Method for domesticating marine shrimp and method for cultivating by aquaponics

Abstract

Provided are a method for domesticating marine shrimp and a method for growing marine shrimp by aquaponics, which can surely improve the efficiency and promote the growth of land-based farming of marine shrimp. 【Solution means】 The method for domesticating marine shrimp A in the present invention to a low-salt environment has a salt concentration reduction step of reducing the salt concentration of the breeding water 111 in which marine shrimp A is bred by 0.01 to 0.09% by mass per day. The method for growing by aquaponics includes a salt concentration reduction step of reducing the salt concentration of the breeding water in which marine shrimp is bred by 0.01 to 0.09% by mass per day, a decomposition liquid generation step of biodegrading the excrement of the marine shrimp to generate a decomposition liquid, and a decomposition liquid supply step of supplying the decomposition liquid generated in the decomposition liquid generation step to the plants hydroponically cultivated using the breeding water whose salt concentration has been reduced by the salt concentration reduction step.

Description

Technical Field

[0001] The present invention relates to a method for acclimating marine shrimp to low-salinity breeding water having a salinity concentration lower than that of seawater, and a cultivation method by aquaponics for performing hydroponics using the low-salinity breeding water.

Background Art

[0002] Conventionally, methods for terrestrial cultivation of crustaceans have been studied. Marine shrimp are known for their very rapid growth, taking about 3 to 4 months from juvenile shrimp with a body length of 0.5 cm to edible shrimp with a body length of 12 to 15 cm. Among them, Litopenaeus vannamei is attracting attention as it has high environmental adaptability, disease resistance, and growth rate, can grow on low-protein feed, has a high protein content after growth, and can survive for a long time outside water, making it easy to achieve high profitability. However, in Japan, as a method for cultivating marine shrimp, closed-loop circulation or flow-through terrestrial cultivation is carried out using seawater or breeding water having a salinity concentration equivalent to seawater. Therefore, the feasible area is limited to areas near the sea, and further costs are incurred for proper water quality management. For this reason, there is a strong desire for a method for cultivating shrimp that can be cultivated in an environment closer to fresh water or a completely fresh water environment with a lower salinity concentration.

[0003] Patent Document 1 discloses a method for cultivating edible marine shrimp using breeding water having a salinity concentration of 1 to 10 ppt (1 to 10‰ = 0.1 to 1.0%). Patent Document 2 describes adjusting breeding water having a salinity concentration at which marine organisms can survive to reduce it by 0.10 to 0.50% by mass per day to obtain breeding water having a salinity concentration of about 0.007 to 0.500% by mass.

Prior Art Documents

Patent Documents

[0004]

Patent Document 1

Patent Document 2

Summary of the Invention

Problems to be Solved by the Invention

[0005] According to the method disclosed in Patent Document 1, by gradually replacing the seawater breeding water, the shrimp are gradually adapted to a low salinity concentration. When the final salinity concentration is 0.5% (5 ppt), it is replaced in one day, and when it is 0.1% (1 ppt), the whole amount is replaced over 3 to 7 days (see paragraphs

[0027] -

[0028] of the specification). Also, regarding the relationship between the salinity concentration and the growth rate of shrimp, the group reared in seawater with a salinity of 3% (30 ppt) and the shrimp reared in breeding water with a salinity of 0.5% (5 ppt) have a higher weight gain rate, body growth rate, and survival rate compared to the two groups reared at low salinity concentrations of 0.15% (1.5 ppt) and 0.2% (2 ppt) (see paragraph

[0057] of the specification, Figure 2).

[0006] Here, in the adaptation of shrimp to a low salinity concentration, the survival rate of shrimp depends on the amount of salinity reduction per day, but this point is not disclosed in Patent Document 1, so it is not possible to increase the survival rate of shrimp and improve the efficiency of land-based aquaculture. Also, according to the method disclosed in Patent Document 1, in a low salinity environment including a completely fresh water environment (salinity concentration of 0.05% or less) and an environment close to fresh water (salinity concentration of less than 0.5%), there is a problem that it is not possible to surely improve the efficiency and promote the growth of marine shrimp compared to the seawater environment.

[0007] Also, according to the method disclosed in Patent Document 2, for breeding water with a salinity concentration of 1.0% by mass, when the salinity concentration is reduced by 0.10% by mass or more per day, the survival rate of shrimp may decrease. Therefore, there is a concern that it is not possible to surely promote the growth of marine shrimp.

[0008] Therefore, the present invention has been devised in view of the above problems, and its object is to provide a method for acclimating marine shrimp and a method for growing them by aquaponics that can surely improve the efficiency and promote the growth of land-based aquaculture of marine shrimp.

Means for Solving the Problem

[0009] The method for acclimating marine shrimp in the first invention is characterized by having a salinity concentration reduction step of reducing the salinity concentration of the breeding water in which the marine shrimp are bred by in the range of 0.20 mass% or less 0.01% by mass to 0.09% by mass per day.

[0010] The cultivation method by aquaponics in the second invention is characterized by having a salinity concentration reduction step of reducing the salinity concentration of the breeding water in which the marine shrimp are bred by in the range of 0.20 mass% or less 0.01% by mass to 0.09% by mass per day, a decomposition liquid generation step of biodegrading the excrement of the marine shrimp to generate a decomposition liquid, and a decomposition liquid supply step of supplying the decomposition liquid generated in the decomposition liquid generation step to plants hydroponically cultivated using the breeding water whose salinity concentration has been reduced by the salinity concentration reduction step.

Effect of the Invention

[0011] According to the first invention, it has a salinity concentration reduction step of reducing the salinity concentration of the breeding water in which the marine shrimp are bred by 0.01% by mass to 0.09% by mass per day. Therefore, it is possible to efficiently acclimate the marine shrimp to a low-salinity environment while suppressing a decrease in the survival rate of the marine shrimp. Thereby, it is possible to surely improve the efficiency and promote the growth of land-based aquaculture of marine shrimp.

[0012] According to the second invention, it has a salinity concentration reduction step of reducing the salinity concentration of the breeding water in which the marine shrimp are bred by 0.01% by mass to 0.09% by mass per day, a decomposition liquid generation step of biodegrading the excrement of the shrimp to generate a decomposition liquid, and a decomposition liquid supply step of supplying the generated decomposition liquid to plants hydroponically cultivated using the breeding water whose salinity concentration has been reduced. Therefore, it is possible to efficiently acclimate the marine shrimp to a low-salinity environment while suppressing a decrease in the survival rate of the marine shrimp, and further to efficiently increase the weight of the plants. Thereby, it is possible to improve the efficiency of land-based aquaculture of marine shrimp and more surely promote growth, while also improving the efficiency of plant cultivation.

Brief Description of the Drawings

[0013]

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Embodiments for Carrying Out the Invention

[0014] Hereinafter, with reference to the drawings, an example of a method for domesticating marine shrimp A, a cultivation method by aquaponics 2, a domestication system 1 for marine shrimp A, and an aquaponics 2 in the embodiments of the present invention will be described in detail. Note that the configurations in each figure are schematically described for the purpose of explanation, and for example, the size of each configuration and the comparison of sizes for each configuration may be different from those in the figure.

[0015] (Method for Domesticating Marine Shrimp A) With reference to FIGS. 1 to 3, an example of the method for domesticating marine shrimp A in the present embodiment will be described. First, the domestication system 1 used in the method for domesticating marine shrimp A in the present embodiment will be described.

[0016] <Domestication System 1> The acclimation system 1 is a device for cultivating marine shrimp A. The acclimation system 1 includes, for example, as shown in FIG. 1, a housing part 11 and a salinity reduction part 12. The acclimation system 1 may further include a filtration device 51, a disinfection device 52, a bubble generation device 53, a water quality monitoring device 54, a temperature control device 55, an automatic feeding device 56, a lighting part 57, and artificial waterweeds 6.

[0017] The acclimation system 1 is used in areas where it is difficult to obtain seawater, such as onshore aquaculture. The acclimation system 1 may be, for example, a flow-through type that continuously draws in seawater or water with a salinity lower than that of seawater into the housing part 11, or a closed-loop type that recirculates the water in the housing part 11.

[0018] <Housing part 11> The housing part 11 houses the breeding water 110 and the marine shrimp A. The housing part 11 is, for example, a cage or an aquarium that houses the marine shrimp A.

[0019] The housing part 11 is connected, for example, to a circulation pipe 31 for recycling the drained water. The breeding water 110 is circulated through the housing part 11 via the circulation pipe 31, for example. As the circulation pipe 31, a known water supply pipe such as a polyethylene pipe may be used, for example.

[0020] The housing part 11 is connected, for example, to a water supply pipe 32 for supplying water into the housing part 11. The housing part 11 may be supplied with water from the salinity reduction part 12 via the water supply pipe 32, for example. As the water supply pipe 32, a water supply pipe of the same type as the circulation pipe 31 may be used, for example.

[0021] The housing part 11 is connected, for example, to a drainage pipe 33 for discharging the water in the housing part 11. The housing part 11 may drain water to the salinity reduction part 12 via the drainage pipe 33, for example. As the drainage pipe 33, a water supply pipe of the same type as the circulation pipe 31 may be used, for example.

[0022] The housing part 11 is supplied with water filtered through the filtration device 51, for example. The housing part 11 is supplied with water disinfected through the disinfection device 52, for example.

[0023] <Rearing water 110> The rearing water 110 is the rearing water in which the reduction of the salt concentration has been completed by the salt concentration reduction unit 12. The rearing water 110 has, for example, a salt concentration of about 0.007 to 0.500% by mass, which is lower than the salt concentration of seawater (about 3.400% by mass). In the example of FIG. 1, the state after the marine shrimp A, which cannot originally survive at the salt concentration of the rearing water 110, has been acclimated to the salt concentration of the rearing water 110 is shown. This is because the salt concentration reduction unit 12 gradually reduces the salt concentration of the rearing water (rearing water 111 described later) at a salt concentration at which marine organisms can survive under predetermined conditions while the marine shrimp A is being reared, resulting in the rearing water 110 having a salt concentration of about 0.007 to 0.500% by mass. Details of the method for acclimating the marine shrimp A to the rearing water 110 will be described later.

[0024] The rearing water 110 contains, as main components, for example, Mg 2+ (magnesium ion), Ca 2+ (calcium ion), K + (potassium ion), SO4 2- (sulfate ion).

