any of the above-mentioned treatment methods, the reference expression level can be a reference expression level in a reference population of individuals with RA who have not been previously treated with DMARDs, the population of individuals being composed of a first subset of individuals who have disease progression and a second subset of individuals who have not disease progression.In some cases, the reference expression level significantly separates each of the first and second subsets of individuals based on the significant difference between the expression level of one or more genes listed in Table 1, Table 2 or Table 3 in the first subset of individuals compared with that of the second subset of individuals.In some cases, after about 12 months, the first subset of individuals has disease progression and the second subset of individuals has not disease progression.

[0178] In any of the above-mentioned treatment methods in which the expression levels of more than one biomarker selected from the genes listed in Table 1, Table 2, or Table 3 are determined in a sample from an individual and compared to a reference expression level (e.g., a pre-assigned expression level of one or more genes listed in Table 1, Table 2, or Table 3), it should be understood that in some cases, the expression level of each individual biomarker in the sample is compared to the reference expression level of each individual biomarker. For example, when the expression levels of CD180 and CXCL1 are determined in a sample from an individual and compared to the reference expression levels of CD180 and CXCL1, in some cases, the expression level of CD180 in the sample from the individual is compared to the reference expression level of CD180, and the expression level of CXCL1 in the sample from the individual is compared to the reference expression level of CXCL1. In other cases, the expression levels of more than one gene of interest may be determined by counting methods known to those of skill in the art and also disclosed herein, including, for example, calculating the median or mean of all the expression levels of the genes of interest. Prior to counting, the expression level of each gene of interest may be normalized by using statistical methods known to those skilled in the art and disclosed herein, including, for example, normalizing to the expression level of one or more housekeeping genes, or normalizing to the total library size, or normalizing to the median or average expression level across all genes measured. In some cases, prior to counting across multiple genes of interest, the normalized expression level of each gene of interest may be standardized by using statistical methods known to those skilled in the art and disclosed herein, including, for example, calculating the Z-score of the normalized expression level of each gene of interest.

[0179] In any of the above-mentioned treatment methods, in which the expression level of one or more biomarkers selected from the biomarkers listed in Table 1, Table 2, or Table 3 is determined in a sample from an individual and compared to a reference expression level (e.g., a pre-assigned expression level of one or more genes listed in Table 1, Table 2, or Table 3), it is understood that in some cases, the expression level of one or more genes may be the average value of the expression level of one or more genes. In some cases, the expression level of one or more genes may be the median value of the expression level of one or more genes. In some cases, the expression level of one or more genes may be normalized to, for example, a reference gene, e.g., a housekeeping gene. In some cases, the reference gene is ACTB, GAPDH, GUSB, HPRT1, PGK1, RPL19, TUBB, or TMEM55B. In some cases, the expression level of one or more genes may be the average value of the normalized expression level of one or more genes. In some cases, the expression level of one or more genes may be the median value of the normalized expression level of one or more genes.

[0180] The invention provides methods that can be used to treat an individual having RA with an RA therapeutic (e.g., an RA therapeutic comprising a DMARD such as methotrexate) involving: (i) obtaining a sample from an individual; (ii) performing a gene expression assay on the sample to determine a myeloid intrinsic gene score that is equal to or increased compared to a reference myeloid intrinsic gene score (e.g., an increased myeloid intrinsic gene score of about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or more); (iii) identifying the individual as having an increased likelihood of benefiting from an RA therapeutic (e.g., an RA therapeutic comprising a DMARD such as methotrexate); and (iv) administering the RA therapeutic (e.g., an RA therapeutic comprising a DMARD such as methotrexate) to the individual. In some cases, an increased myeloid-specific gene score refers to an overall increase of at least about 1.1×, 1.2×, 1.3×, 1.4×, 1.5×, 1.6×, 1.7×, 1.8×, 1.9×, 2×, 2.1×, 2.2×, 2.3×, 2.4×, 2.5×, 2.6×, 2.7×, 2.8×, 2.9×, 3×, 3.5×, 4×, 4.5×, 5×, 6×, 7×, 8×, 9×, 10×, 15×, 20×, 30×, 40×, 50×, 100×, 500×, or 1000× as compared to a reference myeloid-specific gene score. In some cases, an increased myeloid-specific gene score refers to an overall increase in the myeloid-specific gene score of more than about 1.1 fold, about 1.2 fold, about 1.3 fold, about 1.4 fold, about 1.5 fold, about 1.6 fold, about 1.7 fold, about 1.8 fold, about 1.9 fold, about 2 fold, about 2.1 fold, about 2.2 fold, about 2.3 fold, about 2.4 fold, about 2.5 fold, about 2.6 fold, about 2.7 fold, about 2.8 fold, about 2.9 fold, about 3 fold, about 3.5 fold, about 4 fold, about 4.5 fold, about 5 fold, about 6 fold, about 7 fold, about 8 fold, about 9 fold, about 10 fold, about 15 fold, about 20 fold, about 30 fold, about 40 fold, about 50 fold, about 100 fold, about 500 fold, about 1,000 fold or more as compared to a reference myeloid-specific gene score.In some cases, the method may further include performing a gene expression assay on the sample to determine that the lymphoid-specific gene score is equivalent to or increased compared to a reference lymphoid-specific gene score (e.g., an increased lymphoid-specific gene score of about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or more) prior to identifying the individual as having an increased likelihood of benefiting from an RA therapeutic (e.g., an RA therapeutic including a DMARD such as methotrexate). In some cases, an increased lymphoid-specific gene score refers to an overall increase of at least about 1.1×, 1.2×, 1.3×, 1.4×, 1.5×, 1.6×, 1.7×, 1.8×, 1.9×, 2×, 2.1×, 2.2×, 2.3×, 2.4×, 2.5×, 2.6×, 2.7×, 2.8×, 2.9×, 3×, 3.5×, 4×, 4.5×, 5×, 6×, 7×, 8×, 9×, 10×, 15×, 20×, 30×, 40×, 50×, 100×, 500×, or 1000× as compared to a reference lymphoid-specific gene score. In some cases, an increased lymphoid-specific gene score refers to an overall increase in the lymphoid-specific gene score of more than about 1.1 fold, about 1.2 fold, about 1.3 fold, about 1.4 fold, about 1.5 fold, about 1.6 fold, about 1.7 fold, about 1.8 fold, about 1.9 fold, about 2 fold, about 2.1 fold, about 2.2 fold, about 2.3 fold, about 2.4 fold, about 2.5 fold, about 2.6 fold, about 2.7 fold, about 2.8 fold, about 2.9 fold, about 3 fold, about 3.5 fold, about 4 fold, about 4.5 fold, about 5 fold, about 6 fold, about 7 fold, about 8 fold, about 9 fold, about 10 fold, about 15 fold, about 20 fold, about 30 fold, about 40 fold, about 50 fold, about 100 fold, about 500 fold, about 1,000 fold or more as compared to a reference lymphoid-specific gene score.

[0181] The present invention provides a method that can be used to treat an individual having RA with an RA therapy (e.g., an RA therapy including a DMARD such as methotrexate) involving: (i) determining a myeloid intrinsic gene score from a sample from an individual, wherein the myeloid intrinsic gene score from the sample is determined to be equal to or greater than a reference myeloid intrinsic gene score (e.g., an increase of about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or more above the myeloid intrinsic gene score); and (ii) administering an effective amount of an RA therapy (e.g., an RA therapy including a DMARD such as methotrexate) to the individual based on the myeloid intrinsic gene score of step (i). In some cases, an elevated or increased myeloid-specific gene score refers to an overall increase of at least about 1.1×, 1.2×, 1.3×, 1.4×, 1.5×, 1.6×, 1.7×, 1.8×, 1.9×, 2×, 2.1×, 2.2×, 2.3×, 2.4×, 2.5×, 2.6×, 2.7×, 2.8×, 2.9×, 3×, 3.5×, 4×, 4.5×, 5×, 6×, 7×, 8×, 9×, 10×, 15×, 20×, 30×, 40×, 50×, 100×, 500×, or 1000× as compared to a reference myeloid-specific gene score. In some cases, an elevated myeloid-specific gene score refers to an overall increase in the myeloid-specific gene score of more than about 1.1 fold, about 1.2 fold, about 1.3 fold, about 1.4 fold, about 1.5 fold, about 1.6 fold, about 1.7 fold, about 1.8 fold, about 1.9 fold, about 2 fold, about 2.1 fold, about 2.2 fold, about 2.3 fold, about 2.4 fold, about 2.5 fold, about 2.6 fold, about 2.7 fold, about 2.8 fold, about 2.9 fold, about 3 fold, about 3.5 fold, about 4 fold, about 4.5 fold, about 5 fold, about 6 fold, about 7 fold, about 8 fold, about 9 fold, about 10 fold, about 15 fold, about 20 fold, about 30 fold, about 40 fold, about 50 fold, about 100 fold, about 500 fold, about 1,000 fold or more as compared to a reference myeloid-specific gene score.In some cases, the method may further involve determining a lymphoid-specific gene score from a sample from the individual prior to administration of an RA therapy (e.g., an RA therapy including a DMARD such as methotrexate), where the lymphoid-specific gene score from the sample is determined to be equal to or greater than a reference lymphoid-specific gene score (e.g., greater than or about a 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or greater increase above the lymphoid-specific gene score). In some cases, an elevated or increased lymphoid-specific gene score refers to an overall increase of at least about 1.1×, 1.2×, 1.3×, 1.4×, 1.5×, 1.6×, 1.7×, 1.8×, 1.9×, 2×, 2.1×, 2.2×, 2.3×, 2.4×, 2.5×, 2.6×, 2.7×, 2.8×, 2.9×, 3×, 3.5×, 4×, 4.5×, 5×, 6×, 7×, 8×, 9×, 10×, 15×, 20×, 30×, 40×, 50×, 100×, 500×, or 1000× as compared to a reference lymphoid-specific gene score. In some cases, an elevated lymphoid-specific gene score refers to an overall increase in the lymphoid-specific gene score of more than about 1.1 fold, about 1.2 fold, about 1.3 fold, about 1.4 fold, about 1.5 fold, about 1.6 fold, about 1.7 fold, about 1.8 fold, about 1.9 fold, about 2 fold, about 2.1 fold, about 2.2 fold, about 2.3 fold, about 2.4 fold, about 2.5 fold, about 2.6 fold, about 2.7 fold, about 2.8 fold, about 2.9 fold, about 3 fold, about 3.5 fold, about 4 fold, about 4.5 fold, about 5 fold, about 6 fold, about 7 fold, about 8 fold, about 9 fold, about 10 fold, about 15 fold, about 20 fold, about 30 fold, about 40 fold, about 50 fold, about 100 fold, about 500 fold, about 1,000 fold or more as compared to a reference lymphoid-specific gene score.

