Peptides and their uses

Abstract

The purpose of the present invention is to provide a novel peptide, a novel use of the peptide and a method for producing the peptide.　An intestinal barrier function-improving agent, a blood sugar elevation-inhibiting agent, an insulin sensitivity-improving agent, an FGF21 secretion promoter, a stress suppressant or a neuroprotectant, or a fatigue-reducing agent containing a peptide that comprises any one of amino acid sequences LIVTQTMKGL (SEQ ID NO: 1), LIVTQTMKG (SEQ ID NO: 2), LIVTQTMK (SEQ ID NO: 3), IVTQTMKGL (SEQ ID NO: 4), IVTQTMKG (SEQ ID NO: 5) and VTQTMKGL (SEQ ID NO: 6). A peptide that comprises any one of amino acid sequences of SEQ ID NOS: 1, 4, 5 and 6. A method for producing a whey protein hydrolyzate containing a peptide that comprises any one of amino acid sequences of SEQ ID NOS: 1 to 6, said method comprising a step for hydrolyzing whey protein with chymotrypsin.

Description

[Technical Field]

[0001] The present invention relates to a novel peptide and a novel use of the peptide. The present invention also relates to a method for producing the peptide. [Background technology]

[0002] Patent Document 1 and Non-Patent Document 1 describe that a peptide having the amino acid sequence LIVTQTMKG (SEQ ID NO: 7) at its N-terminus has an inhibitory effect on ghrelin secretion and also has an appetite suppressant effect due to the suppression of ghrelin secretion. However, these documents do not describe the effects of improving intestinal barrier function, suppressing blood glucose elevation, improving insulin sensitivity, promoting FGF21 (fibroblast growth factor 21) secretion, suppressing stress, protecting the nerves, or reducing fatigue. Non-Patent Documents 2 and 3 show that ghrelin improves intestinal barrier function. Non-Patent Document 4 also describes, through a meta-analysis of past studies, that ghrelin improves insulin sensitivity. Therefore, the ghrelin secretion-inhibiting effect described in Patent Document 1 and Non-Patent Document 1 does not suggest that ghrelin also has effects of improving intestinal barrier function, improving insulin sensitivity, or suppressing blood glucose elevation. Furthermore, there are no published documents that show the relationship between ghrelin and FGF21. [Prior art documents] [Patent documents]

[0003] [Patent Document 1] International Publication No. 2017 / 150536 [Non-patent literature]

[0004] [Non-Patent Document 1] H. Aoki et al., “Lacto-ghrestatin, a novel bovine milk-derived peptide, suppresses ghrelin secretion”, FEBS Letters 591 (2017) 2121-2130 [Non-patent document 2] R. Wu et al., “Orexigenic Hormone Ghrelin Ameliorates Gut Barrier Dysfunction In Sepsis In Rats”, Critical Care Medicine, 2009 August; 37(8): 2421-2426; doi: 10.1097 / CCM.0b013e3181a557a2 [Non-patent document 3] Y. Cheng et al., “Ghrelin Attenuates Intestinal Barrier Dys Followingfunction Intracerebral Hemorrhage in Mice”, International Journal of Molecular Sciences, 2016, 17, 2032; doi: 10.3390 / ijms17122032 [Non-patent document 4] C. Zhang et al., “The Correlation Between Circulating Ghrelin and Insulin Resistance in Obesity: A Meta-Analysis”, Frontiers in Physiology, September 2018, Volume 9, Article 1308; doi: 10.3389 / fphys.2018.01308 Summary of the Invention [Problem to be solved by the invention]

[0005] An object of the present invention is to provide a novel peptide and a novel use of the peptide. Another object of the present invention is to provide a method for producing the peptide. [Means for solving the problem]

[0006] The present inventors analyzed the gastrointestinal contents of an animal model undergoing gastrointestinal bypass surgery after administration of whey (milk protein) and found for the first time that a peptide consisting of a specific amino acid sequence has the effects of improving intestinal barrier function, suppressing blood glucose elevation, improving insulin sensitivity, and promoting FGF21 secretion. Furthermore, the present inventors also found for the first time that a peptide consisting of this specific amino acid sequence has the effects of suppressing stress, protecting the nerves, and reducing fatigue. Based on these new findings, the present inventors conducted further intensive research and completed the present invention.

