Coacervate core-shell microcapsules

Abstract

The present invention relates to plant-based coacervate core-shell microcapsules, wherein the shell comprises a plant protein extract, and methods and uses thereof.

Description

[Technical Field]

[0001] The present invention relates to plant-based coacervate core-shell microcapsules, wherein the shell comprises a plant protein extract, and methods and uses thereof.

[0002] Background technology Typical steps in the coacervation process include: (a) emulsification of a generally hydrophilic material in a solution containing hydrocolloids; (b) coacervation (phase separation), which refers to the formation of a coacervate phase; (c) wall formation by aggregation of hydrocolloids around droplets of the emulsified hydrophobic material; and (d) hardening of the wall, which is generally achieved by cross-linking the wall-forming hydrocolloids to make the process irreversible and the resulting microcapsules insoluble in water and resistant to mechanical stress and thermal exposure.

[0003] The wall formation step is generally driven by the difference in surface tension between the coacervate phase, water, and the hydrophobic material. In most coacervation processes, one of the hydrocolloids used in the coacervation process is gelatin. The reasons for this traditional choice are: (i) gelatin is generally easy to use, since its rheological behavior, structure, and molecular arrangement in solution and in gelled form can be easily controlled using parameters of concentration and temperature (see, for example, the publication by Norman et al., "Gelation Kinetics of Gelatin: A Master Curve and Network Modeling," Macromolecules, 2004). 2000, 33, 3, 1063-1071), (ii) control over these parameters allows the operator to select process and formulation conditions such that the resulting coacervate remains liquid and deposits on the payload material to be encapsulated; (iii) the resulting gelatin-containing coacervate shell can be readily gelled by lowering the process temperature, thereby rapidly providing an initial physically cross-linked shell that protects the newly formed capsules against shear and mixing; and (iv) the gelatin gelation process induced by lowering the temperature is fully reversible as the phase separation step in which the coacervate is formed allows the operator to select process and formulation conditions such that the resulting coacervate remains liquid and deposits on the payload material to be encapsulated. (v) gelatin has ideal electrostatic properties for interacting with non-gelatin polymers during coacervation, thereby avoiding the formation of undesirable and intractable precipitates or other types of aggregates; (vi) gelatin exhibits ideal amphiphilic properties due to the protein's primary and secondary structure imparted by its amino acid composition and sequence; and (vii) gelatin is less likely to aggregate after wall formation when the temperature is below its gelation temperature compared to non-gelling hydrocolloids. All these aspects are inherent advantages of gelatin, which are directly related to its molecular structure, since it is derived from hydrolyzed animal collagen.

[0004] However, the use of capsules containing gelatin is not possible in food consumer products or foods where animal-derived ingredients are not permitted due to regulations, potential health hazards (such as mad cow disease or bovine spongiform encephalopathy), cultural restrictions, or religious restrictions. Furthermore, it is often desirable to use plant-based ingredients compared to animal-derived ingredients for a better sustainability profile of the final consumer product, primarily because the water requirement for producing a comparable amount of protein derived directly from plants is much more limited compared to protein derived from animal breeding. Since the combination of all the above-mentioned advantages (i) to (vii) is generally considered impossible to obtain by simply replacing gelatin with protein or non-protein biopolymers or other food-grade ingredients, it is very difficult to simply replace gelatin with plant biopolymers to obtain coacervates with comparable properties. In particular, the majority of plant proteins suitable for use as ingredients in food and consumer products do not possess the required electrostatic, amphiphilic, and gelling properties. In particular, vegetable proteins, and even dairy proteins, cannot be prepared in heated solutions that turn into gels upon cooling, but rather form particulate gels that are partially or completely irreversible (a similar illustrative example for proteins of animal origin is the coagulation of albumin, which is widely known to occur irreversibly upon heating egg whites).

[0005] Therefore, it is desirable to provide new plant-based coacervate microcapsules and also to establish various methods for producing microcapsules by coacervation. [Brief explanation of the drawings]

[0006] [Figure 1] Coacervate core-shell microcapsules according to the present invention [Figure 2](a) SDS-PAGE of an extract obtained from soy flour and the resulting complex coacervate with gum arabic, and (b) SDS-PAGE of an extract obtained from pea flour and the resulting complex coacervate with gum arabic. [Figure 3] Rheology experiments and images of soy polymer / gum arabic complex coacervates cured by heating. Temperature gradient of soy polymer / gum arabic complex coacervates. Shown are the elastic modulus (G') and viscous modulus (G'') upon heating and subsequent cooling. Filled symbols: G', empty symbols: G''.

[0007] Detailed Description of the Invention Unless otherwise specified, percentages (%) are meant to indicate weight percentages of a composition.

[0008] A first object of the present invention is a coacervate core-shell microcapsule containing a hydrophobic material, preferably a flavor or fragrance, wherein: - the hydrophobic material is encapsulated in the core of the coacervate core-shell microcapsules; and The shell of the coacervate core-shell microcapsules comprises at least one plant protein extract and optionally a non-protein polymer.

[0009] Another object of the present invention is a coacervate core-shell microcapsule slurry comprising at least one coacervate core-shell microcapsule containing a hydrophobic material, preferably a flavor or fragrance, wherein: - the hydrophobic material is encapsulated in the core of the coacervate core-shell microcapsules; and The shell of the coacervate core-shell microcapsules comprises at least one plant protein extract and optionally a non-protein polymer.

[0010] By "coacervate core-shell microcapsules" is meant to be understood microcapsules that comprise an oily or solid core material ("hydrophobic material") surrounded by a hydrogel shell that comprises a coacervate material (also called a "membrane" or "coating layer"). The core material may be partially or completely covered by the hydrogel shell.

[0011] Preferably, the coacervate core-shell microcapsules of the present invention comprise a core completely surrounded by a coacervate shell. According to this embodiment, the core is understood to be completely encapsulated by the coacervate shell.

[0012] Preferably, the coacervate core-shell microcapsules have a degree of cross-linking of 10 to 70% according to the method described in Soft Matter, 2011, 7, 3315-3322 (Determination of covalent cross-linker efficacy of gelatin strands using calorimetric analyses of the gel state).

[0013] According to certain embodiments, the coacervate core-shell microcapsules have a bursting force of 0.01 to 10 N, preferably 0.1 to 2 N. The bursting force can be measured by compressing the capsules between the parallel plates of a mechanical testing instrument, such as a Texture Analyzer (Food Technology Corporation, USA), an Instron Mechanical Testing machine (Instron, USA), or using a rheometer device equipped with normal force (e.g., a DHR-2 Rheometer manufactured by TA Instruments, USA, or an MCR Rheometer manufactured by Anton Paar GmbH, Germany).

[0014] The coacervate core-shell microcapsules may have a median capsule size of 5 to 1000 μm, preferably 100 to 800 μm, more preferably 200 to 600 μm, and even more preferably 250 to 450 μm. The median microcapsule size of the coacervate core-shell microcapsules can be determined by standard laser diffraction particle size analysis or by optical microscopy combined with image analysis. For purposes of the present invention, microcapsule size refers to a number-based size distribution measured by optical microscopy (e.g., with a Nikon TE2000 microscope) and image analysis (implemented with Nikon NIS Elements software). Methods for obtaining median and average size distributions are described in the scientific literature (e.g., R.J. Hunter, "Introduction to Modern Colloid Science," Oxford University Press, 1994).

[0015] Coacervate core-shell microcapsules may be made by "simple" and "complex" coacervation. By simple coacervation, it is understood that only the plant protein extract undergoes phase separation and is then used to form the capsule wall. By complex coacervation, it is understood that a non-protein polymer and the plant protein extract are combined to form the microcapsule shell.

[0016] According to certain embodiments, coacervate core-shell microcapsules are produced by "complex" coacervation.

[0017] According to the present invention, the shell of the core-shell microcapsules comprises at least one plant protein extract. The shell may comprise a plant protein extract or a mixture of plant protein extracts.

[0018] With regard to "plant protein extract" it is to be understood that it is preferably an extract obtained from the extraction of pulse seeds, pulse flour or root vegetables, more preferably an extract obtained from the acidic extraction of pulse seeds, pulse flour or root vegetables.

[0019] According to one embodiment, the plant protein extract is an extract obtained from the extraction of pulse seeds, preferably from the acidic extraction of pulse seeds.

[0020] According to one embodiment, the plant protein extract is a legume extract and is obtained from the extraction of legume flour.

[0021] According to one embodiment, the plant protein extract is an acidic plant protein extract.

[0022] According to one embodiment, the plant protein extract is a basic plant protein extract.

[0023] According to the present invention, the terms "plant protein extract" or "plant protein concentrate" are used interchangeably. Typically, the protein content in the extract (or concentrate) is less than 55% by weight.

[0024] In contrast to isolates, extracts are not obtained by using further purification steps (e.g., ion exchange or membrane filtration) beyond the extraction step itself. According to the present invention, the plant protein extract is not a plant protein isolate. Indeed, in contrast to isolates, which contain a large amount of protein (typically more than 80%), the protein content contained in the extract (or concentrate) is less than 55% by weight. In contrast to isolates, extracts may retain additional components that are lost during the preparation of isolates, such as soluble and insoluble carbohydrates and lipids, including phospholipids. Such additional components may be advantageous for providing density and / or nutritional value to the prepared material. According to one embodiment, the plant protein extract comprises carbohydrates and / or lipids.

[0025] Indeed, a key advantage of the present invention compared to the use of known protein isolates is that the gentle extraction of the pulse flour (or pulse seeds or root vegetables) does not damage and denature the proteins, leaving them sufficiently intact to undergo complex coacervation.

[0026] According to one embodiment, the plant protein extract is selected from the group consisting of protein extracts from soybean, pea, wheat, rice, potato, quinoa, amaranth, lentil, hemp, oat, buckwheat, chickpea, lupin seed, canola, flaxseed and mixtures thereof.

[0027] According to one embodiment, the extract contains globulins in an amount of 0-75% (w / w), preferably 5-35% (w / w).

[0028] According to one embodiment, the shell of the core-shell microcapsules comprises a non-protein polymer in addition to the plant protein extract.

[0029] The non-protein polymer may be selected from the group consisting of gum arabic, carboxymethylcellulose, chitosan, xanthan, agar, alginate, pectinate or carrageenan, preferably the non-protein polymer is gum arabic.

[0030] According to a particular embodiment, the non-protein polymer is chitosan.

[0031] Further preferred non-proteins may be derived from the literature, for example De Kruif et al., Current Opinion in Colloid and Interface Science, Vol. 9, pp 340-349, 2004.

[0032] According to a particular embodiment, the plant protein extract is a soy protein extract and the non-protein polymer is gum arabic.

[0033] According to one embodiment, the mass ratio between plant protein extract and non-protein polymer is between 1 and 100, in particular between 1 and 10, more in particular between 1 and 5.

[0034] According to a particular embodiment, - the plant protein extract is soy protein extract and the non-protein polymer is gum arabic; and The mass ratio of the plant protein extract to the non-protein polymer is 1:0.7 to 1:0.3.

[0035] According to a particular embodiment, - the plant protein extract is a pea protein extract and the non-protein polymer is gum arabic; and The mass ratio of the plant protein extract to the non-protein polymer is 1:0.3 to 1:0.1.

[0036] According to one embodiment, the coacervate shell is free of animal protein.

[0037] According to one embodiment, the coacervate shell is gelatin-free.

[0038] In accordance with the present invention, the coacervate core-shell microcapsules comprise a hydrophobic material.

[0039] According to one embodiment, the hydrophobic material is a hydrophobic active ingredient.

[0040] By "hydrophobic active ingredient" is meant any hydrophobic active ingredient (single ingredient or mixture of ingredients) that forms a two-phase dispersion when mixed with water. The hydrophobic active ingredient is preferably liquid at about 20°C.

[0041] By "active ingredient" is meant a single compound or a combination of ingredients.

[0042] By "perfume or flavor oil" is meant a single perfuming or flavoring compound or a mixture of several perfuming or flavoring compounds.

