Method for increasing the extraction of saccharides and glycoside components and its use

Abstract

To disclose a method for increasing leaching of sugar and / or glycoside components which includes the steps of: (1) preparing a sugar solution and / or a sugar alcohol solution; (2) immersing raw materials in the sugar solution and / or the sugar alcohol solution; and (3), in the sugar solution and / or the sugar alcohol solution, heating the raw materials once to three times for extraction, followed by filtration to obtain an extract.SOLUTION: The aforementioned method can lead to significant improvement in extraction rates of a sugar component and / or a glycoside component from the raw materials, thus contributing to improvements in utilization of the raw materials and their therapeutic effects.SELECTED DRAWING: None

Description

Technical Field

[0001] The present invention relates to the field of chemical component extraction, and specifically to a method for increasing the leaching of saccharide and glycoside components, and its use.

Background Art

[0002] Currently, while ensuring the safety and effectiveness of extracts, how to harmlessly and efficiently extract active ingredients / effective ingredients in raw materials, such as saccharide components and / or glycoside components, is an important issue in the field of researching the extraction of raw materials such as traditional Chinese medicines and botanical medicines. According to modern research, the ethanol extraction efficiency of many components of traditional Chinese medicines is much higher than that of water decoction extraction, but the toxicity of the obtained extracts may increase. In the newly announced registration and declaration of traditional Chinese medicine new drugs in 2020, it is stipulated that if the declared indications are consistent with those in the case of traditional usage, clinical trials can be appropriately reduced or omitted. For example, in 2018, the "Regulations on the Simplified Registration Review and Approval Management of Traditional Chinese Medicine Compound Preparations of Ancient Classical Famous Prescriptions" was announced, clarifying the scope of application where clinical trials can be omitted in the development of traditional Chinese medicine compound preparations of ancient classical famous prescriptions, including that the preparation process is consistent with the descriptions in ancient medical books. Most of the preparation processes of traditional Chinese medicines adopt the water decoction method, and it has been clarified by clinical experience and modern research that this method can further ensure the safety of extracts compared to the organic solvent extraction method. The main reasons for the differences between the two extraction methods are as follows. There are many types of active ingredients contained in traditional Chinese medicines, with a wide polarity range. In ethanol extraction, the extraction rates of weakly polar and moderately polar components are improved, resulting in the composition of the alcohol extract being different from that of the active ingredients in the decoctions traditionally used clinically. Therefore, ethanol extraction may have a certain impact on the safety of extracts. In addition, the water decoction method most commonly used in the traditional extraction of traditional Chinese medicines has problems such as low extraction rate and incomplete extraction. Although an increase in the amount of water used contributes to an improvement in the extraction rate of active ingredients, it leads to an increase in the concentration time and manufacturing cost.

[0003] Therefore, based on conventional methods, how to harmlessly and efficiently improve the extraction rate of active ingredients in raw materials such as extracts of traditional Chinese medicines is an important research direction in the research on the extraction of raw materials such as traditional Chinese medicines.

Summary of the Invention

Problems to be Solved by the Invention

[0004] Based on the above, the present invention provides a method for increasing the leaching of saccharide components and / or glycoside components by using an aqueous solution of a low-concentration saccharide compound, using an aqueous sugar solution and / or a sugar alcohol water of a certain concentration instead of water as an extraction solvent. Compared with the extraction by the conventional decoction method, the extraction rate is greatly improved, and the utilization rate and therapeutic effect of raw materials such as traditional Chinese medicines are enhanced. Since the sugar used in this research is an auxiliary material often used in traditional Chinese medicines or an edible sugar, it has characteristics such as safety and low cost, and will not have a negative impact on the safety of the extract.

Means for Solving the Problems

[0005] Specifically, according to one aspect of the present invention, step (1) of preparing a sugar solution and / or a sugar alcohol solution; step (2) of immersing the raw material in the sugar solution and / or the sugar alcohol solution; step (3) of heating and extracting the raw material 1 to 3 times in the sugar solution and / or the sugar alcohol solution, and filtering to obtain a raw material extract, to provide a method for increasing the leaching of saccharide components and / or glycoside components.

[0006] Furthermore, the concentration of the sugar solution and / or the sugar alcohol solution is 0.1 to 40 g / 100 mL. Furthermore, the concentration of the sugar solution and / or the sugar alcohol solution is 0.1 to 10 g / 100 mL, or 20 to 40 g / 100 mL. Furthermore, the concentration of the sugar solution and / or the sugar alcohol solution is 0.1 to 5 g / 100 mL, or 20 to 30 g / 100 mL. Furthermore, the concentration of the sugar solution and / or the sugar alcohol solution is 0.1 to 2.5 g / 100 mL. Furthermore, the concentration of the sugar solution and / or the sugar alcohol solution is 1 to 2 g / 100 mL. Furthermore, the concentration of the sugar solution and / or the sugar alcohol solution is about 0.5 g / 100 mL, about 1 g / 100 mL, about 1.5 g / 100 mL, about 2 g / 100 mL, about 20 g / 100 mL, about 30 g / 100 mL, or about 40 g / 100 mL.

[0007] Furthermore, the sugar is one or more selected from monosaccharides, disaccharides, and trisaccharides. Furthermore, the sugar alcohol is one or more selected from sorbitol, mannitol, erythritol, maltitol, lactitol, and xylitol. Furthermore, the monosaccharide is one or more selected from glucose, fructose, galactose, mannose, sorbose, rhamnose, ribose, xylose, and deoxyribose. Furthermore, the disaccharide is one or more selected from maltose, sucrose, lactose, trehalose, melibiose, gentiobiose, and kojibiose. Furthermore, the monosaccharide is glucose and / or fructose. Furthermore, the trisaccharide is raffinose. Furthermore, the disaccharide is sucrose and / or maltose.

[0008] Furthermore, the saccharide component is an oligosaccharide component and / or a polysaccharide component.

[0009] Furthermore, the polysaccharide component is one or more selected from astragalus polysaccharide, licorice polysaccharide, ginseng polysaccharide, and wolfberry polysaccharide.

[0010] Furthermore, the glycoside components are one or more selected from flavonoid compounds, saponin compounds, diterpene glycoside compounds, quinone glycoside compounds, and phenolic acid glycoside compounds.

[0011] Furthermore, the flavonoid compounds are flavone glycoside compounds, isoflavone glycoside compounds, dihydroflavone glycoside compounds, dihydroflavonol glycoside compounds, dihydroisoflavonol glycoside compounds, chalcone glycoside compounds, dihydrochalcone glycoside compounds, aurone glycoside compounds, anthocyanin compounds, biflavone glycoside compounds, and flavanol glycoside compounds.

[0012] Furthermore, the saponin compounds are tetracyclic triterpenoid glycoside compounds, pentacyclic triterpenoid glycoside compounds, and / or steroid glycoside compounds.

[0013] Furthermore, the diterpene glycoside compounds are one or more selected from tetracyclic diterpene glycoside compounds, tricyclic diterpene glycoside compounds, chain-like diterpene glycoside compounds, monocyclic diterpene glycoside compounds, and bicyclic diterpene glycoside compounds.

[0014] Furthermore, the quinone glycoside compounds are benzoquinone glycoside compounds, naphthoquinone glycoside compounds, phenanthrenequinone glycoside compounds, and / or anthraquinone glycoside compounds.

[0015] Furthermore, the phenolic acid glycoside compounds are phenolic acid glycoside compounds having a C6-C3 parent nucleus structure and / or phenolic acid glycoside compounds having a C6-C1 parent nucleus structure.

[0016] Furthermore, the steroid glycoside compounds are spirostanol-type saponin compounds, furostanol-type saponin compounds, and / or isospirostanol-type saponin compounds.

[0017] Furthermore, the isospirostanol-type saponin compounds are diosins compounds.

[0018] Furthermore, the furostanol-type saponin compounds are timosaponin compounds.

[0019] Furthermore, the tetracyclic triterpene glycoside compounds are lanostane-type tetracyclic triterpene saponin compounds and / or dammarane-type tetracyclic triterpene saponin compounds.

[0020] Furthermore, the pentacyclic triterpene glycoside compounds are oleanane-type pentacyclic triterpene saponin compounds, ursane-type pentacyclic triterpene saponin compounds and / or lupinan-type pentacyclic triterpene saponin compounds.

[0021] Furthermore, the anthraquinone glycoside compounds are monoanthracene nuclear compounds and / or bisanthracene nuclear compounds.

[0022] Furthermore, the isoflavone glycoside compound is calycosin-7-glucoside and / or ononin.

[0023] Furthermore, the dihydroflavonoid glycoside compound is liquiritin apioside and / or liquiritin. Furthermore, the chalcone glycoside compound is hydroxysafflor yellow A. Furthermore, the furostanol-type saponin compound is timosaponin BII. Furthermore, the lanostane-type tetracyclic triterpenoid saponin compound is astragaloside. Furthermore, the dammarane-type tetracyclic triterpenoid saponin compound is ginsenoside Rg1. Furthermore, the oleanane-type pentacyclic triterpenoid saponin compound is glycyrrhizic acid. Furthermore, the tetracyclic diterpenoid glycoside compounds are stevioside and / or rebaudioside. Furthermore, the phenolic acid glycoside compound having a C6-C3 parent nucleus structure is hydroxysafflor yellow A. Furthermore, the bisanthracene nucleus compounds are sennoside A and / or sennoside B. Furthermore, the monoanthracene nucleus compound is aloin.

[0024] Furthermore, the raw material is a raw material containing a saccharide component and / or a glycoside component.

[0025] Furthermore, the raw material is one or more traditional Chinese medicines, botanical drugs, and / or marine organisms containing a saccharide component and / or a glycoside component.

[0026] Furthermore, the traditional Chinese medicine and / or the botanical drug is one or more selected from ginseng, notoginseng, licorice, wolfberry, stevia, astragalus, senna leaf, albizia bark, pokeweed, bupleurum, pulsatilla, toona sinensis, dendrobium huoshanense, achyranthes bidentata, clematis chinensis, american ginseng, bolbostemma paniculatum, aesculus, acanthopanax senticosus, aesculus wilsonii, gynostemma pentaphyllum, melia azedarach, centella asiatica, actaea rubra, ilicium pubescens, dipsacus asperoides, akebia quinata, japonicus japonicus, lysimachia foenum-graecum, clematis florida, ziziphus jujuba var. spinosa, cannabis, polygala tenuifolia, perilla frutescens var. crispa, eclipta prostrata, platycodon grandiflorum, ophiopogon japonicus, allium macrostemon, polygonatum sibiricum, polygonatum odoratum, anemarrhena asphodeloides, asparagus cochinchinensis, paris vietnamensis, smilax glabra, veratrum nigrum, dioscorea opposita Puncture vine (Tribulus terrestris L.) , solanum violaceum, pomegranate, almond, sweet potato, yam, asparagus, trillium tschonoskii, periploca sepium, taro, and dragon's blood resin.

[0027] Furthermore, in step (2), the immersion is immersion at room temperature.

[0028] Furthermore, the immersion step includes immersing the raw material in the sugar solution and / or the sugar alcohol solution at room temperature for 10 to 120 minutes.

[0029] Furthermore, the immersion step includes immersing the raw material in the sugar solution and / or the sugar alcohol solution at room temperature for 20 to 60 minutes.

[0030] Furthermore, the immersion step includes immersing the raw material in the sugar solution and / or the sugar alcohol solution at room temperature for 30 minutes.

[0031] Furthermore, the weight-to-volume ratio of the raw material to the sugar solution and / or the sugar alcohol solution is 1:1 to 1:50.

[0032] Furthermore, the weight-to-volume ratio of the raw material to the sugar solution and / or the sugar alcohol solution is 1:1 to 1:20.

