virus inactivating agent

Abstract

In one aspect of the present disclosure, an virus inactivator is i) alkaline ion electrolyzed water that contains purified water and at least sodium, silicon, and chlorine as contained substances and that contains more than 99.5 weight% purified water, or ii) a dilution provided by diluting the alkaline ion electrolyzed water with purified water that is not more than two-fold on a volume basis that of the alkaline ion electrolyzed water.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This international application claims priority based on Japanese Patent Application No. 2020-208804, filed with the Japan Patent Office on December 16, 2020, the entire contents of which are incorporated herein by reference. [Technical Field]

[0002] The present disclosure relates to a virus inactivating agent that inactivates a virus. [Background technology]

[0003] Recently, a new coronavirus called SARS-CoV-2 has become widespread. To prevent viral infection, people are washing their hands with water and soap, and using alcohol-based disinfectants, as described in Non-Patent Document 1.

[0004] The present inventors have intensively searched for substances that can inactivate viruses more effectively. [Prior art documents] [Non-patent literature]

[0005] [Non-Patent Document 1] The National Institute of Technology and Evaluation(NITE) “Final Report on Efficacy Assessment of Disinfecting Substances Alternative to Alcohol for Use Against Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)” The Committee on Efficacy Assessment of Disinfecting Substances Alternative to Alcohol for Use Against Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2)June, 2020 Summary of the Invention [Problem to be solved by the invention]

[0006] One aspect of the present disclosure preferably provides a virus inactivating agent capable of inactivating the novel coronavirus.

[0007] Another aspect of the present disclosure is to preferably provide a virus inactivating agent that can inactivate the novel coronavirus and is also gentle on human skin. [Means for solving the problem]

[0008] A first aspect of the present disclosure is i) Alkaline ionized water containing purified water and at least sodium, silicon, and chlorine as components, and containing more than 99.5% by weight of the purified water; or ii) a diluted solution obtained by diluting the alkaline ionized water with purified water in an amount of not more than twice the volume of the alkaline ionized water; It is a virus inactivating agent capable of inactivating viruses.

[0009] A second aspect of the present disclosure is a virus inactivation agent capable of inactivating viruses, comprising alkaline ionized water, The alkaline ionized water is a virus inactivating agent containing at least sodium, silicon, and chlorine. [Brief explanation of the drawings]

[0010] [Figure 1] 1 is a photograph showing how a subject sample of a test sample is measured by a plaque measurement method in Example 1. [Figure 2] 1 is a photograph showing how a test sample is measured by a plaque measurement method in Example 1. DETAILED DESCRIPTION OF THE INVENTION

[0011] An exemplary embodiment of the present disclosure (hereinafter referred to as "the present embodiment") will be described.

[0012] The virus inactivating agent of this embodiment inactivates viruses, such as SARS-CoV-2 (novel coronavirus) that causes COVID-19 (novel coronavirus infection). "Inactivation" refers to killing a virus or eliminating its infectivity and toxicity.

[0013] The virus inactivating agent of this embodiment is either i) alkaline ionized water containing purified water and at least sodium, silicon, and chlorine as ingredients, and containing more than 99.5% by weight of purified water, or ii) a diluted solution obtained by diluting alkaline ionized water with purified water in an amount equal to or less than twice the volume of the alkaline ionized water. The content of purified water in the alkaline ionized water is preferably 99.7% by weight or more.

[0014] The virus inactivating agent of the present embodiment contains alkaline ionized water, and the alkaline ionized water contains at least sodium, silicon, and chlorine as its components.

[0015] Alkaline ionized water is generally said to have a beneficial effect on the living body. For example, alkaline ionized water is said to have the effect of improving gastrointestinal symptoms, and has been used for medicinal purposes. While the inventor was independently developing alkaline ionized water that is beneficial to the living body, he discovered that a certain special alkaline ionized water has the ability to inactivate viruses. This was far from the inventor's expectation that alkaline ionized water would improve the functioning of the living body. Therefore, the inventor came up with the idea of ​​using alkaline ionized water as a virus inactivating agent.

[0016] The virus inactivating agent of this embodiment has virus inactivation performance comparable to or superior to that of detergents, disinfectants, and ethanol. The virus inactivating agent of this embodiment exhibits excellent virus inactivation effects even without containing known virus infection inhibitors such as detergents, disinfectants, and ethanol.

[0017] The alkaline ionized water of this embodiment is characterized by containing at least sodium, silicon, and chlorine as ingredients. Due to this characteristic, the alkaline ionized water of this embodiment is able to exhibit an excellent virus inactivation effect, unlike other alkaline ionized waters.

[0018] Alkaline ionized water is alkaline water, for example, containing cations and OH - Alkaline ionized water is also called reducing ionized water, and can be said to be water with a high electron content. This type of alkaline ionized water has a high electron content and is said to promote normal functioning of the body.

