Use of Moringa oleifera seed protein extract as an anti-pollen agent

Abstract

The present invention relates to the use of a protein extract of ungerminated, de-oiled seeds of Moringa oleifera as an anti-pollinator.The present invention further relates to a protein extract of ungerminated, de-oiled seeds of Moringa oleifera for use in the treatment and / or prevention and / or reduction of the manifestations of pollen allergy and / or the symptoms and / or signs associated with pollen allergy.

Description

Technical Field

[0001] The present invention relates to household hygiene products, particularly in the fields of cosmetics and dermatology. The present invention aims at the use of a protein extract from non-germinated defatted seeds of Moringa as a pollen inhibitor and a pharmaceutical, particularly a skin product, which prevents and / or reduces pollen allergy and the onset of its pathological conditions.

Background Art

[0002] The genus Moringa contains about 14 species of plants (among which, particularly Moringa peregrina, M. aptera, M. concanensis, M. drouhardii, M. hildebrandtii, M. longituba are included), and among them, Moringa pterygosperma (synonymous with Moringa oleifera) is the most well-known.

[0003] The genus Moringa refers to trees that grow quickly, adapt very well to various conditions, and grow throughout the tropics, in Asia, Africa, and South America. The fruit, which is 30 - 50 cm long, hangs like a percussion instrument mallet, which is the origin of the English name "drumstick tree", and the green pods are highly evaluated as vegetables around the world. Therefore, it is rare for the seeds to be left until they mature for oil production.

[0004] [[ID=第十九]] Various parts of the tree (leaves, roots, root bark, flowers, seeds) are used in traditional medicine in the countries where the genus Moringa grows.

[0005] Moringa seeds are characterized by having an oil with a content of 21 - 53% depending on the species and seed maturity. For Moringa oleifera seeds, the content described in the literature ranges from 21 - 34%.

[0006] Due to its excellent oxidative stability and good fragrance-fixing properties, moringa oil, also known as behen oil or ben oil, was the most widely used oil by ointment compounders in ancient civilizations for cosmetic and religious purposes. This oil was used by compounders as a cosmetic until the last century, and its use has recently been "rediscovered."

[0007] Aside from its oil content, moringa seeds have more recently attracted the attention of researchers for their protein extract. Accordingly, European Patent Application No. 1064008 describes the use of moringa seed protein extract on the skin and mucous membranes for its soothing, conditioning, moisturizing, reconstructive, restorative, anti-wrinkle, and anti-fouling effects.

[0008] Such extracts are specifically marketed by BASF under the names Purisoft® (registered trademark) for their purifying and anti-pollution properties against particulate matter, and Puricare® (registered trademark) for their hair-protecting properties against pollution.

[0009] International Patent Application No. 02 / 096448 describes the use of defatted or undefatted seed protein extracts in the fields of deodorant, odor removal, cleanliness, household hygiene, oral hygiene, and dental care.

[0010] More recently, French Patent Application No. 3 076 460 also describes the use of moringa seed protein extract to prevent and / or reduce and / or eliminate unsightly and / or bothersome effects of altered skin and / or mucous membranes, specifically sebum secretion, and to prevent and / or reduce and / or delay unsightly and / or unpleasant and / or bothersome signs, specifically to prevent and / or reduce and / or delay comedogenesis and / or oiliness of the skin, and / or to maintain and / or improve the uniformity of skin and / or mucous membrane color by, for example, removing and / or reducing redness and / or unevenness, and / or to prevent and / or treat hot and / or burning sensations of the skin and / or mucous membranes, and / or to prevent and / or reduce hair and / or hair loss, and / or to prevent and / or reduce dandruff. [Overview of the project] [Problems that the invention aims to solve]

[0011] However, none of these studies describe or suggest that moringa seed protein extracts may help protect surfaces, more specifically the skin, mucous membranes, and / or skin appendages, from pollen.

[0012] Therefore, the present invention relates to a novel use of protein extract from ungerminated, defatted seeds of Moringa oleifera, which is neither described nor suggested in the prior art.

[0013] In fact, the inventors have found that such extracts can prevent pollen grains from adhering to the skin, mucous membranes, and / or skin appendages, and more generally, to any type of surface (Example 2). Therefore, this extract has a very interesting anti-pollen activity and thus corresponds to a product that can be selected for surface treatment, specifically skin and / or mucous membranes and / or hair exposed to pollen, and specifically in people who are hypersensitive to and / or allergic to pollen.