[0033] Furthermore, the weight-to-volume ratio of the raw material to the sugar solution and / or the sugar alcohol solution is 1:6 to 1:12.

[0034] Furthermore, in step (3), the heating extraction is heating reflux extraction.

[0035] Furthermore, the heating time is 20 minutes to 60 minutes.

[0036] Furthermore, the heating time is 20 minutes to 30 minutes.

[0037] Furthermore, the heating time is about 20 minutes or about 30 minutes.

[0038] According to one aspect of the present invention, there is provided the use of the above method in the preparation of a raw material extract or a raw material concentrate, wherein the raw material is a Chinese herbal medicine, a botanical medicine, and / or a marine organism.

[0039] Furthermore, the concentrate of the raw material is obtained by concentrating all the extract of the raw material obtained in step (3) or step (5) in a water bath at 20°C to 100°C.

[0040] Furthermore, the concentration is carried out in a water bath at 60°C to 80°C.

[0041] Furthermore, the concentration is carried out in a water bath at about 70°C.

[0042] According to one aspect of the present invention, there is provided use of the above method in the preparation of a pharmaceutical composition containing a raw material, wherein the raw material is a traditional Chinese medicine, a botanical drug, and / or a marine organism.

[0043] According to one aspect of the present invention, there is provided use of the above method in the preparation of a pharmaceutical preparation, a functional food, or a health food containing a raw material, wherein the raw material is a traditional Chinese medicine, a botanical drug, and / or a marine organism.

Advantages of the Invention

[0044] The beneficial effects of the present invention are as follows.

[0045] As a result of research, compared with the conventional decoction method, the method of the present invention can effectively improve the extraction rate of saccharide components and / or glycoside components in raw materials such as traditional Chinese medicine or botanical drugs.

Modes for Carrying Out the Invention

[0046] Hereinafter, the technical solutions in the embodiments of the present invention will be clearly and completely described. Of course, the described embodiments are only a part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by those skilled in the art without creative efforts based on the embodiments of the present invention are within the scope of the patent of the present invention.

[0047] Unless there is a contradiction, the embodiments of the present invention and the features of the embodiments may be combined with each other. Hereinafter, the present invention will be described in detail with reference to the embodiments.

[0048] Hereinafter, the present invention will be described in more detail with reference to specific embodiments, but these embodiments should not be understood as limiting the scope claimed by the present invention.

[0049] As described in the background art, compared with the decoctions that have been clinically used conventionally, the composition of the active ingredients in the alcohol extracts is different, so there are concerns about the safety of the alcohol extracts. On the other hand, the efficiency of the conventional extraction by boiling water decoction is insufficient. To solve the above problems, the present invention provides a method for increasing the leaching of saccharide components and / or glycoside components by using sugar. The method includes: Step (1) of preparing a sugar solution and / or a sugar alcohol solution; Step (2) of immersing the raw material in the sugar solution and / or the sugar alcohol solution; Step (3) of heating and extracting the raw material in the sugar solution and / or the sugar alcohol solution, and filtering to obtain a raw material extract.

[0050] The fact that a sugar solution and / or a sugar alcohol solution can improve the extraction rate of saccharide components and / or glycoside components in a raw material, such as traditional Chinese medicine or botanical medicine, is mainly related to the following two factors.

[0051] (1) Whether a liquid can impregnate a solid decoction piece is related to its surface tension. Those with a small surface tension coefficient (about 30×10 -3 N / m) can be impregnated into almost solids. Since water has a large surface tension coefficient, it can only impregnate some solids. Since the surface tension coefficient of the sugar aqueous solution is smaller than that of pure water, the solution can enter the plant cells more easily and release the active ingredients. Therefore, it can promote the impregnation of raw materials, such as traditional Chinese medicine decoction pieces, and promote the elution of saccharide components and / or glycoside components in them.

[0052] (2) A large amount of hydroxy is contained in both the sugar in the extraction solvent and the glycosyl moiety of the saccharide components and / or glycoside components in the extract. Therefore, sugar can form intermolecular forces such as intermolecular hydrogen bonds with the saccharide components and / or glycoside components in a raw material, such as traditional Chinese medicine decoction pieces, thereby improving the extraction efficiency of the saccharide components and / or glycoside components in a raw material, such as traditional Chinese medicine extract.

[0053] Therefore, compared with the decoction method, this method can sufficiently improve the extraction rate of the saccharide components and / or glycoside components of raw materials such as Chinese herbal medicines or botanical medicines.

[0054] When actually implemented, the number of extractions of alcohol and / or sugar alcohol solution may be determined according to actual needs, and it may be once, or multiple times, for example, 2 to 10 times.

[0055] In one preferred embodiment, the concentration of the sugar solution and / or the sugar alcohol solution is 0.1 to 40 g / 100 mL.

[0056] In the present invention, when a concentration, ratio, equivalent, number of times, or other parameter is represented by a range, a preferred range, or a range limited by a series of preferred upper limit values and preferred lower limit values, all ranges formed by any pair of the upper limit or preferred value of any range and the lower limit or preferred value of any range should be understood to be specifically disclosed, even though the ranges are disclosed individually. For example, when a range of "0.1 to 40" is disclosed, unless otherwise specified, this range includes its limit values and all point values within this range, such as 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.0, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3.0, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4.0, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 6.0, 7.0, 8.0, 9.0, 10.0, 11.0, 12.0, 13.0, 14.0, 15.0, 16.0, 17.0, 18.0, 19.0, 20.0, 21.0, 22.0, 23.0, 24.0, 25.0, 26.0, 27.0, 28.0, 29.0, 30.0, 31.0, 32.0, 33.0, 34.0, 35.0, 36.0, 37.0, 38.0, 39.0, 40.0, 0.61, 0.62, 0.63, 0.64, 0.65, 1.21, 1.22, 1.23, 1.24, 1.25, 1.26, 1.27, 1.28, 1.29, etc., but is not limited to these numerical values listed above.

[0057] In one preferred embodiment, the concentration of the sugar solution and / or the sugar alcohol solution is 0.1 to 10 g / 100 mL, or 20 to 40 g / 100 mL.

[0058] In one preferred embodiment, the concentration of the sugar solution and / or the sugar alcohol solution is 0.1 to 5 g / 100 mL, or 20 to 30 g / 100 mL.

[0059] In one preferred embodiment, the concentration of the sugar solution and / or the sugar alcohol solution is 0.1 to 2.5 g / 100 mL.

[0060] In one preferred embodiment, the concentration of the sugar solution and / or the sugar alcohol solution is 0.5 to 2 g / 100 mL.

[0061] In one preferred embodiment, the concentration of the sugar solution and / or the sugar alcohol solution is about 0.5 g / 100 mL, about 1 g / 100 mL, about 1.5 g / 100 mL, about 2 g / 100 mL, about 20 g / 100 mL, about 30 g / 100 mL, or about 40 g / 100 mL.

[0062] The term "about" or "approximately" with respect to a numerical value means ±5% of that numerical value, but explicitly includes the exact numerical value. For example, a concentration of "about" 0.5 g / 100 mL means a concentration of 0.475 g / 100 mL to 0.525 g / 100 mL, but explicitly includes a concentration of 0.5 g / 100 mL; a concentration of "about" 1 g / 100 mL means a concentration of 0.95 g / 100 mL to 1.05 g / 100 mL, but explicitly includes a concentration of 1 g / 100 mL; a concentration of "about" 1.5 g / 100 mL means a concentration of 1.425 g / 100 mL to 1.575 g / 100 mL, but explicitly includes a concentration of 1.5 g / 100 mL; a concentration of "about" 2 g / 100 mL means a concentration of 1.9 g / 100 mL to 2.1 g / 100 mL, but explicitly includes a concentration of 2 g / 100 mL; a concentration of "about" 20 g / 100 mL means a concentration of 19 g / 100 mL to 21 g / 100 mL, but explicitly includes a concentration of 20 g / 100 mL; a concentration of "about" 30 g / 100 mL means a concentration of 28.5 g / 100 mL to 31.5 g / 100 mL, but explicitly includes a concentration of 30 g / 100 mL; a concentration of "about" 40 g / 100 mL means a concentration of 38 g / 100 mL to 42 g / 100 mL, but explicitly includes a concentration of 40 g / 100 mL.

[0063] In one preferred embodiment, the sugar is one or more selected from monosaccharides, disaccharides, and trisaccharides.

[0064] In one preferred embodiment, the sugar alcohol is one or more selected from sorbitol, mannitol, erythritol, maltitol, lactitol, and xylitol. When actually used, the above sugar alcohol may be changed to other sugar alcohols in the prior art according to the actual situation.

[0065] In one preferred embodiment, the monosaccharide is one or more selected from glucose, fructose, galactose, mannose, sorbose, rhamnose, ribose, xylose, and deoxyribose. When actually used, the above monosaccharide may be changed to other monosaccharides in the prior art according to the actual situation.

[0066] In one preferred embodiment, the disaccharide is one or more selected from maltose, sucrose, lactose, trehalose, melibiose, gentiobiose, and kojibiose. When actually used, the above disaccharide may be changed to other disaccharides in the prior art according to the actual situation.

[0067] In one preferred embodiment, the monosaccharide is glucose and / or fructose.

[0068] In one preferred embodiment, the trisaccharide is raffinose.

[0069] In order to further improve the extraction rate of the active ingredient of saponin compounds in raw materials such as traditional Chinese medicines, in one preferred embodiment, the disaccharide is sucrose and / or maltose.

[0070] In one preferred embodiment, the saccharide component is an oligosaccharide component and / or a polysaccharide component.

[0071] In one preferred embodiment, the polysaccharide component is one or more selected from astragalus polysaccharide, licorice polysaccharide, ginseng polysaccharide, and wolfberry polysaccharide.

[0072] In one preferred embodiment, the glycoside components are one or more selected from flavonoid compounds, saponin compounds, diterpenoid glycoside compounds, quinone glycoside compounds, and phenolic acid glycoside compounds.

[0073] In one preferred embodiment, the flavonoid compounds are one or more selected from flavone glycoside compounds, isoflavone glycoside compounds, dihydroflavone glycoside compounds, dihydroflavonol glycoside compounds, dihydroisoflavonol glycoside compounds, chalcone glycoside compounds, dihydrochalcone glycoside compounds, aurone glycoside compounds, anthocyanin compounds, biflavone glycoside compounds, and flavanol glycoside compounds.

[0074] In one preferred embodiment, the saponin compounds are tetracyclic triterpenoid glycoside compounds, pentacyclic triterpenoid glycoside compounds, and / or steroid glycoside compounds.

[0075] In one preferred embodiment, the diterpenoid glycoside compounds are one or more selected from tetracyclic diterpenoid glycoside compounds, tricyclic diterpenoid glycoside compounds, chain diterpenoid glycoside compounds, monocyclic diterpenoid compounds, and bicyclic diterpenoid glycoside compounds.

[0076] In one preferred embodiment, the quinone glycoside compounds are benzoquinone glycoside compounds, naphthoquinone glycoside compounds, phenanthrenequinone glycoside compounds, and / or anthraquinone glycoside compounds.

[0077] In one preferred embodiment, the phenolic acid glycoside compounds are phenolic acid glycoside compounds having a C6-C3 parent nucleus structure and / or phenolic acid glycoside compounds having a C6-C1 parent nucleus structure.

[0078] In one preferred embodiment, the steroid glycoside compound is a spirostanol type saponin compound, a furostanol type saponin compound, and / or an isospirostanol type saponin compound.

[0079] In one preferred embodiment, the isospirostanol type saponin compound is a dioscin compound.