[0043] The hydrophobic active ingredient is preferably selected from the group consisting of flavors, flavor ingredients, fragrances, fragrance ingredients, dietary supplements, cosmetics, pesticides, biocides, and mixtures thereof.

[0044] According to a particular embodiment, the hydrophobic active ingredient is a mixture of fragrances and other ingredients selected from the group consisting of functional foods, cosmetics, pesticides and biocide active agents.

[0045] According to a particular embodiment, the hydrophobic active ingredient is a mixture of a biocidal active agent and other ingredients selected from the group consisting of fragrances, functional foods, cosmetics, pesticides.

[0046] According to a particular embodiment, the hydrophobic active ingredient is a mixture of a pesticide and other ingredients selected from the group consisting of fragrances, functional foods, cosmetics, and biocide active agents.

[0047] According to certain embodiments, the hydrophobic active ingredient comprises a fragrance.

[0048] According to a particular embodiment, the hydrophobic active ingredient consists of a fragrance.

[0049] According to certain embodiments, the hydrophobic active ingredient comprises a flavor.

[0050] According to a particular embodiment, the hydrophobic active ingredient consists of a flavor.

[0051] By "perfume" (or "perfume oil"), we mean here an ingredient or composition that is preferably liquid at about 20°C. According to any one of the above embodiments, the perfume oil may be a perfuming ingredient alone or a mixture of perfuming ingredients in the form of a perfuming composition. By "perfuming ingredient" we mean here a compound that is used for the primary purpose of imparting or modifying an odor. In other words, such an ingredient to be considered a perfuming ingredient must be recognized by those skilled in the art as being at least capable of imparting or modifying the odor of the composition in a positive or desirable way, and not merely possessing a single odor. For the purposes of the present invention, perfume oil also includes combinations of perfuming ingredients, such as perfume precursors, emulsions or dispersions, with substances that together improve, enhance or modify the delivery of the perfuming ingredient, as well as combinations that provide additional benefits beyond modifying or imparting an odor, such as longevity, blooming, malodor neutralization, antimicrobial effect, microbial stability, pest control.

[0052] The nature and type of perfuming ingredients present in the oil phase, which will in any case not be exhaustive, do not warrant a more detailed description here; those skilled in the art can select said base based on their general knowledge and according to the intended use or application and the desired organoleptic effect. In general terms, these perfuming ingredients belong to various chemical families, such as alcohols, aldehydes, ketones, esters, ethers, acetates, nitriles, terpenoids, nitrogen or sulfur heterocyclic compounds, and essential oils, and said perfuming co-ingredients may be of natural or synthetic origin. Many of these co-ingredients are in any case listed in references, such as S. Arctander's *Perfume and Flavor Chemicals*, 1969, Montclair, New Jersey, USA, or its latest edition, or other works of a similar nature, as well as in the abundant patent literature in the field of perfumery. It is also understood that said ingredients may be compounds known to release various types of perfuming ingredients in a controlled manner.

[0053] Mention may in particular be made of perfuming ingredients customarily used in perfume formulations, such as: aldehyde components: decanal, dodecanal, 2-methyl-undecanal, 10-undecanal, octanal, nonanal and / or nonenal; - Aromatic herbal ingredients: eucalyptus oil, camphor, eucalyptol, 5-methyltricyclo[6.2.1.0~2,7~]undecane-4-one, 1-methoxy-3-hexanethiol, 2-ethyl-4,4-dimethyl-1,3-oxathiane, 2,2,7 / 8,9 / 10-tetramethylspiro[5.5]undec-8-en-1-one, menthol and / or alpha-pinene; - Balsam ingredients: coumarin, ethyl vanillin and / or vanillin; - Citrus components, dihydromyricenol, citral, orange oil, linalyl acetate, citronellyl nitrile, orange terpenes, limonene, 1-P-menthen-8-yl acetate and / or 1,4(8)-P-menthadiene; - Floral ingredients: Methyl dihydrojasmonate, linalool, citronellol, phenylethanol, 3-(4-tert-butylphenyl)-2-methylpropanal, hexyl cinnamaldehyde, benzyl acetate, benzyl salicylate, tetrahydro-2-isobutyl-4-methyl-4(2H)-pyranol, beta-ionone, methyl 2-(methylamino)benzoate, (E)-3-methyl-4-(2,6,6-trimethyl-2-cyclohexen-1-yl)-3-buten-2-one, (1E)-1-(2,6,6-trimethyl-2- (2E)-1-(2,6,6-trimethyl-2-cyclohexen-1-yl)-1-penten-3-one, 1-(2,6,6-trimethyl-1,3-cyclohexadien-1-yl)-2-buten-1-one, (2E)-1-(2,6,6-trimethyl-2-cyclohexen-1-yl)-2-buten-1-one, (2E)-1-[2,6,6-trimethyl-3-cyclohexen-1-yl]-2-buten-1-one, (2E)-1-(2,6,6-trimethyl-1-cyclohexen-1-yl)-2-buten-1-one, 2,5-dimethyl-2-indanethanol, 2,6,6-trimethyl-3-cyclohexen Cyclohexene-1-carboxylate, 3-(4,4-dimethyl-1-cyclohexen-1-yl)propanal, hexyl salicylate, 3,7-dimethyl-1,6-nonadien-3-ol, 3-(4-isopropylphenyl)-2-methylpropanal, vergyl acetate, geraniol, p-mentha-1-en-8-ol, 4-(1,1-dimethylethyl)-1-cyclohexyl acetate, 1,1-dimethyl-2-phenylethyl acetate, 4-cyclohexyl-2-methyl-2-butanol, amyl salicylate, high cis-methyldihydrochloride Rojasmonate, 3-methyl-5-phenyl-1-pentanol, vergyl propionate, geranyl acetate, tetrahydrolinalool, cis-7-P-methanol, propyl (S)-2-(1,1-dimethylpropoxy)propanoate, 2-methoxynaphthalene, 2,2,2-trichloro-1-phenylethyl acetate, 4 / 3-(4-hydroxy-4-methylpentyl)-3-cyclohexene-1-carbaldehyde, amyl cinnamaldehyde, 8-decen-5-olide, 4-phenyl-2-butanone, isononyl acetate, 4-(1,1-dimethylethyl)-1-cyclohexyl acetate, vergyl isobutyrate and / or a mixture of methyl ionone isomers; - Fruity ingredients: gamma-undecalactone, 2,2,5-trimethyl-5-pentylcyclopentanone, 2-methyl-4-propyl-1,3-oxathiane, 4-decanolide, ethyl 2-methylpentanoate, hexyl acetate, ethyl 2-methylbutanoate, gamma-nonalactone, allylheptanoate, 2-phenoxyethyl isobutyrate, ethyl 2-methyl-1,3-dioxolane-2-acetate, 3-(3,3 / 1,1-dimethyl-5-indanyl)propanal, diethyl 1,4-cyclohexanedicarboxylate, 3-methyl-2-hexen-1-yl acetate, 1-[3,3-dimethylcyclohexyl]ethyl[3-ethyl-2-oxiranyl]acetate and / or diethyl 1,4-cyclohexanedicarboxylate; - green ingredients: 2-methyl-3-hexanone (E)-oxime, 2,4-dimethyl-3-cyclohexene-1-carbaldehyde, 2-tert-butyl-1-cyclohexyl acetate, styrallyl acetate, allyl (2-methylbutoxy) acetate, 4-methyl-3-decen-5-ol, diphenyl ether, (Z)-3-hexen-1-ol and / or 1-(5,5-dimethyl-1-cyclohexen-1-yl)-4-penten-1-one; - Musk ingredients: 1,4-dioxa-5,17-cycloheptadecanedione, (Z)-4-cyclopentadecen-1-one, 3-methylcyclopentadecanone, 1-oxa-12-cyclohexadecen-2-one, 1-oxa-13-cyclohexadecen-2-one, (9Z)-9-cycloheptadecen-1-one, 2-{1S)-1-[(1R)-3,3-dimethylcyclohexyl]ethoxy}-2-oxaethylpropionate, 3-methyl-5-cyclopentadecanone entadecen-1-one, 1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexamethyl-cyclopenta-g-2-benzopyran, (1S,1'R)-2-[1-(3',3'-dimethyl-1'-cyclohexyl)ethoxy]-2-methylpropylpropanoate, oxacyclohexadecan-2-one and / or (1S,1'R)-[1-(3',3'-dimethyl-1'-cyclohexyl)ethoxycarbonyl]methylpropanoate; - Woody Ingredients: 1-[(1RS,6SR)-2,2,6-trimethylcyclohexyl]-3-hexanol, 3,3-dimethyl-5-[(1R)-2,2,3-trimethyl-3-cyclopenten-1-yl]-4-penten-2-ol, 3,4'-dimethylspiro[oxirane-2,9'-tricyclo[6.2.1.02,7]undec[4]ene, (1-ethoxyethoxy)cyclododecane, 2,2,9,11-tetramethylspiro[5.5]undec-8-en-1-yl acetate, 1-(octahydro-2,3,8,8-tetramethyl-2-naphthalenyl)-1-ethanone, Patchouli Oil, terpene fraction of patchouli oil, clearwood®, (1'R,E)-2-ethyl-4-(2',2',3'-trimethyl-3'-cyclopenten-1'-yl)-2-buten-1-ol, 2-ethyl-4-(2,2,3-trimethyl-3-cyclopenten-1-yl)-2-buten-1-ol, methyl cedryl ketone, 5-(2,2,3-trimethyl-3-cyclopentenyl)-3-methylpentan-2-ol, 1-(2,3,8,8-tetramethyl-1,2,3,4,6,7,8,8a-octahydronaphthalen-2-yl)ethan-1-one and / or isobornyl acetate; other ingredients (e.g. amber, powdery spicy or watery): dodecahydro-3a,6,6,9a-tetramethyl-naphtho[2,1-b]furan and any of its stereoisomers, heliotropin, anisaldehyde, eugenol, cinnamaldehyde, clove oil, 3-(1,3-benzodioxol-5-yl)-2-methylpropanal, 7-methyl-2H-1,5-benzodioxepin-3(4H)-one, 2,5,5-trimethyl-1,2,3,4,4a,5,6,7-octahydro-2-naphthalenol, 1-phenylvinyl acetate, 6-methyl-7-oxa-1-thia-4-azaspiro[4.4]nonane and / or 3-(3-isopropyl-1-phenyl)butanal.

[0054] It is also understood that the ingredient may be a compound known to release various types of perfuming compounds in a controlled manner, also known as a properfume or profragrance. Non-limiting examples of suitable pro-perfumes include 4-(dodecylthio)-4-(2,6,6-trimethyl-2-cyclohexen-1-yl)-2-butanone, 4-(dodecylthio)-4-(2,6,6-trimethyl-1-cyclohexen-1-yl)-2-butanone, trans-3-(dodecylthio)-1-(2,6,6-trimethyl-3-cyclohexen-1-yl)-1-butanone, 2-(dodecylthio)octan-4-one, 2-phenylethyloxo- (phenyl)acetate, 3,7-dimethylocta-2,6-dien-1-yloxo(phenyl)acetate, (Z)-hex-3-en-1-yloxo(phenyl)acetate, 3,7-dimethyl-2,6-octadien-1-ylhexadecanoate, bis(3,7-dimethylocta-2,6-dien-1-yl)succinate, (2-((2-methylundec-1-en-1-yl)oxy)ethyl)benzene, 1-methoxy-4-(3 -Methyl-4-phenethoxybut-3-en-1-yl)benzene, (3-methyl-4-phenethoxybut-3-en-1-yl)benzene, 1-(((Z)-hex-3-en-1-yl)oxy)-2-methylundec-1-ene, (2-((2-methylundec-1-en-1-yl)oxy)ethoxy)benzene, 2-methyl-1-(octan-3-yloxy)undec-1-ene, 1-methoxy-4-(1-phenethoxyprop-1- The methyl 4-(1-phenethoxyprop-1-en-2-yl)benzene may comprise 1-methyl-4-(1-phenethoxyprop-1-en-2-yl)benzene, 2-(1-phenethoxyprop-1-en-2-yl)naphthalene, (2-phenethoxyvinyl)benzene, 2-(1-((3,7-dimethyloct-6-en-1-yl)oxy)prop-1-en-2-yl)naphthalene, (2-((2-pentylcyclopentylidene)methoxy)ethyl)benzene or mixtures thereof.