[0014] Pollen is an extremely fine dust composed of tiny organic particles produced and released by the anthers of plants. Pollen grains are more or less oval-shaped, ranging from 5 to 200 microns in size, with an average size of 20 to 60 microns.

[0015] Pollen grains are highly complex aggregates characterized by a rather specialized organic structure. A hard outer layer, which serves a protective function, is called the outer membrane. The outer membrane has a different appearance depending on the species, allowing plant species to be characterized simply by observing the pollen grain. The outer membrane is covered with ridges and depressions filled with a nearly lipid-based substance called the pollen coat. The former is sticky, giving the pollen its special adhesion properties. Thus, this adhesion is ensured by the decoration of the outer membrane and / or the viscous lipid coating that covers it, and therefore adheres to animals that mediate pollination.

[0016] However, pollen is a cause of health problems on a global scale. In Asia, cedar pollen is described as a genuine seasonal hazard. In 2019, an increase in cedar pollen allergies was reported. Cedar pollen hypersensitivity was detected in 78% of Japanese people, and of those, 41% reported pollen-related complaints. Similar problems have been reported in North America with mountain cedar. In Europe, ragweed pollen, which is highly allergenic, could quadruple around 2050. In France, ragweed (Ambrosia artemisiifolia), which is native to North America, is already established in Burgundy, Auvergne, and the Rhône-Alpes region, and its pollen causes allergies that manifest particularly as lesions, such as rhinitis, sinusitis, conjunctivitis, and tracheitis, and can even trigger asthma attacks. These problems also have a dramatic impact on the skin, as pollen can trigger skin allergic inflammation characterized by dryness, redness, and skin rashes. Furthermore, exposure to pollen can worsen eczema.

[0017] Pollen-induced damage is exacerbated by urban pollutants. In fact, studies have shown that air pollutants, specifically fine particulate matter, can enhance the amount of allergens present in pollen grains and their ability to be released into the atmosphere, releasing allergenic sites and thus increasing their allergenicity (D'amato G et al, 2007: allergic pollen and pollen allergy in Europe; Laaidi M, 2011: pollen allergies, pollution and climate: Literature review; Sedghy F et al, 2018: Interaction between Air pollutants and pollen grains: the role on the rising trend in allergy).

[0018] Treating pollen allergies often requires the use of antihistamines and / or corticosteroids, which can have numerous side effects. Preventing exposure to pollen, the cause of allergies, remains a crucial step towards avoiding reliance on allergy treatment. However, the health effects of pollen are not limited to outdoor exposure to pollination areas; they also exist indoors. In fact, pollen grains adhere to virtually any surface, such as fabrics, especially clothing and shoes, as well as skin, mucous membranes, and skin appendages, specifically hair fibers. Therefore, pollen grains can adhere to these surfaces and then be released again indoors. This phenomenon is particularly common with long hair.

[0019] Therefore, in the fields of household hygiene, and more specifically in cosmetics and dermatology, there is a growing need to provide readily available ingredients that limit and / or reduce the amount of pollen generally present on surfaces, more specifically on the skin, mucous membranes, and / or skin appendages, especially hair, by preventing its adhesion. [Means for solving the problem]

[0020] Accordingly, the present invention relates to the use of a protein extract of unsprouted, defatted seeds of Moringa oleifera, preferably a protein extract of unsprouted, defatted seeds, specifically of unsprouted seeds of Moringa oleifera, more preferably a defatted cake of the kernels of the unsprouted seeds, and more specifically a protein extract of the defatted cake, as an anti-pollen agent.

[0021] In the sense of the present invention, "anti-pollen agent" means any compound or mixture of compounds having the property of limiting and / or reducing the amount of pollen present on a surface, specifically the outer surface, more specifically the skin, mucous membranes, and / or skin appendages, specifically the hair, in order to prevent its adhesion and / or its dispersal.

[0022] The property of limiting and / or reducing the amount of pollen present on the surface, specifically the outer surface, and especially on the hair, can be measured by the test shown in Example 2.

