Grape plants, methods for producing grape plants, and uses thereof

Abstract

To provide a new grape plant option which has a red skin color, good taste, and can be cultivated seedlessly. [Solution] In one aspect of the present invention, a method for producing grape plants is provided for crossing grape varieties to determine whether the following genetic markers, TsuVv001, TsuVv002, TsuVv003, TsuVv013, TsuVv014, TsuVv016, TsuVv019, TsuVv025, TsuVv030, TsuVv031, TsuVv047, and VrZAG83, are AB type and VrZAG83 are AB type, respectively, in a hybrid variety obtained by crossing grape varieties. and selecting a plant having the following DNA marker types: TsuVv02 is type C, TsuVv003 is type ABC, TsuVv013 is type B, TsuVv014 is type BC, TsuVv016 is type B, TsuVv019 is type BC, TsuVv025 is type AB, TsuVv030 is type ABC, TsuVv031 is type B, TsuVv047 is type AB, and VrZAG83 is type CEF.

Description

[Technical Field]

[0001] The present invention relates to a novel grape plant having red skin color, a method for producing the grape plant, and uses thereof. [Background technology]

[0002] The main grape varieties currently cultivated are largely dominated by the green-skinned Shine Muscat (Japan Variety Registration Number 13891), and the black-skinned Kyoho and Pione varieties (Non-Patent Document 1). [Prior art documents] [Non-patent literature]

[0003] [Non-Patent Document 1] Survey on the Trends in Production of Specialty Fruit Trees in 2021 (Ministry of Agriculture, Forestry and Fisheries) Summary of the Invention [Problem to be solved by the invention]

[0004] To expand demand for grapes, there is a need for new varieties that differ from the major varieties "Shine Muscat," "Kyoho," and "Pione" in terms of appearance, such as skin color, and that can be cultivated seedless and have good eating quality. In addition to these characteristics, there is also a high demand for early-ripening varieties that, for example, have a different fruit maturity period than the major varieties (for example, those that mature before mid-August in typical grape-growing regions in Japan). Thus, there is a high demand for new grape plants, methods for creating grape plants, and uses for them. In one aspect of the present invention, it is an object of the present invention to provide a new selection of grapevine plants that have, for example, red skin color, good taste, and can be cultivated seedless. In another aspect of the present invention, it is an object of the present invention to provide a new selection of grapevine plants that, in addition to these characteristics, have, for example, a new early-maturing grapevine plant that has a different fruit maturity time from that of major varieties. [Means for solving the problem]

[0005] The present invention includes, for example, the following. 1) A method for producing a grape plant, comprising: In hybrid varieties obtained by crossing grape varieties or their progeny plants, the following genetic markers TsuVv001, TsuVv002, TsuVv003, TsuVv013, TsuVv014, TsuVv016, TsuVv019, TsuVv025, TsuVv030, TsuVv031, TsuVv047, and VrZAG83 are identified as follows: TsuVv001 is type AB, TsuVv002 is type C, TsuVv003 is ABC type, TsuVv013 is type B, TsuVv014 is BC type, TsuVv016 is type B, TsuVv019 is type BC, TsuVv025 is type AB, TsuVv030 is ABC type, TsuVv031 is type B, TsuVv047 is type AB, and VrZAG83 is CEF type A method for producing grape plants, comprising the step of selecting plants having a DNA marker type. 2) A method for propagating grape plants, comprising a step of asexually propagating grape plants produced by the production method described in 1) above. 3) A method for producing a grape plant, comprising: A breeding step of breeding parents (1) and (2) shown below; Parents (1): Queen Nina, Parent (2): A hybrid line obtained by crossing a Kyoho cross with a Neo Muscat tetraploid cross with Shiramine. a selection step of selecting candidate grapevine plant lines obtained as a result of the crossbreeding step using genetic markers as indicators, In the selection process, one or more genetic markers selected from the group consisting of TsuVv002, TsuVv019, TsuVv003, TsuVv013, TsuVv014, TsuVv030, and VrZAG83 are used to select candidate grape plant lines that have different DNA marker type patterns of the genetic markers from those of the breeding parents (1) and (2). 4) A method for propagating grape plants, comprising a step of asexually propagating grape plants produced by the production method described in 3) above. 5) A grape plant, wherein the DNA marker type of the genetic marker in the grape plant is: TsuVv001 is type AB, TsuVv002 is type C, TsuVv003 is ABC type, TsuVv013 is type B, TsuVv014 is BC type, TsuVv016 is type B, TsuVv019 is type BC, TsuVv025 is type AB, TsuVv030 is ABC type, TsuVv031 is type B, TsuVv047 is type AB, and VrZAG83 is CEF type, The grape plant. 6) A novel grape plant, - Obtained by crossing the following breeding parents (1) and (2), Parents (1): Queen Nina, Parent (2): A hybrid line obtained by crossing a Kyoho cross with a Neo Muscat tetraploid cross with Shiramine. -The skin color is red, -The fruit ripens earlier than Queen Nina. - The fruit has good eating quality, and - distinguishable from other grapevine varieties, including the breeding parents (1) and (2), using one or more genetic markers selected from the group consisting of TsuVv001, TsuVv002, TsuVv003, TsuVv013, TsuVv014, TsuVv016, TsuVv019, TsuVv025, TsuVv030, TsuVv031, TsuVv047, and VrZAG83; Grape plant. [Effects of the Invention]

[0006] The present invention provides a new grape plant, a method for producing a grape plant, and uses thereof. One aspect of the present invention provides a new option for a new grape plant, for example, one with a red skin color, good taste, and capable of seedless cultivation. Another aspect of the present invention provides a new option for a new early-maturing grape plant that, in addition to the above characteristics, has a different fruit maturation time from that of major varieties. [Brief explanation of the drawings]

[0007] [Figure 1] FIG. 1 is a breeding lineage diagram of "grape AK31" shown in the examples. [Figure 2] FIG. 2 is a diagram showing a full view of the fruiting state of the "grape AK31" shown in the example. [Figure 3] FIG. 3 is a diagram showing a cluster of grape AK31 shown in the examples. [Figure 4] FIG. 4 shows waveforms obtained by analyzing DNA extracted from the respective reference varieties of TsuVv001, TsuVv002, TsuVv003, TsuVv013, and TsuVv014 with a DNA sequencer. [Figure 5] FIG. 5 shows waveforms obtained by analyzing DNA extracted from the respective reference varieties of TsuVv016, TsuVv019, TsuVv025, TsuVv030, and TsuVv031 with a DNA sequencer. [Figure 6]FIG. 6 shows waveforms obtained by analyzing DNA extracted from the respective reference varieties of TsuVv047 and VrZAG83 with a DNA sequencer. DETAILED DESCRIPTION OF THE INVENTION

[0008] The present invention will be described in detail below. All of the documents mentioned in this specification are incorporated herein by reference. As used herein, the term "plant" may refer to a part or the whole of a plant body, and may refer to any stage of plant development. As used herein, the term "traits" refers to the morphological and physiological characteristics of a plant. As used herein, SSR is an abbreviation for Simple Sequence Repeat, also known as microsatellite.

[0009] [1. Grape plant production method 1] In one embodiment of the method for producing a grape plant, the following genetic markers, TsuVv001, TsuVv002, TsuVv003, TsuVv013, TsuVv014, TsuVv016, TsuVv019, TsuVv025, TsuVv030, TsuVv031, TsuVv047, and VrZAG83, are detected in a hybrid variety obtained by crossing grape varieties or a progeny plant thereof, respectively: TsuVv001 is type AB, TsuVv002 is type C, TsuVv003 is ABC type, TsuVv013 is type B, TsuVv014 is BC type, TsuVv016 is type B, TsuVv019 is type BC, TsuVv025 is type AB, TsuVv030 is ABC type, TsuVv031 is type B, TsuVv047 is type AB, and VrZAG83 is CEF type A method for producing grape plants includes a step of selecting plants that have a DNA marker type.

[0010] In one embodiment, the plants used in the selection step may be hybrid varieties obtained by crossing grape varieties with each other, or their progeny plants, but are preferably hybrid varieties obtained by crossing grape varieties with each other. In one embodiment, the production method may further comprise a propagation step in which the grape plant is vegetatively propagated.

[0011] (Grape plant selection process) (A) Selection process using genetic markers In one embodiment, the production method involves selecting a hybrid variety or its progeny plants obtained by crossing grape varieties using genetic markers TsuVv001, TsuVv002, TsuVv003, TsuVv013, TsuVv014, TsuVv016, TsuVv019, TsuVv025, TsuVv030, TsuVv031, TsuVv047, and VrZAG83. For genetic markers (SSR markers) and selection methods, please refer to the method described in the "Selection process using genetic markers as indicators" section of the "Method for producing grape plants 2" described below, and the following documents (1) to (3). (1) Japanese Patent Publication: Patent No. 7373232 "Method for identifying grape varieties, polynucleotides, microsatellite markers and primer sets" (2) "DNA Variety Identification Technique Manual for 24 Grape Varieties - DNA Variety Identification Technique for 24 Grape Varieties Using SSR Markers - (National Agriculture and Food Research Organization) / First Edition, December 27, 2018 / National Agriculture and Food Research Organization, Fruit Tree and Tea Research Division, Seed and Seedling Management Center" (3) "DNA Variety Identification Technology for 24 Grape Varieties - DNA Variety Identification Technology for 24 Grape Varieties Using SSR Markers - (National Agriculture and Food Research Organization) / First Edition January 27, 2021 / National Agriculture and Food Research Organization, Fruit Tree and Tea Industry Research Division" The outline is as follows: First, DNA is extracted from hybrid varieties obtained by crossing grape varieties or their progeny using existing methods. Next, the extracted DNA is amplified by PCR using a pair of fluorescently labeled forward and reverse primers. The information on the forward and reverse primers for each genetic marker is as follows:

[0012] [Table 1]

[0013] The resulting PCR products are analyzed using a DNA sequencer to obtain waveforms (fragment patterns), which are then compared with the waveforms of predetermined reference varieties for each SSR marker to determine the DNA marker type. The reference variety is a single variety or a set of multiple varieties in which all possible patterns can be detected for each SSR marker. For example, the reference variety for the genetic marker TsuVv001 is Muscat Bailey A, and when this is analyzed with a DNA sequencer, the resulting waveform diagram contains two waveform patterns, A and B. If the hybrid variety or its progeny has a waveform corresponding to A, the DNA marker type of TsuVv001 will be A; if it has a waveform corresponding to B, the DNA marker type of TsuVv001 will be B. If it has both waveforms, the DNA marker type of TsuVv001 will be AB. For TsuVv002, four patterns, A, B, C, and D, were detected, but all four patterns could be detected in the three varieties, Koshu, Concord, and Delaware, and therefore these were used as the reference varieties. Similarly, the DNA marker types of TsuVv003, TsuVv013, TsuVv014, TsuVv016, TsuVv019, TsuVv025, TsuVv030, TsuVv031, TsuVv047, and VrZAG83 are determined by comparing them with the waveforms in the reference variety. Table 2 shows the reference varieties for each SSR marker, and Figures 4 to 6 show the waveforms of the reference varieties.

