Anti-CD30 monoclonal antibodies and chimeric antigen receptors

Abstract

Antibodies having specific binding activity against CD30, fragments thereof, and chimeric proteins comprising same are presented. Advantageously, the contemplated molecules can be used in pharmaceutical compositions for immunotherapy in individuals diagnosed with hematopoietic malignancies, including, inter alia, Hodgkin's lymphoma, CD30-positive B-cell lymphoma, CD30-positive T-cell lymphoma, and CD30-positive NK-cell lymphoma.

Description

[Technical field]

[0001] This application claims priority to the inventors' co-pending U.S. Provisional Patent Application No. 63 / 155,073, filed March 1, 2021, which is incorporated by reference in its entirety.

[0002] Sequence Listing The contents of the ASCII text file of the Sequence Listing, named 102719.0034PRO_REV002_ST25.txt and 11 KB in size, was created on Jan. 20, 2021, and was submitted electronically via EFS-Web along with the present application, and is incorporated by reference in its entirety.

[0003] The field of the invention is target-specific binding molecules, in particular it relates to antibodies and chimeric antigen receptors with binding specificity for CD30, and derivatives thereof. [Background technology]

[0004] The background discussion includes information that may be useful in understanding the invention. No admission is made that any of the information provided herein is prior art or relevant to the claimed invention, or that any publication specifically or implicitly referenced is prior art.

[0005] All publications and patent applications herein are incorporated by reference to the same extent as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference. In the event that the definition or use of a term in the incorporated references is inconsistent or contradicts the definition of that term set forth in this specification, the definition of that term provided in this specification shall apply and the definition of that term in the reference shall not apply.

[0006] CD30 (cluster of differentiation 30, Ki-1, TNFRSF8) belongs to the tumor necrosis factor receptor superfamily and is transiently expressed in humans at relatively low levels on intra- and perifollicular T-cell blasts and B-cell blasts in lymphoid tissues. Notably, CD30 expression is significantly increased in certain hematopoietic malignancies, including anaplastic large cell lymphoma and Hodgkin's lymphoma. Thus, CD30 is a common diagnostic marker for malignant cells in a subset of non-Hodgkin's (NHL) lymphomas, such as Hodgkin's disease (HD) and anaplastic large cell lymphoma (ALCL). More recently, CD30 has emerged as a therapeutic target in the treatment of various hematological disorders, and a number of specific CD30-binding molecules have been developed.

[0007] For example, certain CD30 binding moieties and chimeric antibodies are shown in WO 2020 / 135559 and have been proposed for adoptive T cell therapy in the treatment of cancers or tumors expressing CD30. Similarly, specific chimeric antigen receptors (CARs) with binding activity to CD30 and various uses for these CARs are taught in U.S. Pat. No. 10,815,301, and yet another example of a bispecific antibody that binds to CD30 has been constructed as shown in WO 2020 / 068774.

[0008] In further examples, a method of treating T-cell lymphoma using a specific anti-CD30 antibody-drug conjugate in combination with a second drug is taught in WO 2020 / 072519, and in yet another combination treatment, a specific anti-CD30 antibody was administered with a checkpoint inhibitor to treat Hodgkin's or non-Hodgkin's lymphoma, as disclosed in US Patent Application Publication No. 2019 / 0218293. Still further combination treatments using anti-CD30 antibodies and proteasome inhibitors are disclosed in WO 2018 / 027022 and U.S. Patent No. 7,790,160, and treatment of immunological disorders other than cancer with anti-CD30 antibodies is described in WO 03 / 043583. Although many of the known anti-CD30 binding agents exhibit at least some diagnostic or therapeutic potential, various disadvantages remain. Most importantly, the binding affinity and / or binding specificity of at least some anti-CD30 antibodies or anti-CD30 CARs is less than desirable or has less than desirable therapeutic efficacy.

[0009] Thus, although various systems and methods for anti-CD30 antibodies and CARs are known in the art, all or nearly all of them suffer from several drawbacks. Thus, there remains a need for improved compositions and methods for and uses of anti-CD30 antibodies and CARs. Summary of the Invention [Problem to be solved by the invention]

[0010] The present subject matter is directed to various compositions and methods of CD30-specific therapeutic and diagnostic molecules and their use in the diagnosis and treatment of individuals, particularly cancers in which the cancer cells express or overexpress CD30. [Means for solving the problem]

[0011] In one aspect of the inventive subject matter, the inventors contemplate an isolated antibody or fragment thereof, wherein the antibody or fragment thereof binds to CD30 and comprises a variable heavy chain (VH) domain and a variable light chain (VL) domain, wherein the VH domain is selected from the group consisting of SEQ ID NO:1, SEQ ID NO:3, and SEQ ID NO:5, and the VL domain is selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, and SEQ ID NO:6.

