Hair care composition and method for preparing same

Abstract

The present invention provides a hair care composition and a method for preparing the same. The hair care composition contains nicotinamide mononucleotide, a salt thereof, or a hydrate thereof, and a basic component that stabilizes nicotinamide mononucleotide, a salt thereof, or a hydrate thereof. The components of the hair care composition of the present invention increase the stability of nicotinamide mononucleotide in liquid form, solving problems related to hair and hair aging and growth, and providing a novel approach to the stability of nicotinamide mononucleotide in liquid form, opening up a breakthrough in diversifying the application methods of nicotinamide mononucleotide.

Description

[Technical Field]

[0001] The present invention is in the field of personal care, and specifically relates to hair care compositions containing nicotinamide mononucleotide, a salt thereof, or a hydrate thereof. [Background technology]

[0002] Recent biochemical research has shown that the level of coenzyme I in the human body gradually decreases with age. Related studies have also shown that a decrease in coenzyme I levels is closely related to diseases such as diabetes, which are caused by slowed metabolism and impaired function, and to the development of cancer, which is caused by the loss of cellular autorepair functions. Given these circumstances, supplementing the body's coenzyme I levels offers a direct and effective solution. Numerous scientific studies on the mechanism of coenzyme I increase, as well as animal and clinical pathology studies, have been conducted both domestically and internationally. All of these studies have consistently confirmed that coenzyme I supplementation effectively improves the health problems listed above. Furthermore, animal experiments have demonstrated that this method improves the athletic performance of elderly mice, while control mice show poorer athletic performance in old age.

[0003] Nicotinamide mononucleotide (NMN) is the most direct precursor converted to coenzyme I (nicotinamide adenine dinucleotide, NAD+) in the human body. Research has shown that nicotinamide mononucleotide most directly and rapidly increases coenzyme I levels in various organs and cells of the human body. Coenzyme I transports hydrogen ions (H-) primarily during biological reactions in various cells of the human body, and is an essential substance for biological reactions such as regulating metabolism, gene repair, and the physiological clock. Thus, intracellular coenzyme I levels are closely related to health.

[0004] Hair growth is also closely related to coenzyme I. Recent research projects on hair growth have shown that lactic acid synthesis and its levels are important basic elements in initiating the hair growth cycle within hair follicle cells, and that hair follicle cells require large amounts of ATP to maintain their normal function. Coenzyme I and its reduced form are important raw materials for synthesizing lactic acid in hair follicle cells. When the level of coenzyme I in cells decreases, lactic acid cannot be synthesized, and the level decreases, temporarily halting hair growth.

[0005] Coenzyme I is also an essential substrate for ATP synthesis in cellular mitochondria. A decrease in coenzyme I prevents hair follicle cell mitochondria from synthesizing sufficient ATP, making it difficult to support the cells' daily functions. When hair follicle cells simultaneously lack both lactic acid and ATP, hair growth gradually slows, eventually leading to hair loss. As hair follicles wither, pigment cells decrease, and hair turns gray. Both of these phenomena and the decline in physical function are the result of aging and are caused by a decrease in the body's coenzyme I. While the body's coenzyme I levels can be restored through exercise and controlled energy absorption, the effectiveness varies from person to person and cannot be achieved over a long period of time. Oral nicotinamide mononucleotide supplements offer a simple and direct method for effectively increasing the overall level of coenzyme I in the body. While oral nicotinamide mononucleotide supplements can significantly improve and promote various aspects of the body's health, they do not improve the appearance of scalp and other hair areas in the shortest possible time. To solve the above problems, the best approach is to develop a hair care composition with nicotinamide mononucleotide as the main active functional ingredient.

[0006] When using a hair care composition, it must be effectively and evenly distributed on the skin, so that nicotinamide mononucleotide, the main active ingredient of the care composition, can contact the epidermis and hair follicles and reach the cells.To achieve this goal, the hair care composition must be in a liquid form with a different viscosity.However, nicotinamide mononucleotide is less stable in liquid form, and the half-life of an aqueous solution is generally 14 days.Due to the instability of nicotinamide mononucleotide in liquid form, there are technical difficulties in the application of nicotinamide mononucleotide.This is also the main reason why related products cannot be produced and sold on the market. Summary of the Invention [Problem to be solved by the invention]

[0007] To solve at least one of the above problems, the present invention provides a hair care composition and a method for preparing the same. The components of the hair care composition of the present invention provide a solution for increasing the stability of nicotinamide mononucleotide in liquid form, which not only solves problems related to hair and hair aging and growth, but also shows a novel approach to the stability of nicotinamide mononucleotide in liquid form, and opens up a breakthrough in diversifying the application methods of nicotinamide mononucleotide. [Means for solving the problem]

[0008] Specifically, the present invention provides the following: A hair care composition comprising nicotinamide mononucleotide, a salt thereof, or a hydrate thereof, and a base ingredient that stabilizes nicotinamide mononucleotide, a salt thereof, or a hydrate thereof. Here, the nicotinamide mononucleotide, its salt, or its hydrate is in the form of a solid, liquid, or mist. Here, the basic components that stabilize nicotinamide mononucleotide, its salt, or its hydrate include one or more of anionic surfactants, amphoteric surfactants, cationic surfactants, antioxidants, pH buffers, and acidity adjusters.

[0009] Here, the hair care composition may be in the form of any one of shampoo, conditioner, essence, soap, spray, cream, hair wax, gel, and paste. Here, the product containing the hair care composition is any one of an eyebrow care product, an eyelash care product, and a beard care product. Here, the basic component contains one or more components selected from sialic acid, theanine, β-carotene, and sodium phosphate as components that prevent nicotinamide mononucleotide from being decomposed when it is in liquid or mist form.

[0010] Here, the nicotinamide mononucleotide, its salt, or its hydrate accounts for 0.01 wt % to 25 wt % of the total weight of the hair care composition, preferably 0.1 wt % to 20 wt % of the total weight of the hair care composition, and more preferably 0.1 wt % to 15 wt % of the total weight of the hair care composition, calculated in terms of nicotinamide mononucleotide. wherein the hair care composition further comprises water, Optionally, the water content is 50 to 95 wt %, preferably 60 to 92 wt %, more preferably 65 to 90 wt %, based on the total weight of the hair care composition.

[0011] wherein, based on the total weight of the hair care composition, The content of the anionic surfactant is 15 to 65% by weight, preferably 25 to 45% by weight, and more preferably 30 to 40% by weight. Optionally, the content of the amphoteric surfactant is 1 to 15% by weight, preferably 1.5 to 8% by weight, more preferably 2 to 7% by weight; Optionally, the content of the cationic surfactant is 0.01 to 0.09 wt %, preferably 0.02 to 0.08 wt %, more preferably 0.03 to 0.08 wt %, Optionally, the content of the antioxidant is 0.5 to 3 wt %, preferably 0.9 to 2.5 wt %, more preferably 1.5 to 2 wt %; Optionally, the content of the acidity regulator is 0.05 to 1% by weight, preferably 0.2 to 0.85% by weight, more preferably 0.3 to 0.7% by weight; Optionally, the content of the pH buffering agent is 1 to 3% by weight, preferably 1.5 to 2.5% by weight, and more preferably 2 to 2.5% by weight.

[0012] Here, the anionic surfactant is at least one selected from sodium laureth sulfate, sodium lauryl sulfate, ammonium lauryl sulfate, sodium methyl cocoyl taurate, laureth-5 carboxylic acid, sodium lauryl glucose carboxylate, and mixtures thereof. Here, the amphoteric surfactant is at least one of alkyl amphoteric carboxylate salt, alkyl amino acid salt, cocamidopropyl betaine, cocamide monoethanolamine, lauryl glucoside, cocoyl methyl dextran, or a mixture thereof. Here, the cationic surfactant is at least one selected from the group consisting of polyquaternium-10, guar hydroxypropyltrimethylammonium chloride, and mixtures thereof.

[0013] Here, the antioxidant is at least one selected from the group consisting of L-cysteine, DL-methionine, vitamin A, vitamin C, vitamin E, sodium phytate, and mixtures thereof. Here, the acidity regulator is at least one selected from citric acid, oxalic acid, lactic acid, hydrochloric acid, and mixtures thereof. Here, the eyebrow care products are eyebrow care lotions, eyebrow care sprays, and eyebrow care creams, the eyelash care products are eyelash care lotion kits, and the beard care products are beard care lotions, beard care sprays, and beard care creams.

[0014] Here, the hair care composition further contains functional additives to improve the appearance, texture, scent and user experience of the hair care composition. Here, the functional additive is selected from a sensitizer, a foam booster, a fragrance, a functional adjuvant, or any combination thereof. providing nicotinamide mononucleotide, a salt thereof, or a hydrate thereof; providing an aqueous solution of a base component that stabilizes nicotinamide mononucleotide, its salt, or its hydrate in liquid or gaseous form; and mixing nicotinamide mononucleotide, a salt thereof, or a hydrate thereof with the aqueous solution and adjusting the pH value to obtain a hair care composition. Here, examples of basic components that stabilize nicotinamide mononucleotide, its salt, or its hydrate in liquid or gaseous form include one or more of anionic surfactants, amphoteric surfactants, cationic surfactants, nonionic surfactants, antioxidants, pH buffers, and acidity adjusters.