[0019] The alkaline ionized water may be any alkaline water, and is preferably alkaline ionized water produced by electrolysis. The alkaline ionized water is obtained by electrolyzing base water containing electrolytes, and contains cations. The alkaline ionized water contains more cations than anions, and exhibits alkalinity.

[0020] The alkaline ionized water of this embodiment contains at least sodium, silicon, and chlorine as ingredients. In this embodiment, "ingredients" refers to solutes contained in the alkaline ionized water. When the water (H2O) in the alkaline ionized water is used as a solvent, the ingredients are contained in the alkaline ionized water as solutes. Most of the ingredients are inorganic and dissolved in water. By containing these ingredients, alkaline ionized water has a higher virus inactivation effect than water that does not contain these ingredients.

[0021] The alkaline ionized water of this embodiment may further contain at least one of phosphorus and potassium as an ingredient. The alkaline ionized water of this embodiment may also contain at least one of calcium and magnesium as an ingredient. Here, the ingredient may be contained in the virus inactivating agent in the form of a cation, anion, or hydroxide, or in the form of a salt. The ingredient may be produced by electrolysis of an electrolyte or by adding a salt to purified water.

[0022] The virus inactivating agent of this embodiment can inactivate viruses.

[0023] The virus inactivating agent of this embodiment has been confirmed to have low cytotoxicity, that is, to have little effect on human skin.

[0024] The pH of the virus inactivating agent of this embodiment is preferably 10 to 13, and more preferably 11.5 to 12.5 (12±0.5). For reasons unknown, this allows for effective inactivation of viruses while reducing the impact on human skin when the virus inactivating agent comes into contact with the human skin.

[0025] The alkaline ionized water contained in the virus inactivating agent should have a pH of 12±0.5. Of the alkaline ionized water, alkaline ionized electrolyzed water should be adjusted to a pH of 12.0±0.5. The reason for this is unclear, but it is because the pH of the diluted solution formed by diluting the alkaline ionized water is stable.

[0026] The virus inactivating agent includes purified water. Purified water refers to water whose purity has been increased by purification such as distillation, ion exchange, or filtration. Examples of purified water include distilled water, ion-exchanged water, filtered water, and pure water.

[0027] The virus inactivating agent may contain purified water in an amount of 98% by weight or more, preferably more than 99.5% by weight, and more preferably 99.7% by weight or more, and can effectively inactivate viruses.

[0028] The virus inactivating agent of this embodiment contains alkaline ionized water such as alkaline ionized water. When the virus inactivating agent of this embodiment is taken as 100% by weight, the total content of ingredients contained in the virus inactivating agent is preferably 0.001% by weight or more, more preferably 0.01% by weight or more, and particularly preferably 0.1% by weight or more. The total content of ingredients contained in the virus inactivating agent may be 2% by weight or less, and more preferably 0.5% by weight or less. In this case, viruses can be effectively inactivated.

[0029] If the total content of ingredients is too high, there is a risk that it may affect skin cells when a person touches the virus inactivating agent. The "total content of ingredients" refers to the total content of all ingredients contained in the virus inactivating agent of this embodiment, and specifically refers to the total content of all ingredients contained in the virus inactivating agent, such as sodium, silicon, chlorine, phosphorus, potassium, calcium, and magnesium.

[0030] When the virus inactivating agent is taken as 100% by weight, the sodium content may be 0.0001-1% by weight, or may be 0.0001-0.3% by weight, and more preferably 0.001-0.2% by weight, and more preferably 0.01-0.2% by weight. When the total of the ingredients in the virus inactivating agent is taken as 100% by weight, the sodium content is preferably 10-60% by weight, and more preferably 20-50% by weight. "Total of ingredients" refers to the sum of all ingredients contained in the virus inactivating agent. If the sodium content is too low, virus inactivation may be reduced, while if the sodium content is too high, it may have an effect on skin cells.

[0031] When the virus inactivating agent is taken as 100% by weight, the silicon content can be 0.00001-0.01% by weight, or alternatively, 0.00001-0.001% by weight, preferably 0.00005-0.005% by weight, and even more preferably 0.0001-0.005% by weight. When the total content of the virus inactivating agent is taken as 100% by weight, the silicon content is preferably 0.2-5% by weight, and even more preferably 0.5-0.9% by weight. In this case, the virus inactivation by the virus inactivating agent is further enhanced. If the silicon content is too low, virus inactivation may be reduced, while if the silicon content is too high, it may have an effect on skin cells.

[0032] When the virus inactivating agent is taken as 100% by weight, the chlorine content may be 0.0001-0.1% by weight, more preferably 0.001-0.05% by weight, and preferably 0.005-0.05% by weight. When the total content of the virus inactivating agent is taken as 100% by weight, the chlorine content is preferably 5-15% by weight, and more preferably 7-12% by weight. If the chlorine content is too low, there is a risk of reduced virus inactivation, while if the chlorine content is too high, there is a risk of adverse effects on skin cells.