[0182] Also provided is a method of treating an individual with RA, comprising administering an effective amount of an RA therapeutic (e.g., an RA therapeutic comprising a DMARD such as methotrexate) to the individual, the individual having been identified as likely to benefit from an RA therapeutic (e.g., an RA therapeutic comprising a DMARD such as methotrexate) by one or more of the predictive diagnostic methods described in Section II-A above. Thus, in some cases, the individual has been identified as likely to benefit from an RA treatment (e.g., an RA therapeutic comprising a DMARD such as methotrexate) based on a myeloid intrinsic gene score from a sample from the individual that is determined to be equal to or greater than a reference myeloid intrinsic gene score (e.g., greater than or equal to about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or more increase therebetween). In some cases, an elevated or increased myeloid-specific gene score refers to an overall increase of at least about 1.1×, 1.2×, 1.3×, 1.4×, 1.5×, 1.6×, 1.7×, 1.8×, 1.9×, 2×, 2.1×, 2.2×, 2.3×, 2.4×, 2.5×, 2.6×, 2.7×, 2.8×, 2.9×, 3×, 3.5×, 4×, 4.5×, 5×, 6×, 7×, 8×, 9×, 10×, 15×, 20×, 30×, 40×, 50×, 100×, 500×, or 1000× as compared to a reference myeloid-specific gene score. In some cases, an elevated myeloid-specific gene score refers to an overall increase in the myeloid-specific gene score of more than about 1.1 fold, about 1.2 fold, about 1.3 fold, about 1.4 fold, about 1.5 fold, about 1.6 fold, about 1.7 fold, about 1.8 fold, about 1.9 fold, about 2 fold, about 2.1 fold, about 2.2 fold, about 2.3 fold, about 2.4 fold, about 2.5 fold, about 2.6 fold, about 2.7 fold, about 2.8 fold, about 2.9 fold, about 3 fold, about 3.5 fold, about 4 fold, about 4.5 fold, about 5 fold, about 6 fold, about 7 fold, about 8 fold, about 9 fold, about 10 fold, about 15 fold, about 20 fold, about 30 fold, about 40 fold, about 50 fold, about 100 fold, about 500 fold, about 1,000 fold or more as compared to a reference myeloid-specific gene score.In some cases, prior to administration of an RA therapy (e.g., an RA therapy including a DMARD such as methotrexate) to the individual, the individual is identified as one who is likely to benefit from an RA therapy (e.g., an RA therapy including a DMARD such as methotrexate) further based on a lymphoid intrinsic gene score from a sample from the individual that has been determined to be equal to or greater than a reference lymphoid intrinsic gene score (e.g., an increase of about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or more above the lymphoid intrinsic gene score). In some cases, an elevated or increased lymphoid-specific gene score refers to an overall increase of at least about 1.1×, 1.2×, 1.3×, 1.4×, 1.5×, 1.6×, 1.7×, 1.8×, 1.9×, 2×, 2.1×, 2.2×, 2.3×, 2.4×, 2.5×, 2.6×, 2.7×, 2.8×, 2.9×, 3×, 3.5×, 4×, 4.5×, 5×, 6×, 7×, 8×, 9×, 10×, 15×, 20×, 30×, 40×, 50×, 100×, 500×, or 1000× as compared to a reference lymphoid-specific gene score. In some cases, an elevated lymphoid-specific gene score refers to an overall increase in the lymphoid-specific gene score of more than about 1.1 fold, about 1.2 fold, about 1.3 fold, about 1.4 fold, about 1.5 fold, about 1.6 fold, about 1.7 fold, about 1.8 fold, about 1.9 fold, about 2 fold, about 2.1 fold, about 2.2 fold, about 2.3 fold, about 2.4 fold, about 2.5 fold, about 2.6 fold, about 2.7 fold, about 2.8 fold, about 2.9 fold, about 3 fold, about 3.5 fold, about 4 fold, about 4.5 fold, about 5 fold, about 6 fold, about 7 fold, about 8 fold, about 9 fold, about 10 fold, about 15 fold, about 20 fold, about 30 fold, about 40 fold, about 50 fold, about 100 fold, about 500 fold, about 1,000 fold or more as compared to a reference lymphoid-specific gene score.

[0183] Also provided is a method of treating an individual with RA, comprising administering an effective amount of an RA therapeutic (e.g., an RA therapeutic comprising a DMARD such as methotrexate) to the individual, the individual being identified as having a myeloid intrinsic gene score from a sample from the individual that is equal to or greater than a reference myeloid intrinsic gene score by one or more of the predictive diagnostic methods described in Section II-A above.Thus, in some cases, the individual being identified as having a myeloid intrinsic gene score from a sample from the individual that is equal to or greater than a reference myeloid intrinsic gene score (e.g., greater than or about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or more increase therebetween). In some cases, an elevated or increased myeloid-specific gene score refers to an overall increase of at least about 1.1×, 1.2×, 1.3×, 1.4×, 1.5×, 1.6×, 1.7×, 1.8×, 1.9×, 2×, 2.1×, 2.2×, 2.3×, 2.4×, 2.5×, 2.6×, 2.7×, 2.8×, 2.9×, 3×, 3.5×, 4×, 4.5×, 5×, 6×, 7×, 8×, 9×, 10×, 15×, 20×, 30×, 40×, 50×, 100×, 500×, or 1000× as compared to a reference myeloid-specific gene score. In some cases, an elevated myeloid-specific gene score refers to an overall increase in the myeloid-specific gene score of more than about 1.1 fold, about 1.2 fold, about 1.3 fold, about 1.4 fold, about 1.5 fold, about 1.6 fold, about 1.7 fold, about 1.8 fold, about 1.9 fold, about 2 fold, about 2.1 fold, about 2.2 fold, about 2.3 fold, about 2.4 fold, about 2.5 fold, about 2.6 fold, about 2.7 fold, about 2.8 fold, about 2.9 fold, about 3 fold, about 3.5 fold, about 4 fold, about 4.5 fold, about 5 fold, about 6 fold, about 7 fold, about 8 fold, about 9 fold, about 10 fold, about 15 fold, about 20 fold, about 30 fold, about 40 fold, about 50 fold, about 100 fold, about 500 fold, about 1,000 fold or more as compared to a reference myeloid-specific gene score.In some cases, prior to administration of an RA therapy (e.g., an RA therapy including a DMARD such as methotrexate) to an individual, the individual is identified as having a lymphoid-specific gene score from a sample derived from the individual that is equal to or greater than a reference lymphoid-specific gene score (e.g., greater than or equal to about a 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% increase therebetween). In some cases, an elevated or increased lymphoid-specific gene score refers to an overall increase of at least about 1.1×, 1.2×, 1.3×, 1.4×, 1.5×, 1.6×, 1.7×, 1.8×, 1.9×, 2×, 2.1×, 2.2×, 2.3×, 2.4×, 2.5×, 2.6×, 2.7×, 2.8×, 2.9×, 3×, 3.5×, 4×, 4.5×, 5×, 6×, 7×, 8×, 9×, 10×, 15×, 20×, 30×, 40×, 50×, 100×, 500×, or 1000× as compared to a reference lymphoid-specific gene score. In some cases, an elevated lymphoid-specific gene score refers to an overall increase in the lymphoid-specific gene score of more than about 1.1 fold, about 1.2 fold, about 1.3 fold, about 1.4 fold, about 1.5 fold, about 1.6 fold, about 1.7 fold, about 1.8 fold, about 1.9 fold, about 2 fold, about 2.1 fold, about 2.2 fold, about 2.3 fold, about 2.4 fold, about 2.5 fold, about 2.6 fold, about 2.7 fold, about 2.8 fold, about 2.9 fold, about 3 fold, about 3.5 fold, about 4 fold, about 4.5 fold, about 5 fold, about 6 fold, about 7 fold, about 8 fold, about 9 fold, about 10 fold, about 15 fold, about 20 fold, about 30 fold, about 40 fold, about 50 fold, about 100 fold, about 500 fold, about 1,000 fold or more as compared to a reference lymphoid-specific gene score.

[0184] In certain cases, the methods provided herein may be used to optimize the therapeutic efficacy of RA therapeutics (e.g., RA therapeutics including DMARDs such as methotrexate), and the methods include monitoring myeloid intrinsic gene scores from samples from an individual during (e.g., over the treatment period) treatment with RA therapeutics. Monitoring may include, for example, obtaining and comparing myeloid intrinsic gene scores from samples from an individual collected at time intervals before and / or after administration of RA therapeutics (e.g., RA therapeutics including DMARDs such as methotrexate). In some cases, the myeloid intrinsic gene score may be obtained from a sample from an individual collected at least about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, or 24 hours, about 1, 2, 3, 4, 5, 6, 7 days, about 1, 2, 3, or 4 weeks, or about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 months prior to administration of an RA therapy (e.g., an RA therapy including a DMARD such as methotrexate). In some cases, the myeloid intrinsic gene score may be obtained from a sample from the individual collected at least about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, or 24 hours, about 1, 2, 3, 4, 5, 6, 7 days, about 1, 2, 3, or 4 weeks, or about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 months after administration of an RA therapy (e.g., an RA therapy including a DMARD such as methotrexate).Myeloid intrinsic gene scores from samples from an individual collected before and / or after administration of an RA therapy (e.g., an RA therapy including a DMARD such as methotrexate) may be compared, and an increase in the myeloid intrinsic gene score from a sample from an individual collected after treatment compared to a sample collected before treatment (e.g., an increase of about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or more in the myeloid intrinsic gene score) may be observed. ) may indicate a low level of therapeutic efficacy of the administered RA therapeutic, and a decrease in myeloid intrinsic gene score from a sample from an individual collected after treatment compared to a myeloid intrinsic gene score from a sample collected before treatment (e.g., about a 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or greater decrease in myeloid intrinsic gene score) may indicate therapeutic efficacy of the administered RA therapeutic (e.g., an RA therapeutic including a DMARD such as methotrexate). In some cases, an elevated or increased myeloid-specific gene score refers to an overall increase of at least about 1.1×, 1.2×, 1.3×, 1.4×, 1.5×, 1.6×, 1.7×, 1.8×, 1.9×, 2×, 2.1×, 2.2×, 2.3×, 2.4×, 2.5×, 2.6×, 2.7×, 2.8×, 2.9×, 3×, 3.5×, 4×, 4.5×, 5×, 6×, 7×, 8×, 9×, 10×, 15×, 20×, 30×, 40×, 50×, 100×, 500×, or 1000× as compared to a reference myeloid-specific gene score. In some cases, an elevated myeloid-specific gene score refers to an overall increase in the myeloid-specific gene score of more than about 1.1 fold, about 1.2 fold, about 1.3 fold, about 1.4 fold, about 1.5 fold, about 1.6 fold, about 1.7 fold, about 1.8 fold, about 1.9 fold, about 2 fold, about 2.1 fold, about 2.2 fold, about 2.3 fold, about 2.4 fold, about 2.5 fold, about 2.6 fold, about 2.7 fold, about 2.8 fold, about 2.9 fold, about 3 fold, about 3.5 fold, about 4 fold, about 4.5 fold, about 5 fold, about 6 fold, about 7 fold, about 8 fold, about 9 fold, about 10 fold, about 15 fold, about 20 fold, about 30 fold, about 40 fold, about 50 fold, about 100 fold, about 500 fold, about 1,000 fold or more as compared to a reference myeloid-specific gene score.In some cases, a reduced or decreased myeloid intrinsic gene score refers to an overall decrease of at least about 1.1×, 1.2×, 1.3×, 1.4×, 1.5×, 1.6×, 1.7×, 1.8×, 1.9×, 2×, 2.1×, 2.2×, 2.3×, 2.4×, 2.5×, 2.6×, 2.7×, 2.8×, 2.9×, 3×, 3.5×, 4×, 4.5×, 5×, 6×, 7×, 8×, 9×, 10×, 15×, 20×, 30×, 40×, 50×, 100×, 500×, or 1000× as compared to a reference myeloid intrinsic gene score. In some cases, a reduced myeloid intrinsic gene score refers to an overall decrease in the myeloid intrinsic gene score of more than about 1.1 fold, about 1.2 fold, about 1.3 fold, about 1.4 fold, about 1.5 fold, about 1.6 fold, about 1.7 fold, about 1.8 fold, about 1.9 fold, about 2 fold, about 2.1 fold, about 2.2 fold, about 2.3 fold, about 2.4 fold, about 2.5 fold, about 2.6 fold, about 2.7 fold, about 2.8 fold, about 2.9 fold, about 3 fold, about 3.5 fold, about 4 fold, about 4.5 fold, about 5 fold, about 6 fold, about 7 fold, about 8 fold, about 9 fold, about 10 fold, about 15 fold, about 20 fold, about 30 fold, about 40 fold, about 50 fold, about 100 fold, about 500 fold, about 1,000 fold or more as compared to a reference myeloid intrinsic gene score.