[0015] where: (1) mixing one or more anionic surfactants to obtain a first class surfactant; (2) mixing one or more amphoteric surfactants to obtain a second class surfactant; (3) optionally, mixing one or more cationic surfactants to obtain a third class surfactant; (4) mixing a class 1 surfactant, a class 2 surfactant, and an optional class 3 surfactant to obtain a composite surfactant; (5) adding nicotinamide mononucleotide to the complex surfactant to obtain a mixture; (6) adding a pH buffer to the mixture and adjusting the pH value of the mixture to 4.85 to 7.0 using an acidity adjuster to obtain a hair care composition; (7) adding an antioxidant to the mixture to obtain a hair care composition. [Effects of the Invention]

[0016] The present invention has at least one of the following advantages and beneficial effects. 1. The present invention provides a hair care composition that contains nicotinamide mononucleotide as a major active ingredient and stably maintains the activity of nicotinamide mononucleotide, a salt thereof, or a hydrate thereof for a long period of time. The composition supplies nicotinamide mononucleotide to mitochondria in hair follicle stem cells and epidermal cells, thereby increasing the coenzyme I levels in the hair follicle stem cells and epidermal cells, promoting normal energy production in the cells, and activating their vitality. 2. Compared with nicotinic acid, nicotinamide, and other derivatives, nicotinamide mononucleotide in the present invention is the most direct precursor of coenzyme I. When absorbed into cells, it is directly converted into coenzyme I, and is not restricted by multiple enzymatic reactions in the body. In addition, nicotinamide mononucleotide is a small molecule that can be rapidly absorbed by the human body and reach epidermal cells. 3. The hair care composition of the present invention can directly increase the coenzyme I levels in hair follicle stem cells and epidermal cells. Compared with oral administration of nicotinamide mononucleotide supplements alone, the present invention can improve hair problems more significantly and achieve more pronounced results in a shorter period of time. Therefore, the present invention is suitable for middle-aged and elderly people who suffer from hair loss and gray hair problems, people with slow hair growth, and those with thinning hair, as well as the general public who are concerned about hair health. [Brief explanation of the drawings]

[0017] [Figure 1] FIG. 1 shows the comparative stability results over 25 days of Example 1, Example 15, Example 16 and a 0.5% aqueous solution of nicotinamide mononucleotide under normal conditions. [Figure 2] FIG. 2 shows the comparative stability results of Example 1, Example 15, Example 16 and 0.5% nicotinamide mononucleotide aqueous solution under accelerated conditions over 25 days. [Figure 3] 3(a) to (d) are photographs showing the hair growth status of a female subject who used the hair essence containing nicotinamide mononucleotide of Example 4 on days 0, 30, 60, and 120, respectively. [Figure 4] FIG. 4 shows the change over the number of days in the average hair spacing at the hairline of a female subject who used the hair essence containing nicotinamide mononucleotide of Example 4. DETAILED DESCRIPTION OF THE INVENTION

[0018] The present invention will be further described below with reference to specific embodiments, but these are not intended to limit the present invention. Those skilled in the art can make various changes or modifications based on the basic concept of the present invention, and any such changes or modifications are within the scope of the present invention as long as they do not deviate from the basic concept of the present invention.

[0019] Slowed hair growth, gradual graying, and hair loss are some of the most noticeable signs of aging and may be due to a lack of ATP supply to hair follicle cells. Hair follicle cells synthesize the energy molecule ATP through biochemical reactions such as glycolysis in the mitochondria, providing enough energy for hair growth and maintaining normal hair follicle function. Research has shown that low ATP levels cause hair follicle cells to gradually die, causing hair to stop growing, lose pigment cells, gradually turn gray, and then fall out and die. ATP synthesis in hair follicle cells differs from ATP synthesis in other cells. Most general body cells synthesize ATP using glucose, which requires less coenzyme I. Hair follicle cells rarely synthesize ATP this way, instead often using glutamine as a substrate, which doubles the amount of coenzyme I used in this synthesis pathway. For various reasons, coenzyme I levels in the body decline with age, and the coenzyme I levels in hair follicle cells also decline accordingly, making ATP synthesis more difficult and preventing normal cell function. Hair follicle cells are also activated by lactic acid, and lactate dehydrogenase, which expresses lactic acid in the cells, is the catalytic enzyme that synthesizes lactic acid from pyruvate using hydrogen ions provided by reduced coenzyme I. If there is a lack of lactic acid, hair follicle cells cannot grow new hair. The simultaneous occurrence of these two conditions is directly related to the decrease in intracellular coenzyme I levels.

[0020] Coenzyme I is an essential biochemical for maintaining hair follicle health and promoting hair growth. Therefore, increasing the body's coenzyme I levels is a fundamental solution to addressing slow hair growth, graying, and hair loss. While oral nicotinamide mononucleotide supplements effectively increase the overall body level of coenzyme I, they only provide a limited supply of the active ingredients required by hair and hair follicle cells, and their effects are not immediate. Therefore, they require long-term administration for their effects to be gradually seen.

[0021] To solve the above problems, the present invention provides a hair care composition containing nicotinamide mononucleotide. This composition can be applied directly to hair, scalp, epidermis, and hair follicles, and most effectively replenishes and increases the level of coenzyme I in hair follicles and epidermal cells to achieve the goals of increasing ATP synthesis and lactic acid proliferation, thereby maintaining the health of hair follicles and activating hair follicle cells to promote hair regrowth. This hair care composition can be used to alleviate, improve, and ultimately restore various scalp and hair problems in middle-aged and elderly people, maintaining hair health and promoting hair growth.

[0022] The hair care composition of the present invention is obtained by adding nicotinamide mononucleotide to specific basic ingredients for stabilization (e.g., specific surfactants, additives, antioxidants, etc., and their component ratios within specific ranges). When using a hair care product, the user directly applies it to the scalp, hair, and skin. Nicotinamide mononucleotide, the main active ingredient in the product, is absorbed into hair follicles and epidermal cells, where it is combined with ATP and coenzyme I synthesized in the mitochondria by nicotinamide mononucleotide adenyltransferase. This coenzyme I is involved in the biochemical synthesis of ATP and lactic acid in hair follicles and epidermal cells. Furthermore, after being reconverted to reduced coenzyme I during lactic acid synthesis, coenzyme I becomes a necessary coenzyme in the catalytic process of synthesizing lactic acid from pyruvate and acts as a carrier for hydrogen ions. The hair care composition of the present invention increases the level of coenzyme I in hair follicles and epidermal cells, thereby addressing the problems of graying and hair loss due to aging, maintaining hair health, and accelerating the hair growth cycle.

[0023] Nicotinamide mononucleotide further maintains excellent liquid stability in hair care compositions, allowing the hair care compositions of the present invention to maintain their activity better than common aqueous solutions of nicotinamide mononucleotide. The present invention promotes the usability and industrial applicability of nicotinamide mononucleotide in hair care compositions, resolves the long-standing problem of the difficulty of industrialized production due to the liquid stability of nicotinamide mononucleotide, and provides users with a fundamental and novel solution to hair problems based on biochemical scientific research.

[0024] In one aspect, the present invention provides a hair care composition containing nicotinamide mononucleotide, a salt thereof, or a hydrate thereof, and a basic component that stabilizes nicotinamide mononucleotide, a salt thereof, or a hydrate thereof. The basic component that stabilizes nicotinamide mononucleotide, a salt thereof, or a hydrate thereof includes one or more of anionic surfactants, amphoteric surfactants, cationic surfactants, neutral surfactants, antioxidants, pH buffers, and acidity adjusters. The care composition of the present invention can maintain the stability of nicotinamide mononucleotide, a salt thereof, or a hydrate thereof in an aqueous care composition for a long period of time, and as a result, the hair care composition containing nicotinamide mononucleotide, a salt thereof, or a hydrate thereof can maintain its effective quality for a long period of time.

[0025] Nicotinamide mononucleotide, its salt or its hydrate In each embodiment of the present invention, the hair care composition contains nicotinamide mononucleotide, a salt thereof, or a hydrate thereof. The physical form of nicotinamide mononucleotide, a salt thereof, or a hydrate thereof may be solid, liquid, or mist. The chemical structure of nicotinamide mononucleotide may be an acid form, a salt form, a crystal water form, a hydrate form, or another addition form. The purity of this physical form and chemical structure may be 5% to 99.8%, preferably 80% to 99.8%, and more preferably 97.5% to 99.5%. In a specific embodiment of the present invention, the content of the nicotinamide mononucleotide, its salt, or its hydrate is 0.01% by weight to 25% by weight, preferably 0.01% by weight to 20% by weight, and more preferably 0.1% by weight to 15% by weight, based on the total weight of the hair care composition.

[0026] Basic Ingredients One feature of the present invention is that the hair care composition contains a base component that stabilizes nicotinamide mononucleotide, its salt, or its hydrate, and the base component that stabilizes nicotinamide mononucleotide, its salt, or its hydrate may include one or more of anionic surfactants, amphoteric surfactants, cationic surfactants, neutral surfactants, antioxidants, pH buffers, and acidity adjusters.

