An agent for promoting NO production, improving explosive power in lower limb muscles, and reducing muscle damage caused by intensive exercise.

Abstract

To provide a novel NO production enhancer.SOLUTION: The above problem is solved by the following technological characteristics of the invention: 1) a NO production enhancer including as active principle 1,2-di-O-galloyl-4,6-O-(S)-hexahydroxydiphenoyl-β-D-glucose; 2) a NO production enhancer including as active principle a composition containing 1,2-di-O-galloyl-4,6-O-(S)-hexahydroxydiphenoyl-β-D-glucose; and 3) a NO production enhancer including as active principle 1,2-di-O-galloyl-4,6-O-(S)-hexahydroxydiphenoyl-β-D-glucose derived from purple tea (scientific name: Camellia sinensis; variety name: TRFK306).SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to an agent for promoting NO production. The present invention is widely used in foods, medicines, cosmetics, etc. [Background technology]

[0002] NO (nitric oxide) is produced when L-arginine is oxidized to L-citrulline, and this reaction is catalyzed by NO synthase (NOS). NO production is observed in various tissues and cell types in the body, but vascular endothelial cells are a representative cell type that constantly produces and releases NO. It has been reported that NO produced in vascular endothelial cells (endothelial NO) is endothelium-derived vasorelaxing factor (EDRF) (see Non-Patent Documents 1 and 2). Therefore, compounds that promote spontaneous and sufficient NO production in vascular endothelial cells and compounds that have eNOS activation activity are thought to improve vascular endothelial function and are excellent therapeutic agents for diseases caused by reduced vascular endothelial function, such as blood flow disorders, arteriosclerosis, hyperlipidemia, ischemic heart disease, and circulatory failure in various organs. Therefore, compounds with NO production promoting activity have been searched for, and various NO production promoters have been proposed (Patent Document 1). It is also known that it improves male sexual function by enhancing the NO production promoting effect (Patent Document 2).

[0003] On the other hand, the recent boom in muscle training has led to an increased demand for supplements that claim to maintain muscle after a workout (Non-Patent Document 3). For example, it has been reported that beet juice, which contains nitric oxide (NO), has a muscle maintenance effect via vasodilatory properties (Non-Patent Document 4).

[0004] However, the clinical effects of polyphenols with NO production on muscle recovery after exercise vary. For example, cocoa flavonoids lower blood ROS levels but do not affect NO production (Non-Patent Document 5). Pomegranate polyphenols (gallotannins) increase oxygen uptake (VO2) but do not improve exercise (cycling) performance (Non-Patent Document 6). Resveratrol, known for its anti-arteriosclerosis effect, also has a weak post-workout recovery effect. 19) Furthermore, it has been reported that blackcurrants, which contain a lot of anthocyanins, do not improve oxygen uptake (VO2), but do increase cardiac output (Patent Document 8). As such, even though they are all called polyphenols, there are differences in the post-workout recovery effects in humans.

[0005] [Patent Document 1] JP 2014-001163 A [Patent Document 2] JP 2011-173817 A [Non-Patent Document 1] Nature, 1987, 327, 524-526 [Non-Patent Document 2] Proc. Natl. Acad. Sci. USA, 1987, 84, 9265-9269 [Non-Patent Document 3] Martin JS et al. J. Int. Soc. Sports Nutr. 14, 38 (2017). [Non-Patent Document 4] Richard JC et al. Physiological Rep. 6, e1352 (2018). [Non-Patent Document 5] Decroix L, et al. J. Int. Soc. Sports Nutr. 14:28 (2017). [Non-Patent Document 6] Crum EM, et al. J. Int. Soc. Sports Nutr. 14:14 (2017). [Non-Patent Document 7] Gliemann L. et al. Free Radic. Biol. Med. 98,165-176 (2016). [Non-Patent Document 8] Willems ME, et al, Int. J. Sport. Nutr. Exerc. Metab. 25,367-374 (2015). DISCLOSURE OF THEINVENTION [Problem to be solved by the invention]

[0006] Against this background, the present inventors discovered that purple tea and its inherent component, 1,2-di-O-galloyl-4,6-O-(S)-hexahydroxydiphenoyl-β-D-glucose, have the effect of promoting NO production, and thus completed the present invention. It was also found that purple tea extract has the effect of improving the performance of the lower limb muscles at the start of training, which requires explosive power, and that purple tea extract also has the effect of reducing muscle damage caused by intensive exercise, thus completing the present invention. That is, an object of the present invention is to provide a novel NO production promoter and an agent for improving explosive power in lower limb muscles and an agent for reducing muscle damage associated with intensive exercise using the same. [Means for solving the problem]

[0007] The technical features of the present invention for solving the above problems are as follows. 1. An NO production promoter whose active ingredient is 1,2-di-O-galloyl-4,6-O-(S)-hexahydroxydiphenoyl-β-D-glucose. 2. An NO production promoter comprising a composition containing 1,2-di-O-galloyl-4,6-O-(S)-hexahydroxydiphenoyl-β-D-glucose as an active ingredient. 3. An NO production promoter whose active ingredient is a GHG-containing composition derived from Kenyan purple tea (scientific name: Camellia sinensis, variety name: TRFK306). 4. An agent for improving explosive power in lower limb muscles, containing Kenyan purple tea (scientific name: Camellia sinensis, variety name: TRFK306) extract as the active ingredient. 5. An agent for reducing muscle damage caused by intensive exercise, containing Kenyan purple tea (scientific name: Camellia sinensis, variety name: TRFK306) extract as the active ingredient. [Brief description of the drawings]

[0008] [Figure 1] 1 is an HPLC chromatogram of the Kenyan purple tea extract (purple tea extract) of this example. [Diagram 2] 1 is a graph showing the results of evaluating the NO production promoting effect of purple tea extract. [Diagram 3] 1 is a graph showing the results of evaluation of the NO production promoting effect of GHG. [Figure 4] FIG. 1 is an explanatory diagram of a method for testing the effect of Kenyan purple tea extract on workouts. BEST MODE FOR CARRYING OUT THEINVENTION

[0009] The present invention will be described in detail below. The NO production promoter of the present invention is characterized in that it contains 1,2-di-O-galloyl-4,6-O-(S)-hexahydroxydiphenoyl-β-D-glucose (hereinafter referred to as "GHG") as an active ingredient. GHG is expressed by the following chemical formula (1).