[0033] When the virus inactivating agent is taken as 100% by weight, the potassium content may be 0-1% by weight, or may be 0-0.2% by weight, or more preferably 0.003-0.15% by weight, and preferably 0.01-0.15% by weight. When the total content of the virus inactivating agent is taken as 100% by weight, the potassium content is preferably 0-50% by weight, and more preferably 20-40% by weight. If the potassium content is too low, there is a risk of reduced virus inactivation, while if the potassium content is too high, there is a risk of adverse effects on skin cells.

[0034] When the virus inactivating agent is taken as 100% by weight, the phosphorus content may be 0-0.1% by weight, preferably 0.002-0.1% by weight, and even more preferably 0.01-0.1% by weight. When the total contents of the virus inactivating agent is taken as 100% by weight, the phosphorus content is preferably 0-40% by weight, and even more preferably 10-30% by weight. In this case, the virus inactivation effect of the virus inactivating agent is further enhanced. If the phosphorus content is too low, there is a risk of reduced virus inactivation, while if the phosphorus content is too high, there is a risk of adverse effects on skin cells.

[0035] When the virus inactivating agent is taken as 100% by weight, the calcium content may be 0-0.002% by weight, and preferably 0-0.0002% by weight. When the total content of the virus inactivating agent is taken as 100% by weight, the calcium content is preferably 0-20% by weight, and more preferably 0-2% by weight. In this case, virus inactivation by the virus inactivating agent is improved.

[0036] When the virus inactivating agent is taken as 100% by weight, the magnesium content may be 0-0.001% by weight, and preferably 0-0.0001% by weight. When the total content of the virus inactivating agent is taken as 100% by weight, the magnesium content is preferably 0-10% by weight, and more preferably 0-1% by weight. In this case, virus inactivation by the virus inactivating agent is improved.

[0037] The composition of the virus inactivating agent of this embodiment preferably contains any of the following combinations when the virus inactivating agent is taken as 100% by weight.

[0038] i) 0.0001-1 wt% sodium, 0.00001-0.01 wt% silicon, 0.0001-0.1 wt% chlorine, 0-1 wt% potassium, and 0-0.1 wt% phosphorus; ii) 0.0001-0.3 wt% sodium, 0.00001-0.001 wt% silicon, 0.0001-0.1 wt% chlorine, 0-0.2 wt% potassium, and 0-0.1 wt% phosphorus; iii) 0.001-0.2 wt% sodium, 0.00005-0.005 wt% silicon, 0.001-0.05 wt% chlorine, 0.003-0.15 wt% potassium, and 0.002-0.1 wt% phosphorus; or iv) 0.01-0.2 wt% sodium, 0.0001-0.005 wt% silicon, 0.005-0.05 wt% chlorine, 0.01-0.15 wt% potassium, and 0.01-0.1 wt% phosphorus.

[0039] The composition of the virus inactivating agent of this embodiment is as follows, when the total amount of the components contained in the virus inactivating agent is 100% by weight: v) 10-60% by weight sodium, 0.2-5% by weight silicon, 5-15% by weight chlorine, 0-50% by weight potassium, and 0-40% by weight phosphorus; or vi) 20-50% by weight sodium, 0.5-0.9% by weight silicon, 7-12% by weight chlorine, 20-40% by weight potassium, and 10-30% by weight phosphorus It is good to include:

[0040] In the virus inactivating agent of this embodiment, the ratio of the total weight of sodium and potassium to the total weight of phosphorus, silicon, and chlorine is preferably 1-3, more preferably 1.5-2.5. Phosphorus, silicon, and chlorine may optionally be present together with oxygen as a main anion (e.g., PO4 3― , SiO4 4- , Cl - , ClO -), and sodium and potassium form cations (Na + , K. + ) are elements that form the virus inactivating agent. When the ratio of the total weight of sodium and potassium to the total weight of phosphorus, silicon, and chlorine is greater than 1, the virus inactivating agent can exhibit alkalinity suitable for inactivating viruses. When the ratio of the total weight of sodium and potassium to the total weight of phosphorus, silicon, and chlorine is excessively large, the alkalinity is too strong, and there is a risk that the virus inactivating agent will affect the skin when it comes into contact with it.

[0041] In the virus inactivating agent of this embodiment, the weight ratio of phosphorus to silicon is preferably 10-50, and more preferably 20-40, in order to enhance virus inactivation.

[0042] The components and amounts of the ingredients contained in the virus inactivating agent of this embodiment can be identified by, for example, atomic absorption spectrometry, ICP atomic emission spectrometry, or combustion-coulometric titration.

