Kampo medicine

Abstract

To provide a Kampo preparation that contains seisin-rensi-in extract containing glycyrrhizinic acid of 0.22 wt.% or more, in the preparation of which a discomfort sweetness peculiar to glycyrrhizinic acid is reduced.SOLUTION: The present invention discloses a Kampo preparation that contains seisin-rensi-in extract containing glycyrrhizinic acid of 0.22 wt.% or more. The seisin-rensi-in extract is prepared so that relative to 100 pts.wt. of glycyrrhizinic acid, the content of ononin is 8 pts.wt. or more, resulting in reduced sweetness of glycyrrhizinic acid.SELECTED DRAWING: None

Description

Technical Field

[0001] The present invention relates to a Chinese herbal preparation containing Coptis and Lotus Seed Decoction extract.

Background Art

[0002] Coptis and Lotus Seed Decoction extract is an extract of a crude drug mixture containing 9 kinds of crude drugs, and is used for the following various symptoms: a feeling of incomplete urination, frequent urination, and painful urination, although there is general fatigue, dry mouth and tongue, and excessive urination (Non-Patent Documents 1, 2).

[0003] As a method for producing Coptis and Lotus Seed Decoction extract, a method of decocting a crude drug mixture with water (Non-Patent Documents 1, 2) is generally used.

[0004] Many pharmacological actions of glycyrrhizic acid contained in licorice used in the production of Coptis and Lotus Seed Decoction extract, such as anti-inflammatory action, immunomodulatory action, and hepatocyte proliferation action, have been reported (Non-Patent Document 3). In addition, glycyrrhizic acid is known to have a sweetness about 170 times that of sugar (Non-Patent Document 4).

Prior Art Documents

Non-Patent Documents

[0005]

Non-Patent Document 1

Non-Patent Document 2

Non-Patent Document 3

Non-Patent Document 4

Summary of the Invention

Problems to be Solved by the Invention

[0006] If the glycyrrhizic acid derived from licorice contained in the Hoshinrenjiin extract increases, it is considered that the pharmacological action of this component can be enjoyed more effectively. Even with the manufacturing methods commonly used industrially, depending on the component content in licorice, etc., a Hoshinrenjiin extract with a high content of glycyrrhetinic acid may be obtained. However, as a result of the study by the present inventors, when the glycyrrhizic acid in the Hoshinrenjiin extract becomes 0.22% by weight or more, the sweetness of this component is too strong, and the Hoshinrenjiin extract preparation exhibits a peculiar unpleasant sweetness.

[0007] Therefore, an object of the present invention is to provide a formulation for suppressing the peculiar unpleasant sweetness of glycyrrhizic acid in a Kampo preparation containing a Hoshinrenjiin extract containing 0.22% by weight or more of glycyrrhizic acid.

Means for Solving the Problems

[0008] The present inventors changed the ratios of various components contained in the Hoshinrenjiin extract by an original manufacturing method, and found that by adjusting the content of ononin to 8 parts by weight or more with respect to 100 parts by weight of glycyrrhizic acid, the peculiar unpleasant sweetness of glycyrrhizic acid is suppressed. The present invention was completed by further studies based on this finding.

[0009] That is, the present invention provides an invention in the following aspects. Item 1. A Kampo preparation containing a Hoshinrenjiin extract, wherein in the Hoshinrenjiin extract, the content of glycyrrhizic acid is 0.22% by weight or more, and the content of ononin with respect to 100 parts by weight of glycyrrhizic acid is 8 parts by weight or more. Item 2. The Kampo preparation according to Item 1, which is a powder, fine granule, granule, troche, or chewable agent. Item 3. A method for suppressing the bitterness of glycyrrhizic acid in a Kampo preparation containing a Qingxin Lianziyin extract containing 0.22% by weight or more of glycyrrhizic acid, A method of adjusting the content of ononin to 8 parts by weight or more with respect to 100 parts by weight of glycyrrhizic acid in the Qingxin Lianziyin extract.

Advantages of the Invention

[0010] According to the present invention, in a Kampo preparation containing a Qingxin Lianziyin extract containing 0.22% by weight or more of glycyrrhizic acid, it is possible to suppress the peculiar unpleasant bitterness of glycyrrhizic acid.

