Pest repellent and method for repelling pests

Abstract

To provide a household pest repellent that employs components included in natural products, has sufficient repelling effect on household pests, is highly safe for human bodies, and can be used conveniently even in general households.SOLUTION: A household pest repellent comprises pressed juice, extract or distillate of a plant from the Bidens genus in the Asteraceae family, as an active ingredient.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention relates to a repellent for and a method for repelling pests of living insects, and more particularly to a repellent for and a method for repelling pests of living insects that have an excellent repellent effect against pests that pest textiles and pests that pest grains in the living environment. [Background technology]

[0002] BACKGROUND ART Various insect repellents for insects that cause daily life have been proposed, and pyrethroid compounds and the like are widely used as insect repellent components.

[0003] In recent years, there has been a demand for the use of safe natural ingredients and food ingredients as insect repellent ingredients. For example, known examples include a miticide in which cedar essential oil, obtained by steam extraction of cedar branches and leaves, is extracted with a hydrous polar organic solvent (Patent Document 1), and a mite control agent for sanitary pests that contains as an active ingredient at least one of essential oils extracted from the branches, leaves, or roots of Japanese cypress, Taiwan cypress, Japanese cedar, and Japanese cypress, or a mixture of these essential oils.

[0004] The applicant has also disclosed an insect repellent against textile pests (Patent Document 3) and mites (Patent Document 4), which contains as an active ingredient a squeezed liquid, extract, or distillate of a plant belonging to the genus Abies of the family Pinaceae.

[0005] In addition, control agents for agricultural pests such as nematodes (Patent Documents 5 and 6) and armyworm moth (Patent Document 7) are known which contain extracts of plants of the Asteraceae family, such as Bidens frondosa, as active ingredients. [Prior art documents] [Patent documents]

[0006] [Patent Document 1] Japanese Patent Application Publication No. 6-239714 [Patent Document 2] Japanese Patent Application Publication No. Hei 1-193204 [Patent Document 3] Japanese Patent Application Laid-Open No. 2015-157780 [Patent Document 4] Japanese Patent Application Publication No. 2017-39677 [Patent Document 5] Japanese Patent Application Laid-Open No. 2008-120749 [Patent Document 6] Japanese Patent Application Publication No. 2019-202978 [Patent Document 7] Japanese Patent Application Laid-Open No. 2010-208994 Summary of the Invention [Problem to be solved by the invention]

[0007] To provide a pest repellent which utilizes components contained in natural products, has an excellent repellent effect against pests, is highly safe for humans, and can be easily used in ordinary households. [Means for solving the problem]

[0008] In order to achieve the above object, the present inventors have been conducting extensive research into the activity of components in natural products against living pests, and have discovered that compressed liquids, extracts, or distillates of certain Asteraceae plants exhibit excellent repellent effects, leading to the completion of the present invention.

[0009] That is, the present invention relates to a pest repellent containing, as an active ingredient, a squeezed liquid, extract or distillate of a plant of the genus Bidens of the Asteraceae family.

[0010] The present invention also relates to a method for repelling living pests using the above-mentioned living pest repellent. [Effects of the Invention]

[0011] The pest repellent of the present invention has an excellent repellent effect against a wide range of pests, and is extremely safe because it is a component of a natural product. Therefore, it can be easily used in ordinary households and can effectively prevent damage caused by various pests. [Brief explanation of the drawings]

[0012] [Figure 1] FIG. 1 is a diagram of a microwave distillation apparatus. [Figure 2] FIG. 1 is a diagram schematically illustrating a cross section of a test device used in Example 1. [Figure 3] FIG. 2 is a diagram schematically illustrating a plane of a test device used in Example 1. [Figure 4] FIG. 2 is a schematic cross-sectional view of a test device used in Examples 2 and 3. [Figure 5] FIG. 2 is a diagram showing a schematic plan view of a passage pipe of a test device used in Examples 2 and 3. DETAILED DESCRIPTION OF THE INVENTION

[0013] The pest repellent of the present invention contains as an active ingredient a squeezed liquid, extract, or distillate of a plant of the genus Bidens in the Asteraceae family. Examples of plants of the genus Bidens in the Asteraceae family include Bidens pilosa L. and its variants or cultivars. Specific examples of varieties include Bidens pilosa var. radiata Scherff and Bidens pilosa L. var. radiata Sch. Bip., and examples of cultivars include Bidens pilosa L. var. radiata Scherff. f. decumbens Scherff.. Among these plants of the genus Bidens in the Asteraceae family, Bidens pilosa, fleabane, and ragweed are preferred in terms of their repellent effect against pests, with Bidens pilosa being particularly preferred.

[0014] The method for obtaining a squeezed liquid, extract, or distillate from the above-mentioned plants of the genus Bidens of the Asteraceae family is not particularly limited, and known methods can be used. For example, squeezed liquid can be produced using a known compressor or the like. The extract can also be produced according to a conventional method, but polar solvents are preferred as the extraction solvent. Specific examples include alcoholic solvents such as water, ethanol, methanol, butyl alcohol, ethylene glycol, propylene glycol, and glycerin; halogen-containing solvents such as chloroform; ether solvents such as dioxane; carbonyl solvents such as acetone and ethyl acetate; and mixtures thereof. The distillate can be obtained by distillation methods such as atmospheric distillation, reduced-pressure distillation, and steam distillation.