[0010] [ka]

[0011] The NO production promoter of the present invention has a GHG-containing composition as an active ingredient. It is preferable to use a composition derived from Kenyan purple tea (scientific name: Camellia sinensis, variety name: TRFK306) as the GHG-containing composition. The GHG-containing composition is an extract obtained from Kenyan purple tea and contains GHG at a content of 3 to 99% by mass. The form may be liquid, solid, semi-solid, gel, etc., and the solid content of GHG is preferably 3 to 99% by mass, but when used as a material for food, beverage, or cosmetic, the content is preferably 3 to 30% by mass, and more preferably 3 to 10% by mass in order to improve the productivity. In particular, a GHG-containing composition containing GHG at a content of 3 to 10% by mass can be efficiently obtained by the production method described below.

[0012] Kenyan purple tea, the raw material for the GHG-containing composition, is a tea plant developed by the Kenyan government through crossbreeding, and is named TRFK306. Kenyan purple tea leaves contain anthocyanins, which gives them their purple color, and so they are commonly known as "purple tea." In addition to TRFK306, other purple teas include Sunrouge, developed by the National Agriculture and Food Research Organization (NARO), but Kenyan purple tea contains a high concentration of GHG, a unique component not found in other purple teas.

[0013] The part of Kenyan purple tea (hereinafter simply referred to as "purple tea") used for producing the GHG-containing composition is not particularly limited, and leaves, stems, roots, flowers, seeds, etc. can be used, and it is particularly preferable to use leaves, because a higher concentration of GHG can be obtained.

[0014] The GHG-containing composition can be obtained, for example, by crushing raw or dried purple tea leaves (hereinafter referred to as "purple tea leaves") and extracting them with a polar solvent (including water; the same applies below). In order to improve the efficiency of extraction, the purple tea leaves may be subjected to appropriate chemical treatments such as acid or alkali hydrolysis and enzymatic hydrolysis, and then extracted.

[0015] Specifically, the GHG-containing composition can be produced by the method described below. That is, first, as the purple tea leaves, raw or dried purple tea leaves are subjected to a chemical treatment such as acid or alkali hydrolysis or enzymatic hydrolysis.

[0016] Then, a polar solvent is added to the purple tea leaves and the mixture is shaken or heated under reflux to extract the GHGs into the solvent.

[0017] In this case, the polar solvent is not particularly limited, but water, alcohols, and ketones can be used. These may be used alone or in combination of two or more. Among these, it is particularly preferable to use a water-containing alcohol or a water-containing ketone.

[0018] As the hydrous alcohol solvent, hydrous solvents such as ethanol, methanol, and propanol can be used, and hydrous ethanol is particularly preferred.As the hydrous ketone solvent, hydrous solvents such as acetone, methyl ethyl ketone, diethyl ketone, and chloroacetone can be used, and hydrous acetone is particularly preferred.

[0019] In the case of hydrous ethanol, the water content is 1 to 99.9% by mass, preferably 30 to 99% by mass, more preferably 40 to 80% by mass, and most preferably 40 to 60% by mass. In the case of hydrous acetone, it is preferable that the acetone content is 20 to 99.9% by mass. This is because the above range provides excellent GHG extraction efficiency. In the following, for the sake of simplicity, the water content of the hydrous solvent will be expressed as "80% hydrous ethanol", for example, 80% ethanol containing 20% ​​by mass of water.

[0020] In the method for producing the GHG-containing composition, the heating and refluxing can be carried out by a known method using the above-mentioned water-containing alcohol solvent or water-containing ketone solvent. The heating temperature is preferably 30 to 95°C, more preferably about 30 to 50°C, and the reflux time is preferably about 1 to 4 hours.

[0021] In the above-mentioned method for producing a GHG-containing composition, shaking, stirring, etc. may be appropriately performed as necessary.

[0022] In the method for producing the GHG-containing composition, it is preferable to distill off the solvent under reduced pressure after the extraction, which makes it possible to obtain a composition that does not contain an organic solvent and that can comply with safety standards as a food ingredient for incorporation into foods and beverages such as functional foods and health foods.

[0023] Furthermore, in the method for producing the GHG-containing composition, stepwise extraction can be performed with a plurality of solvents, which allows the production of a GHG-containing composition containing a high concentration of GHG with a higher yield.

[0024] Specifically, for example, purple tea leaves are added to either the hydrous alcohol solvent or the hydrous ketone solvent, and shaken or heated to reflux to extract GHG into the solvent, thereby obtaining a first extract. The extract is separated into a residue that was not collected as the extract by centrifugation or the like, and the other of the solvents not selected is added to the residue, and shaken or heated to reflux to extract GHG into the solvent, thereby obtaining a second extract. The first extract and the second extract are then mixed. Needless to say, this second extract can also be used alone as an extract of purple tea leaves (GHG-containing composition).

[0025] In this way, by performing step-by-step extraction using multiple solvents, it is thought that the physical properties and other characteristics of the purple tea leaves will change to those suitable for extraction when they undergo the first extraction process using the above-mentioned hydrous alcoholic solvent or the above-mentioned hydrous ketone solvent, and therefore it is expected that the extraction efficiency will be improved not only when the above-mentioned hydrous alcoholic solvent or the above-mentioned hydrous ketone solvent is used, but also when other solvents are used in the subsequent second extraction process.

[0026] The extract obtained by the above method can be used as a GHG-containing composition as it is or after concentration, and can also be powdered by freeze-drying or spray-drying to provide a GHG-containing composition in powder form. The form is not limited to these. Insoluble matter contained in the extract can be removed by filtration or the like as appropriate. The insoluble matter may be further pulverized into fine particles.

[0027] As a method for producing GHG of the present invention, for example, the GHG-containing composition obtained as described above is preferably fractionated and purified by using known GHG as an index by ion exchange, size exclusion column chromatography, HPLC, gel filtration, membrane separation, etc. Of course, GHG may be extracted and purified from a raw material other than Kenyan purple tea, or an organic synthesis method may be appropriately applied.

[0028] The NO production promoter of the present invention can be utilized in the form of tablets, granules, powders, liquids, powders, granules, capsules, jelly-like agents, etc. (pharmaceuticals or quasi-drugs) by adding a pharmaceutical acceptable base material as necessary to the active ingredient GHG or a GHG-containing composition. In addition, the GHG or GHG-containing composition, which is the active ingredient of the present invention, can be used as a material for various food compositions, beverage compositions, and cosmetic compositions. Note that these compositions refer to products obtained by blending ingredients suitable for each application by some technical means.

