Anti-pollution agent and composition for external use on skin

Abstract

To provide an antipollution agent and an antipollution topical skin composition having an excellent preventative or suppressive effect on skin inflammation due to microparticulate atmospheric pollutants.SOLUTION: The present invention provides an antipollution agent in which a plant-derived polyamide-containing extract prevents or suppresses skin inflammation due to atmospheric pollutants.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to an antipollution agent that alleviates damage to the skin caused by air pollutants, particularly particulate air pollutants, and to an external composition for skin containing said antipollution agent. [Background technology]

[0002] The skin is the largest and outermost tissue, and is exposed to external stimuli such as ultraviolet rays, microorganisms, viruses, and air pollutants. Ultraviolet rays have long been studied as a cause of skin aging by damaging the skin. On the other hand, in recent years, there have been an increasing number of reports on the possibility that adhesion of particulate air pollutants to the skin may be involved in aging (Non-Patent Document 1). In addition, it has been reported that benzopyrene contained in tobacco activates the aryl hydrocarbon receptor present in epidermal cells, promoting the induction of the drug metabolizing enzyme cytochrome P450 (CYP1), producing active oxygen, inducing the production of interleukin 8, and inducing an inflammatory response (Non-Patent Document 2).

[0003] In order to protect the skin from these external stimuli, it is possible to prevent substances that can cause damage, such as particulate air pollutants, from adhering to or penetrating the skin and / or to mitigate the impact of adhering irritating substances on the inside of the skin.

[0004] As described above, as a measure to protect the skin, there have been proposals for agents that form a film on the skin to prevent particulate air pollutants from adhering to the skin or to allow them to be easily washed off if they do adhere. However, these agents have an unpleasant feel when used because they cover the skin with a film, and it has been unclear whether they are effective against all irritating substances.

[0005] It has been studied that particulate air pollutants cause oxidative stress in cells by adhering to and penetrating the skin, leading to inflammatory reactions, cell metabolism, and genetic damage. However, the mechanisms of cell damage are extremely diverse, including oxidation, inflammation, and abnormalities in cell metabolism and gene expression, and many aspects remain unknown. Perhaps for this reason, there are currently not many materials on the market that have anti-pollution effects.

[0006] For example, it has been found that an extract of the Hippophae genus of the Elaeagnaceae family suppresses an increase in PGE2 caused by atmospheric aerosol particles such as particulate air pollutants, thereby suppressing skin inflammation caused by atmospheric aerosol particles (Patent Document 1).Furthermore, focusing on the mechanism by which pollutants that have penetrated into the skin cause skin inflammation in epidermal cells, it has been found to be useful in alleviating cell damage by zinc salts and / or zinc complexes of amino acids, and suppressing CYP1 over-induction, COX2 over-induction, and IL8 over-induction (Patent Document 2).

[0007] In addition to the above circumstances, there is a need for the development of new anti-pollution agents from the standpoints of diversifying formulations, expanding options for formulation combinations, achieving synergistic effects when using anti-pollution agents against particulate air pollutants that have different mechanisms, and providing safer and more effective anti-pollution agents.

[0008] Polyamines are a general term for aliphatic hydrocarbons with two or more primary amino groups, and are natural products that are ubiquitous in living organisms. More than 20 types of polyamines have been found. Representative polyamines include putrescine, spermidine, and spermine. The main physiological effects of polyamines are known to be (1) stabilization and structural change of nucleic acids through interaction with nucleic acids, (2) promotion of various nucleic acid synthesis systems, (3) activation of protein synthesis systems, (4) stabilization of cell membranes and enhancement of membrane permeability of substances, (5) elimination of active oxygen, and (6) promotion of cell proliferation. However, the effect of polyamines in preventing or suppressing skin inflammation caused by particulate air pollutants has not been confirmed until now. [Prior art documents] [Patent documents]

[0009] [Patent Document 1] JP 2016-216366 A [Patent Document 2] JP 2016-88929 A [Non-patent literature]

[0010] [Non-Patent Document 1] J Eur Acad Dermatol Venereol. 2015 Dec;29(12):2326-32 [Non-Patent Document 2] J Dermatol Sci. 2011 Apr;62(1):42-9 Summary of the Invention

[0011] The present invention provides an antipollution agent which is highly effective in preventing or suppressing skin inflammation and the like caused by particulate air pollutants, and a composition for external use on the skin which uses said antipollution agent. [Means for solving the problem]

[0012] The present inventors have discovered that a plant-derived polyamine-containing extract has an anti-pollution effect of alleviating skin inflammation and the like caused by air pollutants, and have thus completed the present invention.

[0013] That is, the outline of the present invention is as follows. Item 1. An anti-pollution agent containing a plant-derived polyamine-containing extract as an active ingredient. Item 2. An anti-pollution agent according to Item 1, wherein the plant-derived polyamine-containing extract is an extract derived from at least one material selected from the group consisting of soybean seeds, soybean germs, soybean embryos, soybean sprouts, wheat seeds, wheat germs, wheat embryos, wheat sprouts, soy milk and soybean lees. Item 3. An anti-pollution agent according to Item 1 or 2, wherein the plant-derived polyamine-containing extract is an extract derived from soybean germ. Item 4. The antipollution agent according to any one of Items 1 to 3, which contains, as a polyamine, at least one compound selected from the group consisting of putrescine, spermidine and spermine. Item 5. The antipollution agent according to any one of Items 1 to 4, which has an effect of suppressing the expression of at least one gene selected from the group consisting of interleukin-1α (IL1α), interleukin-8 (IL8) and cytochrome P450 (CYP1). Item 6. A composition for external use on skin, comprising the antipollution agent according to any one of items 1 to 5. Effect of the Invention