[0025] The rearing water 110 contains, for example, as the mass of the solute per liter of the rearing water, Mg 2+ of less than 35 mg or more than 320 mg, Ca 2+ of less than 25 mg or more than 250 mg, K + of less than 8 mg or more than 80 mg. If any of these conditions is met, the survival rate of the marine shrimp A will be less than 60% due to causes such as incomplete molting, and thus the production efficiency of the marine shrimp A cannot be reliably improved. Therefore, the rearing water 110 preferably contains 35 to 320 mg of Mg 2+ as the mass of the solute per liter of the rearing water, 25 to 250 mg of Ca 2+ and 8 to 80 mg of K + .

[0026] Also, the ratio of the mass of Mg + to the mass of K 2+ is defined as the first mass ratio, and the ratio of the mass of K +Ca per mass of 2+ The mass ratio of Ca is the second mass ratio. 2+ of Mg 2+ When the mass ratio of the above is defined as the third mass ratio, if the first mass ratio is less than 0.7 or more than 5.0, the second mass ratio is less than 0.5 or more than 5.0, or the third mass ratio is less than 0.7 or more than 1.4, the survival rate of the saltwater shrimp A will be less than 60% due to incomplete molting, etc., and it will be difficult to reliably improve the production efficiency of the saltwater shrimp A. For this reason, it is preferable that the first mass ratio is 0.7 to 5.0, the second mass ratio is 0.5 to 5.0, and the third mass ratio is 0.7 to 1.4.

[0027] That is, the breeding water 110 has a solute mass of 35 to 320 mg of Mg per 1 L of breeding water. 2+ and 25-250 mg of Ca. 2+ and 8 to 80 mg of K. + and a first mass ratio (Mg 2+ mass / K + The second mass ratio (Ca 2+ mass / K + The third mass ratio (Mg 2+ mass / Ca 2+ It is preferable that the mass of the shrimp A is 0.7 to 1.4. In this case, the survival rate of the saltwater shrimp A becomes 75% or more, and the survival rate of the saltwater shrimp A can be improved. This can improve the efficiency of land culture of the saltwater shrimp A. The relationship between the breeding water 110 and the survival rate of the saltwater shrimp A will be described in detail later.

[0028] Moreover, the third mass ratio of the breeding water 110 is preferably 0.7 to 1.3. In this case, the survival rate of the saltwater shrimp A becomes 78% or more, which can further improve the survival rate of the saltwater shrimp A and further improve the efficiency of land-based culture of the saltwater shrimp A.

[0029] In addition, the breeding water 110 preferably satisfies the combination of the first mass ratio of 0.7 to 4.0 and the second mass ratio of 0.5 to 4.0, more preferably satisfies the combination of the first mass ratio of 1.0 to 4.0 and the second mass ratio of 1.0 to 3.0, and even more preferably satisfies the combination of the first mass ratio of 1.5 to 4.0 and the second mass ratio of 2.0 to 3.0. In this case, the survival rate of the seawater shrimp A is 82% or more, the survival rate of the seawater shrimp A can be further improved, and further improvement in the efficiency of land-based aquaculture for the seawater shrimp A can be achieved.

[0030] When the breeding water 110 does not contain any SO4 2- compared with the case of containing SO4 2- problems occur in the molting of the seawater shrimp A and the mortality rate increases, so it is preferable to contain SO4 2- In addition, the breeding water 110 preferably contains 70 to 400 mg of SO4 2- as the mass of the solute per liter of the breeding water.

[0031] Generally, regarding the environment of the breeding water 110 during shrimp aquaculture, as the mass of the solute per liter of the breeding water, CaCl2 is about 80 to 100 mg (the same function as Ca 2+ ), MgSO4 is about 250 to 300 mg (the same function as Mg 2+ ), K2SO4 is about 100 mg (the same function as K + ), and it is desirable to maintain an environment with a total alkalinity of 120 mg / L and a pH of about 7.8. In addition, SO4 2- is contained in general seawater to some extent, is involved in the osmoregulation (regulation of salt concentration inside and outside the body fluid) of the body fluids of fish and crustaceans, and may play an important role in life processes such as molting, and is a part of important environmental conditions for organisms such as fish and crustaceans. Therefore, it is also preferable that the breeding water 110 contains SO4 2- as an important factor affecting the normal growth and survival of the seawater shrimp A.

[0032] Regarding the mass (concentration) of each of the above ions, they are prepared by adding the corresponding salts. Specifically, Mg 2+When the breeding water 110 contains 40 mg / L, it can be adjusted by adding about 198.30 mg / L of magnesium sulfate. Also, for Mg 2+ When the breeding water 110 contains 240 mg / L, it can be adjusted by adding about 1,189.80 mg / L of magnesium sulfate.

[0033] Also, for Ca 2+ When the breeding water 110 contains 30 mg / L, it can be adjusted by adding about 83.08 mg / L of calcium chloride. Also, for Ca 2+ When the breeding water 110 contains 200 mg / L, it can be adjusted by adding about 553.86 mg / L of calcium chloride.

[0034] Also, for K + When the breeding water 110 contains 10 mg / L, it can be adjusted by adding about 22.28 mg / L of potassium sulfate. Also, for K + When the breeding water 110 contains 60 mg / L, it can be adjusted by adding about 133.68 mg / L of potassium sulfate.

[0035] Note that the above addition amounts of salts are theoretical values calculated assuming a water temperature of 28°C and a pH of 7.5. However, in reality, the ions in the breeding water 110 may not completely ionize, so the actual addition amount of salts required may increase or decrease compared to the theoretical value. Also, note that the required addition amount of salts can increase or decrease due to various factors including the solubility of each salt, the pH of the water, the temperature of the water, and the influence of other dissolved substances.

[0036] The breeding water 110 has its water quality parameters necessary for the cultivation of the seawater shrimp A monitored, for example, via the water quality monitoring device 54. The breeding water 110 is controlled to a water temperature at which the seawater shrimp A can survive, for example, via the temperature control device 55.

[0037] <Salt concentration reduction unit 12> The salt concentration reduction unit 12 reduces the salt concentration of the breeding water 110 accommodated together with the shrimps in the accommodation unit 11. The salt concentration reduction unit 12 may continuously or intermittently reduce the salt concentration of the breeding water 110 in the accommodation unit 11.

[0038] The salinity concentration reduction unit 12 reduces the salinity concentration of the breeding water 111 by 0.01% by mass to 0.09% by mass per day. When the salinity concentration of the breeding water 111 is extremely reduced by 0.09% by mass per day, the survival rate of the marine shrimp A may be 0%, so the reliable efficiency improvement and growth promotion of land-based aquaculture for the marine shrimp A cannot be achieved. Also, when the salinity concentration of the breeding water 111 is reduced by less than 0.01% by mass per day, the period for acclimating the marine shrimp A to a low salinity concentration becomes excessively long, and the amount of work such as water replacement increases, so the reliable efficiency improvement and growth promotion of land-based aquaculture for the marine shrimp A cannot be achieved.

[0039] Therefore, it is preferable that the salinity concentration reduction unit 12 reduces the salinity concentration of the breeding water 111 by 0.01% by mass to 0.09% by mass per day. In this case, it is possible to efficiently acclimate to a low salinity environment while suppressing a decrease in the survival rate of the marine shrimp A. Thereby, reliable efficiency improvement and growth promotion of land-based aquaculture for the marine shrimp A can be achieved. Note that the utility of the present invention regarding the acclimation efficiency of the marine shrimp A will be described in the examples below.

[0040] The salinity concentration reduction unit 12 has, for example, a water supply tank 13 and a waste water tank 14.

[0041] The water supply tank 13 stores dilution water 130 having a salinity concentration lower than the salinity concentration at which marine organisms can survive. The water supply tank 13 supplies the dilution water 130 to the storage unit 11 via, for example, a water supply pipe 32. As the dilution water 130, for example, groundwater or dechlorinated tap water may be used.

[0042] The waste water tank 14 stores, as waste water 140, a part of the breeding water 110 discharged from the storage unit 11. The waste water tank 14 discharges the waste water 140 from the storage unit 11 via, for example, a drain pipe 33.

[0043] <Marine shrimp A> Marine shrimp A refers to shrimp that belong to marine organisms and does not include shrimp that belong to freshwater organisms. Marine shrimp A is, for example, edible shrimp. In order to efficiently acclimatize marine shrimp A to the breeding water 110 with a lower salt concentration than seawater, particularly juvenile shrimp are used. Specific examples of marine shrimp A include Penaeidae (Kuruma shrimp, vannamei shrimp, banana shrimp, etc.), Sergestidae (sakura shrimp, etc.), Palinuridae (spiny lobster, button shrimp, Hokkaido shrimp, etc.), Sergestidae (Japanese common prawn, etc.), and the like.

[0044] For marine shrimp A, for example, food is automatically supplied via the automatic feeding device 56.

[0045] <Filter device 51> The filter device 51 filters the water supplied to the accommodation part 11. The filter device 51 may be provided outside the accommodation part 11 or may be provided inside the accommodation part 11.

[0046] The filter device 51 filters, for example, the circulating water supplied to the accommodation part 11 via the circulation pipe 31 or the dilution water 130 supplied to the accommodation part 11 via the water supply pipe 32. At this time, the filter device 51 may be provided in the circulation pipe 31 or in the water supply pipe 32.

[0047] The filter device 51 has, for example, a known filter medium. Specifically, it has a sponge for physical filtration, a porous filter medium with bacteria for biological filtration, activated carbon for chemical filtration, and the like.

[0048] <Disinfection device 52> The disinfection device 52 disinfects the water supplied to the accommodation part 11. The disinfection device 52 may be provided outside the accommodation part 11 or may be provided inside the accommodation part 11.

[0049] The disinfection device 52 disinfects, for example, the circulating water supplied to the accommodation part 11 via the circulation pipe 31 or the dilution water 130 supplied to the accommodation part 11 via the water supply pipe 32. At this time, the disinfection device 52 may be provided in the circulation pipe 31 or in the water supply pipe 32.

[0050] The disinfection device 52 uses, for example, a known ultraviolet sterilization and purification device, an ozone generator, a hypochlorous acid water generator, or the like.

[0051] <Bubble generator 53> The bubble generator 53 generates oxygen nanobubble water or the like (for example, including oxygen nanobubble water or oxygen microbubble water) by blowing a gas containing at least oxygen into the water supplied to the housing portion 11. The bubble generator 53 may be provided outside the housing portion 11 or may be provided inside the housing portion 11.

[0052] The bubble generator 53 generates oxygen nanobubble water or the like by blowing a gas containing at least oxygen into the circulating water supplied to the housing portion 11 via the circulation pipe 31 or the dilution water 130 supplied to the housing portion 11 via the water supply pipe 32. At this time, the bubble generator 53 may be provided inside the circulation pipe 31 or the water supply pipe 32.