[0015] One method for producing the extract involves first obtaining the whole plant or a specific part of a plant of the genus Bidens (Asteraceae), either fresh immediately after harvest or dried in a dryer or the like, either as is or cut, crushed, or finely powdered, and then adding an aqueous solvent such as water or a mixed solution of water and a lower alcohol, which is 5 to 250 times the mass of the plant of the genus Bidens (Asteraceae), extracting for 10 minutes to several days at room temperature or near the boiling point of the solvent, and filtering the extract. Examples of parts of the plant of the genus Bidens (Asteraceae) include flowers, leaves, stems, and roots, and one or more of these parts can be used for extraction. Preferably, the whole plant excluding the roots is used, and more preferably, the leaves.

[0016] For example, when water is used as the aqueous solvent, an extract can be obtained by finely chopping fresh or dried flowers, leaves, stems, roots, or the whole plant of a plant of the genus Bidens of the Asteraceae family, adding water in an amount approximately 50 times its mass, heating, and maintaining the boiling state for approximately 30 minutes.

[0017] Furthermore, when using a mixed solution consisting of 30% by mass of water (hereinafter simply referred to as "%)" and 70% ethanol as the aqueous solvent, an extract can be obtained by finely chopping fresh or dried flowers, leaves, stems, roots or the whole plant of a Bidens genus plant of the Asteraceae family, adding approximately 15 times the mass of the mixed solution, and leaving it to stand at 25°C for 10 days for extraction.

[0018] The distillate can be produced by heating or distilling the whole plant or specific parts of a plant of the genus Bidens of the Asteraceae family, either fresh immediately after harvest or dried in a dryer, either as is or after cutting, crushing, or powdering, at reduced or normal pressure. It is preferable to distill using only the water contained in the plant without adding water. The distillate obtained may be used as is, but it is also preferable to separate and use the aqueous fraction.

[0019] Although heating during distillation can be performed using a general heater or the like, microwave heating is preferred in terms of heating efficiency, and it is particularly preferred to heat by irradiating with microwaves without adding water. By irradiating with microwaves, water molecules contained in the plant are directly heated to generate water vapor, which acts as a mobile phase to extract the volatile components in the plant. Therefore, this extraction method can be considered to include elements of reduced pressure distillation using the boiling points of the volatile components and elements of steam distillation.

[0020] Such a distillate by microwave irradiation can be obtained, for example, by using the apparatus shown in Figure 1. In the figure, 1 is a microwave distillation apparatus, 2 is a distillation tank, 3 is a microwave heating device, 4 is a stirring bar, 5 is an air inlet pipe, 6 is a distillate outlet pipe, 7 is a cooling device, 8 is a heating control device, 9 is a vacuum pump, 10 is a pressure regulating valve, 11 is a pressure control device, 12 is a distillation target, and 13 is a distillate.

[0021] In this apparatus 1, the raw material, a plant of the genus Bidens of the Asteraceae family, which serves as the distillation target 12, is placed in a distillation tank 2, and while stirring with stirring blades 4, microwaves are radiated from a microwave heating device 3 mounted on the top surface of the distillation tank 2 to heat the raw material. The distillation tank 2 is connected to an air flow inlet 5 and a distillate outlet pipe 6. The air flow inlet pipe 5 introduces air or an inert gas such as nitrogen gas into the distillation tank 2, and this air flow is introduced from the bottom of the distillation tank 2. The distillate outlet pipe 6 discharges the distillate from the raw material from the top of the distillation tank 2 to the outside.

[0022] The temperature and pressure inside the distillation tank 2 are measured by a temperature sensor and a pressure sensor (neither shown) attached thereto, and are adjusted via a heating control device 8, a pressure control device 11, and a pressure regulating valve 10, respectively.

[0023] The gaseous extract flowing out of the distillation tank 2 via the distillate outlet pipe 6 is converted into a liquid by a cooling device 7, and is obtained as a distillate 13. This distillate 13 contains an oily fraction 13a and an aqueous fraction 13b, of which the aqueous fraction 13b is preferably used.

[0024] The distillation should be carried out at a pressure of 3 to 110 kPa, preferably about 3 to 100 kPa, within the distillation tank 2, resulting in a vapor temperature of 40 to 100°C. At a pressure of less than 3 kPa, the increase in vapor pressure of the volatile components in the plant is suppressed, and the vacuum distillation effect due to the boiling points of each component becomes dominant rather than the steam distillation effect, resulting in components with lower boiling points escaping first. This can result in less efficient extraction of components with higher boiling points than water, or in the extraction of a large amount of high-boiling components that have lower insect repellent efficacy and less palatable fragrances. Furthermore, at pressures above 110 kPa, the raw material temperature rises, resulting in significant energy loss and potentially accelerating oxidation of the raw material.