[0014] Use of the plant-derived polyamine-containing extract of the present invention is expected to prevent or inhibit skin inflammation caused by particulate air pollutants, and an external skin preparation having such effects can be provided. [Brief description of the drawings]

[0015] [Figure 1] FIG. 1 shows the results of Example 1 in which keratinocytes were pretreated with a soybean extract and then treated with urban air dust (environmental standard substance) / CRM No. 28) 5 hours later, and the expression level of IL1α after 2 hours. [Diagram 2] FIG. 1 shows the results of Example 1 in which keratinocytes were pretreated with a soybean extract and then treated with urban air dust (environmental standard substance) / CRM No. 28) 5 hours later, and the expression level of IL8 after 2 hours. [Diagram 3] FIG. 1 shows the results of keratinocytes treated with a soybean extract in advance, and then treated with urban air dust (environmental standard substance) / CRM No. 28) 5 hours later, in Example 1, showing the expression level of CYP1 2 hours later.

[0016] In the present invention, air pollutants refer to harmful substances or groups of harmful substances present in the air. Specifically, they include soot, dust, automobile exhaust gas, specific substances, harmful air pollutants, and volatile organic compounds (VOCs), which are subject to regulation under the Air Pollution Control Act (Japan Environmental Restoration and Conservation Agency website; https: / / www.erca.go.jp / yobou / taiki / taisaku / 01_01.html), but are not limited thereto. More specifically, they include suspended particulate matter consisting of multiple types of harmful substances such as PM10 and PM2.5, and the anti-pollution agent of the present invention and the skin topical composition containing the anti-pollution agent can particularly prevent or suppress skin inflammation caused by PM2.5. In the present invention, anti-pollution refers to the effect of preventing or suppressing skin inflammation caused by air pollutants. Specifically, the effect of suppressing gene expression of IL1α, IL8, or CYP1 can be used as a criterion for judgment, but is not particularly limited thereto.

[0017] In the present invention, the "plant extract" is a substance obtained from a plant and / or a processed plant product. In the present invention, the term "extract containing polyamine derived from a plant" refers to a plant extract containing (natural) polyamines obtained from a plant and / or a processed plant product, a plant extract containing three types of (natural) putrescine, spermidine, and spermine obtained from a plant and / or a processed plant product, and a plant extract containing three types of (natural) putrescine, spermidine, and spermine simultaneously obtained from a plant and / or a processed plant product. In the present invention, the "extract containing polyamine derived from a plant" may also be referred to as "a plant extract containing polyamines and / or a plant extract containing polyamines as an active ingredient". The concentration of polyamines contained in the extract containing polyamine derived from a plant is usually 0.00001 to 100 mM, preferably 0.00005 to 75 mM, and more preferably 0.0001 to 50 mM, in terms of moles / liter. In terms of weight percent, the concentration is usually 0.0001 to 100%, preferably 0.001 to 75%, and more preferably 0.01 to 50%.

[0018] Since the plant-derived polyamine-containing extract contains natural active ingredients other than polyamines such as putrescine, spermidine, and spermine, it is presumed that the plant-derived polyamine-containing extract has a better anti-pollution effect than a composition containing chemically synthesized putrescine, spermidine, and spermine individually. Examples of the natural active ingredients include saccharides such as monosaccharides and oligosaccharides, peptides, and proteins. In addition, since the plant-derived polyamine-containing extract is an aqueous solution, the production conditions are milder than those of individual polyamine compounds, and it is expected to have a high merit in terms of safety.

[0019] In the present invention, polyamine is a general term for aliphatic hydrocarbons having two or more primary amino groups, and is a natural product that is ubiquitously present in the body, and more than 20 types of polyamines have been found. For example, 1,3-diaminopropane, putrescine, cadaverine, caldine, spermidine, homospermidine, aminopropylcadaverine, thermine, spermine, thermospermine, canavalmine, aminopentylnorspermidine, N,N-bis(aminopropyl)cadaverine, homospermine, caldopentamine, homocaldopentamine, caldohexamine, homocaldohexamine, etc. Representative polyamines include putrescine, spermidine, spermine, etc.

[0020] "Putrescine" as referred to in this invention is one of the representative polyamines, a common natural product ubiquitously present in living organisms, and an aliphatic hydrocarbon compound with two primary amino groups. "Spermidine" is one of the representative polyamines, a common natural product ubiquitously present in living organisms, and an aliphatic hydrocarbon compound with three primary amino groups. "Spermine" is one of the representative polyamines, a common natural product ubiquitously present in living organisms, and an aliphatic hydrocarbon compound with four primary amino groups.

[0021] Plants from which plant extracts containing polyamines and / or plant extracts containing polyamines as an active ingredient can be obtained include, but are not limited to, dicotyledonous plants, monocotyledonous plants, herbaceous plants, woody plants, Cucurbitaceae plants, Solanaceae plants, Gramineae plants, Cruciferae plants, Leguminous plants, Malvaceae plants, Asteraceae plants, Chenopodiaceae plants, Leguminous plants, and extracts of such plants. For example, sweet potato, tomato, cucumber, pumpkin, melon, watermelon, tobacco, Arabidopsis, bell pepper, eggplant, bean, taro, spinach, carrot, strawberry, potato, rice, corn, alfalfa, wheat, barley, soybean, rapeseed, sorghum, eucalyptus, poplar, kenaf, eucommia, sugarcane, sugar beet, cassava, sago palm, pigweed, lily, orchid, carnation, rose, chrysanthemum, petunia, torenia, goldfish Examples of effective ingredients include grass, cyclamen, baby's breath, geranium, sunflower, turf grass, cotton, enoki mushroom, shimeji mushroom, matsutake mushroom, shiitake mushroom, mushrooms, ginseng, agaricus, turmeric, ginseng, citrus fruits, green tea, black tea, oolong tea, banana, kiwi, natto, soy milk, soybean extract, wheat extract, germ extract, germ extract, fruit juice, soybean lees, rice germ, wheat germ, barley germ, soybean germ, corn germ, milo germ, and sunflower germ.

