Treatment of immune disorders

Abstract

To increase production of anti-inflammatory cells in human subjects using genetically unmodified stem cells that express Ang1 at high levels without the need for transfection of the cells with a nucleic acid expressing Ang1.SOLUTION: The present disclosure relates to stem cells which express high levels of Angiopoietin-1 (Ang1), and uses thereof in inhibiting M1-type macrophage production and treating inflammatory disease such as diabetes.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present disclosure relates to stem cells expressing high levels of angiopoietin-1 (Ang1) and their use in inhibiting the release of TNF-α and / or IL-6 and treating inflammatory diseases such as diabetes. [Background technology]

[0002] Angiopoietins are part of a family of vascular growth factors involved in early and postnatal angiogenesis. Ang1 promotes migration of endothelial cells and some non-endothelial cells, such as smooth muscle cells. Ang1 also induces endothelial cell sprouting and remodeling into renal tubules. Ang1 exerts a potent anti-inflammatory effect on endothelial cells, suppressing vascular endothelial growth factor (VEGF) inducing upregulation of E-selectin, ICAM-1 and VCAM-1, and inhibiting leukocyte adhesion and transmigration in response to VEGF and TNF-α (Kim et al., Circ Res., 89(6), 477-479, 2001).

[0003] Current treatment for the majority of type I diabetes patients is based on regular subcutaneous injections of a mixture of fast- and long-acting insulin preparations to achieve a constant basal level of the hormone by administering a suspension of soluble insulin particles of different sizes, providing intermediate- and long-acting components with a more sustained profile of action (Heine et al., Br Med J (Clin Res Ed) 290:204-205, 1985).

[0004] The drawback of this current therapy is that sustained release formulations generally do not produce a smooth background level of insulin, resulting in either hyperglycemia or hypoglycemia. Hyperglycemia is problematic in diabetic patients because it can lead to further complications. For example, chronic hyperglycemia leads to severe microvascular (retinopathy and nephropathy), macrovascular (stroke, myocardial infarction) and neurological complications. These serious complications can be prevented by normalizing blood glucose levels.

[0005] In recent years, stem cell-based technologies have emerged as a potential approach to treat diabetes, however, in addition to issues related to the underlying autoimmune disease that may require lifelong immune control, these technologies have yet to emerge as viable therapeutic options.

[0006] Thus, there remains an unmet therapeutic need among patients suffering from diabetes and / or its related conditions or symptoms that require new treatment options. Citation or identification of any document in this application is not an admission that such document is available as prior art to the present disclosure. [Prior art documents] [Non-patent literature]

[0007] [Non-Patent Document 1] Kim et al., Circ Res., 89(6), 477-479, 2001 [Non-Patent Document 2] Heine et al. Br Med J (Clin Res Ed) 290:204-205, 1985 Summary of the Invention

[0008] The inventors have found that using genetically unmodified stem cells that express high levels of Ang1, it is possible to increase the production of anti-inflammatory cells in human subjects without the need to transfect the cells with nucleic acids that express Ang1.

[0009] The inventors also found that it was possible to decrease TNF-alpha levels, decrease IL-6 levels, and / or increase IL-10 levels in human subjects. These findings suggest that unaltered stem cells expressing such high levels of Ang1 may be suitable for treating inflammatory diseases such as diabetes and its related conditions and symptoms.

[0010] Indeed, the inventors have found that using genetically unmodified stem cells that express high levels of Ang1, it is possible to reduce HbA1c, reduce fasting insulin levels and / or increase adiponectin levels in human subjects suffering from diabetes, without the need to transfect the cells with a nucleic acid expressing Ang1.

[0011] In other examples, the inventors have also shown that unmodified stem cells expressing high levels of Ang1 can be used to ameliorate symptoms of rheumatoid arthritis and diabetic nephropathy in human subjects without the need to transfect the cells with nucleic acid expressing Ang1.

[0012] Thus, in one embodiment, the disclosure provides a method of increasing the production and / or function of anti-inflammatory cells in a subject in need thereof, the method comprising administering to the subject a composition comprising ungenetically modified stem cells, the ungenetically modified stem cells being at least 0.1 μg / 10 6 A large amount of cells express angiopoietin-1 (Ang1).

[0013] In one embodiment, the anti-inflammatory cell is a Th2 cell, a TReg cell, or an M2-type macrophage.

[0014] In another embodiment, the method increases the number of M2-type macrophages in the subject.

[0015] In another embodiment, the method increases the number of M2-type macrophages in the subject to at least 10% of the total mononuclear cell population.

[0016] In another embodiment, the method increases the number of M2-type macrophages in the subject to at least 20% of the total mononuclear cell population.

[0017] In another embodiment, the method increases the number of M2-type macrophages to at least 40% of the total mononuclear cell population in the subject.

[0018] In another embodiment, the method increases the number of M2-type macrophages to at least 80% of the total mononuclear cell population in the subject.

[0019] In another embodiment, the method increases the number of M2-type macrophages to at least 90% of the total mononuclear cell population in the subject.

[0020] In another embodiment, M2 type macrophages are CD14+CD16+.

[0021] In another embodiment, M2 type macrophages are CD14+CD16+CD163+.

[0022] In another embodiment, M2 type macrophages are CD14+CD16+CD206+.

[0023] In another embodiment, M2 type macrophages are CD14+CD16+CD163+CD206+.

[0024] In another embodiment, M2 type macrophages are CD14++CD16+.

[0025] In another embodiment, M2 type macrophages are CD14++CD16+CD163+.

[0026] In another embodiment, M2 type macrophages are CD14++CD16+CD206+.

[0027] In another embodiment, M2 type macrophages are CD14++CD16+CD163+CD206+.

[0028] In another embodiment, the method promotes polarization of macrophages from an M1 to an M2 phenotype.

[0029] In another embodiment, the method promotes differentiation of proinflammatory helper T cells into Th2 cells or TReg cells.

[0030] In one embodiment, the proinflammatory helper T cells are Th17 cells.

[0031] In one embodiment, increasing the production and / or function of anti-inflammatory cells in a subject is A reduction in IL-6 levels in a subject; A reduction in TNF-alpha levels in a subject; and / or · Causing an increase in IL-10 levels in a subject.

[0032] In one example, the method increases the level of an anti-inflammatory cytokine in the subject.

[0033] In one example, the method increases the level of IL-10 in the subject.

[0034] In one example, the method reduces the levels of pro-inflammatory cytokines in the subject.

[0035] In one example, the method reduces the level of any one of IL-6, TNF-alpha and / or IL-17 in a subject.

[0036] In one embodiment, the method also inhibits the production and / or function of pro-inflammatory cells.

[0037] In one embodiment, the proinflammatory cells are Th17 cells or M1 type macrophages.

[0038] In another embodiment, the disclosure provides a method of inhibiting M1 macrophage polarization.

[0039] In another embodiment, the present disclosure provides a method for promoting polarization of macrophages from an M1 to an M2 phenotype.

[0040] In another embodiment, the present disclosure provides a method of inhibiting cytokine release from M1 type macrophages.

[0041] In another embodiment, the disclosure provides a method, wherein the M1-type macrophage-derived cytokine that is inhibited is TNF-alpha and / or IL-6.

[0042] In another embodiment, the disclosure provides a method of treating an inflammatory disease, the method comprising administering to a subject a composition comprising unaltered stem cells, the unaltered stem cells being at least 0.1 μg / 10 6 A large amount of cells express angiopoietin-1 (Ang1).

[0043] In another embodiment, the inflammatory disease is diabetes or a diabetes-related condition or symptom selected from the group consisting of abnormal wound healing, heart attack symptoms, stroke symptoms, peripheral vascular disease symptoms, amputation, kidney disease symptoms, kidney failure, blindness, neuropathy, nephropathy, retinopathy, inflammation, impotence, or non-alcoholic steatohepatitis (NASH).

[0044] For example, the disclosure provides a method of treating rheumatoid arthritis.

[0045] For example, the present disclosure provides a method of treating diabetic retinopathy.

[0046] In another embodiment, the method of the present disclosure comprises administering to a subject a composition comprising ungenetically modified stem cells, the ungenetically modified stem cells being at least 0.1 μg / 10 6 In another embodiment, the stem cells express angiopoietin-1 (Ang1) in an amount of at least 0.5 μg / 10 6 In another embodiment, the stem cells express Ang1 in an amount of at least 0.7 μg / 10 6 In another embodiment, the stem cells express Ang1 in an amount of at least 1 μg / 10 6 The amount of cells expressing Ang1.

[0047] In another embodiment, the stem cells are at about 0.1 μg / 10 6In another embodiment, the stem cells express VEGF at levels less than about 0.05 μg / 10 6 In another embodiment, the stem cells express VEGF at levels less than about 0.04 μg / 10 6 In another embodiment, the stem cells express VEGF at levels less than about 0.03 μg / 10 6 In another embodiment, the stem cells express VEGF at levels less than about 0.02 μg / 10 6 In another embodiment, the stem cells express VEGF in amounts less than about 0.01 μg / 10 6 They express VEGF in subcellular amounts.

[0048] In another embodiment, the stem cells express Ang1:VEGF in a ratio of at least about 2:1. In another embodiment, the stem cells express Ang1:VEGF in a ratio of at least about 10:1. In another embodiment, the stem cells express Ang1:VEGF in a ratio of at least about 20:1. In another embodiment, the stem cells express Ang1:VEGF in a ratio of at least about 30:1. In another embodiment, the stem cells express Ang1:VEGF in a ratio of at least about 50:1.

[0049] In another embodiment, the stem cells are mesenchymal stem cells. In another embodiment, the stem cells are mesenchymal progenitor cells. In another embodiment, the stem cells are induced pluripotent stem cells (iPS cells).

[0050] In another embodiment, the composition further comprises an acceptable pharmaceutical carrier.

[0051] In another embodiment, the composition is produced by culturing non-genetically modified stem cells according to the in vitro methods described below.

[0052] In one embodiment, the stem cells can be obtained from any mammal, for example, the stem cells may be from a primate, cow, sheep, horse, dog, cat, or goat, hi another embodiment, the stem cells are human stem cells.

[0053] In another embodiment, the inflammatory disease is type II diabetes.

[0054] In one embodiment, the method for treating type II diabetes includes administering about 0.1×10 6 pieces~approx. 3×10 6 This may include administering stem cells to the subject.

[0055] In one embodiment, the method for treating type II diabetes includes administering about 0.3×10 6 pieces ~ approx. 2×10 6 This may include administering stem cells to the subject.

[0056] In one embodiment, the method for treating type II diabetes includes administering about 1×10 6 pieces ~ approx. 2×10 6 This may include administering stem cells to the subject.

[0057] In one embodiment, the method for treating type II diabetes includes administering about 2×10 6 This may include administering stem cells to the subject.

[0058] In one embodiment, diabetes or a diabetes related condition or symptom is treated in a subject by any one of the following: · Reduction in HbA1c levels (percentage of total hemoglobin); · Reduction in fasting insulin levels; Decreased IL-6 levels; A reduction in TNF-α levels; and / or Increased adiponectin levels

[0059] In one embodiment, the subject has type II diabetes and the subject's glucose levels are poorly controlled.

[0060] In one embodiment, the subject's glucose levels are not adequately controlled by metformin.

[0061] In one example, the subject has a baseline HbA1c value of greater than 7.5%.

[0062] In one embodiment, the subject has a baseline HbA1c value of 8% or greater.

[0063] In one embodiment, the inflammatory disease is rheumatoid arthritis.

[0064] In one embodiment, the method for treating rheumatoid arthritis comprises administering about 0.5×10 per kg of 6 pieces ~ approx. 3.0×10 6 This may include administering stem cells to the subject.

[0065] In one embodiment, the method for treating rheumatoid arthritis comprises administering about 1.0×10 6 pieces ~ approx. 2.0×10 6 This may include administering stem cells to the subject.

[0066] In one embodiment, rheumatoid arthritis is treated as indicated by any one of the following: ·ACR20; ·ACR50 ACR70 Decreased levels of IL-6; and / or -Reduction in Disease Activity Score

[0067] In another embodiment, the inflammatory disease is diabetic neuropathy.

[0068] In one embodiment, the method of treating rheumatoid arthritis comprises administering about 1.0×10 8 pieces ~ approx. 4.0×10 8 This may include administering stem cells to the subject.

[0069] In one embodiment, the method of treating rheumatoid arthritis comprises administering to a subject a dose of about 1.5×10 8 pieces ~ approx. 3.0×10 8 This may include administering stem cells to the subject.

[0070] In one embodiment, diabetic nephropathy is treated as indicated by any one of the following: Inhibition of decline in eGFR and / or mGFR; improvement in eGFR and / or mGFR; and / or Reduced IL-6 levels

[0071] In one embodiment, the subject's baseline eGFR is about 35 ml / min / 1.73 m 2 Greater than.

[0072] In one embodiment, the subject's baseline eGFR is 30 ml / min / 1.73 m 2 Greater than.

[0073] In one embodiment, the subject's baseline eGFR is 28 ml / min / 1.73 m 2 Greater than.

[0074] In one embodiment, the subject's baseline eGFR is 25 ml / min / 1.73 m 2 Greater than.

[0075] In one embodiment, the stem cells are administered systemically.

[0076] In one embodiment, the stem cells are administered intravenously.

[0077] In another embodiment, the disclosure relates to the use of a composition comprising ungenetically modified stem cells, wherein the ungenetically modified stem cells express high levels of angiopoietin-1 (Ang1), in the manufacture of a medicament for treating an inflammatory disease.