[0027] Anionic surfactants In the hair care composition of the present invention, an anionic surfactant can also stabilize nicotinamide mononucleotide, its salt, or its hydrate. The anionic surfactant may be a fatty alcohol polyether sulfate, or may be ammonium laureth sulfate, sodium laureth sulfate, sodium lauryl sulfate, ammonium lauryl sulfate, sodium methyl cocoyl taurate, laureth-5 carboxylic acid, sodium lauryl glucose carboxylate, or the like, either singly or in combination. In a specific embodiment of the present invention, the content of the anionic surfactant is 15 to 65 wt %, preferably 25 to 45 wt %, more preferably 30 to 40 wt %, based on the total weight of the hair care composition.

[0028] amphoteric surfactants Such surfactants can exhibit both anionic and cationic properties and have excellent biocompatibility. In the hair care composition of the present invention, the amphoteric surfactant can be used not only to remove dirt but also to stabilize nicotinamide mononucleotide, its salt, or its hydrate. The amphoteric surfactant may be an alkylamino acid amphoteric surfactant or an alkyl amphoteric carboxylate salt. Examples of amphoteric surfactants include cocamidopropyl betaine, cocamide monoethanolamine, lauryl glucoside, and cocoyl methyl dextran, either singly or in combination. In a specific embodiment of the present invention, the content of the amphoteric surfactant is 1 to 15 wt %, preferably 1.5 to 8 wt %, more preferably 2 to 7 wt %, based on the total weight of the hair care composition. Other amphoteric surfactants include alkyl amphoteric carboxylic acids or salts thereof, such as alkyl amphoteric dipropionates, alkyl amphoteric diacetates, alkyl amphoteric glycinates, and alkali metal salts of alkyl amphoteric propionates, where the alkyl group is an alkyl group having 6 to 20 carbon atoms. More preferably, the alkyl group is derived from coconut oil or a lauryl group, such as cocoyl amphoteric dipropionate. Such cocoyl amphoteric dipropionate surfactants are the same as amphoteric surfactants of the same type, and may be commercially available under the Miranol series trademark.

[0029] Commercially available useful amphoteric surfactants include: × Cocoyl amphoacetate (sold under the trademarks MIRANOL ULTRA C-32 and MIRAPON FA), × Cocoyl amphopropionate (sold under the trademarks MIRANOL CMSF CONC. and MIRAPON FAS), × Cocoyl amphodiacetate (sold under the trademarks MIRANOL C2M CONC. and MIRAPON FB), × Lauroyl amphoacetate (sold under the trademarks MIRANOL HM CONC. and MIRAPON LA), × Lauroyl amphoteric diacetate (sold under the trademarks MIRANOL H2M CONC. and MIRAPON LB), × Lauroyl amphodipropionate (sold under the trademarks MIRANOL H2M-SF CONC. and MIRAPON LBS), × Lauroyl amphodiacetate obtained from a mixture of lauric acid and myristic acid (sold under the trademark MIRANOL BM CONC.), × Cocoyl amphopropyl sulfonate (sold under the trademark Miranol CS CONC.), ×Hexanoyl amphoteric diacetate (sold under the trademark MIRANOL S2M CONC.), ×Hexanoyl amphoacetate (sold under the trademark MIRANOL SM CONC.), ×Hexanoyl amphodipropionate (sold under the trademark MIRANOL S2M-SF CONC.) and × Stearoyl amphoacetate (sold under the trademark MIRANOL DM). The most preferred other amphoteric surfactant used is cocoyl amphoacetate.

[0030] The other amphoteric surfactants comprise from about 0.1% to about 7% by weight, preferably from about 0.2% to about 4% by weight, and more preferably from 0.3% to 3% by weight, based on the total weight of the hair care composition.

[0031] cationic surfactants Cationic surfactants can provide cations that adhere to hair during washing, straightening the hair, reducing tangles, and improving combability, thereby providing the hair care composition with the functionality of such cations. At the same time, the cations can interact with nicotinamide mononucleotide in an aqueous solution to improve its stability in liquid form. Examples of cationic surfactants include amine salt heterocyclic quaternary ammonium salt-type cationic surfactants. Specific examples of cationic surfactants include at least one of cetyltrimethylammonium chloride, dodecyltrimethylammonium chloride, tricetylmethylammonium chloride, stearyldimethylbenzylammonium chloride, di(partially hydrogenated tallow)dimethylammonium chloride, polyquaternium-10, guar hydroxypropyltrimethylammonium chloride, etc., with polyquaternium-10, guar hydroxypropyltrimethylammonium chloride, or polyethylene glycol being preferred.

[0032] In a specific embodiment of the present invention, the cationic surfactant is present in an amount of 0.01 to 0.09 wt %, preferably 0.02 to 0.08 wt %, more preferably 0.03 to 0.08 wt %, based on the total weight of the hair care composition. In some embodiments of the present invention, the hair care composition further comprises other surfactants such as, for example, nonionic surfactants and other amphoteric surfactants. Nonionic surfactants can function as emulsifiers and stabilizers in the composition. As used herein, "nonionic surfactant" includes mixtures of nonionic surfactants.

[0033] Further examples of useful nonionic surfactants include condensation products of ethylene oxide with a hydrophobic moiety, the hydrophilic-lipophilic balance (HLB) of which is between about 8 and about 16, more preferably between about 10 and about 12.5. These surfactants include condensation products of ethylene oxide with aliphatic primary or secondary alcohols having from about 8 to about 24 carbon atoms in a linear or branched configuration, where the amount of ethylene oxide is from about 2 to about 40 moles, preferably from about 2 to about 9 moles, per mole of alcohol.

[0034] In a preferred embodiment, the aliphatic alcohol contains from about 9 to about 18 carbon atoms and is ethoxylated with from about 3 to about 12 moles of ethylene oxide per mole of aliphatic alcohol. Particularly preferred are primary alcohols containing from about 12 to about 15 carbon atoms and ethoxylated with from about 5 to about 9 moles of ethylene oxide per mole of alcohol. One such material is sold by Shell Chemical Company under the trade name Neodol 25-9. Other commercially available nonionic surfactants include Neodol 25-6.5 and Neodol 25-7, both sold by Shell Chemical Company.

[0035] Other suitable nonionic surfactants include the condensation products of alkylphenols having from about 6 to about 12 carbon atoms with from about 3 to about 30 moles, preferably from about 5 to about 14 moles, of ethylene oxide. Examples of such surfactants are sold by Rhodia, Inc. under the trade names IGEPAL CO 530, IGEPAL CO 630, IGEPAL CO 720, and IGEPAL CO 730. The most preferred nonionic surfactants are ethoxylates of mixed linear alcohols, such as Laureth-7, sold by Rhodia, Inc. under the trade name RHODASURF L-790.

[0036] antioxidants Antioxidants can effectively prevent oxidation, preventing oxidation products such as radicals generated during the oxidation process from subsequently undergoing unpredictable chemical reactions with key components, resulting in deterioration and decomposition of these components over time. Nicotinamide mononucleotide undergoes oxidation in liquid form and decomposes into substances such as nicotinamide. Therefore, the use of antioxidants can effectively protect the stability of nicotinamide mononucleotide in liquid form. Antioxidants can be broadly classified into two types: synthetic antioxidants and natural antioxidants. These two types of antioxidants differ in the chemical structure they use to protect nicotinamide mononucleotide in liquid form. Natural antioxidants are preferably used. Natural antioxidants can be further classified into amino acid-based antioxidants and vitamin-based antioxidants, and are preferably selected from the group consisting of L-cysteine, DL-methionine, vitamin A, vitamin C, vitamin E, vitamin P, and sodium phytate, either singly or in combination. In addition to their essential effects, antioxidants also function as preservatives to a certain extent, synergistically contributing to the overall stability of the hair care composition.

[0037] In a specific embodiment of the present invention, the content of the antioxidant is 0.5 to 3 wt %, preferably 0.9 to 2.5 wt %, more preferably 1.5 to 2 wt %, based on the total weight of the hair care composition.

[0038] Acidity regulator Nicotinamide mononucleotide has improved stability in a liquid state in a weakly acidic environment, so it is necessary to use an acidity regulator in the hair care composition to control the pH. The acidity regulator in the hair care composition may be citric acid, oxalic acid, lactic acid, or the like, either singly or in combination.

[0039] In a specific embodiment of the present invention, Acidity regulator The content of is 1 to 3 wt %, preferably 1.5 to 2.5 wt %, more preferably 2 to 2.5 wt %, based on the total weight of the hair care composition.

[0040] stabilizers Nicotinamide mononucleotide, its salt, or its hydrate is usually very unstable and easily decomposed in a composition, so the hair care composition of the present invention further contains an active additive for stabilizing nicotinamide mononucleotide, its salt, or its hydrate. The stabilizer may be one or more selected from sialic acid, theanine, beta-carotene, and sodium phosphate.

[0041] Other ingredients Other ingredients may be added to the hair care composition to improve its appearance, texture, fragrance, properties, and user experience, as long as the ingredients or combinations thereof do not undergo any chemical reaction with nicotinamide mononucleotide to cause significant degradation of nicotinamide mononucleotide during the shelf life, and at the same time, the ingredients may improve the stability of nicotinamide mononucleotide, its salt, or its hydrate in liquid to a certain extent.