[0022] Preferably, monocotyledonous or dicotyledonous plants are used, more preferably, gramineous or legumes are used, and particularly preferred plants or processed products thereof include corn, mushrooms, soybeans, wheat, natto, soy milk, okara, wheat germ, soybean germ, corn germ, soybean extract, wheat extract, germ extract, and germ extract. More preferred plants include soybean seeds, soybean germ, soybean embryos, soybean sprouts, wheat seeds, wheat germs, wheat embryos, wheat sprouts, soy milk, and okara.

[0023] The plant tissue from which the plant extract containing polyamine and / or the plant extract containing polyamine as an active ingredient is obtained is not particularly limited. Preferably, it is in the form of a seed or in the process of growth. The plant in the process of growth can be obtained from the whole or partial tissue. The parts that can be obtained are not particularly limited, but include the whole tree, flowers, buds, ovaries, fruits, leaves, cotyledons, stems, buds, roots, seeds, dried seeds, embryos, germs, roots, etc. Preferably, it is fruits, leaves, stems, buds, seeds, dried seeds, germs, embryos, etc., and more preferably, it is seeds, dried seeds, germs, embryos, etc.

[0024] The plant extract containing polyamine and / or the plant from which the plant extract containing polyamine as an active ingredient is obtained may be a processed plant product. The processing method involves recovering the extract from the plant by impregnation, distillation, squeezing, ultrasonication, supercritical fluid method, subcritical fluid method, etc. under low temperature, room temperature, or heated conditions using water, an organic solvent, or a mixture of water and an organic solvent. Furthermore, processed products obtained by processing the plant or the extract recovered from the plant, such as fermentation, are also included. Examples include plant extracts, soy milk, bean curd refuse, wheat flour, fermented extracts, natto, etc.

[0025] A method for obtaining a plant extract containing polyamines and / or a plant extract containing polyamines as an active ingredient is to add an acid solution to a plant and / or a processed plant product so that the plant and / or the processed plant product are under acidic conditions. The acidic conditions refer to conditions where the pH is 6 or less. By making the pH acidic during extraction, an effect of efficient and stable recovery of a polyamine composition from plant tissue can be obtained. This effect can be uniformly obtained if the pH is 6 or less, but preferably the pH is 4 or less, and particularly preferably the pH is 2 or less. The lower limit is not particularly limited because it may be the pH of the original acid solution used, but is preferably pH 0 to 2.

[0026] By obtaining plant extracts under acidic conditions (acid aqueous solution), the recovery rate of polyamines is higher than that of plant extracts obtained using organic solvents such as ethanol or methanol. In particular, compound polyamines, which are poorly soluble in water, can be solubilized in water under acidic conditions, and the content of free polyamines, which have low extraction efficiency in organic solvents, is also improved. Polyamines show excellent stability under acidic conditions, and the stability of polyamines and other active ingredients contained in the plant extract is improved. Furthermore, by obtaining plant extracts under acidic conditions (acid aqueous solution), natural active ingredients other than polyamines are simultaneously recovered. For example, natural active ingredients include monosaccharides, oligosaccharides, and other sugars, peptides, proteins, etc. The anti-pollution effect is further enhanced by the simultaneous inclusion of polyamines and natural active ingredients other than polyamines. In addition, since the recovered plant extract is an aqueous solution, it is expected to have a high merit in terms of safety compared to organic solvents.

[0027] Examples of the acid solution to be added to create an acidic condition include mineral acids such as hydrochloric acid, sulfuric acid, and phosphoric acid; organic acids such as acetic acid, propionic acid, butyric acid, oxalic acid, malonic acid, succinic acid, fumaric acid, maleic acid, malic acid, lactic acid, tartaric acid, citric acid, and benzoic acid; and acidic water. Examples of the acid solution to be added to create an acidic condition include inorganic acids and organic acids such as 0.01N to 6N hydrochloric acid, sulfuric acid, nitric acid, acetic acid, phosphoric acid, trichloroacetic acid, sulfosalicylic acid, formic acid, citric acid, and lactic acid, and 0.1 to 10% perchloric acid.

[0028] By adding an acid solution to a plant and / or a processed plant product to create an acidic condition, or adding an acid solution to create an acidic condition and then adding a polyphenol adsorbent, followed by crushing, grinding, and mixing, the recovery amount of a plant extract containing polyamines and / or a plant extract containing polyamines as an active ingredient can be increased. In particular, in the case of plant tissue, since it has a cell wall, it is desirable to damage the cell wall. In the case of processed plant products and plant extracts, since they do not contain a cell wall, there is no need to crush or grind in a way that damages the cell wall. For example, a mixer, a blender, a homogenizer, a mortar, an ultrasonic crusher, etc. can be used as a method for crushing or grinding.

