Plasma fractions as treatments for age-related cognitive impairment

Abstract

To provide methods and compositions for treating and / or preventing aging-related conditions.SOLUTION: Provided is a method of treating a cognitive disorder, comprising administering an effective amount of a plasma fraction to a subject diagnosed with a cognitive disorder. Preferably, the plasma fraction is a plasma protein fraction.SELECTED DRAWING: Figure 5

Description

[Technical Field]

[0001] The present invention relates to the prevention and treatment of age-related diseases. The present invention relates to the use of blood products, such as plasma fractions, to treat and / or prevent age-related conditions, such as neurocognitive and neurodegenerative disorders. [Background technology]

[0002] The following is provided as background information and is not admitted to be prior art to the present invention.

[0003] Aging is an important risk factor for multiple human diseases, including cognitive impairment, cancer, arthritis, vision loss, osteoporosis, diabetes, cardiovascular disease, and stroke. In addition to the normal synapse loss during natural aging, synapse loss is an early pathological event common to many neurodegenerative conditions and is the primary correlate of the neural and cognitive dysfunction associated with these conditions. Therefore, aging remains the single most dominant risk factor for dementia-related neurodegenerative diseases such as Alzheimer's disease (AD) (Bishop, NA et al., Neural mechanisms of aging and cognitive decline. Nature 464(7288), 529-535(2010); Heeden, T. et al., Insights into the aging mind: a view from cognitive neuroscience. Nat. Rev. Neurosci. 5(2), 87-96(2004); Mattson, MP, et al., Aging and neuronal vulnerability. Nat. Rev. Neurosci. 7(4), 278-294(2006)).

[0004] Aging affects all tissues and functions of the body, including the central nervous system, and declines in functions such as cognition can have a serious impact on quality of life. Treatments for cognitive decline and neurodegenerative disorders have had limited success in preventing and reversing functional impairment. Therefore, it is important to identify new treatments to prevent, counter, or reverse the effects of aging, thereby maintaining cognitive integrity. Summary of the Invention

[0005] The present invention is based on the generation and use of blood products for the treatment and / or prevention of age-related disorders, such as cognitive impairment conditions, age-related dementia, and neurodegenerative diseases. The present invention recognizes, inter alia, the need for new therapies for the treatment and / or prevention of cognitive impairment, age-related dementia, and neurodegenerative diseases. The present blood and plasma-derived compositions of the present invention address the failures and shortcomings of current therapies by utilizing plasma fractions that exhibit efficacy in the treatment and / or prevention of cognitive impairment, age-related dementia, and neurodegenerative diseases. Furthermore, the present invention relates to proteins identified in the plasma fractions that may themselves be effective as therapeutic or prophylactic agents for cognitive impairment and age-related dementia, or that serve as targets for blockade by additional agents.

[0006] The present invention also recognizes that differences in protein content between different plasma fractions (e.g., fractions, effluents, "plasma fractions," plasma protein fractions, human albumin solutions) may contribute to the prevention and / or amelioration of certain cognitive impairments and alleviation of neurodegenerative diseases. By way of example and not limitation, embodiments of the present invention demonstrate that simply the high albumin concentration of Human Albumin Solution (HAS) preparations is not the driving force behind the cognitive improvements associated with Plasma Protein Fraction (PPF) preparations with lower albumin concentrations.

[0007] Blood and plasma from young donors have been shown to improve and reverse pre-existing effects of brain aging, including at the molecular, structural, functional, and cognitive levels (Saul A. Villeda, et al. Young blood reverses age-related impairments in cognitive function and synaptic plasticity in mice. Nature Medicine 20 659-663 (2014)). The present invention relates to plasma fractions and effluents, some of which have previously been used to treat shock in patients. The present invention also relates to the discovery that these are effective methods for treating age-related cognitive dysfunction.

[0008] Thus, according to an embodiment of the present invention, there is provided a method for treating age-related cognitive impairment, age-related dementia, and / or neurodegenerative disease using a blood product fraction of plasma. An embodiment of the method comprises administering the plasma fraction to an individual suffering from or at risk of developing age-related cognitive impairment or a neurodegenerative disease. A further embodiment of the method comprises administering a plasma fraction derived from a donor pool within a specific age range to an individual suffering from or at risk of developing age-related cognitive impairment. Reagents, devices, and kits useful for practicing the method are also provided.

[0009] In one embodiment, the plasma fraction can be one of several plasma fractions obtained from a blood fractionation process, such as the Cohn fractionation process described below. In another embodiment, the plasma fraction can be of the type referred to herein as a "plasma fraction," which is a solution containing normal human albumin, alpha and beta globulins, gamma globulins, and other proteins, either individually or in complexes. In another embodiment, the plasma fraction can be of the type known to those skilled in the art as a "plasma protein fraction" (PPF). In another embodiment, the plasma fraction can be a "human albumin solution" (HAS) fraction. In yet another embodiment, the plasma fraction can have substantially all of the clotting factors removed to preserve the efficacy of the fraction with a reduced risk of thrombosis. Embodiments of the present invention can also include administering fractions derived from, for example, a young donor or a young donor pool. Another embodiment of the present invention can include monitoring cognitive improvement in subjects treated with the plasma fraction.

[0010] All publications and patent applications mentioned in this specification are herein incorporated by reference to the same extent as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference.

[0011] The accompanying drawings illustrate embodiments of the invention and, together with this description, serve to explain the invention. These drawings are offered by way of example and not by way of limitation. It is emphasized that various features of the drawings may not be to scale. [Brief explanation of the drawings]

[0012] [Figure 1] Rearing latency of 3- or 13-month-old NSG mice treated with control, PPF1, or HAS1 is shown when placed in an open field chamber for 15 minutes. [Figure 2] Shown is the locomotor speed of 3- or 13-month-old NSG mice treated with control, PPF1, or HAS1 placed in an open field chamber for 15 minutes. [Figure 3] Shown is the distance traveled by 3- or 13-month-old NSG mice treated with control, PPF1, or HAS1 when placed in an open field chamber for 15 minutes. [Figure 4] 1 shows the time spent in the novel arm in a cued Y-maze test by 3- or 13-month-old NSG mice treated with control, PPF1, or HAS1. [Figure 5] Figure 1 shows the ratio of time spent in the novel arm to the familiar arm (novel:familiar ratio) in 3- or 13-month-old NSG mice treated with control, PPF1, or HAS1 in a cued Y-maze test. [Figure 6] 1 shows the movement speed in a cued Y-maze test of 3- or 13-month-old NSG mice treated with control, PPF1, or HAS1. [Figure 7] 1 shows the distance traveled in a cued Y-maze test by 3- or 13-month-old NSG mice treated with control, PPF1, or HAS1. [Figure 8] (A) Percentage of freezing time in a contextual fear conditioning test of memory for 3- and 13-month-old NSG mice treated with control, PPF1, or HAS1. (B) Percentage of freezing time in an auditory cued fear conditioning test of memory for 3- and 13-month-old NSG mice treated with control, PPF1, or HAS1. [Figure 9] Quantification of the percent freezing time during the last 90 seconds of a cued fear conditioning test for memory in 3- and 13-month-old NSG mice treated with control, PPF1, or HAS1. [Figure 10] (A) Chart of Barnes maze latency, testing spatial memory. The latency for 3- and 13-month-old NSG mice treated with control, PPF1, or HAS1 to reach the target hole is shown. (B) Quantification of the average of the last three trials shown in (A). [Figure 11]A, quantification of the number of cells staining positive for doublecortin (Dcx), a marker of newborn neurons, in the dentate gyrus of 3- and 13-month-old NSG mice treated twice weekly with control, PPF1, or HAS1 for up to 6 months. B, quantification of the number of cells staining positive for Ki67, a marker of proliferating cells, in the dentate gyrus of 3- and 13-month-old NSG mice treated twice weekly with control, PPF1, or HAS1 for up to 6 months. [Figure 12] The number of cells staining positive for Dcx was quantified in 13-month-old NSG mice treated with control, PPF1, 1x concentrated HAS1, or 5x concentrated HAS1 three times a week for 5 weeks. [Figure 13] The number of cells staining positive for Ki67 was quantified in 13-month-old NSG mice treated with control, PPF1, 1x concentrated HAS1, or 5x concentrated HAS1 three times a week for 5 weeks. [Figure 14] (A) shows the number of rearings in the open field chamber of NODscid mice treated intravenously with either saline (control) or PPF1 via tail vein injection twice a week starting at 6 months of age. Rearing was measured over a 15-minute span after placing the mice in the open field chamber. (B) shows the locomotor speed in the open field chamber of mice treated intravenously with either saline (control) or PPF1 via tail vein injection twice a week starting at 6 months of age. Speed ​​was measured over a 15-minute span after placing the mice in the open field chamber. (C) shows the distance traveled in the open field chamber of mice treated intravenously with either saline (control) or PPF1 via tail vein injection twice a week starting at 6 months of age. Speed ​​was measured over a 15-minute span after placing the mice in the open field chamber. [Figure 15]Barnes maze latency and hippocampus-dependent spatial learning and memory are shown. The latency to reach the target hole is shown for aged NSG mice (12 months old) treated with 150 μL of saline control, young plasma, effluent I, or effluent II / III. [Figure 16] Figure 1 shows the effects of young human plasma, PPF1, and saline control on hippocampus-dependent spatial learning and memory in male aged NSG mice (12 months old). Mice were treated with 150 μL of purified young human plasma (young plasma), PPF1, or saline three times per week (iv) for four weeks, and twice per week during weeks 5 and 6, the weeks in which the reported tests were performed. The latency to reach the hole in the Barnes maze is shown for each treatment group. [Figure 17] Figure 1 shows the effects of young human plasma, PPF1, and saline control on the mean latency to find the target hole in the Barnes maze during the last three trials of each test day. Aged NSG mice (12 months old) were treated with 150 μL of purified young human plasma (young plasma), PPF1, or saline three times (iv) per week for 4 weeks, followed by treatment twice per week during weeks 5 and 6, the weeks in which testing was performed. [Figure 18] The effects of young human plasma, PPF1, and saline control on cell viability as determined by BrdU detection are shown. Aged NSG mice (12 months old) were treated with 150 μL of purified young human plasma (young plasma), PPF1, or saline three times per week (iv) for 4 weeks, followed by twice per week during weeks 5 and 6, the weeks when behavioral testing was performed. Hippocampal slices were analyzed after sacrifice. [Figure 19] 1 shows the effect of control, PPF1, and HAS1 on the proliferation of neurospheres in cortical cultures. Shown are example images of neurospheres in cortical cultures after 21 days of in vitro culture, imaged with Tuj1, DAPI, or Tuj1 and DAPI. [Figure 20] 1 shows the effects of control, PPF1, and HAS1 on net neurite length in cortical cultures. [Figure 21]The effects of vehicle, PPF1, and HAS1 on sphere and process growth in cortical cultures are shown. Yellow shading highlights spheres and pink shading highlights neurites as determined by the IncuCyte software algorithm (Essen BioScience, Inc., Ann Arbor, MI). [Figure 22] A shows the number of neurospheres quantified as a percentage of vehicle in the cortex of E14-15 mouse embryos suspended in Neurobasal Medium supplemented with B27 and 2 mM Glutamax (vehicle), PPF1 (10% of a 5% stock solution), or HAS1 (10% of a 5% stock solution).B shows the neurite length quantified as a percentage of vehicle in the cortex of E14-15 mouse embryos suspended in Neurobasal Medium supplemented with B27 and 2 mM Glutamax (vehicle), PPF1 (10% of a 5% stock solution), or HAS1 (10% of a 5% stock solution). (C) Neurite branch points were quantified as a percentage of vehicle in the cortex of E14-15 mouse embryos suspended in Neurobasal medium supplemented with B27 and 2 mM Glutamax (vehicle), PPF1 (10% of a 5% stock solution), or HAS1 (10% of a 5% stock solution). (D) Neurosphere size was quantified as a percentage of vehicle in the cortex of E14-15 mouse embryos suspended in Neurobasal medium supplemented with B27 and 2 mM Glutamax (vehicle), PPF1 (10% of a 5% stock solution), or HAS1 (10% of a 5% stock solution). [Figure 23] Quantification of the number of neurospheres staining positive for Sox2 treated with control vehicle (Neurobasal medium supplemented with B27 and 2 mM Glutamax), PPF1 (10% of a 5% stock solution), or HAS1 (10% of a 5% stock solution) is shown. Sox2 staining is an indicator of the neurogenic potential of the neurospheres. DETAILED DESCRIPTION OF THE INVENTION

[0013] 1. Introduction The present invention relates to the identification and discovery of methods and compositions for the treatment and / or prevention of cognitive impairment, including age-related dementia and neurodegenerative diseases. Described herein are methods and compositions for treating subjects suffering from such disorders, aspects of the invention. The methods and compositions described herein are useful in preventing cognitive impairment, age-related dementia, and neurodegenerative diseases, ameliorating the symptoms of cognitive impairment, age-related dementia, and neurodegenerative diseases, slowing the progression of age-related cognitive impairment, age-related dementia, and neurodegenerative diseases, and / or reversing the progression of age-related cognitive impairment, age-related dementia, and neurodegenerative diseases. One embodiment of the present invention involves the use of a plasma fraction, such as one or more fractions or effluent obtained from a blood fractionation process, such as the Cohn fractionation process described below, as a treatment. One embodiment of the present invention involves the use of a plasma fraction (a solution containing normal human albumin, alpha and beta globulins, gamma globulins, and other proteins, individually or in complexes, hereinafter referred to as a "plasma fraction"). Another embodiment of the invention includes using a plasma protein fraction (PPF) as a treatment. Another embodiment of the invention includes using a human albumin solution (HAS) fraction as a treatment. Yet another embodiment includes using an effluent from a blood fractionation process, such as Effluent I or Effluent II / III, described below. A further embodiment includes a plasma fraction from which substantially all clotting factors have been removed to reduce the risk of thrombosis while retaining efficacy (see, e.g., U.S. Patent Application No. 62 / 236,710, incorporated herein by reference in its entirety).