[0043] The virus inactivating agent of this embodiment is not particularly limited as long as it has the above-mentioned composition. For example, iW-100 and S-100 manufactured by AI System Products, Inc. can be used. iW-100 is alkaline ionized water with a pH of 11.5-12.5, containing sodium, silicon, chlorine, phosphorus, and potassium, with a total content of these elements of 0.5 wt% or less, and a water content of 99.5 wt% or more. S-100 is alkaline ionized water with a pH of 11.5-12.5, containing silicon, sodium, chlorine, calcium, potassium, phosphorus, and magnesium, with a total content of these elements of 1.2 wt%, and a water content of 99.7 wt%. The commercially available S-100 was able to inactivate the SARS-CoV-2 virus and influenza virus. Furthermore, iW-100 was able to inactivate the SARS-CoV-2 virus, as shown in the experimental examples described below.

[0044] The method for producing a virus inactivating agent of the present embodiment includes a step of producing alkaline ionized water containing at least sodium, silicon, and chlorine, and optionally further containing at least one of potassium, phosphorus, calcium, and magnesium.

[0045] Furthermore, the method for producing the virus inactivating agent of this embodiment preferably includes a step of obtaining alkaline ionized water by electrolyzing raw water containing at least sodium, silicon, and chlorine, and optionally further containing at least one of potassium, phosphorus, calcium, and magnesium, in an electrolytic cell. The alkaline ionized water is preferably recovered from near the cathode after electrolysis in an electrolytic cell equipped with a cathode and an anode.

[0046] The original water is produced by adding ingredients to the purified water, which may be electrolytes or ingredients derived from seawater, or / and ingredients dissolved from soil or rocks.

[0047] An electrolyte refers to a substance that can be ionized into cations and anions by electrolysis, and examples of such substances include salts. Salts that can serve as electrolytes preferably include at least sodium chloride, and may further include, but are not limited to, potassium chloride, sodium carbonate, and potassium carbonate. Various other salts, such as silicates such as sodium silicate and phosphates such as sodium phosphate, may also be used as electrolytes.

[0048] Seawater is primarily composed of water, with approximately 3.5% by weight of salt and trace metals. Seawater contains salts such as sodium chloride, magnesium chloride, magnesium sulfate, calcium sulfate, and potassium chloride. Soil and rock contain a lot of silicon. Depending on the type, soil and rock contain calcium, potassium, sodium, magnesium, and phosphorus in addition to silicon. The type of soil and rock to be used should be selected so that it matches the composition of the alkaline ionized water to be produced.

[0049] To electrolyze the raw water, it is preferable to apply an electric energy of 100 to 300 W to the raw water. Electrolysis is preferably carried out in a sealed electrolytic cell. The electrolytic cell is preferably in an inert atmosphere such as nitrogen or carbon dioxide. Alternatively, an oxygen remover may be added to the electrolytic cell. The oxygen remover may be any material that has the property of contributing to the removal of oxygen from the electrolytic cell, such as Ageless (registered trademark, Mitsubishi Gas Chemical Company, Inc.).

[0050] Alkaline ionized water may be used as a virus inactivating agent as a stock solution, or it may be diluted before use. In this specification, alkaline ionized water itself is also referred to as a stock solution. Alkaline ionized water may be diluted by adding purified water. When the ratio of the weight of the virus inactivating agent to the weight of the alkaline ionized water is taken as the dilution factor of the alkaline ionized water, the dilution factor of the alkaline ionized water is preferably 10 times or less, more preferably 5 times or less, and desirably 3 times or less. A virus inactivating agent whose dilution factor with alkaline ionized water is within this range can exhibit high virus inactivation performance.

[0051] The virus inactivating agent of the present embodiment may further contain additives, such as colorants, fragrances, stabilizers, moisturizers, preservatives, surfactants, and ethanol.

[0052] Another aspect of this embodiment is a virus inactivating agent having alkaline ionized water containing at least sodium, silicon, and chlorine as ingredients.

[0053] Another aspect of this embodiment is the use of a solution having alkaline ionized water for virus inactivation, wherein the alkaline ionized water contains at least sodium, silicon, and chlorine.

[0054] Another aspect of this embodiment is a method of inactivating viruses using a solution having alkaline ionized water, the alkaline ionized water having at least sodium, silicon, and chlorine as ingredients.

[0055] Another aspect of this embodiment is use of alkaline ionized water for producing a virus inactivating agent, wherein the alkaline ionized water contains at least sodium, silicon, and chlorine.

[0056] The virus inactivating agent of this embodiment can be used to inactivate viruses, and can be suitably used, for example, to inactivate SARS-CoV-2 (novel coronavirus) that causes COVID-19 (novel coronavirus infection).

[0057] The virus inactivating agent of this embodiment can be used to inactivate not only SARS-CoV-2 (novel coronavirus) but also influenza viruses and most viruses.