[0029] Polyamines contained in plants and / or plant products are thoroughly extracted into an acid solution (liquid fraction), and the liquid fraction is then separated from residues and precipitates by centrifugation or filtration. The recovered liquid fraction contains a large amount of polyamines, and a "plant extract containing polyamines and / or a plant extract containing polyamines as an active ingredient" can be obtained.

[0030] The plant extract containing polyamine and / or the plant extract containing polyamine as an active ingredient may be used as an anti-pollution agent as it is, but it is preferable to use it by blending it as a skin external preparation. The concentration of polyamine to be blended is determined depending on the degree of absorption, the degree of action, the product form, the frequency of use, etc., and is not particularly limited, but is usually 0.00001 to 100 mM, preferably 0.00005 to 75 mM, more preferably 0.0001 to 50 mM.

[0031] The anti-pollution agent and anti-pollution skin topical composition of the present invention contain a plant-derived polyamine-containing extract as an active ingredient. In the present invention, anti-pollution refers to the effect of alleviating skin damage caused by air pollutants. More specifically, for example, skin damage can be alleviated by preventing or suppressing cellular inflammation caused by air pollutants adhering to and penetrating the skin.

[0032] The mechanism of action of the anti-pollution effect of the present invention is presumably that the plant-derived polyamine-containing extract, which is the active ingredient of the present invention, acts inside and outside the epidermal cells of the skin, alleviating the inflammatory action of cells that occurs when air pollutants adhere to or enter the skin.

[0033] More specifically, the anti-pollution effect of the present invention is believed to be due to the suppression of gene expression of IL1α and IL8, which are intracellular inflammatory mediators induced by particulate air pollutants, and further due to the suppression of gene expression of CYP1, a drug-metabolizing enzyme involved in the induction of the expression of these inflammatory mediators.

[0034] The anti-pollution agent and topical skin composition of the present invention may contain other raw materials in addition to the plant-derived polyamine-containing extract as an active ingredient, as long as the effect of the present invention is not impaired. Examples of such raw materials include water, excipients, antioxidants, preservatives, humectants, thickeners, buffers, adsorbents, solvents, emulsifiers, stabilizers, surfactants, lubricants, water-soluble polymers, sweeteners, flavorings, acidulants, and alcohols. In addition, the anti-pollution agent and topical skin composition of the present invention may contain other active ingredients in the range of the present invention that does not impair the effect of the present invention. Specific examples of active ingredients include antioxidant ingredients, anti-aging ingredients, anti-inflammatory ingredients, whitening ingredients, cell activation ingredients, vitamins, blood circulation promoting ingredients, moisturizing ingredients, ingredients that have the effect of preventing and / or repairing DNA damage, anti-glycation ingredients, peptides or derivatives thereof, amino acids or derivatives thereof, hydroquinone glycosides and esters thereof, etc.

[0035] The anti-pollution agent of the present invention can be formulated in combination with components and additives used in cosmetics, quasi-drugs, foods and beverages, pharmaceuticals and the like, in addition to the essential components of the plant-derived polyamine-containing extract, as necessary, within the scope that does not impair the effects of the present invention.

[0036] Examples of fats and oils include avocado oil, almond oil, fennel oil, perilla oil, olive oil, orange oil, orange raffaella oil, sesame oil, cacao butter, chamomile oil, carrot oil, cucumber oil, beef tallow, beef tallow fatty acids, kukui nut oil, safflower oil, soybean oil, camellia oil, corn oil, rapeseed oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, cacao butter, palm oil, palm kernel oil, Japan wax, coconut oil, beef tallow, lard, hardened oil, and hardened castor oil.

[0037] Examples of waxes include beeswax, carnauba wax, spermaceti, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax, montan wax, and shellac wax.

[0038] Examples of mineral oils include liquid paraffin, vaseline, paraffin, ozokeride, ceresin, microcrystalline wax, polyethylene powder, squalene, squalane, and pristane.

[0039] Examples of fatty acids include natural fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, and lanolin fatty acid, and synthetic fatty acids such as isononanoic acid, caproic acid, 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid, and isopentanoic acid.

[0040] Examples of alcohols include natural alcohols such as ethanol, isopropyl alcohol, lauryl alcohol, cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, and phytosterol; synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol, and 2-octyldodecanol; and polyhydric alcohols such as ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol, polypropylene glycol, 1,3-butylene glycol, glycerin, batyl alcohol, pentaerythritol, sorbitol, mannitol, glucose, and sucrose.

[0041] Examples of esters include isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyldodecyl myristate, hexyldecyl dimethyloctanoate, cetyl lactate, myristyl lactate, diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, and propylene glycol dioleate.

[0042] Examples of the metallic soap include aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, and zinc undecylenate.

[0043] Examples of gums and water-soluble polymer compounds include gum arabic, gum benzoin, gum dammar, guaiac butter, Irish moss, gum karaya, gum tragacanth, gum carob, quince seed, agar, casein, dextrin, gelatin, pectin, starch, carrageenan, carboxyalkyl chitin, chitosan, hydroxyalkyl chitin, low molecular weight chitosan, chitosan salts, sulfated chitin, phosphorylated chitin, alginic acid and its salts, hyaluronic acid and its salts, chondroitin sulfate, heparin, ethyl cellulose, methyl cellulose, carboxymethyl cellulose, carboxyethyl cellulose, sodium carboxyethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinylpyrrolidone, polyvinyl methacrylate, polyacrylates, polyalkylene oxides such as polyethylene oxide and polypropylene oxide or crosslinked polymers thereof, carboxyvinyl polymers, and polyethyleneimine.

