Fat separation and purification module and fat separation and purification device

Abstract

To provide a fat separation refining module and a fat separation refining apparatus which can reduce damage to a fat tissue while removing an unnecessary liquid component included in the fat tissue.SOLUTION: A fat separation refining module 1 includes: a cylindrical member 10 having a first cylindrical part 11, a second cylindrical part 12 having an inner diameter smaller than the inner diameter of the first cylindrical part 11 and having an introduction port 121, and a third cylindrical part 13 having a discharge port 131; a filter member 21 which is movable in a cylindrical shaft J1 inside the cylindrical member 10, brings its peripheral part into contact with the inner wall of the second cylindrical part 12 in a state of being arranged inside the second cylindrical part 12, and forms a gap between the peripheral part and the inner wall of the first cylindrical part 11 in a state of being arranged inside the first cylindrical part 11; and an operation rod 22 which is long, where the filter member 21 is fixed to the end in a -Z direction.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention relates to a fat separation and purification module and a fat separation and purification device. [Background technology]

[0002] Conventionally, adipose tissue has been collected in a bottle connected to a cannula, and then the adipose tissue has been collected from the bottle and directly reinjected into the patient without preparation. Alternatively, the adipose tissue has been manually collected directly with a syringe, then centrifuged, and reinjected into the patient. For example, a device for collecting adipose tissue has been proposed, which includes a jar for storing the collected adipose tissue inside, a lid for sealing the opening of the jar, a first pipe having one end attached to a suction cannula and the other end fixed to the lid, and a second pipe having one end fixed to the lid and the other end connected to a suction pump (see, for example, Patent Document 1). In this device, the inside of the first pipe is connected to the inside of the jar, and the inside of the second pipe is connected to the inside of the jar. The adipose tissue collected in the jar is then collected with a syringe and used. [Prior art documents] [Patent documents]

[0003] [Patent Document 1] Special Publication No. 2022-508908 Summary of the Invention [Problem to be solved by the invention]

[0004] However, while the device described in Patent Document 1 can collect adipose tissue while maintaining a sealed state inside the jar, it is difficult to sufficiently remove unnecessary liquid components contained in the adipose tissue, i.e., water, blood components, oily components, impurities, etc., without causing severe mechanical damage to the adipose tissue. For this reason, for example, in autologous adipose tissue transplantation surgery, there is a risk that the survival rate of the transplanted adipose tissue will be significantly reduced.

[0005] The present invention has been made in consideration of the above-mentioned reasons, and aims to provide a fat separation and purification module and a fat separation and purification device that can remove unnecessary liquid components contained in adipose tissue while reducing damage to the adipose tissue. [Means for solving the problem]

[0006] In order to achieve the above object, the fat separation and purification module according to the present invention comprises: A fat separation and purification module for separating and purifying liquid components from adipose tissue, a cylindrical member having: a first cylindrical section having a bottom and a through hole formed in the bottom wall; a second cylindrical section having a cylindrical shape and a cross-sectional area perpendicular to the cylindrical axis direction that is smaller than the cross-sectional area perpendicular to the cylindrical axis direction of the first cylindrical section, the second cylindrical section being continuous with one end of the first cylindrical section on the opposite side to the bottom wall side in the cylindrical axis direction, and having an inlet for introducing a cleaning solution for purifying adipose tissue inside; and a third cylindrical section being continuous with the other end of the second cylindrical section on the opposite side to the first cylindrical section side in the cylindrical axis direction, and having an outlet for discharging liquid present inside to the outside; a filter member that is movable in the cylindrical member in the cylindrical axis direction, has a size and shape such that when disposed inside the second cylindrical portion, its peripheral portion abuts against the inner wall of the second cylindrical portion, and when disposed inside the first cylindrical portion, a gap is formed between the peripheral portion and the inner wall of the first cylindrical portion, and is disposed inside the second cylindrical portion in an orientation that divides the interior of the cylindrical member into two regions adjacent in the cylindrical axis direction; The filter member is fixed to one end of a long operating rod in the longitudinal direction, and the other end is inserted into the through hole and is positioned outside the cylindrical member. [Effects of the Invention]

[0007] According to the present invention, when the filter member is placed inside the second cylindrical portion using the operating rod and adipose tissue and cleaning solution are introduced into the first cylindrical portion side of the filter member inside the cylindrical member, liquid components contained in the adipose tissue and cleaning solution pass through the filter member inside the cylindrical member, move toward the third cylindrical portion, and are discharged to the outside from the third cylindrical portion. The adipose tissue from which the liquid components have been removed remains in the area of ​​the filter member on the first cylindrical portion side inside the cylindrical member. Then, by moving the filter member toward the inside of the first cylindrical portion using the operating rod and rotating it around the cylindrical axis, the adipose tissue from which the liquid components present on the first cylindrical portion side of the filter member have been removed moves toward the third cylindrical portion side of the filter member through the gap between the circumferential portion of the filter member and the inner wall of the first cylindrical portion. The adipose tissue that has moved toward the third cylindrical portion side is transferred from the third cylindrical portion to a syringe or the like. This allows the adipose tissue from which the liquid components have been removed to be placed in the third tubular section while reducing the load on the adipose tissue, thereby reducing damage to the adipose tissue while removing unnecessary liquid components contained in the adipose tissue. [Brief explanation of the drawings]

