Plant vein absorption method

Abstract

To provide a plant vein absorption method, and to provide a functional material for plant modification.SOLUTION: Provided are a plant vein absorption method and a functional material for plant modification, comprising a covering body having a functional material and a protective body having a polymerized stabilizing material. The functional material is a mono-, poly- or high-entropy compound, a semiconductor or a photoelectric material. The covering body covers the surface of a plant organ and contains a stomatal expansion material so that the functional material is absorbed into the plant through the stomata. The protective body covers the surface of the covering body. The functional material can functionally exist on the plant surface until the plant organ naturally undergoes apoptosis. The plant surface vein absorption technology can achieve special functions without modifying or implanting plant genes. In addition to providing a new experience of appreciating plants at night, carbon sequestration capabilities such as carbon capture and carbon neutrality can also be achieved at the same time.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention relates to a plant surface modification product and a plant absorption method, and more particularly to a plant leaf vein absorption method and a functional material for plant modification. [Background technology]

[0002] Efficient agricultural production has great potential in the application of agricultural and horticultural science and technology around the world. However, plant surface modification techniques are still in their infancy in the horticulture and biotechnology industries. Therefore, there is a plant surface modification technology that can impart special functionality to horticulture and agriculture by applying functional materials to plants. However, current plant surface modification techniques often involve covering the plant surface, which can lead to a decline in plant surface functionality and even death. Functional materials cannot remain on the plant surface for a relatively long period of time. If plant genes are modified or introduced using biological methods, special functions can be achieved, but problems such as gene contamination are likely to occur and many restrictions are also imposed. Summary of the Invention [Problem to be solved by the invention]

[0003] SUMMARY OF THE INVENTION An object of the present invention is to provide a plant vein absorption method and a functional material for plant modification in order to solve the above-mentioned problems of the prior art.

[0004] The present invention aims to devise a completely new plant leaf vein absorption technology that can effectively generate special physical or chemical functions by absorbing single-component, multi-component, or high-entropy compounds, semiconductors, or photoelectric materials, and that allows plants to grow naturally even after modification, can be widely and effectively applied to natural plants, and can effectively promote the progress of horticulture and agriculture using functional plants. In addition, the ability to fix the carbon dioxide generated at night is in line with international net-zero carbon emissions policies, achieving carbon neutrality. [Means for solving the problem]

[0005] In order to achieve the above object, the present invention provides: A coating body having a functional material and a protective body having a polymerization stabilizing material. The coating is intended to cover the surface of a plant organ, and a functional material for plant modification is proposed, which further contains a pore expander, such as fusicoccin, so that the functional material can be absorbed into the plant through the pores of the plant organ.

[0006] The plant leaf vein absorption method of the present invention comprises: providing a coating having a functional material, the coating being for covering a surface of a plant organ and including a pore expander for promoting expansion of a plurality of pores on the surface of the plant organ so that the functional material can be absorbed into the plant through the pores of the plant organ, the coating being an aqueous medium having the adhesive properties of a bioglue or a biodegradable glue and an adjustable surface charge; and (b) providing a protective body having a polymerized stabilizing material, the protective body being for covering the surface of the coated body and being another aqueous substrate having the bioglue or the biodegradable glue, the polymerized stabilizing material stabilizing and polymerizing the bioglue or the biodegradable glue of the protective body and / or the coated body, thereby increasing the amount and density of absorption of the functional material by the plant organ.

[0007] The plant organ is a leaf or a modified leaf.

[0008] The covering material is sprayed, painted or coated on the plant organ, and the protective material is sprayed, painted or coated on the covering material.

[0009] The pore expander is fusicoccin, which functions to promote the expansion of the stomata on the surface of the plant organ.

[0010] The content of the pore expander in the coating is 170 μM or less.

[0011] The particle size of the functional material is 2 μm to 5 nm.

[0012] The functional material is a nanomaterial, a sub-nanomaterial, or a micro-nanomaterial.