[0014] [Table 2] Tables 3 and 4 show the results of the genetic marker determination of each grape variety.

[0015] [Table 3]

[0016] [Table 4]

[0017] In one embodiment, the method of making comprises: TsuVv001 is type AB, TsuVv002 is type C, TsuVv003 is ABC type, TsuVv013 is type B, TsuVv014 is BC type, TsuVv016 is type B, TsuVv019 is type BC, TsuVv025 is type AB, TsuVv030 is ABC type, TsuVv031 is type B, TsuVv047 is type AB, and VrZAG83 is CEF type The method includes a step of selecting plants that have the DNA marker type.

[0018] In one embodiment, the production method may further include a step of crossbreeding the grape varieties used in the above hybridization, with the father (pollen parent) being "Queen Nina" and the mother (seed parent) being "a hybrid line obtained by crossing a hybrid line between Kyoho grapes with a hybrid line between a Neo Muscat tetraploid and Shiramine grapes," to obtain the hybrid variety. The "hybrid line obtained by crossing a hybrid line between Kyoho grapes with a hybrid line between a Neo Muscat tetraploid and Shiramine grapes" is preferably a hybrid line obtained by crossing a "hybrid line between Kyoho grapes" as a pollen parent and a "hybrid line between a Neo Muscat tetraploid and Shiramine grapes" as a seed parent. The "hybrid line between Neo Muscat tetraploid and Shiramine" is preferably a hybrid line obtained by crossing Neo Muscat tetraploid as a seed parent and Shiramine as a pollen parent. Queen Nina is registered under Japan Variety Registration Number 20733. Kyoho and Shiramine are common varieties widely distributed in Japan. Neo Muscat (tetraploid) can be produced by applying a 0.25% colchicine solution containing 5% glycerin and 20 ppm Airol OP to the axillary buds of the common Neo Muscat grapes, dripping the solution on the axillary buds of new shoots once every other day for three cycles, or once a day for five cycles. (For more information on Neo Muscat (tetraploid), see, for example, "Studies on Polyploid Breeding of Grapes" (Yamane Hiroyasu and Kurihara Akio, Bulletin of the Fruit Tree Research Station, Series E, Akitsu, Akitsu Branch of the Fruit Tree Research Station, Ministry of Agriculture, Forestry and Fisheries, March 1980, No. 3, p. 10). A representative example of a "Kyoho cross line" is Akitsu No. 14. Akitsu No. 14 has the DNA marker types shown in Tables 3 and 4. In the "Kyoho cross line", TsuVv001 is preferably AB type, TsuVv002 is type C, TsuVv003 is AB type, TsuVv013 is AB type, TsuVv014 is type C, TsuVv016 is type B, TsuVv019 is type BC, TsuVv025 is type AB, TsuVv030 is type AC, TsuVv031 is type B, TsuVv047 is type AB, and VrZAG83 is type EF. A representative example of a "hybrid line between Neo Muscat tetraploid and Shiramine" is Akitsu 22. Akitsu 22 has the DNA marker types shown in Tables 3 and 4. In the "hybrid line between Neo Muscat tetraploid and Shiramine", TsuVv001 is preferably AB type, TsuVv002 is AC type, TsuVv003 is ABC type, TsuVv013 is AB type, TsuVv014 is BC type, TsuVv016 is B type, TsuVv019 is BC type, TsuVv025 is AB type, TsuVv030 is ABC type, TsuVv031 is B type, TsuVv047 is AB type, and VrZAG83 is CE type. A representative example of a hybrid line obtained by crossing a Kyoho grape hybrid with a Neo Muscat tetraploid and Shiramine grape hybrid is 396-56. 396-56 has the DNA marker types shown in Tables 3 and 4. It is preferable that the "hybrid line obtained by crossing a hybrid line between Kyoho grapes with a hybrid line between a Neo Muscat tetraploid and Shiramine grapes" be such that TsuVv001 is type AB, TsuVv002 is type AC, TsuVv003 is type ABC, TsuVv013 is type B, TsuVv014 is type BC, TsuVv016 is type B, TsuVv019 is type C, TsuVv025 is type AB, TsuVv030 is type ABC, TsuVv031 is type B, TsuVv047 is type AB, and VrZAG83 is type CEF.

[0019] Grapevine plants selected in the selection process have the DNA marker type pattern of the above genetic markers. In contrast, in another grapevine variety, "Queen Nina," TsuVv003 is type AB, TsuVv013 is type AB, TsuVv014 is type AC, TsuVv030 is type AB, and VrZAG83 is type EF. Therefore, "Queen Nina" can be distinguished from other grapevine varieties using TsuVv003, TsuVv013, TsuVv014, TsuVv030, and / or VrZAG83. Furthermore, in the grape variety "Neo Muscat (tetraploid)," TsuVv001 is type A, TsuVv002 is type AC, TsuVv003 is type C, TsuVv013 is type AB, TsuVv030 is type AB, TsuVv047 is type A, and VrZAG83 is type CE. Therefore, TsuVv001, TsuVv002, TsuVv003, TsuVv013, TsuVv030, TsuVv047, and / or VrZAG83 can be used to distinguish from other grape varieties "Neo Muscat." Furthermore, in another grape variety, "Shiramine," TsuVv003 is type AB, TsuVv013 is type AB, and TsuVv014 is type C. Therefore, "Shiramine" can be distinguished from other grape varieties using TsuVv003, TsuVv013, and / or TsuVv014. Furthermore, in another grape variety, "Kyoho," TsuVv003 is type AB, TsuVv013 is type AB, and TsuVv014 is type C. Therefore, TsuVv003, TsuVv013, and / or TsuVv014 can be used to distinguish from other grape varieties, "Kyoho." In addition, in another grape variety, "396-56," TsuVv02 is of the AC type and TsuVv019 is of the CC type. Therefore, the grape variety "396-56" can be distinguished from other grape varieties using TsuVv02 and / or TsuVv019. In addition, in another grape variety, "Akitsu No. 14," TsuVv003 is AB type, TsuVv013 is AB type, TsuVv014 is C type, TsuVv030 is AC type, and VrZAG83 is EF type. Therefore, "Akitsu No. 14" can be distinguished from other grape varieties using TsuVv003, TsuVv013, TsuVv014, TsuVv030, and / or VrZAG83. In addition, in another grape variety, "Akitsu 22," TsuVv002 is type AC, TsuVv013 is type AB, and VrZAG83 is type CE. Therefore, "Akitsu 22" can be distinguished from other grape varieties using TsuVv002, TsuVv013, and / or VrZAG83.

[0020] (Estimation of parent lineage) The DNA marker types listed in Tables 3 and 4 represent the alleles (genes) detected for each variety at each genetic marker. Because grapevines are diploid, triploid, or tetraploid plants, up to four alleles can be detected for each genetic marker. However, at least one allele found in the offspring must be inherited from each seed parent and pollen parent. For example, if a variety has the AB genotype, it is possible that one of the parents has an A or B allele and is a potential breeding parent. However, if both parents are BB, this combination is ruled out as a possible parent. Based on this principle, potential breeding parents can be estimated from information on 12 genetic markers.

[0021] (B) A selection process based on specific traits In one embodiment, the production method may further include a selection step based on a specific trait. The selection step based on a specific trait may be performed together with a selection step using a genetic marker as an index. In a preferred example, after the selection step using a genetic marker as an index, a selection step based on a specific trait is performed on candidate grape plant lines that have been sufficiently narrowed down. The main traits used in this selection process are characteristics related to grape berries. For example, the selection process is based on one or more, preferably all, of the following characteristics: 1) red skin color, 2) early ripening (early maturity), 3) resistance to cracking, 4) firm flesh (not soft flesh), 5) flesh characteristics that are more disintegrative than lumpy (easy to chew), 6) pleasant fruit aroma, and 7) good eating quality. Regarding 7), good eating quality, while sensory testing can be performed, it can also be evaluated using the balance between sugar content and acidity as an indicator. Furthermore, for lines with excellent grape berry characteristics, a selection process can be further performed, if necessary, using the degree of seedlessness achieved by gibberellin treatment as an indicator to select grape plants particularly suitable for seedless cultivation. The selection process can also be carried out using traits related to the ease of growing grape plants (for example, vigor, disease resistance, degree of flower drop, etc.). In one embodiment, the method of production preferably involves selecting grape plants with the trait of early maturity for the grape variety "Queen Nina."