[0007] That is, the present invention is as follows. [1] An agent for improving intestinal barrier function, comprising a peptide consisting of any one of the amino acid sequences LIVTQTMKGL (SEQ ID NO: 1), LIVTQTMKG (SEQ ID NO: 2), LIVTQTMK (SEQ ID NO: 3), IVTQTMKGL (SEQ ID NO: 4), IVTQTMKG (SEQ ID NO: 5), and VTQTMKGL (SEQ ID NO: 6) (hereinafter, these may be collectively referred to as "peptides related to the present invention"). [2] An agent for suppressing blood glucose elevation, comprising a peptide consisting of any one of the amino acid sequences LIVTQTMKGL (sequence number 1), LIVTQTMKG (sequence number 2), LIVTQTMK (sequence number 3), IVTQTMKGL (sequence number 4), IVTQTMKG (sequence number 5), and VTQTMKGL (sequence number 6). [3] An agent for improving insulin sensitivity, comprising a peptide consisting of any one of the amino acid sequences LIVTQTMKGL (sequence number 1), LIVTQTMKG (sequence number 2), LIVTQTMK (sequence number 3), IVTQTMKGL (sequence number 4), IVTQTMKG (sequence number 5), and VTQTMKGL (sequence number 6). [4] An agent for promoting FGF21 secretion, comprising a peptide consisting of any one of the amino acid sequences LIVTQTMKGL (sequence number 1), LIVTQTMKG (sequence number 2), LIVTQTMK (sequence number 3), IVTQTMKGL (sequence number 4), IVTQTMKG (sequence number 5), and VTQTMKGL (sequence number 6). [5] A stress-suppressing or neuroprotective agent containing a peptide consisting of any one of the amino acid sequences LIVTQTMKGL (SEQ ID NO: 1), LIVTQTMKG (SEQ ID NO: 2), LIVTQTMK (SEQ ID NO: 3), IVTQTMKGL (SEQ ID NO: 4), IVTQTMKG (SEQ ID NO: 5), and VTQTMKGL (SEQ ID NO: 6). [6] A fatigue-reducing agent containing a peptide consisting of any one of the amino acid sequences LIVTQTMKGL (SEQ ID NO: 1), LIVTQTMKG (SEQ ID NO: 2), LIVTQTMK (SEQ ID NO: 3), IVTQTMKGL (SEQ ID NO: 4), IVTQTMKG (SEQ ID NO: 5), and VTQTMKGL (SEQ ID NO: 6). [7] A peptide consisting of any one of the amino acid sequences of LIVTQTMKGL (SEQ ID NO: 1), IVTQTMKGL (SEQ ID NO: 4), IVTQTMKG (SEQ ID NO: 5), and VTQTMKGL (SEQ ID NO: 6). [8] A method for producing a whey protein hydrolysate containing a peptide having an amino acid sequence selected from the group consisting of LIVTQTMKGL (SEQ ID NO: 1), LIVTQTMKG (SEQ ID NO: 2), LIVTQTMK (SEQ ID NO: 3), IVTQTMKGL (SEQ ID NO: 4), IVTQTMKG (SEQ ID NO: 5), and VTQTMKGL (SEQ ID NO: 6), comprising a step of hydrolyzing whey protein with chymotrypsin. [Effects of the Invention]

[0008] The peptides of the present invention have the effects of improving intestinal barrier function, suppressing blood glucose elevation, improving insulin sensitivity, promoting FGF21 secretion, suppressing stress, protecting the nerves, and reducing fatigue, and can be used as medicines, foods, etc. for applications based on these effects. [Brief explanation of the drawings]

[0009] [Figure 1] FIG. 1 shows the blood FD-4 concentration (Mean±SEM (n=5)) after oral administration of Compound 1 (a peptide consisting of the amino acid sequence of LIVTQTMKGL (SEQ ID NO: 1)) in Test Example 1. [Figure 2] FIG. 2 shows ΔAUC (Mean±SEM (n=6 to 7)) during the oral glycosylation test (OGTT) in Test Example 2. [Figure 3] FIG. 3 shows the time course of blood glucose levels from 0 minutes during an insulin tolerance test (ITT) in Test Example 3 (FIG. 3(a)) and ΔAUC (FIG. 3(b)) (Mean±SEM (n=4 to 6)). [Figure 4] FIG. 4 shows the blood FGF21 concentration (Mean±SEM (n=5)) in Test Example 4. [Figure 5] FIG. 5 shows the blood acetyl-L-carnitine concentration (Mean±SEM (n=3)) in Test Example 5. [Figure 6] FIG. 6 shows the blood ornithine / citrulline ratio (Mean±SEM (n=5)) in Test Example 6. [Figure 7] FIG. 7 shows the concentration of Compound 1 (Mean±SEM (n=2)) in Production Example 7. DETAILED DESCRIPTION OF THE INVENTION

[0010] The peptides of the present invention are the following (1) to (6). (1) A peptide consisting of the amino acid sequence of LIVTQTMKGL (SEQ ID NO: 1) (2) A peptide consisting of the amino acid sequence LIVTQTMKG (SEQ ID NO: 2) (3) A peptide consisting of the amino acid sequence of LIVTQTMK (SEQ ID NO: 3) (4) Peptide consisting of the amino acid sequence of IVTQTMKGL (SEQ ID NO: 4) (5) Peptide consisting of the amino acid sequence IVTQTMKG (SEQ ID NO: 5) (6) Peptide consisting of the amino acid sequence of VTQTMKGL (SEQ ID NO: 6) As described above, the peptides of the present invention have the effects of improving intestinal barrier function, suppressing blood glucose elevation, improving insulin sensitivity, promoting FGF21 secretion, suppressing stress, protecting the nerves, and reducing fatigue. In the agents of the present invention described below, only one type of peptide of the present invention may be used, or two or more types may be used in combination.