[0025] The distillation time should be about 0.2 to 8 hours, preferably about 0.4 to 6 hours. If it is less than 0.2 hours, many unextracted components from the plant remain, while if it is more than 8 hours, the raw material becomes nearly dry, which is undesirable in that it reduces the extraction efficiency.

[0026] Furthermore, the gas introduced into distillation tank 2 may be air, but an inert gas such as nitrogen gas, helium gas, or argon gas is preferred, and the flow rate thereof may be set to about 0.001 to 0.1 times the volume of extraction tank 2 per minute.

[0027] The distillate obtained by this method is colorless and transparent, and is therefore advantageous in that it can be used directly for applications such as clothing and fabric products.

[0028] The distillate extracted from a plant of the genus Bidens of the Asteraceae family as described above can be used as is, but if necessary, it may be further purified by filtration or other means within a range that does not affect its efficacy. Such purification may be carried out by any conventional means selected as appropriate, and examples thereof include filtration using filter paper.

[0029] The pest repellent of the present invention may be in a form in which the above-mentioned component is used alone as the active ingredient, or may be in a form in which it is further combined with any suitable optional component in a conventional manner and used as a formulation.

[0030] The pest repellent of the present invention can be used by evaporation or contact with pests, as described below, and contains an active ingredient in an amount appropriate for the method. For example, it is preferable to incorporate the active ingredient in the pest repellent so that the solid content is 0.1 to 99.99%, more preferably 1 to 99.9%, and particularly preferably 10 to 90%.

[0031] The formulation of the pest repellent of the present invention is not particularly limited, and it can be in the form of a liquid, gel, solid, etc., and any optional ingredients can be used accordingly.

[0032] One of the formulations of the pest repellent of the present invention is a liquid formulation, and a solvent is used to prepare this liquid formulation. The solvent to be used is not particularly limited, and any conventionally known liquid carrier can be used, but it is preferable to use one that is highly safe for the body.

[0033] Specific examples of the solvent include water such as deionized water and extraction water; hydrocarbon compounds such as hexane, paraffin, and isoparaffin; alkyl alcohols such as methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, 2-methyl-1-propanol, 2-methyl-2-propanol, pentanol, hexanol, heptanol, octanol, nonanol, and decanol; alkenyl alcohols such as allyl alcohol; aromatic ring-containing alcohols such as benzyl alcohol; phenols such as eugenol; and chloroform. ether compounds such as diethyl ether and diethylene glycol diethyl ether; ketone compounds such as acetone; fatty acid compounds such as acetic acid and oleic acid; ester compounds such as acetate esters, propionate esters and benzoate esters; glycol compounds such as ethylene glycol and propylene glycol; glycol ether compounds such as 2-phenoxyethanol and 3-methyl-3-methoxybutanol; and vegetable oils such as sesame oil, linoleic oil and salad oil, and these can be used alone or in combination.

[0034] The amount of these solvents used in the living pest repellent is preferably 0.1 to 99.99%, more preferably 1 to 99.9%, and even more preferably 10 to 90%, based on the total amount of the living pest repellent.

[0035] In preparing this liquid preparation, a surfactant may be added as needed. Usable surfactants include conventionally known surfactants such as cationic surfactants, anionic surfactants, amphoteric surfactants, and nonionic surfactants, and these may be used alone or in combination of two or more.

[0036] Another formulation of the pest repellent of the present invention is a solid or sheet-like formulation obtained by impregnating a suitable carrier with the liquid formulation and supporting it. The carrier is not particularly limited, but examples thereof include wood, paper, woven fabric, nonwoven fabric, silica, talc, activated carbon, silica gel, zeolite, cellulose beads, ceramic, and resin pellets such as polypropylene.

[0037] Another example of the formulation of the pest repellent of the present invention is a gel obtained by gelling the liquid formulation with a gelling agent. Examples of gelling agents that can be used include conventionally known ones, such as carrageenan, gellan gum, agar, gelatin, guar gum, pectin, locust bean gum, xanthan gum, sodium alginate, cellulose derivatives, dibenzylidene-D-sorbitol, hydroxypropylated polysaccharides, inulin stearate, superabsorbent resins such as sodium acrylate, and aluminum octylate, and these can be used alone or in combination of two or more.

[0038] A preferred method of using the living pest repellent of the present invention obtained as described above is to release the living pest repellent of the present invention into the environment.

[0039] Examples of methods for releasing into the environment include a method in which the active ingredient of the living pest repellent of the present invention, such as a liquid formulation, a solid formulation, or a gel formulation, is naturally evaporated at room temperature, or a method in which the active ingredient is forced to evaporate. Methods for forced evaporation include evaporation by heating, evaporation by blowing air, and evaporation by fumigation. Alternatively, a method of spraying or the like may be used as a method for releasing into the environment.

[0040] Among the above-mentioned forced evaporation methods, examples of evaporation methods using heat include a method using a heating evaporator, as disclosed in Japanese Utility Model Laid-Open Publication No. 2-78077, in which a pest repellent stored in a liquid bottle is evaporated by wicking the pest repellent with a wick and heating the upper part of the wick with a heater, and a heating evaporator, as disclosed in Japanese Utility Model Laid-Open Publication No. 1-116072, in which a mat-shaped impregnated body is impregnated with the pest repellent and the mat is placed on a heater to evaporate the pest repellent.