[0185] In some cases, the reference myeloid intrinsic gene score may be obtained from the individual prior to treatment with an RA therapy (e.g., an RA therapy including a DMARD such as methotrexate). In some cases, the method includes monitoring the myeloid intrinsic gene score from a sample from the individual during (e.g., over the treatment period) treatment with an RA therapy (e.g., an RA therapy including a DMARD such as methotrexate) compared to the pre-treatment myeloid intrinsic gene score.

[0186] In certain cases, the methods provided herein may further include monitoring the lymphoid intrinsic gene score from a sample from the individual during (e.g., over the treatment period) treatment with an RA therapeutic (e.g., an RA therapeutic including a DMARD such as methotrexate). Monitoring may include, for example, obtaining and comparing lymphoid intrinsic gene scores from samples from the individual collected at time intervals before and / or after administration of an RA therapeutic (e.g., an RA therapeutic including a DMARD such as methotrexate). In some cases, the lymphoid specific gene score may be obtained from a sample from an individual collected at least about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, or 24 hours, about 1, 2, 3, 4, 5, 6, 7 days, about 1, 2, 3, or 4 weeks, or about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 months prior to administration of an RA therapy (e.g., an RA therapy including a DMARD such as methotrexate). In some cases, the lymphoid specific gene score may be obtained from a sample from the individual collected at least about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, or 24 hours, about 1, 2, 3, 4, 5, 6, 7 days, about 1, 2, 3, or 4 weeks, or about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 months after administration of an RA therapy (e.g., an RA therapy including a DMARD such as methotrexate).The lymphoid-specific gene scores from samples from the individual collected before and / or after administration of an RA therapy (e.g., an RA therapy including a DMARD such as methotrexate) may be compared, and an increase in the lymphoid-specific gene score from a sample from the individual collected after treatment compared to the lymphoid-specific gene score from a sample collected before treatment (e.g., an increase of about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or more in the lymphoid-specific gene score) is indicative of an increase in the lymphoid-specific gene score from the sample collected after treatment compared to the lymphoid-specific gene score from a sample collected before treatment. For example, a low level of therapeutic efficacy of an RA therapeutic agent (including a DMARD such as methotrexate) may be indicated, and a decrease in lymphoid-specific gene score from a sample collected from an individual after treatment compared to a sample collected before treatment (e.g., a decrease of about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or more in lymphoid-specific gene score) may indicate therapeutic efficacy of an administered RA therapeutic agent (e.g., an RA therapeutic agent including a DMARD such as methotrexate). In some cases, a reference lymphoid-specific gene score may be obtained from an individual before treatment with an RA therapeutic agent (e.g., an RA therapeutic agent including a DMARD such as methotrexate). In some cases, the method includes monitoring the lymphoid intrinsic gene score from a sample from the individual during (e.g., over a treatment period) treatment with an RA therapeutic (e.g., an RA therapeutic including a DMARD such as methotrexate) compared to the lymphoid intrinsic gene score before treatment. In some cases, an elevated or increased lymphoid-specific gene score refers to an overall increase of at least about 1.1×, 1.2×, 1.3×, 1.4×, 1.5×, 1.6×, 1.7×, 1.8×, 1.9×, 2×, 2.1×, 2.2×, 2.3×, 2.4×, 2.5×, 2.6×, 2.7×, 2.8×, 2.9×, 3×, 3.5×, 4×, 4.5×, 5×, 6×, 7×, 8×, 9×, 10×, 15×, 20×, 30×, 40×, 50×, 100×, 500×, or 1000× as compared to a reference lymphoid-specific gene score.In some cases, an elevated lymphoid-specific gene score refers to an overall increase in the lymphoid-specific gene score of more than about 1.1 fold, about 1.2 fold, about 1.3 fold, about 1.4 fold, about 1.5 fold, about 1.6 fold, about 1.7 fold, about 1.8 fold, about 1.9 fold, about 2 fold, about 2.1 fold, about 2.2 fold, about 2.3 fold, about 2.4 fold, about 2.5 fold, about 2.6 fold, about 2.7 fold, about 2.8 fold, about 2.9 fold, about 3 fold, about 3.5 fold, about 4 fold, about 4.5 fold, about 5 fold, about 6 fold, about 7 fold, about 8 fold, about 9 fold, about 10 fold, about 15 fold, about 20 fold, about 30 fold, about 40 fold, about 50 fold, about 100 fold, about 500 fold, about 1,000 fold or more as compared to a reference lymphoid-specific gene score. In some cases, a reduced or decreased lymphoid-specific gene score refers to an overall decrease of at least about 1.1×, 1.2×, 1.3×, 1.4×, 1.5×, 1.6×, 1.7×, 1.8×, 1.9×, 2×, 2.1×, 2.2×, 2.3×, 2.4×, 2.5×, 2.6×, 2.7×, 2.8×, 2.9×, 3×, 3.5×, 4×, 4.5×, 5×, 6×, 7×, 8×, 9×, 10×, 15×, 20×, 30×, 40×, 50×, 100×, 500×, or 1000× as compared to a reference lymphoid-specific gene score. In some cases, a reduced lymphoid-specific gene score refers to an overall decrease in the lymphoid-specific gene score of more than about 1.1 fold, about 1.2 fold, about 1.3 fold, about 1.4 fold, about 1.5 fold, about 1.6 fold, about 1.7 fold, about 1.8 fold, about 1.9 fold, about 2 fold, about 2.1 fold, about 2.2 fold, about 2.3 fold, about 2.4 fold, about 2.5 fold, about 2.6 fold, about 2.7 fold, about 2.8 fold, about 2.9 fold, about 3 fold, about 3.5 fold, about 4 fold, about 4.5 fold, about 5 fold, about 6 fold, about 7 fold, about 8 fold, about 9 fold, about 10 fold, about 15 fold, about 20 fold, about 30 fold, about 40 fold, about 50 fold, about 100 fold, about 500 fold, about 1,000 fold or more as compared to a reference lymphoid-specific gene score.

[0187] In certain cases, the method may include: (i) determining a first myeloid intrinsic gene score from a sample from the individual at a first time point during or after administration of a DMARD; (ii) determining a second myeloid intrinsic gene score from a sample from the individual at a second time point; and (iii) comparing the first myeloid intrinsic gene score to a second myeloid intrinsic gene score, and a decrease in the second myeloid intrinsic gene score compared to the first myeloid intrinsic gene score below a reference intrinsic gene score (e.g., a decrease of about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or more in the myeloid intrinsic gene score) identifies the individual as likely to respond to treatment with an RA therapeutic agent (e.g., an RA therapeutic agent including a DMARD such as methotrexate). In some cases, the method may further include administering an additional dose of an RA therapeutic agent to the individual. In some cases, a reduced or decreased myeloid intrinsic gene score refers to an overall decrease of at least about 1.1×, 1.2×, 1.3×, 1.4×, 1.5×, 1.6×, 1.7×, 1.8×, 1.9×, 2×, 2.1×, 2.2×, 2.3×, 2.4×, 2.5×, 2.6×, 2.7×, 2.8×, 2.9×, 3×, 3.5×, 4×, 4.5×, 5×, 6×, 7×, 8×, 9×, 10×, 15×, 20×, 30×, 40×, 50×, 100×, 500×, or 1000× as compared to a reference myeloid intrinsic gene score. In some cases, a reduced myeloid intrinsic gene score refers to an overall decrease in the myeloid intrinsic gene score of more than about 1.1 fold, about 1.2 fold, about 1.3 fold, about 1.4 fold, about 1.5 fold, about 1.6 fold, about 1.7 fold, about 1.8 fold, about 1.9 fold, about 2 fold, about 2.1 fold, about 2.2 fold, about 2.3 fold, about 2.4 fold, about 2.5 fold, about 2.6 fold, about 2.7 fold, about 2.8 fold, about 2.9 fold, about 3 fold, about 3.5 fold, about 4 fold, about 4.5 fold, about 5 fold, about 6 fold, about 7 fold, about 8 fold, about 9 fold, about 10 fold, about 15 fold, about 20 fold, about 30 fold, about 40 fold, about 50 fold, about 100 fold, about 500 fold, about 1,000 fold or more as compared to a reference myeloid intrinsic gene score.

[0188] In some cases, the method may further include, prior to administration of an RA therapeutic (e.g., an RA therapeutic including a DMARD such as methotrexate), (i) determining a first lymphoid-specific gene score from a sample from the individual at a first time point during or after administration of the DMARD, (ii) determining a second lymphoid-specific gene score from a sample from the individual at a second time point, and (iii) comparing the first lymphoid-specific gene score to the second lymphoid-specific gene score, wherein a decrease in the second lymphoid-specific gene score compared to the first lymphoid-specific gene score (e.g., a decrease of about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, or 99% or more in the lymphoid-specific gene score) identifies the individual as likely to respond to treatment with an RA therapeutic (e.g., an RA therapeutic including a DMARD such as methotrexate). In some cases, a reduced or decreased lymphoid-specific gene score refers to an overall decrease of at least about 1.1×, 1.2×, 1.3×, 1.4×, 1.5×, 1.6×, 1.7×, 1.8×, 1.9×, 2×, 2.1×, 2.2×, 2.3×, 2.4×, 2.5×, 2.6×, 2.7×, 2.8×, 2.9×, 3×, 3.5×, 4×, 4.5×, 5×, 6×, 7×, 8×, 9×, 10×, 15×, 20×, 30×, 40×, 50×, 100×, 500×, or 1000× as compared to a reference lymphoid-specific gene score. In some cases, a reduced lymphoid-specific gene score refers to an overall decrease in the lymphoid-specific gene score of more than about 1.1 fold, about 1.2 fold, about 1.3 fold, about 1.4 fold, about 1.5 fold, about 1.6 fold, about 1.7 fold, about 1.8 fold, about 1.9 fold, about 2 fold, about 2.1 fold, about 2.2 fold, about 2.3 fold, about 2.4 fold, about 2.5 fold, about 2.6 fold, about 2.7 fold, about 2.8 fold, about 2.9 fold, about 3 fold, about 3.5 fold, about 4 fold, about 4.5 fold, about 5 fold, about 6 fold, about 7 fold, about 8 fold, about 9 fold, about 10 fold, about 15 fold, about 20 fold, about 30 fold, about 40 fold, about 50 fold, about 100 fold, about 500 fold, about 1,000 fold or more as compared to a reference lymphoid-specific gene score.

[0189] In some cases of any of the above-mentioned methods of treatment, the decrease in the second myeloid-specific gene score compared to the first myeloid-specific gene score is about 1.25-fold to about 10-fold. In some cases, the decrease is about 1.25-fold to about 7.5-fold. In some cases, the decrease is about 1.25-fold to about 5-fold. In some cases, the decrease is about 1.25-fold to about 2-fold. In some cases, the decrease is about 1.25-fold to about 1.5-fold. In some cases, the decrease is at least about 1.25-fold.