[0042] In terms of appearance and texture, a conditioning agent (e.g., propylene glycol) may be added to the hair care composition to make it transparent, opaque, or colored, and to change its texture from aqueous to various viscosities. Propylene glycol can be used to increase the liquid viscosity of the hair care composition; manufacturers can adjust the viscosity according to the product's positioning and proportionally increase the viscosity by increasing the content of propylene glycol in the hair care composition. In a specific embodiment of the present invention, the content of other ingredients (e.g., propylene glycol) is 0.01 to 1 wt %, preferably 0.1 to 0.8 wt %, and more preferably 0.2 to 0.6 wt %, based on the total weight of the hair care composition.

[0043] A cosolvent may be further added to the hair care composition to adjust the physical texture, particularly the transparency of the liquid feel, of the hair care composition. Sodium xylene sulfonate can improve the transparency of liquid hair care products, such as shampoos, conditioners, and essences. Sodium xylene sulfonate can increase the ability of water molecules in liquids to dissolve ingredients, promoting more complete bonding of each ingredient in the hair care composition. Nicotinamide mononucleotide in the hair care composition has higher solubility in the hair care composition, allowing specific products, such as highly concentrated nicotinamide mononucleotide essences, to be prepared to meet various needs and desires of users. Furthermore, by fusing nicotinamide mononucleotide with other ingredients using sodium xylene sulfonate, some products in the hair care composition can simultaneously perform multiple functions. This means that the use of nicotinamide mononucleotide or a derivative thereof in a hair care composition not only increases the level of coenzyme I in each tissue of the head (e.g., hair, scalp, hair follicles, and cells thereof) to a level that promotes improved hair and head health, but also achieves other purposes (e.g., cleansing, styling, improved odor, etc.), which should also be considered part of the function of the hair care composition. In a specific embodiment of the present invention, the content of sodium xylene sulfonate is 0.01 to 10 wt %, preferably 0.1 to 5 wt %, and more preferably 1 to 3 wt %, based on the total weight of the hair care composition.

[0044] A foaming agent may also be added to a hair care composition to improve the cleansing and repair functions of some monofunctional or multifunctional products in the composition (e.g., shampoo, conditioner, etc.). Foam can create a large contact space, allowing the main ingredient and other ingredients in the hair care composition to diffuse effectively throughout the head, allowing the main ingredient, nicotinamide mononucleotide or a derivative thereof, to be absorbed into the hair, scalp, hair follicles, etc., increasing the coenzyme I level therein, particularly the intracellular mitochondria level, creating favorable conditions for daily metabolism and biocatalysis, and also protecting and repairing against damage caused by environmental pollution and ultraviolet light exposure.

[0045] The foam can extend the time ingredients remain on the scalp, and the tension between the bubbles holds them together, making them more easily retained between the hair and scalp, and its effectiveness is not affected by differences in head shape. Therefore, adding a foaming agent to a hair care composition also plays a certain physical role in increasing the level of coenzyme I. Foaming agents that can be used in hair care compositions include quaternary ammonium polyethylene glycol alkyl amino acid saccharide surfactants, such as betaine, cocamidopropyl betaine, cocoyl monoethanolamine, sodium cocoyl glycinate, sodium lauryl glucose carboxylate, and olive oil fatty acid polyethylene glycol ester mono(carboxymethyl) ether sodium salt. Depending on the function of the product, the foaming agent can be added to the ingredients of the hair care composition. These foaming agents can be used alone in the product ingredients or mixed in various ratios to optimize the foam state of the product and achieve the best usage effect and user experience. As an ingredient of a hair care composition, the foaming agent is preferably betaine, sodium cocoyl glycinate, or sodium lauryl glucose carboxylate, and more preferably sodium cocoyl glycinate. In a specific embodiment of the present invention, the content of sodium cocoyl glycinate is 0.01 to 10 wt %, preferably 0.1 to 5 wt %, and more preferably 0.5 to 1 wt %, based on the total weight of the hair care composition. When mixed in various ratios, a combination of sodium cocoyl glycinate and sodium lauryl glucose carboxylate is most preferred. The ratio of sodium cocoyl glycinate to sodium lauryl glucose carboxylate is 2:1 for fine bubbles and 1:2 for large bubbles, and the bubble size can be proportionally adjusted by the ratio of sodium cocoyl glycinate to sodium lauryl glucose carboxylate in the mixture. In a specific embodiment of the present invention, the content of the entire mixture is 0.01 to 10 wt %, preferably 0.1 to 5 wt %, and more preferably 0.5 to 1 wt %, based on the total weight of the hair care composition.

[0046] Other additives may be added to hair care compositions to improve their appearance, fragrance, feel, and functionality. In terms of appearance, common edible pigments may be added to hair care compositions. For example, dark colors such as yellow, brown, blue, and black can provide significant light-blocking effects and also stabilize the main and other components of the hair care composition. In a specific embodiment of the present invention, the content of the edible pigment is 0.01 to 1 wt %, preferably 0.05 to 0.1 wt %, and more preferably 0.05 to 0.08 wt %, based on the total weight of the hair care composition. Fragrance is important in products containing hair care compositions, such as shampoos, conditioners, and essences, and can enhance user satisfaction with the product while resolving hair odor. Harmless fragrances or refined plant- or mineral-based fragrance oils, more preferably refined plant- or mineral-based fragrance oils, may be added to hair care compositions as the primary fragrance source. Because the ingredients in the hair care composition are unscented, the fragrance provided by the additive is reliably reflected in the hair care composition, but there is no difference between the fragrance of the composition after addition and the fragrance of the additive itself. If a difference in fragrance occurs, it would be considered that an unpredictable chemical reaction has occurred between the additive and the hair care composition, which could affect the chemical structure of nicotinamide mononucleotide in the hair care composition and alter the functionality and stability of the hair care composition. It goes without saying that most refined plant- and mineral-based fragrance oils are inert substances, and their addition to a hair care composition would not cause the above-mentioned special situation. In a specific embodiment of the present invention, the content of the refined plant- and mineral-based fragrance oil is 0.001 to 0.1 wt %, preferably 0.005 to 0.01 wt %, and more preferably 0.08 to 0.01 wt %, based on the total weight of the hair care composition.

[0047] The addition of functional additives to hair care compositions can meet various user needs and increase the coenzyme I levels in hair, scalp, and hair follicle cells, allowing the hair care composition to achieve significant results tailored to the user's expectations within a short period of use. A decrease in coenzyme I levels in scalp epidermal cells can lead to an inability to synthesize ATP to meet daily high-energy needs, and the withering of epidermal cells can increase scalp congestion while also weakening cellular immunity and increasing the risk of infection. Scalp congestion not only affects appearance, but also makes it difficult to remove sweat and scalp sebum from the scalp epidermis, and the increased surface area makes it easier for dust to adhere to the scalp, leading to a simultaneous decline in physiological health and scalp deterioration, which can lead to frequent inflammation of the scalp epidermis, or what is known in the art as severe scalp itching. Scalp itching can lead patients to feel compelled to touch their scalp, and as the itching intensifies, they may scratch their scalp frequently, ultimately seeking to relieve the discomfort as quickly as possible. During this process, patients suffer damage to the scalp epidermis, particularly to hair follicles, resulting in hair loss and the loss of hair growth potential of dead scalp hair follicle cells. This physical damage is partially permanent, and increasing the level of coenzyme I does not promote cellular recovery. The functional additive may be an analgesic or scalp depressant, and this purpose can be achieved by using peppermint oil and zinc pyrithione as functional additives in hair care compositions. Peppermint oil provides users with a refreshing sensation when it comes into contact with the scalp epidermis and relieves the discomfort of scalp itching. This prevents damage to tissues and cells that may occur when users scratch their scalps due to itching. In a specific embodiment of the present invention, the content of peppermint oil is 0.05 to 0.1 wt %, preferably 0.05 to 0.09 wt %, and more preferably 0.06 to 0.08 wt %, based on the total weight of the hair care composition. Zinc pyrithione is an antibacterial agent that can inhibit the growth of gram-negative bacteria, gram-positive bacteria and fungi, and is widely used to remove dandruff with excellent efficacy.In a specific embodiment of the present invention, the content of zinc pyrithione is 0.1 to 1.5 wt %, preferably 0.5 to 1 wt %, more preferably 0.8 to 1 wt %, based on the total weight of the hair care composition.

[0048] In some embodiments of the present invention, the hair care product of the hair care composition is in at least one form selected from shampoo, conditioner, essence, soap, spray, cream, hair wax, gel, paste, or any liquid or solid. Therefore, the hair care product of the present invention can simultaneously have other functions such as cleansing, repair, anti-dandruff, and hairstyle fixation. For example, a shampoo containing nicotinamide mononucleotide not only has a cleansing effect, i.e., the effect of generally removing oil and grease from the hair, but also has foaming properties and is easily absorbed into the hair and scalp.