[0041] In addition, evaporation by blowing air can be carried out using a fan-type insect control device, such as that disclosed in Japanese Patent Application Laid-Open No. 11-308955, which is equipped with a chemical agent holder and a blower, and which releases the pest repellent into the environment from an outlet by bringing the chemical agent holder into contact with the airflow generated by the blower.

[0042] Furthermore, evaporation by fumigation can be carried out using a fumigation device, such as that disclosed in Japanese Utility Model Application Laid-Open Publication No. 148267 / 1979, in which a storage chamber is formed in an open-topped container containing an organic foaming agent such as an azo compound such as azodicarbonamide, a nitroso compound, or a hydrazide compound, and a pest repellent, and the bottom of the storage chamber is heated by a heating means to decompose the foaming agent and generate nitrogen gas, carbon dioxide gas, etc., which evaporates the pest repellent.

[0043] Furthermore, spraying can be carried out by using a sprayer to spray a liquid agent or by using an aerosol with an appropriate propellant. When a spray agent is used, the liquid agent can be combined with a spraying means such as a trigger spray or an atomizer. When an aerosol agent is used, the liquid agent can be combined with an aerosol carrier such as LPG or isopentane and an aerosol spraying means such as an aerosol can.

[0044] Another preferred method of using the pest repellent of the present invention is to contact pests with the pest repellent in liquid or solid form. In this case, the repellent exerts its repellent effect when pests come into contact with the installed pest repellent. For example, the repellent may be applied directly to bedding such as futons, pillows, and blankets, tatami mats, carpets, sofas, cushions, stuffed toys, and clothing, or to places or objects where mites live, or may be sprayed using a pump sprayer, aerosol sprayer, or the like.

[0045] The pest repellent of the present invention can be placed and applied to storage containers such as storage rooms, dressers, closets, wardrobes, clothing cases, clothing storage bags, futon storage bags, kitchens, food storage containers, rice storage containers, cupboards, trash cans, living rooms, bedrooms, entranceways, garbage collection areas, etc., whereby the active ingredient is released into the environment or comes into contact with pests, thereby effectively repelling pests.The pest repellent of the present invention can also be applied by directly spraying, sprinkling, or coating to objects where pests live, including tatami mats, rugs, carpets, floors, bedding such as futons and pillows, sofas, and textile products such as clothing.

[0046] The pest repellent of the present invention may contain other known insect repellent ingredients, which can provide even better insect repellent effects.

[0047] Examples of these other insect repellent ingredients include pyrethroid compounds such as natural pyrethrins, empenthrin, transfluthrin, allethrin, fenothrin, profluthrin, metofluthrin, and eminence; organophosphates such as dichlorvos, diazinon, fenitrothion, and malathion; N,N-diethyl-m-toluamide (DEET), dimethyl phthalate, dibutyl phthalate, 2-ethyl-1,3-hexanediol, di-n-propyl isocinchomeronate, p-dichlorobenzene, and di-n-butyl sucrose. Cuccinate, carane-3,4-diol, 1-methylpropyl 2-(2-hydroxyethyl)-1-piperidinecarboxylate, isopropyl myristate, isobornyl thiocyanoacetate, diphenyl, diphenylmethane, dibenzyl, benzyl phenyl ether, benzyl phenyl ethyl ether, benzophenone, benzyl phenyl ketone, dibenzyl ketone, benzalacetophenone, β-phenylethyl benzoate, γ-phenylpropyl benzoate, phenyl phenylacetate, benzyl phenylacetate acetate, β-phenylethyl phenylacetate, phenyl cinnamate, benzyl cinnamate, β-phenylethyl cinnamate, β-phenylpropyl cinnamate, cinnamyl cinnamate, diphenyl carbinol, phenylbenzyl carbinol, dibenzyl carbinol, n-amyl benzoate, isoamyl benzoate, hexyl benzoate, heptyl benzoate, octyl benzoate, nonyl benzoate, cis-3-hexenyl benzoate, n-amyl salicylate, iso-amyl Compounds such as myrsalicylate, hexyl salicylate, cis-3-hexenyl salicylate, benzyl propionate, benzyl-n-butyrate, benzoylisobutyrate, benzyl-n-valerate, benzyl isovalerate, benzyl caproate, benzyl heptanoate, benzyl caprylate, benzyl danilate, eugenol, methyl eugenol, isoeugenol, etc.; terpene compounds such as α,β-pinene, α,β-terpinol, limonene, citronellal, linalool, citral, etc.;Examples of the active ingredient include one or more selected from the group consisting of menthol, vetiver oil, patchouli oil, phellandrene, basil oil, shell ginger oil, clove leaf oil, black pepper oil, acetophenone, allyl isothiocyanate compounds, and the compressed liquid, extract, or extract of a plant belonging to the genus Abies in the family Pinaceae, and these may be used alone or in combination of two or more;