[0058] The virus inactivating agent of this embodiment may be a virus inactivation liquid. The virus inactivating agent of this embodiment may be, for example, a liquid like a virus inactivation liquid, a semi-liquid such as a gel, or a solid. A gel-like virus inactivating agent may further contain a thickener, and a solid virus inactivating agent may further contain a gelling agent. The thickener is not particularly limited as long as it makes the virus inactivating agent semi-liquid, and for example, a polysaccharide may be used. Examples of polysaccharides that can be used as thickeners include carboxymethylcellulose, dextrin, pectin, guar gum, xanthan gum, tamarind gum, carrageenan, and propylene glycol. The gelling agent is not particularly limited as long as it makes the virus inactivating agent solid, and examples include agar, silica gel, and gelatin.

[0059] The virus inactivating agent of the present embodiment may be used alone or may be mixed with a product, such as laundry detergent, disinfectant, kitchen detergent, ethanol, cosmetics, nasal drops, nasal wash, or mouthwash.

[0060] When using the virus inactivating agent of this embodiment, it is advisable to bring at least a part of a human body (e.g., a hand or a finger) that is at risk of virus infection into contact with the virus inactivating agent. The virus inactivating agent may be applied or sprayed onto the surface of an object that may come into contact with the human body, or may be sprayed in a human living space.

[0061] When applying the virus inactivating agent of this embodiment, for example, the virus inactivating solution, the amount of application is 0.005 to 5.0 ml / 1 cm. 2 Furthermore, 0.01-1.0ml / 1cm 2 is preferred, and 0.1 ml / 1 cm 2 The amount of the virus inactivating solution to be sprayed in this embodiment is preferably 15-6000 ml / 16 m. 3 Furthermore, 30-3000ml / 16m 3 is preferred, 300ml / 16m 3 A degree of this is desirable.

[0062] The time of contact with the virus inactivating agent is preferably 2 seconds or more, more preferably 10 seconds or more, but may be 2 minutes or less.

[0063] Examples of objects that may come into contact with the human body include textile products such as clothing, hats, towels, masks, bedding, sofas, wet towels, and cushions; decorative items such as rings, necklaces, earrings, piercings, and hair clips; residential components such as floors, walls, windows, and doors; household products such as kitchen counters, toilet seats, doorknobs, bathtubs, faucets, washbasins, home appliances, and kitchen utensils; furniture such as desks, chairs, shelves, and counters; electronic devices such as multifunction printers, personal computers, and keyboards; public equipment such as ticket machines, vending machines, automatic teller machines, automatic ticket gates, straps, equipment control panels, shopping carts, and handrails, as well as general nursing care equipment and general medical equipment. [Example]

[0064] Example 1 1.Virus inactivation solution In the manufacturing process of the virus inactivating solution of this example, purified water is passed through a filter layer that appropriately contains ingredients such as sodium, silicon, chlorine, phosphorus, and potassium to produce raw water containing these ingredients.The raw water is then electrolyzed with a target pH of 12 to produce a stock solution consisting of alkaline ionized water.

[0065] The filter layer can be made of any material (earth, sand, rocks, or other materials that can be used to form a filter layer) as long as the filtered alkaline ionized water contains at least 99.5% by weight of purified water and 0.5% by weight of other substances after filtration and electrolysis. The filter layer is made by mixing the main components and adjusting the size so that the filtered raw water contains at least sodium, silicon, and chlorine.

[0066] The ingredients used are rocks containing phosphorus and silicon, and seawater containing sodium, chlorine, and potassium. This makes the virus inactivating liquid gentle on the human body and the natural environment.

[0067] The ratio of the above-mentioned substances (sodium, silicon, chlorine, phosphorus, and potassium) to purified water varies depending on the filtration conditions, as does the ratio of the substances to the original water. The pH also varies depending on the amount and ratio of the substances to the purified water.

[0068] Based on the inventor's decades of experience, he has now discovered that a concentrate consisting of alkaline ionized water produced by electrolyzing raw water containing more than 99.5% purified water by weight and containing at least sodium, silicon, and chlorine at a target pH of 12.0 is natural and gentle on the skin, and has applied it to himself and his collaborators.

[0069] The following experiment has now discovered that the above-mentioned concentrate has antiviral effects, particularly against the SARS-CoV-2 virus (a new use).

[0070] Based on this discovery, we conducted the following experiment to prove that the above-mentioned stock solution can be used as an inactivation solution for the SARS-CoV-2 virus. The following experiment was conducted using a standard method for verifying the performance of virus inactivation. The method for producing the stock solution and the experimental method are described below. <How to make the stock solution> Purified water was passed through a filter layer containing appropriate amounts of sodium, silicon, chlorine, phosphorus, potassium, and other elements to produce raw water containing these elements within the purified water. The filter layer was a tank (60 cm inner diameter) filled to a height of 10 cm with rock crushed to an average diameter of 0.1 mm. The rock type was igneous rock. 80 L of raw water and 80 L of seawater were introduced into a filter tank (tank) equipped with a filter layer filled with rock, and the mixture was stored for 24 hours with appropriate stirring. The composition of the introduced seawater was 30 wt% sodium, 55 wt% chlorine, 1 wt% potassium, and the remainder trace elements, assuming the total of all solutes contained in the seawater to be 100 wt%.