[0012] In one embodiment, the antibody or fragment comprises VH52-2 (SEQ ID NO:1) and VL52-2 (SEQ ID NO:2), optionally linked together by a linker to form an scFv. In another embodiment, the antibody or fragment comprises VH52-5 (SEQ ID NO:3) and VL52-5 (SEQ ID NO:4), optionally linked together by a linker to form an scFv. In a further embodiment, the antibody or fragment comprises VH52-38 (SEQ ID NO:5) and VL52-38 (SEQ ID NO:6), optionally linked together by a linker to form an scFv.

[0013] Most typically, but not necessarily, the antibody is an IgG1 antibody or scFv and / or may further comprise a therapeutic agent (e.g., a chemotherapeutic agent, a radionuclide, or an immune stimulant such as a cytokine analog, a chemokine, or a checkpoint inhibitor). Alternatively or additionally, the antibody or fragment may also comprise a detectable label.

[0014] In other embodiments, the inventors also contemplate chimeric proteins comprising the antibodies or fragments presented herein. For example, the chimeric protein may form a chimeric antigen receptor (CAR), which may have a CD3 zeta (CD3ζ) or Fc receptor epsilon (FcεRIγ) signaling domain, or may have one or more of a CD28 signaling domain, a 4-1BB signaling domain, and a CD3 zeta (CD3ζ) signaling domain. Most typically, the CAR may have a CD8 hinge domain and a CD28 transmembrane domain. As can be easily understood, the CAR will be a recombinant CAR that is expressed and presented on the surface of NK cells or cytotoxic T cells. In other examples, the chimeric protein may form a bispecific fusion protein (e.g., comprising an IgG Fc portion and optionally further comprising at least one of an IL15α receptor portion, an IL15 portion, and an IL15 superagonist portion), or may form a bispecific killer cell engager (BiKE) or a trispecific killer cell engager (TriKe).

[0015] Thus, the inventors also contemplate recombinant nucleic acids encoding the isolated antibodies or fragments, or chimeric proteins presented herein. For example, the nucleic acid may be part of an expression vector or part of a recombinant viral genome, or may be in the form of linear DNA. Alternatively, the recombinant nucleic acid may be RNA.

[0016] Viewed from a different perspective, the inventors also contemplate a pharmaceutical composition comprising a pharma- ceutically acceptable carrier in combination with an isolated antibody or fragment, or chimeric protein provided herein.Similarly, the inventors also contemplate a pharmaceutical composition comprising a pharma- ceutically acceptable carrier in combination with a recombinant nucleic acid presented herein.

[0017] In another aspect of the subject matter of the present invention, the inventors also contemplate a method of treating an individual, in which the pharmaceutical composition provided herein is administered to the individual, typically thereby reducing the immunosuppression in the individual.Most typically, the individual has been treated with a cancer vaccine and / or a checkpoint inhibitor.Therefore, the inventors also contemplate the use of the pharmaceutical composition provided herein in treating hematological cancer in an individual.

[0018] Various objects, features, aspects and advantages of the present subject matter will become apparent from the following detailed description of preferred embodiments, taken in conjunction with the accompanying drawings, in which like numerals represent like elements and in which: [Brief description of the drawings]