[0008] [Figure 1] FIG. 1 is a schematic perspective view of a fat separation and purification module according to a first embodiment of the present invention. [Figure 2] 1 shows a schematic diagram of a fat separation and purification module according to embodiment 1, where (A) shows how adipose tissue is introduced into the inside of a tubular member, and (B) shows how an inert gas is introduced into the inside of a tubular member. [Figure 3] 2 is a cross-sectional view of a tubular member of the fat separating and refining apparatus according to the first embodiment. FIG. [Figure 4]1 shows a schematic diagram of a fat separation and purification module according to embodiment 1, in which (A) shows how adipose tissue is washed by stirring a mixture of adipose tissue and saline, and (B) shows how the filter member is moved to the bottom wall side of the first cylindrical part of the cylindrical member. [Figure 5] 1 shows a schematic diagram of a fat separation and purification module according to embodiment 1, in which (A) shows how the adipose tissue is moved toward the third cylindrical section by rotating the filter member, and (B) shows how the adipose tissue is guided by the blades to the outer periphery of the filter member when the filter member is rotated. [Figure 6] FIG. 10 is a schematic diagram showing how the fat tissue accumulated inside the third cylindrical part is collected by a syringe in the fat separation and purification module according to the first embodiment. [Figure 7] FIG. 10 is a schematic side view showing a part of a fat separating and refining device according to a second embodiment of the present invention. [Figure 8] FIG. 10 is a schematic side view showing a part of a fat separating and refining device according to a modified example. [Figure 9] FIG. 10 is a schematic side view showing a part of a fat separating and refining device according to a modified example. [Figure 10] 10 is a flowchart showing an example of the flow of a fat separating and refining process executed by a fat separating and refining device according to a modified embodiment. DETAILED DESCRIPTION OF THE INVENTION

[0009] (Embodiment 1) The fat separation and purification module according to this embodiment will be described below with reference to the drawings. The fat separation and purification module according to this embodiment is for separating and purifying liquid components from adipose tissue. This fat separation and purification module includes a first cylindrical part that is cylindrical with a bottom and has a through-hole formed in the bottom wall, a second cylindrical part that is cylindrical and has a smaller cross-sectional area perpendicular to the cylindrical axis direction than the cross-sectional area perpendicular to the cylindrical axis direction of the first cylindrical part, and that is continuous with one end of the first cylindrical part opposite to the end on the bottom wall side of the first cylindrical part in the cylindrical axis direction, and has an inlet for introducing a cleaning solution for purifying adipose tissue inside, and a third cylindrical part that is cylindrical and is continuous with the other end of the second cylindrical part opposite to the end on the first cylindrical part side in the cylindrical axis direction, and has an outlet for discharging liquid present inside to the outside. a filter member that is movable in the axial direction inside the cylindrical member, and that has a size and shape such that when placed inside the second cylindrical portion, the peripheral portion abuts the inner wall of the second cylindrical portion, and when placed inside the first cylindrical portion, a gap is formed between the peripheral portion and the inner wall of the first cylindrical portion, and when placed inside the second cylindrical portion, the filter member is arranged in an orientation that divides the interior of the cylindrical member into two adjacent regions in the axial direction; and a long operating rod that has the filter member fixed to one end in the longitudinal direction and has the other end placed outside the cylindrical member when inserted into the through hole.

[0010] The fat separation and purification module according to this embodiment is for separating and purifying liquid components from adipose tissue, and as shown in FIG. 1 , includes a cylindrical tubular member 10 with a bottom, a filter member 21, a control rod 22, and a gas supply pipe 31. The tubular member 10 is formed, for example, from resin and includes a first tubular portion 11, a second tubular portion 12, a third tubular portion 13, and connecting portions 14 and 15. The first tubular portion 11 has a cylindrical shape with a bottom, and a through-hole 11b that is circular in plan view formed in the bottom wall 11a. The first tubular portion 11 also has an inlet 111 provided in the bottom wall 11a for introducing adipose tissue into the inside of the tubular member 10, and an outlet 112 provided in the bottom wall 11a for discharging gas present inside the tubular member 10. Furthermore, the inlet 111 and the outlet 112 are provided with an inlet opening / closing valve 113 and an outlet opening / closing valve 114, respectively. In addition, a blade 41 is provided on the bottom wall 11a inside the first tubular portion 11, which guides fatty tissue adhering to the +Z direction side of the filter member 21 into the gap between the periphery of the filter member 21 and the inner wall of the tubular member 10 when the filter member 21 is rotated while in contact with the filter member 21 inside the first tubular portion 11.

[0011] The second cylindrical portion 12 is cylindrical, and the area of ​​a cross section perpendicular to the cylindrical axis J1 is smaller than the area of ​​a cross section perpendicular to the cylindrical axis direction of the first cylindrical portion 11. In other words, the inner diameter is shorter than the inner diameter of the first cylindrical portion 11. The second cylindrical portion 12 is continuous with the other end of the first cylindrical portion 11 opposite the end of the bottom wall 11a of the first cylindrical portion 11 in the cylindrical axis J1 direction, i.e., the end on the -Z direction side, via a connecting portion 14. The connecting portion 14 is cylindrical, continuous with the first cylindrical portion 11 at the end on the +Z direction side and with the second cylindrical portion 12 at the end on the -Z direction side, and has a shape that expands in diameter toward the +Z direction. The second cylindrical portion 12 further has an inlet 121 for introducing a cleaning solution for purifying adipose tissue. The inlet 121 is provided with an inlet opening / closing valve 122. Note that fine grooves having a width of 500 μm or less and a depth of 500 μm or less may be formed on the inner wall of at least one of the first cylindrical portion 11 and the second cylindrical portion 12, or a surface treatment such as roughening may be performed. This reduces the frictional resistance between the filter member 12 or the fatty tissue present inside the first cylindrical portion 11 or the second cylindrical portion 12 and the inner walls of the first cylindrical portion 11 or the second cylindrical portion 12, thereby improving so-called sliding.