[0044] Surfactants include anionic surfactants (carboxylates, sulfonates, sulfate salts, phosphate salts), cationic surfactants (amine salts, quaternary ammonium salts), amphoteric surfactants (carboxylate type amphoteric surfactants, sulfate ester type amphoteric surfactants, sulfonate type amphoteric surfactants, phosphate ester type amphoteric surfactants), nonionic surfactants (ether type nonionic surfactants, ether ester type nonionic surfactants, ester type nonionic surfactants, block polymer type nonionic surfactants, nitrogen-containing nonionic surfactants), and other surfactants (natural surfactants, derivatives of protein hydrolysates, polymeric surfactants, surfactants containing titanium and silicon, fluorocarbon surfactants, etc.).

[0045] Vitamins include retinol, retinal (vitamin A1), dehydroretinal (vitamin A2), carotene, and lycopene (provitamin A) in the vitamin A group; thiamine hydrochloride, thiamine sulfate (vitamin B1), riboflavin (vitamin B2), pyridoxine (vitamin B6), cyanocobalamin (vitamin B12), folic acid, nicotinic acid, pantothenic acid, biotin, choline, and inositol; ascorbic acid and its derivatives in the vitamin C group; ergocalciferol (vitamin D2), cholecalciferol (vitamin D3), and dihydrotachysterol in the vitamin D group; tocopherol and its derivatives and ubiquinones in the vitamin E group; and phytonadione (vitamin K1), menaquinone (vitamin K2), menadione (vitamin K3), and menadiol (vitamin K4) in the vitamin K group.

[0046] Examples of amino acids include valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid, hydroxylysine, arginine, ornithine, histidine, and the like, as well as amino acid derivatives thereof such as sulfates, phosphates, nitrates, citrates, or pyrrolidone carboxylic acids.

[0047] Examples of skin whitening agents include ascorbic acid or a derivative thereof, sulfur, placental hydrolysate, ellagic acid or a derivative thereof, kojic acid or a derivative thereof, glucosamine or a derivative thereof, arbutin or a derivative thereof, hydroxycinnamic acid or a derivative thereof, glutathione, arnica extract, Scutellaria root extract, Sophora japonica extract, Saiko extract, Safflower extract, Ganoderma lucidum mycelium culture or an extract thereof, Tilia extract, peach leaf extract, Angelica acutiloba extract, Sophora root extract, Jew's wort extract, persimmon leaf extract, Rhubarb extract, Peony extract, Hamamelis extract, Horse chestnut extract, Hypericum perforatum extract, and oil-soluble licorice extract.

[0048] Examples of moisturizing agents include hyaluronic acid, polyglutamic acid, serine, glycine, threonine, alanine, collagen, hydrolyzed collagen, hydronectin, fibronectin, keratin, elastin, royal jelly, chondroitin sulfate heparin, glycerophospholipid, glyceroglycolipid, sphingophospholipid, sphingoglycolipid, linoleic acid or esters thereof, eicosapentaenoic acid or esters thereof, pectin, bifidobacterium fermentation product, lactic acid fermentation product, yeast extract, ganoderma lucidum mycelium culture or extract thereof, wheat germ oil, avocado oil, rice germ oil, jojoba oil, soybean phospholipid, γ-oryzanol, bilberry extract, coix seed extract, rehmannia root extract, taiso extract, kaiso extract, aloe arborescens extract, burdock extract, rose wax extract, arnica extract, and wheat bran.

[0049] Hair growth agents include pentadecanoic acid glyceride, coleus extract, gentian extract, matsukasa extract, royal jelly extract, kumazasa extract, t-flavanone, 6-benzylaminopurine, Swertia japonica extract, carpronium chloride, minoxidil, finasteride, adenosine, nicotinamide, mulberry root extract, rehmannia extract, and 5-aminolevulinic acid.