[0080] In one preferred embodiment, the furostanol type saponin compound is a timosaponin compound.

[0081] In one preferred embodiment, the tetracyclic triterpene glycoside compound is a lanostane type tetracyclic triterpene saponin compound, and / or a dammarane type tetracyclic triterpene saponin compound.

[0082] In one preferred embodiment, the pentacyclic triterpene glycoside compound is an oleanane type pentacyclic triterpene saponin compound, an ursane type pentacyclic triterpene saponin compound, and / or a lupinan type pentacyclic triterpene saponin compound.

[0083] In one preferred embodiment, the anthraquinone glycoside compound is a monoanthracene nuclear compound, and / or a bisanthracene nuclear compound.

[0084] In one preferred embodiment, the isoflavone glycoside compound is calycosin-7-glucoside and / or ononin. In one preferred embodiment, the dihydroflavone glycoside compound is liquiritin apioside and / or liquiritin. In one preferred embodiment, the chalcone glycoside compound is hydroxysafflor yellow A. In one preferred embodiment, the furostanol-type saponin compound is timosaponin BII. In one preferred embodiment, the lanostane-type tetracyclic triterpenoid saponin compound is astragaloside. In one preferred embodiment, the dammarane-type tetracyclic triterpenoid saponin compound is ginsenoside Rg1. In one preferred embodiment, the oleanane-type pentacyclic triterpenoid saponin compound is glycyrrhizic acid. In one preferred embodiment, the tetracyclic diterpenoid glycoside compounds are stevioside and / or rebaudioside. In one preferred embodiment, the phenolic acid glycoside compound with a C6-C3 parent nucleus structure is hydroxysafflor yellow A. In one preferred embodiment, the bisanthracene nucleus compounds are sennoside A and / or sennoside B. In one preferred embodiment, the monoanthracene nucleus compound is aloetin.

[0085] In one preferred embodiment, the raw material is a raw material containing a saccharide component and / or a glycoside component.

[0086] In one preferred embodiment, the raw material is one or more traditional Chinese medicines, botanical medicines, and / or marine organisms containing a saccharide component and / or a glycoside component.

[0087] In one preferred embodiment, the Chinese herbal medicine and / or botanical medicine is one or more selected from ginseng, notoginseng, liquorice, wolfberry, stevia, astragalus membranaceus, senna leaf, albizia bark, pokeweed, bupleurum, pulsatilla, toona sinensis, dendrobium huoshanense, achyranthes bidentata, clematis chinensis, American ginseng, bolbostemma paniculatum, horse chestnut, acanthopanax senticosus, aesculus wilsonii, gynostemma pentaphyllum, melia azedarach, centella asiatica, actinidia valvata, ilex pubescens, dipsacus asperoides, akebia quinata, japonicus japonicus, potentilla anserina, cannabis, polygala tenuifolia, perilla frutescens, eclipta prostrata, platycodon grandiflorus, ophiopogon japonicus, allium macrostemon, polygonatum sibiricum, polygonatum odoratum, anemarrhena asphodeloides, asparagus cochinchinensis, ophiopogon japonicus, paris vietnamensis, smilax glabra, veratrum nigrum, yam Puncture vine (Tribulus terrestris L.) and one or more selected from solanum violaceum, pomegranate, almond, sweet potato, yam, asparagus, trillium tschonoskii, cortex periplocae, taro, and dragon's blood resin.

[0088] In one preferred embodiment, in step (2), the immersion is an immersion at room temperature.

[0089] In one preferred embodiment, the immersion step includes immersing the raw material in the sugar solution and / or the sugar-alcohol solution at room temperature for 10 to 120 minutes.

[0090] In one preferred embodiment, the immersion step includes immersing the raw material in the sugar solution and / or the sugar-alcohol solution at room temperature for 20 to 60 minutes.

[0091] In one preferred embodiment, the immersion step includes immersing the raw material in the sugar solution and / or the sugar-alcohol solution at room temperature for about 30 minutes.

[0092] The term "about" or "approximately" regarding a numerical value means ±5% of the said numerical value, but explicitly includes the explicit numerical value. For example, the time of "about" 30 minutes is the time from 28.5 minutes to 31.5 minutes, but explicitly includes the time of 30 minutes.

[0093] In one preferred embodiment, the weight-to-volume ratio of the raw material to the sugar solution and / or the sugar-alcohol solution is 1:1 to 1:50.

[0094] In one preferred embodiment, the weight-to-volume ratio of the raw material to the sugar solution and / or the sugar-alcohol solution is 1:1 to 1:20.

[0095] In one preferred embodiment, the weight - volume ratio of the raw material to the sugar solution and / or the sugar - alcohol solution is 1:6 to 1:12.

[0096] In one preferred embodiment, in step (3), the heat extraction is heat reflux extraction.

[0097] In one preferred embodiment, the heating time is 20 minutes to 60 minutes.

[0098] In one preferred embodiment, the heating time is 20 minutes to 30 minutes.

[0099] In one preferred embodiment, the heating time is about 20 minutes or about 30 minutes.

[0100] The term "about" or "approximately" regarding a numerical value means ±5% of the said numerical value, but explicitly includes the exact numerical value. For example, the time of "about" 30 minutes means the time from 28.5 minutes to 31.5 minutes, but explicitly includes 30 minutes; the time of "about" 20 minutes means the time from 19 minutes to 21 minutes, but explicitly includes 20 minutes.

[0101] According to one aspect of the present invention, there is provided the use of the above - mentioned method in the preparation of a raw material extract or a raw material concentrate, wherein the raw material is a Chinese herbal medicine, a botanical medicine, and / or a marine organism.

[0102] In one preferred embodiment, the raw material concentrate is obtained by concentrating all the extract of the raw material obtained in step (3) of the above - mentioned steps or the extract of the raw material obtained in step (5) in a water bath at 20°C to 100°C.

[0103] In one preferred embodiment, the concentration is carried out in a water bath at 60°C to 80°C.

[0104] In one preferred embodiment, the concentration is carried out in a water bath at about 70°C.

[0105] The term "about" or "around" regarding a numerical value means ±5% of the said numerical value, but explicitly includes the exact numerical value. For example, a temperature of "about" 70°C means a range of 66.5°C to 73.5°C, but explicitly includes a temperature of 70°C.

[0106] According to one aspect of the present invention, there is provided the use of the above method in the preparation of a pharmaceutical composition containing a raw material, wherein the raw material is a traditional Chinese medicine, a botanical medicine, and / or a marine organism.

[0107] According to one aspect of the present invention, there is provided the use of the above method in the manufacture of a pharmaceutical preparation, a functional food or a health food containing a raw material, wherein the raw material is a traditional Chinese medicine, a botanical medicine, and / or a marine organism.

[0108] Among these, a functional food is a food that has been convincingly proven to be beneficial to one or more functions of the body and has a sufficient nutritional effect to improve the health status or reduce diseases.

[0109] A health food refers to a food that has a specific health maintenance function or is intended for supplementing vitamins and minerals, that is, a food suitable for a specific person to eat, that regulates the body's functions, is not intended for the treatment of diseases, and does not cause any acute, subacute or chronic harm to the human body.

[0110] According to one aspect of the present invention, there is provided the use of the above method in the manufacture of other products containing a raw material, wherein the other products are all products included in the prior art containing raw materials such as traditional Chinese medicines, botanical medicines and / or biological preparations, excluding pharmaceutical preparations, functional foods and health foods, and including products in forms such as liquid and solid.

[0111] It should be noted that the examples and features of the present application may be combined with each other without contradiction. Hereinafter, the present invention will be described in detail with reference to the examples.

[0112] Hereinafter, the present invention will be described in more detail with reference to specific embodiments, which should not be construed as limiting the scope claimed in the present application.

[0113] Examples

[0114] Apparatus and Reagents Agilent 1260 High Performance Liquid Chromatography equipped with a DAD detector (Agilent Technologies, USA); Agilent 1200 High Performance Liquid Chromatography equipped with an ELSD detector (Agilent Technologies, USA); UV-2550 Ultraviolet-Visible Spectrophotometer (Shimadzu Corporation); DZKW-4 Electronic Constant Temperature Water Bath (Beijing Zhongxing Weiye Instrument Co., Ltd.); DK-98-IIA Electric Constant Temperature Water Bath Pot (Tianjin Test Instrument Co., Ltd.); ME204 / 02 Electronic Analytical Balance (Mettler-Toledo Instruments (Shanghai) Co., Ltd., high-precision balance); KQ-250DE CNC Ultrasonic Cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); HC-3018 High-Speed Centrifuge (Anhui Zhongke Zhongjia Scientific Instrument Co., Ltd.); Solid Phase Extraction Cartridge UniEIut C18 EC Column (1000 mg / 6 ml) (Huapu Keyi Beijing Technology Co., Ltd.).

[0115] As controls, liquiritin apioside (lot number CHB180109), liquiritin (lot number CHB201102), astragaloside (lot number CHB170727), calycosin-7-glucoside (lot number CHB161105), ononin (lot number CHB150517), D-glucose (lot number CHB190213) (purchased from Chengdu Cromar Biotechnology Co., Ltd., purity > 98%), glycyrrhizic acid (lot number Z30A6B1) (purchased from Shanghai Yuanye Biotechnology Co., Ltd., purity > 98%), ginsenoside Rg1 (lot number 210708) (purchased from Chengdu Plant Standardized Pure Biotechnology Co., Ltd., purity > 98%), timosaponin BII (lot number 111839-202107) (purchased from the National Institutes for Food and Drug Control, purity ≥ 95.4%), rebaudioside (lot number 211117), stevioside (lot number 211024) (purchased from Chengdu Plant Standardized Pure Biotechnology Co., Ltd., purity > 98%), sennoside A (lot number 211203) (purchased from Chengdu Plant Standardized Pure Biotechnology Co., Ltd., purity by HPLC > 98%), sennoside B (lot number 220215) (purchased from Chengdu Plant Standardized Pure Biotechnology Co., Ltd., purity by HPLC > 98%), acetonitrile (chromatographically pure, Fisher), methanol (chromatographically pure, Fisher), phosphoric acid (analytically pure, purchased from Sinopharm Chemical Reagent Co., Ltd.), sucrose, glucose, fructose, maltose, lactose (BioClone (Beijing) Biotechnology Co., Ltd.), trehalose (Hebei Baiwei Biotechnology Co., Ltd.). All of the above are of pharmaceutical grade. The water is purified water produced by Wahaha Group.

[0116] Example 1 Extraction experiment of raw licorice slices using each sugar solution 1.1 Preparation method of licorice extract with each sugar Four aliquots of 40 g each of the same lot of raw licorice slices (produced in Inner Mongolia) were prepared. 7 times the amount of water (280 mL of water), 1% glucose aqueous solution (2.8 g of glucose + 280 mL of water), 1% sucrose aqueous solution (2.8 g of sucrose + 280 mL of water), and 1% maltose aqueous solution (2.8 g of maltose + 280 mL of water) were added to each, immersed for 30 minutes, refluxed for 30 minutes, and filtered through gauze while it was hot to obtain the licorice extract with each sugar.

[0117] 1.2 Measurement of the Contents of Liquiritin Apioside, Liquiritin, and Glycyrrhizic Acid (HPLC Pharmacy Method) 1.2.1 Preparation of the Test Article Solution To 0.5 mL of the licorice extract with each sugar, 9.5 mL of methanol was added and diluted 20-fold, shaken uniformly, and filtered through a 0.22 μm filter membrane to obtain the test article solution.

[0118] 1.2.2 Preparation of the Reference Standard Solution Stock solution: Appropriate amounts of the reference standards of liquiritin apioside, liquiritin, and glycyrrhizic acid were accurately weighed, methanol was added, and a stock solution of 1 mg / mL was prepared. It was stored at -20 °C in a refrigerator.