[0078] In another embodiment, the present disclosure relates to a composition comprising genetically unmodified stem cells for use in treating an inflammatory disease, wherein the genetically unmodified stem cells express high levels of angiopoietin-1 (Ang1).

[0079] In another embodiment, the present disclosure relates to a composition comprising genetically unmodified stem cells, which express high levels of angiopoietin-1 (Ang1) when used to treat an inflammatory disease. [Brief description of the drawings]

[0080] [Figure 1] MPC proliferation in current culture medium (Process A) and reformulated culture medium (Process B). The Y-axis shows cell number and the X-axis is time in days. Control medium is the culture medium used in Process A and reformulated medium is the culture medium used in Process B. [Diagram 2] MPC doubling time in current medium (Process A) and reformulated medium (Process B). MPCs were expanded in current alpha MEM culture (Process A) or reformulated culture of modified alpha MEM (Process B). Cells were passaged from P3 to P5. [Diagram 3] Population doubling time (PDL) of MPCs. MPCs were expanded from P3 to P5 in current alpha-MEM culture medium (Process A) and reformulated alpha-MEM (Process B). MPCs expanded in current medium (Process A) reached 8 PDL and MPCs expanded in reformulated culture medium (Process B) reached 7.33 PDL. [Figure 4] Co-culture of immunoselected CD14+ monocytes with MPCs gives rise to CD14+16+ macrophages that display phenotypic characteristics of M2 macrophages. [Diagram 5] Co-culture with MPC blocks pro-inflammatory (LPS) secretion of TNFα by macrophages. [Figure 6] Co-culture with MPC in the presence of LPS enhanced the production of the anti-inflammatory cytokine IL-10 by macrophages. [Figure 7] MPC plays a role in regulating macrophage polarization. [Figure 8] PGE2 secretion: additive effect of IL-1β and TNFα. [Figure 9] Changes in the levels of pro- and anti-inflammatory cytokines following MPC administration. [Figure 10-1] Administration of MPC at the time of establishment of late stage disease (42 days) results in reduced levels of inflammatory cytokines in the joints. [Figure 10-2] Administration of MPC at the time of establishment of late stage disease (42 days) results in reduced levels of inflammatory cytokines in the joints. [Figure 10-3] Administration of MPC at the time of establishment of late stage disease (42 days) results in reduced levels of inflammatory cytokines in the joints. [Figure 11] MPC dose for cohorts 1–3. [Figure 12] Adiponectin levels at 12 weeks (change from baseline). [Figure 13] TNF-alpha levels at 12 weeks (change from baseline). [Figure 14] IL-6 levels (change from baseline) at week 12. [Figure 15-1] HbA1c change by baseline HbA1c subgroups <8% or ≥8% [Figure 15-2] HbA1c change by baseline HbA1c subgroups <8% or ≥8% [Figure 16] Subjects who achieved the target HbA1c (<7.0%) at week 12 [Figure 17-1] Median IL-6 levels over time [Figure 17-2] Median IL-6 levels over time [Figure 18] ACR20 response in biologics-refractory RA: 1MMPC / kg [Figure 19] ACR50 response in biologics-refractory RA: 1MMPC / kg [Figure 20] ACR70 response in biologics-refractory RA: 1MMPC / kg [Figure 21] Response over time of ACR by component; number of tender joints (TJC; left) and swollen joints (SJC; right) per week [Figure 22]Response over time of ACR by component; weekly Subjective Global Assessment of Disease Activity (SGADA) (left); weekly Physician Global Assessment of Disease Activity (IGADA) (right) [Figure 23] DASCRP Responder Analysis at Week 12 [Figure 24] Health Assessment Questionnaire Disability Index (HAQ-DI): MCID achievement rate (-0.22) [Diagram 25] Changes from baseline in mGFR [99Tc DTPA] and eGFR [MDRD at 12 weeks (ml / min / 1.73m2) [Figure 26] Median change from baseline in IL-6 at week 12 [Figure 27] Baseline IL-6 correlated with serum creatinine improvement at week 12 in MPC-treated patients [Figure 28] Change from baseline in mGFR and eGFR at Week 12 (subjects with baseline eGFR > 30ml / min / 1.73m2) DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0081] General Techniques and Definitions Throughout this specification, unless otherwise indicated or required by context, a reference to a single step, composition of matter, group of steps or group of compositions of matter should be interpreted as encompassing a plurality (i.e. one or more) of that step, composition of matter, group of steps or group of compositions of matter.

[0082] Those skilled in the art will appreciate that the disclosure described herein is susceptible to variations and modifications other than those specifically described. It is to be understood that the present disclosure includes all such variations and modifications. The present disclosure also includes all of the steps, features, compositions and compounds referred to or shown herein, individually or collectively, and any and all combinations or any two or more of said steps or features.

[0083] The present disclosure is not to be limited in scope by the specific embodiments described herein, which are intended for purposes of illustration only. Functionally equivalent products, compositions and methods are clearly within the scope of the present disclosure as described herein.

[0084] Unless specifically stated otherwise, any embodiment disclosed herein should be construed as applying mutatis mutandis to any other embodiment.

[0085] Unless otherwise defined, all technical and scientific terms used herein should be understood to have the same meaning as commonly understood by one of ordinary skill in the art (e.g., of cell culture, molecular genetics, stem cell differentiation, immunology, immunohistochemistry, protein chemistry, and biochemistry).

[0086] Unless otherwise indicated, the stem cell, cell culture, and immunological techniques utilized in this disclosure are standard procedures, well known to those skilled in the art. Such techniques are described and explained throughout the literature in such sources as J. Perbal, A Practical Guide to Molecular Cloning, John Wiley & Sons (1984), J. Sambrook et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbour Laboratory Press (1989), TA Brown (ed.), Essential Molecular Biology: A Practical Approach, volumes 1 and 2, IRL Press (1991), DM Glover and BD Hames (eds.), and FM Ausubel et al. (eds.), Current Protocols in Molecular Biology, Greene Pub. Associates and Wiley-Interscience (1988, including all updates until present), Ed Harlow and David Lane (eds.), Antibodies: A Laboratory Manual, Cold Spring Harbour Laboratory, (1988), and JE Coligan et al. (eds.), Current Protocols in Immunology, John Wiley & Sons (including all updates until present).

[0087] The term "and / or" should be interpreted as explicitly supporting both meanings or either meaning, for example, "X and / or Y" should be understood to mean either "X and Y" or "X or Y."

[0088] As used herein, unless stated to the contrary, the term "about" means + / - 10%, more preferably + / - 5% of the specified value.

[0089] The definition of volume percent (v / v%) is [(volume of solute) / (volume of solution)] × 100%. Volume percent is proportional to the volume of the solution. For example, supplementing a cell culture medium with 5% (v / v) FCS means that there is approximately 5ml FCS for every 100ml of cell culture medium.

[0090] Throughout this specification the word "comprise", or variations such as "comprises" or "comprising", will be understood to be meant to include a stated element, integer or step, or group of elements, integers or steps, but not to exclude any other element, integer or step, or group of elements, integers or steps.

[0091] stem cells As used herein, the term "stem cell" refers to a self-renewing cell that can give rise to phenotypically and genotypically identical daughters and at least one other ultimate cell type (e.g., a terminally differentiated cell). The term "stem cell" includes totipotent, pluripotent and multipotent cells as well as progenitor and / or precursor cells derived from their differentiation. Stem cells may be adult or embryonic stem cells.

[0092] As used herein, the term "totipotent cell" or "totipotential cell" refers to a cell that is capable of forming a complete embryo (eg, a blastula).

[0093] As used herein, the term "pluripotent cell" or "pluripotential cell" refers to a cell that has the full differentiation potential, i.e., the ability to develop into any of the approximately 260 cell types in the mammalian body. Pluripotent cells can self-renew and remain dormant or quiescent within a tissue.

[0094] "Multipotential cell" or "multipotent cell" refers to a cell that can give rise to multiple mature cell types. As used herein, the term encompasses adult progenitor cells and the multipotent progeny of these cells. Unlike pluripotent cells, multipotent cells do not have the ability to form all of the cell types.

[0095] As used herein, the term "mesenchymal progenitor or stem cell" refers to a cell that can differentiate into a mesenchymal cell type. For example, mesenchymal progenitor and precursor cells can differentiate into bone tissue, cartilage tissue, muscle tissue, and fat cells, and fibrous connective tissue.

[0096] In one embodiment, the stem cells expressing high levels of Ang1 are STRO-1+ mesenchymal progenitor cells.

[0097] STRO-1+ multipotent cells are cells found in bone marrow, blood, dental pulp, adipose tissue, skin, spleen, pancreas, brain, kidney, liver, heart, retina, brain, hair follicle, intestine, lung, lymph nodes, thymus, bone, ligaments, tendons, skeletal muscle, dermis, and periosteum. Thus, STRO-1+ multipotent cells can differentiate into a number of cell types, including, but not limited to, adipose tissue, bone tissue, cartilage tissue, elastic tissue, and fibrous connective tissue. The particular lineage commitment and differentiation pathway that these cells enter are determined by various influences from mechanical influences and / or intrinsic bioactive factors, such as growth factors, cytokines, and / or local microenvironmental conditions established by the host tissue. In one embodiment, STRO-1+ multipotent cells are non-hematopoietic progenitor cells that divide to give rise to daughter cells, either stem cells or progenitor cells, at a time when they will irreversibly differentiate to give rise to phenotypic cells.

[0098] In one embodiment, STRO-1+ cells are enriched from a sample obtained from a subject, e.g., the subject to be treated or a related or unrelated subject (whether of the same or different species). The terms "enriched" and "enrichment" or variations thereof are used herein to describe a population of cells in which the proportion of one particular cell type or the proportion of a number of particular cell types is increased compared to an untreated population of cells (e.g., cells in their native environment). In one embodiment, an enriched population of STRO-1+ cells comprises at least about 0.1%, 0.5%, 1%, 2%, 5%, 10%, 15%, 20%, 25%, 30%, 50%, 75%, 85%, 95%, or 99% STRO-1+ cells. In this context, the term "enriched population of STRO-1+ cells" will be construed as explicitly supporting the term "a population of cells comprising X% STRO-1+ cells" (where X% is a percentage as described herein). STRO-1+ cells may, in some instances, form clonogenic colonies, e.g., CFU-F (fibroblasts), or a subset thereof (e.g., 50% or 60% or 70% or 80% or 90% or 95%) may have this activity.

[0099] In one embodiment, stem cells expressing high levels of Ang1 are enriched from a cell preparation comprising STRO-1+ cells in a selectable format. In this context, the term "selectable format" will be understood to mean that the cells express a marker (e.g., a cell surface marker) that allows for the selection of STRO-1+ cells. The marker can be, but need not be, STRO-1. For example, as described and / or exemplified herein, cells (e.g., MPCs) that express STRO-2 and / or STRO-3 (TNAP) and / or STRO-4 and / or VCAM-1 and / or CD146 and / or 3G5 also express STRO-1 (and STRO-1 bright(It may also be that the cell is selected based on expression of STRO-1+). Thus, indicating that a cell is STRO-1+ does not imply that the cell has been selected due to expression of STRO-1. In one embodiment, the cell is selected based on at least expression of STRO-3, e.g., STRO-3+(TNAP+). In one embodiment, the cell is selected based on at least expression of STRO-4, e.g., STRO-4+.

[0100] Reference to the selection of a cell or population does not necessarily require selection from a particular tissue source. As described herein, STRO-1+ cells can be selected, isolated or enriched from a wide variety of sources. Accordingly, in some instances, these terms provide support for selection from either a tissue containing STRO-1+ cells (e.g., MPCs), or a vascularized tissue, or a tissue containing pericytes (e.g., STRO-1+ pericytes), or from any one or more of the tissues described herein.

[0101] In one embodiment, stem cells expressing high levels of Ang1 express one or more markers individually or collectively selected from the group consisting of STRO-1+, TNAP+, VCAM-1+, THY-1+, STRO-2+, STRO-4+(HSP-90β), CD45+, CD146+, 3G5+, CC9 or any combination thereof.

[0102] "Individually" means that the disclosure encompasses each listed marker or group of markers individually, and that the appended claims may define each such marker or group of markers individually and separably from one another, even though each marker or group of markers may not be individually listed herein.

[0103] "Collectively" means that the disclosure encompasses any number or combination of the listed markers or peptides, and that notwithstanding that such numbers or combinations of markers or markers may not be specifically recited herein, the appended claims may define such combinations or sub-combinations individually and separably from any other combinations of markers or markers.

[0104] In one embodiment, STRO-1+ cells are bright (syn.STRO-1 bri In one embodiment, STRO-1 bri STRO-1 cells dim or STRO-1 intermediate are preferentially enriched relative to cells.

[0105] In one embodiment, STRO-1 bright The cells may further be one or more of TNAP+, VCAM-1+, THY-1+, STRO-2+, STRO-4+ (HSP-90β) and / or CD146+. For example, the cells may be selected for one or more of the aforementioned markers and / or may show expression of one or more of these markers. In this regard, cells shown to express a marker need not be specifically tested, and conversely, previously enriched or isolated cells may be tested and subsequently used. Also, it may be reasonably assumed that the isolated or enriched cells express the same markers.