[0011] The peptides of the present invention can be used not only in their free form but also in the form of salts, hydrates, or solvates. The term "peptide" as used herein encompasses salts, hydrates, and solvates. Salts of the peptides of the present invention include salts that are acceptable for use as pharmaceuticals or foods, such as acid addition salts (e.g., inorganic acid salts such as hydrochloride, sulfate, nitrate, and phosphate; organic acid salts such as acetate, maleate, fumarate, citrate, malate, lactate, α-ketoglutarate, gluconate, and caprylate), metal salts (e.g., alkali metal salts such as sodium salt and potassium salt; alkaline earth metal salts such as magnesium salt and calcium salt; aluminum salt; zinc salt), and ammonium salts (e.g., salts with ammonium, tetramethylammonium, etc.).

[0012] In the present invention, the amino acids constituting the peptide may be either L- or D-amino acids.

[0013] The peptides of the present invention can be produced, for example, by the solid phase synthesis method shown below. The support used in solid-phase synthesis is usually a resin that can be bonded to the C-terminal carboxyl group of a peptide chain via a linker. Representative examples of such solid-phase supports include Wang resin, AM resin, and TGR resin. Amino acids used in solid-phase synthesis are preferably those in which the amino group in the main chain is protected with a 9-fluorenylmethylcarbonyl (Fmoc) group or a t-butoxycarbonyl (Boc) group, but are not limited to these. Furthermore, when a hydroxyl group, thiol group, amino group, carboxyl group, or the like is present in the side chain of an amino acid, it is preferable that these functional groups be protected with a protecting group other than an Fmoc group or a Boc group. Introduction of a protected amino acid into a support can be carried out by a known method. For example, a method using a carbodiimide condensing agent as a condensing agent can be mentioned. Examples of the carbodiimide condensing agent include dicyclohexylcarbodiimide (DCC), diisopropylcarbodiimide (DIPC), and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (WSCI). Examples of solvents that can be used in the reaction include DCM, tetrahydrofuran, and toluene. The reaction is preferably carried out at room temperature. The Fmoc group can be removed by adding a secondary amine to the protected amino acid-support obtained above. Dimethylformamide (DMF) is preferably used as the reaction solvent. While piperidine is typically used as the secondary amine, pyrrolidine, diethylamine, dibutylamine, diisopropylamine, etc. can also be used. The reaction can be carried out at a reaction temperature ranging from 0°C to the boiling point of the solvent, and is preferably carried out at room temperature. After the reaction, the support can be removed from the solvent by filtration or the like. The support with the amino acid introduced and the Fmoc residue removed is again swollen in DMF and reacted with the protected amino acid. Condensation agents include dicyclohexylcarbodiimide (DCC), diisopropylcarbodiimide (DIPC), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (WSCI), 1-[bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxide hexafluorophosphate (HATU), 1-hydroxybenzotriazole (HOBt), and 1-hydroxy-7-azabenzotriazole (HOAt), either alone or in combination. The reaction can be carried out at temperatures ranging from 0°C to the boiling point of the solvent, preferably at room temperature. Peptide chain elongation can be confirmed by the Kaiser test, and the support can be removed from the solvent by filtration or other methods after the reaction. The peptide can be cleaved from the support by a known method. For example, the peptide can be cleaved using a strong acid such as trifluoroacetic acid. At this time, the protecting groups of the side chains of each amino acid in the peptide may be removed at the same time.

[0014] The peptides of the present invention can also be produced by treating whey protein with a hydrolase. The proteolytic enzyme used to hydrolyze whey protein is not particularly limited, but is preferably an enzyme that has protease or peptidase activity and can be used in food production. Examples of such enzymes include chymotrypsin. In this production method, the whey protein that is the substrate for the enzyme reaction may be, for example, purified milk β-lactoglobulin, but is not limited to this, and milk or whey containing whey protein may also be used as the substrate as is. In the production method, the amount of proteolytic enzyme used is, for example, an amount such that the mass ratio of proteolytic enzyme to substrate (whey protein) (proteolytic enzyme:substrate) is 1:20 to 1:1000. The enzyme reaction time is, for example, 30 minutes to 24 hours, and preferably about 2 to 8 hours. The enzyme reaction temperature is, for example, 25 to 70° C., and preferably 37° C. The enzyme reaction is carried out, for example, at pH 5 to 9, and preferably pH 6 to 8. After the enzymatic reaction is completed, the enzyme is appropriately inactivated to obtain a whey protein hydrolysate containing the peptide of the present invention. The obtained hydrolysate can be used as is in the agent of the present invention described below, or the peptide of the present invention can be obtained by separating and purifying it by a known method.