[0023] Specifically, the use of the present invention is preferably for the treatment of pollen-exposed skin and / or mucous membranes and / or skin appendages, such as the surface of hair, in people who are hypersensitive to and / or allergic to pollen. Specifically, this is for use as a cosmetic.

[0024] In the sense of this invention, "cosmetic (as)" means use that is not intended for the prevention and / or treatment of lesions on the skin and / or mucous membranes and / or skin appendages, as determined by a specialist in the field, such as a dermatologist, and is not intended as a medicine or a treatment. Therefore, it is used on healthy skin and / or mucous membranes and / or skin appendages.

[0025] "Healthy skin and / or mucosa and / or healthy skin appendages" means the whole or part of the skin area, especially of humans, such as healthy scalp and / or mucosa and / or skin appendages, thus without infection, scar, skin diseases or symptoms, such as candidiasis, impetigo, psoriasis, eczema, acne, ichthyosis, gingivitis or dermatitis, or wounds or traumas, or aphthae or ulcers, or burns, and / or other skin diseases, or aphthosis or inflammation or mild irritation, or urticaria or allergy, such as contact allergy, that is, the whole or part of the skin area does not show such conditions.

[0026] "Skin" in the sense of the present invention means the whole or part of the body, especially of humans, selected from the face, hands, arms, décolleté, legs, neck, back, shoulders, abdomen, wrists, forearms, ankles, calves, nape of the neck, such as the scalp, etc. Specifically, the skin is what may come into contact with pollen, that is, it is not covered by clothing. Thus, more specifically, it is the face, hands, neck, and décolleté.

[0027] "Mucosa" in the sense of the present invention means ocular mucosa, nasal mucosa, and / or oral mucosa, especially oral labial mucosa, preferably labial mucosa, ocular mucosa, and / or nasal mucosa.

[0028] "Skin appendages" means nails and "keratin fibers".

[0029] "Keratin fibers" means hair fibers (hairs), eyelashes, eyebrows, especially nasal hair, ear hair, beard, and / or mustache.

[0030] Preferably, the application is local, advantageously on a specific part and / or area of the body exposed to pollen, specifically selected from the face, hands, arms, décolleté, labial mucosa, nasal mucosa, and / or ocular mucosa, body hair, eyelashes, eyebrows, and / or hair, preferably for hair.

[0031] The protein extract of unsprouted, de-oiled seeds of Moringa oleifera according to the present invention is topically acceptable. In the sense of the present invention, "topically acceptable" means an ingredient that is non-toxic, non-inflammatory, suitable for topical application to the skin and / or mucous membranes and / or skin appendages, does not cause allergic reactions, and is not chemically unstable.

[0032] In the sense of the present invention, "pollen" means plant pollen grains, preferably having an average equatorial diameter (E) in the range of 20 to 60 microns and / or an average polar axis (P) in the range of 20 to 60 microns.

[0033] Preferably, the pollen is selected from the pollen of the plants that are most likely to cause allergies, specifically from the following: - Betulaceae family, specifically the genus Betura, especially birch; the genus Aulnus, especially alder; the genus Corylus, especially European hazelnut. - Cupressaceae family, specifically cypress (Cupressus) and Japanese cedar (Cryptomeria japonica), mountain cedar (Mulange Cypress), - Pinaceae family, specifically the Himalayan cedar, - Asteraceae family, specifically ragweed (Ambrosia artemisiifolia), - Grasses (Poaceae) - Quercus serrata and / or Platanus sycamore

[0034] Preferably, the pollen is from birch, hazel, cedar, mountain cedar, rice, and / or ragweed.

[0035] In the present invention, the use of the protein extract of unsprouted, defatted seeds of Moringa oleifera according to the present invention is not for improving the skin's barrier function, nor for enhancing the moisturizing properties of the skin and / or mucous membranes, nor for treating dry skin, nor for soothing the skin and / or mucous membranes, specifically dry skin and / or mucous membranes, nor for relieving and / or reducing fatigue of the skin and / or mucous membranes. Specifically, the use of the protein extract of unsprouted, defatted seeds of Moringa oleifera according to the present invention is not for protecting the skin and / or mucous membranes from pollution, more specifically urban pollution (e.g., heavy metals, exhaust fumes, and / or cigarette smoke), pollution-induced damage, nor for / or for purifying the skin and / or mucous membranes by removing polluting particles, such as coal particles, that cause staining of the skin and / or mucous membranes.