[0119] Linear working solution: Appropriate amounts of the liquiritin apioside and liquiritin stock solutions were diluted by adding methanol to obtain a series of mixed standard linear working solutions of 2.5, 5, 10, 20, 40, 50, 100 μg / mL. Appropriate amounts of the glycyrrhizic acid stock solution were diluted by adding methanol to obtain a series of mixed standard linear working solutions of 10, 20, 40, 80, 160, 200, 400 μg / mL.

[0120] 1.2.3 Measurement Method for the Content Column for chromatography: Thermo Acclaim 120 C18 (250×4.6 mm, 5 μm); Mobile phase: Acetonitrile (A), 0.05% phosphoric acid solution (B); Column temperature: 35 °C, Detection wavelength: 237 nm, Injection volume: 10 μL, Flow rate: 1 ml / min, Gradient elution: 0 - 8 minutes, 19% A; 8 - 35 minutes, 19% - 50% A; 35 - 36 minutes, 50% - 100% A; 36 - 40 minutes, 100% - 19% A; 40 - 50 minutes, 19% A.

[0121] 1.3 Experimental Results The measurement results of the contents of the active ingredients in the licorice extract with each sugar are shown in Table 1.

[0122]

Table 1

[0123] Example 2 Extraction experiment of raw licorice slices using each sugar solution 2.1 Method for preparing licorice extract with each sugar Four aliquots of 40 g each of the same lot of raw licorice slices (produced in Xinjiang) were prepared. 7 times the amount of water (280 mL of water), 1% sucrose aqueous solution (2.8 g of sucrose + 280 mL of water), 1% maltose aqueous solution (2.8 g of maltose + 280 mL of water), and 1% lactose aqueous solution (2.8 g of lactose + 280 mL of water) were sequentially added, immersed for 30 minutes, refluxed for 30 minutes, filtered through gauze while hot, and 6 times the amount of water, 1% sucrose aqueous solution (2.4 g of sucrose + 240 mL of water), 1% maltose aqueous solution (2.4 g of maltose + 240 mL of water), and 1% lactose aqueous solution (2.4 g of lactose + 240 mL of water) were added to the medicinal residues respectively, refluxed for 20 minutes, filtered while hot, and the two filtrates were combined to obtain licorice extracts with each sugar.

[0124] 2.2 Measurement of liquiritin apioside, liquiritin, and glycyrrhizic acid contents (HPLC pharmacy method) 2.2.1 Preparation of test article solution It was the same as the method for preparing the test article solution in Example 1.

[0125] 2.2.2 Preparation of reference article solution It was the same as the method for preparing the reference article solution in Example 1.

[0126] 2.2.3 Method for measuring content It was the same as the method for measuring content in Example 1.

[0127] 2.3 Experimental results The measurement results of the content of active ingredients in the licorice extracts with each sugar are shown in Table 2.

[0128]

Table 2

[0129] As can be seen by combining the results of Table 1 and Table 2, sucrose can increase the extraction rates of the main active ingredients in licorice, namely flavonoids (liquiritin apioside, liquiritin) and saponin components (glycyrrhizic acid).

[0130] Example 3 Extraction experiment of Astragalus membranaceus slices using each sugar solution 3.1 Method for preparing Astragalus membranaceus extract with each sugar (extracting twice for each 1% sugar) Five aliquots of 50 g each of the same lot of Astragalus membranaceus slices (obtained from Hunyuan, Shanxi Province) were prepared. To each, 7 times the amount of water (350 ml of water), 1% glucose aqueous solution (add water to 3.5 g of glucose and make up to 350 ml), 1% fructose aqueous solution (add water to 3.5 g of fructose and make up to 350 ml), 1% sucrose aqueous solution (add water to 3.5 g of sucrose and make up to 350 ml), 1% trehalose aqueous solution (add water to 3.5 g of trehalose and make up to 350 ml) were added, soaked for 30 minutes, refluxed for 30 minutes, filtered through gauze while hot, and further, 6 times the amount of water (300 ml of water), 1% glucose aqueous solution (add water to 3.0 g of glucose and make up to 300 ml), 1% fructose aqueous solution (add water to 3.0 g of fructose and make up to 300 ml), 1% sucrose aqueous solution (add water to 3.0 g of sucrose and make up to 300 ml), 1% trehalose aqueous solution (add water to 3.0 g of trehalose and make up to 300 ml) were added to the medicinal residues respectively, refluxed for 20 minutes, filtered while hot, and the two filtrates were combined to obtain the Astragalus membranaceus extract with each sugar.

[0131] 3.2 Measurement of the contents of astragaloside, calycosin-7-glucoside, and ononin 3.2.1 Preparation of Test Article Solution Preparation of Test Article Solution Containing Saponin Component: To 5 ml of the Astragalus extract with each sugar, add 20 ml of 60% methanol, mix by ultrasonic wave, add 10 ml of ammonia water, mix uniformly, centrifuge at 3600 r / min for 10 minutes, pass the supernatant through a UniEIut C18 EC column (1000 mg / 6 ml) manufactured by Chromatography Society, elute with 10 ml of purified water, then elute with 5 ml of methanol, collect the methanol eluate, centrifuge at 8000 r / min for 5 minutes, pass the supernatant through a 0.45 μm microporous filter membrane to obtain the test article solution.

[0132] Preparation of Test Article Solution Containing Flavonoid Component: To 300 μl of the Astragalus extract with each sugar, add 700 μl of water, place it in a 1.5 ml centrifuge tube, centrifuge at 12000 r / min for 5 minutes, pass the supernatant through a 0.45 μm microporous filter membrane to obtain the test article solution containing flavonoid component.

[0133] 3.2.2 Preparation of Control Article Solution Accurately weigh appropriate amounts of astragaloside, calycosin-7-glucoside, and ononin reference substances, add methanol to prepare a stock solution of 1 mg / ml. Dilute the stock solution to different consecutive concentrations to prepare a working curve.

[0134] The linear equation of astragaloside is

[0135]

Number

[0136]

Number

[0137]

Number

[0138] 3.2.3 Measurement method of content Measurement method of saponin component content: Analyzed using an Agilent 1200 series liquid chromatograph (equipped with an ELSD detector). Chromatographic column: Thermo Acclaim 120 C18 (250×4.6 mm, 5 μm); Mobile phase: 0.1% formic acid - water (A) ~ 0.1% formic acid - acetonitrile (B), gradient elution: 0 - 5 minutes, 5% - 10% B; 5 - 10 minutes, 10% - 32% B; 10 - 30 minutes, 32% - 45% B; 30 - 35 minutes, 45% - 95% B; 35 - 40 minutes, 95% - 20% B); Column temperature: room temperature, flow rate 1 ml / min; Drift tube temperature: 100 °C; Carrier gas flow rate: 2.5 l / min, injection volume: 20 μl.

[0139] Measurement method of flavonoid component content: Analyzed using an Agilent 1260 series high - performance liquid chromatograph (equipped with a DAD detector). Chromatographic column: Thermo Acclaim 120 C18 (250×4.6 mm, 5 μm); Mobile phase: 0.1% formic acid - aqueous solution (A) and 0.1% formic acid - acetonitrile (B), gradient elution: 0 - 8 minutes, 5% - 20% B; 8 - 15 minutes, 20% - 25% B; 15 - 20 minutes, 25% B; 20 - 30 minutes, 25% - 40% B; 30 - 40 minutes, 40% - 60% B; Flow rate: 1 ml / min; Detection wavelength: 260 nm; Column temperature: 25 °C; Injection volume: 10 μl.

[0140] 3.3 Experimental results The measurement results of the content of active ingredients in the Astragalus extract with each sugar are shown in Table 3.

[0141]

Table 3

[0142] Example 4 Extraction experiment of raw Astragalus membranaceus tablets using each sugar solution 4.1 Method for preparing Astragalus membranaceus extract with each sugar (extraction once for each 1% sugar) Four aliquots of 50 g each of the same lot of raw Astragalus membranaceus tablets (obtained from Hunyuan, Shanxi Province) were prepared. To each, 7 times the amount of water (350 ml of water), 1% glucose aqueous solution (add water to 3.5 g of glucose and make up to 350 ml), 1% sucrose aqueous solution (add water to 3.5 g of sucrose and make up to 350 ml), and 1% maltose aqueous solution (add water to 3.5 g of maltose and make up to 350 ml) were added. They were soaked for 30 minutes, refluxed for 30 minutes, and filtered through gauze while hot to obtain Astragalus membranaceus extracts with each sugar.

[0143] 4.2 Determination of the contents of astragalosides, calycosin-7-glucoside, and ononin 4.2.1 Preparation of test article solutions It was the same as the method for preparing test article solutions in Example 3.

[0144] 4.2.2 Preparation of reference article solutions It was the same as the method for preparing reference article solutions in Example 3.

[0145] 4.2.3 Method for content determination It was the same as the method for content determination in Example 3.

[0146] 4.3 Experimental results The measurement results of the contents of active ingredients in Astragalus membranaceus extracts with each sugar are shown in Table 4.

[0147]

Table 4

[0148] Example 5 Extraction experiment of raw Astragalus membranaceus tablets using each sugar solution 5.1 Method for preparing Astragalus membranaceus extract with each sugar (extraction twice for each 1% sugar) Four aliquots of 50 g each of the same lot of raw Astragalus membranaceus tablets (obtained from Lingchuan, Shanxi) were prepared. To each, 7 times the amount of water (350 ml of water), 1% sucrose aqueous solution (adding water to 3.5 g of sucrose and making up to 350 ml), 1% maltose aqueous solution (adding water to 3.5 g of maltose and making up to 350 ml), and 1% lactose aqueous solution (adding water to 3.5 g of lactose and making up to 350 ml) were added, immersed for 30 minutes, refluxed for 30 minutes, filtered through gauze while hot, and then to the medicinal residues, 6 times the amount of water (300 ml of water), 1% sucrose aqueous solution (adding water to 3.0 g of sucrose and making up to 300 ml), 1% maltose aqueous solution (adding water to 3.0 g of maltose and making up to 300 ml), and 1% lactose aqueous solution (adding water to 3.0 g of lactose and making up to 300 ml) were added respectively, refluxed for 20 minutes, filtered while hot, and the two filtrates were combined to obtain the Astragalus membranaceus extract with each sugar.

[0149] 5.2 Determination of the contents of astragaloside, calycosin - 7 - glucoside, and ononin 5.2.1 Preparation of test article solution It was the same as the method for preparing the test article solution in Example 3.

[0150] 5.2.2 Preparation of reference article solution It was the same as the method for preparing the reference article solution in Example 3.

[0151] 5.2.3 Method for content determination It was the same as the method for content determination in Example 3.

[0152] 5.3 Experimental results Table 5 shows the measurement results of the content of active ingredients in the sugar-astragalus extract.

[0153]

Table 5

[0154] As can be seen from the combined results of Tables 3 to 5, as a major active ingredient in astragalus, sucrose can significantly improve the extraction rates of flavonoid (calycosin-7-glucoside, ononin) and saponin components (astragaloside).