[0019] [Figure 1] 1 is a graph displaying exemplary FACS results for binding of anti-CD30 antibodies to cells expressing CD30 and control cells (HL-60) using a commercially available anti-CD30 antibody and an IgG1 antibody in accordance with the present subject matter. [Diagram 2] 1 is a graph displaying exemplary titration results for binding of anti-CD30 antibodies to cells expressing CD30 and control cells (HL-60) using a commercially available anti-CD30 antibody and an IgG1 antibody in accordance with the present subject matter. [Diagram 3] 1 is a graph displaying exemplary results for CAR-specific target cell lysis of CD30-expressing target cells using haNK cells expressing an exemplary anti-CD30 CAR construct and a mock CAR according to the present subject matter. [Figure 4] 1 is a graph displaying exemplary results for CAR-specific target cell lysis of control target cells not expressing CD30 using haNK cells expressing an exemplary anti-CD30 CAR construct and a mock CAR according to the present subject matter. [Diagram 5] FIG. 5 is a graph displaying exemplary results for CD30 expression on target cells of FIGS. 3 and 4. [Figure 6]FIG. 5 is a graph displaying exemplary results for the expression of anti-CD30 CAR and mock CAR in the haNK cells of FIGS. 3 and 4. [Figure 7] Graph displaying exemplary results for CAR-specific target cell lysis (low, high, pooled) of target cells expressing CD30 using haNK cells expressing another exemplary anti-CD30 CAR construct and a mock CAR in accordance with the present subject matter. [Figure 8-1] 8 is a graph displaying exemplary results for CD30 expression on target cells of FIG. 7. [Figure 8-2] Continued from Figure 8-1. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0020] The present inventors have discovered a variety of anti-CD30 antibodies that have high affinity and specificity for binding to CD30. In particularly preferred embodiments, contemplated antibodies have the V H and V L The sequences presented herein are human IgG1 antibodies having the V domain. However, the sequences presented herein can vary, at least to some extent, and thus may have one or more amino acid substitutions, insertions, and / or deletions, as discussed in more detail below. Most typically, but not necessarily, the V domains have the same preceding number (e.g., 52-2). H and V L Domains, or heavy and light chains, may be present in the CD30 binding construct. However, other CD30 binding constructs may include the V H Or V L Domain only or V with non-identical preceding digits H and V L Further, the CD30 binding construct may have at least some of the CDRs (e.g., at least the V domain) as listed below. H domain). 52-2V H Domain amino acid sequence: [ka] 52-2V L Domain amino acid sequence: DIQMTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIYVASNLETGVPSRFSGSGSGTDFTFTISSLQPEDIATYYCQQDADVPLTFGQGTKVEIK (SEQ ID NO: 2) 52-5V H Domain amino acid sequence: [ka] 52-5V L Domain amino acid sequence: DIQMTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIYDASNLETGVPSRFSGSGSGTDFTFTISSLQPEDIATYYCQQDADVPLTFGQGTKVEIK (SEQ ID NO: 4) 52-38V H Domain amino acid sequence: [ka] 52-38V L Domain amino acid sequence: DIQMTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIYDASNLETGVPSRFSGSGSGTDFTFTISSLQPEDIATYYCQQVANVPLTFGQGTKVEIK (SEQ ID NO: 6)

[0021] As can be easily understood, V H and V L The binding specificity of domains is determined by their respective CDR regions, and Table 1 below shows the V H and V L The amino acid sequences for the CDRs in the domains are shown in Table 1. Therefore, it is contemplated that antibodies and fragments thereof that bind to CD30 and contain at least some of the CDRs of SEQ ID NOs: 7 to 24 can be prepared based on the known CDR sequences.

[0022] [Table 1]

[0023] For example, the above CDRs and V H and V L Using the domain information, an IgG1 antibody can be prepared. Most typically, but not necessarily, HC and LC with the same prefix (e.g., 64-6) will be present in the CD30 binding antibody. However, other CD30 binding antibodies may have heavy and light chains with non-identical prefixes.

[0024] Of course, the subject matter of the present invention is not limited to the exact sequences shown above, and one or more of the sequences may contain one or more amino acid changes. Most preferably, these changes will result in a substantial reduction in specificity and / or affinity. Thus, contemplated amino acid changes are typically H and / or V L The amino acid changes will be in the framework regions of the domains and / or in the constant regions of the HC and / or LC. From a different perspective, the amino acid changes are preferably not in the CDR regions. For example, contemplated sequences have 98-99% identity or homology, or 96-98% identity or homology, or 92-96% identity or homology, or 85-92% identity or homology, or 75-85% identity or homology, most typically (but not necessarily) with altered amino acids outside the CDRs. Among other options for amino acid changes, one or more amino acids can be altered to "humanize" a non-human antibody and / or to move or eliminate one or more glycosylation sites.

[0025] It should further be noted that contemplated antibodies explicitly include various forms such as monoclonal antibodies, multispecific antibodies, human antibodies, humanized antibodies, synthetic antibodies, chimeric antibodies, single domain antibodies, single chain Fvs (scFvs), single chain antibodies, disulfide-linked Fvs (sdFvs), BiKe and TriKe as described in more detail below. Of course, it should also be noted that the term antibody explicitly includes all classes of immunoglobulin molecules (e.g., IgG, IgE, IgM, IgD, IgA, and IgY) and the corresponding subclasses (e.g., IgG1, IgG2, IgG3, IgG4, IgA1, IgA2).

