A method for growing real wasabi using oxygen nanobubble water

Abstract

To provide a plant raising method using oxygen nanobubble water which can promote germination and has extremely high germination rate.SOLUTION: A plant raising method using oxygen nanobubble water containing at least oxygen in nanobubbles, in which oxygen nanobubble water containing potassium ion is supplied to plant (Japanese horseradish) seeds in a germination season thereof to raise the plant. Preferably, oxygen nanobubble water containing all of three types of specific ion which are sodium ion, potassium ion and chloride ion is supplied to the plant (Japanese horseradish) seeds in the germination season thereof to raise the plant.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention relates to a plant cultivation method using oxygen nanobubble water, and more particularly to a seed germination method using oxygen nanobubble water containing ions of specific elements. [Background technology]

[0002] It has been known that growing plants using oxygen microbubble water or oxygen nanobubble water promotes plant growth, particularly during germination or immediately after germination, as the plants are able to take in more dissolved oxygen into their cells, accelerating the seedling growth rate and shortening the seedling raising period.

[0003] For example, Patent Document 1 discloses a hydroponic seedling raising method for raising agricultural crop seedlings, in which a hydroponic solution containing micro-nano bubbles is used during the seedling raising stage (see Claim 1 of Patent Document 1, paragraphs

[0026] to

[0035] of the specification, etc.).

[0004] However, although the hydroponic seedling raising method described in Patent Document 1 is said to result in a fast plant growth rate and an increased plant yield, there is a demand for even higher germination rates and faster growth rates.

[0005] Furthermore, Patent Document 2 discloses a hydroponic cultivation method for cultivating bonsai plants using a culture solution containing oxygen-containing micro-nano bubbles (see Claim 1 of Patent Document 2, paragraphs

[0012] to

[0023] of the specification, etc.).

[0006] Furthermore, Patent Document 2 gives examples of liquid fertilizers containing elements such as nitrogen (N), phosphorus (P), potassium (K), sulfur (S), calcium (Ca), magnesium (Mg), iron (Fe), manganese (Mn), zinc (Zn), copper (Cu), molybdenum (Mo), boron (B), chlorine (Cl), and nickel (Ni), which are taken up by bonsai plants from the outside through their roots (see paragraph

[0019] of the specification of Patent Document 2, etc.).

[0007] However, although the hydroponic cultivation method described in Patent Document 2 is said to be able to maintain the vitality of bonsai plants and to enable reliable cultivation of bonsai plants without the use of soil, it is limited to hydroponic cultivation of bonsai, and it was unclear whether it could also promote germination in seed plant cultivation. Promoting germination in seed plant cultivation has many benefits, such as improving seed utilization rate and reducing germination costs, and there is a strong demand for a plant cultivation method with an extremely high germination rate. [Prior art documents] [Patent documents]

[0008] [Patent Document 1] Japanese Patent Application Laid-Open No. 2015-97515 [Patent Document 2] International Publication No. 2019 / 087718 Summary of the Invention [Problem to be solved by the invention]

[0009] Therefore, the present invention has been devised in view of the above-mentioned problems, and its purpose is to provide a plant cultivation method using oxygen nanobubble water that can promote germination and has an extremely high germination rate. [Means for solving the problem]

[0010] The oxygen nanobubble water according to claim 1 is used. Genuine wasabi The cultivation method uses oxygen nanobubble water containing at least oxygen in the nanobubbles. The germination of real wasabi is promoted by A cultivation method comprising: Wasabi seeds The oxygen nanobubble water containing potassium ions is supplied during the germination period. Increase germination rate It is characterized by:

[0011] The oxygen nanobubble water according to claim 2 is used. Genuine wasabi The cultivation method uses the oxygen nanobubble water according to claim 1. Genuine wasabi In terms of cultivation methods, WasabiThe method is characterized by cultivating seeds by supplying them with oxygen nanobubble water containing all three specific ions: sodium ions, potassium ions, and chloride ions, during the germination period. [Effects of the Invention]

[0013] Claim 1 or 2 According to the invention, In real wasabi seeds, A high germination rate can be achieved.