[0029] Examples of the form of the food composition and beverage composition according to the present invention include general foods such as edible oils (salad oils), confectioneries (gum, candy, caramel, chocolate, cookies, snacks, jellies, gummies, tablets, etc.), noodles (soba, udon, ramen, etc.), dairy products (milk, ice cream, yogurt, etc.), seasonings (miso, soy sauce, etc.), soups, beverages (juice, coffee, black tea, green tea, carbonated drinks, sports drinks, etc.), health foods (tablets, capsules, etc.), and nutritional supplements (nutritional drinks, etc.). The active ingredient of the present invention (GHG or GHG-containing composition) may be appropriately blended into these food compositions and beverage compositions.

[0030] The food and beverage compositions according to the present invention can contain various ingredients depending on their types, and for example, food ingredients such as glucose, fructose, sucrose, maltose, sorbitol, stevioside, corn syrup, lactose, citric acid, tartaric acid, malic acid, succinic acid, lactic acid, L-ascorbic acid, dl-α-tocopherol, sodium erythorbate, glycerin, propylene glycol, glycerin fatty acid esters, polyglycerin fatty acid esters, sucrose fatty acid esters, sorbitan fatty acid esters, propylene glycol fatty acid esters, gum arabic, carrageenan, casein, gelatin, pectin, agar, B vitamins, nicotinamide, calcium pantothenate, amino acids, calcium salts, colorants, flavorings, and preservatives can be used.

[0031] Furthermore, food compositions and beverage compositions with health-maintaining functions may contain other antioxidants and health food ingredients, (e.g., antioxidants, reduced ascorbic acid (vitamin C), vitamin E, reduced glutathione, tocotrienol, vitamin A derivatives, lycopene, β-cryptoxanthin, astaxanthin, zeaxanthin, fucoxanthin, uric acid, ubiquinone, coenzyme Q10, folic acid, garlic extract, allicin, sezamin, lignans, catechins, isoflavones, chalcones, tannins, flavonoids, coumarins, isocoumarins, blueberry extract, arbutin, tannins, anthocyanins, apple polyphenols, grape seed extract, ellagic acid, kojic acid, surge extract, vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin B12, VC, vitamin D, vitamin K, VE, VP, choline, niacin) , pantothenic acid, calcium folate, EPA, oligosaccharides, dietary fiber, squalene, soy lecithin, taurine, dunaliella, protein, octacosanol, DHA, egg yolk lecithin, linoleic acid, lactoferrin, magnesium, zinc, chromium, selenium, potassium, heme iron, oyster extract, chitosan, chitin oligosaccharides, collagen, chondroitin, turmeric, licorice, wolfberry, cinnamon, hawthorn, ginger, reishi , Shijimi extract, soft-shelled turtle, plantain, chamomile, chamomile, dandelion, hibiscus, honey, bolen, royal jelly, lime, lavender, rose hips, rosemary, sage, bifidobacteria, faecalis bacteria, lacrys, wheat germ oil, sesame oil, perilla oil, soybean oil, medium-chain fatty acids, agaricus, ginkgo leaf extract, turmeric, chondroitin, brown rice germ extract, lychee, onion, DHA, EPA, DPA,Sweet tea, cordyceps, garlic, bee larvae, papaya, pu-erh, propolis, megusuri tree, arbutus mushroom, royal jelly, saw palmetto, hyaluronic acid, collagen, GABA, harp seal oil, shark cartilage, glucosamine, lecithin, phosphatidylserine, Panax notoginseng, mulberry leaves, soybean extract, echinacea, Eleuthero, barley extract, olive leaves, olive fruit, gymnema, banaba, salacia, garcinia, chitosan, St. John's wort, jujube, carrots, passion fruit Flower, broccoli, placenta, pearl barley, grape seeds, peanut husks, bilberry, black cohosh, milk thistle, bay laurel, sage, rosemary, rafuma, black vinegar, bitter melon, maca, safflower, flax, oolong tea, flower thorns, caffeine, capsaicin, xylooligosaccharides, glucosamine, buckwheat, citrus, dietary fiber, protein, prunes, spirulina, young barley leaves, nucleic acid, yeast, shiitake mushrooms, plum flesh, amino acids, deep sea shark extract, noni, oyster meat, soft-shelled turtle, champignon, Plantain, acerola, pineapple, banana, peach, apricot, melon, strawberry, raspberry, orange, fucoidan, maitake mushroom, cranberry, chondroitin sulfate, zinc, iron, ceramide, silk peptide, glycine, niacin, chaste tree, ceramide, L-cysteine, L-carnitine, red wine leaf, millet, horsetail, biotin, centella asiatica, haskap, pycnogenol, butterbur, rhubarb, clove, rosemary, catechin, pu-erh It can also contain the following ingredients: citric acid, brewer's yeast, melilot, black ginger, ginger, zedoary, nattokinase, monascus, tocotrienol, lactoferrin, cinnamon, tartary buckwheat, cocoa, yuzu seed extract, perilla fruit extract, lychee seed extract, evening primrose extract, black rice extract, alpha-lipoic acid, GABA, green coffee bean extract, butterbur extract, kiwi seed extract, Satsuma mandarin extract, red ginger extract, astaxanthin, nattokinase, and maca extract.

[0032] As a specific manufacturing method, the active ingredient of the present invention (GHG or GHG-containing composition) can be spray-dried or freeze-dried as it is, or in the case of an extract, together with powdered dextrin, and then made into a powder, granules, tablet, or solution, which can be easily incorporated into foods (instant foods, etc.). If necessary, it can also be mixed with a binder such as gum arabic to form a powder or granules, which can then be added to solid foods.