[0026] With respect to contemplated antibody fragments, the fragments comprise only the CDRs (typically the V H and V L It should be noted that the term "antibody fragment" includes one or more portions of an antibody that contain at least all of the CDRs of at least one of the CDRs, and optionally framework residues. Thus, an antibody fragment will in most cases exhibit the ability to specifically bind to an antigen (here, an epitope of CD30). Among other fragments, specifically contemplated fragments include Fab', F(ab')2, Fv, scFv, and mutants thereof, naturally occurring variants, and fusion proteins with various non-antibody polypeptides (e.g., toxins, antigen recognition sites for different antigens, enzymes, receptors, receptor ligands, etc.). Viewed from different perspectives, contemplated antibody fragments will have an amino acid sequence of at least 20 contiguous amino acid residues, at least 25 contiguous amino acid residues, at least 40 contiguous amino acid residues, at least 50 contiguous amino acid residues, at least 60 contiguous amino acid residues, at least 70 contiguous amino acid residues, at least 80 contiguous amino acid residues, at least 90 contiguous amino acid residues, at least 100 contiguous amino acid residues, at least 125 contiguous amino acid residues, at least 150 contiguous amino acid residues, at least 175 contiguous amino acid residues, at least 200 contiguous amino acid residues, or at least 250 contiguous amino acid residues.

[0027] In further contemplated aspects, the antibodies or fragments thereof may be used for in vitro or in vivo diagnosis and may be linked to a detectable label in situ. For example, suitable detectable labels include various enzymes, fluorescent materials, luminescent materials, bioluminescent materials, radioactive materials, positron emitting metals, and non-radioactive paramagnetic metal ions. The detectable label may be linked or attached to the antibody either directly or indirectly through an intermediate (e.g., a chemical or biological linker) using techniques known in the art. Additionally or alternatively, contemplated antibodies and fragments thereof may be linked to a solid support, which is particularly useful for immunoassays or purification of CD30 or cells expressing CD30. For example, suitable supports include magnetic beads, glass, cellulose, polyacrylamide, nylon, polystyrene, polyvinyl chloride, and polypropylene.

[0028] Contemplated antibodies and fragments thereof may be linked to or include a therapeutic agent to target the agent to cells expressing CD30. For example, specifically contemplated therapeutic agents include chemotherapeutic agents, radionuclides, and immune stimulants (e.g., cytokines, cytokine analogs, chemokines, or checkpoint inhibitors). There are numerous methods for preparing antibody-drug conjugates, all of which are believed to be suitable for use herein.

[0029] In a particularly preferred embodiment, the contemplated antibody or fragment thereof may be prepared as a chimeric protein in which at least a portion of the antibody is contiguous with a second polypeptide (optionally, preferably via a flexible linker). For example, a suitable chimeric protein may be configured as a chimeric antigen receptor (CAR), which may have an intracellular signaling portion, a transmembrane portion, and an extracellular recognition domain. In such cases, it is generally contemplated that the recognition domain comprises an antibody fragment (e.g., scFv or single domain) and / or that the intracellular signaling domain comprises an activation / ITAM motif. Among other options, first, second, or third generation CARs may be contemplated with various domains known in the art. For example, a suitable CAR would comprise a CD8 hinge portion, a CD28 transmembrane domain, and a CD3 zeta (CD3ζ) or Fc receptor epsilon (FcεRIγ) signaling domain. Alternatively, the signaling domain may also comprise one or more of a CD28 signaling domain, a 4-1BB signaling domain, and a CD3 zeta (CD3ζ) signaling domain. Among other options, such chimeric antigen receptors are preferably expressed in cytotoxic immunocompetent cells, in particular in NK cells and / or T cells.

[0030] Alternatively, contemplated chimeric proteins may be constructed as bispecific fusion proteins, bispecific killer cell engagers (BiKE), or trispecific killer cell engagers (TriKe), particularly when an anti-CD30 antibody or fragment thereof is used to further mediate contact with cells or receptors / ligands. For example, a bispecific fusion protein may comprise an anti-CD30 antibody or portion thereof and a second affinity ligand that selectively binds to a desired target. Such targets may be soluble or cell-associated proteins, and a particularly contemplated target includes PD-L1. Alternatively, contemplated chimeric molecules may be constructed as bispecific polypeptides (e.g., a first scFv linked to a second scFv via a linker) in which one portion comprises an anti-CD30 antibody or portion thereof and the other portion has a binder for a marker specific for immunocompetent cells (e.g., anti-CD3).

[0031] In further contemplated embodiments, the anti-CD30 antibody or portion thereof may bind to an IgG-Fc / IL15Rα / IL15 hybrid (e.g., ALT803). For example, the anti-CD30 antibody fragment may be an scFv portion that binds to one or both arms of the hybrid to form such a TxM (see TxM Technology at URL: Altorbioscience.com). Or, the anti-CD30 antibody fragment may be an scFv portion that binds to one arm of the hybrid, while the other arm of the hybrid may be an scFv portion that binds to PD-L1 (or other immune related ligand).