[0014] In particular, according to the invention of claim 2, there is an even higher probability Wasabi seeds can be germinated. [Brief explanation of the drawings]

[0016] [Figure 1] FIG. 1 is a schematic diagram showing a plant growing apparatus used in a plant growing method using oxygen nanobubble water according to an embodiment of the present invention. [Figure 2] FIG. 2 is a graph showing the time course of germination of real wasabi, illustrating the results of the first verification experiment on the germination-promoting effects of three types of specific ion aqueous solutions. [Figure 3] FIG. 3 is a graph showing the change in germination of real wasabi over time, illustrating the results of the second verification experiment on the germination-promoting effects of three types of specific ion aqueous solutions. [Figure 4] Figure 4 is a graph comparing the time course of germination of wasabi seeds when different aqueous solutions were supplied, showing the results of an experiment verifying the germination-promoting effects of three types of specific ion aqueous solutions. [Figure 5] FIG. 5 is a graph showing the change in potassium ion concentration and the change in potassium ion absorption rate due to differences in the aqueous solution supplied. [Figure 6] FIG. 6 is a graph showing the change in calcium ion concentration and the change in calcium ion absorption rate and release rate due to differences in the aqueous solution supplied. DETAILED DESCRIPTION OF THE INVENTION

[0017] Hereinafter, one embodiment of a plant cultivation method using oxygen nanobubble water according to the present invention will be described in detail with reference to the drawings.

[0018] <Plant cultivation method using oxygen nanobubble water> A plant cultivation method using oxygen nanobubble water according to an embodiment of the present invention will be described using Figure 1. In the plant cultivation method using oxygen nanobubble water according to this embodiment, a case in which wasabi seeds are grown and germinated in hydroponics will be described as an example. Figure 1 is a schematic diagram showing a plant cultivation device 1 used in the plant cultivation method using oxygen nanobubble water according to this embodiment.

[0019] In the plant cultivation method using oxygen nanobubble water according to this embodiment, water is supplied at a temperature of approximately 10°C to 22°C, which is suitable for growing wasabi. As shown in FIG. 1, oxygen nanobubble water, which is expected to have an activating effect, is generated by oxygen nanobubble water generating means 2, and this water is supplied by pressure pump P to wasabi seeds grown hydroponically.

[0020] In this embodiment, as shown in Figure 1, real wasabi seeds were grown by making a slit in a culture medium 4 made of urethane foam or the like and inserting it into the medium, and then being hydroponically grown by supplying circulating water containing oxygen nanobubbles to the slit. In this embodiment, the circulating aqueous solution was replaced with a new one every certain period of time (every 7 days in this embodiment). Of course, it goes without saying that the water that has passed through the real wasabi seeds may be drained directly without being circulated.

[0021] In this embodiment, oxygen nanobubble water is prepared by pressurizing gases such as oxygen or air using a micro-nano bubble generator to dissolve them in a liquid to a supersaturated state, then rapidly reducing the pressure to generate microbubbles and nanobubbles in the liquid. After the microbubbles are separated by floating, only the nanobubbles remain in the liquid. However, oxygen nanobubble water refers to water containing fine oxygen gas bubbles on the nanometer order (1 μm or less) in diameter, and may also be micro-nanobubble water containing fine oxygen gas bubbles on the micrometer order (1 to 100 μm) in addition to nanometer-order oxygen gas bubbles. Furthermore, oxygen nanobubble water refers to water containing oxygen as at least nanometer-sized fine bubbles, including either oxygen nanobubbles or air nanobubbles, or both.

[0022] Specific examples of the oxygen nanobubble water generation method using the oxygen nanobubble water generation means 2 include the "swirl flow method," which mixes oxygen gas with water and swirls it at high speed to create oxygen bubbles, the "pressure dissolution method," which applies pressure to oxygen gas, dissolves it in water, and then suddenly releases the pressure to create oxygen bubbles, the "micropore method," which applies pressure to oxygen gas and passes it through micropores such as an orifice to create oxygen bubbles, and the "ultrasonic method," which uses ultrasound to cause cavitation, expanding the oxygen gas in water to create oxygen bubbles. However, the generation method of the oxygen nanobubble water generation means is not particularly limited, and any method can be used as long as it can generate macro-nano bubble water containing fine oxygen gas on the micron order (1 to 100 μm).