[0053] The method for generating oxygen nanobubble water or the like by the bubble generator 53 is, for example, pressurizing a gas such as oxygen or air and dissolving it in a supersaturated state in the water supplied to the housing portion 11, and generating nanobubbles or the like (including nanobubbles and microbubbles) in the liquid by a rapid pressure reduction. Oxygen nanobubble water refers to water containing fine bubbles of oxygen gas with a diameter in the nano order (1 μm or less). However, in addition to the fine bubbles of oxygen gas in the nano order, it may contain fine oxygen gas in the micro order (1 to 100 μm). Also, the microbubbles may be floated and separated, and only the nanobubbles may remain in the liquid. Further, the bubble generator 53 may generate oxygen nanobubble water or the like containing oxygen as at least nano-sized fine bubbles including either one or both of oxygen nanobubbles or the like and air nanobubbles or the like. As a specific example of oxygen nanobubble water, about 90% of the bubbles having an average diameter of 50 nm to 100 nm are included among the bubbles having a diameter of 200 nm or less, and the concentration of the bubbles is 2×10^8 / L to 6×10^9 / L.

[0054] As for the details of the bubble generator 53, for example, there are a "swirling flow method" that mixes oxygen gas and water and swirls them at high speed to create oxygen bubbles, a "pressure dissolution method" that applies pressure to oxygen gas, dissolves it in water, and then releases it all at once to create oxygen bubbles, a "fine pore method" that applies pressure to oxygen gas and passes it through fine pores such as orifices to create oxygen bubbles, a "ultrasonic method" that causes cavitation with ultrasonic waves and expands the oxygen gas in water to create oxygen bubbles, a "static mixer type" that swirls and crushes gas in a gas-liquid flow path provided with protrusions to create bubbles, an "ejector type" or "venturi type" that forms a rapid pressure change in the gas-liquid flow path to create bubbles, etc. However, the production method of oxygen nanobubble water etc. is not particularly limited, and any means that can produce nanobubble water etc. containing fine oxygen gas in the nano order or micro order may be used.

[0055] By making oxygen into nano-sized fine bubbles, the T1 relaxation time (the time from when the movement (nuclear spin) of water becomes active due to nuclear magnetization until it returns to a quiet state) is improved compared to normal distilled water, that is, the motility is enhanced, and the mobility of the substances contained in the accommodation part 11 is increased. As a result, it becomes easier for oxygen nanobubble water, the fed bait, marine shrimp A, etc. to come into contact within the accommodation part 11, and the growth of marine shrimp A can be further promoted.

[0056] The acclimation system 1 uses the above-mentioned bubble generator 53. In this case, the weight of marine shrimp A can be efficiently increased. Thereby, it is possible to surely promote growth even in an environment where the salinity concentration is lower than that of seawater for marine shrimp A. The effects of the present invention regarding the acclimation efficiency and growth promotion of marine shrimp A will be described in the examples below.

[0057] <Water quality monitoring device 54> The water quality monitoring device 54 monitors the barometer of the water quality of the breeding water 110. The water quality monitoring device 54 monitors, for example, the dissolved oxygen concentration (mg / L), pH (Potential Hydrogen), NH4 + concentration (mg / L), NO2 - concentration (mg / L), NO3- Monitor the concentration (mg / L), etc. Based on the monitoring results of the water quality monitoring device 54, the administrator of the acclimation system 1 supplies a pH adjuster such as lime water to the storage unit 11 as necessary to adjust the water quality of the breeding water 110.

[0058] As the water quality monitoring device 54, for example, a known automatic water quality monitoring device may be used.

[0059] <Temperature control device 55> The temperature control device 55 controls the water temperature of the breeding water 110. As the temperature control device 55, for example, a known thermostat-built-in heater may be used.

[0060] <Automatic feeding device 56> The automatic feeding device 56 automatically supplies food to the seawater shrimp A in the breeding water 110. As the automatic feeding device 56, a known automatic fish feeder may be used.

[0061] <Lighting unit 57> The lighting unit 57 irradiates the breeding water 110 with green light L. The lighting unit 57 continuously irradiates green light L during the breeding period of the seawater shrimp A. In this case, the weight of the seawater shrimp A can be efficiently increased. As a result, it is possible to surely promote growth even in an environment where the salinity concentration is lower than that of seawater for the seawater shrimp A. The effects of the present invention regarding the acclimation efficiency and growth promotion of the seawater shrimp A will be described in the examples below.

[0062] According to "[New Technology] Growth Promotion of Starry Flounder and Flounder by Irradiation with Green Light Akiyoshi Takahashi, Daisuke Shimizu, Kumiko Tsuru, Kazuto Kizaki, Kanta Mizusawa Monthly Aquanet April 2019 Issue / Offprint", it has been demonstrated that irradiation with a specific color increases the weight of flatfish in general and is effective for efficient growth promotion. As the relationship between the color and the growth promotion effect, the effect of increasing the weight in the order of green, blue-green, and blue was confirmed. Specifically, flounder fry (average weight 20.6 g) were placed in a rectangular concrete on-land aquarium (4.5 m × 4.5 m, water depth about 30 cm) with 600 tails on each of the three sides (accommodation density 30 tails / m 2)The changes in body weight over approximately one year were measured in a control group that was housed and reared under natural light and natural day length, and an irradiated group that was irradiated with green LED light manufactured by Stanley Electric Co., Ltd. (registered trademark) for 12 hours from 6:00 to 18:00. As a result, the average body weight of the irradiated group (776.1 ± 26.2 g) was 61% higher than that of the control group (481.6 ± 18.9 g). Thus, it was confirmed that green light irradiation using LED as a light source can promote the growth of flounder even in water tanks and the like used in fish farms. For the domestication system 1 of the present invention, similar lighting equipment and irradiation conditions may be used.

[0063] <Artificial aquatic plant 6> The artificial aquatic plant 6 is provided in advance in the housing part 11. The artificial aquatic plant 6 can prevent the mixed swimming of each seawater shrimp A in the housing part 11 and prevent cannibalism. Thereby, a decrease in the survival rate of the seawater shrimp A can be suppressed. As the artificial aquatic plant 6, for example, artificial spawning algae made of kinran (registered trademark) (made of vinylon) manufactured by Kyorin Co., Ltd. is used.

[0064] The domestication system 1 of the present invention uses the above-described artificial aquatic plant 6. In this case, the body weight of the seawater shrimp A can be efficiently increased. Thereby, it is possible to surely promote the growth of the seawater shrimp A even in an environment where the salt concentration is lower than that of seawater. The effects of the present invention regarding the domestication efficiency and growth promotion of the seawater shrimp A will be described in the examples below.

[0065] Next, as a method for domesticating the seawater shrimp A in the present embodiment, an example of the operation of the domestication system 1 will be described. In the present embodiment, the operation of the domestication system 1 by the operation of the administrator of the domestication system 1 will be described. However, when the domestication system 1 includes a processing device such as a known PC (Personal Computer) not shown in the figure, the operation of each component of the domestication system 1 may be realized by processing based on a program stored in advance.

[0066] The operation of the domestication system 1 has, for example, a salt concentration reduction step S11 as shown in FIG. 2.

[0067] <Preparations> As a preliminary preparation for the salinity concentration reduction step S11, the administrator stores, for example, as shown in FIG. 3(a), breeding water 111 having a salinity concentration equivalent to seawater in which marine organisms can survive and marine shrimp A in the storage unit 11. As the breeding water 111, natural seawater or artificial seawater having a salinity concentration close to the natural habitat environment of the marine shrimp A is used.

[0068] In addition, the administrator connects a salinity concentration reduction unit 12 to the storage unit 11 that stores the breeding water 111. Specifically, a water supply tank 13 is connected to the storage unit 11 via a water supply pipe 32, and a waste water tank 14 is connected via a drain pipe 33.

[0069] In addition, when using the lighting unit 57 and the artificial waterweed 6, the administrator checks that the lighting unit 57 can irradiate the breeding water 110 with green light L, and that the artificial waterweed 6 is installed in the breeding water 110.

[0070] <Salinity Concentration Reduction Step S11> In the salinity concentration reduction step S11, as shown in FIG. 3(b) for example, the salinity concentration reduction unit 12 reduces the salinity concentration of the breeding water 111 in the storage unit 11 by supplying dilution water 130 and discharging waste water 140. Specifically, the salinity concentration reduction unit 12 supplies dilution water 130 from the water supply tank 13 to the storage unit 11 and discharges the breeding water 111 from the storage unit 11 to the waste water tank 14. As a result, a part of the breeding water 111 in the storage unit 11 is replaced with the dilution water 130, and the breeding water 112 has a lower salinity concentration than the breeding water 111. The breeding water 111 or 112 in the storage unit 11 is repeatedly reduced in salinity concentration by the salinity concentration reduction unit 12 for a predetermined period to obtain breeding water 110 having a salinity concentration of about 0.007 to 0.500 mass%.

[0071] Here, the breeding water 112 refers to water having a salinity concentration between the salinity concentration of the breeding water 111 and the salinity concentration of the dilution water 130. That is, the salinity concentration of the breeding water 112 is lower than the salinity concentration of the breeding water 111 and higher than or equal to the salinity concentration of the breeding water 110.

[0072] Further, the salinity reduction unit 12 may reduce the salinity of the breeding water 111 (breeding water 112) by adjusting the supply amount of the dilution water 130 to the accommodation unit 11 and the discharge amount of the waste water 140 from the accommodation unit 11 to the same flow rate, or by adjusting them to different flow rates. Further, the salinity reduction unit 12 may reduce the salinity of the breeding water 111 (breeding water 112) only by supplying the dilution water 130 without discharging the waste water 140.

[0073] In the salinity reduction step S11, the salinity reduction unit 12 reduces the salinity of the breeding water 111 by 0.01% by mass to 0.09% by mass per day. When the salinity of the breeding water 111 is extremely reduced by 0.09% by mass per day, the survival rate of the marine shrimp A becomes 0%, so the efficiency improvement and growth promotion of the land-based culture of the marine shrimp A cannot be achieved. Further, when the salinity of the breeding water 111 is reduced by less than 0.01% by mass per day, the period for acclimating the marine shrimp A to a low salinity becomes excessively long, and the amount of work such as water replacement increases, so the reliable efficiency improvement and growth promotion of the land-based culture of the marine shrimp A cannot be achieved. For this reason, it is preferable that the salinity reduction unit 12 reduces the salinity of the breeding water 111 by 0.01% by mass to 0.09% by mass per day. In this case, it is possible to efficiently acclimatize to a low-salinity environment while suppressing a decrease in the survival rate of the marine shrimp A. Thereby, the efficiency of the land-based culture of the marine shrimp A can be improved. In addition, the body weight of the marine shrimp A can be efficiently increased. Thereby, reliable efficiency improvement and growth promotion of the land-based culture of the marine shrimp A can be achieved.