[0013] The zeta potential due to the surface charge of the functional material is +0 to +75 meV.

[0014] The polymeric stabilizing material of the protective body is alum ions, which polymerize and stabilize the bioglue or biodegradable glue.

[0015] The weight percentage of the polymer stabilizing material in the protective body is 35% or less.

[0016] The bioglue is soft deer glue, hard deer glue, swimbladder glue, cow glue, rabbit glue, seedlac and / or Sansenbon glue, and the biodegradable glue is polyamide, polyvinyl pyrrolidone and / or polyvinyl acetate.

[0017] The aqueous base of the coating is an acidic aqueous base, and the acidic aqueous base is an aqueous solvent containing citric acid, succinic acid, tannic acid, salicylic acid, malic acid, ascorbic acid, gallic acid, hydrochloric acid, nitric acid, and / or acetic acid.

[0018] The aqueous base material of the coating is an alkaline aqueous base material, and the alkaline aqueous base material is an aqueous solvent containing sodium bicarbonate, sodium carbonate, sodium hydroxide, potassium bicarbonate, potassium carbonate, and / or potassium hydroxide.

[0019] The functional material is needle-shaped or sea urchin-shaped gold nanoparticles.

[0020] In order to achieve the above-mentioned object, the present invention proposes a plant leaf vein absorption method, which includes at least the steps of producing a coating having a functional material and producing a protective body having a polymerized stabilizing material, wherein the coating is for covering the surface of a plant organ, and further contains a pore expander, such as fusicoccin, so that the functional material is absorbed into the plant through the stomata of the plant organ, and the plant organ is a leaf or a modified leaf.

[0021] The plant leaf vein absorption method of the present invention comprises: A step of mixing a functional material suspension solvent containing a functional material with an aqueous solvent using bioglue or biodegradable glue to form a viscous functional material suspension solvent; mixing the adhesive functional material suspension solvent with an acidic or alkaline aqueous solvent to form an adhesive functional material suspension having an adjustable surface charge as a coating having a functional material; A step of covering a surface of a plant organ with the covering body, wherein the functional material suspension medium containing the functional material further contains a pore expander for promoting the expansion of a plurality of pores on the surface of the plant organ, and the plant organ is a leaf or a transformed leaf; forming a protective body by mixing a polymeric stabilizing material in a separate aqueous solvent using a bioglue or biodegradable glue; and covering the surface of the covering body with the protective body, wherein the polymerized stabilizing material stabilizes and polymerizes the bioglue or biodegradable glue of the protective body and / or the covering body, thereby increasing the absorption amount and density of the functional material by the plant organ.

[0022] In the plant vein absorption method of the present invention, the bioglue of the protective body and / or the coating body is soft deer glue, hard deer glue, swimbladder glue, cow glue, rabbit glue, seedlac and / or Sansenbon glue, and the biodegradable glue is polyamide, polyvinyl pyrrolidone and / or polyvinyl acetate.

[0023] In the plant leaf vein absorption method according to the present invention, the stomatal expansion material is fusicoccin, and the polymerization stabilizing material is alum ions.

[0024] In the plant leaf vein absorption method according to the present invention, the covering body is sprayed, applied or coated on the plant organ, and the protection body is sprayed, applied or coated on the covering body.

[0025] In the plant leaf vein absorption method according to the present invention, the particle size of the functional material is 2 μm to 5 nm.

[0026] In the plant leaf vein absorption method according to the present invention, the zeta potential due to the surface charge of the functional material is +0 to +75 meV. [Effects of the Invention]

[0027] The plant leaf vein absorption method and the functional material for plant modification of the present invention have the following advantages.

[0028] (1) By using nano-plant leaf vein absorption technology, the functions of the plant are not impaired and the plant can coexist with the plant.

[0029] (2) The functional material can remain functional in the plant until the plant organ undergoes natural apoptosis.

[0030] (3) Through nano-plant vein absorption technology, special control functions can be achieved without modifying or introducing plant genes.