[0015] In one aspect, the present invention relates to an agent for improving intestinal barrier function, which contains a peptide according to the present invention. In the present invention, the term "intestinal barrier function" refers to the function of preventing microorganisms from invading the intestinal tissues by physical barriers such as intestinal epithelial cells, mucus layer, and glycocalyx, or by secretion of molecules with antibacterial activity. In the present invention, the intestinal barrier function improving effect can be evaluated, for example, by the method of Test Example 1 described below or a method similar thereto. Based on its intestinal barrier function-improving effect, the agent for improving intestinal barrier function of the present invention is expected to be used for the prevention or treatment (improvement) of metabolic diseases, intestinal infections, cognitive decline, depression, stress, inflammatory diseases, various symptoms associated with aging, and cardiovascular diseases, as well as for extending lifespan and maintaining health.

[0016] In one aspect, the present invention relates to an agent for suppressing blood glucose elevation, which contains the peptide of the present invention. In the present invention, "blood glucose increase" refers to an increase in blood glucose level caused by eating food, and generally refers to an increase in blood glucose level that occurs within about 3 to 5 hours after eating. In the present invention, the blood glucose elevation suppressing effect can be evaluated, for example, by the method of Test Example 2 described below or a method similar thereto. Based on its blood glucose elevation suppressing effect, the blood glucose elevation suppressing agent of the present invention is expected to be used for the prevention or treatment (improvement) of metabolic diseases, cognitive decline, depression, stress, inflammatory diseases, various symptoms associated with aging, and cardiovascular diseases, as well as for extending lifespan and maintaining health.

[0017] In one aspect, the present invention relates to an agent for improving insulin sensitivity, which comprises a peptide according to the present invention. In the present invention, "insulin sensitivity" refers to the ease with which insulin acts in the body; when insulin sensitivity is high, insulin can exert its effects sufficiently, and when insulin sensitivity is low, insulin cannot exert its effects sufficiently. The actions of insulin include the actions of regulating glucose, lipid, and protein metabolism, and of inducing cell proliferation and differentiation. In the present invention, the insulin sensitivity improving effect can be evaluated, for example, by the method of Test Example 3 described below or a method similar thereto. Based on its insulin sensitivity improving effect, the insulin sensitivity improving agent of the present invention is expected to be used for the prevention or treatment (improvement) of metabolic diseases, cognitive decline, depression, stress, inflammatory diseases, various symptoms associated with aging, and cardiovascular diseases, as well as for extending lifespan and maintaining health.

[0018] In one aspect, the present invention relates to an agent for promoting FGF21 secretion, which comprises a peptide according to the present invention. FGF21 is an intercellular signaling factor produced mainly in the liver and other organs, and is involved in the regulation of proliferation, differentiation, and metabolism of various cells. In the present invention, the FGF21 secretion-promoting activity can be evaluated, for example, by the method of Test Example 4 described below or a method similar thereto. Based on its FGF21 secretion-promoting effect, the FGF21 secretion-promoting agent of the present invention is expected to be used for the prevention or treatment (improvement) of metabolic diseases, cognitive decline, depression, stress, inflammatory diseases, various symptoms associated with aging, and cardiovascular diseases, as well as for extending lifespan and maintaining health. Many studies have reported on the relationship between FGF21 and metabolic diseases, cognitive decline, depression, stress, inflammatory diseases, and cardiovascular diseases (e.g., EMBO Molecular Medicine (2018) 10, e8791; Hormones and Behavior 85 (2016) 86-95; Psychiatry Research 252 (2017) 111-113; Molecular Psychiatry (2015) 20, 215-223; Endocrinology, June 2012, 153(6), 2689-2700; Cellular Signaling 40 (2017) 10-21; Reviews in Endocrine and Metabolic Disorders, https: / / doi.org / 10.1007 / s11154-019-09488-x).