[0106] In one embodiment, STRO-1 bright is isolated by immune selection. In one embodiment, STRO-1 brightThe cells are isolated by immunoselection of cells expressing TNAP. The term "TNAP" as used herein is intended to encompass all isoforms of tissue non-specific alkaline phosphatase. For example, the term encompasses the liver isoform (LAP), the bone isoform (BAP) and the kidney isoform (KAP). In one embodiment, TNAP is BAP. In one embodiment, TNAP as used herein refers to a molecule capable of binding to STRO-3 antibody produced by a hybridoma cell line based on the deposit under the provisions of the Budapest Treaty, accession number PTA-7282, deposited with the ATCC on December 19, 2005.

[0107] In one embodiment, the mesenchymal progenitor or stem cells are CD29+, CD54+, CD73+, CD90+, CD102+, CD105+, CD106+, CD166+, MHC1+ mesenchymal stem cells (e.g., remestemcel-L).

[0108] In one embodiment, the mesenchymal precursor cells are perivascular mesenchymal precursor cells as defined in WO2004 / 85630. For example, the mesenchymal precursor cells are cells that express perivascular cell markers, for example, STRO-1+ or STRO-1 bright and / or 3G5+, etc. In one embodiment, the cells are or were formerly progeny of cells isolated from vascular tissue or an organ or portion thereof.

[0109] When referring to cells that are "positive" for a given marker, it can be either low (lo or dim) or high (bright, bri) levels of that marker, depending on the extent to which the marker is present on the cell surface, and the term refers to the fluorescence intensity or other marker used in the cell sorting process. The distinction between lo (or dim or dull) and bri will be understood in the context of the marker used for the particular cell population being sorted. When referring to cells that are "negative" for a given marker, it does not mean that the marker is not expressed at all by the cell. This term means that the marker is expressed at a relatively very low level by the cell, and when detectably labeled, produces a very low signal or is not detected above background levels. For example, the level is detected using an isotype control antibody.

[0110] As used herein, the term "bright" refers to a marker on the cell surface that produces a relatively high signal when detectably labeled. Without wishing to be limited by theory, it is proposed that "bright" cells express more of the target marker protein (e.g., the antigen recognized by STRO-1) than other cells in the sample. For example, STRO-1 bri The cells were non-bright cells (STRO-1 dull / dim ) yields a greater fluorescent signal when labeled with FITC-conjugated STRO-1 antibody as measured by fluorescence activated cell sorting (FACS) analysis. In one embodiment, the "bright" cells comprise at least about 0.1% of the most brightly labeled bone marrow mononuclear cells in the starting sample. In other embodiments, the "bright" cells comprise at least about 0.1%, at least about 0.5%, at least about 1%, at least about 1.5%, or at least about 2% of the most brightly labeled bone marrow mononuclear cells in the starting sample. In one embodiment, the "bright" cells comprise at least about 0.1%, at least about 0.5%, at least about 1%, at least about 1.5%, or at least about 2% of the most brightly labeled bone marrow mononuclear cells in the starting sample. brightThe cells have 2 log magnitude higher expression of STRO-1 surface expression compared to "background", i.e., cells that are STRO-1-. dim and / or STRO-1 intermediate The cells have less than 2 log magnitude of STRO-1 surface expression, typically less than about 1 log or below "background."

[0111] In one embodiment, a significant proportion of STRO-1+ multilineage cells can differentiate into at least two different germline cell lines. Non-limiting examples of lineages to which multilineage cells can be committed include bone progenitor cells; hepatocyte progenitor cells that are multipotent for bile duct epithelial cells and hepatocytes; neural-restricted cells that can give rise to glial progenitor cells that progress to oligodendrocytes and astrocytes; neural progenitor cells that progress to neurons; cardiac muscle and cardiac muscle cell progenitors, glucose-responsive insulin-secreting pancreatic beta cell lines. Other lineages include, but are not limited to, odontoblasts, dentin-producing cells and chondrocytes, as well as the following progenitor cells: retinal pigment epithelial cells, fibroblasts, skin cells such as keratinocytes, dendritic cells, hair follicle cells, renal tubular epithelial cells, smooth and skeletal muscle cells, testicular progenitor cells, vascular endothelial cells, tendons, ligaments, cartilage, adipocytes, fibroblasts, bone marrow stroma, cardiac muscle, smooth muscle, skeletal muscle, pericytes, vascular cells, epithelial cells, glial cells, neurons, astrocytes and oligodendrocytes.

[0112] In another embodiment, STRO-1+ cells are unable to give rise to hematopoietic cells in culture.

[0113] In one embodiment, the stem cells described herein are mesenchymal stem cells. Mesenchymal stem cells (MSCs) may be of homogenous composition or may be a mixed cell population enriched in MSCs. Homogeneous mesenchymal stem cell compositions may be obtained by culturing adherent bone marrow or periosteal cells, and mesenchymal stem cells may be identified by specific cell surface markers identified using specific monoclonal antibodies. Methods for obtaining cell populations enriched in mesenchymal stem cells are described, for example, in U.S. Patent No. 5,486,359. Alternative sources of mesenchymal stem cells include, but are not limited to, blood, skin, umbilical cord blood, muscle, fat, bone, and perichondrium.

[0114] The recognition, selection and purification of stem cells bearing the above-mentioned cell surface markers can be achieved by a number of different methods, such as by applying a binding agent for the marker of interest, followed by isolation of those cells that show either high levels of binding, or low levels of binding, or no binding at all.

[0115] For example, the binding agent can include an antibody, such as a monoclonal antibody or an antibody-based molecule.

[0116] Antibodies and other binding molecules can be used in a variety of techniques to select and purify stem cells expressing specific cell surface markers.

[0117] Techniques for selection and purification may include, but are not limited to, magnetic separation using antibody-coated magnetic beads, affinity chromatography, and "panning" with antibodies bound to solid matrices, fluorescence-activated cell sorting (FACS).

[0118] Stem cells expressing high levels of Ang1 that express specific markers may be selected or purified from a cell population via positive immunoselection. For example, mesenchymal progenitor cells can be isolated and enriched from a cell population based on cell surface expression of the STRO-1 antibody (see, e.g., Gronthos and Simmons 1995).

[0119] According to the present disclosure, isolated stem cells can be expanded in vitro by culturing. As will be appreciated by one of skill in the art, stem cells can be expanded in vitro by cryopreservation, thawing, and subsequent culturing. In one example, stem cells are seeded in growth medium and cultured at 37° C., 20% O 2 After that, the growth medium was replaced and the cells were incubated at 37°C, 5% O 2 Culture the cells at RT for an additional 68-72 h.

[0120] In one embodiment, isolated stem cells are cultured at 50,000 cells / cm in serum-supplemented growth medium. 2 and incubated at 37 °C and 20% O 2 The cells were allowed to attach to the culture vessel overnight at 4°C. The growth medium was then replaced with cartilage basal medium (CBM; Lonza, Walkersville, MD) supplemented with 0.5% bovine serum albumin (BSA) and incubated at 37°C, 5% O. 2 Culture the cells at RT for an additional 68-72 h.

[0121] Various other methods of primary stem cell culture are well known in the art. For example, primary stem cell culture can be performed using the method described in Gronthos and Simmons 1995.

[0122] Cultured stem cells are phenotypically distinct from cells in vivo, for example, they can express CD44.

[0123] In one embodiment, cultured stem cells are biologically distinct from in vivo cells and have a faster renewal rate.

[0124] The cultured stem cells may be cryopreserved prior to administration to a subject, for example, stem cells expressing high levels of Ang1 are cryopreserved prior to administration to a subject.

[0125] Genetically unmodified cells As used herein, the term "genetically unmodified" refers to cells that have not been modified by transfection with a nucleic acid that expresses or encodes Ang1. For the avoidance of doubt, stem cells transfected with a nucleic acid encoding Ang1 in the context of the present disclosure are considered to be genetically modified. In the context of the present disclosure, "genetically unmodified" cells naturally express Ang1 to some degree.

[0126] Ang1 and / or VEGF expression Stem cells expressing high levels of Ang1 were genetically unaltered and were at least 0.1 μg / 10 6 However, in various embodiments, stem cells expressing high levels of Ang1 are at least 0.2 μg / 10 6 cells, 0.3μg / 10 6 cells, 0.4μg / 10 6 cells, 0.5μg / 10 6 cells, 0.6μg / 10 6 cells, 0.7μg / 10 6 cells, 0.8μg / 10 6 cells, 0.9μg / 10 6 cells, 1μg / 10 6 cells, 1.1μg / 10 6 cells, 1.2μg / 10 6 cells, 1.3μg / 10 6 cells, 1.4μg / 10 6 cells, 1.5μg / 10 6 It is envisaged that Ang1 may be expressed in amounts of cells.

[0127] In one embodiment, the stem cells expressing high levels of Ang1 are at least 0.2 μg / 10 6 Cells ~ approx. 1.5μg / 10 6 The amount of cells expressing Ang1.

[0128] In one embodiment, the stem cells expressing high levels of Ang1 are at least 0.3 μg / 10 6 Cells ~ approx. 1.4μg / 10 6The amount of cells expressing Ang1.

[0129] In one embodiment, the stem cells expressing high levels of Ang1 are at least 0.4 μg / 10 6 Cells ~ approx. 1.3μg / 10 6 The amount of cells expressing Ang1.

[0130] In one embodiment, the stem cells expressing high levels of Ang1 are at least 0.5 μg / 10 6 Cells ~ approx. 1.2μg / 10 6 The amount of cells expressing Ang1.

[0131] In one embodiment, the stem cells expressing high levels of Ang1 are at least 0.55 μg / 10 6 Cells ~ approx. 1.1μg / 10 6 The amount of cells expressing Ang1.

[0132] In one embodiment, the stem cells expressing high levels of Ang1 are at least 0.6 μg / 10 6 Cells ~ approx. 1.0μg / 10 6 The amount of cells expressing Ang1.

[0133] In one embodiment, the stem cells expressing high levels of Ang1 are at least 0.65 μg / 10 6 Cells ~ approx. 0.9μg / 10 6 The amount of cells expressing Ang1.

[0134] In one embodiment, the stem cells expressing high levels of Ang1 are at least 0.7 μg / 10 6 Cells ~ approx. 0.8μg / 10 6 The amount of cells expressing Ang1.

[0135] In another embodiment, the genetically unmodified stem cells expressing high levels of Ang1 are administered at a concentration of about 0.01 μg / 10 6 They express VEGF in subcellular amounts.

[0136] In another embodiment, the genetically unmodified stem cells expressing high levels of Ang1 are administered at a concentration of about 0.05 μg / 10 6 However, in various embodiments, stem cells expressing high levels of Ang1 express VEGF in amounts less than about 0.05 μg / 10 6 cells, 0.04μg / 10 6 cells, 0.03μg / 10 6 cells, 0.02μg / 10 6 cells, 0.01μg / 10 6 cells, 0.009μg / 10 6 cells, 0.008μg / 10 6 cells, 0.007μg / 10 6 cells, 0.006μg / 10 6 cells, 0.005μg / 10 6 cells, 0.004μg / 10 6 cells, 0.003μg / 10 6 cells, 0.002μg / 10 6 cells, 0.001μg / 10 6 It is envisioned that VEGF may be expressed in subcellular amounts.

[0137] In one embodiment, the stem cells expressing high levels of Ang1 are at least 0.001 μg / 10 6 Cells ~ approx. 0.1μg / 10 6 Amount of cells expressing VEGF.

[0138] In one embodiment, the stem cells expressing high levels of Ang1 are at least 0.0025 μg / 10 6 Cells ~ approx. 0.09μg / 10 6 Amount of cells expressing VEGF.

[0139] In one embodiment, the stem cells expressing high levels of Ang1 have a concentration of at least 0.0075 μg / 10 6 Cells ~ approx. 0.08μg / 10 6 Amount of cells expressing VEGF.

[0140] In one embodiment, the stem cells expressing high levels of Ang1 are at least 0.01 μg / 10 6Cells ~ approx. 0.07μg / 10 6 Amount of cells expressing VEGF.

[0141] In one embodiment, the stem cells expressing high levels of Ang1 are at least 0.02 μg / 10 6 Cells ~ approx. 0.06μg / 10 6 Amount of cells expressing VEGF.

[0142] In one embodiment, the stem cells expressing high levels of Ang1 are at least 0.02 μg / 10 6 Cells ~ approx. 0.05μg / 10 6 Amount of cells expressing VEGF.

[0143] The amount of cellular Ang1 and / or VEGF expressed in the composition or stem cell culture may be determined by methods well known to those of skill in the art. Such methods include, but are not limited to, quantitative assays such as, for example, quantitative ELISA assays. However, it should be understood that the scope of the present disclosure is not limited to any particular method for determining the amount or level of Ang1 or VEGF expressed in stem cells expressing high levels of Ang1.

[0144] In one example, the levels of Ang1 or VEGF expressed by the composition or stem cell culture are determined by an ELISA assay. In such an assay, cell lysates from stem cell cultures are added to the wells of an ELISA plate. The wells may be coated with a primary antibody, either monoclonal or polyclonal antibody(ies), against Ang1 or VEGF. The wells are then washed and then contacted with a secondary antibody, either monoclonal or polyclonal antibody(ies), against the primary antibody. The secondary antibody is conjugated to a suitable enzyme, for example, horseradish peroxidase. The wells may then be incubated and washed after a period of incubation. The wells are then contacted with a suitable substrate for the enzyme conjugated to the secondary antibody, such as one or more chromogens. Chromogens that can be used include, but are not limited to, hydrogen peroxide and tetramethylbenzidine. After the substrate(s) are added, the wells are incubated for a suitable period of time. Once incubation is complete, a "stop" solution is added to the wells to stop the reaction of the substrate(s) with the enzyme. The optical density (OD) of the sample is then measured and correlated with the optical density of samples containing known amounts of Ang1 or VEGF to determine the amount of Ang1 or VEGF expressed by the stem cell culture being tested.