[0022] (Grape plant propagation) The grape plants grown through the crossbreeding and selection steps are optionally subjected to a propagation step in which they are propagated asexually. Methods of asexual propagation are not particularly limited, and include, for example, grafting (which may involve cross-grafting onto other grape plants (Vitis L.)), cuttings, or cell culture. Asexual propagation is also called unsexual propagation, asexual reproduction, or vegetative propagation. Asexual propagation does not require males or females, and is a method in which a single individual independently forms a new individual, with reproductive cells produced in a single individual independently becoming a new individual. Furthermore, techniques for creating cell cultures of plant tissues and methods for regenerating plants from tissue cultures are carried out using known techniques. In the present invention, the rootstocks to be used for the grafting may be, for example, the Teleki rootstocks of Berlandieri x riparia (Teleki 5BB, Teleki 8B, Teleki 5C, etc.); other rootstocks of Berlandieri x riparia (SO4 rootstock, 420A rootstock, etc.); Riparia x rupestris rootstocks (3309 rootstock, 101-14 rootstock, etc.); or any rootstock that can be used as a rootstock for grape plants, taking into consideration the characteristics of the cultivation area, etc.

[0023] [2. Grape plant production method 2] (overview) The method for producing grapevine plants according to one embodiment of the present invention includes the steps of: 1) preparing a breeding parent by selecting or producing a breeding parent from an existing grapevine variety; 2) crossbreeding the breeding parents; and 3) selecting a grapevine plant of interest from the candidate plants obtained in the crossbreeding step using genetic markers as indicators. The method for producing grapevine plants can also be referred to as a method for producing, cultivating, or producing grapevine plants.

[0024] (Preparation of parents for breeding) The breeding parents for producing the grapevine plant of this embodiment are either selected from specific existing varieties or obtained by crossbreeding specific existing varieties. One of the breeding parents (particularly the pollen parent) can be obtained, for example, by crossing hybrid line A obtained by crossbreeding two "Kyoho" varieties with hybrid line B obtained by crossbreeding "Benzuiho" and "Shiramine" (preferably a crossbreeding in which "Benzuiho" is the seed parent and "Shiramine" is the pollen parent). A typical example of a pollen parent is "Queen Nina (Japan Variety Registration No. 20733)," which is easily selected as the pollen parent. A typical example of hybrid line A is "Aki Queen (Japan Variety Registration No. 3458)." The cross between hybrid line A and hybrid line B is preferably a crossbreeding in which hybrid line A is the pollen parent and hybrid line B is the seed parent. The other of the breeding parents (particularly the seed parent) is, for example, a hybrid line obtained by crossing hybrid line C obtained by crossing two "Kyoho" varieties with hybrid line D obtained by crossing "Neo Muscat (tetraploid)" with "Shiramine" (preferably a cross in which "Neo Muscat (tetraploid)" is the seed parent and "Shiramine" is the pollen parent). The cross between hybrid line C and hybrid line D is preferably a cross in which hybrid line C is the pollen parent and hybrid line D is the seed parent. Note that the breeding parents can also be identified and selected using publicly known genetic markers (SSR markers) described below as indicators. It is preferable that the breeding parents be obtained by crossing a father (pollen parent) of "Queen Nina" and a mother (seed parent) of "a hybrid line obtained by crossing a hybrid line between Kyoho grapes with a hybrid line between a Neo Muscat tetraploid and Shiramine grape." Furthermore, the "hybrid line obtained by crossing a hybrid line between Kyoho grapes with a hybrid line between a Neo Muscat tetraploid and Shiramine grapes" is preferably a hybrid line obtained by crossing a "hybrid line between Kyoho grapes" as a pollen parent and a "hybrid line between a Neo Muscat tetraploid and Shiramine grapes" as a seed parent. Furthermore, the "hybrid line between Neo Muscat tetraploid and Shiramine" is preferably a hybrid line obtained by crossing Neo Muscat tetraploid as a seed parent and Shiramine as a pollen parent.

[0025] (Mating process of parents) Next, the prepared breeding parents (pollen parent and seed parent) are crossed to obtain a candidate grape plant line. Specifically, for example, grape seeds obtained from the fruit obtained by crossing are sown, and young plants (own-rooted seedlings) of the candidate grape plant are cultivated. Then, scion is taken from the own-rooted seedlings that have grown to a certain size to create grafted seedlings, which are then planted in a field. The planted grafted seedlings of the candidate grape plant are subjected to the selection process described below. Note that the selection process using genetic markers as an indicator can also be carried out early on, for example, on the above-mentioned young plants (own-rooted seedlings). In this case, the labor and costs required for later processes (such as creating grafted seedlings and planting them in a field) can be significantly reduced.

[0026] (Grape plant selection process) (A) Selection process using genetic markers To obtain the grape plant according to the present embodiment from a candidate grape plant line, a selection method using known genetic markers (SSR markers) as indicators, as disclosed in the following documents (1) to (3), can be applied. The contents of these documents (1) to (3) are all incorporated herein by reference. See also the Examples section for the DNA marker type patterns of genetic markers of the grape plant according to the present embodiment and other major grape plants. (1) Japanese Patent Publication: Patent No. 7373232 "Method for identifying grape varieties, polynucleotides, microsatellite markers and primer sets" (2) "DNA Variety Identification Technique Manual for 24 Grape Varieties - DNA Variety Identification Technique for 24 Grape Varieties Using SSR Markers - (National Agriculture and Food Research Organization) / First Edition, December 27, 2018 / National Agriculture and Food Research Organization, Fruit Tree and Tea Research Division, Seed and Seedling Management Center" (3) "DNA Variety Identification Technology for 24 Grape Varieties - DNA Variety Identification Technology for 24 Grape Varieties Using SSR Markers - (National Agriculture and Food Research Organization) / First Edition January 27, 2021 / National Agriculture and Food Research Organization, Fruit Tree and Tea Industry Research Division" Note that documents (1) to (3) all relate to a common technique, using the same 12 SSR markers (TsuVv001, TsuVv002, TsuVv003, TsuVv013, TsuVv014, TsuVv016, TsuVv019, TsuVv025, TsuVv030, TsuVv031, TsuVv047, and VrZAG83) and the same method for detecting SSR markers. Document (3) also relates to extracting DNA samples from grape berries (skin). To obtain grapevine plants according to this embodiment, the DNA marker type patterns of all 12 SSR markers described above may be determined, but depending on the purpose of selection, only a portion of the 12 SSR markers may be used. For example, to determine whether a grapevine has a DNA marker type pattern different from that of its parent, one, two, three, four, five, six, or seven SSR markers selected from the group consisting of TsuVv002, TsuVv019, TsuVv003, TsuVv013, TsuVv014, TsuVv030, and VrZAG83 can be used. In a more specific example, a combination of one or more SSR markers selected from the group consisting of TsuVv002 and TsuVv019 and one or more SSR markers selected from the group consisting of TsuVv003, TsuVv013, TsuVv014, TsuVv030, and VrZAG83 can be used. In a typical example of a grape plant according to this embodiment, the DNA marker type patterns of the 12 types of SSR markers are as follows: TsuVv001 is type AB, TsuVv002 is type C, TsuVv003 is type ABC, TsuVv013 is type B, TsuVv014 is type BC, TsuVv016 is type B, TsuVv019 is type BC, TsuVv025 is type AB, TsuVv030 is type ABC, TsuVv031 is type B, TsuVv047 is type AB, and VrZAG83 is type CEF. Furthermore, when focusing on the seven SSR markers TsuVv002, TsuVv019, TsuVv003, TsuVv013, TsuVv014, TsuVv030, and VrZAG83, in a typical example of a grape plant according to this embodiment, TsuVv002 is type C, TsuVv019 is type BC, TsuVv003 is type ABC, TsuVv013 is type B, TsuVv014 is type BC, TsuVv030 is type ABC, and VrZAG83 is type CEF. Furthermore, as shown in the examples, by determining the DNA marker type patterns of some or all of the 12 SSR markers mentioned above, the grape plants of this embodiment and their cross parents can be distinguished from at least the 24 major cultivated varieties described below. The 24 main cultivars are Aki Queen, Campbell Early, Kyoho, Queen Nina, Grosskrone (Japan Variety Registration No. 28086), Koshu, Concord, San Verde (Japan Variety Registration No. 20821), Shine Muscat, Suiho (Japan Variety Registration No. 5075), Steuben, Sekirei, Takao, Delaware, Niagara, Nagano Purple (Japan Variety Registration No. 12074), Pione, Fujiminori (Japan Variety Registration No. 919), Black Beat (Japan Variety Registration No. 12075), Portland, Muscat of Alexandria, Muscat Bailey A, Ruby Roman (Japan Variety Registration No. 15020), and Rosario Bianco. Furthermore, a person skilled in the art can easily understand, based on the results of the examples, the SSR markers that should be selected to distinguish the grape plant of this embodiment or its hybrid parent from specific major cultivated varieties.

[0027] (B) A selection process based on specific traits To obtain the grapevine plant according to the present embodiment from candidate grapevine plant lines, a selection step based on specific traits can be performed. The selection step based on specific traits can also be performed together with a selection step using genetic markers as indicators. In a preferred example, after the selection step using genetic markers as indicators has been performed, the selection step based on specific traits is performed on the candidate grapevine plant lines that have been sufficiently narrowed down. The main traits used in this selection process are characteristics related to grape berries. For example, the selection process is based on one or more, preferably all, of the following characteristics: 1) red skin color, 2) early ripening (early maturity), 3) resistance to cracking, 4) firm flesh (not soft flesh), 5) flesh characteristics that are more disintegrative than lumpy (easy to chew), 6) pleasant fruit aroma, and 7) good eating quality. Regarding 7), good eating quality, while sensory testing can be performed, it can also be evaluated using the balance between sugar content and acidity as an indicator. Furthermore, for lines with excellent grape berry characteristics, a selection process can be further performed, if necessary, using the degree of seedlessness achieved by gibberellin treatment as an indicator to select grape plants particularly suitable for seedless cultivation. The selection process can also be carried out using traits related to the ease of growing grape plants (for example, vigor, disease resistance, degree of flower drop, etc.).