[0071] The raw water was removed from the filtration layer and subjected to electrolysis. To perform the electrolysis, a cathode and an anode were placed in the cathode chamber and the anode chamber, which were separated by a diaphragm, respectively. The raw water was introduced into the electrolysis cell. The electrolysis conditions were a voltage of 200 V and an electric energy of 200 W. After electrolysis, the stock solution consisting of alkaline ionized water present near the cathode was recovered. The pH of the stock solution consisting of alkaline ionized water was 12.0. <Test Overview> 1: Test location: Kobe Testing Center, Japan Textile Products Quality Technology Center, General Incorporated Foundation 2: Test virus: NIID isolate provided by the National Institute of Infectious Diseases: JPN / TY / WK-521 (SARS-CoV-2 virus) 3: Host cell: VeroE6 / TMPRSS2 4: Cell culture medium: EMEM (Minimum Essential Medium Eagle) DMEM(Dulbecco's modified Eagle's medium)(low-glucise) 5: Fetal Bovine Serum (FBS) 6: Target sample (Negative control): PBS (Phosphate buffered saline) 7: Test sample (this example): 1) Virus inactivation solution (undiluted solution) 2) Virus inactivation solution (2x diluted solution) :3) Virus inactivation solution (3x diluted solution) *The original solution is iW-100 (alkaline ionized water), which is diluted with purified water. The dilution ratio is the weight ratio of the virus inactivation solution to the original solution. 8: Drug inactivating agent: SCDLP (Soybean-Casein Digest Broth with Lecithin & Polysorbate 80) was diluted 10-fold with DMEM containing 2% by weight of FBS to be used as a drug inactivating agent. 9: Infectivity titer measurement method: Plaque measurement method (JIS L 1922) 10: Virus suspension: EMEM was added to host cells infected with SARS-CoV-2 virus, and the cells were cultured at 37°C for a predetermined time. The supernatant was centrifuged at 1000 × g for 15 minutes at 4°C to obtain the virus suspension for testing. The virus concentration in the virus suspension was 2.5 × 10 8 It was expressed as PFU / ml. <Test content and test results> [Test 1: Cytotoxicity confirmation test] <Test Contents> (Test to confirm whether the test sample is toxic or not) 1. 0.1 mL of EMEM and 1.9 mL of the test sample were thoroughly mixed to prepare a toxicity test solution.

[0072] 2. 0.1 mL of the toxicity test solution was added to 0.9 mL of the drug inactivator and stirred thoroughly.

[0073] 10 DMEM containing 3.2% by weight of FBS was used. 0 double, 10 1 double, 10 2 double, 10 3 double, 10 4 A 2-fold dilution series was prepared.

[0074] 4. The presence or absence of cytotoxicity in each dilution series was confirmed using the plaque assay method. Test Results: Cytotoxicity confirmation tests are essential safety tests for medical devices that come into direct contact with body tissues and blood. Table 1 shows the results of the cytotoxicity confirmation test. In Table 1, if no toxicity was found in the cytotoxicity confirmation test, the result is marked as "None." The test samples of this example were not toxic, even in their undiluted form.

[0075] [Table 1] [Test 2: Test to confirm cell susceptibility to viruses] (Test to confirm whether or not the drug inactivator has the reaction-stopping function) <<Exam Contents>> 1. 0.1 mL of EMEM and 1.9 mL of the test sample were thoroughly mixed to prepare a test solution.

[0076] 2. 0.5 mL of the test solution was added to 4.5 mL of the drug inactivator and stirred thoroughly.

[0077] 10 DMEM containing 3.2% by weight of FBS was used. 0 double, 10 1 double, 10 2 double, 10 3 double, 10 4 A 2-fold dilution series was prepared.

[0078] 4. 4.3 x 10 using EMEM 4 The above-mentioned virus suspension adjusted to PFU / ml was added to each dilution series in a volume of 1 / 100 of the dilution series.

[0079] 5. Each dilution series from step 4 above was allowed to stand at room temperature for 10 minutes.

[0080] 6. The virus infectivity per 1 mL of each dilution series was measured using the plaque assay method, and the susceptibility of the cells to the virus was confirmed. Test Results Table 1 shows the results of a test to confirm the susceptibility of cells to viruses. Susceptibility was determined by calculating the common logarithm mean of the viral infectivity titer, and based on this common logarithm mean, determining whether or not the condition of the calculation formula · Ig (viral infectivity titer of PBS (PFU / mL)) - Ig (viral infectivity titer of test sample (PFU / mL)) ≦ 0.5 was met. Meeting the conditions of this calculation formula indicates that viral growth has stopped, and failure to meet the conditions indicates that the virus has been inactivated.