[0033] When the NO production promoter of the present invention is in the form of a drug (including pharmaceuticals and quasi-drugs), it can be produced by appropriately mixing the active ingredient of the present invention (GHG or a GHG-containing composition) with the raw material for pharmaceutical preparations. The above-mentioned drug may be used for humans or for organisms other than humans (such as mammals). Examples of pharmaceutical raw materials that can be incorporated into the above-mentioned drugs include excipients (glucose, lactose, sucrose, sodium chloride, starch, calcium carbonate, kaolin, crystalline cellulose, cacao butter, hardened vegetable oil, kaolin, talc, etc.), binders (distilled water, physiological saline, ethanol water, simple syrup, glucose solution, starch solution, gelatin solution, carboxymethylcellulose, potassium phosphate, polyvinylpyrrolidone, etc.), disintegrants (sodium alginate, agar, sodium bicarbonate, calcium carbonate, sodium lauryl sulfate, stearate monoglyceride, starch, lactose, powdered gum arabic, gelatin, ethanol, etc.), disintegration inhibitors (sucrose, stearin, cacao butter, hydrogenated oil, etc.), absorption enhancers (quaternary ammonium base, sodium lauryl sulfate, etc.), adsorbents (glycerin, starch, lactose, kaolin, bentonite, silicic acid, etc.), lubricants (purified talc, stearates, polyethylene glycol, etc.), etc.

[0034] The above-mentioned drugs can generally be administered orally in the form of tablets, pills, soft or hard capsules, fine granules, powders, granules, etc. Water-soluble preparations can be administered orally as liquids. They may also be administered parenterally. When administered parenterally, the present composition, etc. can be dispersed in a suitable solubilizing agent such as ethanol or water, and then applied in the form of poultices, lotions, ointments, tinctures, creams, etc. Water-soluble preparations such as the present composition, etc., can be applied as they are or in the form of poultices, lotions, ointments, tinctures, creams, etc., with the addition of a dispersing agent, a suspending agent, a stabilizer, etc.

[0035] The dosage may vary depending on the method of administration, the condition, the age of the patient, etc., but typically, for adults, 5 to 200 mg of the active ingredient can be administered per day, and for children, 0.5 to 100 mg.

[0036] The compounding ratio of the active ingredient (GHG or GHG-containing composition) of the present invention when used as the above-mentioned drug can be appropriately changed depending on the dosage form, but it is usually about 0.01 to 10 wt% when administered orally or by mucosal absorption, and about 0.01 to 20 wt% when administered parenterally. Since the dosage varies depending on various conditions, a smaller amount than the above dosage may be sufficient, or it may be necessary to administer it beyond the range. In addition to the present composition, the pharmaceutical composition may contain other known compounds commonly used in the pharmaceutical field and compounds necessary for molding into a form suitable for oral administration. Examples of such compounds include lactose, starch, hydroxypropyl cellulose, kaolin, talc, calcium carbonate, etc. In addition, sildenafil and its derivatives, etc. may be appropriately blended.

[0037] Furthermore, the present invention can also be used as a cosmetic. Examples of the form of the cosmetic composition of the present invention include milky lotion, soap, facial cleanser, bath agent, cream, milky lotion, skin lotion, cologne, shaving cream, shaving lotion, cosmetic oil, sunscreen lotion, face powder, foundation, perfume, pack, nail cream, enamel, enamel remover, eyebrow ink, blusher, eye cream, eye shadow, mascara, eyeliner, lipstick, lip balm, shampoo, rinse, hair dye, dispersion, cleanser, etc.

[0038] In addition to the active ingredient of the present invention (GHG or GHG-containing composition), the cosmetic preparation in the above form may contain ingredients that are blended into cosmetics, external preparations for skin such as quasi-drugs, oils, higher alcohols, fatty acids, ultraviolet absorbers, powders, pigments, surfactants, polyhydric alcohols / sugars, polymers, physiologically active ingredients, solvents, antioxidants, fragrances, preservatives, etc., within a range that does not impair the action and effect of the cosmetic preparation. Examples are listed below, but the present invention is not limited to these examples.

[0039] (1) Example of oil Ester-based oil phase components: glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, butyl stearate, butyl myristate, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, diisopropyl adipate isopropyl, isoarachyl neopentanoate, tri(caprylic / capric)glyceryl, trimethylolpropane tri-2-ethylhexanoate, trimethylolpropane triisostearate, pentaerythritol tetra-2-ethylhexanoate, cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinoleate, isostearyl laurate, isotridecyl myristate, isocetyl myristate, isostearate Tearyl, isocetyl palmitate, isostearyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, cetostearyl 2-ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, propylene glycol dicaprylate, propylene glycol dicaprylate, dicaprin Neopentyl glycol acid, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerin oleate, polyglycerin isostearate, dipropyl carbonate,Dialkyl carbonate (C12-18), triisocetyl citrate, triisoarachyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate propyl, dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, phytosteryl isostearate, phytosteryl oleate, isocetyl 12-stearoylhydroxystearate, stearyl 12-stearoylhydroxystearate, isostearyl 12-stearoylhydroxystearate, and the like. Hydrocarbon-based oil phase components: squalane, liquid paraffin, α-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, petrolatum, etc. Animal and vegetable oils and their hydrogenated oils, and waxes of natural origin: beef tallow, hydrogenated beef tallow, lard, hydrogenated lard, horse oil, hydrogenated horse oil, mink oil, orange roughy oil, fish oil, hydrogenated fish oil, egg yolk oil, and other animal oils and their hydrogenated oils, avocado oil, almond oil, olive oil, cocoa butter, kiwi seed oil, apricot kernel oil, coconut oil, sesame oil, wheat germ oil, rice germ oil, rice bran oil, safflower oil, shea butter, soybean oil, evening primrose oil, perilla oil, tea seed oil, camellia oil, corn oil, rapeseed oil, hard Examples of such vegetable oils include hydrogenated rapeseed oil, palm kernel oil, hydrogenated palm kernel oil, palm oil, hydrogenated palm oil, peanut oil, hydrogenated peanut oil, castor oil, hydrogenated castor oil, sunflower oil, grape seed oil, jojoba oil, hydrogenated jojoba oil, macadamia nut oil, medicamentoil, cottonseed oil, hydrogenated cottonseed oil, coconut oil, and hydrogenated coconut oil, as well as their hydrogenated oils, beeswax, high acid value beeswax, lanolin, reduced lanolin, hydrogenated lanolin, liquid lanolin, carnauba wax, and montan wax. Silicone-based oil phase components: dimethylpolysiloxane, methylphenylpolysiloxane, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, methylhydrogenpolysiloxane, polyether-modified organopolysiloxane, dimethylsiloxane-methylcetyloxysiloxane copolymer, dimethylsiloxane-methylstearoxysiloxane copolymer, alkyl-modified organopolysiloxane, terminal-modified organopolysiloxane, amino-modified silicone oil, amino-modified organopolysiloxane, dimethiconol, silicone gel, acrylic silicone, trimethylsiloxysilicate, silicone RTV rubber, etc. Fluorine-based oil phase components: Perfluoropolyether, fluorine-modified organopolysiloxane, fluorinated pitch, fluorocarbon, fluoroalcohol, fluoroalkyl-polyoxyalkylene-co-modified organopolysiloxane, etc.