[0028] (Grape plant propagation) The grape plants grown through the crossbreeding and selection steps are optionally subjected to a propagation step in which they are propagated asexually. Methods of asexual propagation are not particularly limited, and include, for example, grafting (which may involve cross-grafting onto other grape plants (Vitis L.)), cuttings, or cell culture. Asexual propagation is also called unsexual propagation, asexual reproduction, or vegetative propagation. Asexual propagation does not require males or females, and is a method in which a single individual independently forms a new individual, with reproductive cells produced in a single individual independently becoming a new individual. Furthermore, techniques for creating cell cultures of plant tissues and methods for regenerating plants from tissue cultures are carried out using known techniques. In the present invention, the rootstocks to be used for the grafting may be, for example, the Teleki rootstocks of Berlandieri x riparia (Teleki 5BB, Teleki 8B, Teleki 5C, etc.); other rootstocks of Berlandieri x riparia (SO4 rootstock, 420A rootstock, etc.); Riparia x rupestris rootstocks (3309 rootstock, 101-14 rootstock, etc.); or any rootstock that can be used as a rootstock for grape plants, taking into consideration the characteristics of the cultivation area, etc.

[0029] [3. Grape plants and their processed products] (Grape plant 1) In one embodiment of the present invention, the DNA marker type of the genetic marker in the grape plant is: TsuVv001 is type AB, TsuVv002 is type C, TsuVv003 is ABC type, TsuVv013 is type B, TsuVv014 is BC type, TsuVv016 is type B, TsuVv019 is type BC, TsuVv025 is type AB, TsuVv030 is ABC type, TsuVv031 is type B, TsuVv047 is type AB, and VrZAG83 is CEF type, The grape plant. As is clear from Tables 3 and 4, the grape plant according to one embodiment of the present invention has a different DNA marker type of genetic markers from other grape varieties. Therefore, the grape plant according to one embodiment of the present invention is a novel grape plant.

[0030] In one embodiment, the grape plant may be a hybrid variety or a descendant plant thereof, which is a hybrid of a father (pollen parent) of "Queen Nina" and a mother (seed parent) of "a hybrid line obtained by crossing a hybrid line between Kyoho grapes with a hybrid line between a Neo Muscat tetraploid and Shiramine grapes." The "hybrid line obtained by crossing a hybrid line between Kyoho grapes with a hybrid line between a Neo Muscat tetraploid and Shiramine grapes" is preferably a hybrid line obtained by crossing a "hybrid line between Kyoho grapes" as a pollen parent and a "hybrid line between a Neo Muscat tetraploid and Shiramine grapes" as a seed parent. The "hybrid line between Neo Muscat tetraploid and Shiramine" is preferably a hybrid line obtained by crossing Neo Muscat tetraploid as a seed parent and Shiramine as a pollen parent. In one embodiment, the grapevine plants preferably have the trait of early maturity for the grape variety "Queen Nina." For further details, please refer to the section on how to create grape plants. (Grape plant 2) A grape plant according to another embodiment of the present invention is obtained by crossing the breeding parents shown in the "Method for Producing Grape Plants" section. A typical example of one of the breeding parents is, but is not limited to, Queen Nina, which is selected as the pollen parent. A typical example of the other breeding parent is, but is not limited to, a hybrid line obtained by crossing line C obtained by crossing Kyoho grapes with line D obtained by crossing Neo Muscat (tetraploid) with Shiramine grapes, which is selected as the seed parent. For further details, please refer to the section on methods for producing grape plants. Preferred embodiments of grape plant 1 and / or grape plant 2 will be described below.

[0031] (Main characteristics of grapevine fruit) The grape plant according to this embodiment has a red skin color. A red skin color is, for example, a red in the range of pale pink (light red) to purple red, and typically a red in the range of pale pink (light red) to crimson (red). The grape plant according to this embodiment has a skin color similar to that of Aki Queen. The grape plant according to this embodiment has a skin color similar to that of Queen Nina, or a slightly lighter red. The grape plant according to this embodiment is an early-ripening variety. The grape plant according to this embodiment has fruit maturity earlier than Queen Nina, typically about 7 to 10 days or about 5 to 10 days earlier than Queen Nina. The grape plant according to this embodiment has fruit maturity earlier than Aki Queen, typically about 3 to 7 days or about 4 to 7 days earlier than Aki Queen. In typical cultivation areas in Japan, the grape plant according to this embodiment has fruit maturity before mid-August, for example, from early August to around August 14th. The grape plant according to this embodiment produces fruit with good eating quality. Fruit eating quality can be evaluated by sensory evaluation, but sugar content, acidity, and / or the balance between sugar content and acidity can also be used to numerically evaluate eating quality. The grape plant according to this embodiment is characterized by a high sugar content (%) of the fruit, and the sugar content (%) of the fruit is, for example, 18 or more, 18.5 or more, 19 or more, 19.5 or more, 19.6 or more, 19.7 or more, 19.8 or more, 19.9 or more, 20 or more, 20.1 or more, 20.2 or more, 20.3 or more, 20.4 or more, or 20.5 or more. The upper limit of the sugar content (%) of the fruit is not particularly limited, but is, provided that it is equal to or greater than any of the lower limits, for example, 22 or less, 21 or less, or 20.5 or less. The grape plant according to this embodiment is characterized by a low fruit acidity (g / 100 ml), and the fruit acidity (g / 100 ml) is, for example, 0.4 or less, less than 0.4, 0.39 or less, 0.38 or less, 0.37 or less, 0.36 or less, 0.35 or less, 0.34 or less, 0.33 or less, 0.32 or less, 0.31 or less, 0.30 or less, or 0.29 or less. The lower limit of the fruit acidity (g / 100 ml) is not particularly limited, but is, provided that it is a value equal to or less than one of the upper limits, for example, 0.25 or more, 0.29 or more, or 0.30 or more. The grape plant according to this embodiment tends to have a higher sugar content and lower acidity than Aki Queen. In terms of the balance between the sugar content (%) and acidity (g / 100ml) of the fruit of the grape plant of this embodiment, assuming that the units of both indicators are omitted for comparison, when the acidity is set to 1, the sugar content is, for example, in the range of 50 to 75, typically in the range of 50 to 60, in the range of 51 to 57, or in the range of 52 to 56. The grape plant according to this embodiment produces large berries, with a berry weight ranging from 9g to 13g per berry, typically ranging from 9.5g to 12g per berry, or ranging from 9.5g to 11.5g per berry. The grape plant according to this embodiment produces berries that are the same size as Aki Queen, or slightly larger than Aki Queen. Queen Nina produces berries that are larger than those of the grape plant according to this embodiment. The grapevine plants according to the present embodiment are excellent in terms of seedlessness (seedlessness) due to gibberellin treatment, and for example, two gibberellin treatments, one at full bloom and one 10 to 15 days after full bloom, can produce virtually all seedless grapes. Furthermore, if necessary, seedlessness can be more reliably achieved by combining this with a pre-flowering spray of streptomycin. The characteristics of the fruit of the grape plant according to the present embodiment described herein were evaluated using the methods described in the Examples below. The characteristics of the fruit described herein are particularly apparent when the fruit is made seedless by gibberellin treatment. The grape plant according to the present embodiment has an appearance different from that of varieties such as "Shine Muscat" and "Kyoho," matures in mid-August, has good taste, and can be an option for cultivating seedless varieties.

[0032] (DNA marker type of grape plants) Furthermore, the grape plant of this embodiment can be distinguished from other grape varieties, including the breeding parents, using one or more genetic markers selected from the group consisting of TsuVv001, TsuVv002, TsuVv003, TsuVv013, TsuVv014, TsuVv016, TsuVv019, TsuVv025, TsuVv030, TsuVv031, TsuVv047, and VrZAG83 (preferably by using all of the genetic markers). In a typical example of a grape plant according to this embodiment, the DNA marker type patterns of the 12 types of SSR markers are as follows: TsuVv001 is type AB, TsuVv002 is type C, TsuVv003 is type ABC, TsuVv013 is type B, TsuVv014 is type BC, TsuVv016 is type B, TsuVv019 is type BC, TsuVv025 is type AB, TsuVv030 is type ABC, TsuVv031 is type B, TsuVv047 is type AB, and VrZAG83 is type CEF. Furthermore, when focusing on the seven SSR markers TsuVv002, TsuVv019, TsuVv003, TsuVv013, TsuVv014, TsuVv030, and VrZAG83, in a typical example of a grape plant according to this embodiment, TsuVv002 is type C, TsuVv019 is type BC, TsuVv003 is type ABC, TsuVv013 is type B, TsuVv014 is type BC, TsuVv030 is type ABC, and VrZAG83 is type CEF.