[0055] The perfuming ingredients may be dissolved in a solvent currently used in the perfume industry. The solvent is preferably not alcohol. Examples of such solvents are diethyl phthalate, isopropyl myristate, Abalyn® (rosin resin, available from Eastman), benzyl benzoate, ethyl citrate, limonene or other terpenes, or isoparaffins. Preferably, the solvent is very hydrophobic and highly sterically hindered, such as Abalyn® or benzyl benzoate. Preferably, the perfume contains less than 30% solvent. More preferably, the perfume contains less than 20%, and even more preferably less than 10%, of solvent, all these percentages being defined by weight relative to the total weight of the perfume. More preferably, the perfume is substantially solvent-free.

[0056] Preferred perfuming ingredients are those with high steric hindrance and in particular those from one of the following groups: - Group 1: Perfuming ingredients containing a cyclohexane ring, a cyclohexene ring, a cyclohexanone ring or a cyclohexenone ring substituted with at least one linear or branched C1-C4 alkyl or alkenyl substituent; - Group 2: Perfuming ingredients containing a cyclopentane, cyclopentene, cyclopentanone or cyclopentenone ring substituted with at least one linear or branched C4-C8 alkyl or alkenyl substituent; - Group 3: perfuming ingredients containing a phenyl ring or a cyclohexane, cyclohexene, cyclohexanone or cyclohexenone ring substituted with at least one linear or branched C5-C8 alkyl or alkenyl substituent or with at least one phenyl substituent and optionally one or more linear or branched C1-C3 alkyl or alkenyl substituents; - Group 4: perfuming ingredients containing at least two fused or linked C5 and / or C6 rings; - Group 5: Perfuming ingredients containing ring structures such as camphor; - Group 6: At least one C7-C 20 Perfuming ingredients containing ring structures; - Group 7: Perfuming ingredients with a logP value above 3.5 and containing at least one tert-butyl or at least one trichloromethyl substituent. Examples of components from each of the above groups are: - Group 1: 2,4-dimethyl-3-cyclohexene-1-carbaldehyde (Supplier: Firmenich SA, Geneva, Switzerland), isocyclocitral, menthone, isomenthone, methyl 2,2-dimethyl-6-methylene-1-cyclohexanecarboxylate (Supplier: Firmenich SA, Geneva, Switzerland), nerone, terpineol, dihydroterpineol, terpenyl acetate, dihydroterpenyl acetate, dipentene, eucalyptol, hexylate, rose oxide, (S)-1,8-p-menthadien-7-ol (Supplier: Firmenich SA, Geneva, Switzerland), 1-p-menthen-4-ol, (1RS,3RS,4SR)-3-p-menthanyl acetate, (1R,2S,4R)-4,6,6-trimethyl-bicyclo[3,1,1]heptan-2-ol, tetrahydro-4-methyl-2-phenyl-2H-pyran (Supplier: Firmenich SA, Geneva, Switzerland), cyclohexyl acetate, cyclanol acetate, 1,4-cyclohexanediethyl dicarboxylate (Supplier: Firmenich SA, Geneva, Switzerland), (3ARS,6SR,7ASR)-perhydro-3,6-dimethyl-benzo[B]furan-2-one (Supplier: Firmenich SA, Geneva, Switzerland), (6R)-perhydro-3,6-dimethyl-benzo[B]furan-2-one (Supplier: Firmenich SA, Geneva, Switzerland), 2,4,6-trimethyl-4-phenyl-1,3-dioxane, 2,4,6-trimethyl-3-cyclohexene-1-carbaldehyde; - group 2: (E)-3-methyl-5-(2,2,3-trimethyl-3-cyclopenten-1-yl)-4-penten-2-ol (supplier: Givaudan SA, Vernier, Switzerland), (1'R,E)-2-ethyl-4-(2',2',3'-trimethyl-3'-cyclopenten-1'-yl)-2-buten-1-ol (supplier: Firmenich SA, Geneva, Switzerland), (1'R,E)-3,3-dimethyl-5-(2',2',3'-trimethyl-3'-cyclopenten-1'-yl)-4-penten-2-ol (supplier: Firmenich SA, Geneva, Switzerland), 2-heptylcyclopentanone, methyl-cis-3-oxo-2-pentyl-1-cyclopentaneacetate (supplier: Firmenich SA, Geneva, Switzerland), 2,2,5-trimethyl-5-pentyl-1-cyclopentanone (Supplier: Firmenich SA, Geneva, Switzerland), 3,3-dimethyl-5-(2,2,3-trimethyl-3-cyclopenten-1-yl)-4-penten-2-ol (Supplier: Firmenich SA, Geneva, Switzerland), 3-methyl-5-(2,2,3-trimethyl-3-cyclopenten-1-yl)-2-pentanol (Supplier: Givaudan SA, Vernier, Switzerland); - group 3: damascone, 1-(5,5-dimethyl-1-cyclohexen-1-yl)-4-penten-1-one (supplier: Firmenich SA, Geneva, Switzerland), nectalactone ((1'R)-2-[2-(4'-methyl-3'-cyclohexen-1'-yl)propyl]cyclopentanone), alpha-ionone, beta-ionone, damascenone, a mixture of 1-(5,5-dimethyl-1-cyclohexen-1-yl)-4-penten-1-one and 1-(3,3-dimethyl-1-cyclohexen-1-yl)-4-penten-1-one (supplier: Firmenich SA, Geneva, Switzerland), 1-(2,6,6-trimethyl-1-cyclohexen-1-yl)-2-buten-1-one (supplier: Firmenich SA, Geneva, Switzerland), (1S,1'R)-[1-(3',3'-dimethyl-1'-cyclohexyl)ethoxycarbonyl]methylpropanoate (Supplier: Firmenich SA, Geneva, Switzerland), 2-tert-butyl-1-cyclohexyl acetate (Supplier: International Flavors and Fragrances, USA), 1-(2,2,3,6-tetramethyl-cyclohexyl)-3-hexanol (Supplier: Firmenich SA, Geneva, Switzerland), trans-1-(2,2,6-trimethyl-1-cyclohexyl)-3-hexanol (Supplier: Firmenich SA, Geneva, Switzerland), (E)-3-methyl-4-(2,6,6-trimethyl-2-cyclohexen-1-yl)-3-buten-2-one, terpenyl isobutyrate, 4-(1,1-dimethylethyl)-1-cyclohexyl acetate (Supplier: Firmenich SA, Geneva, Switzerland), 8-methoxy-1-p-menthene, (1S,1'R)-2-[1-(3',3'-Dimethyl-1'-cyclohexyl)ethoxy]-2-methylpropylpropanoate (Supplier: Firmenich SA, Geneva, Switzerland), para-tert-butylcyclohexanone, menthenethiol, 1-methyl-4-(4-methyl-3-pentenyl)-3-cyclohexene-1-carbaldehyde, allyl cyclohexylpropionate, cyclohexyl salicylate, 2-methoxy-4-methylphenylmethyl carbonate, ethyl 2-methoxy-4-methylphenylcarbonate, 4-ethyl-2-methoxyphenylmethyl carbonate; Group 4: methyl cedryl ketone (supplied by: International Flavors and Fragrances, USA), a mixture of (1RS,2SR,6RS,7RS,8SR)-tricyclo[5.2.1.0~2,6~]dec-3-en-8-yl 2-methylpropanoate and (1RS,2SR,6RS,7RS,8SR)-tricyclo[5.2.1.0~2,6~]dec-4-en-8-yl 2-methylpropanoate, vetiverol, vetiveron, 1-(octahydro-2,3,8,8-tetramethyl-2-naphthalenyl)-1-ethanone (supplied by: International Flavors and Fragrances, USA). Fragrances, USA), (5RS,9RS,10SR)-2,6,9,10-tetramethyl-1-oxaspiro[4.5]deca-3,6-diene and (5RS,9SR,10RS) isomers, 6-ethyl-2,10,10-trimethyl-1-oxaspiro[4.5]deca-3,6-diene, 1,2,3,5,6,7-hexahydro-1,1,2,3,3-pentamethyl-4-indenone (Supplier: International Flavors and Fragrances, USA), a mixture of 3-(3,3-dimethyl-5-indanyl)propanal and 3-(1,1-dimethyl-5-indanyl)propanal (Supplier: Firmenich SA, Geneva, Switzerland), 3',4-dimethyl-tricyclo[6.2.1.0(2,7)]undec-4-ene-9-spiro-2'-oxirane (Supplier: Firmenich SA, Geneva, Switzerland), 9 / 10-ethyldiene-3-oxatricyclo[6.2.1.0(2,7)]undecane, perhydro-5,5,8A-trimethyl-2-naphthalenyl acetate (Supplier: Firmenich SA, Geneva, Switzerland), octalinol, dodecahydro-3a,6,6,9a-tetramethyl-naphtho[2,1-b]furan (Supplier: Firmenich SA, Geneva, Switzerland), tricyclo[5.2.1.0(2,6)]dec-3-en-8-yl acetate and tricyclo[5.2.1.0(2,6)]dec-4-en-8-yl acetate and tricyclo[5.2.1.0(2,6)]dec-3-en-8-ylpropanoate and tricyclo[5.2.1.0(2,6)]dec-4-en-8-ylpropanoate, (+)-(1S,2S,3S)-2,6,6-trimethyl-bicyclo[3.1.1]heptane-3-spiro-2'-cyclohexen-4'-one;. - Group 5: camphor, borneol, isobornyl acetate, 8-isopropyl-6-methyl-bicyclo[2.2.2]oct-5-ene-2-carbaldehyde, pinene, camphene, β-methoxycedrane, (8-methoxy-2,6,6,8-tetramethyl-tricyclo[5.3.1.0(1,5)]undecane (Supplier: Firmenich SA, Geneva, Switzerland), cedrene, cedrenol, cedrol, mixture of 9-ethylidene-3-oxatricyclo[6.2.1.0(2,7)]undecan-4-one and 10-ethylidene-3-oxatricyclo[6.2.1.0(2,7)]undecan-4-one (Supplier: Firmenich SA, Geneva, Switzerland), 3-methoxy-7,7-dimethyl-10-methylene-bicyclo[4.3.1]decane (Supplier: Firmenich SA, Geneva, Switzerland); - group 6: (trimethyl-13-oxabicyclo-[10.1.0]-trideca-4,8-diene (supplier: Firmenich SA, Geneva, Switzerland), Ambrettolide LG ((E)-9-hexadecen-16-olide, supplier: Firmenich SA, Geneva, Switzerland), pentadecenolide (supplier: Firmenich SA, Geneva, Switzerland), mucenone (3-methyl-(4 / 5)-cyclopentadecenone, supplier: Firmenich SA, Geneva, Switzerland), 3-methylcyclopentadecanone (supplier: Firmenich SA, Geneva, Switzerland), pentadecanolide (supplier: Firmenich SA, Geneva, Switzerland), cyclopentadecanone (supplier: Firmenich SA, Geneva, Switzerland), (1-ethoxyethoxy)cyclododecane (Supplier: Firmenich SA, Geneva, Switzerland), 1,4-dioxacycloheptadecane-5,17-dione, 4,8-cyclododecadien-1-one; - Group 7: (+-)-2-methyl-3-[4-(2-methyl-2-propanyl)phenyl]propanal (supplier: Givaudan SA, Vernier, Switzerland), 2,2,2-trichloro-1-phenylethyl acetate.