[0040] (2) Examples of higher alcohols Examples of the alcohol include lauryl alcohol, myristyl alcohol, cetyl alcohol, stearyl alcohol, isostearyl alcohol, oleyl alcohol, behenyl alcohol, 2-ethylhexanol, hexadecyl alcohol, and octyldodecanol.

[0041] (3) Examples of fatty acids Caprylic acid, capric acid, Examples of the acid include undecylenic acid, lauric acid, myristic acid, palmitic acid, palmitoleic acid, stearic acid, isostearic acid, oleic acid, linoleic acid, linolenic acid, arachic acid, arachidonic acid, behenic acid, erucic acid, and 2-ethylhexanoic acid.

[0042] (4) Examples of UV absorbers Para-aminobenzoic acid, amyl para-aminobenzoate, dihydroxypropyl ethyl para-aminobenzoate, glyceryl para-aminobenzoate, ethyl para-aminobenzoate, octyl para-aminobenzoate, octyldimethyl para-aminobenzoate, ethylene glycol salicylate, octyl salicylate, triethanolamine salicylate, phenyl salicylate, butylphenyl salicylate, benzyl salicylate, homomenthyl salicylate, benzyl cinnamate, octyl para-methoxycinnamate, 2-ethylhexyl para-methoxycinnamate, glyceryl di-para-methoxycinnamate mono 2-ethylhexanoate, isopropyl para-methoxycinnamate, diethanolamine salt of para-methoxyhydrocinnamic acid, mixture of diisopropyl and diisopropyl cinnamate, urocanic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and its salts, Examples of the suitable benzotriazoles include dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzophenone, dihydroxydimethoxybenzophenone, hydroxyoctoxybenzophenone, tetrahydroxybenzophenone, butylmethoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2-ethylhexyl-1-oxy)-1,3,5-triazine, 2-(2-hydroxy-5-methylphenyl)benzotriazole, methyl-O-aminobenzoate, 2-ethylhexyl-2-cyano-3,3-diphenylacrylate, phenylbenzimidazole sulfate, 3-(4-methylbenzylidene)camphor, isopropyldibenzoylmethane, 2-ethylhexyl 4-(3,4-dimethoxyphenylmethylene)-2,5-dioxo-1-imidazolidinepropionate, and the like, as well as polymer derivatives and silane derivatives thereof.

[0043] (5) Examples of powders and pigments Dyes such as Red No. 104, Red No. 201, Yellow No. 4, Blue No. 1, Black No. 401, lake dyes such as Yellow No. 4 AL Lake, Yellow No. 203 BA Lake, nylon powder, silk powder, urethane powder, Teflon (registered trademark) powder, silicone powder, polymethyl methacrylate powder, cellulose powder, starch, silicone elastomer spherical powder, polyethylene powder and other polymers, yellow iron oxide, red iron oxide, black iron oxide, chromium oxide, carbon black, ultramarine, Prussian blue and other colored pigments, zinc oxide, titanium oxide, acid Examples of such pigments include white pigments such as cerium chloride, extender pigments such as talc, mica, sericite, kaolin, and plate-like barium sulfate, pearl pigments such as titanium mica, metal salts such as barium sulfate, calcium carbonate, magnesium carbonate, aluminum silicate, and magnesium silicate, inorganic powders such as silica and alumina, metal soaps such as aluminum stearate, magnesium stearate, zinc palmitate, zinc myristate, magnesium myristate, zinc laurate, and zinc undecylenate, bentonite, smectite, and boron nitride. There are no particular limitations on the shape (spherical, rod-like, needle-like, plate-like, irregular, flaky, spindle-like, etc.) and particle size of these powders. These powders may or may not have been previously surface-treated by a conventionally known surface treatment, such as a fluorine compound treatment, a silicone treatment, a silicone resin treatment, a pendant treatment, a silane coupling agent treatment, a titanium coupling agent treatment, an oil treatment, an N-acylated lysine treatment, a polyacrylic acid treatment, a metal soap treatment, an amino acid treatment, a lecithin treatment, an inorganic compound treatment, a plasma treatment, or a mechanochemical treatment.

[0044] (6) Examples of surfactants Anionic surfactants: fatty acid soaps, α-acylsulfonates, alkylsulfonates, alkylarylsulfonates, alkylnaphthalenesulfonates, alkyl sulfates, POE alkyl ether sulfates, alkylamide sulfates, alkyl phosphates, POE alkyl phosphates, alkylamide phosphates, alkyloylalkyl taurine salts, N-acylamino acid salts, POE alkyl ether carboxylates, alkylsulfosuccinates, sodium alkylsulfoacetates, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, and the like. Cationic surfactants: alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, benzalkonium chloride, behenic acid amidopropyldimethylhydroxypropylammonium chloride, stearate diethylaminoethylamide, stearate dimethylaminopropylamide, lanolin derivative quaternary ammonium salts, etc. Amphoteric surfactants: Examples of amphoteric surfactants include carboxybetaine type, amidobetaine type, sulfobetaine type, hydroxysulfobetaine type, amidosulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type, and amidoamine type. Nonionic surfactants: Propylene glycol fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, sorbitan fatty acid esters, POE sorbitan fatty acid esters, POE sorbit fatty acid esters, POE glycerin fatty acid esters, POE alkyl ethers, POE fatty acid esters, POE hydrogenated castor oil, POE castor oil, POE·POP copolymers, POE·POP alkyl ethers, polyether modified silicone lauric acid alkanolamide, alkylamine oxide, hydrogenated soybean phospholipids, etc. Natural surfactants: Examples include lecithin, saponin, sugar-based surfactants, etc.

[0045] (7) Examples of polyhydric alcohols and sugars Examples of suitable sugars include ethylene glycol, diethylene glycol, polyethylene glycol, propylene glycol, dipropylene glycol, polypropylene glycol, glycerin, diglycerin, polyglycerin, 3-methyl-1,3-butanediol, 1,3-butylene glycol, sorbitol, mannitol, raffinose, erythritol, glucose, sucrose, fructose, xylitol, lactose, maltose, maltitol, trehalose, alkylated trehalose, mixed isomerized sugar, sulfated trehalose, pullulan, etc. Chemically modified versions of these sugars may also be used.