[0155] Example 6 Extraction of raw astragalus tablets using sucrose solutions of various concentrations 6.1 Preparation of sucrose-astragalus extracts of various concentrations (extraction with sucrose at each concentration from 0 to 10%) Fifty grams of raw Astragalus membranaceus slices (obtained from Lingchuan, Shanxi) were taken for each of the seven aliquots. Seven-fold volume of water (350 ml of water), 0.5% sucrose aqueous solution (add water to 1.8 g of sucrose and make up to 350 ml), 1% sucrose aqueous solution (add water to 3.5 g of sucrose and make up to 350 ml), 1.25% sucrose aqueous solution (add water to 4.4 g of sucrose and make up to 350 ml), 1.5% sucrose aqueous solution (add water to 5.3 g of sucrose and make up to 350 ml), 2% sucrose aqueous solution (add water to 7 g of sucrose and make up to 350 ml), 10% sucrose aqueous solution (add water to 35 g of sucrose and make up to 350 ml) were added to each aliquot, soaked for 30 minutes, refluxed for 30 minutes, filtered through gauze while it was hot, and further, six-fold volume of water (300 ml of water), 0.5% sucrose aqueous solution (add water to 1.5 g of sucrose and make up to 300 ml), 1% sucrose aqueous solution (add water to 3.0 g of sucrose and make up to 300 ml), 1.25% sucrose aqueous solution (add water to 3.8 g of sucrose and make up to 300 ml), 1.5% sucrose aqueous solution (add water to 4.5 g of sucrose and make up to 300 ml), 2% sucrose aqueous solution (add water to 6.0 g of sucrose and make up to 300 ml), 10% sucrose aqueous solution (add water to 30 g of sucrose and make up to 300 ml) were added to the medicinal residues respectively, refluxed for 20 minutes, filtered while it was hot, and the two filtrates were combined to obtain sucrose-Astragalus membranaceus extracts of each concentration.

[0156] 6.2 Determination of the Contents of Astragaloside, Calycosin-7-glucoside, and Ononin 6.2.1 Preparation of Test Article Solutions It was the same as the method for preparing the test article solutions in Example 3.

[0157] 6.2.2 Preparation of Control Article Solutions It was the same as the method for preparing the control article solutions in Example 3.

[0158] 6.2.3 Method for Determining the Contents It was the same as the method for determining the contents in Example 3.

[0159] 6.3 Experimental Results Table 6 shows the measurement results of the content of active ingredients in the Astragalus extract obtained by extracting Astragalus slices with sucrose solutions of different concentrations.

[0160]

Table 6

[0161] Example 7 Extraction experiment of ginsenoside Rg1 in Panax notoginseng using sugar solutions of different concentrations 7.1 Preparation method of sucrose-Panax notoginseng extracts of different concentrations Four aliquots of 50 g each of the same lot of raw Panax notoginseng slices (origin: Yunnan; manufacturer: Tianjin Shengshi Pharmaceutical Co., Ltd.; lot number: X20102703A) were prepared. To each, 7 times the amount of water (350 ml of water), 0.5% sucrose aqueous solution (add water to 1.75 g of sucrose and make up to 350 ml), 1% sucrose aqueous solution (add water to 3.5 g of sucrose and make up to 350 ml), 1.5% sucrose aqueous solution (add water to 5.25 g of sucrose and make up to 350 ml) were added, soaked for 30 minutes, refluxed for 30 minutes, filtered through gauze while hot, and then to the medicinal residues, 6 times the amount of water (300 ml of water), 0.5% sucrose aqueous solution (add water to 1.5 g of sucrose and make up to 300 ml), 1% sucrose aqueous solution (add water to 3.0 g of sucrose and make up to 300 ml), 1.5% sucrose aqueous solution (add water to 4.5 g of sucrose and make up to 300 ml) were added respectively, refluxed for 20 minutes, filtered while hot, and the two filtrates were combined to obtain sucrose-Panax notoginseng extracts of different concentrations.

[0162] 7.2 Measurement of the content of ginsenoside Rg1 7.2.1 Preparation of the test article solution Precisely weigh 2 mL of each notoginseng extract, add methanol to each, make up the volume to 10 mL in a volumetric flask, mix uniformly, and centrifuge at 13000 r·min -1 for 5 minutes. Pass the supernatant through a 0.22-μm microporous filter membrane to obtain the test article solution.

[0163] 7.2.2 Preparation of reference substance solution Take an appropriate amount of the reference substance of ginsenoside Rg1, precisely weigh it, add methanol to prepare a stock solution of 1 mg / ml. Dilute the stock solution to different consecutive concentrations to prepare a working curve.

[0164] The linear equation of ginsenoside Rg1 is

[0165]

Number

[0166] 7.2.3 Measurement method of content Analysis was performed using an Agilent 1200 liquid chromatograph. Chromatographic column: Roc C18 (250×4.6 mm, 5 μm); Mobile phase: water (A) - acetonitrile (B), gradient elution: 0 - 12 minutes, 19% → 19%B; 12 - 60 minutes, 19% → 36%B; Column temperature: 35°C; Flow rate: 1 ml / min; Injection volume: 10 μl; Detection wavelength: 203 nm.

[0167] 7.3 Experimental results Table 7 shows the effects of sucrose at various concentrations on the extraction rate of marker components in notoginseng.

[0168]

Table 7

[0169] Example 8 Extraction experiment of timosaponin BII in Anemarrhenae Rhizoma slices using sucrose solutions of various concentrations 8.1 Preparation method of sucrose - Anemarrhenae Rhizoma extracts of various concentrations Five aliquots of 50 g each of the same batch of raw Anemarrhenae Rhizoma slices were prepared. To each, 7 times the amount of water (350 ml of water), 0.5% sucrose aqueous solution (dissolve 1.75 g of sucrose in water and make up to 350 ml), 1% sucrose aqueous solution (dissolve 3.5 g of sucrose in water and make up to 350 ml), 1.5% sucrose aqueous solution (dissolve 5.25 g of sucrose in water and make up to 350 ml), 2.0% sucrose aqueous solution (dissolve 7.0 g of sucrose in water and make up to 350 ml) were added, soaked for 30 minutes, refluxed for 30 minutes, filtered through gauze while hot, and then to the medicinal residues, 6 times the amount of water (300 ml of water), 0.5% sucrose aqueous solution (dissolve 1.5 g of sucrose in water and make up to 300 ml), 1% sucrose aqueous solution (dissolve 3.0 g of sucrose in water and make up to 300 ml), 1.5% sucrose aqueous solution (dissolve 4.5 g of sucrose in water and make up to 300 ml), 2.0% sucrose aqueous solution (dissolve 6 g of sucrose in water and make up to 300 ml) were added respectively, refluxed for 20 minutes, filtered while hot, the two filtrates were combined and made up to 500 mL to obtain sucrose - Anemarrhenae Rhizoma extracts of various concentrations.

[0170] 8.2 Determination of the content of timosaponin BII 8.2.1 Preparation of the test article solution Precisely weigh 2 mL of each Anemarrhenae Rhizoma extract, add methanol to each and make up to 10 mL in a volumetric flask, mix uniformly, and centrifuge at 13000 r·min -1 for 5 minutes, and pass the supernatant through a 0.22 μm microporous filter membrane to obtain the test article solution.

[0171] 8.2.2 Preparation of the reference article solution An appropriate amount of the reference substance of timosaponin BII was accurately weighed, and 30% acetonitrile was added to prepare a stock solution with a mass concentration of 1 mg / ml. The stock solution was diluted to different consecutive concentrations to prepare a working curve.

[0172] The linear equation of timosaponin BII is

[0173]

Number

[0174] 8.2.3 Measurement method of content Analysis was carried out using an Agilent 1200 type liquid chromatograph. Chromatographic column: Inertsil ODS-3-C18 (250×4.6 mm, 5 μm); Mobile phase: water (A) - acetonitrile (B), isocratic elution with 25% acetonitrile; Column temperature: 35 °C; Flow rate: 0.8 ml / min; Injection volume: 10 μl. Drift tube temperature: 104 °C; Carrier gas flow rate: 2.8 L·min -1 .

[0175] 8.3 Experimental results Table 8 shows the influence of sucrose at each concentration on the extraction rate of the marker components in Anemarrhena asphodeloides.

[0176]

Table 8

[0177] Example 9 Extraction experiment of sennoside B and sennoside A in Senna leaves using sucrose solutions at each concentration 9.1 Preparation method of sucrose-Senna leaf extracts at each concentration Twenty grams of senna leaves from the same lot (origin: Yulin, Guangxi; lot number: 200101) were prepared into eight aliquots. To each aliquot, 12 times the amount of water (240 ml of water), 0.5% sucrose aqueous solution (prepared by dissolving 1.2 g of sucrose in 240 ml of water), 0.75% sucrose aqueous solution (prepared by dissolving 1.8 g of sucrose in 240 ml of water), 1% sucrose aqueous solution (prepared by dissolving 2.4 g of sucrose in 240 ml of water), 1.5% sucrose aqueous solution (prepared by dissolving 3.6 g of sucrose in 240 ml of water), 2% sucrose aqueous solution (prepared by dissolving 4.8 g of sucrose in 240 ml of water), 3% sucrose aqueous solution (prepared by dissolving 7.2 g of sucrose in 240 ml of water), 5% sucrose aqueous solution (prepared by dissolving 12 g of sucrose in 240 ml of water) were added. The mixture was immersed for 30 minutes, refluxed for 30 minutes, filtered through three layers of gauze while it was hot. Further, to the medicinal residues, 10 times the amount of water (200 ml of water), 0.5% sucrose aqueous solution (prepared by dissolving 1 g of sucrose in 200 ml of water), 0.75% sucrose aqueous solution (prepared by dissolving 1.5 g of sucrose in 200 ml of water), 1% sucrose aqueous solution (prepared by dissolving 2 g of sucrose in 200 ml of water), 1.5% sucrose aqueous solution (prepared by dissolving 3 g of sucrose in 200 ml of water), 2% sucrose aqueous solution (prepared by dissolving 4 g of sucrose in 200 ml of water), 3% sucrose aqueous solution (prepared by dissolving 6 g of sucrose in 200 ml of water), 5% sucrose aqueous solution (prepared by dissolving 10 g of sucrose in 200 ml of water) were added respectively. The mixture was refluxed for 20 minutes and filtered through three layers of gauze while it was hot. The two filtrates were combined and made up to a constant volume of 400 mL to obtain sucrose-senna leaf extracts of each concentration.

[0178] 9.2 Determination of the contents of sennoside B and sennoside A 9.2.1 Preparation of the test article solution Precisely weigh 1 mL of the senna leaf extract of each concentration, make up to 5 ml in a volumetric flask, dilute the sample 5-fold, mix uniformly, and centrifuge at 15000 r·min -1 for 5 minutes. Pass the supernatant through a 0.22 μm microporous filter membrane to obtain the test article solution.

[0179] 9.2.2 Preparation of the reference article solution Take appropriate amounts of the reference substances of sennoside B and sennoside A, add 0.1% NaHCO3, and prepare a stock solution with a mass concentration of 1 mg / ml. Next, dilute it with methanol to reference stock solutions of 0.2 mg / ml, 0.1 mg / ml, 0.05 mg / ml, 0.025 mg / ml, and 0.0125 mg / ml, and shake evenly to obtain the reference solution. The linear equation of sennoside B is

[0180]

Number

[0181]

Number

[0182] 9.2.3 Measurement method of content Analysis was carried out using an Agilent 1260 liquid chromatograph (equipped with an ultraviolet detector).

[0183] Chromatographic column: C18 (250 mm × 4.6 mm, 5 μm); Injection volume: 10 μl; Detection wavelength: 340 nm; Flow rate: 1 ml / min; Column temperature: 30 °C; Mobile phase A: 0.1% phosphoric acid - water, Mobile phase B: acetonitrile; Gradient elution (0 - 10 minutes, 79% A; 10 - 15 minutes, 79% → 75% A; 15 - 16 minutes, 75% A; 16 - 17 minutes, 75% → 79% A).

[0184] 9.3 Experimental results Table 9 shows the effects of sucrose at each concentration on the extraction rate of marker components in senna leaves.