Modes for Carrying Out the Invention

[0011] 1. Herbal preparation The Kampo preparation of the present invention is characterized by containing a Qingxin Lianziyin extract in which specific components are contained in specific ratios. Hereinafter, the Kampo preparation of the present invention will be described in detail.

[0012] Qingxin Lianziyin is described in the medical book "Wanbing Huichun" of the Ming Dynasty in China and is a mixed crude drug containing Rehmanniae Radix, Scutellariae Radix, Plantaginis Semen, Radix Glehniae, Schisandrae Chinensis Fructus, Coptidis Rhizoma, Glycyrrhizae Radix, Gardeniae Fructus, and Paeoniae Radix Alba.

[0013] In the present invention, the mixing ratio of the crude drugs constituting Qingxin Lianziyin is not particularly limited, but usually, Rehmanniae Radix 2 to 5 parts by weight, preferably 3 to 4 parts by weight; Scutellariae Radix 1.5 to 4 parts by weight, preferably 2 to 3 parts by weight; Plantaginis Semen 2 to 4 parts by weight, preferably 2.5 to 3.5 parts by weight; Radix Glehniae 1.5 to 5 parts by weight, preferably 2 to 4 parts by weight; Schisandrae Chinensis Fructus 1.5 to 3 parts by weight, preferably 2 to 2.5 parts by weight; Coptidis Rhizoma 1.5 to 3 parts by weight, preferably 2 to 2.5 parts by weight; Glycyrrhizae Radix 1 to 4 parts by weight, preferably 2 to 3 parts by weight; Gardeniae Fructus 1 to 3 parts by weight, preferably 2 to 2.5 parts by weight; and Paeoniae Radix Alba 0.7 to 2 parts by weight, preferably 0.7 to 1 part by weight can be mentioned.

[0014] As a preferable example of the crude drug preparation used for the production of the Qingxin Lianzi Yin extract used in the present invention, there may be mentioned 3.5 parts by weight of Renni (Nelumbo nucifera Gaertn. seed), 2.1 parts by weight of Bakumondou (Paeonia lactiflora Pall. root bark), 2.8 parts by weight of Bukuryou (Scutellaria baicalensis Georgi root), 3.5 parts by weight of Ninjin (Daucus carota L. var. sativus Hoffm. root), 2.1 parts by weight of Shazenshi (Gardenia jasminoides Ellis fruit), 2.1 parts by weight of Ougon (Curcuma longa L. rhizome), 2.8 parts by weight of Ougi (Alpinia officinarum Hance rhizome), 2.1 parts by weight of Jikoppi (Gentiana scabra Bunge root), and 0.7 parts by weight of Kanzou (Glycyrrhiza glabra L. root).

[0015] The Qingxin Lianzi Yin extract used in the present invention contains glycyrrhizic acid in an amount of 0.22% by weight or more. Such a traditional Chinese medicine preparation containing the Qingxin Lianzi Yin extract with a high content of glycyrrhizic acid exhibits a peculiar unpleasant sweetness. However, in the traditional Chinese medicine preparation of the present invention, although the content of glycyrrhizic acid is high, the peculiar unpleasant sweetness is suppressed.

[0016] Since the traditional Chinese medicine preparation of the present invention is excellent in the effect of suppressing the peculiar unpleasant sweetness, even when the content of glycyrrhizic acid is even higher, it can effectively suppress the unpleasant sweetness. From this perspective, preferable examples of the glycyrrhizic acid contained in the Qingxin Lianzi Yin extract used in the present invention include 0.3% by weight or more, more preferably 0.4% by weight or more, still more preferably 0.45% by weight or more, and even more preferably 0.48% by weight or more. The upper limit of the glycyrrhizic acid contained in the Qingxin Lianzi Yin extract used in the present invention is not particularly limited, but for example, it may be 1% by weight or less, preferably 0.9% by weight or less.

[0017] In addition, the Qingxin Lianzi Yin extract used in the present invention contains ononin in an amount of 8 parts by weight or more per 100 parts by weight of glycyrrhizic acid. Thereby, the peculiar unpleasant sweetness of the high content of glycyrrhizic acid is suppressed. From the perspective of further enhancing the effect of suppressing the unpleasant sweetness, preferable examples of the content of ononin per 100 parts by weight of glycyrrhizic acid include 8.3 parts by weight or more or 9 parts by weight or more, more preferably 11 parts by weight or more, still more preferably 13 parts by weight or more, even more preferably 15 parts by weight or more, and particularly preferably 16 parts by weight or more. The upper limit of the content of ononin per 100 parts by weight of glycyrrhizic acid is not particularly limited, but for example, it may be 23 parts by weight or less, preferably 19 parts by weight or less.