[0074] Note that the effect of reducing the salinity of the breeding water 111 by 0.01% by mass to 0.09% by mass per day is particularly remarkable in an environment where the salinity is 0.15% by mass or less or 0.20% by mass or less. For example, when reducing the salinity from 1.50 to 4.00% by mass to 0.15 to 1.00% by mass, even if the salinity is reduced by more than 0.10% by mass per day (for example, the salinity is reduced by about 0.10% by mass to 1.50% by mass per day), the survival rate of the marine shrimp A does not extremely decrease.

[0075] That is, in the salinity reduction step S11, when the salinity is more than 0.20% by mass, the salinity reduction unit 12 reduces the salinity of the breeding water 111 by 0.10% to 1.00% by mass per day, and when the salinity is 0.20% by mass or less, the salinity reduction unit 12 may reduce the salinity of the breeding water 111 by 0.01% to 0.09% by mass per day. In this case, while suppressing the decrease in the survival rate of the seawater shrimp A, it is possible to efficiently acclimatize to a low-salinity environment, and further shorten the period for acclimatizing the seawater shrimp A to a low salinity. Thereby, it is possible to further surely improve the efficiency and promote the growth of the land-based culture of the seawater shrimp A.

[0076] Also, before and after the salinity reduction step S11, the breeding water 111 before salinity reduction, the breeding water 112 during salinity reduction, and the breeding water 110 after salinity reduction all contain, as the mass of the solute per 1 L of the breeding water, 35 to 320 mg of Mg 2+ and 25 to 250 mg of Ca 2+ and 8 to 80 mg of K + and preferably have a first mass ratio (mass of Mg 2+ / mass of K + ) of 0.7 to 5.0, a second mass ratio (mass of Ca 2+ / mass of K + ) of 0.5 to 5.0, and a third mass ratio (mass of Mg 2+ / mass of Ca 2+ ) of 0.7 to 1.4. In this case, the survival rate of the seawater shrimp A is 75% or more, and the survival rate of the seawater shrimp A can be improved. Thereby, it is possible to improve the efficiency of the land-based culture of the seawater shrimp A. Details of the relationship between the breeding water 110 (breeding water 111) and the survival rate of the seawater shrimp A will be described later.

[0077] In addition, in the salt concentration reduction step S11, the bubble generator 53 may blow a gas containing at least oxygen into the water supplied to the accommodation section 11 via, for example, the circulation pipe 31 or the water supply pipe 32. At this time, oxygen nanobubbles or the like are generated in the breeding water 110 (breeding water 111, breeding water 112). As a result, oxygen nanobubble water or the like can be generated from the breeding water 110. Note that a step of generating oxygen nanobubble water, which is distinguished from the salt concentration reduction step S11, may be carried out before or after the salt concentration reduction step S11, or may be carried out a plurality of times.

[0078] By performing the above-described steps, the operation of the acclimation system 1 in the present embodiment ends. Note that the acclimation system 1 may, for example, repeatedly perform the above-described steps.

[0079] According to the present embodiment, there is provided a salt concentration reduction step S11 for reducing the salt concentration of the breeding water 111 in which the marine shrimp A is bred by 0.01% by mass to 0.09% by mass per day. Therefore, it is possible to efficiently acclimate the shrimp to a low-salt environment while suppressing a decrease in the survival rate of the marine shrimp A. As a result, it is possible to surely improve the efficiency and promote the growth of the land-based aquaculture of the marine shrimp A.

[0080] (Growth method by aquaponics 2) With reference to FIGS. 4 to 5, an example of a growth method by aquaponics 2 using the marine shrimp A acclimated by the shrimp acclimation method in the present embodiment will be described. Note that the description of the configuration similar to the content of the above-described shrimp acclimation method will be omitted. First, the aquaponics 2 used in the growth method by the aquaponics 2 of the present embodiment will be described.

[0081] <Aquaponics 2> As shown in FIG. 4 for example, aquaponics 2 includes the above-described acclimation system 1, a hydroponic tank 21 that houses plant B, a decomposition liquid generation unit 22, and a decomposition liquid supply unit 23. In this embodiment, an example of a closed circulation type in which aquaponics 2 circulates and uses the breeding water 110 in the accommodation unit 11 in the hydroponic tank 21 will be described, but a flow-through type in which the breeding water 110 in the accommodation unit 11 is used in the hydroponic tank 21 and then becomes wastewater may also be used.

[0082] <accommodation unit 11> The accommodation unit 11 is connected to the hydroponic tank 21 via, for example, a first circulation pipe 31a and a second circulation pipe 31b. The accommodation unit 11 supplies the breeding water 110 to the hydroponic tank 21 via, for example, the first circulation pipe 31a. The accommodation unit 11 may be supplied with the drainage discharged from the hydroponic tank 21 via, for example, the second circulation pipe 31b. As the first circulation pipe 31a and the second circulation pipe 31b, for example, water pipes of the same type as the circulation pipe 31 may be used.

[0083] <hydroponic tank 21> The hydroponic tank 21 houses plant B. The hydroponic tank 21 is supplied with the breeding water 110 containing the excrement of the marine shrimp A from the accommodation unit 11 via the first circulation pipe 31a. The hydroponic tank 21 supplies the breeding water and the like (including the breeding water 210 and the decomposition liquid 220 described later) in the hydroponic tank 21 to the accommodation unit 11 via the second circulation pipe 31b.

[0084] The hydroponic tank 21 may be supplied with the breeding water 110 that does not contain the excrement of the marine shrimp A together with the breeding water 110 that contains the excrement of the marine shrimp A. Here, the breeding water 110 supplied to the hydroponic tank 21 is referred to as breeding water 210. The breeding water 210 is, for example, of the same type as the breeding water 110.

[0085] <decomposition liquid generation unit 22> The decomposition liquid generation unit 22 generates a decomposition liquid 220 by biodegrading the excrement of the marine shrimp A, as shown in FIG. 4 for example. The decomposition liquid generation unit 22 is provided, for example, inside the hydroponic tank 21.

[0086] The decomposition liquid generation unit 22 is, for example, a carrier for immobilizing microorganisms, and generates a decomposition liquid 220 by biodegrading the excrement of the seawater shrimp A contained in the breeding water 210 in the hydroponic cultivation tank 21 through the decomposition action of microorganisms (not shown). Examples of the microorganisms immobilized in the decomposition liquid generation unit 22 include nitrifying bacteria. The decomposition liquid generation unit 22 may be provided, for example, in the housing unit 11.

[0087] <Decomposition liquid supply unit 23> The decomposition liquid supply unit 23 supplies the decomposition liquid 220 to the plant B, for example. The decomposition liquid supply unit 23 is provided in the hydroponic cultivation tank 21, for example. In this case, the weight of the seawater shrimp A and the weight of the plant B can be efficiently increased. Thereby, it is possible to surely promote the growth of the seawater shrimp A and the plant B that is vulnerable to salt damage at the same time. The utility of the present invention related to the growth promotion of the plant B will be described in the examples below.

[0088] The decomposition liquid supply unit 23 is, for example, a base such as a sponge for fixing the plant B in the hydroponic cultivation tank 21. The decomposition liquid supply unit 23 supplies at least one of the breeding water 210 and the decomposition liquid 211 to the plant B. When a porous material such as a sponge is used for the decomposition liquid supply unit 23, the material may also function as the decomposition liquid generation unit 22 by carrying microorganisms such as nitrifying bacteria.

[0089] <Plant B> The plant B is housed in the hydroponic cultivation tank 21. The plant B is fixed in the hydroponic cultivation tank 21 by the decomposition liquid supply unit 23 composed of a base such as a sponge, for example. As the plant B, a plant that can be used for hydroponics using the breeding water 110 with a reduced salt concentration by the salt concentration reduction unit 12, for example, is selected. Therefore, in aquaponics using marine organisms such as the seawater shrimp A, halophytes such as ice plants have been exclusively cultivated in the past, but according to the present invention, plants B other than halophytes such as ice plants can also be cultivated.

[0090] Examples of Plant B include Asteraceae plants (such as leaf lettuce), Amaranthaceae plants (such as spinach and Swiss chard), Alliaceae plants (such as garlic chives), Apiaceae plants (such as parsley), Brassicaceae plants (such as cress and wasabi greens), Lamiaceae (such as sweet basil), Solanaceae (such as chili peppers, bell peppers, and eggplants), and Alliaceae (such as scallions).

[0091] Next, as a cultivation method by Aquaponics 2 in this embodiment, an example of the operation of Aquaponics 2 will be described. Aquaponics 2 operates each component based on, for example, an operation by an administrator or a program stored in advance. The operation of Aquaponics 2 has, for example, as shown in FIG. 5, a salinity reduction step S11, a decomposition liquid generation step S21, and a decomposition liquid supply step S22. When the salinity reduction step S11 is performed by another acclimation system 1' independent of the acclimation system 1 constituting Aquaponics 2, the salinity reduction step S11 may be omitted.

[0092] Before the decomposition liquid generation step S21, Aquaponics 2 drains the breeding water 110 containing the excrement of the seawater shrimp A from the housing unit 11 and supplies breeding water 210 of the same quality as the breeding water 110 to the hydroponic cultivation tank 21, for example, as shown in FIG. 4.

[0093] <Decomposition Liquid Generation Step S21> In the decomposition liquid generation step S21, the decomposition liquid generation unit 22 biodegrades the excrement of the seawater shrimp A in the breeding water 210 to generate a decomposition liquid 220.

[0094] <Decomposition Liquid Supply Step S22> In the decomposition liquid supply step S22, the decomposition liquid supply unit 23 supplies, for example, the decomposition liquid 220 generated by the decomposition liquid generation step S21 to Plant B. In this case, the weight of the seawater shrimp A and the weight of Plant B can be efficiently increased. Thereby, it is possible to surely promote the growth of both the seawater shrimp A and the salt-sensitive Plant B at the same time.

[0095] According to this embodiment, there are provided a salinity concentration reduction step S11 of reducing the salinity concentration of the breeding water 111 in which the marine shrimp A is bred by 0.01% by mass to 0.09% by mass per day, a decomposition liquid generation step S21 of biodegrading the excrement of the marine shrimp A to generate a decomposition liquid 220, and a decomposition liquid supply step S22 of supplying the generated decomposition liquid 220 to the plant B hydroponically cultivated using the breeding water 110 with the reduced salinity concentration. Therefore, it is possible to efficiently acclimatize the marine shrimp A to a low-salinity environment while suppressing a decrease in the survival rate of the marine shrimp A, and further efficiently increase the weight of the plant B. Thereby, it is possible to improve the efficiency of land-based aquaculture for the marine shrimp A and more surely promote growth, while improving the efficiency of plant cultivation.