[0190] In some cases of any of the above-mentioned methods of treatment, the decrease in the second lymphoid-specific gene score compared to the first lymphoid-specific gene score is about 1.25-fold to about 10-fold. In some cases, the decrease is about 1.25-fold to about 7.5-fold. In some cases, the decrease is about 1.25-fold to about 5-fold. In some cases, the decrease is about 1.25-fold to about 2-fold. In some cases, the decrease is about 1.25-fold to about 1.5-fold. In some cases, the decrease is at least about 1.25-fold.

[0191] In some cases of any of the above-mentioned methods of treatment, the reference myeloid intrinsic gene score can be a reference myeloid intrinsic gene score in a reference population of individuals with RA who have been previously treated with DMARDs, the population of individuals being comprised of a first subset of individuals who have responded to DMARD therapy and a second subset of individuals who have not responded to DMARD therapy.In some cases, the reference myeloid intrinsic gene score level significantly separates each of the first and second subsets of individuals based on a significant difference between the myeloid intrinsic gene score of the first subset of individuals compared to that of the second subset of individuals.In some cases, about 6 months after the start of DMARD therapy, the first subset of individuals responded to DMARD therapy and the second subset did not respond to DMARD therapy.

[0192] In some cases of any of the above-mentioned methods of treatment, the reference lymphoid intrinsic gene score can be a reference lymphoid intrinsic gene score in a reference population of individuals with RA who have been previously treated with DMARDs, the population of individuals being composed of a first subset of individuals who have responded to DMARD therapy and a second subset of individuals who have not responded to DMARD therapy.In some cases, the reference lymphoid intrinsic gene score level significantly separates each of the first and second subsets of individuals based on the significant difference of the lymphoid intrinsic gene score in the first subset of individuals compared with that of the second subset of individuals.In some cases, about 6 months after the start of DMARD therapy, the first subset of individuals responds to DMARD therapy, and the second subset does not respond to DMARD therapy.

[0193] In some cases of any of the preceding methods, the RA therapeutic agent may be a DMARD.

[0194] In some cases of any of the preceding methods, a RA therapeutic agent other than a DMARD may be administered.

[0195] In some cases of any of the preceding methods, the DMARD may be administered in combination with a therapeutic agent other than a DMARD.

[0196] In some instances of any of the preceding methods, the DMARDs may include, but are not limited to, methotrexate, hydroxychloroquine, sulfasalazine, lefunomide, azathioprine, cyclophosphamide, cyclosporine, and mycophenolate mofetil.

[0197] In some cases of any of the preceding methods, the therapeutic agent other than a DMARD may include, but is not limited to, a B cell antagonist, a JAK antagonist, a TNF antagonist, a decoy TNF receptor, a T cell costimulatory signal antagonist, an IL-1 receptor antagonist, an IL-6 receptor antagonist, or a combination thereof.

[0198] In some cases of any of the preceding methods, the B-cell antagonist can be, but is not limited to, rituximab (e.g., RITUXAN®).

[0199] In some cases of any of the preceding methods, the JAK antagonist can be, but is not limited to, tofacitinib (e.g., XELJANZ®).

[0200] In some cases of any of the preceding methods, the TNF antagonist can be, but is not limited to, adalimumab, golimumab, infliximab, certolizumab pegol, or a combination thereof.

[0201] In some cases of any of the preceding methods, the TNF decoy receptor can be, but is not limited to, etanercept.

[0202] In some cases of any of the preceding methods, the T cell costimulatory signal antagonist can be, but is not limited to, abatacept.

[0203] In some cases of any of the preceding methods, the IL-1 receptor antagonist can be, but is not limited to, anakinra.

[0204] In some cases of any of the preceding methods, the IL-6 receptor antagonist can be, but is not limited to, tocilizumab (e.g., ACTEMRA® / RoACTEMRA®).

[0205] In some cases of any of the preceding methods, the RA therapeutic agent utilized in the methods described herein can be administered, for example, orally, subcutaneously, intramuscularly, intravenously, intradermally, percutaneously, intraarterially, intraperitoneally, intralesionally, intracranially, intraarticularly, intraprostatically, intrapleurally, intratracheally, intrathecally, intranasally, intravaginally, intrarectally, topically, intratumorally, peritoneally, subconjunctivally, intravesicularly, mucosally, intrapericardially, intraumbilically, intraocularly, intraorbitally, intravitreally (e.g., by intravitreal injection), by eye drop, topically, transdermally, parenterally, by inhalation, by injection, by implantation, by infusion, by continuous infusion, by localized perfusion that directly bathes target cells, by catheter, by lavage, in cream, or in lipid composition.In some cases of any of the preceding methods, the RA therapeutic agent utilized in the methods described herein can be administered orally. In some cases of any of the preceding methods, the RA therapeutic agent utilized in the methods described herein can be administered subcutaneously. The RA therapeutic agent utilized in the methods described herein can also be administered systemically or locally. The method of administration can vary depending on various factors, such as the RA therapeutic agent to be administered and the severity of the condition, disease, or disorder (e.g., RA) to be treated.

[0206] III. Compositions and Pharmaceutical Formulations In one aspect, the invention is based, in part, on the discovery that the biomarkers of the invention can be used to identify individuals with RA who may benefit from a therapy including a RA therapeutic, e.g., a DMARD (e.g., a DMARD), or a biological therapeutic (e.g., a biological therapeutic) alone or in combination with a DMARD.

[0207] In some cases, an individual is unlikely to respond to DMARDs alone.In another case, the present invention is based in part on the discovery that the biomarkers of the present invention can be used to monitor and / or evaluate the treatment response of an individual with RA who is treated with RA therapeutic agents, including DMARDs.These agents and their combinations are useful for treating RA, for example, as part of any of the methods and uses described herein, for example, in Section II above.Any suitable DMARD can be used in the methods and uses described herein.

[0208] A. Pharmaceutical Formulations Therapeutic formulations of therapeutic agents used in accordance with the present invention, e.g., DMARDs and / or biological therapeutic agents, are prepared for storage in the form of a lyophilized formulation or aqueous solution by mixing the therapeutic agent having the desired degree of purity with optional pharma- ceutically acceptable carriers, excipients, or stabilizers. For general information regarding formulations, see, for example, Gilman et al. (eds.) The Pharmacological Bases of Therapeutics, 8th Ed., Pergamon Press, 1990; A. Gennaro (ed.), Remington's Pharmaceutical Sciences, 18th Edition, Mack Publishing Co., Pennsylvania, 1990; Avis et al. (eds.) Pharmaceutical Dosage Forms: Parenteral Medications Dekker, New York, 1993; Lieberman et al. (eds.) Pharmaceutical Dosage Forms: Tablets Dekker, New York, 1990; Lieberman et al. (eds.), Pharmaceutical Dosage Forms: Disperse Systems Dekker, New York, 1990; and Walters (ed.) Dermatological and Transdermal Formulations (Drugs and the Pharmaceutical Sciences), Vol 119, Marcel See Dekker, 2002.

[0209] Acceptable carriers, excipients, or stabilizers are nontoxic to recipients at the dosages and concentrations employed and include, but are not limited to, buffers such as phosphates, citrates, and other organic acids; antioxidants including ascorbic acid and methionine; preservatives (such as octadecyldimethylbenzyl ammonium chloride; hexamethonium chloride; benzalkonium chloride, benzethonium chloride; phenol, butyl, or benzyl alcohol; alkyl parabens such as methyl or propyl paraben; catechol; resorcinol; cyclohexanol; 3-pentanol; and m-cresol); low molecular weight (less than about 10 residues) polypeptides; serum albumin; hydrophilic polymers such as polyvinylpyrrolidone; amino acids such as glycine, glutamine, asparagine, histidine, arginine, or lysine; monosaccharides, disaccharides, and other carbohydrates, including glucose, mannose, or dextrins; chelating agents such as EDTA; sugars such as sucrose, mannitol, trehalose, or sorbitol; salt-forming counterions such as sodium; metal complexes (e.g., Zn-protein complexes); and / or non-ionic surfactants such as TWEEN™, PLURONICS™, or polyethylene glycol (PEG).

[0210] The formulations herein may also contain more than one active compound, preferably those with complementary activities that do not adversely affect each other. The type and effective amount of such medicaments will depend, for example, on the amount and type of therapeutic agent (e.g., DMARD and / or biological therapeutic agent) present in the formulation, as well as on the clinical parameters of the patient.

[0211] The active ingredient may also be encapsulated in microcapsules, for example, hydroxymethylcellulose or gelatin-microcapsules, and poly-(methylmethacylate) microcapsules, in colloidal drug delivery systems (e.g., liposomes, albumin microspheres, microemulsions, nanoparticles, and nanocapsules), or in macroemulsions, respectively, prepared, for example, by coacervation techniques or by interfacial polymerization. Such techniques are disclosed in Remington's Pharmaceutical Sciences 16th edition, Osol, A. Ed., 1980.

[0212] Sustained release preparations may be prepared.Suitable examples of sustained release preparations include semipermeable matrices of hydrophobic solid polymers containing therapeutic agents, and these matrices are in the form of shaped articles, such as films or microcapsules.Examples of sustained release matrices include polyesters, hydrogels (e.g., poly(2-hydroxyethyl-methacrylate) or poly(vinyl alcohol)), polylactides (U.S. Pat. No. 3,773,919), copolymers of L-glutamic acid and gamma-ethyl-L-glutamate, non-degradable ethylene-vinyl acetate, degradable lactic acid-glycolic acid copolymers such as LUPRON DEPOT™ (injectable microspheres made of lactic acid-glycolic acid copolymer and leuprolide acetate), and poly-D-(-)-3-hydroxybutyric acid.

[0213] Formulations to be used for in vivo administration must be sterile, which is readily accomplished by filtration through sterile filtration membranes.

[0214] IV. Products and Kits In another aspect of the invention, kits or articles of manufacture are provided that contain materials useful for the treatment, prevention, diagnosis, and / or monitoring of an individual.

[0215] In some cases, such kits or products can be used to identify individuals with RA who may benefit from RA therapeutics, including therapeutics other than or in addition to DMARDs (including, but not limited to, biological therapeutics alone or biological therapeutics and DMARDs). Such products or kits may include (i) a reagent(s) for determining the expression level of one or more genes listed in Table 1, or any combination thereof (e.g., any combination listed in any one of Tables 2 and 3) in a sample from an individual, and, optionally, (ii) an instruction(s) for using the reagent(s) to identify individuals with RA who may benefit from treatment with RA therapeutics, including therapeutics other than or in addition to DMARDs. In additional cases, the article of manufacture or kit may include (i) a reagent(s) for determining the expression level of one or more genes set forth in Table 1, or any combination thereof (e.g., any combination set forth in any one of Tables 2 and 3), in a sample from an individual, and, optionally, (ii) an instruction(s) for using the reagent(s) to monitor and / or assess the response of an individual having RA to treatment with a RA therapy, including a therapeutic agent other than or in addition to a DMARD (e.g., a biologic therapeutic agent alone, or a biologic therapeutic agent and a DMARD).