[0049] In a specific embodiment of the present invention, the shampoo may contain adjuvants such as water, nicotinamide mononucleotide, a salt thereof or a hydrate thereof, a surfactant, an acidity regulator, a fragrance, etc., to maintain its function, properties, and durability. In a more specific embodiment, the water content is 30% by weight to 90% by weight based on the total weight of the shampoo.

[0050] Products containing hair care compositions include not only hair products but also eyebrow care products, eyelash care products, and beard care products. Eyebrow care products include mainly eyebrow care lotions and eyebrow care creams. Eyebrow care lotions and eyebrow care creams contain 0.01 to 3% (w / v) of nicotinamide mononucleotide, its salt, or its hydrate as the main ingredient, to which adjuvants such as sage oil, green tea extract, and white beeswax are added. Eyelash care products are eyelash care lotion kits, in which the eyelash lotion is applied to the eyelashes with an eyelash brush, touching both the upper and lower eyelashes and their hair follicles. The main ingredient in eyelash lotions is also nicotinamide mononucleotide, its salts, or its hydrates. However, because eyelashes grow slower than scalp hair, a higher concentration of the main ingredient is required to stimulate the growth cycle. Therefore, the nicotinamide mononucleotide, its salts, or its hydrates in eyelash lotions is 1-10% (w / v), and the adjuvants are tea tree extract and cysteine. Because eyelash lotions are used near the eyes, they cannot contain eye-sensitive substances, volatile substances, or materials used to treat eye diseases. The purpose of eyelash care lotion kits is to provide users concerned about eyelash health, especially those with slow or short eyelash growth, with options to improve their condition and to support users in both eye health care and personal grooming. Beard care products include beard care lotions, beard care sprays, and beard care creams. Beard care products are made from 0.1-2% nicotinamide mononucleotide, its salt, or its hydrate as the main ingredient, blended with adjuvants such as peppermint extract, burdock powder or its extract, vitamin A, vitamin E, and cysteine, and are formulated into liquid, spray, or paste forms using water, 0.01-0.05% low-concentration ethanol (v / v), or beeswax as a medium, and users can choose according to their preferences. The purpose of beard care lotions, beard care sprays, and beard care creams is to accelerate beard growth and maintain or restore the original color and luster and health of hair follicles, and their principles are consistent with those of the above-mentioned hair care compositions, thereby providing users with a scientifically based health product for personal styling.

[0051] In another aspect, the present invention further provides a method for preparing a hair care composition, the method comprising the steps of: providing nicotinamide mononucleotide, a salt thereof, or a hydrate thereof; providing an aqueous solution of a base component that stabilizes nicotinamide mononucleotide, a salt thereof, or a hydrate thereof; and mixing nicotinamide mononucleotide, a salt thereof, or a hydrate thereof with the aqueous solution to adjust the pH to obtain a hair care composition. The base component that stabilizes nicotinamide mononucleotide, a salt thereof, or a hydrate thereof may include one or more of anionic surfactants, amphoteric surfactants, cationic surfactants, antioxidants, pH buffers, and acidity adjusters.

[0052] Preferably, the method comprises: (1) mixing one or more aliphatic alcohol polyether sulfate anionic surfactants to obtain a first class surfactant; (2) mixing one or more alkyl acyl amino acid based amphoteric surfactants to obtain a second class surfactant; (3) mixing one or more cationic surfactants to obtain a third-class surfactant; (4) mixing a first-class surfactant, a second-class surfactant, and a third-class surfactant to obtain a composite surfactant; (5) adding nicotinamide mononucleotide to the complex surfactant to obtain a mixture; (6) Adding an amino acid-based antioxidant to the mixture and adjusting the pH value of the mixture to 4.85 to 7.0 using an acidity adjuster to obtain a hair care composition.

[0053] In a more specific embodiment, the surfactant may be ammonium laureth sulfate, TEA-cocoyl glutamate, and MIRANOL. The content of ammonium laureth sulfate is 0.2% to 1.9% by weight, based on the total weight of the shampoo, the content of TEA-cocoyl glutamate is 0.1% to 1.5% by weight, and the content of MIRANOL is 0.3% to 3% by weight, based on the total weight of the shampoo. In a more specific embodiment, the acidity adjuster is potassium dihydrogen phosphate, and the pH of the shampoo is 4.5 to 5.5. In a more specific embodiment, the fragrance may be an organic aroma oil, and different scents can be selected according to market needs. The content of the organic aroma oil is 0.1% to 0.5% by weight. In another more specific embodiment, the fragrance may be different organic aroma oils, and different scents can be selected according to market needs. The content of the organic aroma oil is 0.1% to 0.5% by weight. In a more specific embodiment, the shampoo may further contain sunscreens, preservatives, etc., and the content of these ingredients should not exceed 0.05% by weight of the shampoo.

[0054] In a specific embodiment of the present invention, the essence is primarily used to increase the levels of coenzyme I and reduced coenzyme I in hair follicle cells and relieve scalp itching and inflammation, thereby promoting the health of hair follicle stem cells and activating their activity to synthesize ATP and lactic acid. Therefore, the main component of the essence is nicotinamide mononucleotide, its salt, or its hydrate, and its content is 3% to 25% by weight, preferably 5% to 15% by weight, and more preferably 8% to 12% by weight of the total weight of the ingredients. In a more specific embodiment, the essence components include green tea extract, peppermint oil, sage oil, burdock powder, beeswax, and water. The essence may also contain acetylglutathione and glutathione.

[0055] Additionally, a hair care composition is considered within the scope of this application to the extent that it is a topical hair product to which nicotinamide mononucleotide has been added, and which may increase the level of coenzyme I in hair follicles and epidermal cells to prevent, repair, improve, or reverse aging.

[0056] The preparation of the hair care composition of the present invention will be explained below using shampoo and essence as examples. The shampoo is prepared as follows: Ammonium laureth sulfate, TEA-cocoyl glutamate, and MIRANOL are placed in a container as surfactants. The contents of these three surfactants can be different or equal, and can be adjusted to suit various hair types. Equal contents of the three surfactants are suitable for neutral hair types. Taking neutral hair types as an example, the total content of the three surfactants is 0.1% to 10% by weight, preferably 0.5% to 10% by weight, and more preferably 1% to 10% by weight, based on the total weight of the shampoo. After adding the surfactants and vitamins, pure water is added to a total weight of 80% of the total weight, followed by an acidity adjuster. The acidity adjuster may be potassium dihydrogen phosphate or another buffer, and the pH of the solution is preferably 4.8 to 6, more preferably 5 to 5.5. After adjusting the pH to 4.5 to 5.5, nicotinamide mononucleotide, its salt, or its hydrate may be added. The content of nicotinamide mononucleotide, its salt, or its hydrate is 0.01% to 25% by weight, preferably 0.1% to 5% by weight, and more preferably 0.1% to 1% by weight, based on the total weight of the shampoo. Organic aroma oils, light-blocking agents, preservatives, etc. may also be added, provided that the total content of these ingredients does not exceed 5% of the total weight of the shampoo. After uniform stirring, pure water is added until the total volume is reached and the shampoo is ready to use.

[0057] Users are advised to apply the desired amount of shampoo evenly to the scalp, massage the scalp for 2-5 minutes, and then rinse thoroughly. To achieve the desired effect, the shampoo should be used at least once daily and continuously. The essence is prepared as follows. First, burdock powder, green tea extract, and nicotinamide mononucleotide are placed in a container. The burdock powder content is 1% to 5% by weight, preferably 1% to 4% by weight, and more preferably 1% to 2.5% by weight, based on the total weight of the essence. The green tea extract content is 1% to 5% by weight, preferably 2% to 4% by weight, and more preferably 2% to 2.5% by weight, based on the total weight of the essence. The nicotinamide mononucleotide content is 3% to 25% by weight, preferably 5% to 15% by weight, and more preferably 8% to 12% by weight, based on the total weight of the essence. Sialic acid, theanine, glycyrrhizic acid, glutathione, and S-acetyl-L-glutathione are added as adjuvants. Then, pure water is added to the mixture up to 80% of the total weight, and potassium dihydrogen phosphate, an acidity adjuster, is added until the pH reaches 5 to 5.2, followed by uniform stirring. Peppermint oil and sage oil are added to the above solution. The content of peppermint oil is 0.1% to 1.5% by weight, preferably 0.1% to 1.2% by weight, and more preferably 0.1% to 1% by weight, based on the total weight of the essence. The content of sage oil is 0.1% to 1.5% by weight, preferably 0.1% to 1.2% by weight, and more preferably 0.1% to 1% by weight, based on the total weight of the essence. Finally, white beeswax hot-melted at 62 to 65°C is added at 0.1% of the total amount, and pure water is added until the specified volume is reached, followed by stirring until completely dissolved.

[0058] After washing the hair, the user should apply 5-10ml of the essence directly to the scalp and hair roots, gently massage the head with hands to distribute the essence evenly over the scalp, and wait until the essence is fully absorbed. To achieve the desired effect, the essence should be used at least once daily and continuously.

[0059] The present invention will be further illustrated by the following examples, but these examples should not be construed as limiting the scope of protection of the present invention.