[0014] Before describing the present invention in detail, it is to be understood that this invention is not limited to the particular methods or compositions described, as these may, of course, vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to be limiting, since the scope of the present invention will be limited only by the appended claims.

[0015] The publications discussed herein are provided solely for their disclosure prior to the filing date of the present application. Nothing herein should be construed as an admission that the present invention is not entitled to antedate such publication by virtue of prior invention. Further, the dates of publication provided may be different from the actual publication dates, which may need to be independently confirmed.

[0016] Where a range of values ​​is provided, each intervening value between the upper and lower limit of that range is also understood to be specifically disclosed to the tenth of the unit of the lower limit, unless the context clearly indicates otherwise. Each smaller range between any stated or intervening value in a stated range and any other stated or intervening value in that stated range is encompassed within the invention. The upper and lower limits of such smaller ranges may independently be included or excluded from the range, and each range where either or both limits are included in such smaller ranges, or where neither limit is included in such smaller ranges, is also encompassed within the invention, unless there is a specifically excluded limit within the stated range. When a stated range includes one or both of the limits, ranges excluding either or both of those included limits are also included in the invention.

[0017] However, the claims may be drafted to exclude any optional element, and therefore, this statement is intended to serve as a basis precedent for using exclusive terminology such as "solely," "only," and the like, or for using a "negative" limitation in connection with the recitation of claim elements.

[0018] As will be apparent to those skilled in the art upon reading this disclosure, each of the individual embodiments described and illustrated herein has individual components and features that may be readily separated from or combined with the features of any of the other several embodiments without departing from the scope and spirit of the invention. Any recited method may be carried out in the order of events recited or in any other order that is logically possible.

[0019] 2.Definition Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, some potentially preferred methods and materials are now described. All publications mentioned herein are incorporated by reference to disclose and describe the methods and / or materials in connection with the cited publications. In case of conflict, it is understood that the present disclosure supersedes any disclosure content of the incorporated publication.

[0020] It should be noted that, as used in this specification and the appended claims, the singular forms "a," "an," and "the" include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to "a cell" includes a plural of such cells, reference to "the peptide" includes reference to one or more peptides and equivalents thereof known to those skilled in the art, e.g., polypeptides, and so forth.

[0021] In describing the methods of the present invention, the terms "host," "subject," "individual," and "patient" are used interchangeably and refer to any mammal in need of such treatment according to the methods of the present disclosure. Such mammals include, for example, humans, sheep, cows, horses, pigs, dogs, cats, non-human primates, mice, and rats. In certain embodiments, the subject is a non-human mammal. In some embodiments, the subject is a livestock animal. In other embodiments, the subject is a pet. In some embodiments, the subject is a mammal. In certain instances, the subject is a human. Other subjects can include domestic pets (e.g., dogs and cats), livestock (e.g., cows, pigs, goats, horses, etc.), rodents (e.g., mice, guinea pigs, and rats, e.g., in animal models of disease), and non-human primates (e.g., chimpanzees and monkeys). Thus, subjects of the present invention include, but are not limited to, mammals, such as humans and other primates, such as chimpanzees and other apes and monkey species; in certain embodiments, the subject is a human. The term subject is also intended to include an individual or organism of any age, weight, or other physical characteristic, and the subject may be an adult, child, infant, or newborn.

[0022] "Young" or "young individual" refers to an individual who is 40 years of age or younger, e.g., 35 years of age or younger, e.g., 30 years of age or younger, e.g., 25 years of age or younger, or 22 years of age or younger. In some cases, the individual from whom the blood product, including young plasma, is derived is 10 years of age or younger, e.g., 5 years of age or younger, e.g., 1 year of age or younger. In some cases, when a plasma product is collected from the umbilical cord of a newborn, the subject is a newborn and the source of the plasma product is the umbilical cord. Thus, "young" and "young individual" can refer to a subject aged 0-40 years, e.g., 0, 1, 5, 10, 15, 20, 25, 30, 35, or 40 years. In other cases, "young" and "young individual" can refer to biological age (as opposed to chronological age), such as an individual who does not exhibit levels of inflammatory cytokines in plasma exhibited in older individuals. Conversely, these "young" and "young individual" may refer to biological age (as opposed to chronological age), such as an individual exhibiting a high level of anti-inflammatory cytokines in plasma compared to the levels in relatively older individuals. By way of example and not limitation, the inflammatory cytokine is eotaxin, and the fold difference between young subjects or young individuals and older individuals is at least 20%. Similarly, the fold difference of other inflammatory cytokines between older and younger individuals may be used to refer to biological age (see U.S. Patent Application No. 13 / 575,437, incorporated herein by reference). Typically, the individual is healthy. For example, the individual does not have a hematological malignancy or autoimmune disease at the time of collection.

[0023] "An individual suffering from or at risk of suffering from age-related cognitive impairment" refers to an individual who has passed more than about 50% of their life expectancy, for example, more than 60%, for example, more than 70%, for example, more than 75%, 80%, 85%, 90%, 95%, or even more than 99% of their life expectancy. The age of the individual depends on the species of interest. Thus, this percentage is based on the expected life expectancy of the species of interest. For example, in humans, such individuals may be 50 years or older, e.g., 60 years or older, 70 years or older, 80 years or older, 90 years or older, and usually up to 100 years of age, e.g., 90 years of age, i.e., between about 50 and 100 years of age, e.g., 50...55...60...65...70...75...80...85...90...95...100 years or older, or any age between 50 and 1000, suffering from an age-related condition, e.g., cognitive impairment, as described further below, associated with the natural aging process. and individuals of any age who have not yet developed cognitive impairment due to an age-related disease, such as individuals of any age who have not yet developed cognitive impairment, but who have not yet begun to show symptoms of cognitive impairment, such as individuals of about 50 years or older, e.g., 60 years or older, 70 years or older, 80 years or older, 90 years or older, and usually up to 100 years of age, i.e., between about 50 and 100 years of age, e.g., 50...55...60...65...70...75...80...85...90...95...100 years of age, as well as individuals of any age who suffer from cognitive impairment due to an age-related disease, as described below, and individuals of any age who have been diagnosed with an age-related disease generally associated with cognitive impairment, but who have not yet begun to show symptoms of cognitive impairment. Corresponding ages for non-human subjects are known and are intended to apply herein.

[0024] As used herein, "treatment" refers to either (i) the prevention of a disease or disorder, or (ii) the reduction or elimination of symptoms of a disease or disorder. Treatment may be effected prophylactically (before the onset of the disease) or therapeutically (after the onset of the disease). The effect may be prophylactic, in the sense of completely or partially preventing the disease or its symptoms, and / or therapeutic, in the sense of partially or completely curing the disease and / or adverse effects that may result from the disease. Thus, the term "treatment" as used herein covers any treatment of age-related diseases or disorders in mammals and includes (a) preventing the onset of the disease in a subject who may be predisposed to the disease but has not yet been diagnosed as having it, (b) arresting the disease, i.e., halting its development, or (c) relieving the disease, i.e., causing regression of the disease. Treatment may result in a variety of different physical manifestations, such as modulation of gene expression, tissue or organ regeneration, etc. Therapeutic agents may be administered before, during, or after the onset of the disease. Of particular interest is the treatment of ongoing disease, where the treatment stabilizes or reduces the patient's undesired clinical symptoms.Such treatment can be performed before the function of the affected tissue is completely lost.The therapy can be administered during the symptomatic stage of the disease, or in some cases, after the symptomatic stage of the disease.

[0025] In some embodiments, the age-related condition to be treated is age-related dysfunction in an individual's cognitive abilities. Cognitive abilities, or "cognition," refers to mental processes, including attention and concentration, learning complex tasks and concepts, memory (acquiring, retaining, and recalling new information in the short and / or long term), information processing (handling information gathered by the five senses), visuospatial function (visual perception, depth perception, use of mental imagery, copying drawings, constructing objects or shapes), language production and comprehension, verbal fluency (word finding), problem solving, decision-making, and executive function (planning and prioritization). "Cognitive decline" refers to the progressive decline of one or more of these abilities, such as a decline in memory, language, thinking, judgment, etc. "Cognitive impairment" and "cognitive impairment" refer to a decrease in cognitive ability compared to a healthy individual, e.g., an age-matched healthy individual, or compared to the individual's performance at a previous time, e.g., 2 weeks, 1 month, 2 months, 3 months, 6 months, 1 year, 2 years, 5 years, or 10 years or more ago. "Age-related cognitive impairment" refers to cognitive impairments commonly associated with aging, including, for example, cognitive impairments associated with the natural aging process, e.g., mild cognitive impairment (MCI), and cognitive impairments associated with age-related disorders, i.e., disorders that occur with increasing frequency as the aging process progresses, e.g., neurodegenerative conditions such as Alzheimer's disease, Parkinson's disease, frontotemporal dementia, Huntington's disease, amyotrophic lateral sclerosis, multiple sclerosis, glaucoma, myotonic dystrophy, and vascular dementia.

[0026] Blood products containing plasma components. In practicing the present methods, blood products containing plasma components are administered to an individual in need thereof, e.g., an individual suffering from or at risk of suffering from cognitive impairment and / or age-related dementia. Thus, methods according to embodiments of the present invention involve administering a blood product containing plasma components from an individual (a "donor individual" or "donor") to an individual (a "recipient individual" or "recipient") who is at least at risk of suffering from or suffering from cognitive impairment and / or age-related dementia. A "blood product containing plasma components" refers to any blood-derived product (e.g., whole blood, plasma, or a fraction thereof) that contains plasma. The term "plasma" is used in its conventional sense to refer to the straw-colored / pale yellow liquid component of blood, which is composed of approximately 92% water, 7% proteins, e.g., albumin, gamma globulin, antihemophilic factor, and other clotting factors, and 1% inorganic salts, sugars, fats, hormones, and vitamins. Non-limiting examples of plasma-containing blood products suitable for use in the present methods include whole blood treated with anticoagulants (e.g., EDTA, citrate, oxalate, heparin, etc.), blood products produced by filtering whole blood to remove white blood cells ("leukoreduction"), blood products consisting of plasmapheretically-derived or apheretically-derived plasma, fresh frozen plasma, blood products consisting essentially of purified plasma, and blood products consisting essentially of plasma fractions. In some cases, the plasma product used is a non-whole blood plasma product, meaning that the product is not whole blood and therefore lacks one or more components found in whole blood, e.g., red blood cells, white blood cells, etc., at least to the extent that these components are present in whole blood. In some cases, the plasma product is substantially, if not completely, acellular, in which case the cell content may be 5% or less by volume, e.g., 1% or less by volume, e.g., 0.5% or less by volume, and in some cases, acellular plasma fractions are compositions that are completely devoid of cells, i.e., they are cell-free.

[0027] Collection of blood products including plasma components. Embodiments of the methods described herein include administration of blood products including plasma components, which may be derived from a donor, such as a human volunteer. The term "human-derived" may refer to such products. Methods for collecting blood products, including plasma, from a donor are well known in the art (see, e.g., AABB TECHNICAL MANUAL, (Mark A. Fung, et al., eds., 18th ed. 2014), which is incorporated herein by reference).

[0028] In one embodiment, donations are obtained by venipuncture. In another embodiment, venipuncture is a single venipuncture. In another embodiment, saline hydration is not used. In one embodiment, the process of plasmapheresis is used to obtain blood products, including plasma. Plasmapheresis can involve removing a volume of plasma and returning the cellular components to the donor. In this embodiment, sodium citrate is used during plasmapheresis to prevent cellular coagulation. The volume of plasma collected from the donor is preferably 690-880 mL after citrate administration, and is preferably in line with the donor's body weight.

[0029] 3. Plasma Fraction During World War II, a need arose for a stable plasma expander that could be used when soldiers lost large amounts of blood on the battlefield. As a result, methods for preparing freeze-dried plasma were developed. However, the need for sterile water for reconstitution made the use of freeze-dried plasma difficult in combat situations. Dr. E. J. Cohn suggested that albumin could be used as an alternative and prepared a ready-to-use, stable solution that could be immediately administered for the treatment of shock (see Johan Vandersande, Current Approaches to the Preparation of Plasma Fractions in (Biotechnology of Blood) 165 (Jack Goldstein ed., 1st ed. 1991)). Dr. Cohn's plasma fraction purification procedure utilizes cold ethanol for its denaturing effect and uses changes in pH and temperature to achieve separation.