[0216] In some cases, such kits or products can be used to identify individuals with RA who may benefit from RA therapeutics, including therapeutics other than or in addition to DMARDs (including, but not limited to, biological therapeutics alone or biological therapeutics and DMARDs). Such products or kits may include (i) a reagent(s) for determining the expression level of one or more genes listed in Table 1, or any combination thereof (e.g., any combination listed in any one of Tables 2 and 3) in a sample from an individual, and (ii) an instruction(s) for using the reagent(s) to identify individuals with RA who may benefit from treatment with RA therapeutics, including therapeutics other than or in addition to DMARDs. In additional cases, the article of manufacture or kit may include (i) a reagent(s) for determining the expression level of one or more genes set forth in Table 1, or any combination thereof (e.g., any combination set forth in any one of Tables 2 and 3), in a sample from an individual, and (ii) an instruction(s) for using the reagent(s) to monitor and / or assess the response of an individual having RA to treatment with a RA therapy, including a therapeutic agent other than or in addition to a DMARD (e.g., a biologic therapeutic agent alone, or a biologic therapeutic agent and a DMARD).

[0217] Provided herein are kits or articles of manufacture for identifying individuals with RA who may benefit from treatment with a therapeutic agent other than or in addition to a DMARD (e.g., a biological therapeutic agent alone or a biological therapeutic agent and a DMARD), the kits or articles of manufacture comprising: (i) detecting in a sample from the individual one or more of the genes set forth in Table 1 (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106 , 18, 19, 20, 21, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, or 46) and, optionally, (ii) instructions for using the reagents to identify individuals with RA that may benefit from treatment with a RA therapeutic agent, including therapeutic agents other than or in addition to DMARDs.

[0218] For example, provided herein is a kit or article of manufacture for identifying an individual having RA who may benefit from treatment with a RA therapeutic (e.g., a biological therapeutic alone or a biological therapeutic and a DMARD), the kit or article of manufacture including (i) reagents for determining the expression level of one or more of the following genes (e.g., one, two, three, four, five, six, or seven of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A) in a sample from the individual, and, optionally, (ii) instructions for using the reagents to identify an individual having RA who may benefit from treatment with a RA therapeutic, including a therapeutic agent other than or in addition to a DMARD.

[0219] In any of the preceding cases, the kit may include reagents for determining expression levels of one or more (e.g., 1, 2, 3, 4, 5, 6, or 7) of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A. In some embodiments, the kit includes reagents for determining expression levels of at least two, at least three, at least four, at least five, at least six, or all seven of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A. In some embodiments, the kit includes reagents for determining expression levels of two of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, e.g., any of the exemplary combinations shown in Table 4. In some embodiments, the kit includes reagents for determining the expression levels of three of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, e.g., any of the exemplary combinations shown in Table 5. In some embodiments, the kit includes reagents for determining the expression levels of four of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, e.g., any of the exemplary combinations shown in Table 6. In some embodiments, the kit includes reagents for determining the expression levels of five of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, e.g., any of the exemplary combinations shown in Table 7. In some embodiments, the kit includes reagents for determining the expression levels of six of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, e.g., any of the exemplary combinations shown in Table 8.In some embodiments, the kit comprises reagents for determining expression levels of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, and, optionally, (ii) instructions for using the reagents to identify individuals with RA who may benefit from treatment with RA therapeutics, including therapeutic agents other than or in addition to DMARDs.

[0220] In some cases, such kits or articles of manufacture can be used to identify individuals with RA who may benefit from RA therapeutics, including DMARDs (including, but not limited to, DMARDs and / or biologics). Such articles of manufacture or kits may include (i) reagents for determining myeloid intrinsic genes, or myeloid intrinsic genes and lymphoid intrinsic genes score(s) in a sample from an individual, and, optionally, (ii) instructions for using the reagents to identify individuals with RA who may benefit from treatment with RA therapeutics, including DMARDs.

[0221] In additional cases, the article of manufacture or kit may include (i) reagents for determining myeloid intrinsic genes, or myeloid intrinsic genes and lymphoid intrinsic genes score(s) in a sample from an individual, and, optionally, (ii) instructions for using the reagents to monitor and / or assess the response of an individual with RA to treatment with RA therapies (e.g., DMARDs and / or biologic therapeutics), including DMARDs.

[0222] Provided herein are kits or articles of manufacture for identifying individuals with RA who may benefit from treatment with an RA therapy (e.g., a biologic agent alone or a biologic agent and a DMARD), the kits or articles of manufacture comprising: (i) detecting the presence or absence of one or more of the genes set forth in Table 1 (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, , 19, 20, 21, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, or 46) and (ii) instructions for using the reagents to identify individuals with RA who may benefit from treatment with a RA therapeutic agent, including therapeutic agents other than or in addition to DMARDs.

[0223] For example, provided herein is a kit or article of manufacture for identifying individuals with RA who may benefit from treatment with a RA therapeutic (e.g., a biological therapeutic alone, or a biological therapeutic and a DMARD), the kit or article of manufacture including (i) reagents for determining the expression level of one or more (e.g., 1, 2, 3, 4, 5, 6, or 7) of the following genes in a sample from the individual: CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, and (ii) instructions for using the reagents to identify individuals with RA who may benefit from treatment with a RA therapeutic, including therapeutic agents other than or in addition to DMARDs.

[0224] In any of the preceding cases, the kit may include reagents for determining expression levels of one or more (e.g., 1, 2, 3, 4, 5, 6, or 7) of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A. In some embodiments, the kit includes reagents for determining expression levels of at least two, at least three, at least four, at least five, at least six, or all seven of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A. In some embodiments, the kit includes reagents for determining expression levels of two of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, e.g., any of the exemplary combinations shown in Table 4. In some embodiments, the kit includes reagents for determining the expression levels of three of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, e.g., any of the exemplary combinations shown in Table 5. In some embodiments, the kit includes reagents for determining the expression levels of four of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, e.g., any of the exemplary combinations shown in Table 6. In some embodiments, the kit includes reagents for determining the expression levels of five of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, e.g., any of the exemplary combinations shown in Table 7. In some embodiments, the kit includes reagents for determining the expression levels of six of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, e.g., any of the exemplary combinations shown in Table 8. In some embodiments, the kit includes reagents for determining expression levels of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, as well as instructions for using the reagents to identify individuals with RA who may benefit from treatment with RA therapeutics, including therapeutic agents other than or in addition to DMARDs.

[0225] In some cases, such kits or articles of manufacture can be used to identify individuals with RA who may benefit from RA therapeutics, including DMARDs (including, but not limited to, DMARDs and / or biologics). Such articles of manufacture or kits may include (i) reagents for determining myeloid intrinsic genes, or myeloid intrinsic genes and lymphoid intrinsic genes score(s) in a sample from an individual, and (ii) instructions for using the reagents to identify individuals with RA who may benefit from treatment with RA therapeutics, including DMARDs.

[0226] In additional cases, the article of manufacture or kit may include (i) reagents for determining myeloid intrinsic genes, or myeloid intrinsic genes and lymphoid intrinsic genes score(s) in a sample from an individual, and (ii) instructions for using the reagents to monitor and / or assess the response of an individual with RA to treatment with RA therapies (e.g., DMARDs and / or biologic therapeutics), including DMARDs.

[0227] Provided herein are kits or articles of manufacture for treating an individual with RA, the kits or articles of manufacture comprising: (i) a biologic therapeutic agent alone, or a biologic therapeutic agent and a DMARD; and (ii) an expression level of one or more of the genes set forth in Table 2 (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 11 In one embodiment, the method comprises administering an effective amount of a biological therapeutic alone, or a biological therapeutic and a DMARD to the individual to treat or slow the progression of RA based on a determination of increased expression levels of one or more of the following:

[0228] Provided herein is a kit or article of manufacture for treating an individual with RA, the kit or article of manufacture comprising: (i) a biological therapeutic agent alone, or a biological therapeutic agent and a DMARD; and (ii) instructions for administering to the individual an effective amount of the biological therapeutic agent alone, or a biological therapeutic agent and a DMARD to treat or delay the progression of RA in the individual, based on a determination of increased expression levels of one or more of the following genes in a sample from the individual (e.g., one, two, three, four, five, six, or seven of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A) compared to a reference expression level.

[0229] In some embodiments, the kit comprises instructions for administering to the individual an effective amount of a biologic therapeutic alone, or a biologic therapeutic and a DMARD, to treat or delay the progression of RA in the individual based on determination of increased expression levels of at least two, at least three, at least four, at least five, at least six, or all seven of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A in a sample from the individual compared to a reference expression level.

[0230] In some embodiments, the kit includes instructions for administering to the individual an effective amount of a biologic therapeutic alone, or a biologic therapeutic and a DMARD, to treat or delay the progression of RA in the individual based on a determination of increased expression levels of two of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, such as any of the exemplary combinations shown in Table 4, in a sample from the individual compared to a reference expression level.

[0231] In some embodiments, the kit comprises instructions for administering to the individual an effective amount of a biologic therapeutic alone, or a biologic therapeutic and a DMARD, to treat or delay the progression of RA in the individual based on a determination of increased expression levels of three of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, such as any of the exemplary combinations shown in Table 5, in a sample from the individual compared to a reference expression level.

[0232] In some embodiments, the kit includes instructions for administering to the individual an effective amount of a biologic therapeutic alone, or a biologic therapeutic and a DMARD, to treat or delay the progression of RA in the individual based on a determination of increased expression levels of any of four of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, such as exemplary combinations shown in Table 6, in a sample from the individual compared to a reference expression level.

[0233] In some embodiments, the kit includes instructions for administering to the individual an effective amount of a biologic therapeutic alone, or a biologic therapeutic and a DMARD, to treat or delay the progression of RA in the individual based on a determination of increased expression levels of any of five of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, such as exemplary combinations shown in Table 7, in a sample from the individual compared to a reference expression level.

[0234] In some embodiments, the kit includes instructions for administering to the individual an effective amount of a biologic therapeutic alone, or a biologic therapeutic and a DMARD, to treat or delay the progression of RA in the individual based on a determination of increased expression levels of any of six of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A, such as exemplary combinations shown in Table 8, in a sample from the individual compared to a reference expression level.

[0235] In some embodiments, the kit comprises instructions for administering to the individual an effective amount of a biological therapeutic alone, or a biological therapeutic and a DMARD, to treat or delay the progression of RA in the individual based on determination of increased expression levels of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A in a sample from the individual compared to a reference expression level.

[0236] Provided herein is a kit or article of manufacture for treating an individual with RA, the kit or article of manufacture comprising: (i) an RA therapeutic agent (e.g., a biological therapeutic agent alone, or a biological therapeutic agent and a DMARD); and (ii) instructions for administering to the individual an effective amount of the biological therapeutic agent alone, or a biological therapeutic agent and a DMARD to treat or delay the progression of RA in the individual, based on a determination of a decreased expression level of one or more of the genes set forth in Table 3 (e.g., one, two, three, four, five, or six of the genes set forth in Table 3) in a sample from the individual compared to a reference expression level.

[0237] Provided herein are kits or articles of manufacture for treating an individual with RA, the kits or articles of manufacture including (i) an RA therapeutic agent (e.g., a DMARD and / or a biological therapeutic agent), and (ii) instructions for administering to the individual an effective amount of the RA therapeutic agent to treat or delay the progression of RA in the individual based on determination of an increased myeloid intrinsic gene score in a sample from the individual compared to a reference myeloid intrinsic gene score.

[0238] Provided herein are kits or articles of manufacture for treating an individual with RA, the kits or articles of manufacture comprising (i) an RA therapeutic agent (e.g., a DMARD and / or a biological therapeutic agent), and (ii) instructions for administering to the individual an effective amount of the RA therapeutic agent to treat or delay the progression of RA in the individual based on determination of an increased myeloid intrinsic gene score and an increased lymphoid intrinsic gene score in a sample from the individual compared to a reference myeloid intrinsic gene score and a reference lymphoid intrinsic gene score.