[0032] As should be understood, nucleic acids encoding contemplated anti-CD30 antibodies are also expressly contemplated herein, and one of skill in the art would be readily able to prepare such nucleic acids (e.g., DNA, RNA) and recombinant entities comprising such nucleic acids. Among other options, suitable recombinant entities include, of course, yeast, bacterial, and viral expression vectors, linear DNA for genome editing or other integration, RNA, and the like, and it should be recognized that the recombinant nucleic acid will include suitable regulatory elements to allow expression of the recombinant construct. Furthermore, it should be noted that the nucleic acid will typically use codon optimization for the host cell that will comprise and express the recombinant nucleic acid.

[0033] As will be readily appreciated, the use of anti-CD30 antibodies, fragments thereof, or chimeric proteins containing anti-CD30 antibodies or fragments thereof is particularly advantageous when CD30-positive tumor cells are targeted using anti-CD30 antibodies on cytotoxic immune cells and / or anti-CD30 CARs. Furthermore, when cancer cells express and display CD30, the cells may present an additional therapeutic target (e.g., via targeting with a chimeric molecule having a CD30-binding portion and an immune stimulatory portion (e.g., ALT-803)).

[0034] In view of these findings, the inventors also contemplate the use of various recombinant CD30 binding molecules, such as antibodies and fragments thereof, as well as cells expressing anti-CD30 CAR molecules and pharmaceutical compositions comprising them. Most typically, such recombinant proteins can be soluble forms of antibodies and fragments thereof, soluble chimeric molecules comprising a CD30 binding moiety, or membrane-bound molecules such as CARs comprising a CD30 binding moiety. For example, recombinant CD30-binding CARs can be expressed in cytotoxic cells, such as T cells, natural killer cells, or NKT cells.

[0035] It is contemplated that such prepared or produced pharmaceutical compositions can be administered to a patient bearing a tumor to enhance the efficacy of immunotherapy and treat the tumor (e.g., modulate (e.g., reduce, eliminate, etc.) tumor-induced immunosuppression, reduce tumor size, etc.). In some embodiments, the pharmaceutical compositions and / or tumor vaccines may be administered via systemic injection, including subcutaneous, subdermal, or intravenous injection. In other embodiments, where systemic injection is not effective (e.g., in the case of brain tumors, etc.) or where a more localized treatment is desired, it is contemplated that the recombinant immunoglobulin protein complex and / or pharmaceutical composition may be administered via intratumoral injection. As used herein, the term "administering" refers to both direct and indirect administration of the compounds and compositions contemplated herein, with direct administration typically being performed by a medical professional (e.g., a doctor, nurse, etc.), while indirect administration typically involves providing the compounds and compositions to a medical professional for direct administration or making the compounds and compositions available to a medical professional for direct administration.

[0036] With regard to the dose and schedule of administration, the dose and / or schedule may vary depending on the type of protein, protein complex, or type of pharmaceutical composition (e.g., virus, bacteria, yeast, etc. combined with the recombinant protein complex), the type and prognosis of the disease (e.g., tumor type, size, location), and the health status of the patient (including, e.g., age, sex, etc.). Although the dose and schedule may vary, the dose and schedule may be selected and adjusted such that the formulation does not cause any significant adverse effects on normal cells of the host, while still being sufficient to reduce immune suppression by reducing T cell differentiation and / or activation in the tumor microenvironment. Thus, in a preferred embodiment, the optimal or desirable conditions for administering the formulation may be determined based on a predetermined threshold. For example, the predetermined threshold may be the concentration of activated T cells in the tumor microenvironment, or T cell-released cytokines (e.g., IL-2, IL-12, IFN-γ, IL-12, IL-23, IL-1b, IL-6, or TGF-β) at a given location or systemically. Thus, administration conditions are typically adjusted to increase one or more of these cytokines in the tumor microenvironment by at least 20%, at least 30%, at least 50%, at least 60%, at least 70%, for at least 24 hours, 48 ​​hours, 72 hours, 7 days, etc. Additionally, the compounds and compositions provided herein may be administered in combination (concurrently or sequentially) with NK cells. For example, suitable NK cells include autologous NK cells, as well as NK92 cells and their derivatives (e.g., aNK cells, haNK cells, taNK cells, all commercially available from NantKwest, 9920 Jefferson Blvd. Culver City, CA 90232). EXAMPLES

[0037] As mentioned above, V H and V L Using the domains, the inventors have prepared a variety of CD30 binding constructs, and exemplary results for scFv and IgG1 constructs are shown below. For example, Table 2 below shows the V H and V LExemplary results are shown for the determination of dissociation constants for scFv and IgG1 constructs using the K D The determination of is done by SPR and the average value is multiplied by 10 -9 The measurements are indicated with M. Measurements were performed at 25° C. and pH 7.4, with scFv or IgG1 captured on the chip surface and CD30 was the analyte.