[0046] (8) Examples of polymers Acrylic acid ester / methacrylic acid ester copolymer (Plussize, manufactured by GOO Chemical Co., Ltd.), vinyl acetate / crotonic acid copolymer (Resin 28-1310, manufactured by NSC Co., Ltd.), vinyl acetate / crotonic acid / vinyl neodecanoate copolymer (28-2930, manufactured by NSC Co., Ltd.), methyl vinyl ether maleic acid half ester (Gantrez ES, manufactured by ISP Co., Ltd.), t-butyl acrylate / ethyl acrylate / methacrylic acid copolymer (Rubymer, manufactured by BASF Co., Ltd.), vinyl pyrrolidone / vinyl acetate / vinyl propionate copolymer (Ruviscol VAP, manufactured by BASF Co., Ltd.), vinyl acetate Vinyl acetate / crotonic acid copolymer (Rubyset CA, BASF), vinyl acetate / crotonic acid / vinyl pyrrolidone copolymer (Rubyset CAP, BASF), vinyl pyrrolidone / acrylate copolymer (Rubyflex, BASF), acrylate / acrylamide copolymer (Ultrahold, BASF), vinyl acetate / butyl maleate / isobornyl acrylate copolymer (Advantage, ISP), carboxyvinyl polymer (Carbopol, BFGoodrich), acrylic acid / alkyl methacrylate copolymer (Pemulen, BF Examples of such polymers include anionic polymers such as acetic acid amphoteric product of dialkylaminoethyl methacrylate polymer (Yukaformer, manufactured by Mitsubishi Chemical Corporation) and amphoteric polymers such as octylacrylamide acrylate / hydroxypropyl acrylate / butylaminoethyl methacrylate copolymer (AMPHOMER, manufactured by NSC Corporation); cationic polymers such as quaternary product of vinylpyrrolidone / dimethylaminoethyl methacrylate (GAFQUAT, manufactured by ISP Corporation) and methylvinylimidazolium chloride / vinylpyrrolidone copolymer (Rubycoat, manufactured by BASF Corporation); and nonionic polymers such as polyvinylpyrrolidone (Ruviscol K, manufactured by BASF Corporation), vinylpyrrolidone / vinyl acetate copolymer (Ruviscol VA, manufactured by BASF Corporation), vinylpyrrolidone / dimethylaminoethyl methacrylate copolymer (copolymer 937, manufactured by ISP Corporation), and vinylcaprolactam / vinylpyrrolidone / dimethylaminoethyl methacrylate copolymer (copolymer VC713, manufactured by ISP Corporation).Also suitable for use are naturally occurring polymeric compounds such as cellulose or derivatives thereof, keratin and collagen or derivatives thereof, calcium alginate, pullulan, agar, gelatin, tamarind seed polysaccharides, xanthan gum, carrageenan, high methoxyl pectin, low methoxyl pectin, guar gum, gum arabic, crystalline cellulose, arabinogalactan, karaya gum, tragacanth gum, alginic acid, albumin, casein, curdlan, gellan gum, and dextran.

[0047] (9) Examples of physiologically active ingredients Examples of the physiologically active ingredient include substances that impart some physiological activity to the skin when applied to the skin. For example, they include whitening ingredients, immune activators, anti-aging agents, UV protection agents, slimming agents, tightening agents, antioxidants, hair growth agents, hair restorers, moisturizers, blood circulation promoters, antibacterial agents, bactericides, drying agents, cooling agents, warming agents, vitamins, amino acids, wound healing promoters, irritation relievers, analgesics, cell activators, enzyme ingredients, etc. Examples of suitable ingredients include angelica extract, avocado extract, amacha extract, althea extract, arnica extract, aloe extract, apricot extract, apricot kernel extract, ginkgo extract, fennel extract, turmeric extract, oolong tea extract, eijitsu extract, echinacea leaf extract, scutellaria extract, phellodendron bark extract, coptis japonica extract, barley extract, hypericum extract, white nettle extract, watercress extract, orange extract, Dried seawater, seaweed extract, hydrolyzed elastin, hydrolyzed wheat powder, hydrolyzed silk, chamomile extract, carrot extract, artemisia capillaris extract, licorice extract, kalkade extract, kakyoku extract, cinchona extract, cucumber extract, guanosine, gardenia extract, kumazasa extract, sophora flavescens extract, walnut extract, grapefruit extract, clematis extract, chlorella extract, mulberry extract, gentiana extract, black tea extract, yeast extract, burdock extract, coix seed extract, Fermented rice bran extract, rice germ oil, comfrey extract, collagen, lingonberry extract, Chinese ivy extract, Chinese rhizome extract, umbilical cord extract, salvia extract, soapwort extract, bamboo extract, hawthorn extract, Japanese pepper extract, shiitake mushroom extract, rehmannia extract, lithospermum extract, perilla extract, linden extract, meadowsweet extract, peony extract, calamus root extract, white birch extract, horsetail extract, ivy extract, hawthorn extract, se Elderberry extract, yarrow extract, peppermint extract, sage extract, mallow extract, cnidium extract, swertia britannica extract, soybean extract, tsinga extract, thyme extract, tea extract, clove extract, imperata cylindrica extract, tangerine peel extract, angelica extract, calendula extract, peach kernel extract, spruce extract, houttuynia cordata extract, tomato extract, natto extract, carrot extract, garlic extract, wild rose extract, hibiscus extract,Examples of such extracts include burdock root extract, parsley extract, honey, witch hazel extract, parietaria extract, burdock root extract, bisabolol, loquat extract, coltsfoot extract, butterbur bud extract, poria extract, butcher's broom extract, grape extract, propolis, loofah extract, safflower extract, peppermint extract, linden extract, peony extract, hop extract, pine extract, horse chestnut extract, skunk cabbage extract, soapberry extract, melissa extract, peach extract, cornflower extract, eucalyptus extract, saxifrage extract, coix seed extract, mugwort extract, lavender extract, apple extract, lettuce extract, lemon extract, astragalus extract, rose extract, rosemary extract, Roman chamomile extract, and royal jelly extract. In addition, biopolymers such as sildenafil and its derivatives, deoxyribonucleic acid, mucopolysaccharides, sodium hyaluronate, sodium chondroitin sulfate, collagen, elastin, chitin, chitosan, and hydrolyzed eggshell membrane, moisturizing ingredients such as amino acids, hydrolyzed peptides, sodium lactate, urea, sodium pyrrolidone carboxylate, betaine, whey, and trimethylglycine, oily ingredients such as sphingolipids, ceramide, phytosphingosine, cholesterol, cholesterol derivatives, and phospholipids, ε-aminocaproic acid, glycyrrhizic acid, β-glycyrrhetin, and the like. Immune stimulants such as tin acid, lysozyme chloride, guaiazulene, and hydrocortisone; vitamins such as vitamin A, vitamin B2, vitamin B6, vitamin C, vitamin D, vitamin E, calcium pantothenate, biotin, nicotinamide, and vitamin C ester; active ingredients such as allantoin, diisopropylamine dichloroacetate, and 4-aminomethylcyclohexanecarboxylic acid; antioxidants such as tocopherol, carotenoids, flavonoids, tannins, lignans, and saponins; cell stimulants such as α-hydroxy acids and β-hydroxy acids; and γ-oryza sativa. blood circulation promoters such as ethanol and vitamin E derivatives, wound healing agents such as retinol and retinol derivatives, skin whitening agents such as arbutin, kojic acid, placenta extract, sulfur, ellagic acid, linoleic acid, tranexamic acid, and glutathione, cepharanthine, licorice extract, capsicum tincture, hinokitiol, iodized garlic extract, pyridoxine hydrochloride, DL-α-tocopherol, DL-α-tocopherol acetate, nicotinic acid, nicotinic acid derivatives, calcium pantothenate, D-pantothenyl alcohol, acetyl pantothenyl ethyl ether, biotin, alanine Toin, isopropyl methylphenol, estradiol, ethinyl estradiol, capronium chloride, benzalkonium chloride, diphenhydramine hydrochloride, tacanal, camphor, salicylic acid, vanillylamide nonylate, vanillylamide nonanoate, piroctone olamine, glyceryl pentadecanoate, L-menthol, mononitroguaiacol, resorcinol, gamma-aminobutyric acid, benzethonium chloride, mexiletine hydrochloride, auxin, female hormones, cantharides tincture, cyclosporine, zinc pyrithione, hydrocortisone, minoxidil,Examples of hair growth agents include polyoxyethylene sorbitan monostearate, peppermint oil, Sasanishiki extract, placenta, yuzu seed extract, blueberry extract, lingonberry extract, Cistanche Tubulosa extract, black rice extract, green coffee bean extract, resveratrol, kiwi seed extract, strawberry seed extract, and cherry blossom extract.