[0031] (4) It complies with the EU safety directives, making it safer to use and favorable for international marketing.

[0032] (5) Luminescent plants enhance the carbon dioxide fixation effect of plants, and are expected to be applied to carbon negativity and food production in the future.

[0033] (6) By combining energy gap engineering units, multi-element or high-entropy compounds, semiconductors, and photoelectric materials, it is possible to effectively adjust the emission spectrum, improve the aesthetic appeal, and enhance the carbon sequestration and physiological growth functions of plants, which may be useful for achieving net-zero carbon emissions and increasing food production.

[0034] In order to better understand the technical features and achievable technical effects of the present invention, better embodiments and detailed descriptions are provided below. [Brief explanation of the drawings]

[0035] [Figure 1] 1 is a schematic diagram of the plant vein absorption method of the present invention and a functional material for plant modification in which a protective body is not covered on a covering body. [Figure 2] 1 is a schematic diagram of a plant vein absorption method of the present invention and a functional material for plant modification in which a protective body is covered on a covering body. [Figure 3] 1 is a schematic diagram of the plant vein absorption method of the present invention and the functional material for plant modification when applied to the surface of a plant organ, which is a leaf or a modified leaf. DETAILED DESCRIPTION OF THE INVENTION

[0036] Hereinafter, embodiments of the present invention will be described with reference to the drawings. The proportions of the components in the drawings of the embodiments of the present invention are shown for ease of understanding and are not actual proportions. Furthermore, the dimensional proportions of the assemblies shown in the drawings are for the purpose of explaining the components and their structures, and the present invention is not limited thereto. Meanwhile, for ease of understanding, the same components in the following embodiments will be described with the same reference numerals.

[0037] Furthermore, terms used throughout the specification and claims generally have their ordinary meanings as used in the art, in the context disclosed herein, and in the particular context, unless otherwise specified. Some terms used to describe the present invention are explained below or elsewhere in this specification to provide those of ordinary skill in the art with additional guidance regarding the description of the present invention.

[0038] Secondly, when this article uses terms such as "including," "comprising," "having," and "containing," they are all open terms, meaning including but not limited to.

[0039] As shown in Figures 1 to 3, Figures 1 and 2 are schematic diagrams of the plant modification functional material of the present invention, and Figure 3 is a schematic diagram of the plant modification functional material of the present invention when applied to the surface of a plant organ. Here, the plant organ 300 shown in Figure 3 is a leaf. The plant modification functional material 1 of the present invention includes a coating body 100 having a functional material 10 and a protection body 200 having a polymerization stabilizing material 20. The functional material can be, for example, a unidimensional, multidimensional or high-entropy compound, a semiconductor or an optoelectronic material. As an example of a high-entropy material, a component of the functional material of the present invention is, for example, a high-entropy oxide or a high-entropy oxide-doped semiconductor, where high entropy means that the element contains at least five elements and has an atomic percentage of 5 to 35. The above is merely an example and does not limit the functional material of the present invention. Energy gap engineering design can be used in semiconductors and photovoltaic materials to effectively tailor the emission spectra of single-, multi- or high-entropy compounds. For example, the functional material used in the present invention can emit blue light in the case of a rare earth element, and can emit blue, green and red light, or a composite light in the case of a transition element. The present invention can adjust the color temperature of the emission spectrum by adjusting the proportions of blue, green, and red light. The plant organ 300 of the plant is, for example, a leaf or a modified leaf, in particular a leaf vein. Here, the plant organ 300 of the above-mentioned plant may optionally be subjected to a pretreatment step. For example, in the present invention, the outer surface of the plant organ 300 may be pretreated by uniformly mixing 6.5 g to 16.5 g of hydrogen peroxide (HP), 2 g to 30 g of polyvinyl pyrrolidone (PVP), 3 g to 9 g of polyvinyl alcohol (PVA), 1,000 Units / mL to 900,000 Units / mL of nystatin, and 550 μL of sulfuric acid (H2SO4, 96%) in 50 g to 500 g of deionized water. The plant surface modified body 1, coating body 100 and protective body 200 of the present invention are not limited to a specific structural form and may be liquid, gel or layer-like, etc., and can be applied to the present invention as long as they can cover the plant organ 300 of the plant. 1 and 2 illustrate a layered covering 100 and a protective covering 200. FIG. If it has a layered structure, the layered structure may be selectively maintained, or it may be crushed into chips or powder so as to cover the plant organ 300 . It is preferable that the covering body 100 and the protecting body 200 are transparent or light-transmitting, and if they are light-transmitting, they preferably retain the primary color of the plant organ. The dimensions of the covering body 100 and the protecting body 200, such as length, width and thickness, or the amount used, are applicable to the present invention as long as they can cover the plant organ 300 of the plant, but are not particularly limited. Furthermore, the properties of the covering body 100 and the protecting body 200, such as density, hardness, or flexibility, can also be applied to the present invention as long as they are capable of covering the plant organ 300 of the plant.