[0060] Example In the following examples, unless specific conditions are specified, the experiments were carried out under normal conditions or conditions recommended by the manufacturer. The materials and devices used in the examples are described below. Nicotinamide mononucleotide: obtained from GeneHarbor (Hong Kong) Biotechnologies Ltd. Ammonium laureth sulfate: obtained from Jining Baichuan Chemical Co., Ltd. TEA-cocoyl glutamate: obtained from Beijing Lingbao Technology Co., Ltd. MIRANOL: Obtained from Suzhou Yuantai Run Chemical Co., Ltd. Burdock powder: obtained from Xinghua Hongsheng Food Co., Ltd. Green tea extract: Obtained from Shaanxi Hengling Natural Bioproducts Co., Ltd. Peppermint oil: Obtained from Jiangxi Kippei Natural Vegetable Oil Co., Ltd. Sage oil: Obtained from Shenzhen Ositing Trading Co., Ltd. Potassium dihydrogen phosphate: obtained from Jiangsu Zidong Food Co., Ltd. White beeswax: Obtained from Guangzhou NOF Technology Co., Ltd. S-acetyl-L-glutathione: obtained from GeneHarbor (Hong Kong) Biotechnologies Ltd. Glutathione: obtained from GeneHarbor (Hong Kong) Biotechnologies Ltd. Theanine: Obtained from Merck, USA. Sialic acid: obtained from Merck, USA. Glycyrrhizic acid: Obtained from Merck, USA. Small mixer (LIAEBO LSJB120): Obtained in Changzhou, Jiangsu. pH meter (Raijji PHB-4): Obtained in Shanghai.

[0061] Example 1: Shampoo for neutral hair types containing nicotinamide mononucleotide A 500ml shampoo for neutral hair containing nicotinamide mononucleotide was prepared. 250ml of water, 25g of ammonium laureth sulfate, 25g of TEA-cocoyl glutamate, 1g of miranol, and 50g of vitamin C were added. Pure water was added until the total volume reached 400ml. The mixture was stirred at approximately 100 rpm for 15 minutes using a small mixer until completely homogenous. The pH of the solution was measured using a pH meter (Leizhi PHB-4, Shanghai) and adjusted to pH 5.5 with potassium dihydrogen phosphate. 5g of nicotinamide mononucleotide was then added, and pure water was added until the total volume reached 500ml. The mixture was then stirred until completely homogenous.

[0062] Example 2: Shampoo for dry hair containing nicotinamide mononucleotide A 500ml shampoo for dry hair containing nicotinamide mononucleotide was prepared. It contained 250ml of water, 40g of ammonium laureth sulfate, 10g of TEA-cocoyl glutamate, Vitamin C 50g of nicotinamide mononucleotide was added, and purified water was added until the total volume reached 400ml. The mixture was stirred at approximately 100 rpm for 15 minutes using a small mixer until completely homogenous. The pH of the solution was measured using a pH meter (Leizhi PHB-4, Shanghai) and adjusted to pH 5.5 using potassium dihydrogen phosphate. 5g of nicotinamide mononucleotide was then added, and purified water was added until the total volume reached 500ml. The mixture was stirred until completely homogenous.

[0063] Example 3: Shampoo for oily hair containing nicotinamide mononucleotide A 500ml shampoo containing nicotinamide mononucleotide for oily hair was prepared. It contained 250ml of water, 10g of ammonium laureth sulfate, 40g of TEA-cocoyl glutamate, Vitamin C 50g of nicotinamide mononucleotide was added, and purified water was added until the total volume reached 400ml. The mixture was stirred at approximately 100 rpm for 15 minutes using a small mixer until completely homogenous. The pH of the solution was measured using a pH meter (Leizhi PHB-4, Shanghai) and adjusted to pH 5.5 using potassium dihydrogen phosphate. 5g of nicotinamide mononucleotide was then added, and purified water was added until the total volume reached 500ml. The mixture was stirred until completely homogenous.

[0064] Example 4: Hair essence containing nicotinamide mononucleotide A 50ml volume of hair essence containing nicotinamide mononucleotide was prepared. 25ml of water was added, followed by the addition of 0.5g of burdock powder, 0.4g of sialic acid, 0.2g of glutathione, 0.05g of S-acetyl-L-glutathione, 0.1g of theanine, 0.2g of glycyrrhizic acid, 0.5g of green tea extract, 5g of vitamin C, and 5g of nicotinamide mononucleotide. Next, purified water was added until the total volume reached 35ml, and the mixture was stirred at approximately 100 rpm for 15 minutes using a small mixer until completely homogenous. 0.5g of ammonium laureth sulfate was added, and the pH of the solution was measured using a pH meter and adjusted to 5.5 using potassium dihydrogen phosphate. 500µl of peppermint oil and 500µl of sage oil were then added. While stirring, white beeswax heated to 62-65°C was added in five portions, totaling 0.1g. The mixture was stirred at 250 rpm for 15 minutes using a mixer until completely dissolved.

[0065] Example 5: Anti-itch functional shampoo containing nicotinamide mononucleotide A 500ml anti-itch functional shampoo containing nicotinamide mononucleotide was prepared. It contained 250ml of water, 25g of ammonium laureth sulfate, 25g of TEA-cocoyl glutamate, Vitamin C 50g of nicotinamide mononucleotide and 2.5ml of peppermint oil were added, and the mixture was stirred at about 100 rpm for 15 minutes until completely homogenous. Then, pure water was added until the total volume was 400ml, and the mixture was stirred at about 100 rpm for 15 minutes until completely homogenous. Then, 2.5g of nicotinamide mononucleotide and 2.5ml of peppermint oil were added, and the mixture was stirred at about 100 rpm for 15 minutes until completely homogenous. Finally, pure water was added until the total volume was 500ml, and the mixture was stirred until completely homogenous.

[0066] Example 6: Anti-dandruff functional shampoo containing nicotinamide mononucleotide A 500ml anti-dandruff functional shampoo containing nicotinamide mononucleotide was prepared. It contained 250ml of water, 25g of ammonium laureth sulfate, 25g of TEA-cocoyl glutamate, Vitamin C 50g of nicotinamide mononucleotide and 5g of zinc pyrithione were added, and purified water was added until the total volume was 400ml. The mixture was stirred at about 100 rpm for 15 minutes using a small mixer until it was completely homogenous. The pH of the solution was measured with a pH meter and adjusted to pH 5.5 using potassium dihydrogen phosphate. 2.5g of nicotinamide mononucleotide was then added, and purified water was added until the total volume was 500ml, and the mixture was stirred until it was completely homogenous.

[0067] Example 7: Multifunctional shampoo containing nicotinamide mononucleotide A multifunctional shampoo containing nicotinamide mononucleotide is a product that has both anti-itch and anti-dandruff functions. 500 ml of the multifunctional shampoo containing nicotinamide mononucleotide was prepared. It contained 250 ml of water, 25 g of ammonium laureth sulfate, 25 g of TEA-cocoyl glutamate, and 100 ml of ethanol. Vitamin C 50g and 5g of zinc pyrithione were added, purified water was added until the total volume was 400ml, and the mixture was stirred at about 100 rpm for 15 minutes using a small mixer until completely homogenous. The pH of the solution was measured with a pH meter and adjusted to pH 5.5 using potassium dihydrogen phosphate. 2.5g of nicotinamide mononucleotide and 2.5ml of peppermint oil were then added, and the mixture was stirred at about 100 rpm for 15 minutes until completely homogenous. Purified water was added until the total volume was 500ml, and the mixture was stirred until completely homogenous.

[0068] Example 8: Eyebrow care lotion containing nicotinamide mononucleotide To prepare 50ml of eyebrow care lotion containing nicotinamide mononucleotide, first add 0.5g of nicotinamide mononucleotide, then add 0.5g of green tea extract, and stir the ingredients in 20ml of purified water until completely dissolved. The ingredients were added in the following order and amounts: 1. 0.25g of cysteine ​​hydrochloride, 2. 0.25g of vitamin C, 3. 0.1g of vitamin E, 4. 0.5ml of sage oil, 5. 0.05g of sodium citrate, 6. 0.1g of citric acid, and 7. 0.05g of cocoyl amino acid salt. Stirring was continued until completely dissolved, and then purified water was added to the mixture until the total volume reached 50ml. The pH value of the eyebrow care lotion should be between 4.75 and 5. A 1M sodium citrate solution or a 1M citric acid solution may be used to adjust the pH of the eyebrow care lotion. To use the eyebrow care lotion, apply approximately 1 ml of the liquid to your fingertips in the morning and evening, then lightly apply it to your eyebrows and around your eyes, rubbing the eyebrow care lotion back and forth until it is completely absorbed.