[0050] Examples of extracts and essences from animals, plants, and herbal medicines include Acacia oleracea, Angelica gracilis, Acerola, Altea, Arnica, Avocado, Amacha, Aloe, Aloe vera, Nettle, Ginkgo biloba, Fennel, Turmeric, Asian sardine, Plum, Quercus salicina, Uva-ursi, Rosa multiflora, Astragalus chinensis, Astragalus membranaceus, Scutellaria baicalensis, Cherry bark, Phellodendron amplexicaule, Coptis japonica, Panax ginseng, Hypericum perforatum, and Origanum officinalis. Watercress, Nativity, Orange, Polygala tenuifolia, Prunella vulgaris, Houttuynia cordata, Sophora japonica, Artemisia anguicida, Zedoaria rhizome, Pueraria lobata, Valerian root, Chamomile, Melon root, Artemisia capillaris, Licorice, Coltsfoot, Rubus serrata, Kiwi fruit, Platycodon grandiflorum, Chrysanthemum flower, Catalpa fruit, Citrus fruit, Tachibana peel, Cucumber, Aralia udo or Angelica aralia, Apricot kernel, Kudzu, Goji bark, Goji berries, Goji leaves, Sophora flavonoides, Camphor tree, Kumazasa, Grapefruit fruit, Cinnamon bark, Cinnamon bark, Cassia jasmine, Round morning glory or Japanese morning glory, Safflower, Comfrey, Copaiba, Gardenia, Gentian, Magnolia obovata, Achyranthes jasmine, Euonymus chinensis, Burdock, Schisandra chinensis, Rice, Rice bran, Wheat, Bupleurum fructus, Saffron, Soapwort, Crataegus crenata, Japanese pepper Pepper), Salvia, Sanshichi ginseng, Shiitake mushroom, Rehmannia rhizome, Shikunshi, Purple root, Perilla frutescens, Chinese peony, Plantain, Ginger, Calamus, Ligusticum japonica, Spiraea gracilis, Japanese white birch, Honeysuckle, Ivy, Yarrow, Elderberry, Adzuki bean, Elderberry, Mallow, Cnidium officinalis, Swertia japonica, Mulberry bark,Mulberry leaves), jujube, soybean, elm, bamboo joint ginseng, anemone, elm, houttuynia cordata, cordyceps sinensis, chili pepper, ground cherries, thyme, green tea, black tea, cloves, unshu mandarin oranges, camellia, centella, chili pepper, angelica, calendula , bitter orange (orange skin), serroko (elm), corn (southern elm), eucommia ulmoides (Eucommia ulmoides, leaves), tomato, nandina (nanten fruit), garlic, barley (malt), hakusan (white moss skin), ophiopogon (Ophiopogon), parsley, batata (batata), mentha (peppermint), hamamelis, rose, loquat leaf, matsuhodo (Japanese holly), grape or its leaves, loofah, linden tree, peony (peony skin), hops, maikai (Magnolia), pine needles, horse chestnut, rosemary, soapberry, melissa, melilot, Japanese quince, bean sprouts, peach kernel, peach leaf, bean sprout, areca palm, areca nut, amplexicaule, centaurea, saxifrage, bayberry, alder bark, coix seed, mugwort, Japanese mugwort, lavender, apple fruit, Ganoderma lucidum, lemon fruit These include forsythia fruit, astragalus, geranium, rhizome, rooster comb, bovine and human placenta extracts, porcine and bovine stomach, duodenum, or intestine extracts or hydrolyzates thereof, water-soluble collagen, water-soluble collagen derivatives, collagen hydrolyzates, elastin, elastin hydrolyzates, water-soluble elastin derivatives, silk protein, silk protein hydrolyzates, and bovine blood cell protein hydrolyzates.

[0051] Examples of microbial culture metabolites include yeast extract, zinc-containing yeast extract, germanium-containing yeast extract, selenium-containing yeast extract, magnesium-containing yeast extract, rice fermentation extract, Euglena extract, and lactic acid fermentation products of skim milk powder.

[0052] Alpha-hydroxy acids include glycolic acid, citric acid, malic acid, tartaric acid, and lactic acid.

[0053] Examples of inorganic pigments include silicic anhydride, magnesium silicate, talc, kaolin, bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, red iron oxide, black iron oxide, ultramarine, chromium oxide, chromium hydroxide, carbon black, and calamine.

[0054] Examples of ultraviolet absorbers include p-aminobenzoic acid derivatives, salicylic acid derivatives, anthranilic acid derivatives, coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives, nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives, vitamin B6 derivatives, oxybenzone, benzophenone, guaiazulene, shikonin, baicalin, baicalein, and berberine.

[0055] Astringents include lactic acid, tartaric acid, succinic acid, citric acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, potassium aluminum sulfate, resorcin, ferric chloride, and tannic acid.

[0056] Antioxidants include ascorbic acid and its salts, stearic acid esters, tocopherol and its ester derivatives, nordihydroguaiaretic acid, butylhydroxytoluene (BHT), butylhydroxyanisole (BHA), parahydroxyanisole, propyl gallate, sesamol, sesamolin, gossypol, and the like.

[0057] Anti-inflammatory agents include ichthammol, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d- or dl-camphor, hydrocortisone, guaiazulene, chamazulene, chlorpheniramine maleate, glycyrrhizic acid and its salts, glycyrrhetinic acid and its salts, and the like.

[0058] Bactericides and disinfectants include acrinol, sulfur, benzalkonium chloride, benzethonium chloride, methylrosaniline chloride, cresol, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or its hydrolysates.

[0059] Hair care products include selenium disulfide, alkylisoquinolinium bromide solution, zinc pyrithione, biphenamine, thianthol, castor tincture, ginger tincture, capsicum tincture, quinine hydrochloride, strong aqueous ammonia, potassium bromate, sodium bromate, and thioglycolic acid.

[0060] Fragrances include natural animal fragrances such as musk, civet, castoreum, and ambergris, anise essential oil, angelica essential oil, ylang ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, guaiac wood essential oil, cumin essential oil, black letter essential oil, cassia bark essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriander essential oil, shiso essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, ginger grass essential oil, cedar essential oil, spearmint essential oil, and Western Examples of plant-based fragrances include peppermint essential oil, aniseed essential oil, tuberose essential oil, clove essential oil, orange flower essential oil, wintergreen essential oil, tolu balsam essential oil, butterbur essential oil, rose essential oil, palmarosa essential oil, cypress essential oil, Japanese cypress essential oil, sandalwood essential oil, petitgrain essential oil, bay essential oil, vetiver essential oil, bergamot essential oil, Peru balsam essential oil, bois de rose essential oil, camphor essential oil, mandarin essential oil, eucalyptus essential oil, lime essential oil, lavender essential oil, linaloe essential oil, lemongrass essential oil, lemon essential oil, rosemary essential oil, and Japanese peppermint essential oil, as well as other synthetic fragrances.

[0061] Examples of pigments and coloring agents include red cabbage pigment, red rice pigment, madder pigment, annatto pigment, squid ink pigment, turmeric pigment, sophora japonica pigment, krill pigment, persimmon pigment, caramel, gold, silver, gardenia pigment, corn pigment, onion pigment, tamarind pigment, spirulina pigment, whole buckwheat pigment, cherry pigment, seaweed pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple sweet potato pigment, purple yam pigment, lac pigment, and rutin.