[0081] In this example, the virus infectivity titers of the undiluted solution, 2x diluted solution, and 3x diluted solution all differed by 0.5 or less from the virus infectivity titer of PBS, confirming that the drug inactivator stopped the proliferation of the SARS-CoV-2 virus. [Test 3: Main Test] (Test to confirm whether or not the virus is inactivated) <<Exam Contents>> 1. 0.1 mL of virus suspension was mixed with 1.9 mL of the target sample or test sample, and the mixture was left to stand at 25°C for 20 seconds, 1 minute, and 5 minutes to prepare the infection test liquid.

[0082] 2. 0.1 mL of the infection test solution was added to 0.9 mL of the drug inactivator and thoroughly stirred to stop the growth of the virus in the infection test solution. This was used as the reaction stop solution.

[0083] 3. Add 10 ml of the reaction stop solution from step 2 above. 0 10 in DMEM containing 2% FBS by weight. 0 double, 10 1 double, 10 2 double, 10 3 double, 10 4 For each dilution series, the virus infectivity per 0.1 ml of the infection test solution was measured by plaque assay, and the virus infectivity per 1 ml of the infection test solution was calculated.

[0084] 4. The results of the test to confirm whether or not the virus was inactivated are shown in Table 2. In Table 2, n1, n2, and n3 indicate that the test was conducted three times for each target sample or test sample.

[0085] [Table 2] Test Results As shown in Table 2, for the control sample (PBS), the common logarithm of the virus infectivity titer remained nearly constant. This indicates that the drug inactivator was functioning effectively. In contrast, for the test samples, the undiluted iW-100 (alkaline ionized water) inactivated over 99.99% of the virus 20 seconds after mixing with the virus suspension. The 2x diluted solution inactivated over 99.99% of the virus after 1 minute. Even the 3x diluted solution inactivated nearly 99.99% of the virus after 1 minute. This demonstrates that not only the undiluted alkaline ionized water, but also diluted solutions obtained by diluting alkaline ionized water with purified water at a volume less than twice the volume of alkaline ionized water, have excellent virus inactivation performance. <Measurement using the plaque measurement method> Figure 1 is a photograph taken of the state of the test virus in a petri dish for Test 2. Figure 2 is a photograph taken of the state of the test virus in a petri dish for Test 3.

[0086] As shown in Figure 1, when the stop solution is not diluted with PBS (dilution series 10 0 10000 times (dilution series 10) the number of plaques was too large to count. 4 The number of countable plaques was 100 times higher than that in the control group. Thus, the proliferation of the virus was remarkable in the control group.

[0087] On the other hand, as shown in FIG. 2, the iW-100 (alkaline ionized water) of this example, not only the original solution but also the 3-fold diluted solution, showed no significant difference in the reaction rate when not diluted with the reaction stop solution (10 0 Even at a concentration of 100 ppb (2x), it was possible to count the number of plaques by the plaque measurement method. This demonstrates that iW-100 in this example inactivates viruses.

[0088] As explained above, iW-100 of this example exhibits no cytotoxicity even in its undiluted form, meaning that no effects on the human body were confirmed. Even in a 3-fold diluted solution, nearly 99.99% of the virus was inactivated within 1 minute. This demonstrates that the virus inactivation solution of this example can inactivate SARS-CoV-2 to a level where the antiviral activity value increases by 3 or more in the common logarithm mean of the viral infectivity titer within 1 minute. It was also found that a diluted solution obtained by diluting the iW-100 undiluted solution of this example to a volume no more than twice the original volume can inactivate the novel coronavirus and is gentle on human skin.

[0089] Therefore, by spraying the iW-100 of this embodiment onto human skin, the novel coronavirus can be inactivated while reducing the impact on human skin. Example 2 <Composition of virus inactivation solution> iW-100 (a stock solution consisting of alkaline ionized water) was produced using the same method as described in Example 1 for the production of the stock solution under "Production Method of Stock Solution." The sodium (Na), phosphorus (P), potassium (K), silicon (Si), and chlorine (Cl) contained in the stock solution were measured. Measurements were conducted by the Japan Food Research Center. The sodium and potassium contained in iW-100 were measured by atomic absorption spectrometry, phosphorus and silicon by ICP atomic emission spectrometry, and chlorine by combustion-coulometric titration. The weight ratio of phosphorus to silicon in the stock solution was also determined, as was the ratio of the total weight of sodium and potassium to the total weight of phosphorus, silicon, and chlorine. While trace components may be present in the contents, the total weight of the contents was calculated as the sum of the weights of sodium, phosphorus, potassium, silicon, and chlorine. The number of iW-100 samples (n) was five. The measurement results are shown in Table 3.