[0239] Any of the kits or articles of manufacture described may include a carrier means that is compartmentalized to receive in close confinement one or more container means, such as vials, tubes, and the like, each of which contains one of the separate elements to be used in the method. Where the article or kit utilizes nucleic acid hybridization to detect target nucleic acids, the kit may also have a container housing a nucleotide(s) for amplification of the target nucleic acid sequence and / or a container containing a reporter means, such as an enzymatic, florescent, or radioisotope label.

[0240] In some cases, the product or kit includes the container described above and one or more other containers that contain materials that are desirable from a commercial and user perspective, including buffers, diluents, filters, needles, syringes, and package inserts that include instructions for use. A label may also be present on the container to indicate that the composition is to be used in a particular application, and may indicate instructions for either in vivo or in vitro use, such as those described above. For example, the product or kit may further include a container that contains a pharma- ceutically acceptable buffer, such as bacteriostatic water for injection (BWFI), phosphate buffered saline, Ringer's solution, and dextrose solution.

[0241] The kit or product described herein may have several cases. In one case, the kit or product includes a container, a label on the container, and a composition contained within the container, the composition includes one or more polynucleotides that hybridize under stringent conditions to the complementary sequence of a gene listed herein (e.g., a gene listed in Table 1, or any combination of genes listed in Tables 2 and 3), the label on the container indicates that the composition can be used to evaluate the presence of a gene listed herein (e.g., a gene listed in Table 1, or any combination of genes listed in Tables 2 and 3) in a sample, and the kit includes instructions for using the polynucleotide(s) to evaluate the presence of gene RNA or DNA in a particular sample type.

[0242] In the case of an oligonucleotide-based product or kit, the product or kit may, for example, include (1) an oligonucleotide, e.g., a detectably labeled oligonucleotide, that hybridizes to a nucleic acid sequence encoding a protein, or (2) a pair of primers useful for amplifying a nucleic acid molecule. The product or kit may also include, for example, a buffer, a preservative, or a protein stabilizer. The product or kit may further include components (e.g., an enzyme or a substrate) necessary for detecting the detectable label. The product or kit may also contain a control sample or a series of control samples that may be assayed and compared to the test sample. Each component of the product or kit may be enclosed within an individual container, and all of the various containers may be in a single package, along with instructions for interpreting the results of the assay performed using the kit. EXAMPLES

[0243] V. Working Examples Below are examples of methods of the present invention: In view of the general description provided above, it will be understood that various other embodiments may be practiced.

[0244] Example 1. Association between immunohistochemical (IHC) pathotypes and gene expression levels in individuals with rheumatoid arthritis (RA) Ultrasound-guided synovial biopsy samples were collected from a cohort of 129 individuals enrolled in the Pathobiology of Early Arthritis Cohort (PEAC), a treatment-naïve population of early rheumatoid arthritis (RA) patients with symptoms of less than 12 months duration. Histological analysis and gene expression microarray experiments were performed on isolated synovial tissue to identify pathotype-specific cellular and gene expression markers, evaluate associations between gene expression patterns and disease progression, and identify potential biomarkers for prognostic and predictive purposes.

[0245] research design One hundred and forty-four individuals with RA who met the American College of Rheumatology (ACR) / European League Against Rheumatism (EULAR) 2010 RA classification criteria were enrolled at Barts Health National Health Service (NHS) Trust as part of the Pathobiology of Early Arthritis Cohort (PEAC), a multicentre study funded by the Medical Research Council (MRC). The study received local ethical approval and all individuals provided written informed consent. Individuals presented with clinically defined synovitis but symptoms lasted less than 12 months. Characteristics of study subjects are summarised in Figure 1A. Briefly, the mean DAS28-ESR score was 5.6 (standard deviation 1.5), approximately 65% ​​were positive for rheumatoid factor and / or anti-citrullinated peptide antibodies (ACPA), and 20% had at least one radiographic erosion. All individuals were disease-modifying antirheumatic drug (DMARD) and steroid therapy naïve. Upon enrollment and acquisition of demographic characteristics and clinical disease parameters, individuals underwent minimally invasive ultrasound (US)-guided synovial biopsies of clinically active joints (Figure 1B). The majority of biopsied joints in this cohort were from the wrist (approximately 65%), with an additional component from the MCP / PIP / MTP joints (approximately 15%), while knees and elbows together constituted approximately 20% (Figure 1C). Individuals were then started on standard DMARD therapy (methotrexate (MTX), sulfasalacine (SSZ), and / or hydroxychloroquine (HCQ)) and / or low-dose steroids (intramuscular or oral). A treat-to-target approach to treatment escalation was followed, with the goal of low disease activity, i.e., a disease activity score in 28 joints (DAS28) < 3.2. For individuals failing DMARD therapy, biologic therapy was initiated according to the UK National Institute for Clinical Excellence (NICE) defined algorithm for individuals with RA if they continued to have a DAS28>5.1 at 6 months.After 6 months of treatment, a second synovial biopsy (unless clinically contraindicated) was performed on the same joint along with an assessment of disease activity. At the time of biopsy, ultrasound scores were collected for both the individual joint scores that underwent biopsy as well as a global joint score. Just before baseline, standard longitudinal images of the first to fifth metacarpophalangeal (MCP) joints in US-guided synovial biopsies and midline, radial, and ulnar views of both wrist joints were obtained in addition to standard images of the joints that underwent US-guided synovial biopsies. A blinded assessor (IL) then performed a semiquantitative (SQ) assessment of the images for activity by both synovial thickening (ST) and power Doppler (PD) according to the standard US synovitis score (grade 0-3) according to EULAR, Outcome Measures in Rheumatology (OMERACT). For each individual, baseline total mean (12 max) ST (STUS) and PD (PDUS) scores were calculated by deriving the mean of the total ST and PD scores for all 12 joints, including the max score at the wrist. STUS and PDUS of biopsied joints were also recorded. Anonymized plain radiographs of the hands and feet were chronologically scored by trained readers according to the van der Heijde modified Sharp score (ShSS) at baseline and at 12 months follow-up.

[0246] Identification of synovial pathotype based on histology To classify individuals into disease pathotypes, synovial biopsy tissue samples were evaluated by immunohistochemistry. A minimum of six biopsies were paraffin embedded and 3 μM sections were subjected to routine hematoxylin and eosin (H&E) staining and were graded for morphology and sample integrity. Synovial tissue samples from 129 of the 144 individuals recruited met the sample integrity criteria and were processed for further analysis. To determine the extent of immune cell infiltration, serially cut sections were stained for B cells (CD20), T cells (CD3), macrophages (CD68), and plasma cells (CD138). Sections were then SQ scored (0-4) for the number of CD3, CD20, CD68 inner (CD68l) and subinner (CD68sl), and CD138. The presence of CD20+ aggregates was noted within the synovial tissue, and the aggregates were graded (1–3) according to a previously published scoring system (Humby et al. PLos Medicine 6(1):e1, 2009).

[0247] Biopsy samples were then stratified into one of three synovial pathotypes according to the following criteria: (i) lymphoid: presence of grade 2-3 CD20+ aggregates (CD20 ≥ 2), and / or CD138 > 2, (ii) myeloid: CD68 SL ≥ 2, CD20 ≤ 1, and / or CD3 ≥ 1, CD138 ≤ 2, and (iii) microimmuno-fibrotic: CD68 SL < 2, and CD3, CD20, and CD138 < 1. Representative photomicrographs are shown in Figure 2A. Overall, 39% of individuals had a lymphoid pathology characterized by an abundance of immune cells (T cells, B cells, and plasma cells) (n=51), 34% had a myeloid pathology characterized by a predominance of macrophages and T cells but few B cells and plasma cells (n=44), and 27% had a minimal immune-fibrotic pathology characterized by a proliferation of fibroblasts but very few immune cells (n=34) (Figure 2B).

[0248] Identification of pathotype-specific gene expression markers To identify genes that were differentially expressed between the three major pathotype clusters, total RNA was extracted from synovial tissue collected from a subset of PEAC individuals. Synovial samples were homogenized in TRIzol reagent (ThermoFisher Scientific, Life Technologies, Invitrogen Division, UK) using an isostatic homogenizer after shearing with a 26-gauge needle. Total RNA was isolated according to the manufacturer's protocol and stored at -80°C. All RNA samples were quantified using spectrophotometric analysis performed on a NanoDrop-ND2000C system (Lab Tech, UK). RNA integrity was determined by RNA Nanochip electrophoresis on an Agilent 2100 Bioanalyzer system (Agilent Technologies, UK). After confirming RNA integrity, 1 μg of total RNA (when available) was used for library preparation using the TruSeq RNA Sample Preparation Kit Version 2 (Illumina). The resulting libraries were first amplified by 10 cycles of PCR, the library sizes were confirmed using a 2200 TapeStation and High Sensitivity D1K Screen Tape (Agilent Technologies), and their concentrations were determined by a qPCR-based method using a Library Quantification Kit (KAPA). The libraries were first multiplexed (5 per lane) and then sequenced on an Illumina HiSeq2500 (Illumina) to generate 50 million paired-end 75 bp reads.

[0249] RNA sequencing data processing and analysis was performed using the R programming language with packages from the Bioconductor Project. Raw RNA sequencing reads were processed using the HTSeqGenie Bioconductor package (version 4.0.1). Briefly, reads were aligned to the reference human genome sequence (build 38) using the GSNAP algorithm and the following parameters: -M2 -n10 -B2 -i1 -N1 -w200000 -E1 -pairmax-rna =200000 -clip-overlap. Uniquely aligned read pairs falling within an exon were counted to obtain estimates of expression levels for individual genes. Data were further normalized using a variance-stabilizing transformation implemented in the DESeq2 Bioconductor package.

[0250] To identify pathotype-specific genes, differential expression analysis was performed for pathotypes classified in a previous microarray study (GEO accession number GSE48780, Dennis et al. Arthritis Research and Therapy. 16(2):R90, 2014). Pairwise comparisons were performed between samples of minor immune-fibrotic, myeloid, and lymphoid pathotypes. Genes were selected for a pathotype if they showed differential expression for that pathotype with each of the other two pathotypes at a Benjamini-Hochberg adjusted p-value <0.01. These genes were then queried in baseline RNA-seq data from a subset of 90 individuals from the PEAC cohort.

[0251] For each set of pathotype-specific genes, 50 genes were identified that best correlated with the first principal component of the z-score-transformed expression data for that gene set. An additional set of 87 genes previously implicated in RA pathobiology was also included. Expression levels determined by NANOSTRING® were concordant with those measured by RNA sequencing (in samples where both measurements were available) (Figure 2C). Analysis of NANOSTRING® expression data was performed using R version 3.3.2. For differential expression analysis, the limma Bioconductor package was used with default settings. The Benjamini-Hochberg method was used to adjust for multiple testing, and genes were considered differentially expressed if they had an adjusted p-value <0.01. More detailed examination of the myeloid pathotype markers identified two clusters of genes, with high correlation within each cluster, but only moderate correlation between the clusters. The larger cluster, containing known myeloid cell expressed markers including CD86, PILRA, and C5AR1, was selected as the marker gene set for myeloid pathotype, reducing the myeloid gene set to 26 genes. The final NANOSTRING® dataset contained 212 probes: 49 lymphoid-specific genes (Table 9), 26 myeloid-specific genes (Table 10), 50 trace immune-fibrotic specific genes (Table 11), and 87 RA biology-related genes (Table 12). Table 9. Lymphoid-specific genes TIFF0007652566000012.tif255170TIFF0007652566000013.tif73170Table 10. Myeloid-specific genes TIFF0007652566000014.tif185170Table 11. Microimmunotype-fibrotic specific genes TIFF0007652566000015.tif255170TIFF0007652566000016.tif79170Table 12. RA biology-related genes TIFF0007652566000017.tif255170TIFF0007652566000018.tif54170

[0252] Pathogen-specific intrinsic gene scores predict immunohistochemistry (IHC)-determined pathotypes Eigengene scores were calculated as previously described (see, e.g., Bueno et al., Nature Genetics. 48(4):407-16, 2016, which is incorporated by reference in its entirety).