[0033] (Various forms of grape plants) The grape plant may be a part or the whole of a plant. In one example, the grape plant is a cell isolated from a plant. In another example, the grape plant is a part of a plant selected from the group consisting of the root, stem, leaf, tendril, inflorescence or individual flower, fruit, seed, etc. Thus, the grape plant may be the fruit of a grape plant. Fruit as a part of a grape plant will now be further described. A fruit may be, for example, a grape cluster consisting of multiple berries, or an isolated berry detached from the cluster. A grape cluster may be, for example, an entire cluster, or a cut-off portion of the cluster. A fruit is typically separated (i.e., harvested) from the main body of a grape plant. A fruit may be seeded (with seeds) or seedless (seedless). One of the features of the grape plant according to this embodiment is that it is highly likely to be seedless by appropriate gibberellin treatment or the like. Fruit, such as a grape cluster or isolated berries, may be packaged in an appropriate packaging material. The form of the packaging material is not particularly limited, but examples include films, sheets, bags, and pouches. The material of the packaging material is not particularly limited, but examples include paper and resin (plastic). Packages of isolated grape berries typically contain multiple berries, for example, 5 or more, 10 or more, 20 or more, 30 or more, 40 or more, or 50 or more berries. The upper limit of the number of berries contained in a package of isolated grape berries is not particularly limited, and may be, for example, 500 or less, 400 or less, 300 or less, 200 or less, or 100 or less. Packages of grape berries may contain only one type of grape berry, or a combination of multiple types of grape berries. Packages of grape berries may also contain agricultural products other than grape berries. A product label may be attached to grapes (including grape packaging). The product label may record at least one piece of information selected from the following: grape variety name, intellectual property information related to grapes, grape producer information, grape production history information (e.g., information on pesticide spraying), grape distribution history information, product price, harvest date, and ripe-for-eating period information. This information may be recorded directly on the product label (by printing or, for example, electromagnetic recording), or may be readably recorded using a code format such as a one-dimensional code or a two-dimensional code. The grape plant may be in the form of vegetative propagation material such as a seedling or a scion. The seedling or scion may have a product label attached thereto. The product label contains at least one piece of information (product information) selected from the following: the name of the grape variety, intellectual property information related to grapes, individual identification information (ID number) of the seedling or scion, genetic marker information of the seedling or scion (e.g., the DNA marker pattern of the SSR marker described above), producer information of the seedling or scion, production history information of the seedling or scion (e.g., information on pesticide spraying), distribution history information of the seedling or scion, and product price. In a typical example, at least the individual identification information (ID number) of the seedling or scion is recorded. In another typical example, at least the individual identification information (ID number) of the seedling or scion and genetic marker information of the seedling or scion (e.g., the DNA marker pattern of the SSR marker described above) are recorded. This information may be directly recorded on the product label (either printed or readable electromagnetically), or readably recorded using a code format such as a one-dimensional code or a two-dimensional code. The product label may also be made up of a small electromagnetic recording medium such as an IC chip, embedded inside the seedling or scion. The location where the small electromagnetic recording medium such as an IC chip is embedded is not particularly limited. However, in the case of a grafted seedling, a preferred example is within the rootstock, and another preferred example is within a predetermined distance from the grafting position. A predetermined distance from the grafting position may be, for example, within 10 cm, 5 cm, 4 cm, 3 cm, 2 cm, or 1 cm from the grafting position. The predetermined distance may be from the rootstock toward the grafting position, or from the scion toward the grafting position toward the scion.

[0034] (Processed products of grape fruits, etc.) Processed products made from the fruit of grape plants according to the present embodiment are also included in the scope of the present invention. The types of processed fruit products are not particularly limited, but examples include grape juice, wine, jam, raisins, jelly, cake, fruit tart, and other Japanese and Western confectioneries. In the processed products, the fruit may be cut or peeled. The processed products are preferably edible processed products. Among these, the processed product is preferably at least one selected from the group consisting of grape juice, jam, jelly, cake, and fruit tart, and more preferably at least one selected from the group consisting of grape juice, jelly, and cake. [4. Product information management system for grapevine seedlings and scions (vegetative propagation materials)] In the above section [3. Grape plants, processed products thereof, etc.], we have explained the grape plant seedlings and scions according to this embodiment with product labels attached. The scope of the present invention also includes a product information management system for grape plant seedlings and scions according to this embodiment with product labels attached. An example of a product information management system includes at least 1) a grapevine seedling or cutting according to this embodiment with a product label attached thereto, and 2) a reader (an example of a client terminal) that reads the product information recorded on the product label. The product information management system may further include 3) a server. The product information management system may include a client terminal other than the reader, or may include a client terminal instead of the reader. These client terminals may not have the function of reading product information. In one example, the client terminal (which may be a reader) transmits a set of information recorded on the product label (especially the individual identification information (ID number) of the seedling or scion) and location information of the client terminal to the server. The client terminal (which may be a reader) may also transmit to the server information such as owner information of the seedling or scion (which may be the owner information of the client terminal if it matches the owner of the client terminal), location information of the seedling or scion (if different from the location information of the client terminal), etc. This allows information regarding the location, etc. of each individual seedling or scion to be managed. Furthermore, when the management environment of the seedling or scion changes, such as when the owner of the seedling or scion changes, when the location of the seedling or scion is relocated, or when the seedling or scion is discarded, this information is transmitted from the client terminal to the server.

[0035] [4. Summary] The present disclosure may include the following. 1) A method for producing a grape plant, comprising: In hybrid varieties obtained by crossing grape varieties, the following genetic markers TsuVv001, TsuVv002, TsuVv003, TsuVv013, TsuVv014, TsuVv016, TsuVv019, TsuVv025, TsuVv030, TsuVv031, TsuVv047, and VrZAG83 are respectively: TsuVv001 is type AB, TsuVv002 is type C, TsuVv003 is ABC type, TsuVv013 is type B, TsuVv014 is BC type, TsuVv016 is type B, TsuVv019 is type BC, TsuVv025 is type AB, TsuVv030 is ABC type, TsuVv031 is type B, TsuVv047 is type AB, and VrZAG83 is CEF type A method for producing grape plants, comprising the step of selecting plants having a DNA marker type. 2) The method of producing the hybrid variety described in 1), further comprising the step of crossbreeding the father (pollen parent) with "Queen Nina" and the mother (seed parent) with a "hybrid line obtained by crossing a hybrid line between Kyoho grapes with a hybrid line between a Neo Muscat tetraploid and Shiramine grapes" to obtain the hybrid variety. 3) The method according to 2), wherein the "hybrid line obtained by crossing a hybrid line between Kyoho grapes with a hybrid line between a Neo Muscat tetraploid and Shiramine grapes" is a hybrid line obtained by crossing a "hybrid line between Kyoho grapes" as a pollen parent and a "hybrid line between a Neo Muscat tetraploid and Shiramine grapes" as a seed parent. 4) The method for producing a hybrid line between a Neo Muscat tetraploid and Shiramine, as described in 2) or 3), wherein the hybrid line is a hybrid line obtained by crossing a Neo Muscat tetraploid as a seed parent with Shiramine as a pollen parent. 5) The method of any one of 2) to 4), wherein the "hybrid line obtained by crossing a hybrid line between Kyoho grapes with a hybrid line between a Neo Muscat tetraploid and Shiramine grapes" has TsuVv001 of type AB, TsuVv002 of type AC, TsuVv003 of type ABC, TsuVv013 of type B, TsuVv014 of type BC, TsuVv016 of type B, TsuVv019 of type C, TsuVv025 of type AB, TsuVv030 of type ABC, TsuVv031 of type B, TsuVv047 of type AB, and VrZAG83 of type CEF. 6) The method of any one of 2) to 5), wherein the "hybrid strain between Neo Muscat tetraploid and Shiramine" is such that TsuVv001 is AB type, TsuVv002 is AC type, TsuVv003 is ABC type, TsuVv013 is AB type, TsuVv014 is BC type, TsuVv016 is B type, TsuVv019 is BC type, TsuVv025 is AB type, TsuVv030 is ABC type, TsuVv031 is B type, TsuVv047 is AB type, and VrZAG83 is CE type. 7) The method of any one of 2) to 6), wherein the "Kyoho hybrid line" is TsuVv001 of type AB, TsuVv002 of type C, TsuVv003 of type AB, TsuVv013 of type AB, TsuVv014 of type C, TsuVv016 of type B, TsuVv019 of type BC, TsuVv025 of type AB, TsuVv030 of type AC, TsuVv031 of type B, TsuVv047 of type AB, and VrZAG83 of type EF. 8) A method for propagating grape plants, comprising a step of asexually propagating a grape plant produced by the production method according to any one of 1) to 7). 9) A method for producing a grape plant, comprising: A breeding step of breeding parents (1) and (2) shown below; Parents (1): Queen Nina, Parents (2): A hybrid line A between Kyoho grapes and a hybrid line B obtained by crossing a Neo Muscat tetraploid grape with Shiramine grapes as the pollen parent. The hybrid line A was crossed with a pollen parent and a seed parent with a hybrid line B. a selection step of selecting candidate grapevine plant lines obtained as a result of the crossbreeding step using genetic markers as indicators, In the selection step, one or more genetic markers selected from the group consisting of TsuVv002, TsuVv019, TsuVv003, TsuVv013, TsuVv014, TsuVv030, and VrZAG83 are used to select candidate grapevine plant lines that have different DNA marker type patterns of the genetic markers from those of the breeding parents (1) and (2). Methods for producing grape plants. 10) The method for producing a grapevine plant according to 9), wherein in the selection step, a combination of one or more genetic markers selected from the group consisting of TsuVv002 and TsuVv019 and one or more genetic markers selected from the group consisting of TsuVv003, TsuVv013, TsuVv014, TsuVv030, and VrZAG83 is used to select a candidate grapevine plant line that has a different DNA marker type pattern of the genetic markers from those of the cross parent (1) and the cross parent (2). 11) The production method according to 10), wherein a candidate grapevine plant line is selected in which TsuVv002 is type C, TsuVv019 is type BC, TsuVv003 is type ABC, TsuVv013 is type B, TsuVv014 is type BC, TsuVv030 is type ABC, and VrZAG83 is type CEF. 12) A method for producing a grape plant according to any one of 9) to 11), wherein the candidate grape plant strain selected in the selection process 1) has a red skin color, 2) has an earlier fruit maturity period than Queen Nina, and 3) has good fruit taste. 13) A method for propagating grape plants, comprising a step of asexually propagating a grape plant produced by the production method according to any one of 9) to 12). 14) A grape plant, wherein the DNA marker type of the genetic marker in the grape plant is: TsuVv001 is type AB, TsuVv002 is type C, TsuVv003 is ABC type, TsuVv013 is type B, TsuVv014 is BC type, TsuVv016 is type B, TsuVv019 is type BC, TsuVv025 is type AB, TsuVv030 is ABC type, TsuVv031 is type B, TsuVv047 is type AB, and VrZAG83 is CEF type, The grape plant. 15) The grape plant according to 14), which is a hybrid variety or a descendant thereof, which has a father (pollen parent) of "Queen Nina" and a mother (seed parent) of "a hybrid line obtained by crossing a hybrid line between Kyoho grapes with a hybrid line between a Neo Muscat tetraploid and Shiramine grapes." 16) The grape plant according to 15), wherein the "hybrid line obtained by crossing a hybrid line between Kyoho grapes with a hybrid line between a Neo Muscat tetraploid and Shiramine" is a hybrid line obtained by crossing a "hybrid line between Kyoho grapes" as a pollen parent and a "hybrid line between a Neo Muscat tetraploid and Shiramine" as a seed parent. 17) The grape plant according to 15) or 16), wherein the "hybrid line between Neo Muscat tetraploid and Shiramine" is a hybrid line obtained by crossing Neo Muscat tetraploid as a seed parent with Shiramine as a pollen parent. 18) A grapevine plant according to any one of 15) to 17), wherein the "hybrid line obtained by crossing a hybrid line between Kyoho grapes with a hybrid line between a Neo Muscat tetraploid and Shiramine grapes" is such that TsuVv001 is AB type, TsuVv002 is AC type, TsuVv003 is ABC type, TsuVv013 is B type, TsuVv014 is BC type, TsuVv016 is B type, TsuVv019 is C type, TsuVv025 is AB type, TsuVv030 is ABC type, TsuVv031 is B type, TsuVv047 is AB type, and VrZAG83 is CEF type. 19) A grape plant according to any one of 15) to 18), wherein the "hybrid line between Neo Muscat tetraploid and Shiramine" is such that TsuVv001 is AB type, TsuVv002 is AC type, TsuVv003 is ABC type, TsuVv013 is AB type, TsuVv014 is BC type, TsuVv016 is B type, TsuVv019 is BC type, TsuVv025 is AB type, TsuVv030 is ABC type, TsuVv031 is B type, TsuVv047 is AB type, and VrZAG83 is CE type. 20) A grape plant according to any one of 15) to 19), wherein the "Kyoho hybrid lines" are TsuVv001 AB type, TsuVv002 C type, TsuVv003 AB type, TsuVv013 AB type, TsuVv014 C type, TsuVv016 B type, TsuVv019 BC type, TsuVv025 AB type, TsuVv030 AC type, TsuVv031 B type, TsuVv047 AB type, and VrZAG83 EF type. 21) A novel grape plant, - Obtained by crossing the following breeding parent (1) as a pollen parent and the breeding parent (2) as a seed parent, Parents (1): Queen Nina, Parents (2): A hybrid line A between Kyoho grapes and a hybrid line B obtained by crossing a Neo Muscat tetraploid grape with Shiramine grapes as the pollen parent. The hybrid line A was crossed with a pollen parent and a seed parent with a hybrid line B. -The skin color is red, -The fruit ripens earlier than Queen Nina. - The fruit has good eating quality, and - distinguishable from other grape varieties, including the parents (1) and (2), using the genetic markers TsuVv001, TsuVv002, TsuVv003, TsuVv013, TsuVv014, TsuVv016, TsuVv019, TsuVv025, TsuVv030, TsuVv031, TsuVv047, and VrZAG83; Grape plant. 22) A grape plant according to 21), wherein the sugar content (%) of the fruit is 18% or more and the acidity (g / 100ml) of the fruit is 0.4g / 100ml or less. 23) The grape plant according to 21) or 22), wherein TsuVv001 is type AB, TsuVv002 is type C, TsuVv003 is type ABC, TsuVv013 is type B, TsuVv014 is type BC, TsuVv016 is type B, TsuVv019 is type BC, TsuVv025 is type AB, TsuVv030 is type ABC, TsuVv031 is type B, TsuVv047 is type AB, and VrZAG83 is type CEF. 24) A grape plant according to any one of 14) to 23), in the form of a seedling or scion. 25) Fruit of a grape plant described in any one of 14) to 24). 26) The fruit of the grape plant according to 25), which is seedless. 27) A processed product of the fruit of the grape plant according to 25) or 26). 28) A packaged product comprising the fruit of the grape plant according to 25) or 26) packaged in a packaging material for packaging fruit.