[0048] The pest repellent of the present invention as described above has a repellent effect against various pests that occur in homes. There is no particular limitation on the pests that the pest repellent of the present invention is effective against, but specific examples include the cigarette beetle, the red flour beetle, the flat-headed red flour beetle, the sawtooth flathead beetle, the large rice flathead beetle, the rice weevil, the rice weevil, the grain boston beetle, the granary rice weevil, the small cutworm beetle, the small cutworm beetle, and the Japanese kakumunehime. Coleoptera pests such as cutworms; Lepidoptera pests such as the Japanese burrowing moth, Indian meal moth, striped meal moth, wax moth, Japanese honeysuckle moth, dream moth, bur moth, and Japanese box moth; Thymiidae pests such as Lepisma saccharina; Orthoptera pests such as the oriental cockroach, American cockroach, Leucophaea maderae, German cockroach, and Achata domesticus; Phorphycra Dermaptera pests such as Aedes auricularis;Termite pests such as Reticulitermes;Phthiraptera pests such as body lice;Hemiptera pests such as bedbugs, Rhodnius prolixus, and Triatoma infestans;Hymenoptera pests such as house ants, Lasius niger, and wasps;Aedes aegypti, Anopheles, Culex, house flies, and fruit flies Examples include Diptera pests such as house flies, little house flies, blow flies, blow flies, blowflies, stable flies, and horseflies; Siphonaptera pests such as cat fleas, human fleas, rat fleas, and long-legged fleas; and Acari pests such as Dermatophagoides farinae, Dermatophagoides pteronyssinus, Tyrophagus putrescentiae, Sarcoptes scabiei, ixodid mites, house mites, cheyletid mites, and Cheyletidae, including both adult and larval forms of these. Among these, pests that are suitable for use against Coleoptera pests, Lepidoptera pests, Orthoptera pests, Termitera pests, Phthiraptera pests, Hemiptera pests, Acarina pests, and Diptera pests are preferred because of their excellent repellent effect. It also has an excellent repellent effect against textile pests such as burr moths, rice moths, lesser cutworm beetles, lesser cutworm beetles, and silverfish; adult grain pests such as Indian meal moths, mealybugs, cigarette beetles, maize weevils, rice weevils, and red flour beetles; house dust mites such as Dermatophagoides farinae and Dermatophagoides pteronyssinus; and pests such as Drosophila melanogaster, and it is particularly effective in repellent of textile pests and grain pests. [Example]

[0049] The present invention will now be described in more detail with reference to examples, but the present invention is not limited to these examples in any way.

[0050] Manufacturing Example 1 The stems, leaves, flowers, and seeds of Bidens frondosa were dried for 24 hours at 100°C in a dry heat sterilizer, then cut with scissors and finely pulverized in a mixer. 250g of this pulverized material was placed in a 2L beaker, and 1250ml of distilled water was added and mixed thoroughly. The mixture was covered with plastic wrap and then with aluminum foil to prevent evaporation. The mixture was boiled for 30 minutes, cooled to room temperature (25°C), and filtered through cotton cloth to obtain an extract of a plant of the Asteraceae family (Product 1 of the present invention). The resulting extract was brownish in color.

[0051] Manufacturing example 2 The stems, leaves, flowers, and seeds of Bidens frondosa were cut with scissors and then finely ground in a mixer. Approximately 400 g of this material was placed in a 21 cm x 11 cm x 14 cm glass container. The opening was sealed with a silicone lid equipped with a cock, and the container was maintained under a pressure of approximately 11 kPa. Microwaves were irradiated to a heating temperature of 60°C, and distillation was performed for 15 minutes. After extraction, the liquid in the glass container was filtered to separate the aqueous fraction (Product 2 of the present invention). The resulting aqueous fraction was colorless and transparent.

[0052] Manufacturing Example 3 The stems, leaves, flowers, and seeds of Bidens frondosa were cut with scissors and then finely ground in a mixer. Approximately 400 g of this material was placed in a 21 cm x 11 cm x 14 cm glass container. The opening was sealed with a silicone lid equipped with a cock, and the container was maintained under a pressure of approximately 101 kPa. Microwaves were irradiated to a heating temperature of 100°C, and distillation was performed for 20 minutes. After extraction, the liquid in the glass container was filtered to separate the aqueous fraction (Product 3 of the present invention). The resulting aqueous fraction was colorless and transparent.

[0053] Manufacturing Example 4 Approximately 200 g of raw materials (stalks, leaves, flowers, and seeds of Bidens frondosa) were cut with scissors and finely ground in a mixer. These materials and 400 g of the aqueous fraction of Invention Product 3 produced in Production Example 3 above were placed in a 21 cm x 11 cm x 14 cm glass container. The opening was sealed with a silicone cap equipped with a stopcock, and the container was maintained under a pressure of approximately 101 kPa. Microwave irradiation was performed to heat the container to a temperature of 100°C, and distillation was carried out for 35 minutes. After distillation, the liquid in the glass container was filtered, and the aqueous fraction was separated to obtain Invention Product 4. The resulting aqueous fraction was colorless and transparent.