[0036] Furthermore, the use of the present invention is not for the purpose of preventing and / or reducing and / or removing the unsightly and / or bothersome effects of altered commensal bacterial flora on the skin and / or mucous membranes, preventing and / or reducing and / or delaying sebum secretion and unsightly and / or unpleasant and / or bothersome signs, preventing and / or reducing and / or delaying comedone formation and / or comedogenesis and / or oiliness of the skin, and / or maintaining and / or improving the uniformity of the color of the skin and / or mucous membranes by removing and / or reducing redness and / or unevenness, and / or preventing and / or treating hot and / or burning sensations of the skin and / or mucous membranes, and / or preventing and / or reducing hair and / or body hair loss, and / or preventing and / or reducing dandruff.

[0037] In other alternative embodiments, a protein extract of ungerminated, de-oiled seeds of Moringa oleifera is applied to inert surfaces exposed to pollen, particularly surfaces of household items, fibers, especially cloth, such as clothing and / or household fabrics.

[0038] In the sense of the present invention, "local route" means applying (applying) the protein extract and / or composition and / or components of the ungerminated, oil-free seeds of Moringa oleifera according to the present invention to the skin and / or mucous membranes, specifically the external surface, particularly by direct application or vaporization.

[0039] In the sense of this invention, "cosmetic and / or pharmaceutical ingredient" means one or more plant extracts, and / or natural and / or synthetic molecules, and / or mixtures thereof, intended for application (topical application) to cosmetics and / or pharmaceuticals. Cosmetic ingredients are defined in particular by the International Nomenclature for Cosmetic Ingredients (INCI).

[0040] In the context of this invention, the term "suitable cosmetic or pharmaceutical medium" means that the composition or components are suitable for use in contact with human skin and / or mucous membranes, and are free from undesirable toxicity, contraindications, instability, allergic reactions, or equivalent.

[0041] The extracts according to the present invention can be obtained by various extraction methods known to those skilled in the art, which are preferably selected from maceration with or without stirring, heat extraction, crushing, such as ultrasonic crushing or crushing using a mixer. Preferably, the extraction is carried out by maceration, more preferably maceration with stirring.

[0042] Extraction can be carried out at a temperature of 4°C to 300°C, preferably 20°C to 80°C, where 20°C corresponds to the ambient temperature, and is advantageously between 20°C and 25°C.

[0043] The extraction is carried out over a period of 30 minutes to 12 hours, preferably 1 hour to 5 hours, and more preferably 1 hour to 2 hours. Very advantageously, the extraction is carried out over a period of 1 hour.

[0044] The extract according to the present invention is obtained by extraction with a practical polar solvent, preferably water as a single solvent, selected from the group consisting of water, alcohol, glycol, polyol, 99 / 1 to 1 / 99 (w / w) water / alcohol mixture, 99 / 1 to 1 / 99 (w / w) water / glycol mixture, and 99 / 1 to 1 / 99 (w / w) water / polyol mixture (for example, a mixture of water with ethanol, glycerol and / or butylene glycol and / or other glycols, such as xylitol and / or propanediol).

[0045] Specifically, the extract is obtained by aqueous extraction.

[0046] In the sense of the present invention, "extract obtained by aqueous extraction" means any extract obtained by extraction using an aqueous solution containing more than 60% by weight, preferably at least 70% by weight, specifically at least 80% by weight, more specifically at least 90% by weight, specifically at least 95% by weight, of water relative to the total weight of the aqueous solution, and more preferably free of glycol, specifically free of alcohol, and more specifically free of water.

[0047] The extract is obtained from 0.1% to 20%, preferably 1% to 10%, and more preferably 5% to 10% of the raw or dried, preferably dried, ungerminated defatted seeds of Moringa oleifera plant, relative to the total weight of the ungerminated defatted seeds of the plant and the solvent, and more preferably from 10% by weight of the dried material.

[0048] In one advantageous embodiment, a protein extract from ungerminated, defatted seeds of Moringa oleifera is marketed by BASF under the trade name Purisoft®, as described in European Patent No. 1064008.

[0049] Specifically, this refers to an extract obtained by extraction of a water-soluble extract, more specifically, by extraction in a practical polar solvent, preferably water.