[0057] Preferably, the perfume comprises at least 30%, preferably at least 50%, more preferably at least 60% of ingredients selected from groups 1 to 7 as defined above. More preferably, the perfume comprises at least 30%, preferably at least 50%, of ingredients from groups 3 to 7 as defined above. Most preferably, the perfume comprises at least 30%, preferably at least 50%, of ingredients from groups 3, 4, 6 or 7 as defined above.

[0058] According to another preferred embodiment, said perfume comprises at least 30%, preferably at least 50%, more preferably at least 60% of ingredients with a logP greater than 3, preferably greater than 3.5, even more preferably greater than 3.75.

[0059] Preferably, the perfume used in the present invention contains less than 10% by weight of primary alcohols, less than 15% by weight of secondary alcohols, and less than 20% by weight of tertiary alcohols. Advantageously, the perfume used in the present invention does not contain any primary alcohols and contains less than 15% of secondary and tertiary alcohols.

[0060] According to one embodiment, the oil phase (or oily core) comprises: - 25-100% by weight of perfume oil containing at least 15% by weight of impact-resistant perfume raw materials having a Log T<-4, and - 1.07g / cm 3 Density-balanced materials with a density of 0 to 75% by mass Includes.

[0061] High impact perfume ingredients with Log T<-4 and 1.07g / cm 3 The properties of density-balanced materials having densities above 1000 .mu.m are described in WO 2018115250, the contents of which are incorporated by reference.

[0062] According to a particular embodiment, the hydrophobic material does not have any active ingredients (e.g., fragrances). In this particular embodiment, the hydrophobic material preferably comprises a hydrophobic solvent, preferably selected from the group consisting of isopropyl myristate, triglycerides (e.g., Neobee® MCT oil, vegetable oil), D-limonene, silicone oil, mineral oil, and mixtures thereof, and optionally 1,4-butanediol, benzyl alcohol, triethyl citrate, triacetin, benzyl acetate, ethyl acetate, propylene glycol (1,2-propanediol), 1,3-propanediol, dipropylene glycol, glycerol, glycol ethers, and mixtures thereof.

[0063] The term "biocide" refers to a chemical substance that can kill living organisms (e.g., microorganisms) or reduce or prevent their growth and / or accumulation. Biocides are commonly used in medicine, agriculture, forestry, and in industries to prevent fouling of, for example, water, agricultural products including seeds, and oil pipelines. Biocides may be pesticides, including fungicides, herbicides, insecticides, algicides, molluscicides, acaricides, and rodenticides; and / or antimicrobial agents, such as bactericides, antibiotics, antibacterial agents, antivirals, antifungals, antiprotozoals, and / or antiparasitics.

[0064] As used herein, "pesticide" refers to a substance that serves to repel or attract pests, reduce, inhibit, or promote their growth, development, or their activity. A pest refers to any organism, whether animal, plant, or fungus, that is invasive or bothersome to plants or animals; pests include insects, especially arthropods, mites, spiders, fungi, weeds, bacteria, and other microorganisms.

[0065] Terms such as "flavor ingredient" or "flavor" are understood to define various flavor and fragrance materials of both natural and synthetic origin, including single compounds or mixtures. Specific examples of such ingredients can be found in the literature, for example, Fenaroli's Handbook of Flavor Ingredients, 1975, CRC Press; Synthetic Food Adjuncts, 1947 by MB Jacobs, edited by van Nostrand; or Perfume and Flavor Chemicals by S. Arctander, 1969, Montclair, NJ (USA). These substances are well known to those skilled in the art of flavoring and / or perfuming foods and consumer products.

[0066] The flavoring ingredient may be a taste modifier. A "taste modifier" is understood as an active ingredient that acts on the consumer's taste receptors or provides sensory properties related to mouthfeel (e.g., body, roundness, or mouth-coating) to the consumed product. Non-limiting examples of taste modifiers include active ingredients that enhance, modify, or impart saltiness, fattiness, umami, kokumi, hot or cool, sweet, sour, tingly, bitter, or sour flavors.

[0067] The flavoring component may be a complex flavor that matches a particular organoleptic characteristic, for example, sweet and savory notes such as chicken, beef, pork, or shrimp flavor.

[0068] The core material may be in a liquid or solid state at a temperature between 20°C and 30°C.

[0069] According to one embodiment, the core material is liquid at a temperature between 20°C and 30°C.

[0070] According to another embodiment, the core material is solid at a temperature between 20°C and 30°C.

[0071] The core material may be hydrophobic, meaning that it is immiscible with water at temperatures between 20°C and 30°C and exists in the form of a separate hydrophobic phase.

[0072] The core may comprise at least 5% by weight, more preferably at least 10% by weight, even more preferably at least 20% by weight, most preferably at least 30% by weight, for example 40% by weight, of compounds having a vapor pressure above 0.007 Pa (vapor pressure is defined relative to a reference temperature of 25°C).

[0073] Preferably, at least 10% by weight of the core material has a vapour pressure above 0.1 Pa, more preferably at least 10% by weight has a vapour pressure >1 Pa at 25°C, and most preferably at least 10% by weight has a vapour pressure >10 Pa at 25°C.

[0074] A given value of 0.007 Pa at 25° C. for vapor pressure is generally considered the limit for identifying compounds with volatility. For the purposes of the present invention, vapor pressure is determined by calculation using the method disclosed in the "EPI Suite" software (2000, U.S. Environmental Protection Agency).

[0075] Preferably, the core of the coacervate core-shell microcapsules comprises a flavor ingredient, in other words, the flavor ingredient is encapsulated in the core of the coacervate core-shell microcapsules.

[0076] The core of the coacervate core-shell microcapsules may comprise a fatty matrix, preferably the fatty matrix comprises a food-grade oil.

[0077] The fat matrix may comprise (i) hydrogenated oil or (ii) hydrogenated fat or (iii) cocoa butter or (iv) a mixture of i-iii.

[0078] Preferably, the hydrogenated oils include hydrogenated palm oil, hydrogenated soybean oil, and hydrogenated cottonseed oil.

[0079] Preferably, the hydrogenated fat comprises cocoa fat.

[0080] More preferably, the fat matrix comprises a mixture of fat and hydrogenated oil, even more preferably, the fat matrix comprises a mixture of hydrogenated palm oil and coco fat and / or cocoa butter.

[0081] According to certain embodiments, the shell of the microcapsules further comprises an additional polymeric material, preferably selected from the group consisting of polyurea, polyurethane, polyamide, polyester, polyacrylate, polysiloxane, polycarbonate, polysulfonamide, polymers of urea and formaldehyde, melamine and formaldehyde, melamine and urea, or melamine and glyoxal, and mixtures thereof.

[0082] According to one embodiment, the shell is a composite shell formed from a coacervate material and a polymeric material.

[0083] According to certain embodiments, the additional polymeric material forms an inner layer.

[0084] According to one embodiment, the microcapsules comprise an inner shell made from a polymeric material and an outer coacervate shell comprising a plant protein extract.

[0085] The shell of the microcapsule may be crosslinked using a crosslinking agent. The shell of the capsule may be crosslinked using a crosslinking agent. Typically, a crosslinking agent is used to harden the microcapsule shell.

[0086] Cross-linking agents may include formaldehyde, tannins (eg, polyphenols), acetaldehyde, glutaraldehyde, glyoxal, chrome alum, or transglutaminase.

[0087] Preferably, the cross-linking agent is glutaraldehyde, which is well described in the public domain and is commercially available.

[0088] Preferably, the crosslinking is carried out at a temperature in the range of 5 to 40°C, preferably 15 to 25°C, more preferably 20 to 25°C.

[0089] Preferably, the pH during cross-linking is adjusted to a level at which cross-linking can be carried out effectively. Preferably, when cross-linking is carried out enzymatically using transglutaminase, the pH may be adjusted to 3 to 7, more preferably 3.5 to 5.5.

[0090] Preferably, crosslinking is carried out for a period of 1 to 15 hours, preferably 2 to 12 hours, more preferably 7 to 10 hours, in particular at ambient temperature (ie 20 to 25° C.).

[0091] Alternatively, crosslinking is carried out for a period of 1 to 15 hours, preferably 1 to 4 hours, especially at ambient temperature (ie 20 to 25° C.).

[0092] Alternatively, the shell may be hardened by other methods other than cross-linking using the aforementioned cross-linking agents. Such methods include: (i) hardening the shell by thermal annealing, achieved by heating the capsules; the heating is preferably carried out at a temperature close to the denaturation temperature of the protein, and most preferably at or above the denaturation temperature of the protein; (ii) hardening the shell by changing the pH to a range in which the shell density increases (which may be called a "pH quench"); (iii) hardening the shell by changing the ionic strength to a range in which the shell density increases, which may be achieved by adding a solute, preferably a salt; (iv) hardening the shell by modifying the continuous aqueous phase by adding a water-miscible additive, such that the shell density increases, preferably by adding glycerol, propylene glycol, ethanol, or isopropanol; and (v) hardening the shell by sequentially, simultaneously, any combination of methods i to iv, or by combining any of methods i to iv sequentially and simultaneously.

[0093] According to a particular embodiment, the shell is crosslinked solely by heat treatment.

[0094] Although the above alternative methods for hardening the shell are normally considered to be destructive and degradative procedures for the protein itself, it has surprisingly been found that such procedures result in a denser and more robust capsule shell because the protein is transformed from its native state into a denatured or coagulated state.

[0095] Another object of the present invention is a method for preparing coacervate core-shell microcapsules as defined above, comprising the steps of: a) preparing a hydrocolloid solution by dissolving at least one plant protein extract in an aqueous solution, preferably water; b) optionally preparing a hydrocolloid solution by dissolving at least one non-protein polymer in an aqueous solution, preferably water; c) optionally mixing a hydrocolloid solution comprising at least one plant protein extract and at least one non-protein polymer; d) preparing an emulsion and / or suspension by emulsifying and / or suspending a hydrophobic substance in a solution; e) forming a colloidal wall comprising the plant protein extract and, optionally, a non-protein polymer around the hydrophobic material present in the emulsion and / or suspension; and f) optionally cross-linking the colloidal wall The manufacturing method includes:

[0096] It is understood that any of steps c through e may be performed sequentially or simultaneously.

[0097] In other embodiments, any one or more of the process steps c, d and e described above may further comprise a dilution step in which additional solvent, preferably water, is added to the solution or any mixture thereof.

[0098] In certain alternative embodiments, the process step e) described above further comprises altering the pH value of the mixture.

[0099] In a preferred embodiment, process step e) of forming the colloidal wall comprises coacervation of a plant protein extract and optionally a non-protein polymer.

[0100] It is understood that the order of the process steps is a preferred order, but it may be possible to change the order of some steps. In certain alternative embodiments, step c) may be performed after step d), meaning that the hydrophobic substance is first emulsified in the solution prepared in step a) and the solution prepared in step b) is added only after emulsification.

[0101] According to one embodiment, the mass ratio between plant protein extract and non-protein polymer is between 1 and 100, in particular between 1 and 10, more in particular between 1 and 5.

[0102] According to a particular embodiment, the plant protein extract is obtained by acidic extraction of pulse flour (or pulse seeds or root vegetables), typically carried out at a pH of 2 to 5, preferably 2.5 to 3.5.

[0103] Typically, extraction is carried out by dispersing the pulse flour (or pulse seeds or root vegetables) in water, adjusting the pH value to a range of 1.5 to 5.5, preferably 2.5 to 3.5, using an acid, preferably hydrochloric acid, centrifuging the dispersion, and collecting the extract, which is present as a protein-rich supernatant.

[0104] According to another particular embodiment, the plant protein extract is obtained by basic extraction of pulse flour (or pulse seeds or root vegetables), typically carried out at a pH of 7-10, preferably 7.5-9.

[0105] The first solution comprises dissolving at least one plant extract in an aqueous solution, preferably water, and maintaining it at a temperature of 30°C to 50°C, preferably 35°C to 45°C, and even more preferably 38°C to 42°C.

[0106] In the first solution, the protein may be present in aqueous solution in an amount of 0.5 to 20% by weight, more preferably 1 to 15% by weight, even more preferably 7 to 13% by weight.