[0038] [Table 2]

[0039] To demonstrate in vitro binding of anti-CD30 constructs to cells expressing CD30, we constructed and used anti-CD30 IgG1(52-5) to expose cells expressing CD30 (here, Jurkat lymphoma cells) to a commercially available anti-CD30 antibody along with anti-CD30-IgG1 antibody. HL-60 cells, which do not express CD30, served as a negative control. As can be easily seen from the flow cytometry results in FIG. 1, anti-CD30 IgG1(52-5) bound strongly and specifically to Jurkat cells and showed virtually no binding to HL-60 cells. Furthermore, the binding of anti-CD30-IgG1(52-5) was stronger than the commercially available anti-CD30 antibody. When anti-CD30-IgG1(52-38) was used to quantify binding to Jurkat cells, a dose-response curve was obtained, as can be seen in FIG. 2, while the IgG control did not give significant binding.

[0040] In a series of further experiments, the inventors have L and V HAn anti-CD30 CAR was constructed that includes an scFv portion having a CD8 hinge portion, a CD28 transmembrane portion, and an Fc receptor epsilon (FcεRIγ) signaling domain (linked by a flexible linker portion). FIG. 3 is a graph displaying exemplary results for CAR-specific target cell lysis of CD30-expressing target cells using haNK cells expressing the above-mentioned anti-CD30 CAR and mock CAR. FIG. 4 is a graph displaying corresponding exemplary results for CAR-specific target cell lysis of control target cells that do not express CD30. FIG. 5 is a graph displaying exemplary results for CD30 expression in the target cells of FIGS. 3 and 4, and FIG. 6 is a graph displaying exemplary results for anti-CD30 CAR and mock CAR expression in haNK cells of FIGS. 3 and 4.

[0041] Notably, CAR-mediated cytotoxicity was strictly correlated with the amount of CD30 present on the target cells, as can be seen in Figure 7. Here, CAR-specific target cell lysis of CD30-expressing target cells is shown for cells with low and high expression levels of CD30, as well as for pools of these cells. Figure 8 exemplarily displays the results for CD30 expression on target cells in Figure 7.

[0042] In some embodiments, numbers expressing properties such as amounts and concentrations of ingredients, reaction conditions, and the like, used to describe and claim certain embodiments of the present invention should be understood to be modified in some cases with the term "about". Thus, in some embodiments, the numerical parameters set forth in the specification and appended claims are approximations that may vary depending on the desired properties sought to be obtained by a particular embodiment. The recitation of ranges and values ​​herein is intended only to serve as a shorthand for individually referring to each individual value falling within the range. Unless otherwise stated herein, each individual value is incorporated herein as if it were individually recited herein.

[0043] As used herein, the term "administering" a pharmaceutical composition or drug refers to both direct and indirect administration of the pharmaceutical composition or drug, where direct administration of the pharmaceutical composition or drug is typically performed by a medical practitioner (e.g., a doctor, a nurse, etc.), and indirect administration includes providing the pharmaceutical composition or drug to a medical practitioner for direct administration (e.g., via injection, infusion, oral delivery, topical delivery, etc.) or making the pharmaceutical composition or drug available to a medical practitioner for direct administration. It should be further noted that the term "prognosing" or "predicting" a condition, a state susceptible to developing a disease, or a response to an intended treatment is meant to cover the act or prediction of the condition, susceptibility to development, and / or response, including the rate of progression, improvement, and / or duration of the condition in a subject (but not covering treatment or diagnosis).

[0044] All methods described herein can be performed in any suitable order unless otherwise specified herein or clearly contradicted by context. Any and all examples provided for certain embodiments herein, or the use of exemplary language (e.g., "etc.") are intended merely to facilitate a better understanding of the invention and do not pose a limitation on the scope of the invention as otherwise claimed. No language in the specification should be construed as indicating any non-claimed element essential to the practice of the invention.

[0045] As used throughout this description and the claims that follow, the meanings of "a," "an," and "the" include plural referents unless the content clearly dictates otherwise. Also, as used in this description, the meaning of "in" includes "in" and "on," unless the content clearly dictates otherwise. Also, as used herein, and unless the content clearly dictates otherwise, the term "coupled to" is intended to include both direct coupling (where the two elements that are coupled to each other are in contact with each other) and indirect coupling (where at least one additional element is located between the two elements). Thus, "coupled to" and "coupled with" are used synonymously.