[0036] 2-1) A method for producing a grape plant, comprising a step of crossbreeding the following crossbreeding parents (1) and (2): crossbreeding parent (1): Queen Nina; crossbreeding parent (2): a hybrid line obtained by crossbreeding a hybrid line between Kyoho grapes with a hybrid line between a Neo Muscat tetraploid and Shiramine grapes; The method for producing grape plants includes a selection step in which candidate grape plant lines obtained as a result of the crossbreeding step are selected using genetic markers as indicators, in which one or more genetic markers selected from the group consisting of TsuVv002, TsuVv019, TsuVv003, TsuVv013, TsuVv014, TsuVv030, and VrZAG83 are used to select candidate grape plant lines having different DNA marker type patterns of the genetic markers from those of the crossbreeding parents (1) and (2). 2-2) The production method described in 2-1), wherein in the selection step, a combination of one or more genetic markers selected from the group consisting of TsuVv002 and TsuVv019 and one or more genetic markers selected from the group consisting of TsuVv003, TsuVv013, TsuVv014, TsuVv030, and VrZAG83 is used to select candidate grape plant lines that have different DNA marker type patterns of the genetic markers from those of the breeding parents (1) and (2). 2-3) A method for producing a candidate grapevine plant strain according to 2-2), in which TsuVv002 is type C, TsuVv019 is type BC, TsuVv003 is type ABC, TsuVv013 is type B, TsuVv014 is type BC, TsuVv030 is type ABC, and VrZAG83 is type CEF, is selected. 2-4) A method for producing a grape plant according to any one of 2-1) to 2-3), wherein the candidate grape plant strain selected in the selection process has a red skin color, has an earlier fruit maturity period than Queen Nina, and has good fruit taste. 2-5) A method for propagating grape plants, comprising a step of asexually propagating grape plants produced by the production method described in any one of 2-1) to 2-4) above. 2-6) A novel grape plant, - Obtained by crossing the following breeding parents (1) and (2): Breeding parent (1): Queen Nina; Breeding parent (2): A hybrid line obtained by crossing a hybrid line between Kyoho grapes with a hybrid line between a Neo Muscat tetraploid and Shiramine grapes; -The skin color is red, -The fruit ripens earlier than Queen Nina. - The fruit has good eating quality, and - distinguishable from other grapevine varieties, including the breeding parents (1) and (2), using one or more genetic markers selected from the group consisting of TsuVv001, TsuVv002, TsuVv003, TsuVv013, TsuVv014, TsuVv016, TsuVv019, TsuVv025, TsuVv030, TsuVv031, TsuVv047, and VrZAG83; Grape plant. 2-7) A grape plant according to 2-6), in which the sugar content (%) of the fruit is 18% or more and the acidity (g / 100ml) of the fruit is 0.4g / 100ml or less. 2-8) A grape plant according to 2-6) or 2-7), wherein TsuVv001 is type AB, TsuVv002 is type C, TsuVv003 is type ABC, TsuVv013 is type B, TsuVv014 is type BC, TsuVv016 is type B, TsuVv019 is type BC, TsuVv025 is type AB, TsuVv030 is type ABC, TsuVv031 is type B, TsuVv047 is type AB, and VrZAG83 is type CEF. 2-9) A grape plant according to any one of 2-6) to 2-8), which is in the form of a seedling or scion. 2-10) The fruit of a grape plant according to any one of 2-6) to 2-9), which is seedless. 2-11) A packaged product comprising the fruit of the grape plant according to any one of 2-6) to 2-10) above packaged in a packaging material for packaging fruit. 2-12) A method for identifying a grape plant according to any one of 2-6) to 2-10) above, comprising a step of determining the DNA marker type of one or more genetic markers selected from the group consisting of TsuVv001, TsuVv002, TsuVv003, TsuVv013, TsuVv014, TsuVv016, TsuVv019, TsuVv025, TsuVv030, TsuVv031, TsuVv047, and VrZAG83. The present invention is not limited to the above-described embodiments, and various modifications are possible within the scope of the claims. Embodiments obtained by appropriately combining the technical means disclosed in different embodiments are also included in the technical scope of the present invention. [Example]

[0037] Example 1: Development of a novel grape plant At the Persimmon and Grape Branch of the Fruit Tree Research Station (currently the Grape and Persimmon Research Center of the Fruit Tree and Tea Research Department of the National Agriculture and Food Research Organization), a new grape plant, "Grape AK31," was developed using the hybrid breeding method outlined below. The breeding pedigree of "Grape AK31" is shown in Figure 1. (1) Year 1: Queen Nina was crossed with grape hybrid line 396-56 (Akitsu 22 (seed parent) x Akitsu 14 (pollen parent)), and the resulting seeds were harvested. In this example, Queen Nina was used as the pollen parent, and grape hybrid line 396-56 was used as the seed parent. Akitsu 22 is a hybrid line obtained by crossing Neo Muscat (tetraploid) (seed parent) with Shiramine (pollen parent), and Akitsu 14 is a hybrid line obtained by crossing Kyoho grapes. (2) Second year: The seeds obtained in (1) were sown in a greenhouse. Since the seedlings did not grow well after germination, they were grown as self-rooted seedlings until the following year. (3) Third year: Seedlings were grown by green grafting onto the rootstock "Teleki 5BB". (4) From the fourth year onwards: The seedlings obtained in (3) were planted in the selected field. Cultivation continued in the same field for the fifth year. (5) 6th year: This individual was selected as a noteworthy specimen due to its first fruiting and good taste. It also had other notable characteristics, such as early maturity, no cracking, firm flesh, and a pleasant fragrance. (6) From the 7th year: We investigated the use of gibberellin treatment to make the plants seedless, and continued to investigate their characteristics. (7) Year 8: Further investigation was conducted into the possibility of seedlessness through gibberellin treatment. Primary selection was conducted with a focus on early-ripening varieties with red skin, strong sweetness (high sugar content), low acidity, good taste, and good seed removal. The high sugar content (%) criteria were 19.0% or higher, and the high acidity (g / 100ml) criteria were 0.4 (g / 100ml) or lower. (8) From the 9th year: The line number "Grape AK31" was assigned, and the line was used in a line adaptability test, and its characteristics were examined throughout Japan.