[0018] As for the content of ononin in the Qingxin Lianziyin extract used in the present invention, the amount relative to 100 parts by weight of glycyrrhizic acid is not particularly limited as long as it is the above-mentioned amount. For example, it is 0.02% by weight or more, preferably 0.028% by weight or more, more preferably 0.05% by weight or more, still more preferably 0.07% by weight or more, and particularly preferably 0.08% by weight or more. The upper limit of the ononin content contained in the Qingxin Lianziyin extract used in the present invention is not particularly limited, but for example, it is 0.4% by weight or less, preferably 0.2% by weight or less.

[0019] The glycyrrhizic acid contained in the Qingxin Lianziyin extract used in the present invention is at least partially derived from Glycyrrhiza glabra that constitutes the Qingxin Lianziyin, but preferably all of it is derived from Glycyrrhiza glabra that constitutes the Qingxin Lianziyin.

[0020] The ononin contained in the Qingxin Lianziyin extract used in the present invention is at least partially derived from Pueraria lobata that constitutes the Qingxin Lianziyin, but preferably all of it is derived from Pueraria lobata that constitutes the Qingxin Lianziyin.

[0021] As an example of a method for adjusting the content of glycyrrhizic acid and the content ratio of ononin to it in the Qingxin Lianziyin extract within the above ranges, there is a method of adding the lacking glycyrrhizic acid and / or ononin after adjusting the Qingxin Lianziyin extract. In this method, as the components used for adding glycyrrhizic acid and / or ononin, they may be those purified compounds, or they may be Glycyrrhiza glabra extract, Pueraria lobata extract, or an extract of a mixed crude drug of Glycyrrhiza glabra and Pueraria lobata.

[0022] From the perspective of further enhancing the inhibitory effect on unpleasant sweetness, as another example of the method for adjusting the content of glycyrrhizic acid and the content ratio of ononin thereto in the extract of Qingxin Lianzi Decoction within the above ranges, it is preferable to use, for at least a part of the licorice or the shredded products of licorice and licorice root used in the crude drug mixture constituting Qingxin Lianzi Decoction, finely shredded products crushed to a size passing through a sieve with a nominal mesh size of 1 mm. In this method, when using the above-mentioned finely shredded products for a part of the licorice or the shredded products of licorice and licorice root used in the crude drug mixture constituting Qingxin Lianzi Decoction (that is, when a part of the shredded products is the above-mentioned finely shredded products and the rest are the normally sized shredded products crushed to a size not passing through a sieve with a nominal mesh size of 4 mm), the ratio of the above-mentioned finely shredded products of licorice to 1 part by weight of the total shredded products of licorice, and the ratio of the above-mentioned finely shredded products of licorice root to 1 part by weight of the total shredded products of licorice root, in either case, are both 0.3 parts by weight or more. From the perspective of further enhancing the inhibitory effect on unpleasant sweetness, in either case, the ratio is preferably 0.5 parts by weight or more, more preferably 0.7 parts by weight or more, and even more preferably 0.9 parts by weight or more. From the perspective of further enhancing the inhibitory effect on unpleasant sweetness, it is most preferable that all of the licorice or the shredded products of licorice and licorice root used in the crude drug mixture constituting Qingxin Lianzi Decoction are the above-mentioned finely shredded products.

[0023] The extract of Qingxin Lianzi Decoction can be obtained by subjecting the above-mentioned mixed crude drugs to an extraction treatment and, if necessary, concentrating or further drying the obtained extract.

[0024] The extraction solvent used for the extraction treatment of the extract of Qingxin Lianzi Decoction is not particularly limited, and examples include water or aqueous ethanol, preferably water. Also, the liquid extract of Qingxin Lianzi Decoction can be obtained by concentrating the obtained extract. Furthermore, the dried extract powder of Qingxin Lianzi Decoction can be obtained by drying the liquid extract. The method of the drying treatment is not particularly limited, and examples include the spray drying method and the method of adding a suitable adsorbent (such as anhydrous silicic acid, starch, etc.) to the soft extract with an increased extract concentration to obtain an adsorbed powder.