[0253] Gene expression values ​​were compressed into a single score per sample. A positive correlation was observed between synovial histological grade and lymphoid or myeloid specific gene scores, and in contrast, a negative correlation was observed between pauciimune-fibrotic specific gene scores and synovial histological grade (Figure 2D). A machine learning technique, k-nearest neighbors, was utilized to develop predictors of IHC-determined pathotypes using pathotype-specific specific genes. A 5-fold cross-validation approach predicted IHC pathotypes with 64% accuracy (kappa=0.44). In general, lymphoid pathotypes were more easily predicted than myeloid or pauciimune-fibrotic, and most misclassifications occurred between pauciimune-fibrotic and myeloid. This suggests that while molecular phenotyping may approximate IHC pathotypes, each technique yields complementary information about the sample.

[0254] Unsupervised clustering of NANOSTRING® expression data showed strong grouping of genes defined by pathotype, consistent with their initial pathotype assigned by histology (Figure 3A). Samples classified as either lymphoid or trace immune-fibrotic showed the highest expression scores of lymphoid or trace immune-fibrotic specific genes, respectively (Figure 3B). Samples classified as myeloid had intermediate levels of myeloid specific gene scores, with higher expression than trace immune-fibrotic samples, but lower expression than lymphoid samples (Figures 3B and 3C). Furthermore, comparison of gene expression across multiple pathotypes using one-way ANOVA revealed that nearly all genes measured were significantly different across the three groups. All but one of the 212 genes had an adjusted p-value <0.01 (Figure 3D).

[0255] The pauciimune-fibrotic specific gene score was inversely correlated with the lymphoid and myeloid specific gene scores, whereas the lymphoid and myeloid specific gene scores were positively correlated with each other (Figure 4A). Comparing myeloid pathotype samples with either lymphoid or pauciimune-fibrotic pathotype samples, there were fewer differentially expressed genes and lymphoid specific genes were less expressed in these samples compared to the combined lymphoid / pauciimune-fibrotic group (Figure 4B). This is consistent with the scoring algorithm used to determine pathotype, i.e., myeloid samples differ from lymphoid samples primarily by the relative lack of B cell staining.

[0256] Collectively, these data demonstrate that pathotype-specific gene sets show strong association with concordant clinically and immunohistochemically determined pathotypes.

[0257] Example 2. Associations between pathotypes, gene expression levels, clinical covariates, disease activity, and disease progression in individuals with RA Synovial pathology and gene expression levels were evaluated for their association with clinical covariates to determine whether they were predictive of disease activity and / or progression in individuals with RA.

[0258] Synovial pathology and gene expression predict radiographic progression Baseline pathology or gene expression was assessed for association with ongoing structural damage as measured by Sharp-van der Heijde radiographic progression score (ShSS) 12 months after initial biopsy. Although baseline pathology was not predictive of erosions or joint space narrowing at 12 months, lymphoid individuals showed a significantly higher increase in ShSS and were at higher risk of disease progression (ΔShSS >1) compared with myeloid and microimmune-fibrotic individuals (Figure 5A). Importantly, of the 16 individuals who subsequently initiated biologic therapy during 6-12 months of follow-up, a higher proportion were lymphoid (22.8%, 8 / 35) compared with myeloid / microimmune-fibrotic (13.8%, 8 / 58) groups (Fisher's exact test, p=0.27). Thus, despite more intensive treatment regimens (including higher use of biologic therapeutic agents), individuals with lymphocytic pathogenesis were significantly more likely to develop progression of joint damage.

[0259] To examine genes associated with subsequent radiographic progression, ShSS progressors and non-progressors were compared in terms of pre-treatment gene expression (Figure 5B). Forty-six genes with p-values ​​<0.05 were identified, including the B-cell-associated genes CD19, FCRL5, and BCMA. A complete list of these genes can be found in Table 1. Pre-treatment lymphoid specific gene scores were significantly elevated in individuals who had elevated ShSS scores at 12 months compared to those who did not (Figure 5C). In contrast, myeloid and trace immune-fibrotic specific gene scores were not elevated in progressors compared to non-progressors (Figure 5C). Additionally, gene sets previously defined (from datasets in the Harmonizome database) as expressed in osteoclasts were most highly expressed in lymphoid versus myeloid and fibrotic patients. The overall osteoclast gene set was significantly enriched in lymphoid patients versus other patients (Figure 5D), suggesting that bone resorption mechanisms and high B cell infiltration are occurring simultaneously in these patients, possibly as a result of highly favorable conditions for osteoclastogenesis in the synovial lining. In contrast, non-progressors had elevated levels of trace immune-fibrotic related genes, including FGF family members (e.g., Noggin) and cartilage intermediate layer proteins at baseline. Furthermore, osteoprotegerin, a decoy receptor for receptor activator of nuclear factor kappa B ligand (RANKL) and thus a negative regulator of osteoclastogenesis, was also elevated in non-progressors.

[0260] To determine whether baseline clinical and gene expression data could be combined into a model to predict radiographic progression, two complementary approaches were employed: (1) logistic regression coupled with backward model selection to identify a minimal set of clinical predictors, and (2) a penalized method based on logistic regression performed with the glm procedure from the R statistical package with an L1 regularization penalty (LASSO) to identify genes that improve the clinical model.

[0261] Logistic regression with backward stepwise model selection was applied to baseline clinical parameters for the dependent variable of radiographic progression at 12 months to select which clinical covariates contributed most to prediction. Sixteen baseline clinical covariates were considered as candidates in the regression model. Baseline variables included sex, age, disease duration, ESR, CRP, RF titer, ACPA titer (as continuous variables), VAS, tender and swollen joint counts, baseline DAS28-ESR, EULAR response at 6 months (categorical), baseline HAQ, USST and USPD 12 max scores, and baseline pathology (lymphoid vs. oligoimmune-fibroid / myeloid 2 categories). Stepwise variable selection resulted in a model with the following eight clinical variables: baseline RF titer, disease duration, VAS, swollen joint count, DAS28-ESR, baseline disease type, and 12 maximum scores of USST and USPD. LASSO was then applied to these eight clinical covariates and the 46 genes identified as significantly differentially expressed between progressors and non-progressors to determine the best sparse prediction model. The predictive performance of these models with genes alone, clinical covariates alone, and combinations of genes and clinical covariates was assessed by calculating the area under the receiver operating characteristic curve (AUC). The AUC represents the probability that for any randomly selected pair of individuals with and without radiographic progression, the predicted risk of the individual with radiographic progression was higher. A value of 0.50 represents no discrimination, and 1 represents perfect discrimination. Both apparent and internal validation were assessed. The apparent predictive performance of the model, assessed by the area under the curve (AUC), was 0.85 (95% CI: 0.71–0.98) for clinical covariates alone and 0.91 (% CI 0.79–1.0) for genes alone (Figure 5E).

[0262] A model incorporating RF titers and expression of seven genes included in Table 2 (CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A) yielded a predictor of progression with a lambda ratio of 0.0631 (Figure 6A). This model yielded improved apparent predictive performance of the model (AUC 0.93, 95% CI: 0.86 to 1, Figures 5E and 6B). Bootstrap resampling was performed to correct the AUC for potential overfitting. To perform the bootstrap, a random sample was drawn with restoration from the original sample. First, the model was trained on the bootstrap sample and validated on the original sample, and an AUC statistic was calculated. Then, the model was trained and validated on the same bootstrap sample, and another AUC statistic was calculated. The difference between the two AUCs was calculated, and this procedure was repeated 500 times. The 500 differences were then averaged to obtain an estimate of the overestimation bias (optimism). Overestimation bias-corrected AUC estimates were calculated as the apparent AUC minus the overestimation bias estimate. The overestimation bias-corrected AUC was 0.85 for the purely clinical model, 0.91 for the purely gene expression model, and 0.93 for the combined clinical and gene expression models, suggesting that while examination of gene expression or clinical covariates alone yielded reliable prognostic models, including both clinical covariates and genes (e.g., CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A) in the model yielded prognostic models with improved discrimination between individuals with and without radiographic progression compared to prognostic models that only considered clinical covariates or gene expression alone.

[0263] Synovial lymphoid pathology and myeloid gene expression scores correlate with overall RA disease activity Comparison of baseline disease activity characteristics across synovial pathology types did not identify any significant differences in age or disease duration across these groups (Figure 7A). However, lymphoid pathology had the highest levels of ESR, CRP, ACPA titers, swollen joint count, and DAS28-ESR scores. Radiographic assessment of joint damage showed a trend toward more severe joint space narrowing in the lymphoid group. Furthermore, ultrasound assessment showed that the lymphoid group had significantly higher levels of synovial thickening and power Doppler (PD) scores (both in biopsied joints and overall PD scores), indicating the presence of synovitis and suggesting a clinical association with active disease. In contrast, the microimmune-fibrotic group had the lowest levels of acute phase reactants, RF and ACPA positivity, and PD ultrasound scores, despite the presence of active disease, as determined clinically by DAS28-ESR, swollen joint counts, HAQ and VAS scores, and elevated synovitis determined by ultrasound.

[0264] The expression scores of each eigengene were compared to clinical covariates of disease activity, including DAS28-ESR score, ESR, CRP, swollen and tender joint counts, visual analog scale, HAQ DI score, and ultrasound, both in biopsied joints and overall, using Spearman's rank-sum correlation (Figure 7B). The myeloid eigengene score was highly associated with many aspects of disease severity, including ESR and CRP, joint counts, DAS28-ESR score, HAQ DI score, and overall PD ultrasound score. The lymphoid eigengene score also correlated with many of these, but at lower levels and more with ultrasound (both power Doppler and synovial thickening scale) in biopsied joints. As expected based on gene expression correlations, the trace immune-fibrotic eigengene score was negatively associated with many aspects of disease activity.

[0265] Taken together, these data show that baseline pathotype or gene expression levels, alone or in combination with clinical covariates, can serve as prognostic biomarkers that are predictive of disease progression in individuals with RA.As a result, the assessment of baseline pathotype and gene expression levels, alone or in combination with clinical covariates, can be used to identify individuals with RA who may benefit from treatments that include therapeutic agents other than or in addition to DMARDs (e.g., biological therapeutic agents).These results also suggest that overall disease activity in individuals is most closely related to the expression of myeloid-related genes in joints, while elevated trace immune-fibrotic genes are associated with lower disease severity across a range of disease activity parameters.

[0266] Example 3. Association between myeloid and lymphoid intrinsic gene scores and response to DMARD therapy As discussed in Example 2, the pathotype-specific eigengene scores correlated with disease activity and progression. The pathotype-specific eigengene scores were further evaluated for their association with an individual's responsiveness to DMARD therapy to determine whether they could serve as biomarkers for predicting and / or monitoring responsiveness to DMARD therapy.