[0040] In the present invention, the covering body 100 can cover the surface 302 of the plant organ 300 of the plant by, for example, spraying, painting, or coating. Here, the coating 100 further contains a pore-expanding material such as fusicoccin, and the content of fusicoccin in the coating 100 is approximately 170 μM or less. Fusicoccin has the function of promoting the expansion of stomata on the surface 302 of the plant organ 300, allowing the stomata to open smoothly, and facilitating the absorption of the functional material 10 into the plant through the plant organ 300, such as the stomata around the veins of the leaves. The coating 100 is a water-based substrate with tunable surface charge that has the adhesive properties of a bioglue or biodegradable glue. Here, the aqueous medium of the present invention is, for example, an aqueous solvent, and may be, for example, an aqueous liquid or an aerosol. Bioglues are, for example, soft deer glue, hard deer glue, swim bladder glue, cow glue, rabbit glue, sheath track and / or 3000 glue. The biodegradable glue may be, for example, polyamide, polyvinyl pyrrolidone, and / or polyvinyl acetate. The weight percentage of bio-glue or biodegradable glue in the covering 100 is preferably about 35% or less. In the present invention, a self-decomposable bio-glue or biodegradable glue is used, and when the functional material 10 is absorbed by the plant, the bio-glue or biodegradable glue covering the surface of the plant organ 300 is decomposed or biodegraded, so as not to inhibit the plant's growth function and cause damage to the plant. The aqueous base of the coating 100 is, for example, an acidic aqueous base or an alkaline aqueous base, and the acidic aqueous base is an aqueous solvent containing citric acid, succinic acid, tannic acid, salicylic acid, malic acid, ascorbic acid, gallic acid, hydrochloric acid, nitric acid, and / or acetic acid. The alkaline aqueous base is an aqueous solvent that includes sodium bicarbonate, sodium carbonate, sodium hydroxide, potassium bicarbonate, potassium carbonate, and / or potassium hydroxide. Thus, the coating 100 of the present invention is a water-based substrate with tunable surface charge that has the adhesive properties of a bioglue or biodegradable glue.

[0041] The functional material 10 of the present invention is not limited to a specific functional material, but may be any material with functionality that exhibits various functions, such as making plants glow without poisoning them or imparting more properties to plants. For example, if the functional material 10 is a needle-shaped or sea urchin-shaped gold nanoparticle, it can serve as an excitation light source and make a plant emit light when irradiated with ultraviolet light. The functional material 10 of the present invention has a particle size that is much smaller than the pore size of the stomata of a plant organ 300 such as a leaf, and the functional material 10 is a nanomaterial, sub-nanomaterial, or micro-nanomaterial, and the particle size of the functional material 10 is, for example, 2 μm to 5 nm. The zeta potential due to the surface charge of the functional material 10 is +0 to +75 meV. The depth to which the functional material 10 penetrates into the plant organ 300 is affected by the zeta potential due to the surface charge, and if the zeta potential of the functional material surface is +5 to +75 meV, it helps the functional material 10 to enter the lattice cells, columnar cells, and even chloroplasts. If the zeta potential of the surface of the functional material 10 is −1 to −65 meV, the functional material 10 will remain on the epidermis of the plant and help protect the cells. On the other hand, many of the prior art plant paints are merely protective agents that cover the surface of plants.