[0185]

Table 9

[0186] Example 10 Extraction experiment of aloin in aloe using sucrose solutions of various concentrations and sugar solutions of the same concentration 10.1.1 Preparation method of sucrose-aloe extracts of various concentrations Six aliquots of 20 g each of the same lot of aloe (purchased from Beijing Tongrentang) were prepared. To each, 12 times the amount of water (240 ml of water), 0.5% sucrose aqueous solution (prepared by dissolving 1.2 g of sucrose in 240 ml of water), 1% sucrose aqueous solution (prepared by dissolving 2.4 g of sucrose in 240 ml of water), 1.5% sucrose aqueous solution (prepared by dissolving 3.6 g of sucrose in 240 ml of water), 2% sucrose aqueous solution (prepared by dissolving 4.8 g of sucrose in 240 ml of water), and 3% sucrose aqueous solution (prepared by dissolving 7.2 g of sucrose in 240 ml of water) were added. After soaking for 30 minutes and refluxing for 30 minutes, it was filtered through one layer of terylene while it was hot and made up to 240 mL to obtain sucrose-aloe extracts.

[0187] 10.1.2 Preparation method of aloe extracts with various sugars Four aliquots of 20 g each of the same lot of aloe (purchased from Beijing Tongrentang) were prepared. To each, 12 times the amount of water (240 ml of water), 0.5% sucrose aqueous solution (prepared by dissolving 1.2 g of sucrose in 240 ml of water), 0.5% maltose aqueous solution (prepared by dissolving 1.2 g of maltose in 240 ml of water), and 0.5% trehalose aqueous solution (prepared by dissolving 1.2 g of trehalose in 240 ml of water) were added. After soaking for 30 minutes and refluxing for 30 minutes, it was filtered through one layer of terylene while it was hot and made up to 240 mL to obtain sugar-aloe extracts.

[0188] 10.2 Measurement of aloin content 10.2.1 Preparation of test article solutions Precisely weigh 0.2 mL of aloe extracts of various concentrations, make up to 10 ml in a volumetric flask, dilute the sample 50 times, mix uniformly, and centrifuge at 15000 r·min-1 Centrifuge for 5 minutes and pass the supernatant through a 0.22 μm microporous filter membrane to obtain the test article solution.

[0189] 10.2.2 Preparation of control article solution Take an appropriate amount of the control article of aloenin, add methanol to prepare a stock solution with a mass concentration of 1 mg / ml, and then dilute it with methanol to control article stock solutions of 0.2 mg / ml, 0.1 mg / ml, 0.05 mg / ml, 0.3 mg / ml, 0.15 mg / ml, and 0.075 mg / ml. Shake evenly to obtain the control article solution.

[0190] The linear equation of aloenin is

[0191]

Number

[0192] 10.2.3 Measurement method of content Analysis was performed using an Agilent 1260 liquid chromatograph (equipped with an ultraviolet detector).

[0193] Column for chromatography: C18 (250 mm × 4.6 mm, 5 μm); injection volume: 5 μl; detection wavelength: 355 nm; flow rate: 1 ml / min; mobile phase A: water, B phase: acetonitrile, isocratic elution (0 - 15 minutes, 75% A).

[0194] 10.3 Experimental results Table 10 shows the effects of sucrose at various concentrations on the extraction rate of marker components in aloe.

[0195]

Table 10

[0196]

Table 11

[0197] Example 11 Extraction experiment of stevioside and rebaudioside in Stevia rebaudiana using sucrose solutions of each concentration and sugar solutions of the same concentration 11.1 Preparation method of sucrose-Stevia rebaudiana extract of each concentration Five aliquots of 20 g each of the same lot of Stevia rebaudiana were prepared. To each, 12 times the amount of water (240 ml of water), 0.5% sucrose aqueous solution (prepared by dissolving 1.2 g of sucrose in 240 ml of water), 1% sucrose aqueous solution (prepared by dissolving 2.4 g of sucrose in 240 ml of water), 1.5% sucrose aqueous solution (prepared by dissolving 3.6 g of sucrose in 240 ml of water), 2% sucrose aqueous solution (prepared by dissolving 4.8 g of sucrose in 240 ml of water) were added, immersed for 30 minutes, refluxed for 30 minutes, filtered through three layers of gauze while hot, and further, 10 times the amount of water (200 ml of water), 0.5% (prepared by dissolving 1 g of sucrose in 200 ml of water), 1% sucrose aqueous solution (prepared by dissolving 2 g of sucrose in 200 ml of water), 1.5% sucrose aqueous solution (prepared by dissolving 3 g of sucrose in 200 ml of water), 2% (prepared by dissolving 4 g of sucrose in 200 ml of water) were added to the crude drug respectively, refluxed for 20 minutes, filtered through three layers of gauze while hot, the two filtrates were combined and made up to 400 mL to obtain sucrose-Stevia rebaudiana extracts of each concentration.

[0198] 11.2 Preparation method of Stevia rebaudiana extract with each sugar Twenty grams of stevia from the same lot were prepared into five aliquots. To each aliquot, 12 times the amount of water (240 ml of water), 1.5% sucrose aqueous solution (prepared by dissolving 3.6 g of sucrose in 240 ml of water), 1.5% maltose aqueous solution (prepared by dissolving 3.6 g of maltose in 240 ml of water), 1.5% trehalose aqueous solution (prepared by dissolving 3.6 g of trehalose in 240 ml of water), and 1.5% lactose aqueous solution (prepared by dissolving 3.6 g of lactose in 240 ml of water) were added. The mixture was immersed for 30 minutes and refluxed for 30 minutes, then filtered through three layers of gauze while it was still hot. Furthermore, 10 times the amount of water (200 ml of water), 1.5% sucrose aqueous solution (prepared by dissolving 3 g of sucrose in 200 ml of water), 1.5% maltose aqueous solution (prepared by dissolving 3 g of maltose in 200 ml of water), 1.5% trehalose aqueous solution (prepared by dissolving 3 g of trehalose in 200 ml of water), and 1.5% lactose aqueous solution (prepared by dissolving 3 g of lactose in 200 ml of water) were added to the crude drug respectively. The mixture was refluxed for 20 minutes and filtered through three layers of gauze while it was still hot. The two filtrates were combined and made up to a constant volume of 400 mL to obtain stevia extracts with each sugar at the same concentration.

[0199] 11.3 Determination of the content of marker components 11.3.1 Preparation of test article solution Precisely weigh 0.5 mL of the stevia extract at each concentration, add methanol and make up to a constant volume of 10 ml, dilute the sample 20-fold, mix uniformly, and centrifuge at 14000 r·min -1 for 5 minutes. Pass the supernatant through a 0.22 μm microporous filter membrane to obtain the test article solution.

[0200] 11.3.2 Preparation of reference article solution Rebaudioside: Take an appropriate amount of the reference article, add methanol to prepare a stock solution with a mass concentration of 1 mg / ml. Then, dilute it with methanol to reference article stock solutions of 0.4 mg / ml, 0.3 mg / ml, 0.2 mg / ml, 0.15 mg / ml, 0.1 mg / ml, and 0.05 mg / ml, shake uniformly to obtain the reference article solution.

[0201] Stevioside: An appropriate amount of the reference substance was taken, methanol was added to prepare a stock solution with a mass concentration of 1 mg / ml. Next, it was diluted with methanol to reference substance stock solutions with concentrations of 0.3 mg / ml, 0.2 mg / ml, 0.15 mg / ml, 0.1 mg / ml, 0.05 mg / ml, and 0.025 mg / ml, and shaken uniformly to obtain the reference substance solutions.

[0202] The linear equation of rebaudioside is

[0203]

Number

[0204]

Number

[0205] 11.3.3 Measurement method of content Analysis was carried out using an Agilent 1260 liquid chromatograph (equipped with an ultraviolet detector).

[0206] Chromatography column: C18 (250 mm × 4.6 mm, 5 μm), ultraviolet detector; injection volume: 5 μl; detection wavelength: 210 nm; flow rate: 0.6 ml / min; column temperature: 35 °C; mobile phase: 0.1% phosphoric acid - water A - acetonitrile B. Isocratic elution of the mobile phase with 30% acetonitrile for 25 minutes.

[0207] 11.4 Experimental results Table 12 shows the influence of sucrose at each concentration on the extraction rate of marker components in Stevia rebaudiana.

[0208]

Table 12

[0209] Table 13 shows the effects of various disaccharides on the extraction rate of marker components in Stevia rebaudiana Bertoni.

[0210]

Table 13

[0211] Example 12 Extraction experiment of hydroxysafflor yellow A in Carthamus tinctorius L. using sucrose solutions of various concentrations and sugar solutions of the same concentration 12.1 Preparation method of sucrose-Carthamus tinctorius L. extracts of various concentrations Twenty grams each of the same batch of Coptis chinensis Franch. (Tibet, Beijing Tongrentang Co., Ltd.) were used to prepare seven aliquots. To each aliquot, 12 times the amount of water (240 ml of water), 0.5% sucrose aqueous solution (prepared by dissolving 1.2 g of sucrose in 240 ml of water), 1% sucrose aqueous solution (prepared by dissolving 2.4 g of sucrose in 240 ml of water), 1.5% sucrose aqueous solution (prepared by dissolving 3.6 g of sucrose in 240 ml of water), 2% sucrose aqueous solution (prepared by dissolving 4.8 g of sucrose in 240 ml of water), 2.5% sucrose aqueous solution (prepared by dissolving 6 g of sucrose in 240 ml of water), 5% sucrose aqueous solution (prepared by dissolving 12 g of sucrose in 240 ml of water) were added, and the mixture was immersed for 30 minutes, refluxed for 30 minutes, filtered through three layers of gauze while it was hot, and further, to the medicinal residues, 10 times the amount of water (200 ml of water), 0.5% sucrose aqueous solution (prepared by dissolving 1 g of sucrose in 200 ml of water), 1% sucrose aqueous solution (prepared by dissolving 2 g of sucrose in 200 ml of water), 1.5% sucrose aqueous solution (prepared by dissolving 3 g of sucrose in 200 ml of water), 2% sucrose aqueous solution (prepared by dissolving 4 g of sucrose in 200 ml of water), 2.5% sucrose aqueous solution (prepared by dissolving 5 g of sucrose in 200 ml of water), 5% sucrose aqueous solution (prepared by dissolving 10 g of sucrose in 200 ml of water) were added respectively, refluxed for 20 minutes, filtered through three layers of gauze while it was hot, the two filtrates were combined, and the volume was fixed to 400 mL to obtain the extract.

[0212] 12.2 Preparation method of Coptis chinensis Franch. extract with each sugar Twenty grams each of the same batch of Coptis chinensis Franch. (from Tibet, Beijing Tongrentang Co., Ltd.) were taken to prepare three aliquots. To each aliquot, 12 times the amount of water (240 ml of water), 0.5% sucrose aqueous solution (prepared by dissolving 1.2 g of sucrose in 240 ml of water), and 0.5% maltose aqueous solution (prepared by dissolving 1.2 g of maltose in 240 ml of water) were added, and the mixture was immersed for 30 minutes, refluxed for 30 minutes, filtered through three layers of gauze while it was hot, and further, 10 times the amount of water (200 ml of water), 0.5% sucrose aqueous solution (prepared by dissolving 1 g of sucrose in 200 ml of water), and 0.5% maltose aqueous solution (prepared by dissolving 1 g of maltose in 200 ml of water) were added to the medicinal residues respectively, refluxed for 20 minutes, filtered through three layers of gauze while it was hot, the two filtrates were combined, and the volume was fixed to 400 mL to obtain the extract.