[0145] In another aspect, genetically unaltered stem cells expressing high levels of Ang1 express Ang1:VEGF in a ratio of at least about 2: 1. However, in various embodiments, it is contemplated that stem cells expressing high levels of Ang1 may express Ang1:VEGF in a ratio of at least about 10:1, 15:1, 20:1, 21:1, 22:1, 23:1, 24:1, 25:1, 26:1, 27:1, 28:1, 29:1, 30:1, 31:1, 32:1, 33:1, 34:1, 35:1, 50:1.

[0146] Methods for determining the expression ratio of Ang1:VEGF will be apparent to those skilled in the art. In an embodiment of the method for determining the expression ratio of Ang1 and VEGF, the expression levels of Ang1 and VEGF are quantified via quantitative ELISA as described above. In such an embodiment, after the levels of Ang1 and VEGF are quantified, the ratio based on the quantified levels of Ang1 and VEGF can be expressed as follows: (Level of Ang1 / Level of VEGF)=Ratio of Ang1:VEGF.

[0147] cell composition In one embodiment of the present disclosure, the stem cells are administered in the form of a composition, hi one embodiment, such a composition includes a pharma- ceutically acceptable carrier and / or excipient.

[0148] The terms "carrier" and "excipient" refer to a composition of matter conventionally used in the art to store, administer, and / or facilitate the biological activity of an active compound (see, e.g., Remington's Pharmaceutical Sciences, 16th ed., Mac Publishing Company (1980)). A carrier may also reduce any undesirable side effects of an active compound. A suitable carrier is, for example, stable and, for example, incapable of reacting with other components in the carrier. In one embodiment, a carrier causes no significant local or systemic adverse effects in a recipient at the dosages and concentrations used therapeutically.

[0149] Suitable carriers for the present disclosure include those that are conventionally used.For example, water, saline, aqueous dextrose, lactose, Ringer's solution, buffer, hyaluronic acid and glycols are exemplary liquid carriers, especially for liquid formulations (when isotonic).Suitable pharmaceutical carriers and excipients include starch, cellulose, glucose, lactose, sucrose, gelatin, malt, rice, flour, chalk, silica gel, magnesium stearate, sodium stearate, glycerol monostearate, sodium chloride, glycerin, propylene glycol, water, ethanol, etc.

[0150] In another example, the carrier is, for example, a media composition that the cells are grown or suspended in. For example, the media composition does not induce any adverse effects in the subject to which it is administered.

[0151] Exemplary carriers and excipients do not adversely affect cell viability and / or the ability of the cells to reduce, prevent or delay metabolic syndrome and / or obesity.

[0152] In one embodiment, the carrier or excipient provides buffering, for example, to maintain cells and / or soluble factors at an appropriate pH, thereby exerting biological activity. For example, the carrier or excipient is phosphate buffered saline (PBS). PBS is an attractive carrier or excipient because it interacts minimally with cells and factors, allowing for rapid release of cells and factors, such that the compositions of the present disclosure may be prepared as a liquid for direct application (e.g., by injection) into the bloodstream, or into tissues or into areas surrounding or adjacent to tissues.

[0153] Stem cells and / or their progeny may also be incorporated or embedded in scaffolds that are recipient-compatible and degrade into products that are not harmful to the recipient. These scaffolds provide support and protection for the cells that are transplanted into the recipient subject. Natural and / or synthetic biodegradable scaffolds are examples of such scaffolds.

[0154] A variety of different scaffolds may be successfully used in the practice of the present invention. Exemplary scaffolds include, but are not limited to, biological, degradable scaffolds. Natural biodegradable scaffolds include collagen, fibronectin and laminin scaffolds. A suitable synthetic material for a cell transplant scaffold should be able to support a wide range of cell growth and cell function. Such scaffolds may be resorbable. Suitable scaffolds include polyglycolic acid scaffolds, such as those described in Vacanti et al. JP Surg. 23:3-9 1988; Cima et al. Biotechnol. Bioeng. 38:145 1991; Vacanti et al. Plast. Reconstr. Surg. 88:753-9 1991, or synthetic polymers such as polyanhydrides, polyorthoesters, and polylactic acids.

[0155] In another embodiment, the cells may be administered in a gel-like scaffold (such as Gelfoam from the Upjohn Company).

[0156] The cell compositions described herein may be administered alone or as a mixture with other cells. Different types of cells may be mixed with the compositions of the present disclosure immediately or shortly before administration, or may be co-cultured together for a period of time before administration.

[0157] In one embodiment, the composition comprises an effective amount, or a therapeutically or prophylactically effective amount, of cells. For example, the composition comprises about 1×10 5 Approximately 1×10 stem cell cells / kg with high Ang1 levels 7 stem cells / kg, or approximately 1 × 10 6 Approximately 5×10 stem cells / kg with high Ang1 levels 6 Contains stem cells / kg.

[0158] The exact dose of stem cells to be administered will depend on a variety of factors, including, but not limited to, the age, weight, and sex of the patient, the disease or disorder being treated, and its extent and severity.

[0159] In one embodiment, a low dose of cells is administered to a subject. An exemplary dose is about 0.1×10 cells per kg. 4 ~about 0.5×10 6 Between 10 and 10 cells, for example, about 0.1 × 10 cells per kg 5 ~about 0.5×10 6 Between 10 and 10 cells, for example, about 0.5 × 10 cells per kg 5 ~about 0.5×10 6 Between 10 and 10 cells, for example, about 0.1 × 10 cells per kg 6 ~about 0.5×10 6 Between 10 and 10 cells, for example, about 0.2 × 10 cells per kg 6 pcs or 0.3×10 6 pcs or 0.4×10 6 Contains cells.

[0160] For example, about 0.1 x 10 per kg 6 , about 0.2×10 6 , about 0.3×10 6 , about 0.4×10 6 , about 0.5×10 6 The cells can be administered to a subject.

[0161] In another embodiment, about 0.6×10 per kg 6 , about 0.7×10 6 , about 0.8×10 6 , about 0.9×10 6 , about 1.0×10 6 , about 1.1×10 6 , about 1.2×10 6 , about 1.3×10 6 , about 1.4×10 6 The cells can be administered to a subject.

[0162] In one embodiment, a high dose of cells is administered to a subject. An exemplary dose is at least about 1.5×10 6 For example, a high dose would be approximately 1.5 x 10 cells / kg. 6 ~about 6×10 6 between 1.5 x 10 cells / kg, for example about 1.5 x 10 6~Approx. 5×10 6 Between 1.5 x 10 cells / kg, for example, about 1.5 x 10 6 ~Approx. 4×10 6 Between 1.5 x 10 cells / kg, for example, about 1.5 x 10 6 ~Approx. 3×10 6 For example, a high dose would include approximately 1.5 x 10 cells / kg. 6 pcs or approx. 2 x 10 6 For example, a high dose would be approximately 1.5 x 10 cells / kg. 6 For example, a high dose would be approximately 2 × 10 cells / kg. 6 For example, a high dose would be approximately 3×10 cells / kg. 6 Contains cells / kg.

[0163] In another embodiment, about 1.5×10 per kg 6 , about 1.6×10 6 , about 1.7×10 6 , about 1.8×10 6 , about 1.9×10 6 , about 2.0×10 6 , about 2.1×10 6 , about 2.2×10 6 , about 2.3×10 6 , about 2.4×10 6 , about 2.5×10 6 , about 2.6×10 6 , about 2.7×10 6 , about 2.8×10 6 , about 2.9×10 6 , about 3.0×10 6 , about 3.1×10 6 , about 3.2×10 6 , about 3.3×10 6 , about 3.4×10 6 , about 3.5×10 6 , about 3.6×10 6 , about 3.7×10 6 , about 3.8×10 6 , about 3.9×10 6 , about 4.0×10 6 The cells are administered to the subject.

[0164] In another embodiment, about 1.0×10 8, about 1.1×10 8 , about 1.2×10 8 , about 1.3×10 8 , about 1.4×10 8 , about 1.5×10 8 , about 1.6×10 8 , about 1.7×10 8 , about 1.8×10 8 , about 1.9×10 8 , about 2.0×10 8 , about 2.1×10 8 , about 2.2×10 8 , about 2.3×10 8 , about 2.4×10 8 , about 2.5×10 8 , about 2.6×10 8 , about 2.7×10 8 , about 2.8×10 8 , about 2.9×10 8 cells, approximately 3.0 x 10 8 , about 3.1×10 8 , about 3.2×10 8 , about 3.3×10 8 , about 3.4×10 8 , about 3.5×10 8 , about 3.6×10 8 , about 3.7×10 8 , about 3.8×10 8 , about 3.9×10 8 , about 4.0×10 8 The cells are administered to the subject.

[0165] The mesenchymal precursor or stem cells may comprise at least about 5% of the cell population of the composition. In other examples, the mesenchymal precursor or stem cells may comprise at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95% of the cell population of the composition.

[0166] Stem cells expressing CD44 may comprise at least about 5% of the cell population of the composition. In other examples, stem cells expressing CD44 may comprise at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 99%, at least about 100% of the cell population of the composition.

[0167] In some embodiments, the cells are contained within a chamber that does not allow the cells to exit into the circulation of a subject, but allows factors secreted by the cells to enter the circulation. In such methods, soluble factors may be administered to a subject by allowing the cells to secrete the factors into the circulation of the subject. Such chambers may be simultaneously implanted at a site in a subject to increase local levels of soluble factors.

[0168] The stem cells can be administered systemically, for example, by intravenous, intraarterial, or intraperitoneal administration.

[0169] The mesenchymal progenitor or stem cells can also be administered by intranasal, intramuscular, intraarticular or intracardiac administration.

[0170] For example, mesenchymal progenitor or stem cells can be administered directly to a painful or swollen joint.

[0171] In another embodiment, stem cells expressing high levels of Ang1 are administered by intracoronary infusion. For example, mesenchymal progenitor or stem cells can be administered into the left anterior descending (LAD) artery.

[0172] In another embodiment, stem cells expressing high levels of Ang1 are administered by intrarenal infusion.

[0173] In one example, stem cells expressing high levels of Ang1 may be administered in a single dose.

[0174] In some embodiments, stem cells expressing high levels of Ang1 can be administered in multiple doses, for example, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10 doses.

[0175] Compositions containing stem cells expressing high levels of Ang1 may be cryopreserved. Cryopreservation of stem cells can be performed using slow cooling methods or "rapid" freezing procedures known in the art. In one embodiment, the cryopreservation method maintains a similar phenotype, cell surface markers, and growth rate of the cryopreserved cells compared to non-frozen cells.

[0176] The cryopreserved composition may comprise a cryopreservation solution, the pH of which is typically about 6.5-8.

[0177] In one embodiment, the pH of the cryopreservation medium is about 7.4.

[0178] The cryopreservation medium may, for example, comprise Plasmalyte A®, a sterile, non-pyrogenic, isotonic solution. 100 mL of Plasmalyte A® contains 526 mg of Sodium Chloride, USP (NaCl), 502 mg of Sodium Gluconate (C 6 H 11 NaO 7 ), 368 mg of Sodium Acetate Trihydrate, USP(C 2 H 3 NaO 2 3H 2 0), 37 mg of Potassium Chloride, USP (KCl), and 30 mg of Magnesium Chloride, USP (MgCl 2 6H 2 Contains no antibacterial agents. The pH is adjusted with sodium hydroxide. The pH is 7.4 (6.5-8.0).

[0179] To facilitate freezing, a cryoprotectant, such as dimethyl sulfoxide (DMSO), is usually added to the cryopreservation solution. Ideally, the cryoprotectant should be non-toxic for the cells and the patient, chemically inert, non-antigenic, provide high survival rates after thawing, and allow transplantation without washing. However, DMSO, the most commonly used cryoprotectant, exhibits some cytotoxicity. Hydroxyethyl starch (HES) can be used as an alternative or in combination with DMSO to reduce the cytotoxicity of the cryopreservation solution.

[0180] The cryopreservation solution may include one or more of DMSO, hydroxyethyl starch, human serum components, and other protein bulking agents. In one embodiment, the cryopreservation solution includes about 5% human serum albumin (HSA) and about 10% DMSO. The cryopreservation solution may further include one or more of methylcellulose, polyvinylpyrrolidone (PVP), and trehalose.

[0181] The cryopreserved composition may be thawed and administered directly to a subject, or the cryopreserved composition may be thawed and the mesenchymal precursors or stem cells resuspended in a replacement solution prior to administration.

[0182] The stem cells expressing high levels of Ang1 are administered to the animal in an amount effective to treat a disease or disorder in the animal. The animal can be a mammal, and the mammal can be a primate, including humans and non-human primates.

[0183] In one embodiment, stem cells expressing high levels of Ang1 are administered to a human.

[0184] In one embodiment, stem cells expressing high levels of Ang1 are administered to a human.

[0185] In one example, stem cells expressing high levels of Ang1 are administered to a subject suffering from diabetes.