[0025] The extract form of Qingxin Lianzi Yin may be any of liquid extracts such as flowing extracts and soft extracts, or solid dried extract powders.

[0026] The content of Qingxin Lianzi Yin extract in the traditional Chinese medicine preparation of the present invention is not particularly limited as long as the effects of the present invention can be achieved. However, in terms of the amount of dried extract powder of Qingxin Lianzi Yin, it is 10 to 100% by weight, preferably 20 to 90% by weight, more preferably 40 to 80% by weight, and still more preferably 60 to 70% by weight. In the present invention, the conversion in terms of the amount of dried extract powder of Qingxin Lianzi Yin means that when using the dried extract powder of Qingxin Lianzi Yin, it is the amount itself, and when using the liquid extract of Qingxin Lianzi Yin, it is the amount converted to the remaining amount after removing the solvent. Further, when the dried extract powder of Qingxin Lianzi Yin contains additives such as adsorbents added during production, it is the amount excluding the additives.

[0027] Other ingredients The traditional Chinese medicine preparation of the present invention may consist of Qingxin Lianzi Yin extract alone, or may contain additives and bases according to the preparation form. Such additives and bases are not particularly limited as long as they are pharmaceutically acceptable. For example, excipients, binders, disintegrants, lubricants, isotonic agents, plasticizers, dispersants, emulsifiers, solubilizers, wetting agents, stabilizers, suspending agents, adhesives, coating agents, brightening agents, water, oils and fats, waxes, hydrocarbons, fatty acids, higher alcohols, esters, water-soluble polymers, surfactants, metal soaps, lower alcohols, polyhydric alcohols, pH adjusters, buffers, antioxidants, ultraviolet inhibitors, preservatives, flavoring agents, fragrances, powders, thickeners, pigments, chelating agents, etc. These additives may be used alone or in combination of two or more. Further, the content of these additives and bases is appropriately set according to the types of additives and bases used, the preparation form of the traditional Chinese medicine preparation, and the like.

[0028] In addition to the Qingxin Lianziyin extract, the traditional Chinese medicine preparation of the present invention may also contain other nutritional components and pharmacological components as needed. Such nutritional components and pharmacological components are not particularly limited as long as they are pharmaceutically acceptable. For example, antacids, stomachic agents, digestive agents, intestinal regulators, antispasmodics, mucosal repair agents, anti-inflammatory agents, astringents, antiemetics, antitussives, expectorants, anti-inflammatory enzyme agents, sedative hypnotics, antihistamines, caffeine, cardiotonic diuretics, antibacterial agents, vasoconstrictors, vasodilators, local anesthetics, crude drug extracts, vitamins, menthols, etc. These nutritional components and pharmacological components may be used alone or in combination of two or more. In addition, the content of these components is appropriately set according to the type of components used, etc.

[0029] Form of preparation The dosage form of the traditional Chinese medicine preparation of the present invention is not particularly limited as long as it can be administered orally. For example, solid preparations such as powders, fine granules, granules, tablets (preferably plain tablets), lozenges, chewable tablets, capsules (soft capsules, hard capsules), pills, etc.; semi-solid preparations such as jelly; liquid preparations such as solutions, suspensions, syrups, etc. are mentioned. Preferably, powders, fine granules, granules, lozenges, chewable tablets are mentioned.

[0030] Manufacturing method The manufacturing method of the traditional Chinese medicine preparation of the present invention may be formulated according to the usual formulation techniques employed in the pharmaceutical field using the Qingxin Lianziyin extract obtained by the above method and containing specific components in specific ratios, and using other components as needed.

[0031] 2. Method for suppressing sweetness As described above, in the extract of Qingxin Lianzi Decoction with a high content of glycyrrhizic acid, by adjusting to contain a specific amount of ononin with respect to glycyrrhizic acid, the effect of suppressing the unpleasant sweet taste peculiar to the high content of glycyrrhizic acid is expressed. Therefore, the present invention further provides a method for suppressing the sweet taste of glycyrrhizic acid in a traditional Chinese medicine preparation containing an extract of Qingxin Lianzi Decoction containing 0.22% by weight or more of glycyrrhizic acid, wherein in the extract of Qingxin Lianzi Decoction, the content of ononin is adjusted to be 8 parts by weight or more with respect to 100 parts by weight of glycyrrhizic acid.