[0012] The third cylindrical portion 13 has a bottomed cylindrical shape and an inner diameter shorter than that of the second cylindrical portion 12. The third cylindrical portion 13 is connected to the other end of the second cylindrical portion 12 opposite the end of the second cylindrical portion 12 that is adjacent to the first cylindrical portion 11 in the direction of the cylindrical axis J1, i.e., the end on the -Z direction side, via a connecting portion 15. The connecting portion 15 is cylindrical, connected to the second cylindrical portion 12 at its end on the +Z direction side and to the third cylindrical portion 13 at its end on the -Z direction side, and has a shape that expands in diameter in the +Z direction. The third cylindrical portion 13 further has an outlet 131 provided in its bottom wall 13a for discharging liquid present inside to the outside, and a collection port 132 provided in its side wall 13b for removing fatty tissue present inside. The outlet 131 is provided with a drain cock 133, and the collection port 132 is provided with a collection port opening / closing valve 134. Here, the discharge port 131 and the recovery port 132 may be the same and may be separated outside the third cylindrical portion 13 .

[0013] The gas supply pipe 31 has both ends connected to the first cylindrical portion 11 and the second cylindrical portion 12, and is provided with a gas inlet 32 ​​at approximately the center in the extending direction thereof for introducing gas into the gas supply pipe 31. Gas supply port opening / closing valves 311 and 312 are provided at both ends of the gas supply pipe 31, respectively.

[0014] The filter member 21 is disk-shaped and movable in the cylindrical axis direction inside the cylindrical member 10. When disposed inside the second cylindrical portion 12, the filter member 21 abuts the inner wall of the second cylindrical portion 12. When disposed inside the first cylindrical portion 11, the filter member 21 is sized so that a gap is formed between the peripheral portion and the inner wall of the first cylindrical portion 11. When disposed inside the second cylindrical portion 12, the filter member 21 is disposed in a position that divides the interior of the cylindrical member 10 into two adjacent regions along the cylindrical axis J1. The operating rod 22 is long and has the filter member 21 fixed to one end in the longitudinal direction, and the other end is disposed outside the cylindrical member 10 while being inserted through a through-hole 11b provided in the bottom wall 11a of the first cylindrical portion 11 of the cylindrical member 10. The operating rod 22 can be rotated around the cylindrical axis J1 as indicated by arrow AR11 and moved up and down along the cylindrical axis J1 as indicated by arrow AR12. A disk-shaped portion 23 is provided at the other end of the operating rod 22. Furthermore, a pressing portion 24 that protrudes in the −Z direction and presses fatty tissue present on the −Z direction side of the filter member 21 toward the third cylindrical portion 13 is provided on the side of the filter member 21 opposite to the side to which the operating rod 22 is fixed.

[0015] Next, a method of using the fat separation and purification module 1 according to this embodiment will be described. The inlet 111 of the tubular member 10 of the fat separation and purification module 1 is connected to, for example, a liposuction cannula (not shown) via a tube for transferring adipose tissue (not shown). The exhaust port 112 of the tubular member 10 is connected to a vacuum pump (not shown) via an exhaust pipe (not shown). The inlet 121 of the tubular member 10 is connected to a cleaning fluid supply source (not shown) that supplies cleaning fluid for washing adipose tissue via a tube for transferring cleaning fluid (not shown). The cleaning fluid is, for example, physiological saline. The outlet 131 of the tubular member 10 is connected to a pump (not shown) for suctioning waste fluid via a tube for transferring waste fluid (not shown). The gas inlet 32 ​​is connected to a gas supply source (not shown) that supplies gases such as inert gas and air via a gas supply tube (not shown). This is essentially a general utility. It is also possible to separately perform adipose tissue suction and then introduce the collected adipose tissue via an inlet or the like provided in this device.

[0016] First, the filter member 21 is positioned inside the second cylindrical portion 12 of the cylindrical member 10 using the operating rod 22, i.e., in the region A1 shown in FIG. 2(A). Preferably, the filter member 21 is positioned below the inlet 121. The inlet opening / closing valve 113 and the exhaust port opening / closing valve 114 are both opened, the gas supply port opening / closing valves 311 and 312 are closed, the inlet opening / closing valve 122 is closed, the drain cock 133 is closed, and the recovery port opening / closing valve 134 is closed. Then, with the cannula inserted into the adipose tissue collection target, the vacuum pump is operated, and the gas inside the cylindrical member 10 is discharged, as indicated by arrow AR22 in FIG. 2(A), and the pressure inside the cylindrical member 10 is reduced. As a result, the adipose tissue FA transferred from the collection target through the tube is introduced into the region A2 inside the cylindrical member 10 on the +Z direction side of the filter member 21, as indicated by arrow AR21. That is, by exhausting the gas present inside the tubular member 10 through the exhaust port 112, the inside of the tubular member 10 is made to have a negative pressure relative to the outside of the tubular member 10, and the fatty tissue is sucked into the inside of the tubular member 10 through the inlet 111. Furthermore, the blood or tumescent fluid contained in the fatty tissue FA that has been injected into the +Z direction side of the filter member 21, i.e., a solution of physiological saline with adrenaline added, passes through the filter member 21 and flows out to the -Z direction side of the filter member 21, as shown by arrow AR23.

[0017] Next, after both the inlet opening / closing valve 113 and the exhaust opening / closing valve 114 are closed, the gas supply opening / closing valves 311 and 312 and the drain cock 133 are opened. As a result, as shown by arrow AR25 in FIG. 2(B), gas supplied from the gas supply source is introduced into the cylindrical member 10, and the pressure inside the cylindrical member 10 becomes a preset pressure. Here, the preset pressure is, for example, atmospheric pressure. Furthermore, blood or tumescent fluid contained in the adipose tissue FA that has flowed out to the −Z direction side of the filter member 21, i.e., a solution in which adrenaline has been added to physiological saline, is sucked into a waste fluid suction pump through the outlet 131, as shown by arrow AR24.