[0186] In one example, stem cells expressing high levels of Ang1 are administered to a subject suffering from type II diabetes.

[0187] In one embodiment, stem cells expressing high levels of Ang1 are administered to a subject suffering from type II diabetes and having a baseline HbA1c level of greater than about 7%. For example, stem cells expressing high levels of Ang1 can be administered to a subject suffering from type II diabetes and having a baseline HbA1c level of greater than about 7.1%, about 7.2%, about 7.3%, about 7.4%, about 7.5%, about 7.6%, about 7.7%, about 7.8%, about 7.9%, about 8.0%, about 8.1%, about 8.2%, about 8.3%, about 8.4%, about 8.5%, about 8.6%, about 8.7%, about 8.8%, about 8.9%, about 9.0%.

[0188] In one example, stem cells expressing high levels of Ang1 can be administered to a subject suffering from type II diabetes and having a baseline HbA1c level of about 8.0% or greater.

[0189] Methods for determining HbA1c values ​​(percentage of total hemoglobin) will be clear to those skilled in the art. Examples of methods used to determine HbA1c values ​​(percentage of total hemoglobin) include high performance liquid chromatography (HPLC) or immunoassay. Those skilled in the art will also recognize that HbA1c values ​​can be expressed in other values, for example, mmol / mol.

[0190] In one example, the subject's HbA1c level is determined by HPLC.

[0191] In one example, the subject's HbA1c level is determined by immunoassay.

[0192] In one embodiment, stem cells expressing high levels of Ang1 are administered to a patient suffering from type II diabetes, where the subject's glucose levels are poorly controlled.

[0193] In one embodiment, stem cells expressing high levels of Ang1 are administered to a subject suffering from type II diabetes, and the subject's glucose levels are not adequately controlled by metformin or metformin and another oral therapeutic agent.

[0194] In one example, stem cells expressing high levels of Ang1 are administered to a subject suffering from chronic kidney disease.

[0195] In one example, stem cells expressing high levels of Ang1 are administered to a subject suffering from diabetic nephropathy.

[0196] In one embodiment, stem cells expressing high levels of Ang1 are administered to a subject suffering from type II diabetes and chronic kidney disease.

[0197] In one embodiment, stem cells expressing high levels of Ang1 are administered to a subject suffering from type II diabetes and diabetic nephropathy.

[0198] Estimated glomerular filtration rate (eGFR) is used to screen and detect early kidney damage and monitor kidney status.

[0199] In one embodiment, stem cells expressing high levels of Ang1 are associated with a baseline eGFR of about 35 ml / min / 1.73 m 2 , about 34ml / min / 1.73m 2 , about 44ml / min / 1.73m 2 , about 32ml / min / 1.73m 2 , about 31ml / min / 1.73m 2 , about 30ml / min / 1.73m 2 , approx. 29ml / min / 1.73m 2 , about 28ml / min / 1.73m 2 , about 27ml / min / 1.73m 2 , about 26ml / min / 1.73m 2 , about 25ml / min / 1.73m 2 It is administered to more than 10 subjects.

[0200] In one example, stem cells expressing high levels of Ang1 are administered to a subject with renal function stage 3A, 3B, 4 or 5.

[0201] In one example, stem cells expressing high levels of Ang1 are administered to a subject with renal function stage 3B.

[0202] In one embodiment, stem cells expressing high levels of Ang1 are administered to a subject.

[0203] In one embodiment, stem cells expressing high levels of Ang1 are associated with a baseline eGFR of 30 ml / min / 1.73 m 2 It is administered to a subject who meets or exceeds the above criteria.

[0204] Methods for estimating GFR will be apparent to those skilled in the art, but examples are provided below: eGFR can be calculated using creatinine and / or cystatin C levels.

[0205] For example, the eGFR can be calculated using the following MDRD formula: GFR (mL / min / 1.73m 2 )=175×(S cr ) -1.154 ×(age) -0.203 × (0.742 for women) × (1.212 for African-Americans)

[0206] In another embodiment, eGFR may be calculated using the CKD-EPI formula (Levey et al., Ann Intern. Med. 150(9), 604-12, 2009). GFR = 141 × min(S cr / κ, 1) α ×max(S cr / κ, 1) -1.209 ×0.993 年齢 × 1.018 [for women] × 1.159 [for black people] During the ceremony, S cr is serum creatinine (mg / dL), Kappa was 0.7 in women and 0.9 in men; Alpha was −0.329 for women and −0.411 for men; min is S cr / κ is the minimum or 1, max is S cr / κ is the maximum value or 1.

[0207] Additionally, eGFR can be serially calculated and monitored over time to determine if the eGFR is declining or improving. The eGFR can be compared between a control group and a treatment group to determine if the decline in eGFR is inhibited in the treatment group.

[0208] In one example, stem cells expressing high levels of Ang1 are administered to a subject suffering from arthritis.

[0209] In one example, stem cells expressing high levels of Ang1 are administered to a subject suffering from rheumatoid arthritis.

[0210] In one embodiment, stem cells expressing high levels of Ang1 are administered to a subject suffering from rheumatoid arthritis who is classified as an inadequate anti-TNFα responder.

[0211] In one embodiment, stem cells expressing high levels of Ang1 are administered to a subject suffering from rheumatoid arthritis who has failed biological therapy for rheumatoid arthritis.

[0212] In one example, stem cells expressing high levels of Ang1 are administered to a subject suffering from rheumatoid arthritis who has failed two biologic therapies for rheumatoid arthritis.

[0213] In one example, stem cells expressing high levels of Ang1 are administered to a subject suffering from rheumatoid arthritis who has failed three biological therapies for rheumatoid arthritis.

[0214] Inhibition of TNF-alpha, IL-6, and IL-17 TNF-alpha, IL-6 and IL-17 are chemical messengers known as cytokines. These molecules are released from cells in response to various signals. In the context of the present invention, the term "inhibit" or "inhibiting" refers to the reduction or suppression of a measurable level of a substance such as a protein (e.g., a cytokine) or a process (e.g., cell differentiation or cell polarization).

[0215] Thus, in one embodiment, it is contemplated that administration of a cell composition comprising stem cells expressing high levels of Ang1 will reduce measurable levels of TNF-alpha, IL-17 and / or IL-6 in a subject, in this embodiment, TNF-alpha, IL-17 and / or IL-6 release from the cells is inhibited.

[0216] In one embodiment, the disclosure relates to a method for monitoring a subject's response to administration of stem cells expressing high levels of Ang1. In this embodiment, following administration of stem cells expressing high levels of Ang1, the levels or pro-inflammatory and / or anti-inflammatory markers such as cytokines can be monitored over a period of time.

[0217] In one example, the disclosure relates to a method for monitoring a subject's response following administration of stem cells expressing high levels of Ang1, the method comprising assessing inflammatory and / or anti-inflammatory marker levels in a sample, such as a whole blood sample, obtained from a subject administered stem cells expressing high levels of Ang1, and determining whether the subject has responded to the administration of stem cells expressing high levels of Ang1 based on the pro-inflammatory and / or anti-inflammatory marker levels.

[0218] In one embodiment, an increase in the level of an anti-inflammatory marker and / or a decrease in the level of an inflammatory marker indicates that the subject has responded to administration of stem cells.

[0219] In one embodiment, the inflammatory and / or anti-inflammatory markers are cells or cell populations.

[0220] In one embodiment, an increase in the number of anti-inflammatory cells and / or a decrease in the number of inflammatory cells indicates that the subject has responded to administration of stem cells.

[0221] In one embodiment, the anti-inflammatory cells are Th2 cells, Treg cells and / or M2-type macrophages.

[0222] In one embodiment, the inflammatory cells are Th17 cells and / or M1 type macrophages.

[0223] A variety of assays are available to one of skill in the art that can be used to determine whether the number of inflammatory cells has been reduced and / or the number of anti-inflammatory cells has been increased.

[0224] For example, cell populations isolated from whole blood samples can be assessed for expression of cell surface markers using flow cytometry-based techniques such as fluorescence activated cell sorting (FACS).

[0225] In one example, CD14+ mononuclear cells can be purified from a whole blood sample obtained from a subject administered stem cells expressing high levels of Ang1. The mononuclear cells can be assessed for CD16, CD163, and CD206 expression to identify the ratio of M1 and M2 macrophages. Multiple samples can be assessed over time to determine whether the number of M1 macrophages has decreased or is decreasing, and / or the level of M2 macrophages has increased or is increasing. In one example, the number of M1 and / or M2 macrophages is assessed relative to the number of M1 and / or M2 macrophages in a sample obtained from the subject prior to administration of stem cells (e.g., a baseline or pre-treatment reference sample).

[0226] In another embodiment, the inflammatory and / or anti-inflammatory markers assessed are cytokines.

[0227] In one embodiment, the inflammatory marker is TNF-alpha, IL-17 and / or IL-6.

[0228] In one embodiment, the anti-inflammatory marker is IL-10.

[0229] A variety of assays are available to one of skill in the art that can determine whether TNF-alpha, IL-17 and / or IL-6 levels have been decreased or IL-10 levels have been increased. In one example, the concentrated levels of TNF-alpha, IL-17, IL-10 and / or IL-6 can be determined using spectrophotometric techniques such as the Immulite chemiluminescent immunoassay. In another example, the concentrated levels of TNF-alpha, IL-17, IL-10 and / or IL-6 can be measured using the Luminex platform using commercially available kits (Millipore).

[0230] In one example, administration of a cell composition comprising stem cells expressing high levels of Ang1 inhibits the release of TNF-alpha and / or IL-6 by macrophages. Various assays are available to those skilled in the art that can determine whether the release of TNF-alpha and / or IL-6 from macrophages is reduced. For example, macrophages are cultured in vitro and exposed to either a composition comprising stem cells expressing high levels of Ang1 or a suitable control. After a period of time, TNF-alpha and / or IL-6 release can then be assessed using the exemplary methods described above. The TNF-alpha and / or IL-6 levels in cells exposed to a cell composition comprising stem cells expressing high levels of Ang1 can then be compared to the TNF-alpha and / or IL-6 levels in control cells to determine whether the TNF-alpha and / or IL-6 levels are reduced.

[0231] Two distinct polarization states of macrophages have been identified: classically activated (M1) macrophage phenotype (i.e., "M1 macrophages") and alternatively activated (M2) macrophage phenotype (i.e., "M2 macrophages") (Gordon and Taylor., Nat. Rev. Immunol. 5:953-964, 2005; Mantovani et al., Trends Immunol. 23:549-555, 2002). M1 macrophages have a "pro-inflammatory" cytokine profile (e.g., TNF-alpha, IL-6, IL-1-beta, IL-12, IL-23). ​​In contrast, M2 macrophages have an "anti-inflammatory" cytokine profile (e.g., IL-10). In one embodiment, it is contemplated that administration of a cell composition comprising stem cells expressing high levels of Ang1 inhibits cytokine release by M1 macrophages. In another embodiment, administration of a cell composition comprising stem cells expressing high levels of Ang1 is envisioned to inhibit the release of TNF-alpha and / or IL-6 by M1 macrophages. A variety of assays are available to one skilled in the art that can determine whether the release of TNF-alpha, IL-6 and / or other cytokines by M1 macrophages is reduced. For example, M1 macrophages are used in the in vitro assays described above. In this embodiment, CD14+ mononuclear cells can be purified from a whole blood sample by immunoselection.

[0232] Increased production and / or function of anti-inflammatory cells In one embodiment, the present disclosure relates to a method of increasing the production and / or function of anti-inflammatory cells in a subject by administering stem cells that express high levels of Ang1.

[0233] The term "anti-inflammatory cells" is used in the context of this disclosure to refer to cells that induce or mediate an anti-inflammatory response in a subject. An "anti-inflammatory cell" may act directly on a cell population or target to induce an anti-inflammatory response. Alternatively, an anti-inflammatory cell encompassed by this disclosure may express or secrete factors, such as cytokines, that act on a particular cell population or target to induce an anti-inflammatory response.

[0234] Examples of anti-inflammatory cells include Th2 cells, Treg cells, and M2-type macrophages.

[0235] In one embodiment, the present disclosure relates to a method of increasing the number of Th2 cells, Tregs and / or M2-type macrophages in a subject by administering stem cells expressing high levels of Ang1.

[0236] In one embodiment, the present disclosure relates to a method of increasing the number of M2-type macrophages in a subject by administering stem cells that express high levels of Ang1.

[0237] In one example, the methods of the disclosure increase the number of M2-type macrophages in a subject to at least about 6%, at least about 7%, at least about 8%, or at least about 9% of the total mononuclear cell population.

[0238] In one example, the methods of the disclosure increase the number of M2-type macrophages in a subject to at least about 10% of the total mononuclear cell population.

[0239] In one example, the methods of the disclosure increase the number of M2-type macrophages in a subject by at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, or at least about 95% of the total mononuclear cell population.

[0240] One of skill in the art would be readily able to determine the percentage of M2 macrophages relative to the total mononuclear cell population of a subject using a variety of methods known in the art. For example, M2 macrophages can be identified based on the expression of CD14 and CD16 as well as other markers such as CD163 and CD206.

[0241] In this example, a whole blood sample can be obtained from a subject and cells can be immunoselected by FACS based on CD14 expression. CD14+ cells can then be assessed for expression of CD16, CD163 and CD206. The percentage of CD14+CD16+CD163+CD206+ can be calculated based on the total CD14+ cell population in the sample.