[0023] By converting oxygen into tiny nanobubbles, the buoyancy is reduced and the bubbles can remain suspended in water for a long time while undergoing Brownian motion, making it easier for plant roots to absorb oxygen from circulating water. This has the advantage of making it harder for bacteria to grow in the water, making it easier for plants to grow, even in hydroponic cultivation.

[0024] Furthermore, in the plant cultivation method using oxygen nanobubble water according to this embodiment, as shown in FIG. 1, sodium ions (Na + ), potassium ions (K +), and chloride ions (Cl - An aqueous solution of these three specific ions is placed in tank 3, mixed with the oxygen nanobubble water generated by oxygen nanobubble water generating means 2 in tank 3, and then pumped by pump P and supplied to culture medium 4 into which real wasabi seeds have been inserted. The effects of these three specific ions will be described based on experimental data in the experiments described below.

[0025] The germination period of real wasabi seeds refers to a certain period of time during which real wasabi seeds can germinate, from approximately 0 to 70 days after water begins to be supplied to the seeds.

[0026] (Verification experiment of the germination-promoting effect of three types of specific ion solutions) Next, we will explain a verification experiment that was conducted to verify the germination-promoting effects of the three specific ions mentioned above, in which the change in germination rate of real wasabi seeds over time (days) was recorded.

[0027] When using the above-described plant growing device 1 and supplying only ultrapure water, which is water with high purity, three types of specific ions (Na + ,K + ,Cl - ), when supplying a solution containing potassium permanganate (KMnO4), which is a strong oxidizing agent, when supplying ozone nanobubble water, when supplying oxygen nanobubble water + 3 types of specific ions (Na + ,K + ,Cl - ) and five different supply methods were used, and the germination rate of the wasabi seeds was measured for 50 days (the day water supply began was counted as day 0). Just to be sure, this verification experiment was conducted twice. The results of the first verification experiment are shown in Figure 2, and the results of the second are shown in Figure 3.

[0028] As is clear from FIGS. 2 and 3, similar to the plant cultivation method using oxygen nanobubble water according to the embodiment of the present invention, three types of specific ions (Na + ,K + ,Cl -In both the first and second experiments, it can be seen that when oxygen nanobubble water containing ) was supplied, the germination rate was overwhelmingly higher than in the other cases.

[0029] (Verification experiment when replaced with other metal ions) Next, the three specific ions mentioned above (Na + ,K + ,Cl - ) were replaced with other metal ions, the three specific ions mentioned above (Na + ,K + ,Cl - We conducted a verification experiment to examine the effect of ) on the germination rate of real wasabi. The results of the experiment are shown in Figure 4.

[0030] Specifically, when using the above-mentioned plant growing device 1 and supplying only ultrapure water, the above-mentioned three types of specific ions (Na + ,K + ,Cl - When supplying an aqueous solution containing the three specific ions (Na + ,K + ,Cl - When oxygen nanobubble water containing ) was supplied, three types of ions (Na + ,Ca 2+ ,Cl - When supplying an aqueous solution containing ions containing three types of ions (Na + ,Ca 2+ ,Cl - When oxygen nanobubble water containing ) was supplied, the wasabi seeds were supplied in five different ways, and the germination rate was measured for 70 days by counting the germination rate.

[0031] As is clear from FIG. 4, similar to the plant cultivation method using oxygen nanobubble water according to the embodiment of the present invention described above, three types of specific ions (Na + ,K + ,Cl -When oxygen nanobubble water containing 1 type of metal ion was supplied, the germination rate after 70 days was 71%, while when 3 types of ions (Na + ,Ca 2+ ,Cl - When oxygen nanobubble water containing 3 types of specific ions (Na) was supplied, the germination rate was 29%. This value was lower than the germination rate of 33% when only ultrapure water was supplied. + ,K + ,Cl - It was confirmed that when oxygen nanobubble water containing ) was supplied, the germination rate was extremely high.

[0032] (Verification experiment of changes in potassium ion and calcium ion concentrations) Next, since the aforementioned verification experiment showed that potassium ions were the key point, we measured the changes in potassium ion and calcium ion concentrations in each aqueous solution every seven days, which is the aqueous solution replacement cycle.