[0018] Next, after closing the gas supply port opening / closing valves 311 and 312 and the drain cock 133, the inlet opening / closing valve 122 is opened to introduce the cleaning liquid supplied from the cleaning liquid supply source into the cylindrical member 10. Here, as shown in FIG. 3, the discharge axis J2 of the cleaning liquid discharged from the inlet 121 is shifted in the −X direction from the cylindrical axis J1. As a result, the cleaning liquid discharged from the inlet 121 as indicated by arrow AR26 flows in a vortex shape along the inner wall of the cylindrical member 10 as indicated by arrow AR27. This achieves the effect of stirring the mixture of the adipose tissue FA and the cleaning liquid. Thereafter, after the inlet opening / closing valve 122 is closed, the filter member 21 is moved by the operating rod 22 to introduce cleaning liquid into the area A1 inside the second cylindrical portion 12, as shown by the arrow AR29 in Fig. 4(A), and then the filter member 21 is lifted and returned to the lower part of the inlet 121. The series of steps of introducing cleaning liquid is repeated again, thereby washing the adipose tissue FA. Here, when the filter member 21 is lifted, a pressure difference occurs between both sides of the filter member 21 in the Z-axis direction, and pressure is applied to the adipose tissue FA present on the +Z-direction side of the filter member 21. This pressure is used to release impurities contained in the adipose tissue FA into the cleaning liquid Li.

[0019] Thereafter, the gas supply port opening / closing valve 311, 312, which is provided at the end of the gas supply pipe 31 connected to the first cylindrical portion 11, and the drain cock 133 are opened to allow the cleaning liquid Li mixed with impurities present in the cylindrical portion 10 to be sucked into the waste liquid suction pump through the discharge port 131. Next, after the gas supply port opening / closing valve 311 and the drain cock 133 are closed, the filter member 21 is moved to the region A2 inside the first cylindrical portion 11 by the operating rod 22, as shown by the arrow AR30 in FIG. 4(B). At this time, a gap is formed between the periphery of the filter member 21 and the inner wall of the first cylindrical portion 11, and the filter member 21 comes into proximity with the end of the blade 41 provided on the bottom wall 11a of the first cylindrical portion 11 on the -Z direction side. Next, as shown by arrow AR31 in FIG. 5(A), the filter member 21 is rotated by the operating rod 22, causing the adipose tissue FA present on the +Z direction side of the filter member 21 to fall from the gap between the periphery of the filter member 21 and the inner wall of the first cylindrical portion 11 to the −Z direction side of the filter member 21, as shown by arrow AR32. Here, as shown in FIG. 5(B), the blade 41 has a curved shape and guides the adipose tissue FA present on the +Z direction side of the filter member 21 into the gap between the periphery of the filter member 21 and the inner wall of the first cylindrical portion 11, as shown by arrow AR33. This causes the adipose tissue FA present on the +Z direction side of the filter member 21 to accumulate inside the third cylindrical portion 13. In this case, the blade 41 is not necessarily required; it is also possible to shake off the adipose tissue FA by rotating the filter member 21. Other combinations are also useful.

[0020] 6, syringe 90 is attached to collection port 132. Here, syringe 90 has a long, cylindrical syringe body 901, a plunger 902 that is movable inside syringe body 901 in the axial direction of syringe body 901, and an operation unit 903 connected to plunger 902. The tip of syringe body 901 is attached to collection port 132 in a manner that communicates with collection port 132. Next, after collection port open / close valve 134 is set to an open state, filter member 21 is lowered in the −Z direction by operation rod 22 as shown by arrow AR34 to bring pressing unit 24 into contact with adipose tissue FA, and then, while pressing adipose tissue FA with pressing unit 24, plunger 902 of syringe 90 is moved in a direction to be pulled out of syringe body 901, thereby collecting adipose tissue FA into syringe body 901 as shown by arrow AR35.

[0021] As described above, in the fat separation and purification module 1 according to the present embodiment, when the filter member 21 is placed inside the second cylindrical portion 12 of the cylindrical member 10 using the operating rod 22 and adipose tissue FA and washing liquid Li are introduced into the first cylindrical portion 11 side of the filter member 21 inside the cylindrical member 10, the liquid components contained in the adipose tissue FA and the washing liquid pass through the filter member 21 inside the cylindrical member 10 and move toward the third cylindrical portion 13 side, and the adipose tissue from which the liquid components have been removed remains in the region of the filter member 21 on the first cylindrical portion 11 side inside the cylindrical member 10. Thereafter, by moving the filter member 21 inside the first cylindrical portion 11 using the operating rod 22 and rotating it around the cylindrical axis J1, the adipose tissue FA from which the liquid components present on the +Z direction side of the filter member 21 have been removed moves toward the −Z direction side of the filter member 21 through the gap between the circumferential portion of the filter member 21 and the inner wall of the first cylindrical portion 11. This allows the adipose tissue FA from which the liquid components have been removed to be placed within the third tubular portion 13 while reducing the load on the adipose tissue FA, thereby reducing damage to the adipose tissue FA while removing unnecessary liquid components contained in the adipose tissue FA.