[0019] In one aspect, the present invention relates to a stress-suppressing or neuroprotective agent containing a peptide according to the present invention. In this invention, "stress reduction" refers to reducing the psychological, physical, and behavioral effects caused by "physical stressors" (heat, cold, noise, crowding, etc.), "chemical stressors" (pollutants, drugs, oxygen deficiency / excess, carbon monoxide, etc.), and "psychological / social stressors" (interpersonal relationships, work problems, family problems, etc.). In the present invention, "neuroprotection" refers to protecting the central and peripheral nerves from losing their function due to physical or chemical factors. In the present invention, the stress suppression effect and neuroprotective effect can be evaluated, for example, by the method of Test Example 5 described below or a method similar thereto. Based on its stress-suppressing or neuroprotective effect, the stress-suppressing or neuroprotective agent of the present invention is expected to be useful for preventing or treating (improving) cognitive decline, depression, stress, inflammatory diseases, symptoms associated with aging, and cardiovascular diseases, as well as for extending lifespan and maintaining health.

[0020] In one aspect, the present invention relates to an agent for reducing fatigue, which contains the peptide of the present invention. In the present invention, "fatigue reduction" refers to the reduction of fatigue, loss of motivation, loss of concentration, and the like caused by accumulated physical and mental loads. In the present invention, the fatigue-reducing effect can be evaluated, for example, by the method of Test Example 6 described below or a method similar thereto. Based on its fatigue-reducing effect, the fatigue-reducing agent of the present invention is expected to be used for the prevention or treatment (improvement) of cognitive decline, depression, stress, inflammatory diseases, symptoms associated with aging, and cardiovascular diseases, as well as for extending lifespan and maintaining health.

[0021] The intestinal barrier function improving agent, blood glucose elevation suppressing agent, insulin sensitivity improving agent, FGF21 secretion promoting agent, stress suppressing or neuroprotecting agent, and fatigue reducing agent of the present invention (hereinafter these may be collectively referred to as "agents of the present invention") may be the peptide of the present invention itself, or may be a composition (e.g., pharmaceutical composition, food composition) containing the peptide of the present invention and other ingredients (e.g., carriers acceptable as pharmaceuticals or foods). The term "agent" in the present invention is a concept that encompasses medicines and foods.

[0022] The agent of the present invention can be safely administered orally or parenterally to subjects such as humans, mammals (e.g., mice, rats, hamsters, rabbits, cats, dogs, cows, sheep, and monkeys), and birds (e.g., chickens and turkeys).

[0023] The agent of the present invention may be in any form, including, for example, powders, granules, tablets, capsules, liquids (e.g., solutions, suspensions, emulsions), beverages, jellies, puddings, yogurt, candies, chewing gums, and the like. These can be produced by known methods. For example, the peptide of the present invention can be mixed with a food- or pharmaceutically acceptable carrier (e.g., excipient, binder, disintegrant, lubricant, solvent) to produce powders, granules, tablets, capsules, liquids, and the like by known methods in the fields of food formulations or pharmaceutical formulations. Alternatively, the peptide of the present invention can be added to and mixed with food (e.g., general food, beverages (e.g., water, soft drinks)) to produce the agent. In this specification, food is a concept that broadly encompasses anything that can be taken orally (excluding pharmaceuticals), and includes not only so-called "food" but also beverages, health supplements, health functional foods (e.g., foods for specified health uses, foods with functional claims, foods with nutrient functions), supplements, etc.

[0024] In the agent for improving intestinal barrier function of the present invention, the dosage (intake) of the peptide of the present invention is, for example, 0.05 mg to 500 g, preferably 0.5 mg to 50 g, and more preferably 5 mg to 10 g per day for an adult (body weight 60 kg). In the blood glucose elevation suppressant of the present invention, the dose (intake) of the peptide of the present invention is, for example, 0.05 mg to 500 g, preferably 0.5 mg to 50 g, more preferably 5 mg to 10 g per day for an adult (body weight 60 kg). In the insulin sensitivity improving agent of the present invention, the dose (intake) of the peptide according to the present invention is, for example, 0.05 mg to 500 g, preferably 0.5 mg to 50 g, more preferably 5 mg to 10 g per day for an adult (body weight 60 kg). In the FGF21 secretion promoting agent of the present invention, the dosage (intake) of the peptide according to the present invention is, for example, 0.05 mg to 500 g, preferably 0.5 mg to 50 g, more preferably 5 mg to 10 g per day for an adult (body weight 60 kg). In the stress suppressing or neuroprotective agent of the present invention, the dosage (intake) of the peptide of the present invention is, for example, 0.05 mg to 500 g, preferably 0.5 mg to 50 g, more preferably 5 mg to 10 g per day for an adult (body weight 60 kg). In the fatigue-reducing agent of the present invention, the dosage (intake) of the peptide of the present invention is, for example, 0.05 mg to 500 g, preferably 0.5 mg to 50 g, and more preferably 5 mg to 10 g per day for an adult (body weight 60 kg). In the agent of the present invention, the content of the peptide of the present invention can be appropriately selected from amounts that ensure the dosage (intake) falls within the above range.