[0030] One embodiment of the methods described herein involves administering a plasma fraction to a subject. Fractionation is a process in which a specific subset of proteins is separated from plasma. Fractionation techniques are known in the art and are based on steps developed by Cohn et al. in the 1940s (E. Cohn, Preparation and properties of serum and plasma proteins. IV.A system for the separation into fractions of the protein and lipoprotein components of biological tissues and fluids. 68 J Am Chem Soc 459 (1946)). This process involves several steps, each of which uses a specific ethanol concentration, as well as changes in pH, temperature, and osmolality, to selectively precipitate proteins. The precipitates are then separated by centrifugation or sedimentation. The original "Cohn fractionation process" involved separating proteins by precipitation into five fractions designated Fraction I, Fraction II+III, Fraction IV-1, Fraction IV-4, and Fraction V. Albumin was the first identified endpoint (fraction V) product of this process.According to embodiments of the present invention, each fraction (or the effluent from a preceding separation step) contains or may contain a therapeutically useful protein fraction (see Thierry Burnouf, Modern Plasma Fractionation, 21(2) Transfusion Medicine Reviews 101 (2007); Adil Denizli, Plasma fractionation: conventional and chromatographic methods for albumin purification, 4 J. Biol. & Chem. 315, (2011); and T. Brodniewicz-Proba, Human Plasma Fractionation and the Impact of New Technologies on the Use and Quality of Plasma-derived Products, 5 Blood Reviews 245 (1991); and U.S. Pat. Nos. 3,869,431, 5,110,907, 5,219,995, 7,531,513, and 8,772,461, which are incorporated herein by reference). The above experimental parameters may be adjusted to obtain a particular protein fraction.

[0031] In more recent years, fractionation has reached greater complexity and thus constitutes a further embodiment of the present invention. This recent increase in complexity has occurred through the introduction of chromatography techniques resulting in the isolation of new proteins from existing fractions such as cryoprecipitate, cryo-poor plasma, and Cohn fractions, increased IgG recovery through the integration of chromatography and ethanol fractionation processes, and viral reduction / inactivation / removal (Id.). Anion exchange chromatography can be used to capture proteins at physiological pH and ionic strength, which preserves the functional activity of the protein and / or protein fraction. Heparin and monoclonal antibodies have also been used in affinity chromatography. Those skilled in the art will recognize that the above parameters can be adjusted to obtain fractions containing specifically desired plasma proteins.

[0032] In one embodiment of the present invention, plasma is fractionated in an industrial setting. Frozen plasma is thawed at 1°C to 4°C. Continuous refrigerated centrifugation is applied to the thawed plasma to isolate the cryoprecipitate. The collected cryoprecipitate is frozen at or below -30°C and stored. The cryoprecipitate-poor ("cryopoor") plasma is immediately processed (e.g., by primary chromatography) for capture of labile coagulation factors, such as factor IX complex and its components, as well as protease inhibitors, such as antithrombin and C1 esterase inhibitor. Subsequent steps of continuous centrifugation and isolation of the precipitate can be applied. Such techniques are known to those skilled in the art and are described, for example, in U.S. Pat. Nos. 4,624,780, 5,219,995, and 5,288,853, and U.S. Patent Application Nos. 20140343255 and 20150343025, the disclosures of which are incorporated herein by reference in their entireties.

[0033] In one embodiment of the present invention, the plasma fraction may include a plasma fraction containing a significant concentration of albumin. In another embodiment of the present invention, the plasma fraction may include a plasma fraction containing a significant concentration of IgG or intravenous immunoglobulin (IGIV) (e.g., Gamunex-C®). In another embodiment of the present invention, the plasma fraction may include an IGIV plasma fraction, such as Gamunex-C®, that has been substantially depleted of immunoglobulin (IgG) by methods well known to those skilled in the art, such as protein A-mediated depletion (see Keshishian, H., et al., Multiplexed, Quantitative Workflow for Sensitive Biomarker Discovery in Plasma Yields Novel Candidates for Early Myocardial Injury, Molecular & Cellular Proteomics, 14 at 2375-93 (2015)). In a further embodiment, the plasma fraction may be one from which substantially all coagulation factors have been removed, such that the efficacy of the fraction is maintained with a reduced risk of thrombosis. For example, the plasma fraction may be the plasma fraction described in U.S. Patent No. 62 / 376,529, filed August 18, 2016, the disclosure of which is incorporated herein by reference in its entirety.

[0034] 4. Albumin products Those skilled in the art will recognize that albumin plasma products ("APP") are broadly classified into two categories: plasma protein fraction (PPF) and human albumin solution (HAS). PPF is derived from a process that produces a higher yield than HAS but has a lower albumin purity (>83% for PPF and >95% for HAS) than HAS (Production of human albumin solution: a continually developing colloid, P. Matejtschuk et al., British Journal of Anaesthesia 85(6):887-95, at 888 (2000)). Furthermore, some have pointed out that PPF has disadvantages due to the presence of protein "contaminants" such as PKA (ibid.). As a result, PPF preparations have fallen out of favor as albumin plasma products and have even been removed from the pharmacopoeias of certain countries (ibid.). Contrary to these concerns, the present invention effectively utilizes these "contaminants." In addition to the aforementioned PKA, as well as alpha, beta, and gamma globulins, the methods of the present invention utilize additional proteins or other factors in the "contaminant" that promote processes such as neurogenesis, neuronal survival, and cognitive improvement.

[0035] Those skilled in the art will recognize that there are or have been several commercial sources of PPF ("commercial PPF preparations"), including Plasma-Plex™ PPF (Armour Pharmaceutical Co., Tarrytown, NY), Plasmanate™ PPF (Grifols, Clayton, NC), Plasmatein™ (Alpha Therapeutics, Los Angeles, CA), and Protenate™ PPF (Baxter Labs, Inc., Deerfield, IL).

[0036] Those skilled in the art will also recognize that there are or have been several commercial sources of HAS ("commercial HAS preparations"), including Albuminar™ (CSL Behring), AlbuRx™ (CSL Behring), Albutein™ (Grifols, Clayton, NC), Buminate™ (Baxatla, Inc., Bannockburn, IL), Flexbumin™ (Baxatla, Inc., Bannockburn, IL), and Plasbumin™ (Grifols, Clayton, NC).

[0037] A. Plasma Protein Fraction (Human) (PPF) According to the United States Food and Drug Administration ("FDA"), "Plasma Protein Fraction (Human)," or PPF, is the official name for a product defined as "a sterile solution of proteins composed of albumins and globulins derived from human plasma" (Code of Federal Regulations "CFR" 21 CFR 640.90, which is incorporated herein by reference). The source of PPF is plasma recovered from whole blood prepared as specified in 21 CFR 640.1-640.5, which are incorporated herein by reference, or source plasma prepared as specified in 21 CFR 640.60-640.76, which are incorporated herein by reference.

[0038] PPF is tested in accordance with 21 CFR 640.92 (incorporated herein by reference) to determine that it meets the following criteria: (a) the final product is a 5.0±0.30 percent solution of protein; and (b) The total protein in the final product shall consist of at least 83 percent albumin and not more than 17 percent globulins. Not more than 1 percent of the total protein shall be gamma globulins. Protein composition shall be determined by methods approved for each manufacturer by the Director of the Center for Biologics Evaluation and Research of the Food and Drug Administration.

[0039] As used herein, "plasma protein fraction" or "PPF" refers to a sterile solution of proteins composed of albumin and globulins derived from human plasma, having an albumin content of at least 83%, globulins (including α1 globulin, α2 globulin, β globulin, and γ globulin) and other plasma proteins of not more than 17%, and gamma globulins of not more than 1% as determined by electrophoresis (Hink, JH, Jr., et al., Preparation and Properties of a Heat-Treated Human Plasma Protein Fraction, Vox Sanguinis 2(174)(1957)). PPF may also refer to a solid form having a similar composition when suspended in a solvent. The total globulin fraction can be determined by subtracting albumin from total protein (Busher, J., Serum Albumin and Globulin, CLINICAL METHODS: THE HISTORY, PHYSICAL, AND LABORATORY EXAMINATIONS, Chapter 10, Walker HK, Hall WD, Hurst JD, eds. (1990)).

[0040] B. Albumin (Human) (HAS) According to the FDA, "Albumin (Human)" (also referred to herein as "HAS") is the official name of a product defined as a "sterile solution of albumin derived from human plasma" (Code of Federal Regulations "CFR" 21 CFR 640.80, which is incorporated herein by reference). The source of Albumin (Human) is plasma recovered from whole blood prepared as specified in 21 CFR 640.1-640.5, which are incorporated herein by reference, or source plasma prepared as specified in 21 CFR 640.60-640.76, which are incorporated herein by reference. Other requirements for Albumin (Human) are listed in 21 CFR 640.80-640.84, which are incorporated herein by reference.

[0041] Albumin (human) is tested per 21 CFR 640.82 to determine if it meets the following criteria: (a) Protein Concentration. The final product shall correspond to one of the following concentrations: 4.0±0.25 percent, 5.0±0.30 percent, 20.0±1.2 percent, and 25.0±1.5 percent protein solutions. (b) Protein Composition. At least 96 percent of the total protein in the finished product must be albumin, as determined by a method approved for each manufacturer by the Director of the Center for Biologics Evaluation and Research of the Food and Drug Administration.

[0042] As used herein, "albumin (human)" or "HAS" refers to a sterile solution of proteins composed of albumin and globulins derived from human plasma, with at least 95% albumin content and no more than 5% globulins (including α1 globulin, α2 globulin, β globulin, and γ globulin) and other plasma proteins. HAS may also refer to a solid form with a similar composition when suspended in a solvent. The total globulin fraction may be determined by subtracting albumin from the total protein.

[0043] As will be appreciated by those skilled in the art, the PPF and HAS fractions may be in lyophilized or other solid forms. Such preparations can be used with appropriate additives to form, for example, tablets, powders, granules, or capsules. Solid forms can be formulated into injectable preparations by dissolving, suspending, or emulsifying them in aqueous or non-aqueous solvents, such as vegetable oils or other similar oils, synthetic fatty acid glycerides, esters of higher fatty acids, or propylene glycol, and, if desired, with conventional additives such as solubilizers, isotonicity agents, suspending agents, emulsifiers, stabilizers, and preservatives.

[0044] 5. Fractions with reduced coagulation factors Another embodiment of the present invention uses plasma fractions in which substantially all of the coagulation factors have been removed, such that the efficacy of the fraction is maintained with a reduced risk of thrombosis. Conveniently, blood products may be derived from a young donor or young donor pool and can be devoid of IgM to provide an ABO-compatible young blood product. Currently, transfused plasma is matched to ABO blood types because the presence of naturally occurring antibodies against A and B antigens can result in transfusion reactions. IgM is believed to be a contributing factor to transfusion reactions when ABO-incompatible plasma is given to patients. Removal of IgM from blood products or fractions helps avoid transfusion reactions in subjects receiving the blood products and plasma fractions of the present invention.

[0045] Thus, in one embodiment, the present invention relates to a method of treating or preventing an age-related condition, such as cognitive impairment or neurodegeneration, in a subject. The method comprises administering to the subject a blood product or blood fraction derived from whole blood of an individual or pool of individuals, wherein the blood product or blood fraction substantially lacks (a) at least one clotting factor and / or (b) IgM. In some embodiments, the individual(s) from whom the blood product or blood fraction is derived are young individuals. In some embodiments, the blood product substantially lacks at least one clotting factor and IgM. In certain embodiments, the blood product substantially lacks fibrinogen (Factor I). In further embodiments, the blood product substantially lacks red blood cells and / or white blood cells. In further embodiments, the blood product is substantially acellular. In other embodiments, the blood product is derived from plasma. Such embodiments of the present invention are further supported by U.S. Patent Application No. 62 / 376,529, filed August 18, 2016, the entire contents of which are incorporated herein by reference.

[0046] 6. Protein-enriched plasma protein products Further embodiments of the invention utilize plasma fractions that have reduced albumin concentrations but increased amounts of globulins and other plasma proteins (some termed "contaminants") compared to PPF. These embodiments, like PPF, HAS, Effluent I, and Effluent II / III, are all effectively devoid of coagulation factors. Such plasma fractions are hereafter referred to as "protein-enriched plasma protein products." For example, one embodiment of the invention may utilize a protein-enriched plasma protein product consisting of 82% albumin and 18% α-, β-, and γ-globulins and other plasma proteins. Another embodiment of the invention may utilize a protein-enriched plasma protein product consisting of 81% albumin and 19% α-, β-, and γ-globulins and / or other plasma proteins. Another embodiment of the invention may utilize a protein-enriched plasma protein product consisting of 80% albumin and 20% α-, β-, and γ-globulins and / or other plasma proteins. Further embodiments of the invention may use a protein-enriched plasma protein product consisting of 70-79% albumin and a corresponding 21-30% of α-, β-, and γ-globulins and other plasma proteins. Further embodiments of the invention may use a protein-enriched plasma protein product consisting of 60-69% albumin and a corresponding 31-40% of α-, β-, and γ-globulins and other plasma proteins. Further embodiments of the invention may use a protein-enriched plasma protein product consisting of 50-59% albumin and a corresponding 41-50% of α-, β-, and γ-globulins and other plasma proteins. Further embodiments of the invention may use a protein-enriched plasma protein product consisting of 40-49% albumin and a corresponding 51-60% of α-, β-, and γ-globulins and other plasma proteins. Further embodiments of the invention may use a protein-enriched plasma protein product consisting of 30-39% albumin and a corresponding 61-70% of α-, β-, and γ-globulins and other plasma proteins. A further embodiment of the present invention may use a protein-enriched plasma protein product consisting of 20-29% albumin and a corresponding 71-80% of alpha, beta, and gamma globulins and other plasma proteins.Further embodiments of the invention may use a protein-enriched plasma protein product consisting of 10-19% albumin and a corresponding 81-90% of alpha, beta, and gamma globulins and other plasma proteins. Further embodiments of the invention may use a protein-enriched plasma protein product consisting of 1-9% albumin and a corresponding 91-99% of alpha, beta, and gamma globulins and other plasma proteins. Further embodiments of the invention may use a protein-enriched plasma protein product consisting of 0-1% albumin and 99-100% of alpha, beta, and gamma globulins and other plasma proteins.