[0022] Adipose tissue injection or transplantation generally consists of three steps: a liposuction step, a fat separation and purification step, and a fat injection step. In the first step, a suction cannula is used to extract fat from the patient's abdomen, thigh, or other areas. Generally, to reduce bleeding, a tumescent solution (i.e., a solution of saline with adrenaline added) is first injected into the patient's area to be sampled. Then, with negative pressure applied to the inside of the suction cannula, the suction cannula is inserted into the area to be sampled and the adipose tissue is aspirated and collected. The collected adipose tissue is a mixture of blood and tumescent solution. In the subsequent fat separation and purification step, the collected adipose tissue is purified by separating and washing the oil components, blood, and other water-soluble components contained therein. In the subsequent fat injection step, the purified adipose tissue is transferred to a syringe and then injected into the area to be sampled using an injection cannula attached to the tip of the syringe. The following methods are commonly used in the fat separation and purification step: One method involves washing the collected adipose tissue with saline and then separating the adipose tissue using a filter. Another method involves mixing the adipose tissue and lactated Ringer's solution in a bag containing a filter. Another method involves transferring the collected adipose tissue to a container such as a syringe body and leaving it to stand, separating the adipose tissue by utilizing the specific gravity of the adipose tissue and liquid components. The separated adipose tissue is then transferred back to the container, and saline is added and left to stand, separating the adipose tissue again. This series of processes is then repeated to remove impurities such as blood from the collected adipose tissue. In this method, the separation of the adipose tissue and liquid components can also be performed using a centrifuge. In this case, a weighted filter member can be placed on the adipose tissue while applying pressure to the adipose tissue.

[0023] In autologous adipose tissue transplantation, the engraftment rate of the adipose tissue is important. While the engraftment rate depends largely on the patient's condition and characteristics, it is believed to depend primarily on the adipose tissue separation and purification method used in the fat separation and purification process. Specifically, it is believed that the engraftment rate will decrease if, during the process of separating and purifying the collected adipose tissue, unnecessary liquid components such as water, blood components, and oil are not sufficiently separated from the adipose tissue, or if excessive physical stress is applied to the adipose tissue during washing, causing damage to the adipose tissue. In this regard, the aforementioned method of separating the collected adipose tissue by leaving it stationary may result in insufficient separation of the liquid components contained in the collected adipose tissue. Furthermore, using the aforementioned centrifuge may result in excessive physical stress being applied to the adipose tissue, causing damage to the adipose tissue. Furthermore, even the aforementioned method of separating and purifying adipose tissue using a filter member may result in insufficient separation of the adipose tissue.

[0024] In contrast, the fat separation and purification module 1 according to this embodiment can sufficiently remove liquid components such as blood components and oil components that are thought to affect the engraftment rate of adipose tissue from the adipose tissue, and can also suppress damage to the adipose tissue. Another advantage is that it can achieve a degree of washing appropriate for the patient without excessive separation. As an example, it is possible to provide adipose tissue that has absorbed a certain amount of washing liquid (adipose tissue with adjusted fluidity). This can be achieved by adjusting the number of washings, etc.

[0025] (Embodiment 2) The fat separation and purification device of this embodiment comprises the fat separation and purification module 1 described in embodiment 1, a support stand that supports the fat separation and purification module 1, a rotation handle that is arranged on the outside of the tubular member 10 and that rotates the filter member 21 around the tubular axis J1 of the tubular member 10 via the operating rod 22, and a movement handle that is arranged on the outside of the tubular member 10 and that moves the filter member 21 in the direction of the tubular axis J1 of the tubular member 10 via the operating rod 22.

[0026] As shown in FIG. 7 , the fat separation and purification device according to this embodiment includes a support base 50 that supports the fat separation and purification module 1 and a drive unit 60 for manually driving the filter member 21. In FIG. 7 , the same components as those in the first embodiment are denoted by the same reference numerals as those in FIG. 1. The support base 50 includes a plurality of support columns 51, a plate-like tubular member support plate 52 that has a circular through-hole 52a in a plan view formed in its central portion and through which a portion of the tubular member 10 of the fat separation and purification module 1 is inserted, and whose peripheral portion is supported by the support columns 51, and a drive unit support plate 53 that is mounted on the tip of the support column 51 and supports the drive unit 60. The inner diameter of the through-hole 52a is shorter than the outer diameter of the second tubular portion 12 and longer than the outer diameter of the third tubular portion 13. Thus, the tubular member support plate 52 supports the tubular member 10 with the outer periphery of the through-hole 52a abutting against the side wall of the connecting portion 15 of the tubular member 10 inserted through the through-hole 52a. The support base 50 also has a plurality of tubular member holding portions 54 that abut against the end portion of the tubular member 10 on the +Z direction side and hold the tubular member 10 by sandwiching the tubular member 10 between the tubular member holding plate 52 and the tubular member 10 in the Z axis direction. The tubular member holding portions 54 are attached to the support columns 51 so as to be movable in a direction perpendicular to the Z axis direction relative to the support columns 51, as shown by arrow AR2011. The tubular member holding portions 54 are movable between a holding position where they abut against the end portion of the tubular member 10 on the +Z direction side and hold the tubular member 10, and a standby position where they are standby outside the projection area of ​​the tubular member 10 on the +Z direction side.

[0027] The drive unit 60 includes a slider 61 that is movable in the Z-axis direction together with the operating rod 22, a gear 62, and a movement handle 63 that is operated to move the operating rod 22 and slider 61 in the Z-axis direction. The slider 61 includes a rotor 612 having a columnar locking portion 611 to which the disk-shaped portion 23 at the end of the operating rod 22 on the +Z direction side is fixed, and a rotor support portion 613 that rotatably supports the rotor 612. The locking portion 611 has a so-called ball lock structure in which the disk-shaped portion 23 is fixed with a single touch by, for example, pressing the disk-shaped portion 23 against the locking portion 611. The gear 62 is fixed to the rotor 612 of the slider 61. The movement handle 63 is connected to the rotor support portion 613 of the slider 61. Furthermore, drive unit 60 is supported by drive unit support plate 53 on the +Z direction side of drive unit support plate 53 so as to be rotatable about rotation axis J3 and includes a spline shaft 64 that meshes with gear 62, and a rotation handle 65 that is connected to the end of spline shaft 64 on the +Z direction side and is operated to rotate operating rod 22 and rotating body 612 of slider 61 about rotation axis J3. An operating rod cover (not shown) may be provided to cover the outside of operating rod 22 and keep operating rod 22 clean.