[0032] In the method for suppressing the sweet taste of the present invention, regarding the content and ratio of the components contained in the extract of Qingxin Lianzi Decoction used, the method for adjusting the content and ratio, the types and contents of the components formulated in the traditional Chinese medicine preparation, and the preparation form, etc., they are as described in the above-mentioned "1. Traditional Chinese Medicine Preparation".

Examples

[0033] Hereinafter, the present invention will be specifically described by way of examples, but the present invention is not limited to these examples.

[0034] (1) Preparation of the extract of Qingxin Lianzi Decoction As raw medicinal herbs, 3.5 parts by weight of Rehmanniae Radix, 2.1 parts by weight of Scutellariae Radix, 2.8 parts by weight of Poria, 3.5 parts by weight of Radix Glehniae, 2.1 parts by weight of Platycodonis Radix, 2.1 parts by weight of Polygoni Multiflori Caulis, 2.8 parts by weight of Anemarrhenae Rhizoma, 2.1 parts by weight of Gardeniae Fructus, and 0.7 part by weight of Glycyrrhizae Radix were used. Among these, for Glycyrrhizae Radix and Anemarrhenae Rhizoma, ordinary shredded materials (shredded materials with a size not passing through a sieve with a nominal mesh size of 4 mm), fine shredded materials (shredded materials with a size passing through a sieve with a nominal mesh size of 1 mm), or a mixture of 0.5 part by weight of ordinary shredded materials and 0.5 part by weight of fine shredded materials were used. The sizes of the shredded materials of Glycyrrhizae Radix and Anemarrhenae Rhizoma were made uniform. For the other medicinal herbs, ordinary shredded materials (shredded materials with a size not passing through a sieve with a nominal mesh size of 4 mm) were used.

[0035] These chopped crude drugs were mixed, extracted at about 100 °C for 1 hour using 10 times the weight of water, and centrifuged to obtain an extract. The extract was concentrated under reduced pressure and dried using a spray dryer to obtain the extract powder of Qingxin Lianzi Yin. The obtained extract powder of Qingxin Lianzi Yin was 2238 mg per 21.7 g of the raw material crude drug mixture. In addition, the drying by the spray dryer was carried out by dropping the extract onto an atomizer rotating at 10,000 rpm and supplying hot air of 150 °C air.

[0036] (2) Measurement of glycyrrhizic acid and ononin The contents of glycyrrhizic acid and ononin contained in the obtained extract of Qingxin Lianzi Yin were measured by the following method. In the following, the description of "(x→y)" regarding the dilution ratio of the diluted reagent means that x volume parts of the reagent were diluted to y volume parts with water.

[0037] (Measurement of glycyrrhizic acid) Precisely weigh about 0.2 g of the dried extract, add 20 mL of ethyl acetate and 10 mL of water, and shake for 10 minutes. This was centrifuged, and after removing the upper layer, 20 mL of ethyl acetate was added and the same operation was performed to remove the upper layer. 10 mL of methanol was added to the obtained aqueous layer, shaken for 30 minutes, then centrifuged, and the supernatant was collected. 20 mL of diluted methanol (1→2) was added to the residue, shaken for 5 minutes, then centrifuged, and the supernatant was collected and combined with the previous supernatant, and diluted methanol (1→2) was added to make exactly 50 mL to obtain a sample solution. Separately, precisely weigh about 10 mg of a glycyrrhizic acid standard product, dissolve it in diluted methanol (1→2) to make exactly 100 mL to obtain a standard solution.

[0038] Precisely take 10 μL each of the sample solution and the standard solution, perform a test by liquid chromatography under the following conditions, and measure the peak area AT of glycyrrhizic acid in the sample solution and the peak area AS of the standard solution of each liquid.

[0039] (Liquid chromatography test conditions) Detector: Ultraviolet absorptiometer (measurement wavelength: 254 nm) Column: A stainless steel tube with an inner diameter of 4.6 mm and a length of 15 cm was filled with 5-μm octadecylsilylated silica gel for liquid chromatography. Column temperature: A constant temperature around 40 °C Mobile phase: 3.85 g of ammonium acetate was dissolved in 720 mL of water, and 5 mL of acetic acid (100) and 280 mL of acetonitrile were added. Flow rate: 1.0 mL per minute

[0040] Using the obtained peak areas AT and AS, the amount (mg) of glycyrrhizic acid per weighed amount was derived based on the following formula.