[0069] Example 9: Eyebrow care cream containing nicotinamide mononucleotide To prepare 50 g of eyebrow care cream containing nicotinamide mononucleotide, first add 0.5 g of nicotinamide mononucleotide, then add 0.5 g of green tea extract, and stir the ingredients in 20 ml of purified water until completely dissolved. The ingredients were added in the following order and amounts: 1. 0.25 g of cysteine ​​hydrochloride, 2. 0.25 g of vitamin C, 3. 0.1 g of vitamin E, 4. 0.5 ml of sage oil, 5. 0.05 g of sodium citrate, 6. 0.1 g of citric acid, and 7. 0.05 g of cocoyl amino acid salt. Stirring was continued until completely dissolved, and then purified water was added to the final volume of 45 ml. The pH value was measured using a pH meter. The pH of the solution should be between 4.75 and 5. A 1 M sodium citrate solution or a 1 M citric acid solution may be used to adjust the pH of the solution. Then, while stirring, a total of 2g of white beeswax heated to 62-65°C was added little by little to gradually change the solution from liquid to a solid paste. To use the eyebrow care cream, apply a small amount to your fingertips in the morning and evening, then lightly apply it to your eyebrows and around your eyes, rubbing the cream back and forth until it is completely absorbed.

[0070] Example 10: Eyelash care lotion kit containing nicotinamide mononucleotide To prepare 50ml of eyelash care lotion containing nicotinamide mononucleotide, first add 5g of nicotinamide mononucleotide, 1g of tea tree extract, 1g of green tea extract, 0.5g of L-cysteine ​​hydrochloride, 0.5g of vitamin C, and 0.1g of vitamin D, then add 40ml of purified water and stir to completely dissolve. After measuring the pH with a pH meter, the solution was adjusted to pH 6.75-7 with 0.5M NaOH and filtered through a 0.22uM filter membrane to complete the preparation. It is recommended that users apply approximately 1-2ml of eyelash care lotion to their eyelashes with a brush, tracing it along the eyelid until it is fully absorbed by the eyelashes, morning and night.

[0071] Example 11: Beard care lotion containing nicotinamide mononucleotide To prepare 50ml of beard care lotion, first add 0.5g of nicotinamide mononucleotide, 0.1g of burdock powder, 0.08g of vitamin A, 0.05g of vitamin E, and 0.1g of L-cysteine ​​hydrochloride, then add 40ml of purified water and stir to completely dissolve. Then, measure the pH with a pH meter and adjust the solution's pH to 5.25-5.5 with 1M NaOH. After adjusting, add 0.01g of glyceryl cocoate and 0.05ml of absolute ethanol and stir until completely dissolved. To use, users apply 1-2ml of care lotion to the beard area in the morning and evening, gently massaging until the lotion is fully absorbed into the skin.

[0072] Example 12: Beard care cream containing nicotinamide mononucleotide To prepare 50g of beard care cream, first add 0.5g of nicotinamide mononucleotide, 0.1g of burdock powder, 0.08g of vitamin A, 0.05g of vitamin E, and 0.1g of L-cysteine ​​hydrochloride, then add 40ml of purified water and stir until completely dissolved. Then, measure the pH with a pH meter and adjust the solution to 5.25-5.5 with 1M NaOH. After adjusting, add 0.01g of glyceryl cocoate and stir until completely dissolved. Then, add 1g of white beeswax preheated to 60-65°C in small amounts and continue stirring. Over time, the solution gradually solidifies and becomes a cream, marking the end of the product. Users apply a small amount of the care cream to beard growth areas morning and night, massaging repeatedly until absorbed into the skin.

[0073] Example 13: Shampoo concentrate (without nicotinamide mononucleotide) 500 ml of shampoo stock solution was prepared by adding 250 ml of water, 25 g of ammonium laureth sulfate, 25 g of TEA-cocoyl glutamate, and 50 g of vitamin C, followed by adding purified water until the total volume reached 400 ml. The mixture was stirred at approximately 100 rpm for 15 minutes using a small mixer until completely homogenous. The pH of the solution was measured with a pH meter and adjusted to pH 5.5 using potassium dihydrogen phosphate. Purified water was then added until the total volume reached 500 ml, and the mixture was stirred until completely homogenous.

[0074] Example 14: Hair essence concentrate (without nicotinamide mononucleotide) A 50ml stock solution of hair essence was prepared. 25ml of water was added, followed by the addition of 0.5g of burdock powder, 0.4g of sialic acid, 0.2g of glutathione, 0.05g of S-acetyl-L-glutathione, 0.1g of theanine, 0.2g of glycyrrhizic acid, 0.5g of green tea extract, and 5g of vitamin C, in that order. Next, purified water was added until the total volume reached 35ml, and the mixture was stirred at approximately 100 rpm using a small mixer for 15 minutes until completely homogenous. 0.5g of ammonium laureth sulfate was added, and the pH of the solution was measured using a pH meter and adjusted to 5.5 using potassium dihydrogen phosphate. Next, 500µl of peppermint oil and 500µl of sage oil were added, and while stirring, white beeswax heated to 62-65°C was added in five portions for a total of 0.1g. The mixture was stirred at 250 rpm using a mixer for 15 minutes until completely dissolved.

[0075] Example 15: (Comparative Example, Antioxidant Added) A 500 ml solution of Example 5 was prepared. 250 ml of water and 50 g of vitamin C were added, followed by the addition of purified water until the total volume reached 400 ml. The mixture was stirred at approximately 100 rpm using a small mixer for 15 minutes until completely homogenous. The pH of the solution was measured with a pH meter and adjusted to pH 5.5 using potassium dihydrogen phosphate and dipotassium hydrogen phosphate. 5 g of nicotinamide mononucleotide was then added, followed by the addition of purified water until the total volume reached 500 ml, and the mixture was stirred until completely homogenous.

[0076] Example 16: (Comparative Example, Low Dose Surfactant Added) A 500ml shampoo stock solution was prepared. 250ml of water, 0.25g of ammonium laureth sulfate, 0.25g of TEA-cocoyl glutamate, and 50g of vitamin C were added, followed by adding purified water until the total volume reached 400ml. The mixture was stirred at approximately 100 rpm for 15 minutes using a small mixer until completely homogenous. The pH of the solution was measured with a pH meter and adjusted to 5.5 using potassium dihydrogen phosphate. 5g of nicotinamide mononucleotide was then added, followed by adding purified water until the total volume reached 500ml, followed by stirring until completely homogenous.

[0077] Study 1 - Comparison of the effects of shampoos containing nicotinamide mononucleotide and control shampoos Sixteen male and 16 female subjects aged 50 years or older were recruited. Before the study, researchers assessed and scored the participants' hair color, growth rate, dandruff status, and hair distribution (Tables 1-4, Methods Table 11), and confirmed that they had not dyed, permed, or used negative ion hair treatments in the past year. Participants also agreed to refrain from any of the above-mentioned activities within 90 days of the study, and that if they needed to cut or care for their hair, they would need to contact the project staff, as appropriate arrangements, including reevaluation, would be required.

[0078] The study was conducted twice by gender, and the researchers randomly distributed shampoos prepared by the researchers according to Example 1 or Example 13 above in a double-blind manner. Participants were required to wash their hair with the designated shampoo at least once every 24 hours. Each wash involved using 2-5 ml of shampoo, leaving both the shampoo and lather on the scalp for at least two minutes, and then rinsing thoroughly with warm water. Subjects were evaluated every 30 days, with the total study period lasting 90 days. The test results are shown in Tables 5-6 below.

[0079] [Table 1]

[0080] [Table 2]

[0081] [Table 3]

[0082] [Table 4]

[0083] [Table 5]

[0084] [Table 6]

[0085] Test 2 - Comparison of the effects of a hair essence containing nicotinamide mononucleotide and a control hair essence A hair essence containing nicotinamide mononucleotide was prepared according to Example 4, and a hair essence concentrate was prepared according to Example 14. Eight male and eight female subjects were recruited as participants and included in the study. Participants were required to be healthy and aged 50 or older. Prior to the study, researchers assessed and scored the participants' hair color, growth rate, dandruff status, and hair distribution (see Tables 1-4). They also confirmed that participants had not undergone any hair dyeing, perms, or negative ion hair treatments, or had undergone any procedures (including surgery or other medical treatments) that could affect hair growth within the past year. Participants also agreed to refrain from any of the above-mentioned procedures within 90 days of the study. If they needed to undergo haircuts or other treatments, they would need to contact the project staff for appropriate arrangements, including reevaluation.

[0086] The study was conducted in two separate periods, separated by gender, and was a double-blind study. Researchers randomly distributed either a nicotinamide mononucleotide-containing hair essence prepared according to Example 4 or a hair essence concentrate prepared according to Example 14. The researchers instructed participants to use the product twice daily. After washing their hair in the morning and evening, they dried it with a dry towel (without using a hair dryer), then immediately applied 5-10 ml of the hair essence to their scalp and massaged it evenly to distribute it evenly. This process continued for at least 5 minutes until the hair essence was fully absorbed into the scalp. Subjects were evaluated before and every 30 days after the start of the study, for a total study period of 90 days. The test results are shown in Tables 7-9 and Figures 3-4 below.

[0087] [Table 7]

[0088] [Table 8]

[0089] Furthermore, photographs of the female subject's hair growth were taken, and the hair growth density was calculated. See Figures 3(a)-(d), Figure 4, and Table 9 for the results. Figures 3(a)-(d) are photographs showing the hair growth status of a female subject who used the hair essence containing nicotinamide mononucleotide of Example 4 on various days. Specifically, these photographs demonstrate the effects of the female subject's use of the hair essence within 90 days. The red line indicates the parting of the hair, and the average hairline distance is the distance measured on both sides of the hair by placing six points on the parting line using Adobe Photoshop CS software. A larger distance indicates sparse hair distribution, while a smaller distance indicates dense hair distribution. Table 9 shows the average hairline spacing for each day for female subjects who used the hair essence containing nicotinamide mononucleotide of Example 4. Figure 4 shows the change over the number of days in the average hair spacing for female subjects who used the hair essence containing nicotinamide mononucleotide of Example 4.