[0028] In the fat separating and purification device according to this embodiment, the support base 50 and drive unit 60 are used repeatedly multiple times, and the fat separating and purification module 1 is replaced for each patient. The portion of the fat separating and purification module 1 that comes into contact with the adipose tissue inside the tubular member 10 is sterilized, and the inside of the tubular member 10 is designed to be isolated from the outside air by closing the inlet opening / closing valve 113, the exhaust port opening / closing valve 114, the introduction port opening / closing valve 122, the drain cock 133, and the gas supply port opening / closing valves 311 and 312.

[0029] As described above, according to the fat separation and purification device of this embodiment, as in embodiment 1, the adipose tissue FA from which liquid components have been removed can be placed inside the third tubular portion 13 while reducing the load on the adipose tissue FA, thereby reducing damage to the adipose tissue FA while removing unnecessary liquid components contained in the adipose tissue FA.

[0030] Although the embodiments of the present invention have been described above, the present invention is not limited to the configurations of the above-described embodiments. For example, as in the fat separation and purification device shown in Fig. 8, a fat separation and purification device may be provided with a base member 2071 on which the fat separation and purification module 1 is placed, a syringe holding part 2072 that is provided on the +Z direction side of the base member 2071 and holds the syringe 90, and a rotation lock mechanism 2073 that prevents the operation part 903 of the syringe 90 from rotating.

[0031] In the second embodiment, an example has been described in which a drive unit 60 is provided for a user to manually drive the filter member 21. However, this is not limiting, and the filter member 21 may be driven automatically. As shown in Fig. 9 , the fat separating and purifying device according to this modification includes a fat separating and purifying module 1, a support stand 50, a base member 3071 to which the support stand 50 is fixed, a rotational drive unit 3080 for rotationally driving the filter member 21, an axial drive unit 3090 for driving the filter member 21 up and down in the Z-axis direction, and a syringe drive unit 3100 for driving the syringe 90. The rotational drive unit 3080 includes a motor 3081, a fixing part 3082 connected to the tip of the shaft of the motor 3081 and to which the disc-shaped part 23 is fixed, and a bearing 3083 whose outer ring is fixed to a lifting plate 3095 (described later) and whose inner ring is fixed to the operating rod 22.

[0032] The axial drive unit 3090 has a lifting plate 3095, a motor 3091, a long sliding screw 3093 fixed to the shaft of the motor 3091 via a fixing part 3092, and a nut 3094 fixed to the lifting plate 3095, engaged with the sliding screw 3093, and moving together with the lifting plate 3095 along the sliding screw 3093 as the sliding screw 3093 rotates.

[0033] The syringe drive unit 3100 includes a long transmission rod 3103 having a chuck 3104 at its tip end for holding the operation part 903 of the syringe 90, and an actuator 3101 for moving a fixing part 3102 to which the transmission rod 3103 is fixed in the Y-axis direction. The fat separating and refining apparatus also includes a valve opening / closing control part (not shown) for controlling the opening and closing of the inlet opening / closing valve 113, the exhaust port opening / closing valve 114, the gas supply port opening / closing valves 311 and 312, the drain cock 133, the inlet opening / closing valve 122, and the recovery port opening / closing valve 134. The fat separating and refining apparatus also includes a cleaning status determination part (not shown) for determining the cleaning status of the adipose tissue. This cleaning status determination part may determine whether cleaning is complete in response to a user's operation, or may include a colorimeter for measuring the color of the adipose tissue and determine whether cleaning is complete based on the color measured by the colorimeter.

[0034] Next, the fat separation and purification process performed by the fat separation and purification device according to this modified example will be described with reference to Fig. 10. First, the axial direction drive unit 3090 positions the filter member 21 in the inner area A1 of the cylindrical member 10 via the operating rod 22. Then, the valve opening / closing control unit opens the input port opening / closing valve 113 and the exhaust port opening / closing valve 114, and closes the gas supply port opening / closing valves 311 and 312, the inlet opening / closing valve 122, the drain cock 133, and the recovery port opening / closing valve 134. Then, with the cannula inserted into the adipose tissue collection target, the vacuum pump is operated to reduce the pressure inside the cylindrical member 10, and the adipose tissue FA is sucked from the collection target and introduced into the +Z direction side of the filter member 21 within the cylindrical member 10 (step S101).

[0035] Next, the valve opening / closing control unit closes both the inlet opening / closing valve 113 and the exhaust port opening / closing valve 114, and then opens the gas supply port opening / closing valves 311, 312 and the drain cock 133 (step S102). As a result, the blood or tumescent fluid contained in the adipose tissue FA present on the +Z direction side of the filter member 21, i.e., a solution in which adrenaline has been added to physiological saline, passes through the filter member 21 and flows out to the -Z direction side of the filter member 21, and is sucked into the waste liquid suction pump through the exhaust port 131.

[0036] Next, the valve opening / closing control unit closes the gas supply port opening / closing valves 311, 312 and the drain cock 133, opens the inlet opening / closing valve 122, and introduces the cleaning liquid supplied from the cleaning liquid supply source into the cylindrical member 10 (step S103). After that, the valve opening / closing control unit closes the inlet opening / closing valve 122, and then the axial direction drive unit 3090 introduces the cleaning liquid into the filter member 21 in the inner region A1 of the second cylindrical member 12 via the operating rod 22 and lifts the filter member 21. If the cleaning is insufficient, the filter member 21 is returned to the lower part of the inlet 121, and the introduction of the cleaning liquid is repeated again to wash the adipose tissue FA (step S104).

