Use of indole in controlling tea plant pests in the field and method of use

Abstract

To provide a method for controlling tea tree insect pests in a field, which is safe and has an excellent controlling effect on the tea tree insect pests by both of direct action and indirect action of attracting natural enemies.SOLUTION: Indole is used in the control of tea tree pests in the field, preferably, indole solid is dissolved in 10 parts of absolute ethanol and formulated into an indole mother liquid, and after the dissolution is completed, it is diluted with distilled water to an indole hydraulic liquid of 100 mg / L, and within the normal growing cycle of tea trees, tea trees are treated once every 7 days, for a total of 3 passes of treatment, by application of the indole hydraulic fluid by foliar spraying.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention relates to the technical field of plant pest control, and more particularly to the use and method of indole in controlling tea plant pests in the field. [Background technology]

[0002] Tea plants are one of China's most distinctive and economically valuable crops, and the tea industry is a key agricultural industry that enriches the people of China's tea-growing regions and increases their incomes, as well as an important carrier of rural development. However, tea plants are subject to a complex array of pests, causing widespread damage and severe damage, severely impacting China's tea production and resulting in enormous economic losses. How to effectively control tea plant pests has become an important issue that must be resolved as soon as possible in China's agricultural production.

[0003] Indole is a heteroaromatic organic compound that is widely distributed in the natural environment, has volatility, and is released in large amounts when plants are subjected to abiotic or biotic stress, especially when they are damaged by pests.

[0004] Research has shown that, on the one hand, indole can help plants defend themselves against pests by acting as a chemical signaling substance, activating the plant's immune resistance or attracting the pest's natural enemies; on the other hand, it can regulate the physiological responses and resistance of plants by synthesizing pest resistance substances as a precursor of plant secondary metabolites or by being involved in the synthesis of the plant hormone auxin.

[0005] Exposure to pests such as the white geometrid and the tea green leafhopper also induces the synthesis and release of large amounts of volatile indoles.

[0006] Indoor controlled experiments have shown that indole induces early defense signals in tea plants, activates the synthesis of defense hormones, promotes the accumulation of insect-resistant secondary metabolites, and enhances the resistance of tea plants to the white moth. These findings suggest that indole plays an important role in the tea plant's response to insect stress. Indole is also a key aroma compound in tea leaves; for example, indole is one of the most important aroma-imparting components in floral oolong tea.

[0007] Currently, the main method of controlling tea plant pests is still the application of chemical pesticides, and the series of problems caused by pesticide residues are seriously threatening the quality and safety of tea leaves and the ecological safety of tea plantations.

[0008] Tea, as a healthy beverage recognized worldwide, is drawing increasing attention and demand for its quality and safety, and there is also a growing need for safe tea and organic tea. Therefore, developing green pest control techniques in tea plantations, controlling tea plant pests with more environmentally friendly, scientific, and efficient means, and reducing and even replacing the use of chemical pesticides are important missions that must be accomplished urgently in the future, and are also effective means of ensuring the green and healthy development of China's tea industry.

[0009] Indole is a plant-derived compound, and its use to control pests has the advantages of being green, harmless, and non-polluting to the environment. It has been reported that indole can enhance plant pest resistance and has potential for use in the field of plant pest control, but there have been no reports of its use in controlling tea plant pests in fields.

[0010] Therefore, the present invention increases the resistance of tea plants to pests by spraying indole on the leaves, thereby reducing losses in the tea industry caused by pests, which is of great significance for developing green pest control technology in tea plantations and stabilizing safe production in the tea industry. Summary of the Invention [Problem to be solved by the invention]

[0011] The present invention addresses the problems existing in the prior art by preventing tea plant pests in fields. These include the tea green leafhopper, the citrus spiny whitefly, the whitefly, the pale-striped geometrid and the phylloxera aphid. The present invention aims to design and provide a method for using indole in the control of pests. [Means for solving the problem]

[0012] The object of the present invention is achieved by the following technical solutions:

[0013] One aspect of the present invention is to prevent tea plant pests in fields. These include the tea green leafhopper, the citrus spiny whitefly, the whitefly, the pale-striped geometrid and the phylloxera aphid. The present invention provides the use of indole in the control of

[0014] Another aspect of the present invention provides a method for using indole to control tea plant pests in fields, including the tea green leafhopper, the citrus spiny whitefly, the whitefly, the pale yellow geometrid and the polytrichum aphid, specifically by spraying indole onto the surface of tea plant leaves during the normal growth cycle of the tea plant. Furthermore, the indole solid was first dissolved in 10 parts of absolute ethanol to obtain the indole mother liquor. Prepare After dissolution is complete, add distilled water to make a 100 mg / L indole working solution. Prepare and prepare for use. Furthermore, during the normal growth cycle of tea plants, the tea plants are treated with the indole working solution by foliar spraying once every seven days for a total of three treatments. [Effects of the Invention]

[0015] Compared with the prior art, the present invention has the following advantages: (1) The method of using and preparing the indole described in the present invention is simple, and it can be sprayed directly onto plant leaves in the field. (2) In the application process, indole is green, safe, environmentally friendly, economical, and has no adverse effect on plant growth after treatment. (3) Extensive field tests have shown that indole treatment not only significantly reduces the population of pests such as the tea green leafhopper, citrus spine whitefly, and whitefly on tea plants, but also significantly increases the population of natural enemies such as spiders and parasitic wasps on tea plants. This shows that indole has excellent control effects against many tea plant pests, both through direct action and indirect action by attracting natural enemies. [Brief explanation of the drawings]