[0054] Manufacturing Example 5 The stems, leaves, flowers, and seeds of Bidens pilosa were used as raw materials, and an aqueous fraction was obtained as follows: About 50 kg of Bidens pilosa crushed in a crushing mill (KYB Corporation) was placed in the distillation tank of the microwave distillation apparatus shown in Figure 1. The pressure in the distillation tank was maintained at a reduced pressure of about 20 KPa (vapor temperature about 67°C) while stirring, and microwave irradiation was performed for 1 hour to distill the mixture. The aqueous fraction (Product 5 of the present invention) was separated from the distillate.

[0055] Example 1 Repellency test against Dermatophagoides farinae: In accordance with JIS L 1920 "Testing method for anti-mite performance of textile products," mites were reared and their density was adjusted, and repellency tests were conducted using the equipment shown in Figures 2 and 3.

[0056] Specifically, the samples were prepared by diluting Products 1 to 3 with ethanol to 1%. 60 mg of each sample was applied to a 40 mm diameter cotton cloth to obtain a test piece. As shown in Figures 2 and 3, the test piece (23) was placed in a small glass Petri dish (22) with a diameter of 45 mm and a height of 17 mm, and 0.05 g of attractant feed was placed in the center. A mite culture medium (21) containing approximately 10,000 live house dust mites (Dermatophagoides farinae) (a 1:1 mixture of powdered feed for rearing medium and dry yeast, by mass) was evenly spread on a large plastic Petri dish (20) with a diameter of 90 mm and a height of 14 mm, and the small glass Petri dish (22) was placed in the center. The resulting dish was then placed in a plastic container (24) containing saturated saline (25) to control humidity.

[0057] The container was then sealed with a lid and placed in a room at a temperature of 25°C ± 1°C. After leaving it for 20 hours, the total number of surviving mites on the test piece and the attractant medium was counted. This test was repeated three times, using cotton cloth that had not been treated with any pesticide as a blank. The total number counted in the three tests was calculated, and the repellency rate was calculated using the following formula. The mortality rate was also calculated using the following formula. The results are also shown in Table 1.

[0058] Repellency rate (%) = {(A1-A2) / A1} x 100 Mortality rate (%)=(A3 / A2)×100 A1: Number of mites on blank cotton cloth and on the attractant medium A2: Number of mites on the test specimens and on the attractant medium in the treated area A3: Number of dead mites on the test specimens and on the attractant medium in the treated area

[0059] [Table 1]

[0060] The above results demonstrate that the product of the present invention has a high repellent effect against Dermatophagoides farinae. On the other hand, since the mortality rate of both species is low, it has been confirmed that when applied to bedding, carpets, etc., dead bodies of the dust mites, which are allergens, are less likely to remain on the bedding, carpets, etc., and that the product also has an excellent allergen removal effect.

[0061] Example 2 Repellency test against rice weevils: The present invention products 1 to 3 were examined for their repellent effect against adult maize weevils, which are pests on grains, using the test equipment shown in FIGS.

[0062] <Test equipment> As shown in Figure 4, the test apparatus 30 is configured so that the test area 31 and the control area 32 (each a polypropylene cylindrical container with an inner diameter of approximately 85 mm, a depth of 110 mm, and a capacity of 570 ml) are connected by a passage pipe 33 (inner diameter 12 mm, length 50 mm, thickness 3 mm), allowing the test pests to move freely between the two areas. A 4.6 cm diameter Petri dish 34 is placed in the test area 31 of this test apparatus, and a Petri dish 34 of the same shape as the one placed in the test area 31 is placed in the control area 32. Furthermore, as shown in Figure 5, a 30 mm x 20 mm air hole 36 (covered with nylon mesh, so that the test pests cannot pass through) is formed above the passage pipe 33 of the test apparatus 30, preventing the volatilized insect repellent from traveling up the passage pipe 33 to the control area 32.

[0063] <Adult insect repellency test method> In an insect control test using the above-mentioned testing apparatus, first, the lids 37 of the test area 31 and the control area 32 were opened, and 1.0 g of brown rice (Akita Komachi) was placed as grain in each petri dish 34, which was then placed in the position shown in Figure 4. Next, 100 μl of each of the present inventions 1 to 3 was impregnated into 3 cm x 3 cm filter paper, and the impregnated paper was placed in a stainless steel cage 35 and placed in the test area 31. Then, 10 adult maize weevils, the target pest, were placed in each of the test area 31 and the control area 32, for a total of 20 adults.

[0064] The lids 37 of both plots were then closed, and the test apparatus 30 was left standing at 25°C and a relative humidity of 70%RH. After 10 minutes, 1 hour, 2 hours, and 3 hours, the number of adult insects in the test plot 31 was counted, and the ratio of the adult insects present in the test plot 31 to the initial number in the test plot (repellency rate) was calculated according to the following formula. The results are shown in Table 2.

number

[0065] [Table 2]

[0066] From the above results, it was confirmed that the product of the present invention has a high repellent effect against maize weevils, which are pests of grains.

[0067] Example 3 Repellency test against Indian meal moth: The repellent effect of Product 2 of the present invention on adult Indian meal moths was examined after 1 hour, 2 hours, and 1 day using the same method as in Example 2. The results are shown in Table 3.

[0068] [Table 3]

[0069] It was confirmed that invention product 2 has a repellent effect against the Indian meal moth, a textile pest.