[0047] The above-described embodiments of the invention may have a total gamma globulin concentration of 0-5%.

[0048] The specific concentration of a protein in a plasma fraction can be determined using techniques well known to those skilled in the relevant art, including, by way of example and not limitation, electrophoresis, mass spectrometry, ELISA analysis, and Western blot analysis.

[0049] 7. Preparation of Plasma Fractions Methods for preparing PPF and other plasma fractions are well known to those skilled in the art. According to one embodiment of the present invention, blood used to prepare human plasma protein fractions can be collected in flasks containing citrate or anticoagulant citrate dextrose solution to prevent coagulation, and fractions I, II+III, IV, and PPF can be further separated according to the method disclosed by Hink et al. (See Hink, JH, Jr., et al., Preparation and Properties of a Heat-Treated Human Plasma Protein Fraction, VOX SANGUINIS 2(174)(1957), incorporated herein by reference). According to this method, the mixture can be collected at 2-8°C. The plasma can then be separated by centrifugation at 7°C, removed, and stored at -20°C. The plasma can then be thawed at 37°C and fractionated, preferably within 8 hours of removal from -20°C storage.

[0050] Plasma can be separated from Fraction I using 8% ethanol at a protein concentration of 5.1 to 5.6 percent, pH 7.2, and a temperature of -2 to -2.5°C. Cold 53.3% ethanol (176 mL / L of plasma) can be added along with acetate buffer (200 mL of 4 M sodium acetate, 230 mL of glacial acetic acid, and H2O up to 1 L) using a jet at a rate of, for example, 450 mL / min, while the plasma temperature is lowered to -2°C. Fraction I can be separated and isolated from the effluent (Effluent I) by ultracentrifugation. Fibrinogen can be obtained from Fraction I according to methods well known to those skilled in the art.

[0051] Fraction II+III can be separated from Effluent I by adjusting the effluent to 21 percent ethanol at a protein concentration of 4.3 percent and a temperature of -6°C at pH 6.8. While lowering the temperature of Effluent I to -6°C, 95 percent cold ethanol (176 mL / L of Effluent I) can be added, for example, using a jet at a rate of 500 mL / min, along with 10 M acetic acid used for pH adjustment. The resulting precipitate (Fraction II+III) can be removed by centrifugation at -6°C. Gamma globulin can be obtained from Fraction II+III using methods well known to those skilled in the art.

[0052] Fraction IV-1 can be isolated from Effluent II+III ("Effluent II / III") by adjusting the effluent to 19% ethanol at a protein concentration of 3% and a pH of 5.2 at -6°C. While Effluent II / III is maintained at -6°C for 6 hours, HO and 10 M acetic acid, used for pH adjustment, can be added using a jet. The precipitated Fraction VI-1 can be allowed to settle at -6°C for 6 hours and then separated from the effluent by centrifugation at the same temperature. At a protein concentration of 2.5% and a pH of 4.65, a temperature of -7°C, and a stable plasma protein fraction can be recovered from Effluent IV-1 by adjusting the ethanol concentration to 30%. This can be achieved by adjusting the pH of Effluent IV-1 with cold acid-alcohol (2 parts 2 M acetic acid and 1 part 95% ethanol). While maintaining the temperature at -7°C, 170 mL of cold ethanol (95%) is added for every liter of adjusted Effluent IV-1. Precipitating proteins can be removed by centrifugation at -7°C after settling for 36 hours.

[0053] The recovered protein (stable plasma protein fraction) can be dried (e.g., by lyophilization) to remove alcohol and HO. The resulting dried powder can be dissolved in sterile distilled water, e.g., using 15 liters of water per kg of powder, and the solution adjusted to pH 7.0 with 1 M NaOH. A final protein concentration of 5 percent can be achieved by adding sterile distilled water containing sodium acetyltryptophan, sodium caprylate, and NaCl to a final concentration of 0.004 M acetyltryptophan salt, 0.004 M caprylate, and 0.112 M sodium. Finally, the solution can be filtered at 10°C to obtain a clear solution, followed by heat treatment at 60°C for at least 10 hours to inactivate pathogens.

[0054] Those skilled in the art will recognize that each of the various fractions and effluents described above can be used in conjunction with the methods of the present invention to treat diseases. For example, and not by way of limitation, Effluent I or Effluent II / III can be utilized to treat diseases such as cognitive disorders and neurodegenerative disorders, and are embodiments of the present invention.

[0055] The above-described methods for preparing plasma fractions and plasma protein fractions (PPF) are merely exemplary and relate to embodiments of the present invention. Those skilled in the art will recognize that these methods can be modified. For example, in various embodiments and methods of the present invention, various variations of plasma fractions and plasma protein fractions can be produced by adjusting, among other things, pH, temperature, and ethanol concentration. In another example, a further embodiment of the present invention contemplates the use of nanofiltration for the removal / inactivation of pathogens in plasma fractions and plasma protein fractions.

[0056] Further embodiments of the present invention contemplate methods and compositions that use and / or include additional plasma fractions. For example, the present invention demonstrates, inter alia, that a specific concentration of albumin is not essential to improve cognitive activity. Thus, fractions with reduced albumin concentrations, e.g., fractions with less than 83% albumin, are contemplated by the present invention.

[0057] 8. Treatment Aspects of the methods of the invention described herein include treating a subject with a plasma-containing blood product, e.g., a plasma fraction, e.g., as described above. One embodiment includes treating a human subject with a plasma-containing blood product. Those skilled in the art will recognize that methods of treating a subject with plasma-containing blood products are known in the art. By way of example and not limitation, one embodiment of the methods of the invention described herein includes administering fresh frozen plasma to a subject for the treatment and / or prevention of cognitive impairment and / or age-related dementia. In one embodiment, the plasma-containing blood product is administered immediately, e.g., within about 12-48 hours after collection from a donor, to an individual suffering from or at risk of cognitive impairment and / or age-related dementia. In such cases, the product may be stored under refrigeration, e.g., at 0-10°C. In another embodiment, the fresh frozen plasma has been frozen (cryopreserved) at -18°C or below. The fresh frozen plasma is thawed prior to administration and administered to the subject immediately, e.g., 60-75 minutes after the thawing process begins. Each subject preferably receives a single unit of fresh frozen plasma (200-250 mL). This fresh frozen plasma is preferably derived from donors within a predetermined age range. In one embodiment of the present invention, the fresh frozen plasma is donated from (derived from) a younger individual. In another embodiment of the present invention, the fresh frozen plasma is donated from (derived from) a donor of the same gender. In another embodiment of the present invention, the fresh frozen plasma is donated from (derived from) a donor within the age range of 18-22 years. In one embodiment, subjects are treated twice weekly with 3-4 days between infusions. In one embodiment of the present invention, treatment is continued until a specific endpoint is reached.

[0058] In one embodiment of the present invention, blood products, including plasma, are screened by blood type after donation. In another embodiment of the present invention, blood products, including plasma, are screened for infectious agents, such as HIV I&II, HBV, HCV, HTLV I&II, and anti-HBc, in accordance with the requirements of 21 CFR 640.33 and recommendations contained in FDA guidance documents.

[0059] In yet another embodiment of the present invention, the subject is treated with a "plasma fraction." In one embodiment of the present invention, the plasma fraction is PPF or HAS. In a further embodiment of the present invention, the plasma fraction is one of a commercial PPF preparation or a commercial HAS preparation. In another embodiment of the present invention, the plasma fraction is PPF or HAS derived from a pool of individuals of a particular age range, such as young individuals, or is a modified PPF or HAS fraction (e.g., PPF or HAS from which one or more specific proteins have been partially or substantially removed) that has been subjected to further fractionation or processing. In another embodiment of the present invention, the plasma fraction is an IGIV plasma fraction that is substantially depleted of immunoglobulins (IgG). A blood fraction that is "substantially depleted" or "substantially removed" of a particular protein, such as IgG, refers to a blood fraction that contains less than about 50%, e.g., less than 45%, 40%, 35%, 30%, 25%, 20%, 15%, 5%, 4%, 3%, 2%, 1%, 0.5%, 0.25%, 0.1%, an undetectable level, or any integer between these values, of the amount occurring in a reference product or whole blood plasma, as measured using standard assays well known in the art.

[0060] 9. Monitoring Another aspect of the present invention relates to a method for monitoring the effects of a drug on a subject for the treatment of cognitive impairment and / or age-related dementia, comprising comparing cognitive function before and after treatment. Those skilled in the art will recognize that there are well-known methods for assessing cognitive function. For example, but not by way of limitation, the method may include assessment of cognitive function based on a medical history, family history, physical and neurological examinations by a clinician specializing in dementia and cognitive function, laboratory tests, and neuropsychological evaluation. Further embodiments contemplated by the present invention include assessment of state of consciousness using, for example, the Glasgow Coma Scale (EMV), mental status tests including the Abbreviated Mental Test Score (AMTS) or the Mini-Mental State Examination (MMSE) (Folstein et al., J. Psychiatr. Res 1975;12:1289-198), a global assessment of higher-order functions, and estimation of intracranial pressure by funduscopy, etc.

[0061] In one embodiment, peripheral nervous system testing can be used to assess cognitive function, including smell, visual field and vision, eye movements and pupils (sympathetic and parasympathetic), facial sensory function, strength of the muscles of the face and shoulder girdle, hearing, taste, pharyngeal movements and reflexes, tongue movements (which can be tested individually (e.g., visual acuity can be tested with a Snellen eye chart, and reflexes including the masseter, biceps, and triceps tendons, hamstrings, Achilles tendon reflex, and plantar reflex (i.e., Babinski sign) are tested with the use of a reflex hammer)), muscle strength, muscle tone, and signs of rigidity, frequently on an MRC scale of 1-5.

[0062] 10. Administration In practicing the methods of the present invention, a plasma fraction is administered to a subject. In one embodiment, the plasma fraction is administered by intravenous infusion. The rate of infusion can vary, but in one embodiment of the present invention, the infusion rate is 5-8 mL / min. Those skilled in the art will recognize that the infusion rate can depend on the subject's condition and response to administration.

[0063] In embodiments in which an effective amount of an active agent is administered to an adult mammal, the amount or dosage is effective if it is administered for a suitable period, such as one week or more, for example, two weeks or more, for example, three weeks or more, one month or more, two months or more, three months or more, four months or more, five months or more, six months or more, one year or more, etc., and results in a reduction in the condition of the adult mammal, such as a delay in cognitive impairment and / or cognitive improvement. For example, an effective dose is one that, when administered over a suitable period, slows cognitive decline by, for example, about 20% or more, e.g., 30% or more, 40% or more, or 50% or more, and in some cases, 60% or more, 70% or more, 80% or more, or 90% or more. For example, it halts cognitive decline in patients suffering from natural aging or age-related disorders. In some cases, an effective amount or dose of a blood product not only slows or halts the progression of a disease state, but also induces a reversal of the condition, i.e., results in an improvement in cognitive ability. For example, in some cases, an effective amount is an amount that, when administered for a suitable period, usually at least about 1 week, possibly about 2 weeks or more, depending on the individual, about 3 weeks, 4 weeks, 8 weeks or more, improves the cognitive performance of an individual suffering from age-related cognitive impairment, for example, 1.5 times, 2 times, 3 times, 4 times, 5 times, in some cases 6 times, 7 times, 8 times, 9 times, or 10 times or more, compared to the cognition before administration of the blood product or fraction.In some cases, an effective amount or effective dose of an active agent not only slows or stops the progression of the disease state, but also induces the reversal of the condition.That is, it brings about the improvement of cognitive function.For example, in some cases, an effective amount is an amount that, when administered for a suitable period, improves the symptoms of an individual suffering from cognitive decline or impairment, for example, 1.5 times, 2 times, 3 times, 4 times, 5 times, in some cases 6 times, 7 times, 8 times, 9 times, or 10 times or more, compared to the untreated individual before administration of the drug.

[0064] In other embodiments, the plasma fraction or plasma fraction is administered according to one or more dosing regimens described in U.S. Patent Application No. 62 / 490,519, the entirety of which is incorporated herein by reference. Accordingly, one embodiment of the present invention involves treating a subject diagnosed with cognitive impairment by administering to the subject an effective amount of plasma or plasma fraction, wherein the plasma or plasma fraction is administered in a manner that results in improved cognitive function or neurogenesis after the mean or median half-life of the plasma or plasma fraction protein is reached compared to the most recent dose (referred to herein as "pulse dosing" or "pulse-dosed"). Another embodiment of the present invention involves administering plasma or plasma fraction according to a dosing regimen of at least two consecutive days and monitoring the subject for improved cognitive function at least three days after the date of the last administration. Further embodiments of the invention include administering plasma or a plasma fraction according to a dosing regimen of at least 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, or 14 consecutive days and monitoring the subject for improvement in cognitive function at least 3 days after the last administration. Yet another embodiment of the invention includes administering plasma or a plasma fraction according to a dosing regimen of at least 2 consecutive days and monitoring for improvement in cognition after the last administration, once the average half-life of proteins in the plasma or plasma fraction has been reached. Another embodiment of the invention includes administering plasma or a plasma fraction according to a dosing regimen of 2 to 14 consecutive days, where each interval between doses may be 0 to 3 days. In some cases, pulse dosing according to the invention includes administration of a first set of doses, e.g., as described above, followed by a period of no dosing, e.g., a "dose-free period," followed by administration of another dose or set of doses. The length of this "medication-free" period can vary, but in some embodiments is 7 days or more, e.g., 10 days or more, e.g., 14 days or more, and in some cases the drug-free period ranges from 15 to 365 days, e.g., 30 to 90 days, e.g., 30 to 60 days. Thus, embodiments of the present methods include non-chronic (i.e., non-sustained) medication, e.g., non-chronic administration of plasma products.In some embodiments, the pattern of pulse dosing followed by a drug-free period is repeated as many times as desired, and in some cases, the pattern continues for one year or more, e.g., two years or more, up to and including the lifetime of the subject. Another embodiment of the invention involves administering plasma or a plasma fraction according to a five-day dosing regimen, which includes a two- to three-day drug-free period followed by two to fourteen consecutive days of dosing.