[0062] Sweeteners include sugar, sweet tea, fructose, arabinose, galactose, xylose, mannose, maltose, honey, glucose, miraculin, monellin, and the like.

[0063] Nutritional fortifiers include calcined shell calcium, cyanocolabamin, yeast, wheat germ, soybean germ, egg yolk powder, hemicellulose, and heme iron.

[0064] Other examples include hormones, sequestering agents, pH adjusters, chelating agents, preservatives and antimicrobial agents, cooling agents, stabilizers, emulsifiers, animal and plant proteins and their decomposition products, animal and plant polysaccharides and their decomposition products, animal and plant glycoproteins and their decomposition products, blood flow promoters, anti-inflammatory and anti-allergic agents, cell activators, keratolytic agents, wound healing agents, foaming agents, thickeners, oral agents, deodorizing agents, bittering agents, seasonings, enzymes, etc.

[0065] The anti-pollution agent of the present invention may be in any dosage form, and may be used by blending it with quasi-drugs such as ampoules, capsules, powders, granules, pills, tablets, solids, liquids, gels, foams, emulsions, creams, ointments, sheets, and mousses, cosmetics for the skin and hair, bath preparations, foods, beverages, and medicines.

[0066] Specific examples of cosmetics and quasi-drugs include basic cosmetics such as internal and external medicinal preparations, lotions, milky lotions, creams, ointments, lotions, oils and packs, facial cleansers and skin cleansers, hair cosmetics such as shampoos, rinses, hair treatments, hair creams, pomades, hair sprays, hair styling products, perm products, hair tonics, hair dyes, hair growth and care products, make-up cosmetics such as foundations, face powders, powders, lipsticks, blushers, eye shadows, eyeliners, mascara, eyebrow inks and eyelashes, finishing cosmetics such as nail beautifiers, perfumes, bath products, and more.

[0067] Examples of food and beverages include beverages such as soft drinks, carbonated drinks, nutritional drinks, fruit drinks, and lactic acid drinks; cold desserts such as ice cream, ice sherbet, and shaved ice; noodles such as soba, udon, harusame, gyoza wrappers, shumai wrappers, Chinese noodles, and instant noodles; sweets such as candy, candy, gum, chocolate, tablet candy, snacks, biscuits, jelly, jam, cream, baked goods, and bread; seafood such as crab, salmon, clams, tuna, sardines, shrimp, bonito, mackerel, whale, oysters, pacific saury, squid, ark shells, scallops, abalone, sea urchin, salmon roe, and tokobushi; kamaboko, ham, sausages, etc. These include processed seafood and livestock foods, dairy products such as milk powder, processed milk, fermented milk, salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, dressings and other oils and fats and processed oil foods, seasonings such as sauces and dressings, retort pouch foods such as curry, stew, oyakodon, porridge, zosui, Chinese rice bowls, katsudon, tendon, unadon, hayashi rice, oden, mabo dolph, beef bowls, meat sauce, egg soup, omelet rice, gyoza, shumai, hamburger steaks, meatballs, various forms of health and nutritional supplements, health functional foods, tablets, capsules, drinks, troches, etc.

[0068] The antipollution agent of the present invention and the external skin composition containing said antipollution agent are preferably applied to humans, but can also be applied to animals other than humans as long as the respective functional effects can be expected.

[0069] The embodiment of the skin external composition of the present invention is not particularly limited, and examples thereof include face wash, cleanser, lotion (e.g., whitening lotion), cream (e.g., vanishing cream, cold cream), milky lotion, gel, beauty essence, pack (e.g., jelly-like peel-off type, paste-like wipe-off type, powder-like wash-off type), face mask, cleansing, foundation, lipstick, lip balm, lip gloss, lip liner, blusher, makeup base, shaving lotion, sunscreen, after-sun lotion, deodorant lotion, body lotion (including hand care lotion and foot care lotion), body oil, etc. In particular, from the viewpoint of anti-pollution, a skin external composition used as a leave-on type during the day is preferred, and examples thereof include lotion, cream, milky lotion, gel, beauty essence, foundation, makeup base, sunscreen, body lotion, body oil, etc.

[0070] The present invention will be described in more detail below with reference to examples, although the present invention is not limited to these examples. EXAMPLES

[0071] (Example 1) Preparation of plant extract containing polyamine from soybean germ and / or plant extract containing polyamine as an active ingredient A diluted internal standard solution (1,7-diaminoheptane, internal standard amount = 10 μmol) and 5 L of 1N hydrochloric acid solution were added to 1 kg of soybean germ (Four You), and the mixture was left at room temperature for 1 hour. Then, 80 g of polyphenol adsorbent Polyclar VT (ISP) was added, and the soybean germ was thoroughly crushed with a homogenizer, and the mixture was left at room temperature for 1 hour to extract under acidic conditions. The crushed material was centrifuged at 2°C and 22,000 x g for 30 minutes to collect the liquid fraction, which was neutralized with 30% sodium hydroxide solution to obtain the plant extract (soybean germ extract). The plant extract (soybean germ extract) was desalted using an electrodialysis device (Acilyser, Astom), and concentrated by freeze-drying. This powder was dissolved in a 30% butylene glycol solution to a concentration of 5.0%, and used as a soybean extract for various evaluations.