[0070] Example 4 Drosophila repellency test: The product of the present invention 2 was diluted 10 times with distilled water and used as a sample, and the repellent effect on adult fruit flies was examined in accordance with the method of Example 2.

[0071] The test apparatus used was the same as that shown in FIG. 4 except that the petri dish 34 and the stainless steel basket 35 were removed. First, the lids 37 of the test area 31 and the control area 32 were opened, and 2 g of bananas ("Yasashisa Banana" from the Philippines) were placed in each area. Next, 100 μl of a sample of Product 2 of the present invention diluted 10 times with distilled water was impregnated into absorbent cotton and placed in the test area 31. Next, 10 adult fruit flies, the target pest, were placed in each of the test area 31 and the control area 32, for a total of 20 flies. Thereafter, the lids 37 of both areas were closed, and the test apparatus 30 was left to stand at 25°C and a relative humidity of 70% RH. The repellency rates after 10 minutes, 2 hours, and 3 hours were calculated in the same manner as in Example 2. The results are shown in Table 4.

[0072] [Table 4]

[0073] It was confirmed that Product 2 of the present invention has a repellent effect on adult fruit flies.

[0074] Formulation Example 1 Gel-type insect repellent: 85 g of isoparaffin was mixed with 10 g of an ethanol solution containing 1% of Invention Product 2, to which 5 g of aluminum octylate was added, and the mixture was heated to 60°C and stirred. The mixture was then filled into a container measuring 35 mm in length, 35 mm in width, and 75 mm in height and allowed to stand at room temperature for 3 hours to obtain a gel-like insect repellent.

[0075] Formulation Example 2 Room temperature volatile insect repellent: A 30mm x 40mm pulp filter paper was impregnated with 0.6g of an ethanol solution containing 1% of the present invention product 2, and this was placed in a breathable plastic case (38mm x 52mm x 5mm) with a 9mm x 22mm ventilation hole to prepare a pest repellent.

[0076] Formulation Example 3 Aerosols: Aerosol preparations were prepared by filling a commercially available 200 ml aerosol can with the composition of the following formulation. Active ingredient: 1.5ml of this invention's product 2 Solvent: ethanol 38.6 ml Propellant: LPG 30.0ml Propellant: Isopentane 30.0ml

[0077] Formulation Example 4 Spray: The composition having the following formulation was placed in a commercially available 150 ml Trigg container to prepare a spray. Active ingredient: 1g of this invention's product 2 : Todomatsu essential oil 1g Solvent: ethanol 10g Fragrance 0.05g Surfactant 0.1g Ion-exchanged water 88.75g

[0078] Formulation Example 5 Spray: The composition having the following formulation was placed in a commercially available 150 ml Trigg container to prepare a spray. Active ingredient: 1g of this invention : Natural pyrethrin 0.1g Solvent: ethanol 10g Fragrance 0.05g Surfactant 0.1g Ion-exchanged water 88.75g

[0079] Formulation Example 6 Fumigants: 10 g of an ethanol solution containing 1% of Invention Product 1, 55 g of azodicarbonamide, and 35 g of talc were kneaded together, and the mixture was granulated and dried in a conventional manner to prepare a fumigant.

[0080] Formulation Example 7 Heat evaporation agent: A pulp mat measuring 3 cm x 3 cm was impregnated with 100 mg of an ethanol solution containing 1% of Invention Product 1 to obtain a thermal evaporation agent. This preparation evaporates the active ingredient when placed in contact with a heater.

[0081] Formulation Example 8 Granular Living Pest Repellent: 50 g of pulp granular impregnated material (average particle size 5 mm) was impregnated with 50 mL of an ethanol solution containing 1% of invention product 2, and placed in a breathable nonwoven bag to obtain a granular repellent for fabric pests and household pests.

[0082] Formulation Example 9 Room temperature volatile insect repellent: A 9.6mm x 110mm pulp filter paper was impregnated with 0.5g of an ethanol solution containing 1% of Product 2 of the present invention and 0.01g of empenthrin, and placed in a 88mm x 100mm breathable plastic case (130mm x 138mm x 9.5mm) with ventilation holes to prepare a solid insect pest repellent.

[0083] Formulation Example 10 Slow-release living pest repellent: 3 g of an ethanol solution containing 1% of Product 1 of the present invention was placed in an open-topped plastic container (diameter 30 mm, depth 10 mm), and the opening was covered with a drug-permeable film to obtain a sustained-release repellent for living insect pests of clothing.

[0084] Formulation Example 11 Grain pest and daily life pest repellent A mixture of 0.01 g of Product 2 and 0.1 g of chili pepper powder (another pest repellent ingredient) was added to 15 g of ethanol, to which 10 g of hydroxypropylated guar gum dissolved in 24 g of ethanol was added, and after stirring, water was added to make 100 g, which was then filled into a container measuring 35 mm long x 35 mm wide x 75 mm high. The mixture was left to stand for 3 hours, yielding a gel-like grain pest repellent.