[0065] Biochemically, an "effective amount" or "effective dose" of an active agent refers to an amount of active agent that prevents, antagonizes, reduces, decreases, or inhibits cognitive impairment or age-related dementia by about 20% or more, e.g., 30% or more, 40% or more, or 50% or more, and in some cases 60% or more, 70% or more, 80% or more, or 90% or more, and in some cases about 100%, i.e., to a negligible extent, and in some cases reverses its progression.

[0066] 11. Plasma Protein Fractions In practicing the methods of the present invention, a plasma fraction is administered to a subject. In one embodiment, the plasma fraction is a plasma protein fraction (PPF). In a further embodiment, the PPF is selected from a commercial PPF preparation.

[0067] In another embodiment, the PPF contains 88% normal human albumin, 12% alpha and beta globulins, and 1% or less gamma globulins, as determined by electrophoresis. Embodiments of this embodiment used in the practice of the methods of the invention include, for example, a 5% solution of PPF buffered with sodium carbonate and stabilized with 0.004 M sodium caprylate and 0.004 M acetyltryptophan. Additional formulations, such as those that modify the concentration of solvents and stabilizers as well as the percentage of PPF in solution (e.g., from about 1% to about 10%, from about 10% to about 20%, from about 20% to 25%, from about 25% to 30%), may also be utilized in the practice of the methods of the invention.

[0068] 12. Plasma fractions of specific donor ages One embodiment of the present invention involves administering plasma fractions or plasma fractions derived from the plasma of individuals within a particular age range. A further embodiment involves administering plasma protein fractions derived from the plasma of individuals within a particular age range. One embodiment involves administering PPF or HAS derived from the plasma of young individuals. In another embodiment of the invention, the young individuals are of a single particular age or a particular age range. In yet another embodiment, the average age of the donors is less than the age of the subject or less than the average age of the subjects being treated.

[0069] Certain embodiments of the present invention involve pooling blood or plasma from individuals of a particular age range and fractionating the plasma as described above to obtain a plasma protein fraction product, such as PPF or HAS. In alternative embodiments of the present invention, the plasma protein fraction or a particular plasma protein fraction is obtained from a particular individual falling within a specified age range. In another embodiment of the present invention, the plasma fraction, plasma fraction, or a particular plasma protein fraction product is obtained from a pool of younger individuals, where "young" may be determined by chronological age or biological age as described above, and the age(s) of the individuals may be of a particular age or age range.

[0070] 13. Indications The methods and blood products and fractions, including plasma, are useful for treating, e.g., preventing, age-related conditions, e.g., impairment of an individual's cognitive abilities, e.g., cognitive impairment, including, but not limited to, age-related dementia, immunological conditions, cancer, and physical and functional decline. Individuals suffering from or at risk of developing age-related cognitive impairment and who may benefit from treatment with blood products, including plasma, of the present invention, e.g., according to the methods disclosed herein, include individuals aged about 50 years or older, e.g., 60 years or older, 70 years or older, 80 years or older, 90 years or older, and 100 years or older, i.e., between about 50 and 100 years old, e.g., 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, or about 100 years old, Cognitive impairments associated with the natural aging process include individuals suffering from mild cognitive impairment (MCI), and individuals about 50 years of age or older, e.g., 60 years of age or older, 70 years of age or older, 80 years of age or older, 90 years of age or older, and typically under 100 years of age, i.e., about 50-90 years of age, e.g., 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, or about 100 years of age, who have not yet begun to show symptoms of cognitive impairment. Examples of cognitive impairments due to natural aging include:

[0071] A. Mild Cognitive Impairment. Mild cognitive impairment (MCI) is a moderate disruption of cognition that manifests as problems with memory or other mental functions, such as planning, following instructions, or decision-making, over time, while overall mental function and everyday activities remain intact. Thus, although significant neuronal death does not generally occur, neurons in the aging brain are susceptible to sublethal age-related changes in structure, synaptic integrity, and molecular processing at synapses, all of which impair cognitive function.

[0072] Individuals suffering from or at risk of developing age-related cognitive impairment and who may benefit from treatment with the blood products or fractions, including plasma, of the present invention, e.g., according to the methods disclosed herein, include individuals of any age who suffer from cognitive impairment resulting from an age-related disorder, as well as individuals of any age who have been diagnosed with an age-related disorder commonly associated with cognitive impairment, but who have not yet begun to exhibit symptoms of cognitive impairment. Examples of such age-related disorders include:

[0073] B. Alzheimer's Disease. Alzheimer's disease is a progressive, unstoppable loss of cognitive function associated with excessive numbers of senile plaques in the cerebral cortex and subcortical gray matter, including neurofibrillary tangles composed of b-amyloid and tau proteins. Common forms affect people over the age of 60, and its incidence increases with age. Alzheimer's disease accounts for over 65% of dementia in older adults.

[0074] The cause of Alzheimer's disease is unknown. The disease is familial in approximately 15-20% of cases. The remaining so-called sporadic cases have some genetic determinant. The disease has an autosomal dominant genetic pattern in most early-onset cases and some late-onset cases, with variable penetrance in later life. Environmental factors are the focus of active investigation.

[0075] During the course of the disease, synaptic and ultimately neuronal loss occurs in the cerebral cortex, hippocampus, and subcortical structures (including selective cell loss in the nucleus basalis of Meynert), the locus coeruleus, and the dorsal raphe nucleus. Cerebral glucose utilization and perfusion are reduced in some brain regions (parietal and temporal cortices in early disease, prefrontal cortex in late disease). The pathogenesis of Alzheimer's disease involves the formation of neuritic or senile plaques (composed of neurites, astrocytes, and glial cells around an amyloid core) and neurofibrillary tangles (composed of paired helical filaments). Senile plaques and neurofibrillary tangles occur with normal aging but are much more common in people with Alzheimer's disease.

[0076] C. Parkinson's Disease. Parkinson's disease (PD) is an idiopathic, slowly progressive, degenerative CNS disorder characterized by slowed movements, muscle rigidity, resting tremor, and postural instability. Originally considered primarily a movement disorder, PD is now recognized to also affect cognition, behavior, sleep, autonomic function, and sensory function. The most common cognitive impairments include impairments in attention and concentration, working memory, executive function, language production, and visuospatial function.

[0077] In primary Parkinson's disease, pigmented neurons in the substantia nigra, locus coeruleus, and other brainstem dopaminergic cell groups are lost. The cause is unknown. Loss of nigral neurons that project to the caudate nucleus and putamen leads to depletion of the neurotransmitter dopamine in these areas. Onset is generally after age 40, with an increased incidence in older populations.

[0078] Secondary parkinsonism results from the loss or disruption of dopamine action in the basal ganglia due to other idiopathic degenerative diseases, drugs, or exogenous toxins. The most common cause of secondary parkinsonism is the ingestion of antipsychotic drugs or reserpine, which block dopamine receptors, resulting in parkinsonism. Less common causes include carbon monoxide or manganese poisoning, hydrocephalus, structural lesions (tumors, infarctions affecting the midbrain or basal ganglia), subdural hematomas, and degenerative disorders including striatonigral degeneration.

[0079] D. Frontotemporal Dementia. Frontotemporal dementia (FTD) is a condition resulting from the progressive deterioration of the frontal lobes of the brain. Over time, degeneration may progress to the temporal lobes. Second in prevalence after Alzheimer's disease (AD), FTD accounts for 20% of presenile dementia cases. Symptoms are divided into three groups based on frontal and temporal lobe function.

[0080] Symptoms of behavioral FTD (bvFTD) include lethargy and loss of spontaneity on the one hand, and disinhibition on the other. In progressive non-fluent aphasia (PNFA), speech fluency is disrupted due to articulation difficulties and phonological and / or grammatical errors, but comprehension of language is preserved. In semantic dementia (SD), patients maintain fluency with normal phonology and grammar, but naming and comprehension of language become increasingly difficult. Other cognitive symptoms common to all FTD patients include impairments in executive function and concentration. Other cognitive abilities, including perception, spatial ability, memory, and executive functioning, generally remain intact. FTD can be diagnosed by observing atrophy of the frontal and / or anterior temporal lobes, as revealed by structural MRI scans.

[0081] FTD exists in several forms, all of which can be treated or prevented using the present methods and compositions. For example, one form of frontotemporal dementia is semantic dementia (SD). SD is characterized by loss of semantic memory in both verbal and nonverbal domains. Patients with SD often complain of word-finding difficulties. Clinical signs include fluent aphasia, anomia, impaired comprehension of word meanings, and associative visual agnosia (an inability to match semantically related pictures or objects). While cases of "pure" semantic dementia with few subsequent behavioral symptoms have been described, progression of the disease often results in behavioral and personality changes similar to those seen in frontotemporal dementia. Structural MRI imaging shows a characteristic pattern of temporal lobe atrophy (predominantly left), with inferior lesions greater than superior lesions and greater anterior temporal lobe atrophy than posterior temporal lobe atrophy.

[0082] As another example, another form of frontotemporal dementia is Pick's disease (PiD, also known as PcD). The defining feature of this disease is the accumulation of tau protein in neurons, forming silver-staining spherical aggregates known as "Pick bodies." Symptoms include loss of speech (aphasia) and dementia. Patients with orbitofrontal dysfunction can become aggressive and socially inept. They may steal or exhibit compulsive or repetitive stereotyped behaviors. Patients with dorsomedial or dorsolateral frontal dysfunction may exhibit apathy, blunted affect, or reduced spontaneity. Patients may exhibit a lack of self-monitoring, abnormal self-awareness, and an inability to comprehend meaning. Patients with gray matter loss in the bilateral posterolateral orbitofrontal cortex and right anterior insula may exhibit altered eating behavior, such as a pathological sweet preference. Patients with more focal gray matter loss in the anterior lateral orbitofrontal cortex may develop binge eating. Although some symptoms may initially be alleviated, the disease progresses and patients often die within 2 to 10 years.

[0083] E. Huntington's Disease. Huntington's disease (HD) is an inherited, progressive neurodegenerative disorder characterized by the development of emotional, behavioral, and psychiatric abnormalities, loss of intellectual or cognitive function, and movement abnormalities (dyspraxia). Typical signs of HD include the onset of chorea (involuntary, rapid, irregular, jerky movements that may affect the face, arms, legs, or trunk) and cognitive decline, including gradual loss of thought processing and acquired intellectual abilities. Impairments in memory, abstract thinking, and judgment, a loss of perception of time, place, or identity (disorientation), increased agitation, and personality changes (disorganization) may occur. Symptoms typically become apparent during the fourth or fifth decade, but the age at onset varies, ranging from early childhood to late adulthood (e.g., the seventh or eighth decade).

[0084] HD is transmitted in families as an autosomal dominant trait. The disorder results from an abnormally long sequence, or "repeat," of coded instructions in a gene on chromosome 4 (4p16.3). The progressive loss of nervous system function associated with HD results from the loss of neurons in certain regions of the brain, including the basal ganglia and cerebral cortex.

[0085] F. Amyotrophic Lateral Sclerosis. Amyotrophic lateral sclerosis (ALS) is a rapidly progressive and always fatal neurological disease that affects motor neurons. Muscle weakness and atrophy and signs of anterior horn cell dysfunction are most often initially present in the hands and less commonly in the feet. The site of onset is random, and progression is asymmetric. Muscle cramps are common and may precede weakness. Patients rarely survive for 30 years; 50% die within 3 years of onset, 20% survive for 5 years, and 10% survive for 10 years.

[0086] Diagnostic features include onset in mid- or late adulthood and progressive generalized movement disorders without sensory abnormalities. Nerve conduction velocities remain normal until late in the disease. Recent studies have also documented symptoms of cognitive dysfunction, particularly declines in immediate verbal memory, visual memory, language, and executive function.

[0087] In ALS patients, reductions in cell body area, synapse number, and total synaptic length have been reported, even in neurons that appear normal. It has been suggested that functional impairment may occur due to continued synapse loss once active zone plasticity reaches its limit. Promotion of new synapse formation or prevention of synapse loss may preserve neuronal function in these patients.

[0088] G. Multiple Sclerosis. Multiple sclerosis (MS) is characterized by a variety of symptoms and signs of CNS dysfunction, with relapsing remissions and exacerbations. The most common presenting symptoms are paresthesias in one or more limbs, the trunk, or one side of the face, weakness or ataxia of the legs or hands, or visual disturbances, such as partial blindness and pain in one eye (retrobulbar optic neuritis), dimness of vision, or a scotoma. Common cognitive dysfunctions include impairments in memory (acquiring, retaining, and recalling new information), attention and concentration (especially divided attention), information processing, executive function, visuospatial function, and verbal fluency. Common early symptoms are ophthalmoplegia resulting in double vision, transient weakness of one or more limbs, slight stiffness or unusual fatigability in the hands and feet, slight gait disturbance, difficulty with bladder control, dizziness, and mild emotional disturbance, all of which indicate diffuse CNS damage and often occur months or years before the disease is recognized. Excessive fever may accentuate symptoms and signs.