[0090] [Table 9]

[0091] Test 3: Nicotinamide mononucleotide stability test A test to compare the stability of nicotinamide mononucleotide was conducted using aqueous nicotinamide mononucleotide solutions (2.5 g of nicotinamide mononucleotide dissolved in 500 ml of purified water) from Examples 1, 15, and 16. Specifically, samples were placed under normal conditions (temperature 22°C and humidity 65%) and accelerated conditions (temperature 37°C and humidity 85%). A 30-day study was conducted daily using high-performance liquid chromatography to observe the stability of nicotinamide mononucleotide in each group. See Figures 1 and 2 for stability results, and Table 10 for test methods and equipment.

[0092] [Table 10]

[0093] Control 1: Oral nicotinamide mononucleotide supplement only Subjects took two 300 mg oral nicotinamide mononucleotide supplements (obtained from GeneHarbor (Hong Kong) Biotechnologies Ltd.: NMN9000) daily after breakfast. After three months, they completed a questionnaire to assess their physical health, hair growth rate, hair color, and the amount of newly grown hair. Subject feedback indicated significant improvements in mental state, physical strength, sleep quality, and skin. Specifically, subjects reported slightly longer deep sleep duration, improved athletic performance, faster recovery, and no minor physical illnesses or symptoms over the past three months. They also reported significant reductions in dry mouth, hot flashes, intermittent mental discomfort, and chills and fevers commonly seen in the elderly. Personally, they reported improved hearing and vision, becoming more sensitive. During the study, researchers discovered that a handful of black hair had grown near the back of the subject's head, a stark contrast to the gray hair in the same location three months earlier. Comparing hair growth rate, hair color change, and new hair growth, there was a slight improvement compared to three months prior, but the effect was slower than when using a shampoo for neutral hair containing nicotinamide mononucleotide and a hair essence containing nicotinamide mononucleotide.The subjects reported that the most noticeable effect after taking the product was a reduction in hair loss, and that scalp itching and dandruff were also significantly reduced.

[0094] Control Example 2: Taking nicotinamide mononucleotide supplements in combination with hair essence containing nicotinamide mononucleotide Each day after breakfast, subjects took two 300 mg oral nicotinamide mononucleotide supplements (obtained from GeneHarbor (Hong Kong) Biotechnologies Ltd.: product name - NMN9000) and applied 5 ml of hair essence daily after washing their hair. After three months, subjects completed a questionnaire to assess their physical health, hair growth rate, hair color change, and the amount of newly grown hair. Subjects reported significant improvements in their physical condition, including increased stamina. Most notably, they no longer felt short of breath or fatigue when climbing mountains or stairs, and their skin felt firmer. Although they slept less than before, they still felt energized and did not have trouble waking up or falling back asleep. Insomnia and frequent urination were slightly reduced, resulting in a significant improvement in their quality of life. When comparing hair growth rate, changes in hair color, and newly grown hair, the difference was significant compared to three months prior, and in particular, black hairs had noticeably grown in areas where there had been only a single clump of white hair before use, accounting for around 20% of the hair. The researchers took 10 each of the white and black hairs and studied them in detail under an electron microscope, concluding the following:

[0095] [Table 11]

[0096] Compared to subjects who only took oral nicotinamide mononucleotide supplements, the subjects' hair growth was more pronounced, and the hairs that grew were more likely to be dark. It should be noted that the scope of application of the present invention is not limited to the above embodiments, and those skilled in the art may make modifications or substitutions based on the above description, all of which are within the scope of protection of the appended claims of the present invention.

[0097] [Table 12]

Claims

1. A hair care composition comprising nicotinamide mononucleotide, a salt thereof, or a hydrate thereof, and a base component that stabilizes nicotinamide mononucleotide, a salt thereof, or a hydrate thereof, the base component that stabilizes nicotinamide mononucleotide, its salt, or its hydrate comprises an anionic surfactant, an amphoteric surfactant, an antioxidant, and an acidity regulator; the anionic surfactant comprises a fatty alcohol polyether sulfate; and The amphoteric surfactant includes an alkyl amino acid type amphoteric surfactant. A hair care composition comprising:

2. The nicotinamide mononucleotide, its salt, or its hydrate is in the form of a solid, liquid, or mist.

2. The hair care composition of claim 1.

3. The hair care composition may be in the form of a shampoo, conditioner, essence, soap, spray, cream, hair wax, gel, or paste.

2. The hair care composition of claim 1.

4. The product containing the hair care composition is any one of an eyebrow care product, an eyelash care product, and a beard care product.

2. The hair care composition of claim 1.

5. The base component contains one or more selected from sialic acid, theanine, β-carotene, and sodium phosphate, and prevents nicotinamide mononucleotide from being decomposed when in liquid or mist form.

2. The hair care composition of claim 1.

6. The nicotinamide mononucleotide, its salt, or its hydrate accounts for 0.01% by weight to 25% by weight of the total weight of the hair care composition, calculated as nicotinamide mononucleotide.

2. The hair care composition of claim 1.

7. Further containing water, The content of water is 50 to 95% by weight based on the total weight of the hair care composition.

2. The hair care composition of claim 1.

8. Further comprising a cationic surfactant and a pH buffer, relative to the total weight of the hair care composition, The content of the anionic surfactant is 15 to 65% by weight, The content of the amphoteric surfactant is 1 to 15% by weight, The content of the cationic surfactant is 0.01 to 0.09% by weight, The content of the antioxidant is 0.5 to 3 wt %; The content of the acidity regulator is 0.05 to 1% by weight, The content of the pH buffer is 1 to 3% by weight.

2. The hair care composition of claim 1.

9. The anionic surfactant further includes at least one selected from sodium lauryl sulfate, ammonium lauryl sulfate, sodium methyl cocoyl taurate, laureth-5 carboxylic acid, sodium lauryl glucose carboxylate, and mixtures thereof.

9. The hair care composition of claim 1 or 8.

10. The amphoteric surfactant further comprises at least one of cocamidopropyl betaine, cocoyl methyl dextran, or a mixture thereof.

9. The hair care composition of claim 1 or 8.

11. The cationic surfactant is at least one selected from polyquaternium-10, guar hydroxypropyltrimethylammonium chloride, or a mixture thereof; the antioxidant is at least one selected from L-cysteine, DL-methionine, vitamin A, vitamin C, vitamin E, sodium phytate, or a mixture thereof; The acidity regulator is at least one selected from citric acid, oxalic acid, lactic acid, hydrochloric acid, and mixtures thereof.

9. The hair care composition of claim 8.

12. The eyebrow care products are eyebrow care lotions, eyebrow care sprays, and eyebrow care creams, The eyelash care product is an eyelash care lotion kit, The beard care products are beard care lotion, beard care spray and beard care cream.

5. The hair care composition of claim 4.

13. Further containing functional additives to improve the appearance, texture, fragrance and user experience of the hair care composition, The functional additive is selected from a sensitizer, a foam booster, a fragrance, a functional adjuvant, or any combination thereof.

3. The hair care composition according to claim 1 or 2.

14. providing nicotinamide mononucleotide, a salt thereof, or a hydrate thereof; providing an aqueous solution of a base component that stabilizes nicotinamide mononucleotide, its salt, or its hydrate in liquid or gaseous form; and mixing nicotinamide mononucleotide, a salt thereof, or a hydrate thereof with the aqueous solution and adjusting the pH value to obtain a hair care composition; the base component that stabilizes nicotinamide mononucleotide, its salt, or its hydrate comprises an anionic surfactant, an amphoteric surfactant, an antioxidant, and an acidity regulator; the anionic surfactant comprises a fatty alcohol polyether sulfate; and The amphoteric surfactant includes an alkyl amino acid type amphoteric surfactant.

1. A method for preparing a hair care composition comprising:

15. The base component that stabilizes nicotinamide mononucleotide, its salt, or its hydrate in a liquid or gaseous state includes one or more of a cationic surfactant, a nonionic surfactant, and a pH buffer.

15. A method for preparing a hair care composition according to claim 14.

16. (1) mixing one or more anionic surfactants to obtain a first class surfactant; (2) mixing one or more amphoteric surfactants to obtain a second class surfactant; (3) mixing one or more cationic surfactants to obtain a third-class surfactant; (4) mixing a first-class surfactant, a second-class surfactant, and a third-class surfactant to obtain a composite surfactant; (5) adding nicotinamide mononucleotide to a complex surfactant to obtain a mixture; (6) adding a pH buffer to the mixture and adjusting the pH value of the mixture to 4.85 to 7.0 using an acidity adjuster to obtain a hair care composition; (7) adding an antioxidant to the mixture to obtain a hair care composition.

16. A method for preparing a hair care composition according to claim 15.

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