[0085] Formulation Example 12 Garbage can pest repellent The product 2 of the present invention was placed in a 5 ml round polyester container (opening area 8 cm 2 ) and the opening was closed with polyethylene film to obtain a flying insect repellent.

[0086] Usage example 1 When two tablets of the room temperature volatile insect repellent obtained in Formulation Example 2 were placed in a polyethylene clothing storage bag (75cm x 80cm x 32cm) along with clothing, the clothing remained free of mites and clothing pests for approximately one month.

[0087] Usage example 2 When two tablets of the room temperature volatile insect repellent obtained in Formulation Example 9 were placed in a polyethylene futon storage bag (150cm x 100cm) along with bedding, the bedding remained free of mites and clothing pests for approximately six months.

[0088] Usage example 3 When two tablets of the room temperature volatile insect repellent obtained in Formulation Example 2 were placed in a 75L (40cm x 75cm x 25cm) clothing case along with clothing, the clothing remained free of mites and other clothing pests for approximately three months.

[0089] Usage example 4 When the spray repellent for household pests obtained in Formulation Example 5 was sprayed evenly on clothes, no mites or clothing pests were found on the clothes for about one week.

[0090] Usage example 5 When the aerosol pest repellent obtained in Formulation Example 3 was sprayed evenly on a carpet, no mites were found on the carpet for about one month.

[0091] Usage example 6 When the pest repellent obtained in Formulation Example 11 was placed in a 30-liter polypropylene food storage container (355 mm x 472 mm x 253 mm), no grain pests invaded the container for about one year.

[0092] Usage example 7 When the pest repellent obtained in Formulation Example 12 was placed in an approximately 1.8 liter ABS trash can with a lid (160 mm x 80 mm x 155 mm) containing food waste, no fruit flies emerged for approximately one month. [Industrial Applicability]

[0093] The pest repellent of the present invention has an excellent insect repellent effect against pests and is safe for humans, so that it can be easily used in ordinary households and is highly useful. [Explanation of symbols]

[0094] 1. Microwave distillation apparatus 2 … … Distillation tank 3. Microwave heating device 4 … … Stirring splashes 5 ... Air flow inlet pipe 6 ... Distillate outflow pipe 7 … … Cooling device 8 ... Heating control device 9 ... ... Pressure reducing pump 10 ... ... Pressure regulating valve 11 ... ... Pressure control device 12 … … Object to be distilled 13 … … distillate 20 … … large petri dish 21 ... ... mite culture medium 22 ... ... small petri dish 23 … … Test piece 24 … … plastic containers 25 … … saturated salt water 30 ... ... Adult insect repellent test device 31 … … Test area 32 … … Control area 33 ... Passage pipe 34 … … Petri dish 35 … … Stainless steel basket 36 … … Air vent 37 … … Lid

Claims

1. A distillate obtained by heating and distilling plants of the genus Bidens (Asteraceae) under reduced pressure. A repellent for textile pests or grain pests, characterized in that the aqueous fraction is an active ingredient.

2. The Asteraceae plant of the genus Bidens is heated under reduced or normal pressure, and the temperature is determined by the water content of the plant alone. A fiber characterized in that the aqueous fraction of the distillate obtained by distilling the fiber is used as an active ingredient. A repellent for field or grain pests.

3. Bidens pilosa var. radiata Sc 3. The repellent for textile pests or grain pests according to claim 1 or 2, wherein the repellent is a repellent of a type selected from the group consisting of peanuts, ...

4. One or two species selected from the group consisting of flowers, leaves, stems, and roots of plants of the genus Bidens in the Asteraceae family The method for treating a textile pest or insect pest according to any one of claims 1 to 3, wherein the above-mentioned parts are subjected to a distillation treatment. or grain pest repellent.

5. A method for releasing the repellent for textile pests or grain pests according to any one of claims 1 to 4 into the environment. A method for repelling textile pests or grain pests, comprising:

6. The method for preventing and treating fiber pests or grain pests according to claim 5, wherein the release into the environment is carried out by natural evaporation at room temperature. Insect repellent methods.

7. Release into the environment is carried out by evaporation through heating, ventilation or fumigation. The method for repelling textile pests or grain pests according to claim 5.

8. 6. The method for repelling textile pests or grain pests according to claim 5, wherein the release into the environment is carried out by spraying.

9. The repellent for fiber pests or grain pests according to any one of claims 1 to 4 is applied to bedding or carpets. A method for repelling textile pests or grain pests, characterized in that the method is applied to

10. A repellent for textile pests or grain pests according to any one of claims 1 to 4 is applied to the inside of a storage container. A method for repelling textile pests or grain pests, comprising:

11. The repellent for textile pests or grain pests according to any one of claims 1 to 4 is applied to a garbage can or a garbage dump. A method for repelling textile pests or grain pests, characterized by applying the method to a gathering place.

12. A method for preventing insect pests from pesting textiles or grains, comprising applying the repellent according to any one of claims 1 to 4 to textile products. A method for repelling textile pests or grain pests, comprising:

13. The repellent for textile pests or grain pests according to any one of claims 1 to 4 is applied to textile pests or grain pests. A method for repelling textile pests or grain pests, which comprises contacting the pests with the pests to act on them.

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