[0090] Furthermore, the inventors measured the pH of each iW-100 and found that all were 12.0.

[0091] [Table 3] Three types of virus inactivation solutions were prepared from the five iW-100 solutions shown in Table 3: undiluted solution, 2-fold diluted solution, and 3-fold diluted solution. The average values ​​of each component and their total content in these virus inactivation solutions are shown in Table 4. All three types of virus inactivation solutions prepared (undiluted solution, 2-fold diluted solution, and 3-fold diluted solution) had a pH of 12.0.

[0092] [Table 4] a) From the above results, when the virus inactivation solution is taken as 100% by weight, 0.0001-1 wt% sodium, 0.00001-0.01 wt% silicon, 0.0001-0.1 wt% chlorine, 0-1 wt% potassium, and 0-0.1 wt% phosphorus, When the sodium content is 0.0001-0.3 wt%, silicon content is 0.00001-0.001 wt%, chlorine content is 0.0001-0.1 wt%, potassium content is 0-0.2 wt%, and phosphorus content is 0-0.1 wt%, In particular, when the sodium content is 0.001-0.2% by weight, silicon content is 0.00005-0.005% by weight, chlorine content is 0.001-0.05% by weight, potassium content is 0.003-0.15% by weight, and phosphorus content is 0.002-0.1% by weight, 0.01-0.2% by weight of sodium, 0.0001-0.005% by weight of silicon, 0.005-0.05% by weight of chlorine, 0.01-0.15% by weight of potassium, and 0.01-0.1% by weight of phosphorus, or b) When the total content of the virus inactivation solution is 100% by weight, 10-60% by weight sodium, 0.2-5% by weight silicon, 5-15% by weight chlorine, 0-50% by weight potassium, and 0-40% by weight phosphorus; or When sodium is 20-50% by weight, silicon is 0.5-0.9% by weight, chlorine is 7-12% by weight, potassium is 20-40% by weight, and phosphorus is 10-30% by weight It was found that this method can achieve excellent virus inactivation.

[0093] Furthermore, when the weight ratio of phosphorus to silicon in the original solution is 10-50, or even 20-40, or / and When the ratio of the total weight of sodium and potassium to the total weight of phosphorus, silicon, and chlorine is 1-3, or even 1.5-2.5 It was found that even better virus inactivation could be achieved.

Claims

1. A virus inactivating agent capable of inactivating viruses, comprising: The virus inactivating agent is i) Alkaline ionized water containing purified water and at least sodium, silicon, chlorine, potassium, and phosphorus as components, containing more than 99.5% by weight of the purified water and having a pH of 10-13, or ii) a diluted solution obtained by diluting the alkaline ionized water with purified water in an amount of not more than twice the volume of the alkaline ionized water; and When the virus inactivating agent is taken as 100% by weight, the virus inactivating agent contains 0.01-0.2% by weight of sodium, 0.0001-0.005% by weight of silicon, 0.005-0.05% by weight of chlorine, 0.01-0.15% by weight of potassium, and 0.01-0.1% by weight of phosphorus; The virus to be inactivated by the virus inactivating agent is SARS-CoV-2. Virus inactivator.

2. 2. The virus inactivating agent according to claim 1, wherein the content of the purified water in the alkaline ionized water is 99.7% by weight or more.

3. A virus inactivation agent capable of inactivating viruses, comprising alkaline ionized water having a pH of 10-13, The alkaline ionized water contains at least sodium, silicon, chlorine, potassium, and phosphorus as its components, When the virus inactivating agent is taken as 100% by weight, the virus inactivating agent contains 0.01-0.2% by weight of sodium, 0.0001-0.005% by weight of silicon, 0.005-0.05% by weight of chlorine, 0.01-0.15% by weight of potassium, and 0.01-0.1% by weight of phosphorus; The virus to be inactivated by the virus inactivating agent is SARS-CoV-2. Virus inactivator.

4. The virus inactivating agent according to claim 3 , wherein the alkaline ionized water is alkaline ionized water.

5. 5. The virus inactivating agent according to claim 3, wherein a total amount of ingredients contained in the virus inactivating agent is 0.001% by weight or more and 2% by weight or less when the virus inactivating agent is taken as 100% by weight.

6. 6. The virus inactivating agent according to claim 1, wherein the weight ratio of phosphorus to silicon in the virus inactivating agent is 10-50.

7. 7. The virus inactivating agent according to claim 1, wherein the ratio of the total weight of sodium and potassium to the total weight of phosphorus, silicon and chlorine is 1-3.

8. The virus inactivating agent according to any one of claims 1 to 7, wherein the virus inactivating agent is a virus inactivating solution.

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