[0046] It will be apparent to those skilled in the art that many other modifications besides those already described are possible without departing from the inventive concept herein. Thus, the subject matter of the present invention should not be limited except as by the appended claims. Moreover, in interpreting both the specification and the claims, all terms should be interpreted as broadly as possible consistent with the context. In particular, the terms "comprises" and "comprising" should be interpreted as referring to elements, components, or steps in a non-exclusive manner, indicating that the referred element, component, or step may be present or utilized, or may be combined with other elements, components, or steps not expressly mentioned. When a claim in the specification refers to at least one of several selected from the group consisting of A, B, C... and N, the document should be interpreted as requiring only one element from the group, and not as requiring A plus N, or B plus N, etc. The present invention includes, for example, the following embodiments: [Embodiment 1] An isolated antibody or fragment thereof, wherein the antibody or fragment thereof binds to CD30, Variable heavy chain (V H ) domain and the variable light chain (V L ) domain, The above V H the domain is selected from the group consisting of SEQ ID NO:1, SEQ ID NO:3, and SEQ ID NO:5; The above V L An antibody or fragment thereof, wherein the domain is selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, and SEQ ID NO:6. [Embodiment 2] Optionally, VH are linked together by a linker to form an scFv. 52-2 (SEQ ID NO: 1) and VL 52-2 2. The antibody or fragment of embodiment 1, comprising:

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[0039]

[0047] [ 52-5 (SEQ ID NO: 3) and VL 52-5 2. The antibody or fragment of embodiment 1, comprising:

[0036] [Embodiment 4] Optionally, VH are linked together by a linker to form an scFv. 52-38 (SEQ ID NO: 5) and VL 52-38 2. The antibody or fragment of embodiment 1, comprising: (SEQ ID NO: 6). [Embodiment 5] The antibody is IgG 1 The antibody or fragment of embodiment 1, which is an antibody or scFv. [Embodiment 6] The antibody or fragment described in any one of embodiments 1 to 5, further comprising a therapeutic agent. [Embodiment 7] The antibody or fragment described in embodiment 6, wherein the therapeutic agent is a chemotherapeutic agent, a radionuclide, or an immunostimulant. [Embodiment 8] The antibody or fragment described in embodiment 7, wherein the immunostimulatory agent is a cytokine, a cytokine analog, a chemokine, or a checkpoint inhibitor. [Embodiment 9] The antibody or fragment described in any one of embodiments 1 to 8, further comprising a detectable label. [Embodiment 10] A chimeric protein comprising the antibody or fragment described in embodiment 1. [Embodiment 11] The chimeric protein of embodiment 10, wherein the chimeric protein is a chimeric antigen receptor (CAR). [Embodiment 12] The chimeric protein of embodiment 11, wherein the CAR has a CD3 zeta (CD3ζ) or Fc receptor epsilon (FcεRIγ) signaling domain. [Embodiment 13] The chimeric protein of embodiment 11, wherein the CAR has at least one of a CD28 signaling domain, a 4-1BB signaling domain, and a CD3 zeta (CD3ζ) signaling domain. [Embodiment 14] The chimeric protein of embodiment 11, wherein the CAR has a CD8 hinge domain and a CD28 transmembrane domain. [Embodiment 15] The chimeric protein of embodiment 11, wherein the CAR is a recombinant CAR that is expressed on the surface of and binds to NK cells or cytotoxic T cells. [Embodiment 16] The chimeric protein described in embodiment 10, configured as a bispecific fusion protein. [Embodiment 17] The chimeric protein of embodiment 16, wherein the bispecific fusion protein comprises an IgG Fc portion and optionally further comprises at least one of an IL15α receptor portion, an IL15 portion, and an IL15 superagonist portion. [Embodiment 18] The chimeric protein described in embodiment 16, configured as a bispecific killer cell engager (BiKE) or a trispecific killer cell engager (TriKe). [Embodiment 19] A recombinant nucleic acid encoding the isolated antibody or fragment described in any one of embodiments 1 to 9, or the chimeric protein described in any one of embodiments 10 to 18. [Embodiment 20] The recombinant nucleic acid described in embodiment 19, wherein the nucleic acid is in an expression vector or a recombinant viral genome, or in the form of linear DNA. [Embodiment 21] The recombinant nucleic acid described in embodiment 19, wherein the nucleic acid is RNA. [Embodiment 22] A pharmaceutical composition comprising an isolated antibody or fragment described in any one of embodiments 1 to 9, or a chimeric protein described in any one of embodiments 10 to 18, in combination with a pharma- ceutically acceptable carrier. [Embodiment 23] A pharmaceutical composition comprising a pharma- ceutically acceptable carrier combined with the recombinant nucleic acid described in any one of embodiments 19 to 21. [Embodiment 24] A method for treating an individual, comprising administering a pharmaceutical composition described in any one of embodiments 22 to 23. [Embodiment 25] The method of embodiment 24, wherein the pharmaceutical composition is administered to the individual, thereby reducing immunosuppression in the individual. [Embodiment 26] The method of embodiment 24, wherein the individual is being treated with a cancer vaccine and / or a checkpoint inhibitor. [Embodiment 27] Use of a pharmaceutical composition described in any one of embodiments 22 to 23 in the treatment of hematological cancer in an individual.