[0107] The second solution comprises dissolving at least a non-protein polymer, preferably gum arabic, in an aqueous solution, preferably water, and maintaining it at a temperature of 30°C to 50°C, preferably 35°C to 45°C, and even more preferably 38°C to 42°C.

[0108] In the second solution, the non-protein polymer may be present in the aqueous solution in an amount of 0.5 to 20% by weight, more preferably 1 to 15% by weight, and even more preferably 7 to 13% by weight.

[0109] The first solution and the second solution may be mixed under agitation to form a third solution.

[0110] The pH of the third solution may be adjusted to a pH value of less than 4.7, preferably less than 4.3, and most preferably less than 3.5.

[0111] The pH of the third aqueous solution may be adjusted by the addition of a food-grade acid solution, preferably by the addition of an aqueous lactic acid solution.

[0112] The hydrophobic material may be introduced into the third solution under shear to form an emulsion or suspension.

[0113] The emulsion or suspension may be prepared in a conventional manner.

[0114] The emulsion or suspension may be prepared by adding the hydrophobic material to the third solution over a period of about 3 to 10 minutes, preferably 4 to 6 minutes.

[0115] The emulsion or suspension may be prepared with a moving blade agitator adjusted to a speed of 300-400 rpm. The agitator speed may be adjusted as needed.

[0116] In this step, also known as the "coacervation" step, two separate phases may be created: a coacervate phase (rich in polymer) and a cosolvent (depleted in polymer). The coacervate phase may generally be composed of plant protein extract and, optionally, non-protein polymers.

[0117] Coacervation may be enhanced by altering the pH, preferably so that it approaches the isoelectric point of the protein, preferably by starting at a pH value below the isoelectric point and then increasing the pH.

[0118] When a non-protein polymer is present, the pH for the coacervation is preferably adjusted so that the positive charge of the protein is neutralized by the negative charge of the non-protein polymer.

[0119] The pH is adjusted by the addition of a food-grade acid or base solution, preferably aqueous lactic acid and sodium hydroxide solution.

[0120] Phase separation can also be induced in a variety of other ways, such as by changing the physicochemical environment of the solution, typically at pH 2-5, by salting out or adding a second high molecular weight component to induce phase separation.

[0121] According to certain embodiments, when the core-shell microcapsules comprise additional polymeric material, a multifunctional monomer is added to the oil phase (in addition to the hydrophobic material) and / or the water phase.

[0122] "Multifunctional monomer" means a molecule that, as a unit, chemically reacts or combines to form a polymer or supramolecular polymer. The multifunctional polymer of the present invention has at least two functional groups capable of forming a microcapsule shell.

[0123] The multifunctional monomer may be selected from the group consisting of at least one polyisocyanate, polymaleic anhydride, polyacid chloride, polyepoxide, acrylate monomer, polyalkoxysilane, melamine-based resin, and mixtures thereof.

[0124] According to a particular embodiment, the polyfunctional monomer used in the process according to the invention is present in an amount corresponding to 0.1 to 15% by weight of the oil or aqueous phase, preferably 0.5 to 10% by weight, more preferably 0.8 to 6% by weight, even more preferably 1 to 3% by weight.

[0125] According to a particular embodiment, the monomer added in step a) is at least one polyisocyanate having at least two isocyanate functional groups.

[0126] Suitable polyisocyanates for use in accordance with the present invention include aromatic polyisocyanates, aliphatic polyisocyanates, and mixtures thereof. The polyisocyanates contain at least two, preferably at least three, but up to six, or even only four, isocyanate functional groups. In certain embodiments, triisocyanates (three isocyanate functional groups) are used.

[0127] According to one embodiment, said polyisocyanate is an aromatic polyisocyanate.

[0128] The term "aromatic polyisocyanate" as used herein is meant to encompass any polyisocyanate containing an aromatic moiety. Preferably, the aromatic moiety is a phenyl, toluoyl, xylyl, naphthyl, or diphenyl moiety, more preferably a toluoyl or xylyl moiety. Preferred aromatic polyisocyanates are biurets, polyisocyanurates, and trimethylolpropane adducts of diisocyanurates, more preferably containing one of the above-mentioned specific aromatic moieties. More preferably, the aromatic polyisocyanate is polyisocyanurate of toluene diisocyanate (commercially available from Bayer under the trade name Desmodur® RC), trimethylolpropane adduct of toluene diisocyanate (commercially available from Bayer under the trade name Desmodur® L75), or trimethylolpropane adduct of xylylene diisocyanate (commercially available from Mitsui Chemicals under the trade name Takenate® D-110N). In a most preferred embodiment, the aromatic polyisocyanate is a trimethylolpropane adduct of xylene diisocyanate.

[0129] According to another embodiment, the polyisocyanate is an aliphatic polyisocyanate. The term "aliphatic polyisocyanate" is defined as a polyisocyanate that does not contain any aromatic moieties. Preferred aliphatic polyisocyanates are the trimer of hexamethylene diisocyanate, the trimer of isophorone diisocyanate, the trimethylolpropane adduct of hexamethylene diisocyanate (commercially available from Mitsui Chemicals) or the biuret of hexamethylene diisocyanate (commercially available from Bayer under the trade name Desmodur® N 100), with the biuret of hexamethylene diisocyanate being even more preferred.

[0130] According to another embodiment, the at least one polyisocyanate is in the form of a mixture of at least one aliphatic polyisocyanate and at least one aromatic polyisocyanate, both containing at least two or three isocyanate functional groups, such as a mixture of a biuret of hexamethylene diisocyanate and a trimethylolpropane adduct of xylene diisocyanate, a mixture of a biuret of hexamethylene diisocyanate and a polyisocyanurate of toluene diisocyanate, and a mixture of a biuret of hexamethylene diisocyanate and a trimethylolpropane adduct of toluene diisocyanate. Most preferred is a mixture of a biuret of hexamethylene diisocyanate and a trimethylolpropane adduct of xylene diisocyanate. Preferably, when used as a mixture, the molar ratio of aliphatic polyisocyanate to aromatic polyisocyanate is in the range of 80:20 to 10:90.

[0131] Another object of the present invention are coacervate core-shell microcapsules obtainable by the process defined above.

[0132] Another object of the present invention is a method for producing a microcapsule powder, comprising the steps defined above and an additional step consisting of subjecting the slurry obtained in step e) or f) to drying, such as spray drying, to provide the microcapsules themselves, i.e., in powder form. It is understood that any standard method known to those skilled in the art for carrying out such drying can be applied. In particular, the slurry may be spray-dried, preferably in the presence of a polymeric carrier material, such as polyvinyl acetate, polyvinyl alcohol, dextrin, natural or modified starch, vegetable gum, pectin, xanthan, alginate, carrageenan, or a cellulose derivative, to provide the microcapsules in powder form.

[0133] However, other drying methods may also be mentioned, such as extrusion, plating, spray granulation, fluidized bed or drying at room temperature using materials (carriers, desiccants) that meet the specific criteria disclosed in WO 2017 / 134179.

[0134] According to certain embodiments, the carrier material comprises free hydrophobic material which may be the same or different from the hydrophobic material from the core of the microcapsule.

[0135] consumer products By "consumer product" or "finished product" we mean a manufactured product that is prepared for distribution, sale and use by a consumer.

[0136] The microcapsules of the invention can be used for the preparation of perfuming or flavoring compositions, which are also the object of the present invention.

[0137] Flavored Consumer Products The powder compositions of the present invention can be used in a wide variety of edible end products.

[0138] The final product is more particularly a food, pet food or feed product. Because the microcapsules of the present invention are plant-based, they are particularly advantageous for vegetarian meat or meat substitutes, vegetarian burgers, sausages, patties, chicken imitation nuggets... meat products (e.g. processed meat, poultry, beef, pork, ham, fresh sausage or cured meat preparations, spiced or marinated fresh meat or cured meat products, modified meats), or extended meat products using combinations of animal protein and plant protein in various ratios, often mixing plant protein and animal protein that are co-extruded or interwoven.

[0139] Meat for the purposes of the present invention includes meat such as beef, pork, sheep, lamb, game and poultry such as chicken, turkey, goose and duck. Preferably, the food product of the present invention is meat selected from beef, chicken and pork.

[0140] In one aspect, the flavored consumer product is selected from the group consisting of protein powders, protein drinks, protein bars, meat analogs, seafood analogs, and savory goods.

[0141] Meat analogs may include pork analog, venison analog, beef analog, veal analog, rabbit analog, sausage analog, deli meat analog, ham analog, salami analog, pepperoni analog, chicken analog, turkey analog, goose analog, pheasant analog, pigeon analog, whale analog, lamb analog, goat analog, donkey analog, squirrel analog.

[0142] Seafood analogs can include fish analogs, scallop analogs, shrimp analogs, crab meat analogs, crustacean analogs, clam analogs, squid analogs, snail analogs, and ascidian analogs.

[0143] When the flavored consumer product is a particulate or powdered food product, the dry particles may be easily added thereto by dry mixing. Typical flavored articles are selected from the group consisting of instant soups or sauces, breakfast cereals, powdered milk, baby food, powdered beverages, powdered chocolate beverages, spreads, powdered cereal beverages, chewing gum, effervescent tablets, cereal bars, and chocolate bars. The powdered food or beverage may be intended to be consumed after reconstituting the product with water, milk and / or juice, or other aqueous liquids.

[0144] The dry particles provided herein may be suitable for providing flavor to beverages, liquid dairy products, condiments, baked goods, frostings, bakery fillings, candies, chewing gum and other food products.

[0145] Beverages include, but are not limited to, carbonated soft drinks, including cola, lemon-lime, root beer, heavy citrus ("dew-type"), fruit flavors, and cream soda; powdered soft drinks, and liquid concentrates, such as fountain syrups and cordials; coffee and coffee-based drinks, coffee substitutes, and cereal-based drinks; tea, including dry mix products and ready-to-drink teas (herbal and tea leaf-based); fruit and vegetable juices and juice-flavored beverages, as well as juice drinks, nectars, concentrates, punches, and "ades"; carbonated and still sweetened and flavored waters; sports / energy / health drinks; alcoholic beverages, including beer and malt beverages, cider, and wine (still, sparkling, fortified, and wine coolers), and alcohol-free and other low-alcohol products; other beverages processed by heating (infusion, heat pasteurization, ultra-high temperature, ohmic heating, or industrial aseptic sterilization) and hot-fill packaging; and cool-fill products produced through filtration or other preservation techniques.

[0146] Liquid dairy products include, but are not limited to, unfrozen, partially frozen, and frozen liquid dairy products such as milk, ice cream, sorbet, and yogurt.

[0147] Condiments include, but are not limited to, ketchup, mayonnaise, salad dressing, Worcestershire sauce, fruit flavored sauce, chocolate sauce, tomato sauce, chili sauce, and mustard.

[0148] Baked products include, but are not limited to, cakes, cookies, pastries, breads, donuts, and the like.

[0149] Bakery fillings include, but are not limited to, low or neutral pH fillings, high, medium or low solids fillings, fruit or milk-based (pudding-type or mousse-type) fillings, hot or cold fillings, and non-fat to full-fat fillings.