[0213] 12.3 Determination of the content of marker components 12.3.1 Preparation of the test article solution Precisely weigh 1 mL of the extract of Coptis chinensis Franch. at each concentration, add 1 ml of methanol, dilute the sample by 2 times, mix uniformly, and centrifuge at 15000 r·min -1 for 5 minutes, and pass the supernatant through a 0.22 μm microporous filter membrane to obtain the test article solution.

[0214] 12.3.2 Preparation of the reference article solution Take an appropriate amount of the reference substance of hydroxysafflor yellow A, add methanol to prepare a stock solution with a mass concentration of 1 mg / ml, and then dilute it with methanol to reference stock solutions of 0.2 mg / ml, 0.1 mg / ml, 0.05 mg / ml, 0.025 mg / ml, and 0.0125 mg / ml, and shake uniformly to obtain the reference article solution.

[0215] The linear equation of hydroxysafflor yellow A is

[0216]

Number

[0217] 12.3.3 Method for determining the content Analysis was performed using an Agilent 1260 liquid chromatograph (equipped with an ultraviolet detector). Chromatography column: C18 (250 mm × 4.6 mm, 5 μm); injection volume: 5 μl; detection wavelength: 403 nm; flow rate: 1 ml / min; mobile phase A: 0.5% phosphoric acid - water, B phase: acetonitrile, gradient elution (0 - 10 minutes, 5% → 11% B; 10 - 16 minutes, 11% → 14% B; 16 - 23 minutes, 14% B; 23 - 30 minutes, 14% → 20% B; 30 - 60 minutes, 20% → 31% B; 30 - 60 minutes, 20% → 31% B; 60 - 65 minutes, 31% → 90% B; 65 - 67 minutes, 90% → 5% B).

[0218] 12.4 Experimental results Table 14 shows the influence of sucrose at each concentration on the extraction rate of marker components in Astragalus membranaceus.

[0219]

Table 14

[0220] Table 15 shows the influence of various disaccharides on the extraction rate of marker components in Astragalus membranaceus.

[0221]

Table 15

[0222] Example 13 Extraction experiment of Astragalus polysaccharide with each sugar 13.1 Preparation method of Astragalus membranaceus extract with each sugar 50 g of Astragalus membranaceus slices from the same lot were prepared into five aliquots. To each aliquot, 7-fold volume of water (350 ml of water), 1% sucrose aqueous solution (add water to 3.5 g of sucrose and make up to 350 ml), 1% glucose aqueous solution (add water to 3.5 g of glucose and make up to 350 ml), 1% fructose aqueous solution (add water to 3.5 g of fructose and make up to 350 ml), 1% trehalose aqueous solution (add water to 3.5 g of trehalose and make up to 350 ml), 1% lactose aqueous solution (add water to 3.5 g of lactose and make up to 350 ml), 1% maltose aqueous solution (add water to 3.5 g of maltose and make up to 350 ml) were added, soaked for 30 minutes, refluxed for 30 minutes, filtered with gauze while it was hot, and further to the medicinal residues, 6-fold volume of water (300 ml of water), 1% sucrose aqueous solution (add water to 3.0 g of sucrose and make up to 300 ml), 1% glucose aqueous solution (add water to 3.0 g of glucose and make up to 300 ml), 1% fructose aqueous solution (add water to 3.0 g of fructose and make up to 300 ml), 1% trehalose aqueous solution (add water to 3.0 g of trehalose and make up to 300 ml), 1% lactose aqueous solution (add water to 3.0 g of lactose and make up to 300 ml), 1% maltose aqueous solution (add water to 3 g of maltose and make up to 300 ml) were added respectively, refluxed for 20 minutes, filtered while it was hot, and the two filtrates were combined to obtain the Astragalus membranaceus extract with each sugar.

[0223] 13.2 Measurement of total polysaccharide content 13.2.1 Preparation method of test article solution Precisely weigh 1 ml of the aqueous extract of Astragalus membranaceus, put it into a 15 mL centrifuge tube, add 4 mL of absolute ethanol, mix uniformly, soak for 12 h, and centrifuge at 7000 r·min -1 for 20 minutes, precipitate and discard the supernatant, dissolve the precipitate in water, make up to the mark in a 25 ml volumetric flask, shake uniformly to obtain the test article solution.

[0224] 13.2.2 Preparation method of reference article solution Take an appropriate amount of anhydrous glucose reference substance, precisely weigh it, add water to make a solution containing 0.132 mg of anhydrous glucose per 1 ml to obtain the reference article solution.

[0225] 13.2.3 Selection of Measurement Wavelength Precisely weigh 0.6 ml of the test article solution, place it in a graduated test tube with a stopper of 10 ml, add water to each up to 2.0 ml, shake uniformly, accurately add 1 ml of 5% phenol solution to each, vortex mix for 20 s, add 5 ml of concentrated sulfuric acid and vortex mix for 20 s. After incubating in a water bath at 80°C for 20 minutes and taking it out immediately, cool it in an ice-water bath for 10 minutes, take it out, and perform a scan within the wavelength range of 400 - 800 nm. Since there is a maximum absorption peak at 490 nm, it was determined to measure the total polysaccharide content in the astragalus water extract using 490 nm.

[0226] 13.2.4 Preparation of Standard Curve Precisely weigh 0.1 ml, 0.2 ml, 0.4 ml, 0.6 ml, 0.8 ml, and 1 ml of the reference substance solution, place each in a graduated test tube with a stopper of 10 ml, add water to each up to 2.0 ml, shake uniformly, accurately add 1 ml of 5% phenol solution to each, vortex mix for 20 s, add 5 ml of concentrated sulfuric acid and vortex mix for 20 s. After incubating in a water bath at 80°C for 20 minutes and taking it out immediately, cool it in an ice-water bath for 10 minutes, take it out, and use the corresponding reagent as the blank. According to the ultraviolet-visible spectrophotometry (General Rule 0401), measure the absorbance at a wavelength of 490 nm. Using the absorbance as the vertical coordinate and the concentration as the horizontal coordinate, prepare a standard curve and obtain a linear equation.

[0227] 13.3 Experimental Results The measurement results of the total polysaccharide content in the astragalus extract by each sugar are shown in Table 16.

[0228]

Table 16

[0229] Example 14 Extraction Experiment of Sucrose-Astragalus Polysaccharide at Each Concentration 14.1 Preparation Method of Sucrose-Astragalus Extract at Each Concentration Seven 50-g aliquots of the same batch of raw Astragalus membranaceus slices were prepared. To each aliquot, 7 times the amount of water (350 ml of water), 1% sucrose aqueous solution (add water to 3.5 g of sucrose and make up to 350 ml), 2% sucrose aqueous solution (add water to 7 g of sucrose and make up to 350 ml), 10% sucrose aqueous solution (add water to 35 g of sucrose and make up to 350 ml), 20% sucrose aqueous solution (add water to 70 g of sucrose and make up to 350 ml), 30% sucrose aqueous solution (add water to 105 g of sucrose and make up to 350 ml), and 40% sucrose aqueous solution (add water to 140 g of sucrose and make up to 350 ml) were added. After soaking for 30 minutes, refluxing for 30 minutes, filtering with gauze while it was hot, and then adding 6 times the amount of water (300 ml of water), 1% sucrose aqueous solution (add water to 3.0 g of sucrose and make up to 300 ml), 2% sucrose aqueous solution (add water to 6.0 g of sucrose and make up to 300 ml), 10% sucrose aqueous solution (add water to 30 g of sucrose and make up to 300 ml), 20% sucrose aqueous solution (add water to 60 g of sucrose and make up to 300 ml), 30% sucrose aqueous solution (add water to 90 g of sucrose and make up to 300 ml), and 40% sucrose aqueous solution (add water to 120 g of sucrose and make up to 300 ml) to the medicinal residues respectively, refluxing for 20 minutes, filtering while it was hot, combining the two filtrates, and obtaining the sucrose-Astragalus extracts at each concentration.

[0230] 14.2 Measurement of Total Polysaccharide Content It was the same as in Example 13.

[0231] 14.3 Experimental Results The measurement results of the total polysaccharide content in the Astragalus extracts obtained with sucrose at each concentration are shown in Table 17.

[0232]

Table 17

[0233] Example 15 Extraction experiments of sucrose - glycyrrhiza polysaccharide at each concentration 15.1 Preparation method of sucrose - glycyrrhiza extracts at each concentration Seven aliquots of 50 g each of the same batch of raw licorice slices (produced in Xinjiang) were prepared. To each, 7 times the amount of water (350 ml of water), 1% sucrose aqueous solution (3.5 g of sucrose + 350 ml of water), 2% sucrose aqueous solution (7.0 g of sucrose + 350 ml of water), 10% sucrose aqueous solution (35 g of sucrose + 350 ml of water), 20% sucrose aqueous solution (70 g of sucrose + 350 ml of water), 30% sucrose aqueous solution (105 g of sucrose + 350 ml of water), 40% sucrose aqueous solution (140 g of sucrose + 350 ml of water) were added, immersed for 30 minutes, refluxed for 30 minutes, filtered through gauze while it was hot, and then to the medicinal residues, 6 times the amount of water (300 ml of water), 1% sucrose aqueous solution (3.0 g of sucrose + 300 ml of water), 2% sucrose aqueous solution (6.0 g of sucrose + 300 ml of water), 10% sucrose aqueous solution (30 g of sucrose + 300 ml of water), 20% sucrose aqueous solution (60 g of sucrose + 300 ml of water), 30% sucrose aqueous solution (90 g of sucrose + 300 ml of water), 40% sucrose aqueous solution (120 g of sucrose + 300 ml of water) were added respectively, refluxed for 20 minutes, filtered while it was hot, and the two filtrates were combined to obtain the extract.

[0234] 15.2 Measurement of total polysaccharide content 15.2.1 Preparation of test article solution Precisely weigh 2 ml of the water extract of licorice, add 8 ml of ethanol, mix uniformly, immerse for 12 h, and centrifuge at 5000 r·min -1 for 20 minutes to precipitate, discard the supernatant, dissolve the precipitate in water, make up the volume to 50 ml in a volumetric flask, shake uniformly to obtain the test article solution.

[0235] 15.2.2 Preparation of Reference Solution An appropriate amount of anhydrous glucose reference was accurately weighed, water was added, and a solution (10 ml) containing 0.34 mg of anhydrous glucose per 1 ml was obtained to get the reference solution.

[0236] 15.2.3 Selection of Measurement Wavelength 1.0 ml of the test article solution was accurately weighed, placed in a graduated test tube with a 10 ml stopper, 2.0 ml of water was added to each, shaken uniformly, 8 ml of 0.2% anthrone-sulfuric acid solution was slowly added dropwise, mixed uniformly, placed in a boiling water bath and incubated for 10 minutes. Immediately after taking out, it was placed in an ice water bath and cooled for 10 minutes, then taken out and scanned within the wavelength range of 400 - 800 nm. Since there was a maximum absorption peak at 593 nm, it was determined to measure the total polysaccharide content in the glycyrrhiza water extract using 593 nm.

[0237] 15.2.4 Preparation of Standard Curve 0.1 ml, 0.2 ml, 0.4 ml, 0.6 ml, 0.8 ml, 1 ml of the reference solution were accurately weighed, placed in graduated test tubes with 10 ml stoppers respectively, 2.0 ml of water was added to each, shaken uniformly, 8 ml of 0.2% anthrone-sulfuric acid solution was slowly added dropwise, mixed uniformly, placed in a boiling water bath and incubated for 10 minutes. Immediately after taking out, it was placed in an ice water bath and cooled for 10 minutes, then taken out, and the corresponding reagent was used as the blank. According to the ultraviolet-visible spectrophotometry (General Rule 0401), the absorbance was measured at a wavelength of 593 nm. With the absorbance as the vertical coordinate and the concentration as the horizontal coordinate, a standard curve was prepared to obtain a linear equation.