[0089] The course is highly variable and unpredictable and is remittent in most patients. Initially, there may be months or years of remission between episodes, especially if the disease begins with retrobulbar optic neuritis. However, some patients have frequent attacks and become rapidly incapacitating, and in a minority the course may be rapidly progressive.

[0090] H. Glaucoma. Glaucoma is a common neurodegenerative disease affecting retinal ganglion cells (RGCs). Evidence supports the existence of a compartmentalized degenerative program in synapses and dendrites containing RGCs. Recent evidence has also demonstrated a correlation between cognitive impairment and glaucoma in older adults (Yochim BP, et al. Prevalence of cognitive impairment, depression, and anxiety symptoms among older adults with glaucoma. J Glaucoma. 2012;21(4):250-254).

[0091] I. Myotonic Dystrophy. Myotonic dystrophy (DM) is an autosomal dominant, multisystem disorder characterized by dystrophic muscle weakness and myotonia. The molecular defect is a trinucleotide (CTG) repeat expansion in the 3' untranslated region of the myotonin protein kinase gene on chromosome 19q. Symptoms can occur at any age, and there is a wide range of clinical severity. Myotonia is prominent in the hand muscles, and ptosis is common even in mild cases. In severe cases, significant peripheral muscle weakness occurs and is often accompanied by cataracts, premature hair loss, axe-like facies, cardiac arrhythmias, testicular atrophy, and endocrine abnormalities (e.g., diabetes mellitus). Mental retardation is common in severe congenital forms, while milder adult forms of the disorder exhibit age-related decline in frontal and temporal cognitive function, particularly language and executive function. Severely affected individuals die by their early 50s.

[0092] J. Dementia. Dementia refers to a class of disorders in which symptoms affect thinking and social skills severely enough to interfere with daily functioning. Other instances of dementia include vascular dementia, discussed below, and dementia with Lewy bodies, in addition to the dementia observed in the later stages of age-related disorders discussed above.

[0093] In vascular dementia, or "multi-infarct dementia," cognitive impairment is caused by problems with the brain's blood supply, typically through a series of minor strokes, or occasionally through one major stroke preceded or followed by other, less severe strokes. Vascular lesions can be the result of diffuse cerebrovascular disease, such as small vessel disease, or focal lesions, or both. Patients with vascular dementia present with cognitive impairment acutely or subacutely after an acute cerebrovascular event, followed by progressive cognitive decline. Cognitive impairment is similar to that observed in Alzheimer's disease, including impairments in language, memory, complex visual processing, or executive function, but the associated brain changes are due to chronically reduced cerebral blood flow, which ultimately leads to dementia, rather than AD pathology. Neuroimaging with single-photon emission computed tomography (SPECT) and positron emission tomography (PET) can be used in conjunction with mental status evaluation to confirm a diagnosis of multi-infarct dementia.

[0094] Dementia with Lewy bodies (DLB, also known by various other names, including dementia with Lewy bodies, diffuse Lewy body disease, cortical Lewy body disease, and senile dementia of the Lewy type) is a type of dementia characterized anatomically by the presence of Lewy bodies (clusters of alpha-synuclein and ubiquitin proteins) in neurons, detectable on histological examination of postmortem brain. Its main feature is cognitive decline, particularly of executive function. Attention and short-term memory are variable.

[0095] Persistent or recurrent visual hallucinations with vivid and detailed descriptions are often early diagnostic symptoms. DLB is often confused with Alzheimer's disease and / or vascular dementia in its early stages, but whereas Alzheimer's disease has a fairly gradual onset, DLB often has a rapid or acute onset. DLB symptoms also include motor symptoms similar to those of Parkinson's disease. DLB is distinguished from the dementia that sometimes occurs in Parkinson's disease by the time frame in which dementia symptoms appear relative to Parkinson's disease symptoms. Parkinson's disease with dementia (POD) is diagnosed when the onset of dementia occurs more than one year after the onset of Parkinson's disease. DLB is diagnosed when cognitive symptoms begin simultaneously with or within one year of Parkinson's disease symptoms.

[0096] K. Progressive Supranuclear Palsy. Progressive supranuclear palsy (PSP) is a brain disorder that causes severe, progressive problems with gait and balance control, along with complex eye movements and thinking problems. One of the hallmarks of the disease is the inability to properly aim the eyes, due to lesions in the area of ​​the brain that coordinates eye movements. Some individuals describe this effect as blurred vision. Affected individuals often exhibit mood and behavior changes, including depression and apathy, as well as progressive mild dementia. The disorder's long name indicates that the disease begins slowly, continues to worsen (progressive), and causes weakness (paralysis) by damaging specific areas of the brain (supranuclear regions) above the pea-sized structures called nuclei that control eye movement. PSP was first described as a distinct disorder in 1964, when three scientists published a paper distinguishing the condition from Parkinson's disease. PSP is sometimes called Steele-Richardson-Olszewski syndrome, reflecting a combination of the names of the scientists who defined the disorder. PSP gradually worsens, but no one dies from it.

[0097] L. Ataxia. People with ataxia have problems with coordination because the parts of the nervous system that control movement and balance are affected. Ataxia can affect fingers, hands, arms, legs, body, speech, and eye movements. The term ataxia is often used to describe symptoms of incoordination that may be related to infection, injury, other disease, or degenerative changes in the central nervous system. Ataxia is also used to describe a group of specific degenerative disorders of the nervous system called hereditary and sporadic ataxias, which are the primary focus of the National Ataxia Foundation.

[0098] M. Multiple System Atrophy. Multiple system atrophy (MSA) is a neurodegenerative disorder. MSA is associated with the degeneration of nerve cells in specific areas of the brain. This cell degeneration causes problems with movement, balance, and other autonomic functions of the body, such as bladder control or blood pressure regulation.

[0099] The cause of MSA is unknown, and no specific risk factors have been identified. Approximately 55% of cases occur in men, with the typical age of onset being in the late 50s to early 60s. MSA often presents with some of the same symptoms as Parkinson's disease. However, patients with MSA typically respond poorly, if at all, to dopaminergic medications used for Parkinson's disease.

[0100] N. Frailty. Frailty syndrome ("frailty") is a geriatric syndrome characterized by functional and physical decline, including decreased mobility, muscle weakness, physical slowness, poor endurance, low physical activity, malnutrition, and involuntary weight loss. Such decline is often associated with cognitive dysfunction and the consequences of diseases such as cancer. However, frailty can occur in the absence of disease. Frail individuals are at increased risk for poor outcomes due to fractures, falls, disability, comorbidities, and premature death (C. Buigues, et al. Effect of a Prebiotic Formulation on Frailty Syndrome: A Randomized, Double-Blind Clinical Trial, Int. J. Mol. Sci. 2016, 17, 932). Furthermore, frail individuals are at increased risk for high medical costs (ibid.).

[0101] Common symptoms of frailty can be determined by certain types of tests. For example, unintentional weight loss involves a loss of at least 10 lbs or more than 5% of body weight in the previous year, muscle weakness can be determined by low grip strength in the bottom 20% (adjusted for gender and BMI) at baseline, physical slowness can be based on the time it takes to walk a 15-foot distance, lack of endurance can be determined by an individual's self-reported fatigue, and low physical activity can be measured using standardized questionnaires (Z. Palace et al., The Frailty Syndrome, Today's Geriatric Medicine 7(1), at 18(2014)).

[0102] In some embodiments, the methods and compositions are useful for slowing down the progression of age-related cognitive impairment. In other words, the cognitive ability of an individual declines more slowly after treatment with the methods of the present disclosure than before or without treatment with the methods of the present disclosure. In some such cases, the treatment method includes measuring the progression of cognitive decline after treatment and determining that the progression of cognitive decline is reduced. In some such cases, this determination is made by comparing with a reference subject, for example, the rate of cognitive decline in the individual before treatment, for example, determined by previously measuring cognition at two or more time points before administration of the blood product.

[0103] The present method and composition can also be used to stabilize the cognitive ability of individuals, such as individuals suffering from age-related cognitive decline or individuals at risk of suffering from age-related cognitive decline.For example, an individual may exhibit some age-related cognitive impairment, and the progression of cognitive impairment observed before treatment with the method of the present disclosure is stopped after treatment with the method of the present disclosure.As another example, an individual may be at risk of developing age-related cognitive decline (for example, the individual may be over 50 years old or may have been diagnosed with age-related disorders), and the cognitive ability of the individual is not substantially changed after treatment with the method of the present disclosure compared to before treatment with the method of the present disclosure.In other words, cognitive decline cannot be detected.

[0104] The present method and composition can also be used to reduce the cognitive impairment of individuals suffering from age-related cognitive impairment.In other words, the cognitive ability of individuals after treatment with the present method is improved.For example, the cognitive ability of individuals after treatment with the present method is improved by, for example, 2 times or more, 5 times or more, 10 times or more, 15 times or more, 20 times or more, 30 times or more, or 40 times or more, for example, 50 times or more, 60 times or more, 70 times or more, 80 times or more, 90 times or more, or 100 times or more, compared with the cognitive ability observed in individuals before treatment with the present method.In some cases, treatment with the present method and composition can restore the cognitive ability of individuals suffering from age-related cognitive decline to, for example, the level that the individuals had when they were about 40 years old or younger.In other words, cognitive impairment is suppressed.

[0105] 14. Methods for diagnosing and monitoring improvement in neurocognitive-related disorders In some cases, among various methods for diagnosing neurocognitive-related diseases and monitoring their progression and improvement, the following types of assessments are used alone or, if desired, in combination with an individual suffering from a neurodegenerative disease. The following types of methods are provided as examples and are not limited to the methods listed. If desired, any convenient method for monitoring disease may be used in the practice of the present invention. These methods are also contemplated by the methods of the present invention.

[0106] A. Global cognition Method embodiments of the present invention further include methods for monitoring the effects of a drug or treatment on a subject for the treatment of cognitive impairment and / or age-related dementia, comprising comparing cognitive function before and after treatment. Those skilled in the art will recognize that there are well-known methods for assessing cognitive function. For example, and without limitation, the methods may include assessment of cognitive function based on medical history, family history, physical and neurological examinations by clinicians specializing in dementia and cognitive function, laboratory tests, and neuropsychological evaluation. Further embodiments contemplated by the present invention include assessment of state of consciousness using, for example, the Glasgow Coma Scale (EMV), mental status tests including the Abbreviated Mental Test Score (AMTS) or the Mini-Mental State Examination (MMSE) (Folstein et al., J. Psychiatr. Res 1975;12:1289-198), global assessment of higher-order function, and estimation of intracranial pressure by funduscopy, etc.

[0107] In one embodiment, peripheral nervous system testing can be used to assess cognitive function, including smell, visual field and vision, eye movements and pupils (sympathetic and parasympathetic), facial sensory function, strength of the muscles of the face and shoulder girdle, hearing, taste, pharyngeal movements and reflexes, tongue movements (which can be tested individually (e.g., visual acuity can be tested with a Snellen eye chart, and reflexes including the masseter, biceps, and triceps tendons, hamstrings, Achilles tendon reflex, and plantar reflex (i.e., Babinski sign) are tested with the use of a reflex hammer)), muscle strength, muscle tone, and signs of rigidity, frequently on an MRC scale of 1-5.

[0108] 15. Reagents, Devices, and Kits Also provided are reagents, devices, and kits thereof for practicing one or more of the above methods. The reagents, devices, and kits thereof can vary widely.

[0109] Reagents and devices of interest include, for example, anticoagulants, cryopreservatives, buffers, isotonic solutions, etc., as described above with respect to the methods of preparing blood products, including plasma, for transfusion into a subject in need thereof.

[0110] The kit may also include blood collection bags, tubing, needles, centrifuge tubes, etc. In yet other embodiments, the kits described herein include two or more containers of plasma product, such as plasma protein fractions, e.g., three or more, four or more, five or more, e.g., six or more containers of plasma product. In some cases, the number of individual containers of plasma product in the kit may be nine or more, twelve or more, fifteen or more, eighteen or more, twenty-one or more, twenty-four or more, thirty or more, e.g., thirty-six or more, e.g., forty-eight or more. Each container may be associated with identifying information that includes various data regarding the plasma product contained therein, which may include one or more of the age of the donor of the plasma product, processing details regarding the plasma product, e.g., whether the plasma product has been processed to remove proteins above an average molecular weight (e.g., those described above), blood type details, etc. In some cases, each container in the kit includes identifying information for the plasma contained therein, including information regarding the donor age of the plasma product, e.g., the identifying information identifies data regarding the age of the plasma product donor (where such identifying information can be the donor's age at the time of collection). In some cases, each container in the kit contains plasma product derived from a donor of substantially the same age. That is, all of the containers contain product derived from donors of substantially, if not exactly the same, age. Substantially the same age means that the various donors from whom the plasma products in the kit are obtained differ from each other, in some cases, by 5 years or less, e.g., 4 years or less, e.g., 3 years or less, e.g., 2 years or less, e.g., 1 year or less, e.g., 9 months or less, 6 months or less, 3 months or less, e.g., 1 month or less. The identifying information may be present on any convenient container component, e.g., a label, RFID chip, etc. The identifying information may be human-readable, computer-readable, etc., as desired. The container may have any convenient configuration. The volume of the container can vary, but in some cases the volume ranges from 10 mL to 5000 mL, e.g., 25 mL to 2500 mL, e.g., 50 mL to 1000 mL, e.g., 100 mL to 500 mL. The container can be rigid or flexible and can be made from any convenient material, e.g., a polymeric material, including a medical-grade plastic material. In some cases, the container has a bag or pouch configuration.In addition to the container, such kits may further include an administration device, e.g., as described above. The components of such kits may be provided in any suitable packaging, e.g., a box or similar structure configured to contain the container and other kit components.