[0150] Nevertheless, the microcapsules of the present invention may be of particular interest in the following product examples: - Baked goods (e.g. bread, dry biscuits, cakes and other baked goods), - Non-alcoholic drinks (e.g. carbonated drinks, mineral water, sports / energy drinks, juice drinks, vegetable juices, vegetable juice preparations), - alcoholic beverages (e.g. beer and malt drinks, spirit drinks), - instant drinks (e.g. instant vegetable drinks, powdered soft drinks, instant coffee and tea), - Cereal products (e.g. breakfast cereals, cooked ready-made rice products, rice flour products, millet and sorghum products, raw or cooked noodle and pasta products), - dairy products (e.g. fresh cheese, soft cheese, hard cheese, milk drinks, whey, butter, partially or totally hydrolyzed milk protein-containing products, fermented milk products, condensed milk and the like); - Dairy-based products (e.g. fruit yogurt, flavored yogurt, ice cream, fruit ice cream), - confectionery products (e.g. chewing gum, hard and soft candies), - Chocolate and compound coatings, - Products based on fats and oils or their emulsions (e.g. mayonnaise, spreads, margarines, shortenings, remoulades, dressings, spice products), - Spiced, marinated or processed fish products (e.g. fish sausages, minced fish), - Eggs or egg products (dried eggs, egg whites, egg yolks, custard), desserts (e.g. gelatin and puddings), - products made from soy protein or other soy ingredients (e.g. soy milk and products made therefrom, soy lecithin-containing products, fermented products such as tofu or tempeh or products made therefrom, soy sauce); - vegetable products (e.g. ketchup, sauces, processed and reconstituted vegetables, dried vegetables, frozen vegetables, cooked vegetables, pickled vegetables, vegetable concentrates or pastes, cooked vegetables, potato products), - Vegetarian meat substitutes or meat alternatives, vegetarian burgers spices or spice products (for example mustard products, horseradish products), spice mixtures and in particular seasonings used, for example, in the snack sector; snack products (e.g. baked or fried potato chips or potato dough products, bread dough products, corn, rice or peanut based extrudates), - meat products (e.g. processed meat, poultry, beef, pork, ham, fresh sausage or fresh meat preparations, spiced or marinated fresh or cured meat products, modified meats); - Ready-to-eat meals (e.g., instant noodles, rice, pasta, pizza, tortillas, wraps) and soups and stocks (e.g., stocks, savory cubes, dry soups, instant soups, cooked soups, retort soups), sauces (instant sauces, dry sauces, ready-made sauces, gravies, sweet sauces).

[0151] Preferably, the microcapsules according to the present invention are used in products selected from the group consisting of baked goods, instant drinks, cereal products, dairy products, products based on fats or oils or emulsions thereof, desserts, vegetable preparations, vegetarian meat substitutes, spices and seasonings, snacks, meat products, ready meals, soups and bouillons and sauces.

[0152] According to certain embodiments, the flavored product is selected from the group consisting of meat and / or fish based foods or analogs, soups, savory cubes, powder mixes, beef or pork based products, seafood, surimi, instant noodles, rice, soups, sauces, prepared meals, frozen or chilled pizza, pasta, potato flakes or fries, noodles, potato / tortilla chips, microwave popcorn, nuts, pretzels, mochi, rice crackers, fermented milk analog beverages, acidified dairy analog beverages, non-fermented dairy analog beverages, cheese or cheese analogs, yogurt or yogurt analogs, dietary supplements, nutritional bars, cereals, ice cream, non-dairy ice cream, confectionery products, chewing gum, hard boiled candy, and powdered beverages.

[0153] According to one embodiment, the food, pet food or feed product comprises 0.01 to 10% by weight, preferably 0.1 to 5% by weight, of the microcapsules of the invention.

[0154] Typically, the food, pet food or feed product further comprises protein, especially vegetable protein or animal protein, and mixtures thereof.

[0155] Advantageously, the vegetable protein is preferably chosen from soy protein, corn, pea, canola, sunflower, sorghum, rice, amaranth, potato, tapioca, arrowroot, chickpea, lupin, canola, wheat, oats, rye, barley, and mixtures thereof.

[0156] The microcapsules of the present invention are particularly suitable for extruded and / or baked food, pet food, or feed products, more specifically extruded and / or baked food, pet food, or feed products containing animal and / or vegetable proteins. Typically, the extruded and / or baked food, pet food, or feed product may be selected from meat-based and / or fish-based foods or analogs and mixtures thereof (i.e., meat-based foods and / or fish-based foods or meat substitutes or fish analogs and mixtures thereof), with extruded and / or baked meat substitutes or extruded and / or baked fish analogs being preferred. Non-limiting examples of extruded and / or baked food, pet food, or feed products include snack products or extruded vegetable proteins intended to incorporate proteins to form meat substitutes (e.g., hamburgers). The powder composition can be added to either non-extruded vegetable protein isolates / concentrates or interwoven vegetable proteins forming hamburgers or nuggets (or the like) before or after extrusion.

[0157] flavored consumer products The microcapsules of the present invention can be used in combination with an active ingredient. (i) microcapsules as defined above; (ii) an active ingredient, preferably selected from the group consisting of a cosmetic ingredient, a skin care ingredient, a fragrance ingredient, a flavor ingredient, a malodor-neutralizing ingredient, a bactericide ingredient, a fungicide ingredient, a pharmaceutical or pesticide ingredient, a disinfectant ingredient, an insect repellent or attractant, and mixtures thereof; A composition comprising:

[0158] The microcapsules of the present invention may be used in different perfumed consumer products.

[0159] In particular, a perfuming composition comprising (i) microcapsules as defined above, (ii) at least one perfuming co-ingredient, and (iii) optionally a perfuming adjuvant is another object of the present invention.

[0160] Furthermore, the term "perfuming co-ingredient" refers herein to a compound that is used in a perfuming preparation or composition to impart a pleasant effect and is not a microcapsule as defined above. In other words, to be considered a perfuming co-ingredient, it must be recognized by those skilled in the art that such a co-ingredient can impart or modify the odor of the composition in a positive or desirable way, and does not simply have one odor. The nature and type of perfuming co-ingredient present in the perfuming composition, which will in any case not be exhaustive, do not warrant a more detailed description here, and those skilled in the art can select the base based on their general knowledge and according to the intended use or application and the desired organoleptic effect. In general terms, these perfuming co-ingredients belong to various chemical classes, such as alcohols, lactones, aldehydes, ketones, esters, ethers, acetates, nitriles, terpenoids, nitrogen or sulfur heterocyclic compounds, and essential oils, and the perfuming co-ingredients may be of natural or synthetic origin. Many of these auxiliary ingredients are in any case mentioned in references, for example in S. Arctander's "Perfume and Flavor Chemicals," 1969, Montclair, New Jersey, USA, or its latest editions, or in other works of a similar nature, as well as in the abundant patent literature in the field of perfumery. It is also understood that said auxiliary ingredients may be compounds known to release various types of perfuming compounds (e.g. pro-perfumes) in a controlled manner.Non-limiting examples of suitable pro-perfumes include 4-(dodecylthio)-4-(2,6,6-trimethyl-2-cyclohexen-1-yl)-2-butanone, 4-(dodecylthio)-4-(2,6,6-trimethyl-1-cyclohexen-1-yl)-2-butanone, trans-3-(dodecylthio)-1-(2,6,6-trimethyl-3-cyclohexen-1-yl)-1-butanone, 2-(dodecylthio)octan-4-one, 2-phenylethyloxo (phenyl)acetate, 3,7-dimethylocta-2,6-dien-1-yloxo(phenyl)acetate, (Z)-hex-3-en-1-yloxo(phenyl)acetate, 3,7-dimethyl-2,6-octadien-1-ylhexadecanoate, bis(3,7-dimethylocta-2,6-dien-1-yl)succinate, (2-((2-methylundec-1-en-1-yl)oxy)ethyl)benzene, 1-methoxy-4-(3 -Methyl-4-phenethoxybut-3-en-1-yl)benzene, (3-methyl-4-phenethoxybut-3-en-1-yl)benzene, 1-(((Z)-hex-3-en-1-yl)oxy)-2-methylundec-1-ene, (2-((2-methylundec-1-en-1-yl)oxy)ethoxy)benzene, 2-methyl-1-(octan-3-yloxy)undec-1-ene, 1-methoxy-4-(1-phenethoxyprop-1- The methyl 4-(1-phenethoxyprop-1-en-2-yl)benzene may comprise 1-methyl-4-(1-phenethoxyprop-1-en-2-yl)benzene, 2-(1-phenethoxyprop-1-en-2-yl)naphthalene, (2-phenethoxyvinyl)benzene, 2-(1-((3,7-dimethyloct-6-en-1-yl)oxy)prop-1-en-2-yl)naphthalene, (2-((2-pentylcyclopentylidene)methoxy)ethyl)benzene or mixtures thereof.

[0161] By "perfuming adjuvants" is meant here ingredients that can impart additional benefits, such as color, particular light resistance, chemical stability, etc. A detailed description of the nature and type of adjuvants customarily used in perfuming bases cannot be exhaustive, but it should be mentioned that said ingredients are well known to those skilled in the art.

[0162] Preferably, the perfuming composition according to the invention comprises 0.1 to 30% by weight of microcapsules as defined above.

[0163] The microcapsules of the present invention can be advantageously used in many fields of application and can be used in consumer products: the microcapsules can be used in liquid form for liquid consumer products and in powder form for powder consumer products.

[0164] In the case of microcapsules containing a perfume oil-based core, the products of the present invention can be used in particular in perfumed consumer products, such as those belonging to refined fragrances or "functional" perfumery. Functional perfumery includes in particular personal care products, including hair care, body cleansing, skin care, and hygiene care, as well as home care products, including laundry care and air care. Therefore, another object of the present invention consists in perfumed consumer products containing, as perfuming ingredients, microcapsules as defined above or a perfume composition as defined above. The perfume elements of said consumer products may be combinations of perfume microcapsules as defined above and free or unencapsulated perfumes, as well as other types of perfume microcapsules other than those disclosed herein.

[0165] In particular, - 2 to 65% by weight, relative to the total weight of the consumer product, of at least one surfactant, - water or a water-miscible hydrophilic organic solvent, and - a perfuming composition or microcapsule as defined above, wherein the hydrophobic material comprises a perfume A liquid consumer product containing the compound is another object of the present invention.

[0166] below, - 2 to 65% by weight, based on the total weight of the consumer product, of at least one surfactant, and - a perfuming composition or microcapsule as defined above, wherein the hydrophobic material comprises a perfume Powdered consumer products containing the compound are also part of the present invention.

[0167] According to certain embodiments, the method for preparing microcapsules for inclusion in perfumed consumer products comprises a cross-linking step (chemical and / or enzymatic) as defined above to improve stability in difficult substrates containing high amounts of surfactants.

[0168] The microcapsules of the present invention may therefore be added as such or as part of the perfuming composition of the present invention in perfumed consumer products.

[0169] For reasons of clarity, it should be noted that by "perfumed consumer product" it is meant a consumer product that is expected to provide, among other benefits, a perfuming effect to the surface to which the perfuming effect is applied (e.g. skin, hair, fabrics, paper or household surfaces) or in the air (air freshener, deodorizer, etc.) In other words, a perfumed consumer product according to the invention is a product that comprises a functional formulation, also referred to as a "base", together with an active substance in an effective amount of the microcapsules according to the invention.

[0170] The nature and type of other constituents of the perfumed consumer product do not warrant a more detailed description here, which would in any case not be exhaustive, and those skilled in the art can select the nature and type of ingredients of the perfumed consumer product based on their general knowledge and according to the nature and desired effect of the product. The base formulations of consumer products that can be incorporated into the microcapsules of the present invention can be found in the abundant literature on such products. These formulations do not warrant a detailed description here and are not in every case exhaustive. Those skilled in the art of preparing such consumer products can completely select suitable ingredients based on their general knowledge and the available literature.

[0171] Non-limiting examples of suitable perfumed consumer products include perfumes, such as fine perfumes, colognes, aftershave lotions, body splashes; fabric care products, such as liquid or solid detergents, tablets and pods, fabric softeners, dryer sheets, fabric refreshers, ironing water, or bleach; personal care products, such as hair care products (e.g., shampoos, hair conditioners, hair dyes, or hair sprays), cosmetic formulations (e.g., vanishing creams, body lotions, or deodorants or antiperspirants), or skin care products (e.g., clothing It may be a scented soap, shower or bath smoothie, body wash, oil or gel, bath salts or hygiene product; an air care product such as an air freshener or a "ready to use" powder air freshener; or a home care product such as an all purpose cleanser, a liquid or powder or tablet dishwashing product, a toilet cleanser or a product for cleaning various surfaces such as sprays and wipes intended to treat / refresh textiles or hard surfaces (floors, tiles, stone floors, etc.); a hygiene product such as a sanitary napkin, diaper, toilet paper.