[0042] In the present invention, the protective body 200 may be covered on the surface of the covering body 100 by, for example, spraying, painting, or coating, and the protective body 200 has a polymerized stabilizing material 20 for stabilizing and polymerizing the bioglue or biodegradable glue of the protective body 200 and / or the covering body 100, thereby increasing the absorption amount and density of the functional material 10 by the plant organ 300. The polymeric stabilizing material 20 of the protector 200 is, for example, alum ions that polymerize and stabilize bioglue or biodegradable glue. The weight percentage of the polymeric stabilizing material 20 to the protector 200 is preferably about 35% or less. Here, the protective body 200 is another aqueous base material having the bioglue or biodegradable glue described above, and the weight percentage of the bioglue or biodegradable glue relative to the protective body 200 is preferably about 63% or less. Among them, the bioglue is soft deer glue, hard deer glue, swimbladder glue, cow glue, rabbit glue, granule glue and / or 3000 glue. The biodegradable glue is polyamide, polyvinyl pyrrolidone and / or polyvinyl acetate.

[0043] Here, the other aqueous base material of the protector 200 is, for example, an acidic aqueous base material or an alkaline aqueous base material, and the acidic aqueous base material is an aqueous solvent containing citric acid, succinic acid, tannic acid, salicylic acid, malic acid, ascorbic acid, gallic acid, hydrochloric acid, nitric acid, and / or acetic acid. The alkaline aqueous base is an aqueous solvent that includes sodium bicarbonate, sodium carbonate, sodium hydroxide, potassium bicarbonate, potassium carbonate, and / or potassium hydroxide. The protective material 200 is sprayed or applied to the surface of the plant organ to which the coating material 100 has been applied, and the stably polymerized glue coats the functional material on the surface of the plant organ to form a protective layer, thereby increasing the amount and density of the functional material that enters the stomata or leaf veins.

[0044] In one embodiment, the coated body 100 and the protected body 200, which are the plant surface modified bodies of the present invention, are manufactured as follows, taking as examples liquid or gel-like, or even layer-like, coated body 100 and protected body 200. To produce the coated body 100, first, a functional material suspension solvent containing the functional material 10 is mixed with an aqueous solvent using bioglue or biodegradable glue to form an adhesive functional material suspension solvent. Here, the functional material suspension solvent 10 containing the functional material further contains a pore expander such as 0 to 170 μM or less of fusicoccin to promote the expansion of multiple stomata on the surface of the plant organ. Next, by mixing this sticky functional material suspension solvent with an acidic or alkaline aqueous solvent, a sticky functional material suspension with an adjustable surface charge is formed, which is a functional material suspension solvent that is useful for applying to a plant organ 300 so as to cover the plant organ 300 with a coating 100 of the functional material 10. When preparing the protective body 200, a polymeric stabilizing material such as alum ions is mixed into an aqueous solvent using bioglue or biodegradable glue to form the protective body 200 used as described above to coat the surface of the coated body 100. The ion concentration is 0 to 20% by weight of the solvent. The ions polymerize and stabilize the gel.

[0045] Carbon sequestration tests were conducted on a common indoor plant, dieffenbachia (average 235g), and after a 15-day light cycle (1 / 10 of the 8 hours of sunlight exposure per day), the luminescent dieffenbachia as a normal dieffenbachia was found to be able to sequester 856mg of carbon, while the luminescent dieffenbachia that was 50% applied to the leaves and absorbed through the leaf veins was found to be able to sequester 1204mg of carbon, which means that carbon sequestration capacity can be increased by 40.6%.Luminescent plants can create a new experience for ornamental plants at night, and can simultaneously capture and neutralize carbon, improving carbon sequestration capacity by 40.6%.