Claims

1. An isolated antibody or fragment thereof, wherein the antibody or fragment thereof binds to CD30; Variable heavy chains (VH) consisting of SEQ ID NO:1 and SEQ ID NO:2, SEQ ID NO:3 and SEQ ID NO:4, SEQ ID NO:3 and SEQ ID NO:2, or SEQ ID NO:1 and SEQ ID NO:4, respectively. Ｈ ) domain and the variable light chain (V Ｌ ) domain.

2. VH, optionally linked together by a linker to form a scFv ５２－２ (SEQ ID NO: 1) and VL ５２－２ The antibody or fragment of claim 1 comprising: (SEQ ID NO:2).

3. VH, optionally linked together by a linker to form a scFv ５２－５ (SEQ ID NO: 3) and VL ５２－５ The antibody or fragment of claim 1 comprising: (SEQ ID NO: 4).

4. VH, optionally linked together by a linker to form a scFv ５２－２ (SEQ ID NO: 1) and VL ５２－５ The antibody or fragment of claim 1 comprising: (SEQ ID NO: 4).

5. VH, optionally linked together by a linker to form a scFv ５２－５ (SEQ ID NO: 3) and VL ５２－２ The antibody or fragment of claim 1 comprising: (SEQ ID NO:2).

6. The antibody or fragment is an IgG １ The antibody or fragment of claim 1 which is an antibody or scFv.

7. The antibody or fragment of any one of claims 1 to 6, wherein the antibody or fragment is conjugated to a detectable label.

8. A chimeric protein comprising the antibody or fragment of claim 1.

9. The chimeric protein of claim 8, wherein the chimeric protein is a chimeric antigen receptor (CAR).

10. The chimeric protein of claim 9, wherein the CAR has a CD3 zeta (CD3ζ) or Fc receptor epsilon (FcεRIγ) signaling domain.

11. The chimeric protein of claim 9, wherein the CAR comprises at least one of a CD28 signaling domain, a 4-1BB signaling domain, and a CD3 zeta (CD3ζ) signaling domain.

12. The chimeric protein of claim 9, wherein the CAR has a CD8 hinge domain and a CD28 transmembrane domain.

13. The chimeric protein of claim 9, wherein the CAR is a recombinant CAR expressed on the surface of a NK cell or a cytotoxic T cell.

14. The chimeric protein of claim 8 configured as a bispecific fusion protein.

15. The chimeric protein of claim 14, wherein the bispecific fusion protein comprises an IgG Fc portion and optionally further comprises at least one of an IL15α receptor portion, an IL15 portion, and an IL15 superagonist portion.

16. 15. The chimeric protein of claim 14 configured as a bispecific killer cell engager (BiKE) or a trispecific killer cell engager (TriKe).

17. A recombinant nucleic acid encoding the isolated antibody or fragment of any one of claims 1 to 7, or the chimeric protein of any one of claims 8 to 16.

18. 18. The recombinant nucleic acid of claim 17, wherein the nucleic acid is in an expression vector or a recombinant viral genome, or in the form of linear DNA.

19. The recombinant nucleic acid of claim 17 , wherein the nucleic acid is RNA.

20. A pharmaceutical composition comprising the isolated antibody or fragment of any one of claims 1 to 7, or the chimeric protein of any one of claims 8 to 16, and a pharma- ceutically acceptable carrier.

21. A pharmaceutical composition comprising the recombinant nucleic acid of any one of claims 17 to 19 and a pharma- ceutically acceptable carrier.

22. A pharmaceutical composition for use in the treatment of an individual, comprising a pharmaceutical composition according to any one of claims 20 to 21.

23. 23. The pharmaceutical composition of claim 22, formulated for administration to said individual, thereby reducing immunosuppression in said individual.

24. 23. The pharmaceutical composition of claim 22, wherein the individual is being treated with a cancer vaccine and / or a checkpoint inhibitor.

25. 21. The pharmaceutical composition of claim 20, further comprising a therapeutic agent.

26. 26. The pharmaceutical composition of claim 25, wherein the therapeutic agent is a chemotherapeutic agent, a radionuclide, or an immunostimulant.

27. 27. The pharmaceutical composition of claim 26, wherein the immunostimulatory agent is a cytokine, a cytokine analog, a chemokine, or a checkpoint inhibitor.

28. 21. Use of the pharmaceutical composition of claim 20 in the manufacture of a medicament for the treatment of hematological cancer in an individual.

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