[0041]

Equation

[0042] <Measurement of ononin> Approximately 0.5 g of the dried extract was precisely weighed, 50 mL of methanol was accurately added, and the mixture was shaken for 30 minutes and then ultrasonically extracted for 30 minutes. This solution was filtered, and the filtrate was used as the sample solution. Separately, an ononin standard was precisely weighed, methanol was added and dissolved to make exactly 100 mL. This methanol solution was irradiated with ultrasonic waves to obtain the standard solution. Exactly 10 μL each of the sample solution and the standard solution were taken, and a test was performed by liquid chromatography under the following conditions to measure the peak area AT of ononin in the sample solution and the peak area AS of the standard solution.

[0043] (Liquid chromatography test conditions) Detector: Ultraviolet absorptiometer (measurement wavelength: 249 nm) Column: A stainless steel tube with an inner diameter of 4.6 mm and a length of 15 cm was filled with 5-μm octadecylsilylated silica gel for liquid chromatography. Column temperature: A constant temperature around 40 °C Mobile phase: Diluted acetic acid (1→500) / acetonitrile (4 / 1 (volume ratio)) mixture Flow rate: 1.0 mL per minute

[0044] Using the obtained peak areas AT and AS, the amount of ononin was derived based on the following formula.

[0045]

Number

[0046] (3) Judgment of sweetness score Ten monitors who had received training on taste placed 1 g of the obtained Qingxin Lianziyin extract directly on their tongues and evaluated the perceived sweetness on a 5-point scale (1 being weak and 5 being strong) using VAS. The scores of all the monitors were averaged. The obtained average score was classified according to the following criteria to obtain a sweetness score. The results are shown in Table 1.

[0047] <Sweetness score> +++++: 4.7 or more ++++: 3.7 or more and less than 4.7 +++: 2.7 or more and less than 3.7 ++: 1.7 or more and less than 2.7 +: Less than 1.7

[0048]

Table 1

[0049] As shown in Table 1, when prepared using ordinary shredded ingredients, Qingxin Lianziyin extracts containing 0.18% by weight and 0.23% by weight of glycyrrhizic acid (Reference Example 1, Comparative Example 1) were obtained. However, the Qingxin Lianziyin extract containing 0.23% by weight of glycyrrhizic acid (Comparative Example 1) exhibited an extremely strong sweetness. According to the monitors, this extremely strong sweetness was an unpleasant sweetness peculiar to glycyrrhizic acid.

[0050] On the one hand, when half of the weight of the shredded licorice and mugwort was replaced with finely shredded materials for preparation, an extract of Qingxin Lianzi Decoction (Examples 1 and 2) with increased glycyrrhizic acid and an increased ratio of ononin to glycyrrhizic acid was obtained. And in these extracts of Qingxin Lianzi Decoction (Examples 1 and 2), although the content of glycyrrhizic acid, which causes an unpleasant strong sweetness, was high, the sweetness was significantly reduced.

[0051] Furthermore, when all finely shredded materials were used as the shredded licorice and mugwort for preparation, an extract of Qingxin Lianzi Decoction (Example 3) with further increased glycyrrhizic acid and a further increased ratio of ononin to glycyrrhizic acid was obtained. And in this extract of Qingxin Lianzi Decoction (Example 3), although the content of glycyrrhizic acid, which causes an unpleasant strong sweetness, was even higher, the sweetness was even more significantly reduced.

Claims

1. A Kampo preparation containing Coptidis Decoction with Lotus Seeds extract, wherein in the Coptidis Decoction with Lotus Seeds extract, the content of glycyrrhizic acid is 0.22 to 1% by weight, and the content of ononin relative to 100 parts by weight of glycyrrhizic acid is 8.34 to 23 parts by weight.

2. The Kampo preparation according to claim 1, which is a powder, fine granules, granules, troche, or chewable agent.

3. A method for suppressing the sweetness of glycyrrhizic acid in a Kampo preparation containing Coptidis Decoction with Lotus Seeds extract containing 0.22 to 1% by weight of glycyrrhizic acid, wherein in the Coptidis Decoction with Lotus Seeds extract, the content of ononin is adjusted to be 8.34 to 23 parts by weight relative to 100 parts by weight of glycyrrhizic acid.

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