Example

[0096] Hereinafter, the examples and comparative examples of the present invention when the above-described embodiment is used will be specifically described.

[0097] <Experiment 1: Acclimation efficiency of marine shrimp A for each reduction amount of salinity concentration in breeding water> In this experiment, the acclimation efficiency of the marine shrimp A was confirmed by comparing the survival rate of the marine shrimp A during the salinity concentration reduction period for each reduction amount of the salinity concentration of the breeding water in which the marine shrimp A was bred per day. As the survival rate, the survival rate at the time when the salinity concentration continued to decrease and reached 0.01% by mass was measured. In addition, the body weight and body length of the marine shrimp A were also measured.

[0098] In this experiment, Pacific white shrimp was used as the marine shrimp A. Specifically, juvenile shrimp less than 1 month after hatching, with an average body length of about 1.000 cm and an average body weight of about 0.020 g were used. The number of Pacific white shrimp used in each experiment was 2000 per one type of condition (one test plot). As the housing unit 11, a 2000 L water tank that houses 2000 Pacific white shrimp was used.

[0099] As a method for measuring the average body length of Pacific white shrimp in this experiment, the length from the tip of the mouth to the tail of each Pacific white shrimp was measured with a ruler, and the total value of the body lengths of each individual was divided by the number of individuals to derive the average body length. As a method for measuring the average body weight of Pacific white shrimp, the total weight of the Pacific white shrimp, the housing part 11, and the breeding water 110, and the weight of the housing part 11 and the breeding water 110 were measured with an electronic balance (TX423N). The weight of the housing part 11 and the breeding water 110 was subtracted from the total weight, and the difference was divided by the number of individuals in the housing part 11 to derive the average body weight.

[0100] As a method for measuring the salinity concentration, the sensor part of the salinity meter "7IN1 Water quarlity tester" manufactured by wanbang ep tech, which can measure in units of 0.001% with a salinity concentration measurement range of 0 to 25%, was immersed in the breeding water 110 in the housing part 11 for measurement. As a method for measuring the dissolved oxygen content, a dissolved oxygen meter "AR8406" was used. As a method for measuring the pH, a pH meter manufactured by Toa DKK Corporation (registered trademark) was used.

[0101] As a method for feeding seawater shrimp A, an automatic feeder "EV500" manufactured by EVNICE fish feeder was used. As feed, for seawater shrimp A with a body length of 6 cm or less, feed with a protein content of about 38% by mass or more based on the total mass was supplied. Similarly, for seawater shrimp A with a body length of more than 6 cm and less than 10 cm, feed with a protein content of about 36 to 38% by mass based on the total mass was supplied, and for seawater shrimp A with a body length of 10 cm or more, feed with a protein content of about 32 to 36% by mass based on the total mass was supplied. In addition, since the salinity reduction period in this experiment corresponds to the juvenile shrimp stage of seawater shrimp A and is one month from the start of aquaculture, as an appropriate feeding amount for juvenile shrimp, feed equivalent to 5% of the body weight of seawater shrimp A per day was supplied in more than 9 portions per day.

[0102] As the breeding environment for seawater shrimp A, the breeding density was 5 kg / m 3 , the water temperature of the breeding water 110 was 28 to 32 °C, the dissolved oxygen content of the breeding water 110 was 6.5 to 10.0 mg / L, the pH of the breeding water 110 was 6.8 to 8.2, and the NH4 of the breeding water 110 +The concentration was adjusted to 0.0 - 0.5 (mg / L) for NO2 in the breeding water 110 - The concentration was adjusted to 0.0 - 0.5 (mg / L) for NO3 in the breeding water 110 - The concentration was adjusted to 100 (mg / L) or less. In addition, the breeding water 110 was circulated at 40 L / min using a water pump manufactured by Eheim GmbH & Co. KG, and air blowing was performed using an air pump "AP - 100F". As a temperature control method inside the accommodation section 11, an aquarium cooler "ZR - 250" was used as the temperature control device 55. Regarding the breeding water 111 before reducing the salt concentration, the breeding density, water temperature, dissolved oxygen amount, pH, and each ion concentration were adjusted to the same level as the breeding water 110

[0103] As the main components of the breeding water 110, potassium sulfate (K2SO4) and magnesium sulfate (MgSO4) manufactured by Fuji Film Wako Pure Chemical Corporation were added to pure water in which calcium ions, magnesium ions, potassium ions, and sulfate ions were all 0 mg / L, so that the magnesium ion was about 40 mg / L - about 240 mg / L, the calcium ion was about 30 mg / L - about 200 mg / L, the potassium ion was about 10 mg / L - about 60 mg / L, the sulfate ion was about 70 mg / L - about 400 mg / L, and the strontium ion was about 8 mg / L. Regarding the breeding water 111 before reducing the salt concentration, the main components were adjusted to the same level as the breeding water 110. Also, using artificial seawater with a salt concentration of about 3.2% as the breeding water 111, the breeding water 110 was obtained by reducing the salt concentration to about 0.007% - 0.500%. As a method for reducing the salt concentration of the breeding water 111, a part of the breeding water 111 inside the accommodation section 11 was drained, and approximately the same amount of groundwater (fresh water) was supplied to reduce the salt concentration of the breeding water 111 inside the accommodation section 11. The operations of draining the breeding water 111 and supplying groundwater were intermittent operations, for example, continuous water supply and drainage for about 30 minutes per hour

[0104] In this experiment, for the rearing water 111, in the range where the salt concentration is 0.15 mass% or more, the salt concentration was reduced by 0.15 to 1.50 mass% per day, and then in the range where the salt concentration is 0.15 mass% or less, it was "reduced by 0.01 to 0.05 mass% per day (Example 1 of the present invention)", and the salt concentration of the final rearing water 110 was reduced to 0.01 mass%. Separately from Example 1 of the present invention, in the range where the salt concentration is 0.20 mass% or more, the salt concentration was reduced by 0.30 to 1.00 mass% per day, and then in the range where the salt concentration is 0.20 mass% or less, by three patterns of salt concentration reduction methods, the salt concentration of the final rearing water 110 was reduced to 0.01 mass%. Specifically, it was set as "reduced by 0.02 to 0.03 mass% per day (Example 2 of the present invention)", "reduced by 0.05 to 0.09 mass% per day (Example 3 of the present invention)", and "reduced by 0.10 mass% per day (Comparative Example 1)".

[0105] Among the results of this experiment, the results of Example 1 of the present invention are as shown in Table 1. According to Table 1, in Example 2 of the present invention, the salt concentration reduction period was 13 days, and the survival rate of the banana prawns on the 13th day was 75%. Also, the body weight of the banana prawns increased from 0.020 g per individual to 1.095 g per individual, and the body length increased from 1.000 cm per individual to 5.500 cm per individual.

[0106]

Table 1

[0107] Among the results of this experiment, the results of Example 2 of the present invention are as shown in Table 2. According to Table 2, in Example 2 of the present invention, the salt concentration reduction period was 14 days, and the survival rate of the banana prawns on the 14th day was 80%. Also, the body weight of the banana prawns increased from 0.020 g per individual to 1.185 g per individual, and the body length increased from 1.000 cm per individual to 6.000 cm per individual.

[0108]

Table 2

[0109] Among the results of this experiment, the results of Invention Example 3 are as shown in Table 3. According to Table 3, in Invention Example 3, the salt concentration reduction period was 9 days, and the survival rate of whiteleg shrimp on the 9th day was 85%. Also, the weight of the whiteleg shrimp increased from 0.020 g per shrimp to 0.737 g per shrimp, and the body length increased from 1.000 cm per shrimp to 3.000 cm per shrimp.

[0110]

Table 3

[0111] Among the results of this experiment, the results of Comparative Example 1 are as shown in Table 4. According to Table 4, in Comparative Example 1, the salt concentration reduction period was 8 days, and the survival rate of whiteleg shrimp on the 8th day was 0%.

[0112]

Table 4

[0113] According to Tables 1 to 3, as a result of setting the daily salt concentration reduction amount of the breeding water 111 to 0.01 to 0.09 mass%, it was possible to efficiently acclimatize the whiteleg shrimp to survive in a low-salt environment while suppressing the decrease in the survival rate. Therefore, it was possible to efficiently acclimatize the marine shrimp A to a low-salt environment. As a result, it is possible to surely improve the efficiency and promote the growth of the land-based culture of the marine shrimp A.

[0114] Also, according to Table 4, as a result of setting the daily salt concentration reduction amount of the breeding water 111 to 0.10 mass% per day, the survival rate of the whiteleg shrimp became 0%. Therefore, it is not possible to surely improve the efficiency and promote the growth of the land-based culture of the marine shrimp A.

[0115] <Experiment 2: Promotion of the growth of Plant B> Next, a verification experiment regarding the growth promotion effect of Plant B for each salinity concentration of the breeding water 110 used in the aquaponics 2 according to the present invention will be described. Specifically, the average fresh weight of the edible part and the average size of the edible part of Plant B grown using the breeding water 110 and seawater (salinity concentration of about 3% by mass) were measured at the 35th day of the growth period, and the growth promotion effect of Plant B was confirmed particularly based on the weight increase amount. Further, as a comparative example, the average fresh weight of the edible part and the average size of the edible part of Plant B grown using liquid fertilizer in a single hydroponic tank not connected to the housing part 11 were also measured at the 35th day of the growth period. The 35-day growth period of Plant B is after the salinity reduction period, but groundwater was added to maintain the water volume when the water volume decreased due to water absorption and evaporation by Plant B. For this reason, the salinity concentration can still decrease even after the salinity reduction period.

[0116] In this experimental example, when reducing the salinity concentration from the breeding water 111 to the breeding water 110, the salinity reduction amount was reduced under the condition of 0.10% by mass or more per day. Therefore, although described as "reference example" and "comparative example", it goes without saying that the same results are obtained when the salinity concentration is reduced by 0.01 to 0.09% by mass per day by the salinity reduction step S11.

[0117] In this experiment, Litopenaeus vannamei was used as the seawater shrimp A. Specifically, juvenile shrimp with an average body length of about 0.8 cm and an average body weight of about 0.2 g less than 1 month after hatching were used. The number of Litopenaeus vannamei used in each experiment was 2000 per one type of condition (one test plot). As the housing part 11, a 2000 L water tank for housing 2000 Litopenaeus vannamei was used.