[0038] Example 2: Main characteristics of the new grape plant (1) The characteristics of the novel grape plant "Grape AK31" obtained in Example 1 are shown in Table 5 in comparison with those of "Aki Queen" and "Kyoho."

[0039] [Table 5]

[0040] (Method of investigating characteristics) The survey was conducted annually for three years from 2021 to 2023, and the figures are expressed as the average value over the three years. In addition, streptomycin was sprayed before flowering, and gibberellin treatment (25 ppm) was performed twice, at full flowering and 10 to 15 days after full flowering, to obtain seedless fruits for evaluation. Figure 2 shows a full view of the fruiting state of "Grape AK31," and Figure 3 shows the clusters of "AK31." Figures 2 and 3 show clusters that were not treated with gibberellin for reference. Tree vigor was assessed on a three-point scale from "weak" to "strong" by observing the strength of new shoot growth and the thickness and length of branches, with "Delaware" being "medium" and "Kyoho," "Neo Muscat," and "Koshu" being "strong." The flowering period was measured on the day when 80% of the flower spikes reached 80% of the total (peak flowering period). Flower shedding was assessed on a three-point scale by comparing the number of flower buds on the formed inflorescences with the number of seed-bearing fruit. Delaware and Steuben were assessed as "low," Kyoho as "medium," and Pione and Aki Queen as "high." The harvest period was determined as the day when 50% or more of the fruits with excellent fruit quality were harvested (peak harvest period). The cluster weight was the average value of 10 formed clusters, and the average weight of each fruit (berry weight) was determined by measuring 10 berries per formed cluster and evaluating the average value of each berry. The flower spikes were shaped from about 3.5 cm below the spike so that about 38 berries would be set on a 7 cm spike. - Skin color was judged as "yellow-green or yellow-white" for "Neo Muscat," "pale pink (pale red)" for "Koshu," "red" for "Ruby Okuyama" and "Aki Queen," "reddish-brown" for "Delaware" and "North Red," "purple-red" for "Oriental Star," and "purple-black or blue-black" for "Kyoho" and "Campbell Early." The difficulty of peeling was assessed based on the ability to separate the skin and flesh when eaten, on a three-point scale: "easy," "medium," and "difficult." The peeling was compared with the following varieties: "Easy": Delaware, "Campbell Early," "Medium": Kyoho, "Pione," "Difficult": Kaiji, "Risamato." The characteristics of the flesh were evaluated sensorily based on how easily the flesh broke apart when eaten. They were divided into three categories: "crumbly," "intermediate," and "lumpy." These were compared with the following varieties: "Risamato" and "Shine Muscat" for "crumbly," "intermediate" for "Kyoho" and "Pione," and "lumpy" for "Delaware" and "Campbell Early." -Flesh firmness was evaluated sensorily on a three-point scale, with the following varieties being compared: "soft": Niagara, "Ryuho," "medium": Kyoho, "Neo Muscat," "hard": Campbell Early, "Muscat of Alexandria." The sugar content was measured by taking five berries from each bunch and squeezing the juice from the ten bunches at once, and the reading of the juice was measured using a refractometer. The acid content was determined by titrating the juice obtained in the same manner with sodium hydroxide solution, converting the value obtained to tartaric acid, and expressing it as the weight (g) of tartaric acid contained in 100 ml of juice. The aroma was judged by the sense of smell when eating, with "Kaiji" and "Rizamato" compared as "Pear," "Neo Muscat" and "Ruby Okuyama" compared as "Muscat," "Kyoho" and "Campbell Early" compared as "Foxy," and "Himrod" and "Cabernet Sauvignon" compared as "Other." Astringency was assessed by sensory evaluation of the astringency felt when eating, using a four-point scale: "none": no astringency, "slight": slight astringency, "medium": clearly astringent, resulting in poor taste, "high": too astringent to eat. Fruit cracking susceptibility was classified as follows based on the average rate of cracked fruit at maturity for five shaped fruit clusters: "none": no cracked fruit, "very few": 0-5% cracked fruit, "few": 5-10%, "medium": 10-20%, "many": 20-50%, and "very many": 50% or more. - Shedding resistance was evaluated on a three-point scale based on the resistance of the berries when they were separated from the stalk, in comparison with the following varieties: "Easy": Kyoho and Campbell Early; "Medium": Delaware and Neo Muscat; "Difficult": Kaiji.

[0041] Example 3: Main characteristics of the new grape plant (2) Tables 6 and 7 show the characteristics of the novel grape plant "Grape AK31" obtained in Example 1, compared with those of "Aki Queen" and "Kyoho." The survey was based on data from three years from 2021 to 2023, and the values ​​are expressed as average values ​​over the three years. In addition, streptomycin was sprayed before flowering, and gibberellin treatment (25 ppm) was performed twice, at full bloom and 10 to 15 days after full bloom, to obtain seedless fruit for evaluation. Other evaluation conditions were the same as those described in Example 2.

[0042] [Table 6]

[0043] [Table 7]

[0044] Tables 8 and 9 compare the characteristics of the novel grape plant "Grape AK31" obtained in Example 1 with those of "Queen Nina." The characteristics of "Grape AK31" are the same as those in Table 2 and are based on data from three years (2021 to 2023), with the values ​​expressed as averages over the three years. The characteristics of "Queen Nina" are based on data from one year (2023). In addition, streptomycin was sprayed before flowering, and gibberellin treatment (25 ppm) was performed twice, once at full bloom and once 10 to 15 days after full bloom, to obtain seedless fruit for evaluation. Other evaluation conditions were the same as those described in Example 2. Regarding the characteristics of grape hybrid line 396-56, distinct differences from those of "Grape AK31" include a yellow-green skin color, a muscat-like aroma, slightly soft flesh, intermediate flesh characteristics, and a smaller cluster weight. Data on sugar content and acid content were not obtained.

[0045] [Table 8]

[0046] [Table 9]

[0047] Example 4: Variety identification using known genetic markers DNA samples were extracted from grape skins of the novel grape plant "Grape AK31" obtained in Example 1 and its hybrid parents, "Queen Nina" and "Grape hybrid line 396-56," and the DNA marker type patterns of 12 known SSR markers (TsuVv001, TsuVv002, TsuVv003, TsuVv013, TsuVv014, TsuVv016, TsuVv019, TsuVv025, TsuVv030, TsuVv031, TsuVv047, and VrZAG83) were determined. The DNA marker type patterns of SSR markers were determined according to the method described in the above-mentioned literature (3) “DNA variety identification technology for 24 grape varieties - DNA variety identification technology for 24 grape varieties using SSR markers - (National Agriculture and Food Research Organization) / First edition January 27, 2021 / National Agriculture and Food Research Organization, Fruit Tree and Tea Industry Research Division.” The results are shown in Tables 3 and 4. As the results show, the DNA marker type patterns of SSR markers for "Grape AK31," "Queen Nina," and "Grape Hybrid 396-56" are different from each other, indicating that cultivars can be distinguished. Furthermore, it was shown that cultivars of "Grape AK31," "Queen Nina," and "Grape Hybrid 396-56" can be distinguished from the 28 major cultivated cultivars shown in these results. [Industrial Applicability]

[0048] The present invention can be used, for example, in the field of agriculture.

Claims

1. 1. A method for producing a grapevine plant, comprising: The father (pollen parent) is "Queen Nina" and the mother (seed parent) is "a hybrid line obtained by crossing a hybrid line between Kyoho grapes and a hybrid line between a Neo Muscat tetraploid and Shiramine grapes." Hybrid varieties obtained by crossbreeding or their progeny plants: (Here, the "hybrid line obtained by crossing a hybrid line between Kyoho grapes with a hybrid line between a Neo Muscat tetraploid and Shiramine" is a hybrid line obtained by crossing a "hybrid line between Kyoho grapes" as a pollen parent and a "hybrid line between a Neo Muscat tetraploid and Shiramine" as a seed parent, and TsuVv001 is AB type, TsuVv002 is AC type, TsuVv003 is ABC type, TsuVv013 is B type, TsuVv014 is BC type, TsuVv016 is B type, TsuVv019 is C type, TsuVv025 is AB type, TsuVv030 is ABC type, TsuVv031 is B type, TsuVv047 is AB type, and VrZAG83 is CEF type. The "Kyoho inter-hybrid line" is one in which TsuVv001 is AB type, TsuVv002 is C type, TsuVv003 is AB type, TsuVv013 is AB type, TsuVv014 is C type, TsuVv016 is B type, TsuVv019 is BC type, TsuVv025 is AB type, TsuVv030 is AC type, TsuVv031 is B type, TsuVv047 is AB type, and VrZAG83 is EF type; and The "hybrid line between Neo Muscat tetraploid and Shiramine" is a hybrid line obtained by crossing Neo Muscat tetraploid as a seed parent with Shiramine as a pollen parent, in which TsuVv001 is AB type, TsuVv002 is AC type, TsuVv003 is ABC type, TsuVv013 is AB type, TsuVv014 is BC type, TsuVv016 is B type, TsuVv019 is BC type, TsuVv025 is AB type, TsuVv030 is ABC type, TsuVv031 is B type, TsuVv047 is AB type, and VrZAG83 is CE type. The following genetic markers TsuVv001, TsuVv002, TsuVv003, TsuVv013, TsuVv014, TsuVv016, TsuVv019, TsuVv025, TsuVv030, TsuVv031, TsuVv047, and VrZAG83 were identified for TsuVv001 is type AB, TsuVv002 is type C, TsuVv003 is ABC type, TsuVv013 is type B, TsuVv014 is BC type, TsuVv016 is type B, TsuVv019 is type BC, TsuVv025 is type AB, TsuVv030 is ABC type, TsuVv031 is type B, TsuVv047 is type AB, and VrZAG83 is CEF type A method for producing grape plants, comprising the step of selecting plants having a DNA marker type.