[0037] Next, when a preset time has elapsed since the start of cleaning, the axial direction drive unit 3090 stops driving the filter member 21. Then, the cleaning state determination unit determines whether or not cleaning has been completed (step S105). If it is determined that cleaning has not been completed yet (step S105: No), the process of step S104 is executed again.

[0038] On the other hand, when it is determined that the cleaning is completed (step S105: Yes), the valve opening / closing control unit opens the gas supply port opening / closing valve 311, 312, which is provided at the end of the gas supply pipe 31 connected to the first cylindrical portion 11, and the drain cock 133 (step S106). Thereafter, the axial direction drive unit 3090 raises the filter member 21 to the region A2 via the operating rod 22 (step S107). Next, the rotation drive unit 3080 rotates the filter member 21 via the operating rod 22 (step S108). As a result, the adipose tissue FA present on the +Z direction side of the filter member 21 falls from the gap between the circumferential portion of the filter member 21 and the inner wall of the first cylindrical portion 11 to the −Z direction side of the filter member 21 and accumulates inside the third cylindrical portion 13.

[0039] Next, the valve opening / closing control unit closes the gas supply port opening / closing valve 311 and the drain cock 133, and opens the recovery port opening / closing valve 134 (step S109). After that, the axial direction drive unit 3090 lowers the filter member 21 via the operating rod 22, causing the pressing portion 24 to abut against the adipose tissue FA. Then, while the axial direction drive unit 3090 lowers the filter member 21 to press the adipose tissue FA with the pressing portion 24, the syringe drive unit 3100 moves the plunger 902 of the syringe 90 in a direction to pull it out of the syringe body 901, thereby recovering the adipose tissue FA into the syringe body 901 (step S110).

[0040] According to this configuration, the user does not need to drive the filter member 21 or operate the inlet opening / closing valve 113, the exhaust port opening / closing valve 114, the gas supply port opening / closing valves 311, 312, the drain cock 133, the inlet opening / closing valve 122, and the recovery port opening / closing valve 134, thereby improving convenience for the user.

[0041] In each embodiment, an example has been described in which one syringe 90 is attached to the third tubular portion 13 of the tubular member 10, but this is not limited to this, and for example, the third tubular portion 13 may be provided with multiple recovery ports 132 to which multiple syringes 90 are each attached.

[0042] In embodiment 1, the fat separation and purification module 1 may be arranged outside the tubular member 10, fixed to the disk-shaped portion 23 at the other end of the operating rod 22, and equipped with a handle (not shown) for rotating the filter member 21 around the tubular axis J1 of the tubular member 10 or moving it in the direction of the tubular axis J1 of the tubular member 10 via the operating rod 22.

[0043] Although the embodiments and modifications of the present invention have been described above, the present invention is not limited to these. The present invention includes any combination of the embodiments and modifications, and any combination to which appropriate modifications have been made. [Industrial Applicability]

[0044] The present invention is suitable as a fat separation and purification device for separating and purifying adipose tissue to be used for adipose tissue injection and adipose tissue transplantation. [Explanation of symbols]

[0045] 1: fat separation and purification module, 10: cylindrical member, 11: first cylindrical portion, 11a, 13a: bottom wall, 11b, 52a: through-hole, 12: second cylindrical portion, 13: third cylindrical portion, 13b: side wall, 14, 15: connection portion, 21: filter member, 22: operation rod, 23: disc-shaped portion, 24: pressing portion, 31: gas supply pipe, 32: gas inlet port, 41: blade, 50: support base, 51: support column, 52: cylindrical member support plate, 53: drive unit support plate, 54: cylindrical member holding portion, 60: drive unit, 61: slider, 62: gear, 63: moving handle, 64: spline shaft, 65: rotating handle, 90: syringe, 111: input port, 112: exhaust port, 113 : Inlet opening / closing valve, 114: Exhaust port opening / closing valve, 121: Inlet, 122: Inlet opening / closing valve, 131: Outlet, 132: Recovery port, 133: Drain cock, 134: Recovery port opening / closing valve, 311, 312: Gas supply port opening / closing valve, 611: Locking portion, 612: Rotating body, 613: Rotating body support portion, 901: Syringe body, 902: Plunger, 903: Operating portion, 2071, 3071: Base member, 2072: Syringe holding portion, 2073: Rotation lock mechanism, 3080: Rotation drive unit, 3082, 3092, 3102: Fixing portion, 3090: Axial drive unit, 3100: Syringe drive unit, FA: Adipose tissue, Li: Cleaning solution

Claims

1. A fat separation and purification module for separating and purifying liquid components from adipose tissue, a cylindrical member having: a first cylindrical portion having a bottom and a through hole formed in the bottom wall; a second cylindrical portion having a cylindrical shape and a cross-sectional area perpendicular to the cylindrical axis direction that is smaller than the cross-sectional area perpendicular to the cylindrical axis direction of the first cylindrical portion, the second cylindrical portion being continuous with one end of the first cylindrical portion in the cylindrical axis direction opposite to the one end on the bottom wall side of the first cylindrical portion, and having an inlet for introducing a cleaning solution for purifying adipose tissue inside; and a third cylindrical portion being continuous with the other end of the second cylindrical portion in the cylindrical axis direction opposite to the one end on the first cylindrical portion side of the second cylindrical portion, and having an outlet for discharging liquid present inside to the outside; a filter member that is movable in the cylindrical member in the cylindrical axis direction, has a size and shape such that when disposed inside the second cylindrical portion, its peripheral portion abuts against the inner wall of the second cylindrical portion, and when disposed inside the first cylindrical portion, a gap is formed between the peripheral portion and the inner wall of the first cylindrical portion, and is disposed inside the second cylindrical portion in an orientation that divides the interior of the cylindrical member into two regions adjacent to each other in the cylindrical axis direction; an operating rod having a long length, one end of which is fixed to the filter member in the longitudinal direction, and the other end of which is inserted into the through hole and disposed outside the cylindrical member; Fat separation and purification module.

