Oligonucleotides and methods for site-specific editing of target RNA

JP7755837B2Active Publication Date: 2025-10-17FUKUOKA UNIV
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Patent Information

Application Number
JP2021037576
Authority / Receiving Office
JP · JP
Patent Type
Patents
Current Assignee / Owner
Priority Date
2020-03-12
Filing Date
2021-03-09
Publication Date
2025-10-17
Estimated Expiration
2041-03-09

AI Technical Summary

Technical Problem

Existing oligonucleotide constructs and RNA editing methods, such as those described in Patent Documents 1 and 2, lack the capability for efficient, site-specific editing of target RNA, particularly in a transient and non-permanent manner, as they rely on stem-loop structures that may not provide sufficient specificity and stability.

Method used

A target editing guide oligonucleotide is designed with a first oligonucleotide that identifies the target RNA, a second oligonucleotide linked to its 3' side, a third oligonucleotide capable of forming a complementary strand, and a first linking group connecting the 5' and 3' ends, enhancing site-specific editing by recruiting adenosine deaminase (ADAR) to convert adenosine residues to inosine, thereby altering RNA information temporarily.

Benefits of technology

The proposed oligonucleotide achieves site-specific editing of target RNA, offering improved specificity and stability, enabling temporary genetic modifications without permanent changes to the genome, and is applicable for treating genetic diseases and controlling protein function.

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Patent Text Reader

Abstract

To provide a target editing guide oligonucleotide capable of inducing site-specific editing.SOLUTION: A target editing guide oligonucleotide including: a first oligonucleotide which specifies a target RNA; a second oligonucleotide which is connected to 3' side of the first oligonucleotide; a third oligonucleotide having a base sequence which can form a complementary strand with the second oligonucleotide; and a first linking group which connects 5' terminal of the first oligonucleotide and 3' terminal of the third oligonucleotide. The first oligonucleotide consists of: a target-corresponding nucleotide residue which corresponds to an adenosine residue in the target RNA; 10 residues or more and 24 residues or less of a nucleotide chain connected to 5' side of the target-corresponding nucleotide residue, and has a base sequence complementary with the target RNA; and 2 residues or more and 7 residues or less of a nucleotide chain connected to 3' side of the target-corresponding nucleotide residue, and has a base sequence complementary with the target RNA. The target editing guide oligonucleotide induces site-specific editing to the target RNA.SELECTED DRAWING: Figure 1
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Citation Information

Patent Citations

  • Targeted RNA Editing

    JP2017537618A

  • RNA-guided nucleic acid modifying enzyme and method for using the same

    JP2019532644A

  • Methods and substances for directed RNA editing

    US20190093098A1

  • Targeted RNA editing

    WO2016097212A1

  • Method for inducing site-specific RNA mutations, target editing guide RNA used in method, and target RNA–target editing guide RNA complex

    WO2017010556A1