2. A method for propagating grape plants, comprising a step of asexually propagating grape plants produced by the production method of claim 1.

3. 1. A method for producing a grapevine plant, comprising: A cross-breeding step of cross-breeding parents (1) and (2) shown below; Breeding parents (1): Queen Nina, Parent (2): A hybrid line A between Kyoho grapes and a hybrid line B obtained by crossing a Neo Muscat tetraploid grape with Shiramine grapes as the pollen parent. The hybrid line A was crossed with a pollen parent and a seed parent with a hybrid line B. (Here, the "hybrid line obtained by crossing a hybrid line between Kyoho grapes with a hybrid line between a Neo Muscat tetraploid and Shiramine" is a hybrid line obtained by crossing a "hybrid line between Kyoho grapes" as a pollen parent and a "hybrid line between a Neo Muscat tetraploid and Shiramine" as a seed parent, and TsuVv001 is AB type, TsuVv002 is AC type, TsuVv003 is ABC type, TsuVv013 is B type, TsuVv014 is BC type, TsuVv016 is B type, TsuVv019 is C type, TsuVv025 is AB type, TsuVv030 is ABC type, TsuVv031 is B type, TsuVv047 is AB type, and VrZAG83 is CEF type. The "Kyoho inter-hybrid line" is one in which TsuVv001 is AB type, TsuVv002 is C type, TsuVv003 is AB type, TsuVv013 is AB type, TsuVv014 is C type, TsuVv016 is B type, TsuVv019 is BC type, TsuVv025 is AB type, TsuVv030 is AC type, TsuVv031 is B type, TsuVv047 is AB type, and VrZAG83 is EF type; and The "hybrid line between Neo Muscat tetraploid and Shiramine" is a hybrid line obtained by crossing Neo Muscat tetraploid as a seed parent with Shiramine as a pollen parent, in which TsuVv001 is AB type, TsuVv002 is AC type, TsuVv003 is ABC type, TsuVv013 is AB type, TsuVv014 is BC type, TsuVv016 is B type, TsuVv019 is BC type, TsuVv025 is AB type, TsuVv030 is ABC type, TsuVv031 is B type, TsuVv047 is AB type, and VrZAG83 is CE type. a selection step of selecting candidate grapevine plant lines obtained as a result of the crossbreeding step using genetic markers as indicators, In the selection step, one or more genetic markers selected from the group consisting of TsuVv002, TsuVv019, TsuVv003, TsuVv013, TsuVv014, TsuVv030, and VrZAG83 are used to select candidate grapevine plant lines that have different DNA marker type patterns of the genetic markers from those of the breeding parents (1) and (2). Methods for producing grape plants.

4. The production method described in claim 3, wherein in the selection process, a combination of one or more genetic markers selected from the group consisting of TsuVv002 and TsuVv019 and one or more genetic markers selected from the group consisting of TsuVv003, TsuVv013, TsuVv014, TsuVv030, and VrZAG83 is used to select candidate grape plant lines that have different DNA marker type patterns of the genetic markers from those of the breeding parents (1) and (2).

5. The above TsuVv002 is type C, the above TsuVv019 is type BC, the above TsuVv003 is type ABC, the above TsuVv013 is type B, the above TsuVv014 is type BC, the above TsuVv030 is type ABC, and the above VrZAG83 The method for producing a candidate grape plant according to claim 4, wherein a line of the candidate grape plant is of the CEF type.

6. The method for producing a grape plant according to any one of claims 3 to 5, wherein the candidate grape plant strain selected in the selection process 1) has a red skin color, 2) has an earlier fruit maturity period than Queen Nina, and 3) has good fruit taste.

7. A method for propagating grape plants, comprising a step of asexually propagating grape plants produced by the production method according to any one of claims 3 to 5.

8. The father (pollen parent) is "Queen Nina" and the mother (seed parent) is a hybrid variety or its descendants obtained by crossing a hybrid line between Kyoho grapes and a hybrid line between Neo Muscat tetraploid and Shiramine grapes. (Here, the "hybrid line obtained by crossing a hybrid line between Kyoho grapes with a hybrid line between a Neo Muscat tetraploid and Shiramine" is a hybrid line obtained by crossing a "hybrid line between Kyoho grapes" as a pollen parent and a "hybrid line between a Neo Muscat tetraploid and Shiramine" as a seed parent, and TsuVv001 is AB type, TsuVv002 is AC type, TsuVv003 is ABC type, TsuVv013 is B type, TsuVv014 is BC type, TsuVv016 is B type, TsuVv019 is C type, TsuVv025 is AB type, TsuVv030 is ABC type, TsuVv031 is B type, TsuVv047 is AB type, and VrZAG83 is CEF type. The "Kyoho inter-hybrid line" is one in which TsuVv001 is AB type, TsuVv002 is C type, TsuVv003 is AB type, TsuVv013 is AB type, TsuVv014 is C type, TsuVv016 is B type, TsuVv019 is BC type, TsuVv025 is AB type, TsuVv030 is AC type, TsuVv031 is B type, TsuVv047 is AB type, and VrZAG83 is EF type; and The "hybrid line between Neo Muscat tetraploid and Shiramine" is a hybrid line obtained by crossing Neo Muscat tetraploid as a seed parent with Shiramine as a pollen parent, in which TsuVv001 is AB type, TsuVv002 is AC type, TsuVv003 is ABC type, TsuVv013 is AB type, TsuVv014 is BC type, TsuVv016 is B type, TsuVv019 is BC type, TsuVv025 is AB type, TsuVv030 is ABC type, TsuVv031 is B type, TsuVv047 is AB type, and VrZAG83 is CE type. A grape plant, wherein the DNA marker type of a genetic marker in said grape plant is: TsuVv001 is type AB, TsuVv002 is type C, TsuVv003 is ABC type, TsuVv013 is type B, TsuVv014 is BC type, TsuVv016 is type B, TsuVv019 is type BC, TsuVv025 is type AB, TsuVv030 is ABC type, TsuVv031 is type B, TsuVv047 is type AB, and VrZAG83 is CEF type, The grape plant.

9. The father (pollen parent) is "Queen Nina" and the mother (seed parent) is a hybrid line obtained by crossing a Kyoho cross with a Neo Muscat tetraploid and a Shiramine cross. (Here, the "hybrid line obtained by crossing a hybrid line between Kyoho grapes with a hybrid line between a Neo Muscat tetraploid and Shiramine" is a hybrid line obtained by crossing a "hybrid line between Kyoho grapes" as a pollen parent and a "hybrid line between a Neo Muscat tetraploid and Shiramine" as a seed parent, and TsuVv001 is AB type, TsuVv002 is AC type, TsuVv003 is ABC type, TsuVv013 is B type, TsuVv014 is BC type, TsuVv016 is B type, TsuVv019 is C type, TsuVv025 is AB type, TsuVv030 is ABC type, TsuVv031 is B type, TsuVv047 is AB type, and VrZAG83 is CEF type. The "Kyoho inter-hybrid line" is one in which TsuVv001 is AB type, TsuVv002 is C type, TsuVv003 is AB type, TsuVv013 is AB type, TsuVv014 is C type, TsuVv016 is B type, TsuVv019 is BC type, TsuVv025 is AB type, TsuVv030 is AC type, TsuVv031 is B type, TsuVv047 is AB type, and VrZAG83 is EF type; and The "hybrid line between Neo Muscat tetraploid and Shiramine" is a hybrid line obtained by crossing Neo Muscat tetraploid as a seed parent with Shiramine as a pollen parent, in which TsuVv001 is AB type, TsuVv002 is AC type, TsuVv003 is ABC type, TsuVv013 is AB type, TsuVv014 is BC type, TsuVv016 is B type, TsuVv019 is BC type, TsuVv025 is AB type, TsuVv030 is ABC type, TsuVv031 is B type, TsuVv047 is AB type, and VrZAG83 is CE type. A grape plant, wherein the DNA marker type of a genetic marker in said grape plant is: TsuVv001 is type AB, TsuVv002 is type C, TsuVv003 is ABC type, TsuVv013 is type B, TsuVv014 is BC type, TsuVv016 is type B, TsuVv019 is type BC, TsuVv025 is type AB, TsuVv030 is ABC type, TsuVv031 is type B, TsuVv047 is type AB, and VrZAG83 is CEF type, -The skin color is red, -The fruit ripens earlier than Queen Nina. - The fruit has good eating quality, and - distinguishable from other grape varieties, including the parents (1) and (2), using the genetic markers TsuVv001, TsuVv002, TsuVv003, TsuVv013, TsuVv014, TsuVv016, TsuVv019, TsuVv025, TsuVv030, TsuVv031, TsuVv047, and VrZAG83; Grape plant.

10. 10. The grape plant according to claim 9, wherein the sugar content (%) of the fruit is 18% or more and the acidity (g / 100 ml) of the fruit is 0.4 g / 100 ml or less.

11. 10. A grape plant according to claim 8 or 9 in the form of a seedling or scion.

12. Fruit of a grape plant according to claim 8 or 9.

13. 13. The fruit of a grape plant according to claim 12, which is a seedless fruit.

14. A processed product of the fruit of the grape plant according to claim 12.

15. A packaged product, comprising the fruit of the grape plant according to claim 12 packaged in a packaging material for packaging fruit.

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