2. a handle disposed on the outside of the cylindrical member for rotating the filter member around the cylindrical axis of the cylindrical member and for moving the filter member in the cylindrical axis direction of the cylindrical member via the operating rod; The fat separation and purification module according to claim 1.

3. a blade provided on a bottom wall inside the first cylindrical portion, the blade guiding the fatty tissue adhering to the bottom wall side of the filter member to the gap between the circumferential portion of the filter member and the inner wall of the cylindrical portion by rotating the filter member while the filter member is in contact with the bottom wall inside the first cylindrical portion. The fat separation and purification module according to claim 1 or 2.

4. a pressing portion provided on the opposite side of the filter member from the side to which the operating rod is fixed, protruding toward the third cylindrical portion and pressing the fatty tissue present on the third cylindrical portion side of the filter member toward the third cylindrical portion, The third cylindrical portion further has a collection port for removing the adipose tissue present inside the third cylindrical portion. The fat separation and purification module according to claim 1 or 2.

5. The first cylindrical portion further has an inlet for introducing the fatty tissue therein and an exhaust port for discharging gas present therein, The gas present inside the cylindrical member is exhausted through the exhaust port, thereby creating a negative pressure inside the cylindrical member relative to the outside of the cylindrical member, and the fatty tissue is then sucked into the cylindrical member from the inlet. The fat separation and purification module according to claim 1 or 2.

6. a fat separation and purification module for separating and purifying liquid components from the fat tissue; a support base for supporting the fat separation and purification module; a drive unit fixed to the support base and configured to drive the fat separation and purification module; The fat separation and purification module comprises: a cylindrical member having: a first cylindrical portion having a bottom and a through hole formed in the bottom wall; a second cylindrical portion having a cylindrical shape and a cross-sectional area perpendicular to the cylindrical axis direction that is smaller than the cross-sectional area perpendicular to the cylindrical axis direction of the first cylindrical portion, the second cylindrical portion being continuous with one end of the first cylindrical portion in the cylindrical axis direction opposite to the one end on the bottom wall side of the first cylindrical portion, and having an inlet for introducing a cleaning solution for purifying adipose tissue inside; and a third cylindrical portion being continuous with the other end of the second cylindrical portion in the cylindrical axis direction opposite to the one end on the first cylindrical portion side of the second cylindrical portion, and having an outlet for discharging liquid present inside to the outside; a filter member that is movable in the cylindrical member in the cylindrical axis direction, has a size and shape such that when disposed inside the second cylindrical portion, its peripheral portion abuts against the inner wall of the second cylindrical portion, and when disposed inside the first cylindrical portion, a gap is formed between the peripheral portion and the inner wall of the first cylindrical portion, and is disposed inside the second cylindrical portion in an orientation that divides the interior of the cylindrical member into two regions adjacent to each other in the cylindrical axis direction; an operating rod having a long length, one end of which is fixed to the filter member in the longitudinal direction, and the other end of which is inserted into the through hole and disposed outside the cylindrical member; The drive unit is a rotation handle disposed outside the cylindrical member for rotating the filter member around the cylindrical axis of the cylindrical member via the operating rod; an axial movement handle disposed outside the cylindrical member for moving the filter member in the axial direction of the cylindrical member via the operating rod, When at least one of the rotation handle and the axial direction movement handle is operated, the filter member is rotated or moved in the cylindrical axis direction via the operating rod. Fat separation and purification equipment.

7. a cylindrical member including: a first cylindrical portion having a bottom and a through-hole formed in the bottom wall; a second cylindrical portion having a cylindrical shape and a cross-sectional area perpendicular to the cylindrical axis direction that is smaller than the cross-sectional area perpendicular to the cylindrical axis direction of the first cylindrical portion, the second cylindrical portion being continuous with one end of the first cylindrical portion in the cylindrical axis direction opposite to the end on the bottom wall side of the first cylindrical portion, and having an inlet for introducing a cleaning solution for purifying adipose tissue inside; and a third cylindrical portion being continuous with the other end of the second cylindrical portion in the cylindrical axis direction opposite to the one end on the first cylindrical portion side of the second cylindrical portion, and having an outlet for discharging a liquid present inside to the outside. a fat separation and purification module for separating and purifying liquid components from the fatty tissue, the module having: a filter member that is movable in a direction perpendicular to the axis of the cylindrical body, and that has a size and shape such that a peripheral portion of the filter member abuts against the inner wall of the second cylindrical portion when the module is disposed inside the second cylindrical portion and that a gap is formed between the peripheral portion and the inner wall of the first cylindrical portion when the module is disposed inside the first cylindrical portion, the filter member being disposed in an orientation such that the interior of the cylindrical member is divided into two regions adjacent to each other in the cylindrical axis direction when the module is disposed inside the second cylindrical portion; and a long operating rod having one end in the longitudinal direction to which the filter member is fixed and the other end of the operating rod being inserted into the through-hole and disposed outside the cylindrical member; an axial drive unit that drives the filter member in the axial direction of the cylindrical member; a rotation drive unit that rotates the filter member around the cylindrical axis of the cylindrical member, the axial direction drive unit separates the liquid component from the fatty tissue by moving the filter member toward the opposite side from the outlet side, while the fatty tissue and the cleaning solution are present in a region inside the tubular member on the opposite side from the outlet side of the filter member. Fat separation and purification equipment.

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