[0033] Specifically, when using the above-mentioned plant growing device 1 and supplying only ultrapure water, when supplying a potassium permanganate (KMnO4) aqueous solution, three types of specific ions (Na + ,K + ,Cl - When oxygen nanobubble water containing ) was supplied, three types of ions (Na) that replaced one type of metal ion were + ,Ca 2+ ,Cl - When oxygen nanobubble water containing ions (Na + ,K + ,Cl - When supplying an aqueous solution containing three specific ions (Na + ,Ca 2+ ,Cl - When supplying an aqueous solution containing ozone nanobubble water, the potassium ion (K + ) and calcium ions (Ca 2+The change in concentration of ) was measured. The measurement results are shown in Figures 5 and 6.

[0034] As is clear from FIGS. 5 and 6, three specific ions (Na + ,K + ,Cl - When oxygen nanobubble water containing potassium ions (K + ) and calcium ions (Ca 2+ ) is released. In other words, three specific ions (Na + ,K + ,Cl - When oxygen nanobubble water containing potassium ions (K + ) is absorbed by the seeds of real wasabi, and calcium ions (Ca 2+ ) is thought to promote germination.

[0035] According to the plant cultivation method using oxygen nanobubble water according to the present embodiment described above, as described above, both the oxygen nanobubble water and the plant contain at least potassium ions (K + ), the seeds of the wasabi plant can be made to germinate with an extremely high probability. This reduces the cost of germination and increases the yield of wasabi.

[0036] In addition, as in the plant cultivation method using oxygen nanobubble water according to the present embodiment, three types of specific ions (Na + ,K + ,Cl - By supplying oxygen nanobubble water containing potassium ions (K ions), the seeds of this wasabi are germinated. + ) absorption and calcium ions (Ca 2+ ) and improve the germination rate of real wasabi.

[0037] (Verification experiment of germination rate depending on the concentration of aqueous solution containing specific ions) Next, the three specific ions mentioned above (Na + ,K + ,Cl- ) and various combinations of specific ions, as well as when all three types of specific ions were supplied, the nanobubbles supplied were combined with no gas, oxygen, air, and ozone, and the salt concentration of the total ions relative to the total weight was measured as shown in Table 1 below, and verification experiments were conducted to observe the germination rate after 50 days (the day water supply began was counted as day 0) of supplying the nanobubbles to real wasabi. The results are shown in Table 1 below.

[0038] [Table 1]

[0039] As is clear from Table 1, three types of specific ions (Na + ,K + ,Cl - When oxygen nanobubble water, air nanobubble water, and ozone nanobubble water were supplied, the germination rates of real wasabi after 50 days of germination were 45% to less than 55%, less than 35%, and less than 22%, respectively. + ,Ca 2+ ,Cl - When oxygen nanobubble water and air nanobubble water containing 0.001% to 3% of two types of ions (Na) were supplied, the germination rates were less than 22% and less than 19%, respectively. + ,Cl - ) alone (less than 10%). + ,K + ,Cl - It was confirmed that when oxygen nanobubble water containing at least oxygen in the nanobubbles containing ) was supplied, the germination rate was extremely high.

[0040] Although the plant cultivation method using oxygen nanobubble water according to the embodiment of the present invention has been described in detail above, the above-described and illustrated embodiments are merely specific embodiments for carrying out the present invention, and therefore the technical scope of the present invention should not be interpreted as being limited by these embodiments. [Explanation of symbols]

[0041] 1:Plant growing equipment 2: Means for generating oxygen nanobubble water 3: Tank 4: Culture medium P: Pump

Claims

1. A method for cultivating real wasabi that promotes the germination of real wasabi using oxygen nanobubble water containing at least oxygen in nanobubbles, The oxygen nanobubble water containing potassium ions is supplied during the germination period of the wasabi seeds to increase the germination rate. A method for growing real wasabi using oxygen nanobubble water characterized by the above.

2. Cultivating wasabi seeds by supplying them with oxygen nanobubble water containing all three specific ions, sodium ions, potassium ions, and chloride ions, during the germination period. A plant cultivation method using the oxygen nanobubble water according to claim 1.

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