[0111] In addition to the above components, the kit further includes instructions for practicing the method. These instructions may be present in the kit in various forms, one or more of which may be present in the kit. One form in which these instructions may be present is information printed on a suitable medium or substrate, such as a sheet or sheets of paper on which the information is printed, in the kit's packaging, in a package insert, etc. Yet another means is a computer-readable medium on which the information is recorded, such as a diskette, CD, portable flash drive, etc. Yet another means may be a website address that can be used to access information on a remote site via the Internet. Any convenient means may be present in the kit.

[0112] 16. Experimental Procedure The following examples are put forth so as to provide those of ordinary skill in the art with a complete disclosure and description of how to make and use the present invention, and are not intended to represent that the experiments below represent all or the only experiments performed. Efforts have been made to ensure accuracy with respect to numbers used (e.g., amounts, temperatures, etc.), but some experimental error and deviation should be accounted for. Unless otherwise indicated, parts are parts by weight, molecular weight is weight average molecular weight, temperature is in degrees Celsius, and pressure is near atmospheric.

[0113] General methods in molecular and cellular biochemistry are described in Molecular Cloning: A Laboratory Manual, 3rd Ed. (Sambrook et al., Harvard Laboratory Press 2001), Short Protocols in Molecular Biology, 4th Ed. (Ausubel et al. eds., John Wiley & Sons 1999), Protein Methods (Bollag et al., John Wiley & Sons 1996), Nonviral Vectors for Gene Therapy (Wagner et al. eds., Academic Press 1999), Viral Vectors (Kaplift & Loewy eds., Academic Press 1995), Immunology Methods Manual (I. Lefkovits ed., Academic Press 1997), and Cell and Tissue Culture: Laboratory Precedures in Biotechnology (Doyle & Griffiths, John Wiley & Sons Genetic engineering techniques, such as those described in the "Genetic Engineering of Genes," can be found in standard textbooks, such as "Genetic Engineering of Genes," ...

[0114] A. Materials and Reagents USP saline was purchased from Hospira (Lake Forest, IL). Injections were performed using a 27.5G or 30G needle, with a volume of 150 μL per injection. Commercially available PPF ("PPF1"), such as the commercial PPF preparation described above in a 5% solution, was stored at 4° C. Commercially available HAS ("HAS1"), such as the commercial HAS preparation described above in a 5% solution, was stored at 4° C.

[0115] B. Animal Supply and Housing The mouse strains NOD.CB17-Prkdcscid / NcrCrl ("NODscid," strain code 394, Charles River, MA) (Bosma, M. et al., The scid mouse mutant. 137 Curr Top Microbiol Immunol 197 (1988)) and NODscid gamma ("NSG," strain code 005557, The Jackson Laboratory, Bar Harbor, ME) were used. Each mouse was ear-punched and assigned a unique identification number. All mice were individually housed under specific pathogen-free conditions with a 12-hour light / 12-hour dark cycle. All animal handling and use followed standard IACUC-approved guidelines.

[0116] C. Administration Unless otherwise stated below, NSG and NODscid mice were injected intravenously via tail vein injection (150 μL per injection) with USP saline, 5% PPF1, or 5% HAS1 twice a week for up to 6 months.

[0117] D. Open field The open field test was used to assess the exploratory behavior of subject mice. The open field test is an empty testing arena, usually circular or square. Mice are placed in a 50 cm x 50 cm open field arena for 15 minutes, and their activity level is measured. Rearing time was measured by tracking the duration the forelimbs remained in contact with the walls of the box. Total distance covered and speed were also measured throughout the test period. A CleverSys TopScan V3.0 (Reston, VA) was used to track the behavior of mice in the open field. The open field chamber was constructed by CleverSys.

[0118] EY Maze Mice were allowed to explore two arms (start + non-novel) of the Y-maze for 5 min. After 1 h, mice were allowed to explore all three arms and the total time and number of entries into these arms were recorded.

[0119] F.Barnes Maze Mice were trained for four consecutive days in a modified Barnes maze, with a maximum of 120 seconds to find the exit hole (Barnes, CA, Memory deficits associated with senescence: A neurophysiological and behavioral study in the rat, J. COMPARATIVE AND PHYSIOLOGICAL PSYCHOLOGY, 93(1):74-104 (1979); see also Faizi, M. et al., The Thy1-hAPP (Lond / Swe+) mouse model of Alzheimer's disease displays broad behavioral deficits in sensorimotor, cognitive, and social function., BRAIN BEHAV. 2(2):142-54 (2012) for a description of the modified maze). The exit hole remained the same for the four trials on a training day but was changed between training days. The latency to find the exit hole was recorded for each cohort of mice on each of the four training days.

[0120] G. DCX-positive cells and Ki67-positive cells Doublecortin (DCX) is a microtubule-associated protein expressed by neural progenitor cells and also by immature neurons within embryonic and adult cortical structures. Neural progenitor cells express DCX when they are actively dividing. The protein is downregulated after two weeks. Because of this association, it is useful as a marker of neurogenesis.

[0121] Brain tissue processing and immunohistochemistry were performed on free-floating sections using well-described techniques (Luo, J. et al. Glia-dependent TGF-β signaling, acting independently of the TH17 pathway, is critical for the initiation of murine autoimmune encephalomyelitis. J. CLIN. INVEST. 117, 3306-3315 (2007)). Mice were anesthetized and perfused with 0.9% saline. Brains were removed and fixed in 4% paraformaldehyde-phosphate buffer at pH 7.4 at 4°C, then submerged in 30% sucrose for cryoprotection. Brains were then sectioned at 30 μm using a cryomicrotome at -22°C. Sections were stored in cryoprotective medium. The primary antibodies used were goat anti-Dcx (Santa Cruz Biotechnology, 1:500 for twice-weekly dosing experiments or 1:200 for three-weekly dosing experiments) or rabbit anti-Ki67 (1:500, Abcam). Primary antibody staining was revealed using diaminobenzidine (DAB, Sigma-Aldrich) or fluorescently conjugated secondary antibodies, followed by biotinylated secondary antibodies and the ABCkit (Vector). To estimate the total number of Dcx-positive cells per dentate gyrus, immunopositive cells within the granule cell and subgranule cell layers of the dentate gyrus were counted and averaged in three coronal brain sections through the hippocampus.

[0122] H. Barnes maze testing of aged NSG mice treated with young plasma, effluent I, or effluent II / III. Aged NSG mice (12 months old) were divided into groups (n = 14 in total) and received 150 μL of saline, young plasma, effluent I, or effluent II / III via tail vein injection before the start of behavioral testing. Each separate group was divided into three cohorts, and behavioral testing began in different weeks for each cohort.

[0123] I. Barnes maze and cell viability (BrdU staining) of aged NSG mice treated with young plasma or PPF1 three times a week. Aged (12-month-old) male NSG mice were treated intravenously with 150 μL of purified young human plasma (young plasma), PPF1, or saline via tail vein injection three times per week for four weeks. This regimen was changed to twice per week during weeks 5 and 6, which were the behavioral testing weeks.

[0124] Prior to treatment, mice were divided into three cohorts of 13–15 mice each, and each cohort received intraperitoneal (ip) BrdU injections 5 days prior to the initiation of treatment with young plasma, PPF1, or saline.

[0125] During weeks 5 and 6, behavioral testing was conducted to measure the latency of each mouse to reach the target hole in a Barnes maze test. Each test session lasted a maximum of 120 seconds. Finding the target hole was recorded using software that determined when the mouse's nose entered the area defined as the target hole.

[0126] At the end of behavioral testing, animals were sacrificed and six sections per hippocampus were quantified using bright-field microscopy to determine the presence of BrdU-positive cells within the granule cell layer of the dentate gyrus. To obtain an estimate of the total number of BrdU-positive cells, the average number of BrdU-positive cells was multiplied by 72, the total number of sections per hippocampus for each animal, for representative sections across different regions of the hippocampus.

[0127] J. Neurosphere and Cortical Culture Assays 1. Tuj1 and DAPI staining Mouse C57 E14,15 cortices (Lonza:M-CX-300) were suspended in 12 mL of neurobasal medium supplemented with B27 and 2 mM Glutamax (Sigma-Aldrich). 200 μL was added to each well of a 96-well plate pre-coated with collagen I (Corning, Inc.). After 16 hours, the seeding medium was replaced with pre-warmed (37°C) control medium (neurobasal medium supplemented with B27 and 2 mM Glutamax (Gibco)). On day 4 of in vitro culture ("days in vitro" or "DIV"), the culture medium was replaced with fresh control medium, control medium and 10% PPF1, control medium and 10% HAS1, vehicle and 10% PPF1, or vehicle and 10% HAS1. Cultures were maintained for 21 days, with 75% of the medium replaced with fresh medium every 3 days. At 21 DIV, cultures were washed three times with PBS and then fixed with 4% paraformaldehyde for 20 minutes at room temperature (RT). After fixation, cultures were washed twice with PBS and then permeabilized with 0.1% Triton X100 for 5–20 minutes. After permeabilization, cultures were blocked with 3% bovine serum albumin (Sigma-Aldrich) for 60 minutes at RT. After 60 minutes, the blocking solution was aspirated, and cultures were labeled with anti-Tuj1 antibody (AbCam-1:500) overnight at 4°C. After labeling, cultures were washed three times with PBS + 0.1% BSA and then stained with A647-conjugated donkey anti-mouse antibody (1:1000) overnight at 4°C. Cultures were then washed twice with PBS and labeled with Hoechst 33342 (1:1000) for 20 minutes. After Hoechst labeling, the samples were washed three times with PBS. 25 fields were acquired for each well using a GE InCell Analyzer 2000 (GE Healthcare Life Sciences) at 10x magnification. The results are shown in Figure 19.

[0128] 2. Net neurite length Net neurite length was determined from the cultures described in the previous section. Neurite analysis was performed using a custom algorithm created with the GE InCell Investigator Developer Toolbox. Results from control and vehicle-treated samples were nearly identical and were combined for statistical analysis. Results are shown in Figure 20.

[0129] 3. Number and size of cortical spheres, length of processes, and branching Mouse C57 E14,15 cortices (Lonza:M-CX-300) were suspended in 12 mL of neurobasal medium supplemented with B27 and 2 mM Glutamax (Sigma-Aldrich). 200 μL was added to each well of a 96-well plate pre-coated with polylysine and laminin. After 4 days, 50% of the medium was replaced with fresh medium, and cells were treated with the test article (vehicle, PPF1, or HAS1) to a final concentration of 10%. This process was repeated 3 days later. On day 7 of treatment, phase-contrast images of cells were taken at 10x magnification using an IncuCyte (Ann Arbor, MI) and analyzed using the standard "Neurite and Cell-Body" algorithm. Six replicates were analyzed, with four images taken per replicate. Standard errors are shown. Significance is indicated as P<0.5 using a two-tailed t-test. Results are shown in Figures 21 and 22.

[0130] 4. Staining Neurospheres with Sox2 Mouse C57 E14,15 cortical neurons (Lonza:M-CX-300) were suspended at 100–200K cells / mL in neurobasal medium supplemented with B27 and 2 mM Glutamax (Sigma-Aldrich). 200 μL was added to each well of a 96-well plate pre-coated with collagen I (Corning, Inc.). After 16 h, the seeding medium was replaced with pre-warmed (37°C) control medium (neurobasal medium supplemented with B27 and 2 mM Glutamax (Gibco)). On day 4 of in vitro culture ("days in vitro" or "DIV"), the culture medium was replaced with fresh control medium, control medium containing HAS vehicle (vehicle), control medium plus 10% PPF1, or control medium plus 10% HAS1. Cultures were maintained for 21 days, with 75% of the medium replaced with fresh medium every 3–4 days. At 21 DIV, cultures were washed three times with PBS and then fixed with 4% paraformaldehyde for 20 minutes at room temperature (RT). After fixation, cultures were washed twice with PBS and then permeabilized with 0.1% Triton 100X for 5–20 minutes. After permeabilization, cultures were blocked with 3% bovine serum albumin (Sigma-Aldrich) for 60 minutes at RT. After 60 minutes, the blocking solution was aspirated, and cultures were labeled with anti-Tuj1 antibody (AbCam - 1:500) and rabbit anti-SOX2 (AbCam: 1:5000) overnight at 4°C. After labeling, cultures were washed three times with PBS + 0.1% BSA and then stained with donkey anti-mouse 647 (AbCam) and sheep anti-rabbit Texas Red (1:1000) overnight at 4°C. Cultures were then washed twice with PBS and labeled with Hoechst (1:1000) for 20 minutes. After Hoechst labeling, samples were washed three times with PBS. Using 10x magnification on an InCell Analyzer 2000 (GE Healthcare Life Sciences), 20 or 25 fields were acquired for each well. Analysis of neurospheres and neurites was performed using custom algorithms created with the GE InCell Investigator Developer Toolbox. Results from control and vehicle-treated samples were nearly identical and therefore combined for statistical analysis.The results are shown in FIG.