[0242] In one embodiment, increasing the production and / or function of anti-inflammatory cells in a subject is achieved by: A reduction in IL-6 levels in a subject; A reduction in TNF-alpha levels in a subject; and / or · Causing an increase in IL-10 levels in a subject.

[0243] In another embodiment, the method of the present disclosure is a method of promoting polarization of macrophages from an M1 to an M2 phenotype.

[0244] For example, the disclosure provides a method of promoting polarization of M1-type macrophages to M2-type macrophages in a subject in need thereof, the method comprising administering to the subject a composition comprising unaltered genetically stem cells, the unaltered genetically stem cells being at least 0.1 μg / 10 6 A large amount of cells express angiopoietin-1 (Ang1).

[0245] In this embodiment, CD14+CD16+ cells, CD14++CD16+ cells, CD14+CD16+CD163+ cells, CD14++CD16+CD163+ cells, CD14+CD16+CD206+ cells, CD14++CD16+CD206+ cells, CD14+CD16+CD163+CD206+ cells, CD14++CD16+CD163+CD206+ cells, CD14+CD163+ cells, An increase in the production or number of CD14++CD163+ cells, CD14+CD206+ cells, CD14++CD206+ cells, CD14+CD163+206+ cells, CD14++CD163+CD206+ cells, a decrease in IL-6 levels, a decrease in TNF-alpha levels, and / or an increase in IL-10 levels may indicate enhanced polarization of M1-type macrophages to M2-type macrophages.

[0246] Conversely, in another embodiment, the present disclosure provides a method for inhibiting polarization of M2 macrophages into M1 macrophages.

[0247] In another embodiment, the disclosure provides a method of inhibiting the production and / or function of M1-type macrophages in a subject in need thereof, the method comprising administering to the subject a composition comprising ungenetically modified stem cells, wherein the ungenetically modified stem cells express high levels of angiopoietin-1 (Ang1).

[0248] Treatment method The present disclosure relates to methods of treating inflammatory diseases.

[0249] As used herein, the term "inflammatory disease" should be considered to encompass diseases including, but not limited to, pruritus, skin inflammation, psoriasis, multiple sclerosis, rheumatoid arthritis, osteoarthritis, systemic lupus erythematosus, Hashimoto's thyroiditis, myasthenia gravis, diabetes mellitus type I or type II, diabetic nephropathy, asthma, inflammatory lung injury, inflammatory liver injury, inflammatory glomerular injury, atopic dermatitis, allergic contact dermatitis, irritant contact dermatitis, seborrheic dermatitis, Sjogren's syndrome, keratoconjunctivitis, uveitis, inflammatory bowel disease, Crohn's disease, ulcerative colitis, inflammatory diseases of the joints, skin or muscles, acute or chronic idiopathic inflammatory arthritis, myositis, demyelinating diseases, chronic obstructive pulmonary disease, interstitial lung disease, interstitial nephritis and chronic active hepatitis.

[0250] In one embodiment, the inflammatory disease treated by the method of the present disclosure is diabetes.In another embodiment, the inflammatory disease is a condition or symptom associated with diabetes.In this embodiment, the symptoms that can be treated include abnormal wound healing, symptoms associated with heart attack such as chest pain, symptoms associated with stroke, peripheral vascular disease, amputation, kidney disease, kidney failure, blindness, neuropathy, inflammation, impotence or non-alcoholic steatohepatitis (NASH).

[0251] For example, an inflammatory disease treated by the methods of the present disclosure is rheumatoid arthritis.

[0252] For example, an inflammatory disease treated by the methods of the present disclosure is diabetic retinopathy.

[0253] In one embodiment, the present disclosure relates to a method of treating diabetes.

[0254] In one embodiment, the present disclosure relates to a method of treating type II diabetes.

[0255] In one embodiment, the present disclosure relates to a method of treating diabetic nephropathy.

[0256] In one embodiment, the present disclosure relates to a method of treating rheumatoid arthritis.

[0257] As used herein, the term "treat" or "treatment" or "treating" should be understood to mean administering a therapeutically effective amount of cells. In the context of the present disclosure, the term "therapeutically effective amount of cells" refers to an amount of cells that is effective to prevent, ameliorate or treat an inflammatory disease or disorder. Such an effective amount will generally result in an improvement in the signs, symptoms and / or other indicators of an inflammatory disease or disorder. For example, in the case of diabetes, an effective amount of cells can result in a reduction in HbA1c levels, a reduction in cytokine levels such as IL-6 and / or TNF-α, a reduction in fasting insulin and / or an increase in adiponectin levels.

[0258] For example, in the case of rheumatoid arthritis, an effective amount of cells may result in the achievement of ACR20, ACR50 and / or ACR70, a decrease in cytokine levels such as IL-6 and / or a decrease in disease activity scores.

[0259] For example, in the case of diabetic nephropathy, an effective amount of cells can result in inhibition of decline in eGFR or mGFR, improvement in eGFR or mGFR and / or reduction in cytokine levels such as IL-6.

[0260] A variety of routine clinical assays are available to those skilled in the art that can be used to identify reduced HbA1c levels, reduced fasting insulin and / or increased adiponectin levels in the context of diabetes or its related conditions or symptoms. For example, a blood sample is typically obtained from a subject and then subjected to immunoassays to detect HbA1c, insulin and adiponectin levels.

[0261] Cell culture method In one embodiment, a method for generating stem cells expressing high levels of Ang1 comprises culturing a population of stem cells in a cell culture medium comprising a short-acting L-ascorbic acid derivative but not comprising an equivalent amount of a long-acting L-ascorbic acid derivative and / or supplemented with less than 10% (v / v) fetal bovine serum.

[0262] The term "media" or "medium" as used in relation to cell culture includes components of the environment surrounding the cells. It is envisioned that the medium contributes to and / or provides conditions sufficient to induce expression of Ang1 expression. The medium may be solid, liquid, gaseous, or a mixture of phases and substances. Media can include liquid growth media and liquid media that do not support cell growth. Media also include gelatinous media such as agar, agarose, gelatin, and collagen matrices. Exemplary gaseous media include the gas phase to which cells growing on a Petri dish or other solid or semi-solid support are exposed. The term "media" also refers to a substance intended for use in cell culture, even if it is not yet in contact with the cells.

[0263] The culture medium used in the method for producing stem cells expressing high levels of Ang1 can be prepared using a medium used for culturing stem cells as the basal medium. The basal medium includes, for example, Eagle's minimum essential (MEM) medium, α-modified MEM medium, and mixed culture medium thereof, and is not particularly limited as long as it can be used for culturing stem cells.

[0264] In addition, the culture medium can contain components such as fatty acids or lipids, vitamins, growth factors, cytokines, antioxidants, buffering agents, inorganic salts, and the like.

[0265] The cell culture medium can contain all essential and optionally non-essential amino acids, which are generally classified as essential (threonine, methionine, valine, leucine, isoleucine, phenylalanine, tryptophan, lysine, histidine) and non-essential (glycine, alanine, serine, cysteine, glutamine, asparagine, aspartic acid, tyrosine, arginine, proline).

[0266] Ascorbic acid is an essential supplement for the growth and differentiation of various cells in culture. It is now understood that certain ascorbic acid derivatives are "short-acting" because they are not stable in solution, especially under normal cell culture conditions at neutral pH and 37°C. These short-acting derivatives are rapidly oxidized to oxalic acid or threonic acid. In culture medium at 37°C (pH 7), the levels of the short-acting ascorbic acid derivatives are reduced by approximately 80-90% after 24 hours due to oxidation. Thus, the short-acting ascorbic acid derivatives are replaced by the more stable "long-acting" ascorbic acid derivatives in conventional cell cultures of various cell types.

[0267] In the context of the present disclosure, the term "short-acting" includes ascorbic acid derivatives that are approximately 80-90% oxidized after 24 hours of cell culture under culture conditions of neutral pH and 37° C. In one embodiment, the short-acting L-ascorbic acid derivative is an L-ascorbate salt. For example, in the context of the present disclosure, sodium L-ascorbate salt is a "short-acting" ascorbic acid derivative.

[0268] In contrast, the term "long-acting" includes ascorbic acid derivatives that are not oxidized by about 80-90% after 24 hours in cell culture under culture conditions of neutral pH and 37°C. In one embodiment, in the context of the present disclosure, L-ascorbic acid 2-phosphate is a "long-acting" ascorbic acid derivative. Other examples of long-acting ascorbic acid derivatives include tetrahexyldecyl ascorbate, magnesium ascorbyl phosphate, and 2-O-α-D-glucopyranosyl-L-ascorbic acid.

[0269] In one embodiment, the culture medium used in the method for generating stem cells expressing high levels of Ang1 is supplemented with a short-acting ascorbic acid derivative. For example, the cell culture medium may comprise at least about 0.005 g / L of a short-acting ascorbic acid derivative. In another embodiment, the cell culture medium may comprise at least about 0.01 g / L of a short-acting ascorbic acid derivative. For example, the cell culture medium may comprise at least about 0.02 g / L of a short-acting ascorbic acid derivative. In another embodiment, the cell culture medium may comprise at least about 0.03 g / L of a short-acting ascorbic acid derivative. For example, the cell culture medium may comprise at least about 0.04 g / L of a short-acting ascorbic acid derivative. In another embodiment, the cell culture medium may comprise at least about 0.05 g / L of a short-acting ascorbic acid derivative. In another embodiment, the cell culture medium may comprise at least about 0.06 g / L of a short-acting ascorbic acid derivative. In one example of this embodiment, the cell culture medium is supplemented with the sodium salt of L-ascorbic acid.

[0270] In another embodiment, the cell culture medium includes a short-acting ascorbic acid derivative but does not include a significant amount of a long-acting ascorbic acid derivative. For example, the cell culture medium may include a short-acting ascorbic acid derivative but not more than 0.04 g / L of the long-acting ascorbic acid derivative. In another embodiment, the cell culture medium may include a short-acting ascorbic acid derivative but not more than 0.03 g / L of the long-acting ascorbic acid derivative. In another embodiment, the cell culture medium may include a short-acting ascorbic acid derivative but not more than 0.02 g / L of the long-acting ascorbic acid derivative. In another embodiment, the cell culture medium may include a short-acting ascorbic acid derivative but not more than 0.01 g / L of the long-acting ascorbic acid derivative. In another embodiment, the cell culture medium may include a short-acting ascorbic acid derivative but not more than 0.005 g / L of the long-acting ascorbic acid derivative. In another embodiment, the cell culture medium may include a short-acting ascorbic acid derivative, but may not include a long-acting ascorbic acid derivative.

[0271] In another embodiment, the cell culture medium contains L-ascorbic acid sodium salt but does not contain an equivalent amount of L-ascorbic acid-2-phosphate.

[0272] The cell culture medium used in the method for generating stem cells expressing high levels of Ang1 can be a serum-containing medium or a serum-free medium.

[0273] The culture medium may or may not contain a serum replacement. The serum replacement may be, for example, albumin (e.g., lipid-rich albumin), transferrin, fatty acids, insulin, collagen precursors, trace elements, 2-mercaptoethanol or 3'-thiolglycerol, or a serum equivalent that contains them appropriately. Such serum replacements may be prepared, for example, by the methods described in International Patent Application No. 93 / 30679, or may be commercially available.

[0274] In one embodiment, the cell culture medium used in the method of generating stem cells expressing high levels of Ang1 is supplemented with at least about 9% (v / v), at least about 8% (v / v), at least about 7% (v / v), at least about 6% (v / v), at least about 5% (v / v), at least about 4% (v / v), at least about 3% (v / v), at least about 2% (v / v), at least about 1% (v / v) FCS. It is also contemplated that the terms fetal calf serum (FCS) and fetal bovine serum (FBS) may be used interchangeably in the context of the present invention.

[0275] In one embodiment, the cell culture medium is supplemented with non-fetal serum. It is contemplated that the medium may be supplemented with at least about 1% (v / v), at least about 2% (v / v), at least about 3% (v / v), at least about 4% (v / v), at least about 5% (v / v), at least about 6% (v / v), at least about 7% (v / v), at least about 8% (v / v), at least about 9%, at least about 10%, at least about 11%, at least about 12%, at least about 13%, at least about 14%, at least about 15%, at least about 16%, at least about 17%, at least about 18%, at least about 19%, at least about 20%, at least about 21%, at least about 22%, at least about 23%, at least about 24%, at least about 25% (v / v) of non-fetal serum.

[0276] For example, the culture medium can be supplemented with non-fetal mammalian serum.

[0277] For example, the culture medium can be supplemented with human non-fetal serum.

[0278] For example, the culture medium can be supplemented with neonatal serum. It is contemplated that the medium may be supplemented with at least about 1% (v / v), at least about 2% (v / v), at least about 3% (v / v), at least about 4% (v / v), at least about 5% (v / v), at least about 6% (v / v), at least about 7% (v / v), at least about 8% (v / v), at least about 9%, at least about 10%, at least about 11%, at least about 12%, at least about 13%, at least about 14%, at least about 15%, at least about 16%, at least about 17%, at least about 18%, at least about 19%, at least about 20%, at least about 21%, at least about 22%, at least about 23%, at least about 24%, at least about 25% (v / v) of neonatal serum.

[0279] In one embodiment, the cell culture medium is supplemented with neonatal mammalian serum.