[0172] Another object of the present invention is to provide - personal care active bases, and - a microcapsule as defined above or a perfuming composition as defined above wherein the consumer product is in the form of a personal care composition.

[0173] The personal care active bases that can be incorporated into the microcapsules of the present invention can be found in the extensive literature on such products. Their preparation does not warrant a detailed description herein and is not exhaustive in every case. Those skilled in the art of preparing such consumer products are entirely capable of selecting suitable ingredients based on their general knowledge and the available literature.

[0174] The personal care composition is preferably selected in the group consisting of a hair care product (e.g. shampoo, hair conditioner, hair dye or hair spray), a cosmetic formulation (e.g. vanishing cream, body lotion or deodorant or antiperspirant), or a skin care product (e.g. perfumed soap, shower or bath mousse, body wash, oil or gel, bath salts, or hygiene product), an oral care product (toothpaste or mouthwash composition) or a refined fragrance product (e.g. Eau de Toilette-EdT).

[0175] Another object of the present invention is to provide - a home care or fabric care active base, and - a microcapsule as defined above or a perfuming composition as defined above wherein the consumer product is in the form of a home care or fabric care composition.

[0176] Home care or fabric care active bases that can be incorporated into the microcapsules of the present invention can be found in the extensive literature on such products. Their preparation does not warrant a detailed description herein and is not exhaustive in every case. Those skilled in the art of preparing such consumer products are entirely able to select suitable ingredients based on their general knowledge and the available literature.

[0177] The home care or fabric care composition is preferably selected in the group consisting of fabric softeners, liquid detergents, powder detergents, liquid scent boosters, solid fragrances.

[0178] For the liquid consumer products referred to below, reference to an "active base" should be understood to include an active agent (typically including a surfactant) and water.

[0179] For the solid consumer products referred to below, reference to an "active base" should be understood to include active materials (typically including surfactants) and optionally adjuvants (e.g., bleaches, buffers, builders, soil-releasing or soil-suspending polymers, granular enzyme particles, corrosion inhibitors, defoamers, suds suppressors, dyes, fillers, and mixtures thereof).

[0180] Fabric softener The object of the present invention is to provide the following: - a fabric softener active base, preferably comprising at least one active material selected from the group consisting of dialkyl quaternary ammonium salts, dialkyl ester quaternary ammonium salts (esterquats), Hamburg esterquats (HEQ), TEAQ (triethanolamine quats), silicones and mixtures thereof, the active base being used in an amount preferably between 85 and 99.95% by weight relative to the total weight of the composition; a microcapsule slurry as defined above, preferably in an amount ranging from 0.05 to 15% by weight, more preferably from 0.1 to 5% by weight, relative to the total weight of the composition; - preferably free perfume oil, and a consumer product in the form of a fabric softener composition comprising:

[0181] Liquid detergent The object of the present invention is to provide the following: - a liquid detergent active base, preferably comprising at least one active material selected from the group consisting of anionic surfactants, such as alkylbenzenesulfonates (ABS), secondary alkylsulfonates (SAS), primary alcohol sulfates (PAS), lauryl ether sulfates (LES), methyl ester sulfonates (MES), and nonionic surfactants, such as alkylamines, alkanolamides, fatty alcohol poly(ethylene glycol) ethers, fatty alcohol ethoxylates (FAE), ethylene oxide (EO) and propylene oxide (PO) copolymers, amine oxides, alkyl polyglucosides, alkyl polyglucosamides, the active base being used in an amount preferably between 85 and 99.95% by weight relative to the total weight of the composition; a microcapsule slurry as defined above, preferably in an amount ranging from 0.05 to 15% by weight, more preferably from 0.1 to 5% by weight, relative to the total weight of the composition; - Optional free perfume oil and a consumer product in the form of a liquid detergent composition comprising:

[0182] Solid detergent The object of the present invention is to provide the following: - a solid detergent active base, preferably comprising at least one active material selected from the group consisting of anionic surfactants, such as alkylbenzenesulfonates (ABS), secondary alkylsulfonates (SAS), primary alcohol sulfates (PAS), lauryl ether sulfates (LES), methyl ester sulfonates (MES), and nonionic surfactants, such as alkylamines, alkanolamides, fatty alcohol poly(ethylene glycol) ethers, fatty alcohol ethoxylates (FAE), ethylene oxide (EO) and propylene oxide (PO) copolymers, amine oxides, alkyl polyglucosides, alkyl polyglucosamides, wherein the active base is used in an amount preferably between 85 and 99.95% by weight relative to the total weight of the composition. a microcapsule powder or a microcapsule slurry as defined above, preferably in an amount ranging from 0.05 to 15% by weight, more preferably from 0.1 to 5% by weight, relative to the total weight of the composition; - Optional free perfume oil and a consumer product in the form of a liquid detergent composition comprising:

[0183] Shampoo / Shower gel The object of the present invention is to provide the following: - a shampoo or shower gel active base, preferably comprising at least one active material selected from the group consisting of sodium alkyl ether sulfates, ammonium alkyl ether sulfates, alkyl amphoacetates, cocamidopropyl betaine, cocamide MEA, alkyl glucosides and amino acid-based surfactants, and mixtures thereof, the active base being used in an amount preferably ranging from 85 to 99.95% by weight relative to the total weight of the composition; a microcapsule slurry as defined above, preferably in an amount ranging from 0.05 to 15% by weight, more preferably from 0.1 to 5% by weight, relative to the total weight of the composition; - optionally, free perfume oil and a consumer product in the form of a shampoo or shower gel composition comprising:

[0184] Rinse-off conditioner The object of the present invention is to provide the following: - a rinse-off conditioner active base, preferably comprising at least one active material selected from the group consisting of cetyltrimonium chloride, stearyltrimonium chloride, benzalkonium chloride, behentrimonium chloride and mixtures thereof, the active base being used in an amount preferably ranging from 85 to 99.95% by weight relative to the total weight of the composition; a microcapsule slurry as defined above, preferably in an amount ranging from 0.05 to 15% by weight, more preferably from 0.1 to 5% by weight, relative to the total weight of the composition; - Optional free perfume oil and a rinse-off conditioner composition comprising:

[0185] Solid scent booster The object of the present invention is to provide the following: solid carriers, preferably selected from the group consisting of urea, sodium chloride, sodium sulfate, sodium acetate, zeolites, sodium carbonate, sodium bicarbonate, clays, talc, calcium carbonate, magnesium sulfate, gypsum, calcium sulfate, magnesium oxide, zinc oxide, titanium dioxide, calcium chloride, potassium chloride, magnesium chloride, zinc chloride, sugars such as sucrose, mono-, di- and polysaccharides, and derivatives such as starch, cellulose, methylcellulose, ethylcellulose, propylcellulose, polyols / sugar alcohols such as sorbitol, maltitol, xylitol, erythritol and isomalt, PEG, PVP, citric acid or any water-soluble solid acid, fatty alcohol or fatty acid, and mixtures thereof, a microcapsule slurry as defined above, in powder form, preferably in an amount ranging from 0.05 to 15% by weight, more preferably from 0.1 to 5% by weight, relative to the total weight of the composition; - optionally, free perfume oil and a consumer product in the form of a solid fragrance composition comprising:

[0186] Liquid scent booster The object of the present invention is to - aqueous phase, - a surfactant system consisting essentially of one or more nonionic surfactants, wherein the surfactant system has an average HLB of 10 to 14 and is preferably selected from the group consisting of ethoxylated fatty alcohols, POE / PPG (polyoxyethylene and polyoxypropylene) ethers, monoglyceryl esters, polyglyceryl esters, sucrose ester compounds, polyoxyethylene hydroxyl esters, alkyl polyglucosides, amine oxides and combinations thereof; a linker selected from the group consisting of alcohols, salts and esters of carboxylic acids, salts and esters of hydroxyl carboxylic acids, fatty acids, fatty acid salts, glycerol fatty acids, surfactants with an HLB of less than 10, and mixtures thereof; - a microcapsule slurry as defined above, in the form of a slurry, in an amount preferably ranging from 0.05 to 15% by weight, more preferably from 0.1 to 5% by weight, relative to the total weight of the composition; - optionally, free perfume oil and a consumer product in the form of a liquid flavoring composition comprising:

[0187] hair dye The object of the present invention is to provide the following: an oxidation phase comprising an oxidizing agent, and an alkaline phase comprising an alkynyl agent, a dye precursor, and a coupling compound, in an amount preferably ranging from 85 to 99.95% by weight relative to the total weight of the composition, wherein said dye precursor and said coupling compound form an oxidative hair dye in the presence of the oxidizing agent; microcapsules as defined above, preferably in an amount ranging from 0.05 to 15% by weight, more preferably from 0.1 to 5% by weight, relative to the total weight of the composition; - optionally, free perfume oil and a consumer product in the form of a hair dye composition comprising:

[0188] Perfuming composition According to a particular embodiment, the consumer product comprises, based on the total weight of the perfuming composition: - 0.1 to 30% by weight, preferably 0.1 to 20% by weight, of microcapsules as defined above, 0 to 40% by weight, preferably 3 to 40% by weight, of a fragrance, and 20 to 90% by mass of ethanol, preferably 40 to 90% by mass The perfume composition is in the form of a perfume composition comprising:

[0189] The present invention will now be further illustrated by the following examples, with the understanding that the invention as claimed is in no way limited by these examples.

[0190] Example 1 Preparation of core-shell microcapsules according to the present invention A mixture of 10% (w / w) defatted soy flour and 15 Mm sodium metabisulfite was prepared in 100 mL of deionized water. The pH was adjusted to 2.7 with 1 M HCl and the mixture was stirred for 1 hour.

[0191] The pH rose slightly to 2.9 and was readjusted back to 2.8.

[0192] The resulting mixture was centrifuged at 10000 g for 30 min, and the supernatant (designated "Solution 1" for the remainder of this example) was used for further processing steps.

[0193] A 1 ml aliquot of the supernatant was used to measure the remaining mass fraction of protein using thermogravimetric analysis.

[0194] Separately, a solution of gum arabic (Efficacia®, Nexira, "Solution 2") is prepared at a concentration of 10% (w / w) or 15% (w / w).

[0195] 100 g of solution 1 and 100 g of solution 2 are added under mechanical shear to 340 g of warm demineralized water, the pH is readjusted to 2.7 using 1 M HCl and the mixture is kept at 30° C. for 15 minutes.

[0196] A 60 g quantity of the flavor composition to be encapsulated (limonene oil) was slowly added to the mixture and homogenized at 230 RPM for 5 minutes to reach an average droplet size of 150 μm as determined by optical microscopy. The stirring speed was slightly reduced, and 0.102 g of glutaraldehyde (50% by weight aqueous solution, supplied by Sigma-Aldrich) was added to the mixture. The microcapsule suspension was mixed for 4 to 10 hours at 20 to 25°C.

[0197] The result was an aqueous suspension of microcapsules or a slurry (see Figure 1).

[0198] Example 2 Comparative Example Using Soy Protein Isolate As a comparative example, the encapsulation experiment described in Example 1 was carried out, but instead of using the protein extract described in this invention, a standard commercially available soy protein isolate (SPI) was used. A solution of SPI (Dupont, "Solution 1") was prepared in demineralized water at a concentration of 10% (w / w). Separately, a solution of gum arabic (Efficacia®, Nexira, "Solution 2") was prepared at a concentration of 10% (w / w) or 15% (w / w).

[0199] 100 g of solution 1 and 100 g of solution 2 were added to 340 g of warm demineralized water under mechanical shear and the pH was readjusted to 2.7 using 1 M HCl. The mixture was kept at 30° C. for 14 minutes.

[0200] A 60 g quantity of the flavor composition to be encapsulated (limonene oil) was slowly added to the mixture and homogenized at 230 RPM for 5 minutes to reach an average droplet size of 150 μm as confirmed by optical microscopy. The stirring speed was slightly reduced, and 0.102 g of glutaraldehyde (50% by weight aqueous solution, supplied by Sigma-Aldrich) was added to the mixture. The microcapsule suspension was mixed for 4 to 10 hours at 20 to 25 °C.