[0025] The present invention also relates to novel peptides consisting of any one of the amino acid sequences LIVTQTMKGL (SEQ ID NO: 1), IVTQTMKGL (SEQ ID NO: 4), IVTQTMKG (SEQ ID NO: 5), and VTQTMKGL (SEQ ID NO: 6). The definition and production method of each peptide are the same as those described above for the agent of the present invention. As described above, these peptides have the effects of improving intestinal barrier function, suppressing blood glucose elevation, improving insulin sensitivity, promoting FGF21 secretion, suppressing stress, providing neuroprotection, or reducing fatigue, and can be used as medicines, foods, and the like for applications based on these effects. [Example]

[0026] The present invention will be explained in more detail below by way of Production Examples and Test Examples, but the present invention is not limited to these Production Examples and Test Examples. In the following Production Examples and Test Examples, Compounds 1 to 6 are as follows. Compound 1: A peptide consisting of the amino acid sequence LIVTQTMKGL (SEQ ID NO: 1) Compound 2: A peptide consisting of the amino acid sequence LIVTQTMKG (SEQ ID NO: 2) Compound 3: A peptide consisting of the amino acid sequence of LIVTQTMK (SEQ ID NO: 3) Compound 4: A peptide consisting of the amino acid sequence IVTQTMKGL (SEQ ID NO: 4) Compound 5: A peptide consisting of the amino acid sequence IVTQTMKG (SEQ ID NO: 5) Compound 6: A peptide consisting of the amino acid sequence VTQTMKGL (SEQ ID NO: 6)

[0027] [Production Example 1] Production of Compound 1 The target protected peptide resin was synthesized using Fmoc-Leu-Wang resin as a starting material by elongating the peptide chain using the 9-fluorenylmethoxycarbonyl (Fmoc) method. The resin was then deprotected and deprotected using trifluoroacetic acid to obtain the crude peptide. The resulting crude peptide was purified by high-performance liquid chromatography (HPLC) and lyophilized to obtain the target product as a white powder. Yield: 3.0g (purity: 98.3%) ESI-MS: MW=1103.0 (theoretical value 1103.4) Amino acid analysis: Thr (2) 1.90, Glu (1) 1.01, Gly (1) 0.98, Val (1) 0.80, Met (1) 0.97, Ile (1) 0.79, Leu (2) 2.0, Lys (1) 1.01, NH3 (1) 1.10

[0028] [Production Example 2] Production of Compound 2 The target protected peptide resin was synthesized using Fmoc-Gly-Wang resin as a starting material by elongating the peptide chain using the 9-fluorenylmethoxycarbonyl (Fmoc) method. The resin was then deprotected and deprotected using trifluoroacetic acid to obtain the crude peptide. The resulting crude peptide was purified by high-performance liquid chromatography (HPLC) and lyophilized to obtain the target product as a white powder. Yield: 1.26g (purity: 99.3%) ESI-MS: MW=990.2 (theoretical value 990.2) Amino acid analysis: Thr (2) 1.89, Glu (1) 1.00, Gly (1) 0.97, Val (1) 0.81, Met (1) 0.98, Ile (1) 0.80, Leu (1) 0.99, Lys (1) 1.01, NH3 (1) 1.10

[0029] [Production Example 3] Production of Compound 3 The target protected peptide resin was synthesized using Fmoc-Lys(Boc)-Wang resin as a starting material by the 9-fluorenylmethoxycarbonyl (Fmoc) method. The resin was then deprotected by trifluoroacetic acid treatment to obtain the crude peptide. The resulting crude peptide was purified by high-performance liquid chromatography (HPLC) and lyophilized to obtain the target product as a white powder. Yield: 1.26g (purity: 99.0%) ESI-MS: MW=933.1 (theoretical value 933.2) Amino acid analysis: Thr (2) 1.89, Glu (1) 1.00, Val (1) 0.81, Met (1) 0.98, Ile (1) 0.80, Leu (1) 0.99, Lys (1) 1.01, NH3 (1) 1.10

[0030] [Production Example 4] Production of Compound 4 The target protected peptide resin was synthesized using Fmoc-Leu-Wang resin as a starting material by elongating the peptide chain using the 9-fluorenylmethoxycarbonyl (Fmoc) method. The resin was then deprotected and deprotected using trifluoroacetic acid to obtain the crude peptide. The resulting crude peptide was purified by high-performance liquid chromatography (HPLC) and lyophilized to obtain the target product as a white powder. Yield: 1.26g (purity: 99.4%) ESI-MS: MW=990.0 (theoretical value 990.2) Amino acid analysis: Thr (2) 1.89, Glu (1) 1.00, Gly (1) 0.99, Val (1) 0.68, Met (1) 0.98, Ile (1) 0.67, Leu (1) 1.00, Lys (1) 1.02, NH3 (1) 1.12