[0046] As described above, the plant vein absorption method and functional material for plant modification of the present invention are a completely new technology for functional modification of plant surfaces, which can effectively impart special physical or chemical functions by modifying the plant surface. Moreover, even after modification, the plant can still grow naturally, and can be widely and effectively applied to natural plants, effectively promoting the progress of horticulture and agriculture through functional plants. Furthermore, by using plant surface modification technology, functional materials that can maintain and even coexist with the plant surface can remain functionally on the plant surface until the plant organs naturally undergo apoptosis. Plant surface modification technology allows special functions to be achieved without modifying or introducing plant genes.

[0047] The foregoing description is by way of example only and is not intended to be limiting. Any equivalent modifications or variations thereto that do not depart from the spirit and scope of the present invention are intended to be encompassed within the scope of the claims. [Explanation of symbols]

[0048] 1. Modified plant surface 10 Functional materials 20 Polymerization stabilizing materials 100 Covering 200 Protective Body 300 plant organs 302 Surface

Claims

1. A plant leaf vein absorption method, comprising: Hydrogen peroxide (HP) 6.5 g to 16.5 g, polyvinyl pyrrolidone (PVP) 2 g to 30 g, polyvinyl alcohol (PVA) 3 g to 9 g, nystatin 1,000 Units / mL to 900,000 Units / mL, and sulfuric acid (H ２ SO ４ subjecting the plant organs to a pretreatment step using a mixture of 550 μL of the soluble cellulose extract (96%) mixed with 50 g to 500 g of deionized water; A step of forming a viscous functional material suspension solvent by mixing a functional material suspension solvent containing a functional material that is a material that can cause the plant to emit light at a color temperature of an adjustable emission spectrum with an aqueous solvent using bioglue or biodegradable glue; mixing the adhesive functional material suspension solvent with an acidic or alkaline aqueous solvent to form an adhesive functional material suspension having an adjustable surface charge as a coating having a functional material; a step of covering a surface of the plant organ with the covering body, wherein the functional material suspension medium containing the functional material further contains a pore expander for promoting expansion of a plurality of pores on the surface of the plant organ so that the functional material is absorbed into the plant through a plurality of leaf veins of the plant organ, the content of the pore expander in the covering body is 170 μM, the zeta potential due to the surface charge of the functional material is +75 meV, and the plant organ is a leaf or a transformed leaf; forming a protective body by mixing a polymeric stabilizing material with another aqueous solvent using a bioglue or biodegradable glue, wherein the weight percentage of the polymeric stabilizing material in the protective body is 35% and the weight percentage of the bioglue or biodegradable glue in the protective body is 63%; and covering the surface of the covering body with the protective body, wherein the polymerized stabilizing material can stabilize and polymerize the bioglue or biodegradable glue of the protective body and / or the covering body, thereby increasing the absorption amount and density of the functional material by the plant organ and increasing the carbon sequestration ability of the plant. Plant leaf vein absorption method.

2. The plant vein absorption method described in claim 1, wherein when the plant is Dieffenbachia, the carbon sequestration ability of the plant increases by 40.6% after a 15-day light test (1 / 10 sunlight exposure of 8 hours per day).

3. 2. The plant leaf vein absorption method according to claim 1, wherein the bioglue of the protective body and / or the covering body is soft deer glue, hard deer glue, swimbladder glue, cow glue, rabbit glue, seedlac and / or Sansenbon glue, and the biodegradable glue is polyamide, polyvinyl pyrrolidone and / or polyvinyl acetate.

4. 2. The plant vein absorption method of claim 1, wherein the pore expander is fusicoccin and the polymeric stabilizing material is alum ions.

5. The plant leaf vein absorption method according to claim 1 , wherein the covering material is sprayed, applied or coated on the plant organ, and the protection material is sprayed, applied or coated on the covering material.

6. The plant leaf vein absorption method according to claim 1, wherein the particle size of the functional material is 2 μm to 5 nm.

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