[0201] Unlike Example 1, in this case no microcapsules were obtained, but instead an emulsion of unencapsulated oil droplets and loose particles.

[0202] Example 3 Preparation of core-shell microcapsules according to the invention using pea flour and gum arabic A mixture of 10% (w / w) pea flour (AM Nutrition, 14% protein) and 0.285 g of sodium metabisulfite (15 mM) was prepared in 100 mL of deionized water. The pH was adjusted to 2.7 with 1 M HCl, and the mixture was stirred for 1 h. The pH rose slightly to approximately 2.9 and was readjusted to 2.8. The resulting mixture was centrifuged at 10,000 g for 30 min, and the supernatant containing soluble pea protein and carbohydrates was collected. The supernatant was then lyophilized for storage. 100 mL of the supernatant contained 1.675 g of dry polymer containing protein and carbohydrate.

[0203] A solution was prepared by mixing a pea polymer solution and a gum arabic (Sigma-Aldrich) solution. 10 mL of the pea polymer solution (pH 3, 0.02 g / mL) was mixed with 10 mL of a gum arabic solution (pH 3) (pea polymer:gum arabic = 1:0.3). The mixture was added to warm deionized water under mechanical shear, the pH was adjusted to 2.7 using 1 M HCl, and the mixture was maintained at 30 °C for 15 min.

[0204] The flavor composition to be encapsulated (limonene oil) was slowly added to the mixture and homogenized at 230 RPM for 5 minutes. The stirring speed was slightly reduced and glutaraldehyde (50% by weight aqueous solution, supplied by Sigma-Aldrich) was added to the mixture. The microcapsule suspension was mixed for 4-10 hours at 20-25°C.

[0205] Example 4 Preparation of core-shell microcapsules according to the invention using pea flour or soy protein extract and chitosan Alkaline extraction of proteins from soybean and pea flour was carried out as in Examples 1 and 3, but at pH 8. The pH was then changed to pH 5.8 and mixed with oppositely charged chitosan. The chitosan was presolubilized at a pH of approximately 2-3 and returned to pH 5.8 before use. The flavor composition to be encapsulated (limonene oil) was slowly added to the mixture and homogenized at 230 RPM for 5 minutes. The stirring speed was slightly reduced, and glutaraldehyde (50% by weight aqueous solution, supplied by Sigma-Aldrich) was added to the mixture. The microcapsule suspension was mixed for 4-10 hours at 20-25°C.

[0206] Colloidal wall materials were formed by complex coacervation using similar protein and polysaccharide concentrations as in Examples 1 and 3.

[0207] Example 5 Characterization by SDS-PAGE of plant proteins and the complex coacervates obtained therefrom extracted with gum arabic.

[0208] The protein extracts obtained from soybean and pea flours, as well as the resulting complex coacervates with gum arabic and the coacervates prepared according to Examples 1 and 3, were further analyzed by SDS-PAGE to obtain detailed information on the protein composition of both the extracts and the coacervates. Methods and reference data for proteins from soybeans and peas are described in publications by Lam, A.C.Y. et al. ("Pea protein isolates: Structure, extraction, and functionality," Food Reviews International 34.2 (2018), pages 126-147; and Bogracheva, T.Y., N.Y., Bespalova, and A.L. Leont'ev. "Isolation of 11S and 7S globulins from seeds of glycine max." Applied Biochemistry and Microbiology 32.4 (1996), pages 429-433).

[0209] Globulins (glycinin and beta-conglycinin) account for 80% of soy protein. The fractions expected according to the two cited references are found in both the extract and the coacervate and contain smaller subunits of protein. These data not only confirm the quality of the protein extract obtained herein, but also clearly demonstrate that the complex coacervates obtained according to the present invention indeed contain the correct protein fractions of the corresponding proteins, providing confirmation of the composition of these complex coacervates.

[0210] Example 6 Cross-linking of coacervate capsules Capsules were prepared according to Example 1, and the resulting capsules were cross-linked. The capsule slurry obtained from Example 1 was further treated at 20°C by adding a cross-linking agent; glutaraldehyde solution (50% by weight aqueous solution, supplied by Sigma-Aldrich) was added to the mixture at a concentration of 0.068% (w / w). Cross-linking was carried out at 20°C for 4-10 hours.

[0211] Example 7 Hardening of the coacervates according to the invention by increasing the temperature.

[0212] Capsules were prepared according to Example 1 and hardened with an additional heating step, thereby densifying the coacervate shell. 300 ml of the capsule slurry prepared according to Example 1 was heated from 20°C to 80°C for 1 hour, held at 80°C for 5 minutes, and cooled to 20°C.

[0213] To mimic the conditions during this heating step and measure the rheological properties of the complex coacervate shell materials, oscillatory shear rheology (documented methods suitable for characterizing such properties, such as those summarized in the books "The Structure and Rheology Complex Fluids" (RG Larson, Oxford University Press, 1998) and "Understanding Rheology" (FA Morrison, Oxford University Press 2001)) were also used. The properties measured were the elastic modulus (also called "storage modulus" in some publications), which describes the elastic behavior of a material for a specific frequency and amplitude of deformation, conventionally written as G', and the viscous modulus (also called "loss modulus" in some publications), which describes the viscous behavior of a material for a specific frequency and amplitude of deformation, conventionally written as G", with G' and G" having units of Pascals (Pa).

[0214] Heat ramps were performed in a rheometer (Anton Paar MCR 501 with a 25 mm cone-and-plate geometry, Peltier temperature control, and a solvent trap to prevent water evaporation, as described in the book cited in the previous paragraph). Coacervate shell material, obtained from the complex coacervation of soy protein extract with gum arabic, was placed in the rheometer geometry, and the temperature was increased from 20 °C to 80 °C over 1 h, held at 80 °C for 5 min, and finally decreased again at a similar rate, as previously described for the capsule heating step. The results are shown in Figure 3 and clearly show that the complex coacervate shell material changes from a liquid-like substance (G″ > G′, meaning the viscous modulus is initially higher than the elastic modulus) to a solid-like substance (G′ > G″, meaning the elastic modulus becomes higher than the viscous modulus during heating) at a temperature of 50 °C, reaching a plateau near 70 °C with nearly constant G′ and G″. Upon cooling, the material always remains solid-like, and the elastic modulus G′ remains higher than the viscous modulus G″. The elastic modulus further increases during cooling, reaching values ​​of approximately 10°C. 5These data demonstrate that hardening by increasing temperature does indeed convert the original liquid coacervate into a solid shell.

[0215] Example 8 Production of food containing capsules prepared according to the present invention and comparison with comparative capsules Model flavors were encapsulated according to the present invention as described in Example 1, and for comparison, soy protein isolate (instead of the soy protein extract obtained by acidic extraction) was used as described in Example 2. 1 g of each of the resulting flavor slurries was stirred into a commercially available fermented milk analog product (soy milk-based yogurt) and allowed to stand in a refrigerator for 24 hours. Sensory evaluation revealed that the flavor was already strongly perceived olfactorily for the product flavored with the comparative flavor slurry from Example 2, even before the product was actually tasted. In contrast, the encapsulated flavor from Example 1 prepared according to the present invention was only perceived upon tasting the product, thereby confirming that these flavor capsules provide flavor protection and localization in complex product matrices such as dairy analogs.

[0216] Similarly, capsules prepared according to the invention from Example 1 were also compared to a comparative flavor slurry from Example 2 in a mayonnaise-type sauce (Thomy Mayonnaise Extra Light, purchased from a local supermarket) containing 1 g of flavor slurry according to Examples 1 or 2 per 100 g of product, using flavor sauce samples stored for 24 hours after preparation. As with the dairy analog evaluation, the flavor encapsulated according to the invention as in Example 1 was only significantly released upon tasting the product, thereby confirming successful encapsulation and release, while the flavor of Example 2 was already present throughout the product and exhibited a strong odor even before it was placed in the mouth.

Claims

1. Coacervate core-shell microcapsules containing a hydrophobic material. a) encapsulating a hydrophobic material in the core of a coacervate core-shell microcapsule; and b) the shell of the coacervate core-shell microcapsules comprises at least one plant protein extract and a non-protein polymer; The plant protein extract contains carbohydrates and / or lipids; Microcapsules, wherein the protein content in the plant protein extract is less than 55% by mass.

2. 2. The microcapsules of claim 1, wherein the plant protein extract is selected from the group consisting of protein extracts from soybean, pea, wheat, rice, potato, quinoa, amaranth, lentil, hemp, oat, buckwheat, chickpea, lupin seed, canola, linseed, and mixtures thereof.

3. 3. Microcapsules according to claim 1 or 2, wherein the non-protein polymer is selected from the group consisting of gum arabic, carboxymethylcellulose, chitosan, xanthan, agar, alginate, pectinate or carrageenan.

4. 4. Microcapsules according to any one of claims 1 to 3, wherein the plant protein extract is soy protein extract and the non-protein polymer is gum arabic.

5. 5. The microcapsules of claim 1, wherein the shell of the microcapsules is crosslinked using formaldehyde, tannin, acetaldehyde, glutaraldehyde, glyoxal, chrome alum, transglutaminase, and mixtures thereof.

6. 6. Microcapsules according to any one of claims 1 to 5, wherein the ratio of plant protein extract to non-protein polymer is from 1 to 100.

7. 7. A microcapsule according to any one of claims 1 to 6, wherein the shell comprises an additional polymeric material.

8. 8. The microcapsule of claim 7, wherein the polymeric material is selected from the group consisting of polyurea, polyurethane, polyamide, polyester, polyacrylate, polysiloxane, polycarbonate, polysulfonamide, polymers of urea and formaldehyde, melamine and formaldehyde, melamine and urea, or melamine and glyoxal, and mixtures thereof.

9. A method for producing coacervate core-shell microcapsules as defined in any one of claims 1 to 8, comprising the steps of: a) preparing a hydrocolloid solution by dissolving at least one plant protein extract in an aqueous solution; b) preparing a hydrocolloid solution by dissolving at least one non-protein polymer in an aqueous solution; c) mixing a hydrocolloid solution comprising at least one plant protein extract and at least one non-protein polymer; d) preparing an emulsion and / or suspension by emulsifying and / or suspending a hydrophobic substance in a solution; and e) forming a colloidal wall comprising the plant protein extract and the non-protein polymer around droplets and / or particles of hydrophobic material present in the emulsion and / or suspension; wherein the plant protein extract contains carbohydrates and / or lipids, and the protein content in the plant protein extract is less than 55% by mass.

10. 10. The method of claim 9, wherein the plant protein extract is obtained by acid or alkaline extraction of pulse flour, pulse seeds, or root vegetables.

11. 11. The method of claim 10, wherein the extraction is an acidic extraction.

12. 9. A consumer product comprising the coacervate core-shell microcapsules of any one of claims 1 to 8, wherein the consumer product is a flavored or fragranced product.

13. 13. The consumer product of claim 12, wherein the fragranced product is selected from the group consisting of a liquid or solid detergent, a fabric softener, a liquid or solid fragrance, a shampoo, a shower gel, a hair conditioning product, a deodorant, or an antiperspirant.

14. 13. The consumer product of claim 12, wherein the flavored product is selected from the group consisting of meat and / or fish based foods or analogs, soups, savory cubes, powder mixes, beef or pork based products, seafood, surimi, instant noodles, rice, soups, sauces, prepared meals, frozen or chilled pizza, pasta, potato flakes or fries, noodles, potato / tortilla chips, microwave popcorn, nuts, pretzels, mochi, rice crackers, fermented milk analog beverages, acidified dairy analog beverages, non-fermented dairy analog beverages, cheese or cheese analogs, yogurt or yogurt analogs, dietary supplements, nutritional bars, cereals, ice cream, non-dairy ice cream, confectionery products, chewing gum, hard boiled candy and powdered beverages.

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