[0267] Association of pretreatment pathotype and gene expression levels with response to DMARD treatment Individuals with three distinct histologically determined pathotypes were compared with respect to changes in DAS28-ESR at 6 months and individual response to DMARD therapy as determined by EULAR response criteria. No significant associations between baseline pathotype status and treatment outcome were observed, but it was notable that individuals with myeloid and lymphoid pathotypes were more often treated with combinations of methotrexate and other DMARDs, consistent with goal-directed treatment strategies used in individuals with more aggressive disease (Figure 8A). Examination of gene expression differences between baseline and 6 months in individuals who achieved a good EULAR response showed that multiple inflammatory pathways were reduced, including genes associated with lymphoid aggregates (e.g., CCL19, BTLA, IL21R, CXCL13, LTA, and LTB) and inflammatory cytokines (e.g., IL6) (Figure 8B). In contrast, non-responders showed smaller decreases in inflammatory gene expression, indicative of synovitis (Figure 8C).

[0268] The eigengene scores at baseline were then assessed for association with treatment outcome after DMARD therapy. Higher expression of myeloid and lymphoid (but not microimmuno-fibrotic) eigengenes was associated with a greater decrease in DAS28-ESR scores after treatment (Spearman's rho = -0.3 (M), p = 0.003; rho = -0.21 (L), p = 0.044) (Figure 8D).

[0269] To determine whether the pathotype-specific gene score was affected by DMARD therapy, baseline and 6-month samples were compared separately for individuals who achieved a good response to DMARD therapy and those who did not. Individuals who showed a good response to DMARD therapy had highly dynamic gene expression, with a significant decrease in both lymphoid and myeloid specific gene scores, and a concomitant increase in the trace immune-fibrotic specific gene score (Figure 8E). Non-responders exhibited a more muted change in gene expression, with a significant decrease in lymphoid specific gene score, but with a wide variation in the changes in the myeloid and trace immune-fibrotic specific gene scores (Figure 8E).

[0270] Taken together, these data show that myeloid intrinsic gene score, alone or in combination with lymphoid intrinsic gene score, can serve as a predictive biomarker that predicts the therapeutic efficacy of treatment, including DMARDs.Therefore, for example, the assessment of intrinsic gene score can be used to identify individuals with RA who may benefit from treatment, including DMARDs, as well as to monitor response to treatment, including DMARDs.These results also show the continued presence of myeloid gene expression in individuals who have continued disease activity despite DMARD therapy.

[0271] Example 4. Association between serum gene expression levels and synovial pathology Circulating serum biomarkers hypothesized due to overexpression in the synovial subset of the disease (Dennis et al. Arthritis Research and Therapy. 16(2):R90, 2014) were assessed for their potential to serve as reliable measures of disease activity in the PEAC cohort.

[0272] Serum biomarkers reflect synovial pathophysiology Serum samples from 111 individuals at baseline were assessed for levels of soluble intercellular adhesion molecule 1 (sICAM1), C-X-C motif chemokine 13 (CXCL13), interleukin 8 (IL-8), and matrix metalloproteinase-3 (MMP3) using customized electrochemiluminescence assays incorporating sample diluent blocking reagents to minimize interference from heterophilic antibodies. Serum CXCL13 correlated with comprehensive disease metrics, including DAS28 scores, serological and ultrasound measures of disease activity, and synovial histology (Figures 9A and 9B). Serum MMP-3 also showed a moderate but still significant correlation with acute phase reactants and DAS scores, as well as synovial histology (Figures 9A and 9B). Of note, both CXCL13 and MMP3 were elevated in individuals with lymphoid pathology compared to the other two pathology (Figures 9C and 9D). In contrast, sICAM1 and IL-8 showed moderate and variable correlations with clinical indices such as tender joint scores, acute phase reactants, autoantibody titers, and tender joint scores (Figures 9A and 9B), despite previous reports that they are elevated in RA individuals (Dennis et al. Arthritis Research and Therapy. 16(2):R90, 2014; Cascao et al. Arthritis Research & Therapy. 12(5):R196, 2010).

[0273] In summary, these data demonstrate that elevation of some, but not all, inflammatory proteins in serum in RA correlates with synovitis and clinical disease activity.

[0274] VI. Other Embodiments The foregoing invention has been described in some detail by way of illustration and examples for purposes of clarity of understanding, but these illustrations and examples should not be construed as limiting the scope of the invention. The disclosures of all patent and scientific literature cited herein are expressly incorporated by reference in their entireties.

Claims

1. 1. A method of predicting rheumatoid arthritis (RA) disease progression in an individual having RA, the method comprising determining expression levels of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A in a synovial membrane sample from the individual, wherein an increase in said expression levels compared to a reference expression level identifies the individual as likely to exhibit disease progression of RA; The method of claim 1, wherein said reference expression levels are levels of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A in a synovial sample from a clinically active joint of a non-progressor individual with RA, said non-progressor individual being an individual who has had a synovial biopsy of said joint performed, and said non-progressor individual being identified by a change in Sharp-van der Heijde Radiographic Progression Score (ShSS) of less than 1 one year after synovial biopsy of said joint.

2. 2. The method of claim 1, wherein the RA disease progression is radiographic RA progression.

3. 3. The method of claim 1 or 2, wherein radiographic progression of RA is characterized by an increase in ShSS.

4. 3. A medicament for treating an individual having RA, comprising a therapeutic agent selected from tofacitinib, tocilizumab, and rituximab, wherein the individual has been identified by the method of claim 1 or 2 as one likely to exhibit disease progression of RA.

5. A medicament for treating an individual with RA, wherein the individual has been identified as having increased expression levels of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A in a synovial membrane sample from the individual compared to a reference expression level, the medicament comprising a therapeutic agent selected from tofacitinib, tocilizumab, and rituximab; wherein said reference expression levels are the levels of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A in synovial samples from clinically active joints of non-progressor individuals with RA, said non-progressor individuals being identified by a change in Sharp-van der Heijde radiographic progression score (ShSS) of less than 1 one year after synovial biopsy of the joint.

6. The method of any one of claims 1 to 3, wherein the expression levels of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A are average values ​​of the expression levels of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A.

7. 7. The method of claim 6, wherein the average values ​​of the expression levels of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A are average values ​​of normalized expression levels of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A.

8. The method of any one of claims 1 to 3, wherein the expression levels of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A are the median of the expression levels of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A.

9. 9. The method of claim 8, wherein the median expression levels of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A are median normalized expression levels of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A.

10. The method of claim 7 or 9, wherein the normalized expression levels of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A are the expression levels of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A normalized to a reference gene.

11. 11. The method of claim 10, wherein the reference genes are ACTB, GAPDH, GUSB, HPRT1, PGK1, RPL19, TUBB, TMEM55B, or a combination thereof.

12. The method of any one of claims 1 to 3 and 6 to 11, further comprising determining one or more clinical covariates of the individual.

13. 13. The method of claim 12, wherein the one or more clinical covariates are one or more of Disease Activity Score 28-Erythrocyte Sedimentation Rate (DAS28-ESR), Disease Activity Score 28-C-Reactive Protein (DAS28-CRP), Rheumatoid Factor (RF) titer, disease duration, baseline disease type, and Ultrasound Synovial Thickening (USST) and Ultrasound Power Doppler (USPD) 12 maximum scores.

14. The method of claim 13, wherein the clinical covariate is DAS28-ESR.

15. 14. The method of claim 13, wherein the clinical covariate is RF titer.

16. The method according to any one of claims 1 to 3 and 6 to 15, wherein the expression level is the expression level of a nucleic acid.

17. The method of claim 16, wherein the expression level of the nucleic acid is the expression level of mRNA.

18. 18. The method of claim 17, wherein the mRNA expression levels are determined by direct digital counting of nucleic acids, RNA-seq, RT-qPCR, qPCR, multiplex qPCR or RT-qPCR, microarray analysis, or a combination thereof.

19. The method according to any one of claims 1 to 3 and 6 to 15, wherein the expression level is a protein expression level.

20. 20. The method of claim 19, wherein the expression level of the protein is determined by immunoassay, liquid chromatography-mass spectrometry (LC-MS) technique, nephelometry, aptamer technique, or a combination thereof.

21. The pharmaceutical composition of claim 4 or 5, wherein the expression levels of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A are average values ​​of the expression levels of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A.

22. The pharmaceutical agent of claim 21, wherein the average values ​​of the expression levels of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A are average values ​​of normalized expression levels of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A.

23. The pharmaceutical composition of claim 4 or 5, wherein the expression levels of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A are the median of the expression levels of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A.

24. The pharmaceutical agent of claim 23, wherein the median expression levels of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A are median normalized expression levels of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A.

25. The pharmaceutical of claim 22 or 24, wherein the normalized expression levels of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A are the expression levels of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A normalized to a reference gene.

26. The pharmaceutical composition of claim 25, wherein the reference gene is ACTB, GAPDH, GUSB, HPRT1, PGK1, RPL19, TUBB, TMEM55B, or a combination thereof.

27. The method of any one of claims 4, 5, and 21 to 26, wherein one or more clinical covariates have been determined for the individual.

28. 28. The pharmaceutical of claim 27, wherein the one or more clinical covariates are one or more of Disease Activity Score 28-Erythrocyte Sedimentation Rate (DAS28-ESR), Disease Activity Score 28-C-Reactive Protein (DAS28-CRP), Rheumatoid Factor (RF) titer, disease duration, baseline disease type, and Ultrasound Synovial Thickening (USST) and Ultrasound Power Doppler (USPD) 12 maximum scores.

29. The pharmaceutical composition of claim 28, wherein the clinical covariate is DAS28-ESR.

30. The method of claim 28, wherein the clinical covariate is RF titer.

31. The pharmaceutical agent according to any one of claims 4, 5, and 21 to 30, wherein the expression level is an expression level of a nucleic acid.

32. The pharmaceutical composition of claim 31 , wherein the expression level of the nucleic acid is the expression level of mRNA.

33. The pharmaceutical of claim 32, wherein the mRNA expression level is determined by direct digital counting of nucleic acids, RNA-seq, RT-qPCR, qPCR, multiplex qPCR or RT-qPCR, microarray analysis, or a combination thereof.

34. The pharmaceutical agent according to any one of claims 4, 5, and 21 to 30, wherein the expression level is a protein expression level.

35. The method of claim 34, wherein the expression level of the protein is determined by immunoassay, liquid chromatography-mass spectrometry (LC-MS) technique, nephelometry, aptamer technique, or a combination thereof.

36. The method according to any one of claims 1 to 3 and 6 to 20, wherein the synovial sample is a synovial tissue sample or a synovial fluid sample.

37. The pharmaceutical composition according to any one of claims 4, 5, and 21 to 35, wherein the synovial membrane sample is a synovial tissue sample or a synovial fluid sample.

38. 1. A kit for predicting rheumatoid arthritis (RA) disease progression in an individual having RA, comprising means for determining expression levels of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A in a synovial membrane sample from said individual, wherein an increase in said expression levels compared to a reference expression level identifies said individual as likely to exhibit disease progression of RA; The kit, wherein the reference expression levels are levels of CD180, CSF2, CXCL1, DENND1C, MMP10, SDC1, and UBASH3A in synovial samples from clinically active joints of non-progressor individuals with RA, and non-progressor individuals are identified by a change in Sharp-van der Heijde radiographic progression score (ShSS) of less than 1 one year after synovial biopsy of the joint.

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