[0016] [Figure 1] FIG. 1 is a graph showing the effect on the populations of pests such as the tea green leafhopper, the citrus spiny whitefly, and the whitefly in tea fields after the tea plants of the present invention were subjected to a foliar spray treatment of indole. [Figure 2] FIG. 1 shows the effect on the population of natural enemies such as spiders and parasitic wasps in tea fields after tea plants of the present invention have been subjected to indole foliar spray treatment. DETAILED DESCRIPTION OF THE INVENTION

[0017] In order to better understand the present invention, the following will be described: The tea green leafhopper, the citrus spiny whitefly, the whitefly, the pale-striped geometrid and the phylloxera aphid are The use and method of indole in prevention will be further described below in conjunction with specific examples and drawings in the specification. [Example]

[0018] Field trials of indole to control tea plant pests

[0019] 1) Experimental materials: Tested tea plant variety: Longjing 43 (Camellia sinensis). Pests surveyed in the fields: Tea plant pests include the tea green leafhopper (Empoasca onukii), citrus spiny whitefly (Aleurocanthus spiniferus), whitefly (Trialeurodes vaporariorum), tea tree thrips (Scirtothrips dorsalis), pale-striped geometries (Ectropis obliqua), and phylloxera aphid (Toxoptera aurantii). Indole (CAS: 120-72-9): purchased from Sigma-Aldrich (Shanghai) Trading Co., Ltd.

[0020] 2) Experimental method:

[0021] Preparation of indole working solution: Weigh an appropriate amount of indole solid on a balance and place it in a centrifuge tube, then add 10 parts of absolute ethanol to make indole mother solution Prepare After the indole solid is completely dissolved, add an appropriate amount of distilled water to make a 100 mg / L indole working solution. Prepare Equal volumes of absolute ethanol and distilled water served as control working fluids.

[0022] Tea plant treatment: Indole treatment group and control group were set up, each group was divided into four cells, each with an area of ​​0.09 hm 2 The cells were 30m x 30m in size, with a 5m buffer zone between them. For the indole-treated group, the indole working solution was sprayed onto the surface of the tea plant leaves. The amount sprayed varied depending on the size of the tea plant and the number of leaves, but it was preferable to have the indole working solution soak into at least three-quarters of the leaves of the entire tea plant. Treatments were carried out once every seven days, for a total of three treatments. For the control group, the same volume of the control working solution was sprayed.

[0023] Population survey of tea plant pests and natural enemies: After the tea plant treatments, the populations of pests and natural enemies on the tea plants were surveyed every seven days, a total of three times. A zigzag sampling method was used, with five survey sampling points set up per cell. The pot-beating method was used to knock arthropods on each tea plant into white porcelain pots, and the population numbers of each type of arthropod were recorded statistically. The main targets of the survey were tea plant pests such as the tea green leafhopper, citrus spiny whitefly, whitefly, tea tree thrips, white geometries, and phylloxera aphids, while the natural enemies of tea plant pests were spiders and parasitic wasps.

[0024] 3) Experimental results As can be seen from Figure 1, by examining the arthropod community on indole-treated and untreated tea plants, it was found that indole treatment reduced the number of tea plant pests. These include the tea green leafhopper, the citrus spiny whitefly, the whitefly, the pale-striped geometrid and the phylloxera aphid. Compared to the control, indole treatment reduced the tea green leafhopper population by 33.33% to 60%, the citrus spiny whitefly population by 57.14% to 92.31%, and the whitefly population by 40% on tea plants, with little effect on the tea tree thrips population. Inspection of the tea fields showed no evidence of other pests, such as the white-striped geometrid and the phylloxera aphid. As can be seen from Figure 2, indole treatment can also significantly increase the population of natural enemies of tea plant pests. Compared to the control, the number of spiders on tea plants after indole treatment increased by 64.29% to 140%, and the population of parasitic wasps increased by 350%, indicating that indole can protect tea plant pests by attracting their natural enemies.

[0025] From the above results, it is believed that indole has the potential to control tea plant pests in the field both by direct action and by indirect action by attracting natural enemies. These include the tea green leafhopper, the citrus spiny whitefly, the whitefly, the pale-striped geometrid and the phylloxera aphid. It was shown that the compound can effectively control the population of tea plants and has excellent control effects against many tea plant pests in tea plantations, and has the potential for wide-ranging applications.

[0026] Finally, it should be noted that the above embodiments are only used to describe the technical solutions of the present invention, and are not intended to limit the same. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art can still modify the technical solutions described in the above embodiments or equally replace some or all of the technical features thereof, and such modification or replacement will not cause the essence of the corresponding technical solutions to depart from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. The indole solid was dissolved in 10 parts of absolute ethanol to prepare an indole mother solution. After the dissolution was complete, a 100 mg / L indole working solution was prepared with distilled water. A method for using indole to control tea plant pests in fields, including the tea green leafhopper, citrus spiny whitefly, whitefly, pale-striped geometrid and phylloxera aphid, which comprises spraying the indole working solution onto the surface of tea plant leaves during the normal growth cycle of the tea plant.

2. 2. The method according to claim 1, characterized in that the tea plant is treated with the indole working solution by foliar spraying once every seven days, for a total of three treatments during the normal growth cycle of the tea plant.

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