[0280] For example, the culture medium can be supplemented with newborn bovine serum (NBCS). It is contemplated that the medium may be supplemented with at least about 1% (v / v), at least about 2% (v / v), at least about 3% (v / v), at least about 4% (v / v), at least about 5% (v / v), at least about 6% (v / v), at least about 7% (v / v), at least about 8% (v / v), at least about 9%, at least about 10%, at least about 11%, at least about 12%, at least about 13%, at least about 14%, at least about 15%, at least about 16%, at least about 17%, at least about 18%, at least about 19%, at least about 20%, at least about 21%, at least about 22%, at least about 23%, at least about 24%, or at least about 25% (v / v) of NBCS.

[0281] In one embodiment, the cell culture medium is supplemented with human newborn serum.

[0282] For example, the cell culture medium can be supplemented with at least about 1% (v / v), at least about 2% (v / v), at least about 3% (v / v), at least about 4% (v / v), at least about 5% (v / v), at least about 6% (v / v), at least about 7% (v / v), at least about 8% (v / v), at least about 9% (v / v) of human newborn serum. For example, human newborn serum is obtained from "umbilical cord blood".

[0283] In one embodiment, the culture medium is supplemented with adult serum. It is contemplated that the medium may be supplemented with at least about 1% (v / v), at least about 2% (v / v), at least about 3% (v / v), at least about 4% (v / v), at least about 5% (v / v), at least about 6% (v / v), at least about 7% (v / v), at least about 8% (v / v), at least about 9%, at least about 10%, at least about 11%, at least about 12%, at least about 13%, at least about 14%, at least about 15%, at least about 16%, at least about 17%, at least about 18%, at least about 19%, at least about 20%, at least about 21%, at least about 22%, at least about 23%, at least about 24%, at least about 25% (v / v) of adult serum.

[0284] In one embodiment, the cell culture medium is supplemented with adult mammalian serum.

[0285] For example, the cell culture medium can be supplemented with at least about 1% (v / v), at least about 2% (v / v), at least about 3% (v / v), at least about 4% (v / v), at least about 5% (v / v), at least about 6% (v / v), at least about 7% (v / v), at least about 8% (v / v), at least about 9%, at least about 10%, at least about 11%, at least about 12%, at least about 13%, at least about 14%, at least about 15%, at least about 16%, at least about 17%, at least about 18%, at least about 19%, at least about 20%, at least about 21%, at least about 22%, at least about 23%, at least about 24%, at least about 25% (v / v) of adult mammalian serum.

[0286] For example, the cell culture medium can be supplemented with at least about 1% (v / v), at least about 2% (v / v), at least about 3% (v / v), at least about 4% (v / v), at least about 5% (v / v), at least about 6% (v / v), at least about 7% (v / v), at least about 8% (v / v), at least about 9%, at least about 10%, at least about 11%, at least about 12%, at least about 13%, at least about 14%, at least about 15%, at least about 16%, at least about 17%, at least about 18%, at least about 19%, at least about 20%, at least about 21%, at least about 22%, at least about 23%, at least about 24%, at least about 25% (v / v) adult bovine serum.

[0287] In one embodiment, the cell culture medium is supplemented with adult human serum.

[0288] For example, the cell culture medium can be supplemented with at least about 1% (v / v), at least about 2% (v / v), at least about 3% (v / v), at least about 4% (v / v), at least about 5% (v / v), at least about 6% (v / v), at least about 7% (v / v), at least about 8% (v / v), at least about 9% (v / v) human adult serum.

[0289] For example, the cell culture medium can be supplemented with at least about 1% (v / v), at least about 2% (v / v), at least about 3% (v / v), at least about 4% (v / v), at least about 5% (v / v), at least about 6% (v / v), at least about 7% (v / v), at least about 8% (v / v), at least about 9% (v / v) of human AB serum.

[0290] In one embodiment, the cell culture medium is supplemented with at least about 3% human AB serum.

[0291] In one embodiment, the culture medium is supplemented with a mixture of FCS and NBCS.

[0292] For example, the culture medium can be supplemented with a mixture of FCS and NBCS such that the ratio of FCS:NBCS is at least about 0.4:1, at least about 0.5:1, at least about 0.6:1, at least about 0.7:1, at least about 0.8:1, at least about 0.9:1, at least about 1:1, at least about 1.5:1, at least about 2:1.

[0293] For example, it is contemplated that the mixture of FCS and NBCS may comprise at least about 1% (v / v), at least about 2% (v / v), at least about 3% (v / v), at least about 4% (v / v), at least about 5% (v / v), at least about 6% (v / v), at least about 7% (v / v), at least about 8% (v / v), at least about 9%, at least about 10%, at least about 11%, at least about 12%, at least about 13%, at least about 14%, at least about 15%, at least about 16%, at least about 17%, at least about 18%, at least about 19%, at least about 20%, at least about 21%, at least about 22%, at least about 23%, at least about 24%, at least about 25% (v / v) of the cell culture medium. However, in this embodiment, the cell culture medium is supplemented with at least about 1% (v / v), at least about 2% (v / v), at least about 3% (v / v), at least about 4% (v / v), at least about 5% (v / v), at least about 6% (v / v), at least about 7% (v / v), at least about 8% (v / v), at least about 9% (v / v), but less than 10% (v / v) FCS.

[0294] In one embodiment, the cell culture medium is FCS serum-free.

[0295] In one embodiment, the cell culture medium is fetal serum free.

[0296] In one embodiment, the cell culture medium is supplemented with non-fetal serum.

[0297] In one embodiment, the cell culture medium is fetal serum free and supplemented with non-fetal serum.

[0298] In another embodiment, the cell culture medium is supplemented with one or more stimulatory factors selected from the group consisting of 1α,25-dihydroxyvitamin D3 (1,25D), platelet-derived growth factor (PDGF), tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), and stromal-derived factor alpha (SDF-1α). In another embodiment, the cells can be cultured in the presence of at least one cytokine in an amount sufficient to support proliferation of the cells.

[0299] In another embodiment, the cells are cultured in the presence of a platelet cell lysate in an amount sufficient to support the growth of the cells. For example, the cells can be cultured with a human platelet cell lysate in an amount sufficient to support the growth of the cells.

[0300] In one embodiment, the cells are cultured with human AB serum and human platelet lysate in an amount sufficient to support cell growth.

[0301] Moreover, those skilled in the art can generate stem cells expressing high levels of Ang1 using the methods exemplified below.

[0302] Analyzing the therapeutic / prophylactic potential of cells Methods for determining the ability of stem cells expressing high levels of Ang1 to treat, prevent, or delay the onset or progression of a disease will be apparent to one of skill in the art. For example, stem cells can be assessed for their ability to increase the levels of Ang1.

[0303] In one embodiment, at least 0.1 μg / 10 6 Unmodified stem cells expressing Ang1 in cellular quantities are tested for their ability to increase Ang1 expression in vitro and / or in vivo. In these examples, cells or tissues are evaluated for the evolution of Ang1 expression following administration of stem cells expressing high levels of Ang1.

[0304] It will be apparent to one of skill in the art from the above that the present disclosure also provides the following methods for identifying or isolating cells for treating, preventing or delaying disease. (i) Stem cells expressing high levels of Ang1 are administered to a test subject suffering from the relevant disease and the subject is assessed for symptoms of the disease. (ii) comparing symptoms of the disorder in the subject of (i) with symptoms or activity of the disease in a control subject suffering from the disease but not administered the stem cells, where an improvement in the symptoms in the test subject compared to the control subject indicates that the stem cells treat the disease. The cells may be any cell described in this disclosure, according to any embodiment. EXAMPLES

[0305] Example 1: STRO-3 + Immunoselection of MPCs by cell selection Bone marrow (BM) is collected from healthy normal adult volunteers (20-35 years old). Briefly, 40 ml of BM is aspirated from the posterior iliac crest into a lithium-heparin anticoagulant-containing tube.

[0306] Bone marrow mononuclear cells (BMMNC) are prepared by density gradient separation using Lymphoprep™ (Nycomed Pharma, Oslo, Norway) as previously described (Zannettino et al., Blood, 92:2613-2628, 1998). After centrifugation at 400×g and 4° C. for 30 min, the pale yellow layer is removed with a transfer pipette and washed three times in “HHF” consisting of Hanks’ Balanced Salt Solution (HBSS; Life Technologies, Gaithersburg, MD) containing 5% fetal calf serum (FCS, CSL Limited, Victoria, Australia).

[0307] Next, STRO-3 + (or TNAP +) cells were isolated by magnetic activated cell sorting as previously described (Gronthos et al., Journal of Cell Science 116:1827-1835, 2003; Gronthos and Simmons, Blood, 85, 929-940, 1995). Briefly, approximately 1-3 × 10 8 BMMNCs are incubated on ice for 20 min in blocking buffer consisting of 10% (v / v) normal rabbit serum in HHF. The cells are incubated on ice for 1 h with 200 μl of a 10 μg / ml STRO-3 mAb solution in blocking buffer. The cells are then washed twice in HHF by centrifugation at 400×g. Goat anti-mouse γ-biotin (Southern Biotechnology Associates, Birmingham, UK) diluted 1 / 50 in HHF buffer is added and the cells are incubated on ice for 1 h. The cells are then incubated in MACS buffer (Ca 2+ 0.5% CO2 supplemented with 1% BSA, 5 mM EDTA and 0.01% sodium azide) as above. 2+ and Mn 2+ The cells are washed twice in PBS (without HCl) and resuspended in a final volume of 0.9 ml MACS buffer.

[0308] 100 μl of streptavidin microbeads (Miltenyi Biotec; Bergisch Gladbach, Germany) are added to the cell suspension and incubated on ice for 15 min. The cell suspension is washed twice with 0.5 ml of MACS buffer, resuspended, and then loaded onto a mini MACS column (MS Columns, Miltenyi Biotec) and washed three times with 0.5 ml of MACS buffer to recover cells that did not bind to STRO-3mAb (deposited at the American Type Culture Collection (ATCC) on December 19, 2005 under accession number PTA-7282; see International Patent Application No. 2006 / 108229). After adding a further 1 ml of MACS buffer, the column is removed from the magnet and TNAP +Cells are isolated by positive pressure. An aliquot of cells from each fraction can be stained with streptavidin-FITC and purity assessed by flow cytometry.

[0309] MPCs isolated in this way express STRO-1 bright It is MPC.

[0310] Example 2: Starting Medium - Process A Alpha modifications of Eagle's minimum essential medium (MEM) with Earle's balanced salts, commonly referred to as Eagle's αMEM, contain non-essential amino acids, sodium pyruvate, and supplemented vitamins. These modifications were first described for use in growing mouse-hamster hybrid cells (Stanners et al., Nat New Biol., 230, 52-54, 1971).

[0311] Eagle's αMEM medium, suitable for culturing primary stem cells, can be obtained from a variety of sources, such as Life Technologies and Sigma.

[0312] Detailed methods for establishing primary stem cell cultures containing the necessary growth factors used in the exemplary process are described in Gronthos and Simmons, Blood, 85, 929-940, 1995. In process A, Eagle's αMEM medium supplemented with 10% fetal bovine serum, L-ascorbic acid-2-phosphate (100 μM), dexamethasone (10−7 M), and / or inorganic phosphate (3 mM) was used to culture the stem cells.

[0313] Example 3: Modified Culture Medium - Process B In Process B, the Eagle's αMEM medium used in Process A was modified (modified αMEM) with: ·Replace the long-acting ascorbic acid derivative L-ascorbic acid-2-phosphate with the short-acting ascorbic acid derivative sodium L-ascorbate (50 mg / L). Reduce FCS from 10% (v / v) to 5% (v / v). ·-Supplement with non-fetal serum (5% v / v).

[0314] [Table 1]

[0315] Example 4: Cell Culture Mesenchymal precursor cells (MPCs) were obtained from a single donor and stored using the following cryopreservation method.

[0316] In general, the cell culture included the following steps.

[0317] Thaw cryopreserved MPCs and inoculate them at 10,000 cells / cm. 2 1. Seed in 20% O / 200 mL of PBS until 90% confluence in either starting medium (process A, n = 3) or modified culture medium (process B, n = 3). 2 , and grown at 37°C.

[0318] To generate conditioned medium, growth medium was added to 200 μl medium / cm 2 The medium was replaced with EBM-2 basal medium (Lonza) supplemented with FCS at a volume of 100 μg / ml. The cells were cultured for an additional 3 days, after which the medium was harvested and centrifuged to remove any cells, and the resulting supernatant was collected and stored at -80°C.

[0319] Growth factor concentrations were measured using the Luminex platform using commercially available kits (Millipore).

[0320] Following cell culture, MPC proliferation dynamics were assessed (see Figures 1-3). No significant changes in cell proliferation, MPC doubling time or population doubling time were observed following cell culture processes A and B.

[0321] MPCs are also characterized in terms of expression levels of the cell markers STRO-1, CC9 and STRO-4 as well as the pro-angiogenic growth factors Ang1 and VEGF.

[0322] Levels of STRO-1, CC9 and STRO-4 were comparable in MPCs after cell culture processes A and B. However, the cultivation process B is Increases Ang1 levels, Reduces VEGF levels, Provides a ratio of Ang1:VEGF that is consistent with Ang1:VEGF ratios previously shown to be particularly effective in enhancing angiogenesis. Ang1 and VEGF levels (μg / 10 6 The measured values ​​for the number of cells are shown in Table 2.