[0031] [Production Example 5] Production of Compound 5 The target protected peptide resin was synthesized using Fmoc-Gly-Wang resin as a starting material by elongating the peptide chain using the 9-fluorenylmethoxycarbonyl (Fmoc) method. The resin was then deprotected and deprotected using trifluoroacetic acid to obtain the crude peptide. The resulting crude peptide was purified by high-performance liquid chromatography (HPLC) and lyophilized to obtain the target product as a white powder. Yield: 1.26g (purity: 99.3%) ESI-MS: MW=876.9 (theoretical value 877.1) Amino acid analysis: Thr (2) 1.88, Glu (1) 1.00, Gly (1) 0.97, Val (1) 0.66, Met (1) 0.99, Ile (1) 0.65, Leu (1) 1.00, Lys (1) 1.01, NH3 (1) 1.09

[0032] [Production Example 6] Production of Compound 6 The target protected peptide resin was synthesized using Fmoc-Leu-Wang resin as a starting material by elongating the peptide chain using the 9-fluorenylmethoxycarbonyl (Fmoc) method. The resin was then deprotected and deprotected using trifluoroacetic acid to obtain the crude peptide. The resulting crude peptide was purified by high-performance liquid chromatography (HPLC) and lyophilized to obtain the target product as a white powder. Yield: 1.26g (purity: 99.5%) ESI-MS: MW=877.0 (theoretical value 877.1) Amino acid analysis: Thr (2) 1.87, Glu (1) 1.00, Gly (1) 0.98, Val (1) 1.00, Met (1) 0.99, Ile (1) 1.00, Leu (1) 1.00, Lys (1) 1.01, NH3 (1) 1.33

[0033] [Test Example 1] Intestinal permeability Male KK-Ay mice (11 weeks old) were fasted from 10:00 the previous day and orally administered 100 mg / kg of the administration vehicle (0.5% methylcellulose; vehicle) or Compound 1, followed by orally administered 300 mg / kg of FITC-dextran (FD-4) one hour later. Blood was collected from the tail vein one and two hours after FD-4 administration, and the plasma FD-4 concentration was measured. The results are shown in Figure 1. As shown in Figure 1, the increase in blood FD-4 concentration was suppressed in the compound 1 group compared to the vehicle group both 1 and 2 hours after oral administration of FD-4, confirming that compound 1 improves intestinal barrier function.

[0034] [Test Example 2] Oral glucose tolerance test (OGTT) Male C57BL / 6J mice (7 weeks old) were fasted from 5 PM the previous day and orally administered 30 mg / kg of the administration vehicle (0.5% methylcellulose; vehicle) or Compound 1. One hour later, 2 g / kg of glucose was orally administered by force. Blood was collected from the tail vein before administration and 15, 30, 60, 120, and 180 minutes after administration, and blood glucose levels were measured. After glucose loading, blood glucose levels remained low from 30 minutes after loading in the group administered Compound 1. The area under the blood glucose level transition curve (ΔAUC) was calculated using the value at 0 minutes as the reference, and the results are shown in Figure 2. As can be seen from FIG. 2, the compound 1 administration group showed lower values compared to the vehicle group, confirming the blood glucose elevation inhibitory effect of compound 1.

[0035] [Test Example 3] Insulin Tolerance Test (ITT) Male KK-Ay mice (7-12 weeks old) were fasted from 5 PM the previous day and orally administered 30 mg / kg of the administration vehicle (0.5% methylcellulose; vehicle) or compound 1, followed by subcutaneous administration of insulin (0.5 U / kg) 1 hour later. Blood was collected from the tail vein before administration and 15, 30, 60, 120, and 180 minutes after administration, and blood glucose levels were measured. Blood glucose levels were also measured for compounds 2-6 in the same manner. The time course of blood glucose levels from 0 minutes is shown in Figure 3(a). The area under the blood glucose curve (ΔAUC) calculated based on the value at 0 minutes is shown in Figure 3(b). 3(a) and (b), KK-Ay mice have strong insulin resistance, and although no clear decrease in blood glucose level was observed in the vehicle group even after insulin administration, a significant decrease in blood glucose level was observed in the group administered with Compound 1. Such an insulin sensitivity improving effect was also observed in Compounds 2 to 6.

[0036] [Test Example 4] FGF21 secretion promoting effect Male C57BL / 6J mice (8 weeks old) were fasted for 6 hours and then given 30 mg / kg of Compound 1 or 0.5% methylcellulose (vehicle) by oral gavage. Blood samples were taken from the portal vein before and 120 minutes after administration, and plasma FGF21 concentrations were measured. The results are shown in Figure 4. As shown in FIG. 4, an increase in blood FGF21 concentration 120 minutes after administration was confirmed in the Compound 1 administration group compared to the vehicle group.