[0118] Further, as Plant B, Japanese bunching onion and sweet basil, which were confirmed to be able to be grown by supplying the breeding water 110 etc. whose temperature was adjusted to 25 to 34 °C for the breeding environment temperature of the seawater shrimp A to the Plant B in the hydroponic tank 21 without temperature adjustment, were used. The number of Japanese bunching onion and sweet basil used in each experiment was 150 per one type of condition (one test plot).

[0119] For the single hydroponic cultivation tank using liquid fertilizer, the water temperature was adjusted to about 26 to 30°C. The single hydroponic cultivation tank adopted a form that combined the NFT method and the DWC method. As the liquid fertilizer, the chemical liquid fertilizer Just One (registered trademark) was used. In the hydroponic cultivation tank 21 in aquaponics 2, it is rich in the feed for marine shrimp A and calcium ions (Ca 2+ ) and magnesium ions (Mg 2+ ) contained in the pH adjuster, as well as the components decomposed by the microorganisms carried in the hydroponic cultivation tank 21. On the other hand, these are not richly contained in the commercially available liquid fertilizer used in the single hydroponic cultivation tank, so the components of the water used for cultivation are different.

[0120] As the method for measuring the average fresh weight of the edible parts of Chinese chives and sweet basil in this experiment, for 10 plants randomly selected from the total number of plants, the average value of the fresh weight of the edible parts (stems and leaves) was calculated. Similarly, for the average height of the edible parts, for 10 plants randomly selected from the total number of plants, the average value of the height of the edible parts (stems and leaves) was calculated.

[0121] The salt concentrations of the breeding water 110 compared in this experiment were "0.007 to 0.200 mass%" (Reference Example 1), "0.300 mass%" (Reference Example 2), "0.500 mass%" (Reference Example 3), and "3 mass%" (Comparative Example 2). Among these, the salt concentrations of 0.007 to 0.50 mass% according to the present invention were used as Reference Example 1 to Reference Example 3, respectively. Also, the example cultivated using seawater with a salt concentration of about 3 mass% was used as Comparative Example 2. In addition, the example cultivated using liquid fertilizer in a single hydroponic cultivation tank was used as Comparative Example 3. Incidentally, in the range of the salt concentration of "0.007 to 0.200 mass%", there was no change in the average fresh weight and average size of the edible parts of Chinese chives and sweet basil.

[0122] The results of this experiment are as shown in Table 5.

[0123]

Table 5

[0124] According to Table 5, the average fresh weight of the edible parts of Chinese chives in each example was 8.0 g / plant for Reference Example 1, 7.8 g / plant for Reference Example 2, 6.5 g / plant for Reference Example 3, and 4.0 g / plant for Comparative Example 3. The average size of the edible parts of Chinese chives in each example was 26.0 cm / plant for Reference Example 1, 23.0 cm / plant for Reference Example 2, 20.0 cm / plant for Reference Example 3, and 18.0 cm / plant for Comparative Example 3. The average fresh weight of the edible parts of sweet basil in each example was 163.0 g / plant for Reference Example 1, 106.0 g / plant for Reference Example 2, 99.0 g / plant for Reference Example 3, and 98.0 g / plant for Comparative Example 3. The average size of the edible parts of sweet basil in each example was 42.0 cm / plant for Reference Example 1, 39.0 cm / plant for Reference Example 2, 36.0 cm / plant for Reference Example 3, and 36.0 cm / plant for Comparative Example 3. Note that cultivation was not possible for Comparative Example 2.

[0125] In Reference Examples 1 to 3, the salt concentration of the breeding water 110 was set to 0.007 to 0.500% by mass. As a result, it was confirmed that the average fresh weight of the edible parts of Chinese chives and sweet basil was higher than that of Chinese chives and sweet basil grown with seawater and liquid fertilizer. Therefore, the body weight of seawater shrimp A and the weight of plant B can be efficiently increased. Thereby, it is possible to surely promote the growth of seawater shrimp A while simultaneously promoting the growth of plant B that is vulnerable to salt damage or plant B that can grow under fresh water.

[0126] Also, when comparing the average fresh weights of the edible parts of Reference Examples 1 to 3, it was confirmed that they were higher in the order of Reference Example 1, Reference Example 2, and Reference Example 3. Therefore, by using breeding water 110 with a lower salt concentration, the weight of plant B can be increased more efficiently.

[0127] Also, according to Table 5, it was similarly confirmed that the average size of the edible parts of Chinese chives and sweet basil in Reference Examples 1 to 3 was larger than that of Chinese chives and sweet basil grown with seawater and liquid fertilizer. Therefore, the body length of seawater shrimp A and the body length of plant B can be efficiently increased. Thereby, it is possible to more surely promote the growth of seawater shrimp A while simultaneously promoting the growth of plant B that is vulnerable to salt damage or plant B that can grow under fresh water.

[0128] In Comparative Example 2, plant B was grown using seawater with a salt concentration of about 3% by mass. As a result, Chinese chives and sweet basil could not be grown. Therefore, the weight of plant B could not be efficiently increased.

[0129] In Comparative Example 3, plant B was grown using liquid fertilizer in a single hydroponic tank. As a result, the average fresh weight and average size of the edible parts of Chinese chives and sweet basil were lower than those when aquaponics 2 was used. Therefore, the weight of plant B could not be efficiently increased.

[0130] That is, the salt concentration of the breeding water 111 suitable for surely promoting the growth of seawater shrimp A and plant B simultaneously is 0.007 to 0.500% by mass, more preferably 0.007 to 0.300% by mass, and still more preferably 0.007 to 0.200% by mass.

[0131] <Experiment 3: Growth promotion effect of seawater shrimp A and plant B related to the presence or absence of green light and artificial waterweed> Next, regarding the aquaponics 2 according to the present invention, a verification experiment on the utility of promoting the growth of seawater shrimp A and plant B accompanying the irradiation of green light on the breeding water 110, the generation of oxygen nanobubble water, and the installation of artificial waterweed will be described.

[0132] In this experimental example, when reducing the salt concentration from the breeding water 111 to the breeding water 110, the reduction amount of the salt concentration was reduced under the condition of 0.10% by mass or more per day. Therefore, although it will be described as a "reference example" and a "comparative example", it goes without saying that the same results will be obtained when the salt concentration is reduced by 0.01 to 0.09% by mass per day by the salt concentration reduction step S11.

[0133] Specifically, the average weight and average body length of domesticated seawater shrimp A on the 75th, 80th, and 90th days of the growth period without green light irradiation, generation of oxygen nanobubble water, and installation of aquatic plants (control group), and the average weight and average body length of domesticated seawater shrimp A on the 75th, 80th, and 90th days of the growth period while performing green light irradiation, generation of oxygen nanobubble water, and installation of aquatic plants in addition to the domestication method of Experiment 1 above were used to calculate the weight increase and body length increase. In particular, the growth promotion effect of seawater shrimp A was confirmed based on the weight increase.

[0134] Also, similar to Experiment 2, the average fresh weight and average size of the edible part of Plant B grown without green light irradiation, generation of oxygen nanobubble water, and installation of artificial aquatic plants on the 20th and 35th days of the growth period (control group), and the average fresh weight and average size of the edible part of Plant B grown while performing green light irradiation, generation of oxygen nanobubble water, and installation of artificial aquatic plants on the 20th and 35th days of the growth period were used to calculate the respective increase amounts. In particular, the growth promotion effect of Plant B was confirmed based on the weight increase.

[0135] In addition, the survival rates of domesticated seawater shrimp A after the end of the salt concentration reduction period and on the 90th day of the growth period without green light irradiation, generation of oxygen nanobubble water, and installation of artificial aquatic plants (control group), and the survival rates of domesticated seawater shrimp A after the end of the salt concentration reduction period and on the 90th day of the growth period while performing green light irradiation, generation of oxygen nanobubble water, and installation of artificial aquatic plants were used to calculate the respective increase amounts, and the survival rate improvement effect of seawater shrimp A was confirmed.

[0136] In this experiment, banana shrimp was used as seawater shrimp A and Chinese chives were used as Plant B. Also, the number of banana shrimp used in each experiment was 100 per one type of condition (one test group). In addition, a 100 L water tank capable of accommodating them was used as the accommodation unit 11. The number of Chinese chives used in each experiment was 30 plants per one type of condition (one test group).

[0137] Regarding the salinity concentration in this experiment, the reduction amount of the salinity concentration of the breeding water 112 was set to 0.20% by mass per day, and the salinity concentration of the breeding water 110 after the completion of the salinity concentration reduction was set to 0.20% by mass.

[0138] Also, in this experiment, green light L was irradiated onto the breeding water 110 (breeding water 111, breeding water 112) using the lighting unit 57 installed above the accommodation unit 11. As the lighting unit 57, "LED work light 72W green" manufactured by CREE was used. As the irradiation conditions, green light L was constantly irradiated during the growth period.

[0139] Also, in this experiment, air was blown into the breeding water 110 (breeding water 111, breeding water 112) using the bubble generator 53 connected to the accommodation unit 11 to generate oxygen nanobubble water. As the bubble generator 53, a known nanobubble generator capable of corresponding to a salinity concentration of about 0.20% by mass to about 3.00% by mass was used. As the generation conditions of the oxygen nanobubble water, the bubble generator 53 was constantly operated during the growth period.

[0140] Also, in this experiment, a plurality of artificial waterweeds 6 were installed on the inner bottom surface of the accommodation unit 11 at intervals of about 1 m. As the artificial waterweeds 6, "Artificial spawning algae" manufactured by Kyorin Co., Ltd. was used.

[0141] Also, for each experiment, the experiment was conducted twice for reproducibility confirmation. Note that other experimental conditions are the same as those in Experiment 2.

[0142] Regarding this experiment, the results of the verification experiment of the seawater shrimp A are as shown in Table 6. Reference Example 4 shows a control group in which neither green light irradiation nor oxygen nanobubble water generation is performed. Reference Example 5 shows an experimental group in which green light irradiation is performed and oxygen nanobubble water generation is not performed. Reference Example 6 shows an experimental group in which oxygen nanobubble water generation is performed and green light generation is not performed. Reference Example 7 shows a control group in which both green light irradiation and oxygen nanobubble water generation are performed. Note that artificial waterweeds were installed in all groups.

[0143]

Table 6

[0144] According to Table 6, the weight gain of the banana prawns in each example compared to Reference Example 4 (control group) on the 75th day of the cultivation period was 5.0 g / prawn for Reference Example 5, 4.7 g / prawn for Reference Example 6, and 10.0 g / prawn for Reference Example 7. Also, the weight gain of the banana prawns in each example compared to Reference Example 4 (control group) on the 80th day of the cultivation period was 6.2 g / prawn for Reference Example 5, 6.0 g / prawn for Reference Example 6, and 9.6 g / prawn for Reference Example 7. Further, the weight gain of the banana prawns in each example compared to Reference Example 4 (control group) on the 90th day of the cultivation period was 5.9 g / prawn for Reference Example 5, 4.7 g / prawn for Reference Example 6, and 8.0 g / prawn for Reference Example 7.