[0323] [Table 2]

[0324] Example 5: Modified Culture Conditions - Processes C and D To control for the exchange of the long-acting ascorbic acid derivative, L-ascorbic acid-2-phosphate, with the short-acting ascorbic acid derivative sodium L-ascorbate, MPCs from three different donors were serially propagated in α-MEM + 10% FCS + 50 mg / L sodium L-ascorbate (Process C) or α-MEM + 3% human AB serum + 50 mg / L sodium L-ascorbate (Process D) with growth factors such as PDGF and EGF.

[0325] Ang1 and VEGF levels were assessed after cell culture in Processes C and D. Ang1 and VEGF levels (ug / 10 6 Cells) are shown in Table 3.

[0326] Compared to process C, cultivation process D has the following advantages: Increases Ang1 levels, Reduces VEGF levels, ·Increases the VEGF:Ang1 ratio. Compared to Process A, Processes C and D lead to a progressive increase in the expression levels of Ang1, suggesting that the short-acting ascorbic acid derivative and the presence of non-fetal serum each independently lead to increased expression of Ang1 and exert a synergistic effect to further increase Ang1 expression.

[0327] [Table 3]

[0328] Example 6: Polarization of proinflammatory M1 mononuclear cells to the M2 phenotype CD14+ mononuclear cells were immunoselected from whole blood. The mononuclear cell population was characterized based on CD16 expression. 5.2% of the cells had the phenotypic characteristics of M2-type macrophages (CD14 + CD16 + ) was shown (Figure 4).

[0329] CD14+ mononuclear cells were co-cultured for 7 days with MPCs expressing high levels of Ang1. MPCs were obtained from two different donors (#023 and #009).

[0330] After 3 days of co-culture, cells were assessed for: CD14, CD16 expression; and · TNF-α secretion in response to lipopolysaccharide (LPS). LPS was added at 1 ng / ml for 24 h (+transduction inhibitor for the last 5 h).

[0331] Co-culture resulted in the following: Phenotypic characteristics of M2-type macrophages (CD14 + CD16 + CD163 + CD206 + ) generation of macrophages exhibiting inflammatory responses (Figure 4) · Inhibition of proinflammatory (LPS) secretion of TNFα by macrophages (Figure 5)

[0332] After 7 days of co-culture, cells were assessed for secretion of IL-10 in response to LPS. LPS was added at 1 ng / ml for 24 h (+transduction inhibitor for the last 5 h). IL-10 expression was analyzed by intracellular flow cytometry. IL-10 production by macrophages was enhanced by co-culture with MPC in the presence of LPS (Figure 6).

[0333] These data indicate that MPCs expressing high levels of Ang1 promote the production of M2-type macrophages (Figure 7).

[0334] Stem cells expressing high levels of Ang1 were cultured with increasing amounts of IL-1β alone or in combination with TNF-α, and the effects of these cytokines on PGE2 secretion were assessed.

[0335] An additive effect of IL-1β and TNF-α on PGE2 expression was observed (Figure 8).

[0336] PGE2 promotes the polarization of M1 macrophages into M2 macrophages, as well as the differentiation of Th17 cells into Th2 and TReg cells (Figure 7). High IL-1 and TNF-α levels have been observed in subjects suffering from inflammatory diseases, such as type II diabetes.

[0337] Thus, the increased secretion of PGE2 from stem cells expressing high levels of Ang1 in response to TNFα and IL1β suggests that stem cells expressing high levels of Ang1 may increase the production of anti-inflammatory cells in subjects suffering from inflammatory diseases. In particular, stem cells expressing high levels of Ang1 Increase M2 macrophage production by promoting the polarization of M1 macrophages into M2 macrophages; and / or By promoting the differentiation of Th17 cells, it can increase the production of Th2 and Treg.

[0338] Example 7: Sheep model of collagen-induced arthritis Stem cells expressing high levels of Ang1 were administered to an ovine model of rheumatoid arthritis (Thorpe et al., Clinical & Exp. Rheumatology, 10:143-150 (1992)). This model is characterized by inflammatory manifestations of rheumatoid arthritis, both systemic and articular.

[0339] 150 million cryopreserved ovine MPCs were administered intravenously via the jugular vein.

[0340] IL-17 and IL-10 levels were assessed over a 2-week period. Changes in the levels of pro- and anti-inflammatory cytokines are shown in (Figure 9).

[0341] Stem cells expressing high levels of Ang1 were also administered at the time of establishment of late stage disease (day 42). Administration of stem cells led to a reduction in the levels of inflammatory cytokines in the joints (Figure 10).

[0342] Example 8: Stem Cell Administration in Diabetes Human subjects with type 2 diabetes inadequately controlled with metformin or metformin plus another oral medication were infused with a single intravenous injection of three doses of mesenchymal progenitor cells (MPCs) compared with a placebo control. Changes in baseline HbA1c, IL-6, TNF-alpha, fasting insulin, adiponectin, osteocalcin, and hsCRP were assessed over 12 weeks.

[0343] Subjects were divided into three cohorts (Figure 11). Cohort 1: 1 dose of MPC (0.3 million cells / kg) [n=15] or placebo [n=5] Cohort 2: 1 dose of MPC (1 million cells / kg) [n=15] or placebo [n=5] Cohort 3: 1 dose of MPC (2 million cells / kg) [n=15] or placebo [n=5]

[0344] A small decrease in HbA1c was observed in subjects treated with MPC compared to placebo-controlled subjects who had a small increase (Table 4). A larger decrease in HbA1c was observed in cohort 2 vs. placebo at week 8 (Table 4). A trend for a larger decrease in HbA1c was observed in subjects with baseline HbA1c values ​​≥ 8% (Figure 15). Eight of 45 (17.8%) subjects achieved target HbA1c (< 7.0%) at week 12 (Figure 16). A trend towards improved fasting insulin and adiponectin levels (Figure 12) was observed in MPC-treated subjects compared to placebo-controlled subjects. There was a decrease in TNF-alpha and IL-6 levels in MPC-treated subjects compared to placebo-controlled subjects, with the most significant decrease observed in cohort 3 (Figure 13). In cohort 3, the change from baseline in TNF-alpha (Figure 13) and IL-6 (Figure 14) was -0.26 pg / ml and -0.47 pg / ml, respectively (Figure 13).

[0345] [Table 4]

[0346] Example 9: Stem cell administration in rheumatoid arthritis Human subjects with rheumatoid arthritis classified as inadequate anti-TNFα responders or who had failed up to two alternative biologics were infused with a single intravenous injection of two doses of mesenchymal progenitor cells (MPCs) and compared with a placebo control.

[0347] 48 subjects were included in the study: +RF / anti-CCP; >4 swollen / tender joints; ESR / CRP>ULN.

[0348] Subjects were divided into two cohorts. Cohort 1: MPC dose (1 million cells / kg) [n=16] or placebo [n=8] Cohort 2: MPC dose (2 million cells / kg) [n=16] or placebo [n=8]

[0349] Assessment endpoints 3 months after injection included: - TNFα; IL-6 (Figure 17), IL-17; RANKL; MMP-1, 3, 9; TIMP-1, 2, 4 and osteocalcin levels. Levels were also assessed at 0, 1, 2, 4, 6, 8 and 10 weeks. ACR20 (Fig. 18) / 50 (Fig. 19) / 70 (Fig. 20); · ACR individual assets (Fig. 21; Fig. 22); Remission (Disease Activity Score (DAS28(CRP))<2.6) (Figure 23); Change from baseline in Disease Activity Score (DAS28); ESR / CRP; HAQ-DI (Figure 24); SF-36 (remission DAS28(CRP)<2.6); response DAS28(CRP)<3.2 (Figure 23); Hand / wrist x-rays at 6 and 12 months

[0350] There were no treatment-related serious adverse events (SAEs) associated with Cohort 1. Early and sustained efficacy was observed through 3 months.

[0351] Over weeks 1-12, IL-6 levels decreased from baseline in cohort 1 compared to placebo (Figure 17).

[0352] The data support the suggestion of a potential remission rate of approximately 20%, which may be higher than other biologics (Figure 23).

[0353] The primary endpoint at 12 weeks for Cohort 1 revealed a consistent trend towards improved response over placebo.

[0354] Follow-up interim analyses show persistence of responder effects over time.

[0355] Cohort 2 is undergoing testing with a larger single dose.

[0356] Example 10: Stem cell administration in diabetic nephropathy Human subjects with type 2 diabetes and moderate to severe chronic kidney disease on a stable regimen of ACEi or ARB therapy for diabetic nephropathy were infused with a single intravenous injection of two doses of mesenchymal progenitor cells (MPCs) and compared with a placebo control.

[0357] Subjects were divided into two cohorts. Cohort 1: 1 dose of MPC (150 million cells / kg) [n=10] or placebo [n=5] Cohort 2: MPC 1 dose (300 million cells / kg) [n=10] or placebo [n=5]

[0358] Endpoints assessed 3 and 6 months after injection: Measured GFR (mGFR[99Tc DTPA]) (Figure 25), estimated GFR (eGFR[MDRD]) (Figure 25), IL-6 (Figure 26); and serum creatinine levels; Correlation between IL-6 and serum creatinine levels (Figure 27)

[0359] A trend towards prevention or improvement of renal function by both measured and estimated GFR was observed in MPC-treated subjects versus placebo during the initial 24-week study period. The therapeutic effects were similar for both MPC doses. Baseline eGFR>30ml / min / 1.73m 2 The therapeutic effect of MPC was more pronounced in subjects with A more pronounced treatment effect with MPC in subjects with baseline IL-6 levels above the median; · A significant correlation between baseline IL-6 levels and MPC-associated improvement in serum creatinine (Figure 27); There was a dose-dependent change in serum IL-6 levels at week 12 in the MPC group vs. placebo (Figure 26).

[0360] It will be understood by those skilled in the art that numerous variations and / or modifications may be made to the present disclosure as illustrated in the specific embodiments without departing from the spirit or scope of the disclosure as broadly described, and the present embodiments are therefore to be considered in all respects as illustrative and not restrictive.

[0361] This application claims priority to AU2014902194, filed June 10, 2014, and AU2014902257, filed June 13, 2014, the disclosures of which are incorporated herein by reference.

[0362] All publications discussed and / or referenced herein are incorporated herein in their entirety.

[0363] Any discussion of documents, acts, materials, devices, articles or the like which has been included in the present specification is solely for the purpose of providing a context for the present invention and should not be understood as an admission that any or all of such matter existed prior to the priority date of each claim of this application and therefore forms part of the prior art base or is common general knowledge in the field relevant to the present invention.

Claims

1. 1. A composition for treating an inflammatory disease in a subject, the composition comprising cultured expanded genetically unmodified stem cells, the genetically unmodified stem cells expressing angiopoietin-1 (Ang1) and vascular endothelial growth factor (VEGF), the genetically unmodified stem cells being administered at a concentration of 0.01 μg / 10 ６ 0.05μg / 10 from cells ６ The composition expresses VEGF in amounts between 10 and 20% of cells.

2. The genetically unmodified stem cells were ６ The composition of claim 1 , which expresses vascular endothelial growth factor (VEGF) in subcellular amounts.

3. The genetically unmodified stem cells were ６ The composition of claim 1 , which expresses vascular endothelial growth factor (VEGF) in subcellular amounts.

4. The composition of claim 1 , wherein the genetically unmodified stem cells are mesenchymal progenitor cells.

5. The composition of claim 1 , wherein the stem cells are mesenchymal stem cells.

6. 6. The composition of any one of claims 1 to 5, wherein the inflammatory disease is diabetes or a diabetes-related condition or symptom selected from the group consisting of abnormal wound healing, heart attack symptoms, stroke symptoms, peripheral vascular disease symptoms, amputation, kidney disease symptoms, renal failure, blindness, neuropathy, nephropathy, retinopathy, inflammation, impotence or non-alcoholic steatohepatitis (NASH).

7. The composition of any one of claims 1 to 5, wherein the inflammatory disease is type II diabetes.

8. The composition of any one of claims 1 to 5, wherein the inflammatory disease is inflammatory lung injury.

9. The composition of any one of claims 1 to 5, wherein the inflammatory disease is an interstitial lung disease.

10. The composition of any one of claims 1 to 5, wherein the inflammatory disease is rheumatoid arthritis.

11. About 0.1×10 per kg of the subject ６ pieces ~ approx. 3×10 ６ The composition of claim 1 , comprising stem cells.

12. Approximately 2×10 per kg of the subject ６ The composition of claim 1 , comprising stem cells.

13. 13. The composition of any one of claims 1 to 12, comprising multiple doses.

14. A composition according to any one of claims 1 to 13, which increases the production and / or function of anti-inflammatory cells in a subject.

15. The composition of claim 14, which increases the production and / or function of Th2 cells, TReg cells, or M2-type macrophages.

16. The genetically unmodified stem cells were ６ 1.0 μg / 10 from cells ６ 16. The composition of claim 1, wherein the cell expresses Ang1 in amounts between 1 and 15.

17. 1. Use of a composition comprising culture-expanded genetically unmodified mesenchymal progenitor-lineage cells in the manufacture of a medicament for treating an inflammatory disease to increase production and / or function of anti-inflammatory cells in a subject, wherein the genetically unmodified mesenchymal progenitor-lineage cells express angiopoietin-1 (Ang1) and vascular endothelial growth factor (VEGF), and the genetically unmodified mesenchymal progenitor-lineage cells are cultured at a concentration of 0.01 μg / 10 ６ 0.05μg / 10 from cells ６ Use of cells that express VEGF in amounts between 10 and 20%.

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