[0037] [Test Example 5] Anti-stress and neuroprotective effects Male C57BL / 6J mice (9 weeks old) were fasted overnight and placed in a mouse restraint stress cage. After 3 hours, the mice were orally administered with 0.5% methylcellulose (vehicle) or 30 mg / kg of Compound 1. Blood was collected from the inferior vena cava 120 minutes after administration, and plasma acetyl-L-carnitine concentrations were measured. Acetyl-L-carnitine is an endogenous metabolite known to have anti-stress and neuroprotective properties (e.g., Nasca C et al., “L-acetylcarnitine causes rapid antidepressant effects through the epigenetic induction of mGlu2 receptors”, Proc. Natl. Acad. Sci. USA, 2013, Mar 19; 110(12): 4804-4809; and Kazak F et al., “Neuroprotective effects of acetyl-L-carnitine on lipopolysaccharide-induced neuroinflammation in mice: Involvement of brain-derived neurotrophic factor”, Neuroscience Letters, 2017 Sep 29; 658: 32-36). The results are shown in Figure 5. As shown in FIG. 5, an increase in blood acetyl-L-carnitine concentration was observed 120 minutes after administration in the Compound 1 administration group compared to the vehicle group, confirming a stress-suppressing or neuroprotective reaction.

[0038] [Test Example 6] Anti-fatigue effect Male C57BL / 6J mice (7 weeks old) were fasted overnight and administered 30 mg / kg of compound 1 or 0.5% methylcellulose (vehicle) or compound 1 by oral gavage. Blood was collected from the portal vein 120 minutes after administration, and plasma ornithine and citrulline concentrations were measured. The plasma ornithine / citrulline ratio is known to be elevated in fatigue conditions such as chronic fatigue syndrome (e.g., Yamano E et al., “Index markers of chronic fatigue syndrome with dysfunction of TCA and urea cycles”, Scientific Reports, 2016 Oct 11; 6: 34990, doi: 10.1038 / srep34990). The results are shown in Figure 6. As can be seen from FIG. 6, the blood ornithine / citrulline ratio was lower in the Compound 1 administration group 120 minutes after administration compared to the vehicle group, and a biological reaction that reduced fatigue was observed.

[0039] [Production Example 7] Production of milk protein hydrolysate Purified bovine milk β-lactoglobulin (final concentration 2 mg / mL) and chymotrypsin (final concentration 0.02 mg / mL) were mixed in 10 mM phosphate buffer (pH 6.0) and reacted at 37°C. After the start of the reaction, the reaction solution was sampled over time, and the concentration of Compound 1 in the reaction solution was measured by Q-TOF / MS. The results are shown in Figure 7. 7, it was confirmed that compound 1 was produced in the reaction solution in a reaction time-dependent manner. Therefore, it was confirmed that the compound can also be produced by enzymatic hydrolysis of milk proteins. [Industrial Applicability]

[0040] The peptides of the present invention are useful as medicines and foods for improving intestinal barrier function, suppressing blood glucose elevation, improving insulin sensitivity, promoting FGF21 secretion, suppressing stress, providing neuroprotection, and alleviating fatigue.

[0041] This application is based on patent application No. 2019-109089 filed in Japan, the contents of which are incorporated in full herein.

Claims

1. An agent for improving intestinal barrier function, comprising a peptide consisting of the amino acid sequence LIVTQTMKGL (sequence number 1).

2. An agent for suppressing blood glucose elevation, containing as an active ingredient only a peptide consisting of the amino acid sequence LIVTQTMKGL (SEQ ID NO: 1).

3. An agent for improving insulin sensitivity, containing as an active ingredient only a peptide consisting of any one of the amino acid sequences LIVTQTMKGL (SEQ ID NO: 1), LIVTQTMKG (SEQ ID NO: 2), LIVTQTMK (SEQ ID NO: 3), IVTQTMKGL (SEQ ID NO: 4), IVTQTMKG (SEQ ID NO: 5), or VTQTMKGL (SEQ ID NO: 6).

4. An agent for promoting FGF21 secretion, comprising a peptide consisting of the amino acid sequence LIVTQTMKGL (sequence number 1).

5. An agent for suppressing stress or providing neuroprotection by increasing blood acetyl-L-carnitine concentration, which comprises a peptide consisting of the amino acid sequence LIVTQTMKGL (SEQ ID NO: 1).

6. A fatigue-reducing agent containing, as an active ingredient, only a peptide consisting of the amino acid sequence LIVTQTMKGL (SEQ ID NO: 1).

7. A fatigue-reducing agent as described in claim 6, wherein the fatigue reduction is a reduction in loss of motivation.

8. A fatigue-reducing agent as described in claim 6, wherein the fatigue reduction is a reduction in decreased concentration.

9. A peptide consisting of the amino acid sequence VTQTMKGL (SEQ ID NO: 6).

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