[0145] The body length increase of the banana prawns in each example compared to Reference Example 4 (control group) on the 75th day of the cultivation period was 4.1 cm / prawn for Reference Example 5, 3.8 cm / prawn for Reference Example 6, and 5.8 cm / prawn for Reference Example 7. Also, the body length increase of the banana prawns in each example compared to Reference Example 4 (control group) on the 80th day of the cultivation period was 3.9 cm / prawn for Reference Example 5, 3.7 cm / prawn for Reference Example 6, and 5.2 cm / prawn for Reference Example 7. Further, the body length increase of the banana prawns in each example compared to Reference Example 4 (control group) on the 90th day of the cultivation period was 4.8 cm / prawn for Reference Example 5, 4.2 cm / prawn for Reference Example 6, and 5.9 cm / prawn for Reference Example 7.

[0146] In Reference Example 5, green light L was irradiated onto the breeding water 110 through the lighting unit 57. As a result, it was confirmed that the weight gain of the average weight of the banana prawns was higher than that of the average weight of the banana prawns grown in an environment without irradiation of green light L. Therefore, the weight of the seawater prawn A can be efficiently increased compared to the case without irradiation of green light L. Also, the body length increase showed a similar tendency. Thereby, further growth promotion of the seawater prawn A can be surely achieved.

[0147] In Reference Example 6, air was blown into the breeding water 110 through the bubble generator 53 to generate oxygen nanobubble water. As a result, it was confirmed that the weight increase amount of the average weight of the banana prawns was higher than that of the average weight of the banana prawns grown in an environment where oxygen nanobubble water was not generated. Therefore, the weight of the seawater shrimp A can be efficiently increased as compared with the case where oxygen nanobubble water is not generated. Also, the same tendency was observed for the body length increase amount. Thereby, further growth promotion of the seawater shrimp A can be surely achieved.

[0148] In Reference Example 7, irradiation with green light L and generation of oxygen nanobubble water were performed. As a result, it was confirmed that the weight increase amount of the average weight of the banana prawns was higher than that of the average weight of the banana prawns grown in an environment where green light L was irradiated and oxygen nanobubble water was not generated. Therefore, the weight of the seawater shrimp A can be efficiently increased as compared with the case where green light L is irradiated and oxygen nanobubble water is not generated. Also, the same tendency was observed for the body length increase amount. Thereby, further growth promotion of the seawater shrimp A can be surely achieved.

[0149] Also, regarding this experiment, the results of the verification experiment of Plant B are as shown in Table 7. Note that artificial waterweeds were installed in each section.

[0150]

Table 7

[0151] According to Table 7, the average fresh weight increase amount of the edible part of Chinese chives in each Example with respect to Reference Example 4 (control group) on the 20th day of the cultivation period was 0.0 g / plant for Reference Example 5, 4.0 g / plant for Reference Example 6, and 4.0 g / plant for Reference Example 7. Also, the average fresh weight increase amount of the edible part of Chinese chives in each Example with respect to Reference Example 4 (control group) on the 35th day of the cultivation period was 0.0 g / plant for Reference Example 5, 3.0 g / plant for Reference Example 6, and 4.0 g / plant for Reference Example 7.

[0152] On the 20th day of the cultivation period, the increase in the average size of the edible part of Chinese chives in each example compared to Reference Example 4 (control group) was 1.0 cm / plant for Reference Example 5, 11.0 cm / plant for Reference Example 6, and 12.0 cm / plant for Reference Example 7. Also, on the 20th day of the cultivation period, the increase in the average size of the edible part of Chinese chives in each example compared to Reference Example 4 (control group) was 0.0 cm / plant for Reference Example 5, 6.0 cm / plant for Reference Example 6, and 7.0 cm / plant for Reference Example 7.

[0153] In Reference Example 5, green light L was irradiated onto the breeding water 110 through the lighting unit 57. As a result, it was confirmed that the average fresh weight and the average size of the edible part of Chinese chives were not different from those of Chinese chives grown in an environment where green light L was not irradiated. This is presumably because the green light L was irradiated only onto the breeding water 110 and not onto the plant B.

[0154] In Reference Example 6, air was blown into the breeding water 110 through the bubble generator 53 to generate oxygen nanobubble water. As a result, it was confirmed that the average fresh weight of the edible part of Chinese chives was higher than that of Chinese chives grown in an environment where oxygen nanobubble water was not generated. Therefore, the weight of the plant B can be efficiently increased compared to the case where oxygen nanobubble water is not generated. Also, the average size of the edible part showed a similar tendency. Thereby, further growth promotion of the plant B can be surely achieved.

[0155] In Reference Example 7, irradiation of green light L and generation of oxygen nanobubble water were performed. As a result, it was confirmed that the average fresh weight of the edible part of Chinese chives was higher than that of Chinese chives grown in an environment where oxygen nanobubble water was generated without irradiating green light L. Therefore, the weight of the plant B can be efficiently increased compared to the case where green light L is irradiated and oxygen nanobubble water is not generated. Also, the average size of the edible part showed a similar tendency. Thereby, further growth promotion of the plant B can be surely achieved.

[0156] In addition, for this experiment, the results of the verification experiment of the seawater shrimp A with the installation of the artificial waterweed 6 are as shown in Table 8. Reference Example 8 shows a control group where neither green light irradiation, generation of oxygen nanobubbles, nor installation of artificial waterweed is carried out. Reference Example 9 shows an experimental group where artificial waterweed is installed, but green light irradiation and generation of oxygen nanobubbles are not carried out. Reference Example 10 shows an experimental group where artificial waterweed and green light irradiation are carried out, but generation of oxygen nanobubbles is not carried out. Reference Example 11 shows an experimental group where artificial waterweed and generation of oxygen nanobubbles are carried out, but green light irradiation is not carried out. Reference Example 12 shows a control group where green light irradiation, oxygen nanobubbles, and installation of artificial waterweed are all carried out.

[0157]

Table 8

[0158] According to Table 8, the increase in the survival rate of the banana prawns in each example compared to Reference Example 8 (control group) at the end of the salt concentration reduction period was 27% for all of Reference Examples 9 to 12. Also, the increase in the survival rate of the banana prawns in each example compared to Reference Example 8 (control group) on the 90th day of the growth period was 20% for Reference Example 9, 21% for Reference Example 10, 22% for Reference Example 11, and 25% for Reference Example 12.

[0159] In Reference Example 9, the artificial waterweed 6 was installed in the accommodation part 11. As a result, it was confirmed that the survival rate of the banana prawns was higher than that of the banana prawns grown in an environment without the installation of the artificial waterweed 6. Therefore, the survival rate of the seawater shrimp A can be improved compared to the case where the artificial waterweed 6 is not installed. Thereby, further improvement in the efficiency of land-based aquaculture for the seawater shrimp A can be achieved.

[0160] In Reference Example 10, after installing the artificial waterweed 6 in the housing part 11, green light L was irradiated onto the breeding water 110 through the lighting part 57. As a result, it was confirmed that the survival rate of the banana prawns was higher than that of the banana prawns grown in an environment where green light L was not irradiated. Therefore, the survival rate of the seawater shrimp A can be improved as compared with the case where green light L is not irradiated. Thereby, further improvement in the efficiency of land-based aquaculture of the seawater shrimp A can be achieved.

[0161] In Reference Example 11, after installing the artificial waterweed 6 in the housing part 11, air was blown into the breeding water 110 through the bubble generator 53 to generate oxygen nanobubble water. As a result, it was confirmed that the survival rate of the banana prawns was higher than that of the banana prawns grown in an environment where oxygen nanobubble water was not generated. Therefore, the survival rate of the seawater shrimp A can be improved as compared with the case where oxygen nanobubble water is not generated. Thereby, further improvement in the efficiency of land-based aquaculture of the seawater shrimp A can be achieved.

[0162] In Reference Example 12, after installing the artificial waterweed 6 in the housing part 11, irradiation with green light L and generation of oxygen nanobubble water were performed. As a result, it was confirmed that the survival rate of the banana prawns was higher than that of the banana prawns grown in an environment where either one of the irradiation with green light L and the generation of oxygen nanobubble water was performed. Therefore, the survival rate of the seawater shrimp A can be improved as compared with the case where either one of the irradiation with green light L and the generation of oxygen nanobubble water is performed. Thereby, further improvement in the efficiency of land-based aquaculture of the seawater shrimp A can be achieved.

[0163] <Experiment 4: Domestication efficiency of seawater shrimp A for each breeding water of seawater shrimp A> Below, regarding the details of the relationship between the breeding water 111 and the survival rate of the seawater shrimp A, examples in the case of using the component ratios of the above-described embodiments will be described in comparison with comparative examples.

[0164] In this experimental example, the operation of reducing the salinity concentration of the breeding water 111 to obtain the breeding water 110 was not performed, and the survival rate of the seawater shrimp A before domestication was confirmed. Therefore, although it is described as a "reference example" and a "comparative example", it goes without saying that the same results will be obtained when the salinity concentration is reduced by the salinity reduction step S11.

[0165] In this experiment, regarding the breeding water 111 in which the seawater shrimp A is bred, as the mass of the solute per liter of the breeding water, Mg 2+ , Ca 2+ , K + By comparing the survival rates of the seawater shrimp A in terms of the respective masses and mass ratios, the production efficiency of the seawater shrimp A was confirmed. Note that the survival rate in this example was defined as the survival rate of the seawater shrimp A on the 90th day from the start of breeding of the fry.

[0166] As the breeding water 111, artificial seawater containing, in addition to Mg 2+ , Ca 2+ , K + , about 50 mg / L of SO4 2- , and about 8 mg / L of Sr 2+ (strontium ion) and having a salinity concentration of about 3.2% was used. Note that during the breeding period, no intentional increase or decrease operations were performed on the concentration of SO4 2- , the concentration of Sr 2+ , and the salinity concentration.

[0167] The solute masses in the breeding water 111 compared in this experiment were set as a plurality of combinations selected such that, as the mass of the solute per liter of the breeding water, Mg 2+ was 25 to 350 mg, Ca 2+ was 20 to 320 mg, and K + was 5 to 90 mg. Note that other experimental conditions were the same as in Experiment 1.