[0072] (Example 2) Evaluation of the effect of suppressing expression of a target gene The soybean extract was used as a test sample to evaluate the expression-inhibiting effect on target genes by the following test method. In this example, the target genes refer to the IL1α gene, the IL8 gene, and the CYP1 gene.

[0073] Human normal neonatal epidermal keratinocytes (NHEK) were seeded on a collagen-coated T75 flask using Collagen Coating Solution (manufactured by Toyobo Co., Ltd.), and cultured at 37°C and 10% CO2 after adding normal human epidermal keratinocyte growth medium (HuMedia-KG2). Culture was continued for 8 days with appropriate medium changes until the cells were 80% or more confluent. After removing the medium and rinsing with PBS, the cells were harvested by trypsinization. 1 × 10 4 The cells were seeded at 10 cells / well and cultured at 37°C, 10% CO2 for one day.

[0074] The medium was removed, replaced with a medium containing the test sample at a predetermined concentration, and incubated at 37°C, 10% CO2 for 5 hours. In addition to the test sample, dexamethasone, known as an anti-inflammatory agent, was dissolved in DMSO and used as a positive control (final concentration 1 μM). PM2.5 extract (200 mg / ml) was added to a final concentration of 0.2 mg / ml and incubated for another 2 hours. PM2.5 extract was prepared by adding 1 mL of DMSO to 200 mg of urban air dust (NIES CRM No. 28), stirring and centrifugation, and collecting the supernatant. After culturing, the cells were collected, and cDNA was prepared from the cells using SuperPrep (registered trademark) Cell Lysis & RT Kit for qPCR (manufactured by Toyobo Co., Ltd.).

[0075] Based on the obtained cDNA, THUNDERBIRD SYBR (registered trademark) qPCR Mix (manufactured by Toyobo Co., Ltd.) was used to amplify the mRNA gene by qRT-PCR. The reaction solution composition was in accordance with the attached document. However, the reaction solution volume was 20 μl / well, and the cDNA volume was 3 μl. The reaction cycle conditions were 95°C, 1 minute → (95°C, 15 seconds → 60°C, 45 seconds) × 40 → 95°C, 15 seconds → 60°C, 1 minute → 95°C, 15 seconds. The primers used were oligonucleotides consisting of the base sequences shown in SEQ ID NOs: 1 to 8. The 7500 Fast Real-Time PCR System (Applied Biosystems) was used for gene amplification. The glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene was used as an internal standard to standardize the expression level of the target gene. The standardized expression level of each target gene was divided by the expression level of the gene (Untreated control) confirmed in the same manner as above, except that PM2.5 was not added, to calculate the relative value.

[0076] The results are shown in Figures 1 to 3. As can be seen from Figures 1 to 3, in NHEK, in the negative control to which no soybean extract was added, the expression levels of the IL1α gene, IL8 gene, and CYP1 gene were enhanced by particulate air pollutants compared to the untreated control, whereas the expression levels of the IL1α gene, IL8 gene, and CYP1 gene were reduced by the addition of the plant-derived polyamine-containing extract. These results demonstrate that the plant-derived polyamine-containing extract of the present invention suppresses the enhanced expression of the IL1α gene, IL8 gene, and CYP1 gene induced by PM2.5 in epidermal cells, and suppresses inflammatory responses.

[0077] Example 3: Preparation of a skin topical composition Examples of formulations for external skin preparations of the present invention are given below.

[0078] (1) Lotion A lotion was prepared in a conventional manner according to the following composition. ·Purified water ···89.80g Glycerin: 3.00 g Phenoxyethanol: 0.20 g Butylene glycol 5.00 g Pentylene glycol 1.00 g Soybean extract: 1.00g

[0079] (2) Gel A gel was prepared by conventional methods according to the following composition: Purified water: 88.50g Carbomer 0.30 g Xanthan gum 0.10g Arginine: 0.40 g Glycerin: 5.00 g Phenoxyethanol: 0.20 g Butylene glycol 5.00 g Soybean extract: 0.50g

[0080] (3) Cream A cream was prepared in a conventional manner according to the following composition. Purified water: 58.50g Butylene glycol 10.00 g Glycerin: 5.00 g Phenoxyethanol: 0.20 g Ethylhexylglycerin: 0.20g Squalane: 10.00g Olive oil: 10.00g Behenyl alcohol 2.50g Polyglyceryl-10 pentastearate 1.90g Sodium stearoyl lactate 0.60g Cetyl palmitate 1.00g Soybean extract: 0.10g [Industrial Applicability]

[0081] These preparations are expected to suppress the expression of IL1α gene, IL8 gene, and CYP1 gene, which are upregulated by PM2.5 in epidermal cells, and are effective as anti-pollution agents, particularly as ingredients of topical skin preparations.

Claims

1. A method for using a plant-derived polyamine-containing extract, which is an extract derived from at least one material selected from the group consisting of soybean seeds, soybean germs, soybean embryos, soybean sprouts, wheat seeds, wheat germs, wheat embryos, wheat sprouts, soy milk, and okara, in the manufacture of an anti-pollution agent for preventing or suppressing skin inflammation by suppressing the expression of interleukin-1α (IL1α), interleukin-8 (IL8) or cytochrome P450 (CYP1) in epidermal cells caused by particulate air pollutants.

2. 2. The method for producing an anti-pollution agent according to claim 1, wherein the plant-derived polyamine-containing extract is an extract derived from soybean germ.

3. 3. A method for using a plant-derived polyamine-containing extract containing at least one compound selected from the group consisting of putrescine, spermidine and spermine as a polyamine in the production of an antipollution agent according to claim 1 or 2.

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