[0238] 15.3 Experimental Results The measurement results of the total polysaccharide content in the glycyrrhiza water extract by each sugar are shown in Table 18.

[0239]

Table 18

[0240] Example 16 Extraction experiment of glycyrrhiza polysaccharide with each sugar 16.1 Method for preparing glycyrrhiza extract with each sugar Three aliquots of 50 g each of the same lot of raw licorice slices (produced in Xinjiang) were prepared. 7 times the amount of water (350 ml of water), 20% maltose aqueous solution (70 g of maltose + 350 ml of water), and 20% sucrose aqueous solution (70 g of lactose + 350 ml of water) were added to each, immersed for 30 minutes, refluxed for 30 minutes, filtered through gauze while it was hot, and then 6 times the amount of water (300 ml of water), 20% maltose aqueous solution (60 g of maltose + 300 ml of water), and 20% sucrose aqueous solution (60 g of lactose + 300 ml of water) were added to the medicinal residues respectively, refluxed for 20 minutes, filtered while it was hot, and the two filtrates were combined to obtain the extract.

[0241] 16.2 Measurement of total polysaccharide content It was the same as in Example 15.

[0242] 16.3 Experimental results The measurement results of the total polysaccharide content in the glycyrrhiza water extract with each sugar are shown in Table 19.

[0243]

Table 19

[0244] Example 17 Extraction experiment of sucrose - ginseng polysaccharide with each concentration 17.1 Method for preparing sucrose - ginseng extract with each concentration Forty grams of ginseng slices from the same lot (produced in Fusong, Jilin) were prepared into six aliquots. Seven times the amount of water, 1%, 10%, 20%, 25%, and 30% sucrose aqueous solutions were added to each, immersed for 30 minutes, refluxed for 30 minutes, filtered through gauze while hot, and further, six times the amount of water, 1%, 10%, 20%, 25%, and 30% sucrose aqueous solutions were added to the medicinal residues, refluxed for 20 minutes, and filtered through two layers of gauze while hot. The two filtrates were combined, water was added to make a constant volume of 800 mL, and an extract was obtained.

[0245] 17.2 Measurement of Total Polysaccharide Content 17.2.1 Preparation Method of Test Article Solution Precisely weigh 1 mL of the ginseng extract with sucrose at each concentration, add 4 mL of absolute ethanol to each, mix uniformly, immerse for 12 h, and centrifuge at 7000 r·min -1 for 20 minutes, precipitate, discard the supernatant, dissolve the precipitate in water, make a constant volume in a 10 mL volumetric flask, shake uniformly, and obtain the test article solution.

[0246] 17.2.2 Preparation Method of Reference Article Solution Take an appropriate amount of anhydrous glucose reference article, precisely weigh it, add water to make a solution containing 0.132 mg of anhydrous glucose per 1 mL, and obtain the reference article solution.

[0247] 17.2.3 Measurement of Total Polysaccharide Content Precisely weigh 0.4, 0.4, 0.3, 0.3, 0.3, and 0.2 mL of the test article solution of the ginseng extract with sucrose at each concentration, put them into 10 mL stoppered graduated test tubes, add water to 2.0 mL each, shake uniformly, accurately add 1 mL of 5% phenol solution to each, vortex mix for 20 s, add 5 mL of concentrated sulfuric acid and vortex mix for 20 s, incubate in a water bath at 80 °C for 20 minutes, then immediately take it out and cool in an ice water bath for 10 minutes, using the corresponding reagent (water) as the blank. Measure the absorbance at a wavelength of 489 nm.

[0248] 17.2.4 Preparation of Standard Curve Precisely weigh 0.1 mL, 0.2 mL, 0.4 mL, 0.6 mL, 0.8 mL, and 1 mL of the reference solution, place each into a graduated test tube with a 10 mL stopper, add water to each up to 2.0 mL, shake uniformly, accurately add 1 mL of 5% phenol solution to each, vortex mix for 20 s, add 5 mL of concentrated sulfuric acid and vortex mix for 20 s, incubate in a water bath at 80 °C for 20 minutes, then immediately take out and cool in an ice water bath for 10 minutes, take out, and use the corresponding reagent as a blank. According to the ultraviolet-visible spectrophotometry (General Rule 0401), measure the absorbance at a wavelength of 489 nm. Create a standard curve with the absorbance as the vertical axis and the concentration as the horizontal axis.

[0249] 17.3 Experimental Results The influence of sucrose at each concentration on the extraction rate of ginseng polysaccharide is shown in Table 20.

[0250]

Table 20

[0251] Example 18 Extraction Experiment of Ginseng Polysaccharide with Each Sugar 18.1 Preparation Method of Various Disaccharide-Ginseng Extracts Prepare 40 g each of the same batch of ginseng tablets (produced in Fusong, Jilin) into 3 aliquots. Add 7 times the amount of water, 30% sucrose, and 30% maltose aqueous solutions to each, soak for 30 minutes, and perform the following operations in the same manner as in Example 17.

[0252] 18.2 Measurement of Total Polysaccharide Content It was the same as in Example 17.

[0253] 18.3 Experimental Results The influence of various disaccharides on the extraction rate of total ginseng polysaccharide is shown in Table 21.

[0254]

Table 21

[0255] Example 19 Extraction experiment of sucrose - wolfberry polysaccharide at each concentration 19.1 Method for preparing wolfberry extract Six aliquots of 40 g each of the same batch of wolfberries (produced in Ningxia) were prepared. To each, 7 times the amount of water (280 ml of water), 1% sucrose aqueous solution (2.8 g of sucrose + 280 m of water), 10% sucrose aqueous solution (28 g of sucrose + 280 m of water), 20% sucrose aqueous solution (56 g of sucrose + 280 m of water), 30% sucrose aqueous solution (84 g of sucrose + 280 m of water), 40% sucrose aqueous solution (112 g of sucrose + 280 m of water), and 30% maltose aqueous solution (84 g of maltose + 280 m of water) were added, immersed for 30 minutes, refluxed for 30 minutes, filtered through gauze while it was hot, and then 6 times the amount of water, 1% sucrose aqueous solution, 10% sucrose aqueous solution, 20% sucrose aqueous solution, 30% sucrose aqueous solution, 40% sucrose aqueous solution, and 30% maltose aqueous solution were added to the medicinal residues respectively, refluxed for 20 minutes, and filtered through two - layer gauze while it was hot. The two filtrates were combined and water was added to make the volume constant to 800 mL.

[0256] 19.2 Measurement of total polysaccharide content 19.2.1 Method for preparing the test article solution It was the same as the method for preparing the test article solution in Example 17.

[0257] 19.2.2 Preparation of the reference article solution It was the same as the method for preparing the reference article solution in Example 17.

[0258] 19.2.3 Measurement of total polysaccharide content Precisely weigh 0.4, 0.4, 0.4, 0.3, and 0.3 mL of the wolfberry extract test solutions of sucrose at each concentration, place them in graduated test tubes with stoppers of 10 mL, add water to each up to 2.0 mL, shake well uniformly, accurately add 1 mL of 5% phenol solution to each, vortex mix for 20 s, add 5 mL of concentrated sulfuric acid and vortex mix for 20 s, incubate in a water bath at 80 °C for 20 minutes, then immediately take out and cool in an ice water bath for 10 minutes, using the corresponding reagent (water) as the blank. Measure the absorbance at a wavelength of 489 nm.

[0259] 19.2.4 Preparation of Standard Curve It was the same as the method for preparing the standard curve in Example 17.

[0260] 19.3 Experimental Results Table 22 shows the influence of sucrose at each concentration on the extraction rate of wolfberry polysaccharide.

[0261]

Table 22

[0262] Example 20 Extraction Experiment of Wolfberry Polysaccharide with Each Sugar 20.1 Preparation Method of Various Disaccharides - Wolfberry Extract Prepare 40 g each of the same batch of wolfberry tablets (production area: Ningxia; manufacturer: Tianjin Shengshi Pharmaceutical Co., Ltd.; lot number: X20122702A) into 3 aliquots. Add 7 times the amount of water, 30% sucrose, and 30% maltose aqueous solution to each, immerse for 30 minutes, and perform the following operations in the same manner as in Example 19.

[0263] 20.2 Measurement of Total Polysaccharide Content It was the same as in Example 19.

[0264] 20.3 Experimental Results Table 23 shows the effects of various disaccharides on the extraction rate of wolfberry polysaccharides.

[0265]

Table 23

[0266] The above are only examples of the present invention, and common knowledge such as specific structures and characteristics known in this specification is not described in detail here. For those skilled in the art, without departing from the present invention, it is also possible to make some modifications and improvements, which should also be regarded as within the protection scope of the present invention, and none of these will affect the implementation effect of the present invention and the practicality of the patent. The protection scope claimed by the present invention shall be based on the content of its claims, and the specific embodiments described in the specification are those that can be used to interpret the claims.

[0267] The embodiments of the present invention have been described in detail above. In this specification, specific examples have been applied to explain the principle and embodiments of the present invention, and the description of the above embodiments is only used to help understand the method of the present invention and its core concept. Also, any changes or modifications made by those skilled in the art based on the idea of the present invention based on the specific embodiments and application scope of the present invention all belong to the protection scope of the present invention. As described above, the content of this specification should not be understood as limiting the present invention.

Claims

1. Step (1) of preparing a sucrose solution of 0.95 to 1.05 g / 100 mL or 10 to 30 g / 100 mL; Step (2) of immersing the raw material in the sucrose solution; Step (3) of heating and extracting the raw material 1 to 3 times in the sucrose solution, filtering to obtain a raw material extract, which is a method for increasing the leaching of saccharide components and / or glycoside components, wherein the concentration of the sucrose solution for increasing the leaching of the saccharide components is 10 to 30 g / 100 mL, and the saccharide components are astragalus polysaccharide derived from astragalus, glycyrrhiza polysaccharide derived from licorice, ginseng polysaccharide derived from ginseng or wolfberry polysaccharide derived from wolfberry; the concentration of the sucrose solution for increasing the leaching of the glycoside components is 0.95 to 1.05 g / 100 mL, and the glycoside components are calycosin-7-glucoside derived from astragalus, ononin derived from astragalus, astragaloside derived from astragalus, liquiritin apioside derived from licorice, liquiritin derived from licorice, glycyrrhizic acid derived from licorice, ginsenoside Rg1 derived from notoginseng, timosaponin BII derived from anemarrhena, rebaudioside derived from stevia or hydroxysafflower yellow A derived from carthamus tinctorius, Method.

2. In step (2), the immersion is immersion at room temperature, the method according to claim 1.

3. In step (2), the immersion step includes immersing the raw material in the 0.95 to 1.05 g / 100 mL or 10 to 30 g / 100 mL sucrose solution at room temperature for 10 to 120 minutes, the method according to claim 2.

4. In step (2), the weight-to-volume ratio of the raw material to the 0.95 to 1.05 g / 100 mL or 10 to 30 g / 100 mL sucrose solution is 1:1 to 1:50, the method according to claim 2.

5. In step (3), the heating extraction is heating under reflux extraction, the method according to claim 1.

6. In step (3), the heating time is 20 minutes to 60 minutes, the method according to claim 5.

7. Use of the method according to any one of claims 1 to 6 in the preparation of a pharmaceutical composition containing a raw material extract or a raw material concentrate or a raw material, or in the manufacture of a pharmaceutical preparation, a functional food or a health food containing the raw material, wherein the raw material is astragalus, licorice, ginseng, wolfberry, notoginseng, anemarrhena, stevia or carthamus tinctorius, Use.

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