[0048] (10) Examples of antioxidants Examples of antioxidants include sodium bisulfite, sodium sulfite, erythorbic acid, sodium erythorbate, dilauryl thiodipropionate, tocopherol, tolylbiguanide, nordihydroguaiaretic acid, parahydroxyanisole, butylhydroxyanisole, dibutylhydroxytoluene, ascorbyl stearate, ascorbyl palmitate, octyl gallate, propyl gallate, carotenoids, flavonoids, tannins, lignans, saponin, and plant extracts such as apple extract and clove extract that have antioxidant effects.

[0049] The agent for improving the explosive power of lower limb muscles and the agent for reducing muscle damage caused by intensive exercise of the present invention are characterized by containing the above-mentioned purple tea extract as an active ingredient. The above-mentioned purple tea extract can be obtained by the same method as the method for obtaining the above-mentioned GHG-containing composition, and can also be used in medicines (including pharmaceuticals and quasi-drugs), food compositions, beverage compositions, and cosmetics by the same method as the GHG-containing composition. EXAMPLES

[0050] The following describes specific examples of the present invention, but the present invention is not limited to the following examples. Example: Preparation of purple tea extract (GHG-containing composition) 50g of purple tea leaves were immersed in 500mL of 50% ethanol aqueous solution and extracted by heating and refluxing at 40℃ for 2 hours with stirring. 400mL of extract was obtained by suction filtration. The extract was concentrated and dried to obtain 16.6g of purple tea extract.

[0051] Isolation of GHG from purple tea extract When purple tea extract was subjected to HPLC analysis under the conditions described below, a peak was confirmed at 27.5 minutes for a component unique to purple tea extract that is not found in common teas such as green tea, oolong tea, or black tea (indicated by the arrow in Figure 1). Sample preparation: 350 mg of purple tea extract was dissolved in 30% methanol aqueous solution and the volume was adjusted to 20 mL in a measuring flask. The solution was diluted 2-fold, filtered, and then subjected to HPLC analysis. The HPLC analysis conditions were as follows: HPLC analysis conditions Flow rate: 0.7 mL / min Mobile phase A: 0.3% TFA aqueous solution Mobile phase B: Acetonitrile Gradient: As shown in Table 1 below Column: SunFire C18, 4.6×150 mm (Waters) or equivalent Column temperature: 30℃ Sample injection volume: 10 μL Detection wavelength: 280 nm

[0052] [Table 1]

[0053] The specific component was separated and purified and analyzed by NMR. The results are shown in Table 2. As a result of Table 2, it was identified as the known component 1,2-di-O-galloyl-4,6-O-(S)-hexahydroxydiphenoyl-β-D-glucose (GHG).

[0054] [Table 2]

[0055] Quantitative analysis by HPLC using the GHG purified product as a standard substance revealed that the purple tea extract contained 8.70% GHG. The purple tea extract was prepared twice more in the same manner as above, and the GHG content in the extract was measured in the same manner, resulting in 6.79% and 6.38%, respectively. This confirmed that the purple tea extract produced by the method of this example contains approximately 6-9% GHG.

[0056] Test Example 1: Evaluation of NO production promoting activity of purple tea extract and GHG The purple tea sample and GHG sample obtained in the above examples were dissolved in a medium containing 1% DMSO (concentration: 10 to 1000 μg / mL) to prepare a sample solution. A medium containing 1% DMSO was used as a control. Human umbilical vein endothelial cells (HUVECs) (6.2 × 10 4 The cells (cells / 500 μL) were seeded on a 24-well plate and pre-cultured for 1 day, after which the medium was replaced (450 μL). Then, 50 μL of the sample solution was added to each well and reacted for 24 hours. After the reaction, the culture supernatant was used to measure the amount of NO production. 2 / NO 3 The results are shown in Figure 2 (purple tea extract) and Figure 3 (GHG).