[0168] Also, for each of Mg 2+ , Ca 2+ , K + , the first mass ratio (mass of Mg 2+ / mass of K + ), the second mass ratio (mass of Ca 2+ / mass of K+ the mass), and the third mass ratio (Mg 2+ mass / Ca 2+ mass) were calculated. Also, regarding the survival rate of seawater shrimp A, values over 60% were rated as "Evaluation: ○", and values less than 60% were rated as "Evaluation: ×". The combinations and mass ratios of Mg 2+ , Ca 2+ , K + mass corresponding to "Evaluation: ○" were confirmed.

[0169] <Experimental Results on the Survival Rate of Seawater Shrimp A> The results of this experiment are as shown in Table 9.

[0170]

Table 9

[0171] According to Table 9, for the survival rates of banana prawns in each example with "Evaluation: ○", in descending order of survival rate, Reference Example 13 (Mg 2+ : 240 mg / L, Ca 2+ : 180 mg / L, K + : 60 mg / L) was 99%, Reference Example 14 (Mg 2+ : 45 mg / L, Ca 2+ : 60 mg / L, K + : 30 mg / L) was 92%, Reference Example 15 (Mg 2+ : 40 mg / L, Ca 2+ : 60 mg / L, K + : 20 mg / L) was 91%, Reference Example 16 (Mg 2+ : 30 mg / L, Ca 2+ : 30 mg / L, K + : 30 mg / L) was 89%, Reference Example 17 (Mg 2+ : 40 mg / L, Ca 2+ : 30 mg / L, K + : 60 mg / L) was 86%, Reference Example 18 (Mg 2+ : 200 mg / L, Ca 2+ : 200 mg / L, K + : 50 mg / L) was 82%, Reference Example 19 (Mg 2+ : 200 mg / L, Ca 2+ : 120 mg / L, K +: 78% for 40 mg / L), Example 20 (Mg 2+ : 320 mg / L, Ca 2+ : 250 mg / L, K + : 80 mg / L) was 78%, Example 21 (Mg 2+ : 35 mg / L, Ca 2+ : 25 mg / L, K + : 8 mg / L) was 76%, Example 22 (Mg 2+ : 60 mg / L, Ca 2+ : 50 mg / L, K + : 10 mg / L) was 75%. For Examples 13 to 22, since the survival rate was over 60%, it was rated as "Evaluation: ○".

[0172] In Examples 13 to 22, the rearing water 111 contained, as the mass of the solute per liter of the rearing water, 35 to 320 mg of Mg 2+ and 25 to 250 mg of Ca 2+ and 8 to 80 mg of K + and the first mass ratio was 0.7 to 5.0, the second mass ratio was 0.5 to 5.0, and the third mass ratio was 0.7 to 1.4. As a result, the survival rate of the seawater shrimp A could be made 75% or more. Thereby, the production efficiency of the seawater shrimp A can be surely improved.

[0173] In particular, when the third mass ratio was 0.7 to 1.3 (Examples 13 to 20), the survival rate was 78% or more; when the first mass ratio was 0.7 to 4.0 and the second mass ratio was 0.5 to 4.0 (Examples 13 to 18), the survival rate was 82% or more; when the first mass ratio was 1.0 to 4.0 and the second mass ratio was 1.0 to 3.0 (Examples 13 to 16), the survival rate was 89% or more; when the first mass ratio was 1.5 to 4.0 and the second mass ratio was 2.0 to 3.0 (Examples 13 to 15), the survival rate was 91% or more, and it was confirmed that the survival rate was even higher. Thereby, the production efficiency of the seawater shrimp A can be more surely improved.

[0174] Also, the survival rates of the banana prawns in each of the examples rated as "Evaluation: ×", in descending order of the survival rate, were Comparative Example 4 (Mg 2+ : 320 mg / L, Ca 2+ : 250 mg / L, K+ : 52% for Comparative Example 5 (Mg 2+ : 320 mg / L, Ca 2+ : 260 mg / L, K + : 80 mg / L), 52% for Comparative Example 6 (Mg 2+ : 350 mg / L, Ca 2+ : 320 mg / L, K + : 80 mg / L), 48% for Comparative Example 7 (Mg 2+ : 30 mg / L, Ca 2+ : 35 mg / L, K + : 8 mg / L), 32% for Comparative Example 8 (Mg 2+ : 35 mg / L, Ca 2+ : 20 mg / L, K + : 8 mg / L), 30% for Comparative Example 9 (Mg 2+ : 320 mg / L, Ca 2+ : 20 mg / L, K + : 80 mg / L), 12% for Comparative Example 10 (Mg 2+ : 350 mg / L, Ca 2+ : 35 mg / L, K + : 8 mg / L), 8% for Comparative Example 11 (Mg 2+ : 35 mg / L, Ca 2+ : 260 mg / L, K + : 8 mg / L), 8% for Comparative Example 12 (Mg 2+ : 35 mg / L, Ca 2+ : 25 mg / L, K + : 90 mg / L), 5% for Comparative Example 13 (Mg 2+ : 25 mg / L, Ca 2+ : 35 mg / L, K + : 5 mg / L), 5% for Comparative Example 14 (Mg 2+ : 250 mg / L, Ca 2+ : 320 mg / L, K + : 5 mg / L), 4% for Comparative Example 15 (Mg 2+ : 30 mg / L, Ca 2+ : 320 mg / L, K + : 80 mg / L) was 2%.

[0175] In Comparative Example 4, K +Since it exceeds 80 mg / L, the survival rate of marine shrimp A cannot be increased, and a reliable improvement in the production efficiency of marine shrimp A cannot be achieved.

[0176] In Comparative Example 5, since Ca 2+ exceeds 250 mg / L, the survival rate of marine shrimp A cannot be increased, and a reliable improvement in the production efficiency of marine shrimp A cannot be achieved.

[0177] In Comparative Example 6, since Mg 2+ exceeds 320 mg / L, the survival rate of marine shrimp A cannot be increased, and a reliable improvement in the production efficiency of marine shrimp A cannot be achieved.

[0178] In Comparative Example 7, since Mg 2+ is less than 35 mg / L, the survival rate of marine shrimp A cannot be increased, and a reliable improvement in the production efficiency of marine shrimp A cannot be achieved.

[0179] In Comparative Example 8, since Ca 2+ is less than 25 mg / L, the survival rate of marine shrimp A cannot be increased, and a reliable improvement in the production efficiency of marine shrimp A cannot be achieved.

[0180] In Comparative Example 9, since Ca 2+ is less than 25 mg / L, the survival rate of marine shrimp A cannot be increased, and a reliable improvement in the production efficiency of marine shrimp A cannot be achieved.

[0181] In Comparative Example 10, since Mg 2+ exceeds 320 mg / L, the survival rate of marine shrimp A cannot be increased, and a reliable improvement in the production efficiency of marine shrimp A cannot be achieved.

[0182] In Comparative Example 11, since Ca 2+ exceeds 250 mg / L, the survival rate of marine shrimp A cannot be increased, and a reliable improvement in the production efficiency of marine shrimp A cannot be achieved.

[0183] In Comparative Example 12, since K +Since it exceeds 80 mg / L, the survival rate of marine shrimp A cannot be increased, and a reliable improvement in the production efficiency of marine shrimp A cannot be achieved.

[0184] In Comparative Example 13, K + is less than 8 mg / L, so the survival rate of marine shrimp A cannot be increased, and a reliable improvement in the production efficiency of marine shrimp A cannot be achieved.

[0185] In Comparative Example 14, K + is less than 8 mg / L, so the survival rate of marine shrimp A cannot be increased, and a reliable improvement in the production efficiency of marine shrimp A cannot be achieved.

[0186] In Comparative Example 15, Mg 2+ is less than 35 mg / L, so the survival rate of marine shrimp A cannot be increased, and a reliable improvement in the production efficiency of marine shrimp A cannot be achieved.

[0187] That is, the pre-salinity reduction breeding water 111 and the post-salinity reduction breeding water 110 suitable for improving the survival rate of marine shrimp A contain, as the mass of the solute per liter of breeding water, 35 - 320 mg of Mg 2+ and 25 - 250 mg of Ca 2+ and 8 - 80 mg of K + and have a first mass ratio of 0.7 - 5.0, a second mass ratio of 0.5 - 5.0, and a third mass ratio of 0.7 - 1.4. More preferably, the third mass ratio is 0.7 - 1.3, still more preferably, the first mass ratio is 0.7 - 4.0 and the second mass ratio is 0.5 - 4.0, still more preferably, the first mass ratio is 1.0 - 4.0 and the second mass ratio is 1.0 - 3.0, still more preferably, the first mass ratio is 1.5 - 4.0 and the second mass ratio is 2.0 - 3.0.

[0188] Although some embodiments of the present invention have been described, these embodiments are presented by way of example and are not intended to limit the scope of the invention. These novel embodiments can be implemented in various other forms, and various omissions, replacements, and changes can be made without departing from the gist of the invention. These embodiments and their modifications are included in the scope and gist of the invention, and are included in the invention described in the claims and the equivalent scope thereof.

Explanation of Signs

[0189] 1 Acclimation system 11 Rearing tank 110 Rearing water (rearing water with reduced salt concentration) 111 Rearing water (rearing water with a salt concentration at which marine organisms can survive) 112 Rearing water (rearing water with a reduced salt concentration) 12 Salt concentration reduction section 13 Water supply tank 130 Diluted water 14 Waste water tank 140 Waste water 2 Aquaponics 21 Hydroponic cultivation tank 210 Rearing water 22 Decomposition liquid generation section 221 Decomposition liquid 23 Decomposition liquid supply section 31, 31a, 31b Circulation pipe 32 Water supply pipe 33 Drainage pipe 51 Filter device 52 Sterilizing device 53 Bubble generator 54 Water quality monitoring device 55 Temperature control device 56 Automatic feeding device 57 Lighting section A Marine shrimp B Plant S11 Salt concentration reduction process S12 Decomposition liquid generation process S13 Decomposition liquid supply process

Claims

1. The method includes a salinity reduction step for reducing the salinity of the rearing water in which the saltwater shrimp are reared by 0.01% by mass to 0.09% by mass per day, within a range of 0.20% by mass or less. The method for acclimatizing saltwater shrimp is characterized by the above-mentioned.

2. A salinity reduction step of reducing the salinity of the rearing water in which the saltwater shrimp are reared by 0.01% by mass to 0.09% by mass per day within a range of 0.20% by mass or less; A decomposition liquid producing step of biodegrading the excrement of the saltwater shrimp to produce a decomposition liquid; a decomposition liquid supplying step of supplying the decomposition liquid generated by the decomposition liquid generating step to a plant that is hydroponically grown using the breeding water whose salt concentration has been reduced by the salt concentration reducing step; Having An aquaponics cultivation method characterized by the following:

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