[0131] K. In vivo experimental results 1. Open field test using 3-month-old and 13-month-old NSG mice NSG mice aged 3 months (young) or 13 months (old) were placed in an open field chamber for 15 minutes. Rearing time (Figure 1), speed (Figure 2), and distance (Figure 3) were measured. Figure 1 shows that 13-month-old mice had less rearing time than 3-month-old mice, but PPF1- and HAS1-treated mice were not significantly different from young mice. Figure 2 shows that 13-month-old saline-treated (control) and PPF1-treated mice were significantly slower than 3-month-old mice. However, HAS1-treated mice were significantly faster than saline-treated mice and were not significantly different from young mice. Figure 3 shows that aged saline-treated (control) and HAS1-treated mice had lower locomotor activity than young mice, and PPF1-treated mice covered a longer distance than saline-treated mice. All data shown are mean ± sem. * P<0.05, ** P<0.01, *** P<0.001, t-test, n=20, 18, 18, 19 (SAL=saline).

[0132] 2. Y-maze test using 3-month-old and 13-month-old NSG mice Young (3-month-old) and aged (13-month-old) NSG mice were tested in a cued Y-maze as a test of memory. Figure 4 shows that all mice spent significantly more time in the novel (N) arm than the non-novel (F) arm. Figure 5 shows that HAS1-treated aged mice had significantly impaired memory for the non-novel arm compared to young mice, while PPF1-treated mice tended to have improved memory for the non-novel arm. Figure 6 shows that aged saline- and PPF1-treated mice were significantly slower than young mice, but HAS1-treated mice were not significantly different from young mice. Figure 7 shows that aged saline- and PPF1-treated mice covered shorter distances than young mice, but HAS1-treated mice were not significantly different from young mice. All data shown are mean ± sem. * P<0.05, ** P<0.01, *** *P<0.001, paired t test, n=20, 18, 18, 19 (SAL=saline).

[0133] 3. Fear conditioning test on memory using 3-month-old and 13-month-old NSG mice Young (3-month-old) and aged (13-month-old) NSG mice were tested in a fear conditioning memory test. Figure 8A shows that 13-month-old mice tended to freeze for shorter periods of time than 3-month-old mice, while HAS1-treated mice freezed for approximately the same amount of time as 3-month-old mice. Figure 8B shows that 13-month-old control-treated mice performed poorly and freezed for the shortest periods of time in the auditory cue memory test. HAS1-treated mice tended to freeze for longer periods of time, indicating improved memory for the sound. Figure 9 shows quantification of the final 90 seconds of the memory cue test, showing that HAS1-treated mice tended to freeze for longer periods of time, indicating improved memory. n = 20, 16, 17, 19 (SAL = saline).

[0134] 4. Barnes maze test for spatial memory in 3-month-old and 13-month-old NSG mice Young (3-month-old) and aged (13-month-old) NSG mice were tested in the Barnes maze test for spatial memory. Figure 10A shows that 3-month-old mice performed best, taking the shortest time to reach the target hole by the last trial. Figure 10B shows quantification of the average of the last three trials, indicating that target hole memory was significantly impaired in aged saline- and HAS1-treated mice compared with young mice, but not in PPF1-treated mice. ** P<0.01, *** P<0.001, unpaired t-test, n=20, 18, 18, 19 (SAL=saline).

[0135] 5. Immunostaining using 3-month-old and 13-month-old NSG mice Brain sections were stained for doublecortin (Dcx), a marker of newborn neurons, and Ki67, a marker of proliferating cells, from 3- and 13-month-old NSG mice treated twice weekly with saline, PPF1, or HAS1. Dcx- and Ki67-positive cells were counted in the dentate gyrus of young and aged NSG mice. Figures 11A and 11B show that all aged mice had dramatically lower numbers of Dcx- and Ki67-positive cells, respectively. PPF1- and HAS1-treated mice tended to have increased numbers of Dcx- and Ki67-positive cells compared with saline-treated mice.

[0136] 6. Immunostaining using 3-month-old and 13-month-old NSG mice treated with PPF1 and HAS1 three times a week Brain sections from 13-month-old mice were stained for doublecortin (Dcx), a marker of newborn neurons, and Ki67, a marker of proliferating cells. Mice were treated with saline, PPF1, 1x concentrated HAS1, or 5x concentrated HAS1 three times per week. Dcx- and Ki67-positive cells were counted in the dentate gyrus. Figure 12 shows that mice treated with PPF1 tended to have increased neurogenesis (as indicated by Dcx staining) compared with saline-treated control animals. It also shows that more concentrated HAS1 tended to increase neurogenesis compared with saline-treated animals.

[0137] Figure 13 shows that cell proliferation (as indicated by Ki67 staining) was significantly increased in mice treated with PPF1 compared to saline-treated control animals, and that more concentrated HAS1 tended to increase neurogenesis compared to saline-treated animals. * P<0.05, unpaired t-test versus saline group, all data shown are mean±sem.

[0138] 7. Open field test using NODscid mice NODscid mice were treated intravenously with either saline or PPF1 via tail vein injection twice weekly starting at 6 months of age. Each group initially contained 20 mice. Mice were placed in an open field chamber for 15 minutes, and locomotor activity was recorded. Figure 14A shows that PPF1-treated mice tended to have increased rearing activity compared to saline-treated mice. Figures 14B and 14C show that PPF1-treated mice also tended to have improved speed and distance covered, respectively, compared to saline-treated mice.

[0139] 8. Barnes maze using aged (12-month-old) NSG mice treated with young plasma, effluent I, and effluent II / III Aged NSG mice (12 months old) were divided into groups (all with a size of n = 14) and received 150 μL of saline, young plasma, effluent I, or effluent II / III via tail vein injection before the start of behavioral testing. Each separate group was further divided into three cohorts, and behavioral testing began in each cohort on a different week. Mice were tested in a modified Barnes maze (as described above) to assess spatial learning and memory. Figure 15 shows that treatment with young plasma, effluent I, or effluent II / III tended to significantly improve the latency of aged NSG mice to reach the target hole.

[0140] 9. Barnes maze and cell viability using young plasma and aged NSG mice treated with PPF1 As described above, aged male NSG mice (12 months old) were treated with 150 μL of purified young human plasma (young plasma), PPF1, or saline three times per week (iv) for 4 weeks and twice per week during weeks 5 and 6, the weeks in which the reported studies were performed.

[0141] Figure 16 shows the latency to reach the hole in the Barnes maze for each treatment cohort. Treatment with PPF1 significantly improved spatial memory in old mice compared to controls, and treatment with young plasma tended to improve spatial memory compared to controls (n: saline = 12, PPF1 = 14, young plasma = 11). * P<0.05, mean±sem, unpaired T-test.

[0142] Figure 17 shows the mean latency to find the target hole in the last three trials of each test day. Again, treatment with PPF1 significantly improved spatial memory in old mice compared to controls, and treatment with young plasma tended to improve spatial memory compared to controls. * P<0.05, mean±sem, unpaired T-test.

[0143] Figure 18 shows the effect of young human plasma and PPF1 on cell viability, as determined by the number of BrdU-positively labeled cells (i.e., proliferating cells) within the granular layer of the dentate gyrus in aged (12-month-old) NSG mice. BrdU was administered (ip) for 5 days prior to the start of intravenous injections of young plasma, PPF1, or saline control, as described above. A significant increase in cell viability was observed in both young human plasma- and PPF1-treated mice compared to saline control. Statistical significance was determined using one-way ANOVA with Dunnett's multiple comparison post-hoc analysis between PPF1 and young human plasma compared to saline treatment (n: saline = 13, PPF1 = 13, young plasma = 11, **** P > 0.0001, unpaired T test between PPF1 or young human plasma and saline treatment).

[0144] L. In vitro neurosphere and cortical culture assay results Figure 19 shows that PPF1 and HAS1 differentially regulate neurosphere proliferation in cortical cultures. E14-15 C57 mouse cortices were cultured on collagen I-coated 96-well plates in culture medium containing vehicle alone, PPF1 (10%), or HAS1 (10%). Example images of neurospheres in cortical cultures after 21 days of in vitro culture, imaged for Tuj1 (neuron-specific class III beta-tubulin), DAPI (4',6-diamidino-2-phenylindole), or both Tuj1 and DAPI, are shown. Figure 19 shows that PPF1 increases the amount of neurospheres expressing either Tuj1 or DAPI. The increased Tuj1 expression indicates that PPF1-treated cortical cultures produce more neurospheres that differentiate into a more neuronal-like phenotype.

[0145] Figure 20 shows three cultures of C57 mouse E14-15 cortical neurons (Lonza:M-CX-300) suspended at 100-200K cells / mL in neurobasal medium supplemented with B27, 2 mM Glutamax (Sigma-Aldrich), coated on collagen I-coated 96-well plates in culture medium containing vehicle, PPF1 (10%), or HAS1 (10%). Net neurite length, indicative of neurogenesis, was increased in PPF1-treated cultures compared to control or HAS1-treated cultures.

[0146] Figure 21 shows three cultures of E14-15 cortical neurons (Lonza:M-CX-300) from C57 mice, suspended at 100-200K cells / mL in neurobasal medium supplemented with B27 and 2 mM Glutamax (Sigma-Aldrich) coated on collagen I-coated 96-well plates in culture medium containing vehicle, PPF1 (10%), or HAS1 (10%). The IncuCyte software algorithm, available from Essen BioSciences (Ann Arbor, MI), detected cortical culture spheres (highlighted in yellow) and processes (highlighted in pink). More spheres and processes were observed in PPF1-treated cultures, and increased sphere size and process branching were also observed in PPF1-treated cultures. Scale bars are 300 μm.

[0147] Figures 22A-D show sphere number, process length, process branch points, and sphere size, respectively. Quantification was performed using the IncuCyte software algorithm available from Essen BioSciences (Ann Arbor, MI). Standard errors are shown. Significance using a two-tailed t-test is indicated. Figure 22A shows that PPF1-treated cultures had an increased number of spheres compared to vehicle- or HAS1-treated cultures (P=0.0006, PPF1 vs. vehicle; P=0.0007, PPF1 vs. HAS1). Figure 22B shows that PPF1-treated cultures displayed increased process length compared to vehicle- or HAS1-treated cultures (P=4e -8, PPF1 vs. vehicle, P=0.002, PPF1 vs. HAS1, and P=0.018, HAS1 vs. vehicle). Figure 22C shows that PPF1-treated cultures produced more process branch points compared to vehicle- or HAS1-treated cultures (P=0.002, PPF1 vs. vehicle, P=0.004, PPF1 vs. HAS1). Figure 22D shows that PPF1-treated cultures were associated with increased sphere size compared to vehicle- or HAS1-treated cultures (P=0.002, PPF1 vs. vehicle, P=0.004, PPF1 vs. HAS1). Collectively, the results of this data indicate that treatment with PPF1 (and, to a lesser extent, treatment with HAS1) is associated with features indicative of increased cell growth and process formation in cortical cultures.

[0148] Figure 23 shows the number of neurospheres staining positive for Sox2, a transcription factor that plays an important role in the maintenance of embryonic and neural stem cells. Quantification was performed using an algorithm in the GE InCell Investigator Toolbox. PPF1-treated cultures significantly increased the number of neurospheres staining positive for Sox2, indicating that PPF1 treatment is associated with an increase in the number of cells with neurogenic potential.

[0149] The foregoing merely illustrates the principles of the present invention. It will be appreciated that those skilled in the art will be able to devise various configurations that embody the principles of the present invention and are within its spirit and scope, even if not explicitly described or presented herein. Furthermore, all examples and conditional language recited herein are intended primarily to aid the reader in understanding the principles and concepts of the present invention, which the inventors have contributed to advancing the art, and should not be construed as being limited to such specifically recited examples and conditions. Furthermore, all statements herein reciting the principles, aspects, and embodiments of the present invention, as well as specific examples thereof, are intended to encompass both structural and functional equivalents. Furthermore, such equivalents are intended to include both currently known equivalents and future-developed equivalents, i.e., any elements developed to perform the same function, regardless of structure. Therefore, the scope of the present invention is not intended to be limited to the exemplary embodiments shown and described herein. Rather, the scope and spirit of the present invention are embodied by the appended claims.

[0150] CROSS-REFERENCE TO RELATED APPLICATIONS This application claims priority under 35 U.S.C. §119(e) to the filing date of U.S. Provisional Patent Application No. 62 / 412,258, filed October 24, 2016, the disclosure of which is incorporated herein by reference.

Claims

1. A composition for treating age-related cognitive impairment, comprising: comprising an effective amount of a plasma fraction for administration to a subject diagnosed with age-related cognitive impairment, The plasma fraction is a human albumin solution (HAS) containing at least 95% albumin and not more than 5% globulins and other plasma proteins. composition.

2. 10. The composition of claim 1, wherein the HAS is a commercially available HAS.

3. 3. The composition of claim 1 or 2, wherein the subject is monitored for improvement in cognitive function.

4. The composition of any one of claims 1 to 3, wherein the HAS is derived from plasma from a pool of people under the age of 40.

5. The composition of any one of claims 1 to 4, wherein the subject is a mammal.

6. The composition of claim 5 , wherein the mammal is a human.

7. 1. A kit for use in treating age-related cognitive impairment in a subject, comprising: A kit comprising a container containing the plasma fraction according to any one of claims 1 to 6.

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