[0057] Results and Effects of the Examples in Test Example 1 No purple tea extract 2- ,NO 3- GHGs were found to have a positive effect on the production of NO and total NO. 3- and the effect of promoting the production of total NO was observed. From the above, it was confirmed that purple tea extract and GHG are useful as NO production promoters.

[0058] Test Example 2: Effects on Workout The study was conducted in a randomized, placebo-controlled, crossover format on 32 healthy American males who performed resistance training once or twice a week. The subjects' average age was 33.5 years, and their weight and BMI were typical of American builds. The subjects were asked to take a placebo capsule or a capsule containing purple tea extract (100 mg) once a day in the morning, and on the fifth and eighth days of intake, they performed bench presses (load equivalent to body weight) and leg extensions (load 50% of body weight) to their limits in two sets each. After a two-week recovery period, the subjects were asked to take a different capsule from the first half and underwent the same test. Blood samples were taken before and after exercise, and various parameters were measured (Figure 4). The results are shown in Table 3 (exercise performance after intervention), Table 4 (changes in muscle damage and inflammatory markers), and Table 5 (myoglobin and oxygen saturation after leg extension).

[0059] [Table 3]

[0060] [Table 4]

[0061] [Table 5]

[0062] Results and Effects of the Examples in Test Example 2 The results of the test showed that there was no significant difference between the placebo group and the purple tea group in bench press repetitions, but the number of successful attempts in the first set of leg extensions increased significantly in the purple tea group on the eighth day of intake (Table 3). No increasing effect of the purple tea extract was observed in the second set of leg extensions or the total number of successful attempts. Regarding blood parameters, creatine kinase, which escapes from skeletal muscle, increased in both groups from the 5th to 8th days of intake, but no inhibition was observed by purple tea extract (Table 4). In addition, no change was observed in the inflammatory marker CRP, but LDH levels, which escape due to cell damage, significantly decreased on the 8th day of intake of purple tea extract compared to the placebo group. On the other hand, myoglobin, a pigment protein in muscle, showed almost no change after leg extension (Table 5), but oxygen saturation on the 5th day of intervention significantly decreased in the purple tea extract group 30 and 60 seconds after leg extension. These results revealed that purple tea extract improves the performance of lower limb muscles at the start of training, which requires explosive power. This confirmed that purple tea extract is useful as an agent for improving explosive power in lower limb muscles. In addition, a decrease in blood LDH was observed at this time, confirming that purple tea extract reduced muscle damage associated with intensive exercise, and thus confirmed that purple tea extract is useful as an agent for reducing muscle damage associated with intensive exercise.

[0063] Examples of combinations of the NO production promoter, the agent for improving explosive power in lower limb muscles, and the agent for reducing muscle damage associated with intensive exercise (purple tea extract) of the present invention are given below, but the following examples are not intended to limit the present invention. Formulation example 1: Chewing gum Sugar 53.0wt% Gum base 20.0 Glucose 10.0 Syrup 16.0 Fragrance 0.5 Purple tea extract 0.5 100.0wt%

[0064] Mixture Example 2: Gummies Reduced starch syrup 40.0wt% Granulated sugar 20.0 Glucose 20.0 Gelatin 4.7 Wednesday 9.68 Kiwi juice 4.0 Kiwi flavor 0.6 Dye 0.02 Purple Tea Extract 1.0 100.0wt%

[0065] Mixture example 3: Candy Sugar 50.0wt% Syrup 33.0 Wednesday 14.4 organic acid 2.0 Fragrance 0.2 Purple tea extract 0.4 100.0wt%

[0066] Mixture example 4: Yogurt (hard / soft) Milk 41.5wt% Skim milk powder 5.8 Sugar 8.0 Agar 0.15 Gelatin 0.1 Lactic acid bacteria 0.005 Purple tea extract 0.4 fragrance trace amount water residue 100.0wt%

[0067] Formulation example 5: Soft drink Fructose glucose liquid sugar 30.0wt% Emulsifier 0.5 Purple tea extract 0.05 Fragrance (appropriate amount) Purified water remainder 100.0wt%

[0068] Formulation example 6: Soft capsule Rice germ oil 87.0wt% Emulsifier 12.0 Purple Tea Extract 1.0 100.0wt%

[0069] Formulation Example 7: Tablets Lactose 54.0wt% Crystalline cellulose 30.0 Starch decomposition product 10.0 Glycerol fatty acid ester 5.0 Purple Tea Extract 1.0 100.0wt%

[0070] Formulation Example 8: Granules for oral administration (pharmaceutical) Purple tea extract 1.0wt% Lactose 30.0 Cornstarch 60.0 Microcrystalline cellulose 8.0 Polyvinylpyrrolidone 1.0 100.0wt%

[0071] Formulation example 9: Tablets (medicine) Sugar 76.4wt% Glucose 19.0 Sucrose fatty acid ester 0.2 Purple tea extract 0.5 Purified water 3.9 100.0wt%

[0072] Example 15: Cat food Corn 34.0wt% Wheat flour 35.0 Meat meal 15.0 Beef tallow 8.9 Salt 1.0 Bonito extract 4.0 Purple Tea Extract 1.0 Taurine 0.1 Vitamins 0.5 Minerals 0.5 100.0wt%

[0073] Formulation example 16: Dog food Corn 30.0wt% Meat (chicken) 15.0 Defatted soybeans 10.0 Wheat flour 25.0 Bran 5.0 Purple Tea Extract 5.0 Animal fats and oils 8.9 Oligosaccharides 0.1 Vitamin 0.5 Minerals 0.5 100.0wt% [Industrial Applicability]

[0074] INDUSTRIAL APPLICABILITY As described above, the present invention can provide an NO production promoter that is safe and has few side effects, an agent for improving explosive power in lower limb muscles, and an agent for reducing muscle damage associated with intensive exercise.

Claims

[Claim 1] An agent for lowering blood LDH levels after intensive exercise, containing Kenyan purple tea (scientific name: Camellia sinensis, cultivar name: TRFK306) extract as an active ingredient, An agent for lowering blood LDH levels after intensive exercise, comprising taking 100 mg of Kenyan purple tea (scientific name: Camellia sinensis, variety name: TRFK306) extract once a day for eight days.

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