[0050] The extraction method is advantageously described in European Patent No. 1064008 and specifically includes the following steps: a) - Preferably, extraction of oil from Moringa oleifera seeds by using a press or reflux extraction with a nonpolar solvent, such as hexane. b) Extraction of the de-oiled cake or powder obtained in step a), preferably as described in Example 1a, using a practical polar solvent, specifically selected from alcohols, polyols, glycols, water in any proportion and mixtures thereof, preferably an aqueous solvent, i.e., a solvent mainly composed of water, and more preferably water as a single solvent, so as to obtain a crude extract.

[0051] Advantageously, the extraction is carried out at ambient temperature.

[0052] The aqueous solvent may be physiological saline with a pH of various values, more preferably in the range of 4 to 8, or a buffer medium.

[0053] The method may also include an additional step c) after step b), which involves precipitation of the aqueous crude extract obtained in step b) without a change in pH, such as by obtaining a basic pH, specifically above 8, more preferably above 11, using NaOH, and recovery of the obtained precipitate, preferably as described in Example 1b.

[0054] The precipitate is then washed to remove insoluble substances and obtain an extract in the form of a protein concentrate, and then solubilized in water.

[0055] The method may also include an additional decantation step d) after the precipitation step c) to allow for better precipitation of the protein, such as the decantation step d) described in Example 1c. Specifically, this step is carried out at a temperature below ambient temperature, preferably 4°C, and more preferably for at least 6 hours, specifically overnight.

[0056] Instead of steps c) and d), step e) may be performed after step b), which includes contacting the aqueous crude extract obtained in step b) with carboxymethylcellulose for preferably 1 hour, specifically at ambient temperature, to fill the carboxymethylcellulose with the protein extract. This step may then be followed by step f), in which the filled carboxymethylcellulose obtained in step e) is contacted with a saline solution, preferably above pH 7, preferably a NaCl solution having pH 7.5, and then the resulting eluate, which corresponds to a partially purified extract, is recovered.

[0057] The protein extract from ungerminated, de-oil-treated Moringa oleifera seeds is, advantageously, an extract from the hulled seeds, i.e., an extract of only the kernel of the seed (without the outer skin or hull).

[0058] Therefore, the method may include a step of removing the seed husk before step a).

[0059] Finally, the obtained extracts (crude extract, protein concentrate, and / or partially or entirely purified extracts) may be centrifuged and / or filtered and / or distilled to obtain a liquid form after recovering the water-soluble fraction. Preferably, the supernatant obtained after centrifugation is advantageously filtered at a cutoff threshold of 0.45 μm. Additional decolorization and / or deodorization steps may be performed on the extract at any stage of extraction according to techniques known to those skilled in the art. Specifically, the extract may be decolorized with activated carbon.

[0060] In one specific embodiment, the extract according to the present invention is obtained by extracting unsprouted seed cake, specifically de-oiling, more preferably defatted seed cake, in a saline solution with a pH of approximately 5, followed by removal of high molecular weight proteins. The supernatant is collected and constitutes the extract according to the present invention.

[0061] The extract may also be concentrated by evaporation of the solvent, or dried, for example, by freeze-drying or spray-drying. The extract will then be in the form of a powder.

[0062] In one specific embodiment of the present invention, particularly for use in dermatology, the obtained Moringa oleifera extract is sterilized.

[0063] In one particularly advantageous embodiment, the protein extract of unsprouted, de-oiled seeds of Moringa oleifera has a protein content, specifically natural protein, of 0.01 to 100% by weight, preferably at least 25% by weight, specifically at least 40% by weight, and more specifically at least 45% by weight, on a dry extract basis.

[0064] The protein in the protein extract according to the present invention has a molecular weight of 6,500 to 13,000 Da, and more preferably 7,100 to 11,000 Da, as measured by chromatography.

[0065] Specifically, the protein extract from ungerminated, de-oiled Moringa oleifera seeds does not contain alkaloids (e.g., spirotin), pterygospermin, isothiocyanates (e.g., 4-2-L-rhamnosiloxybenzyl isothiocyanate), or caenpferol.

[0066] The extract according to the present invention can be formulated in any form suitable for application to surfaces exposed to pollen, specifically the outer surface.

[0067] In one embodiment of the present invention, the extract is incorporated into a composition of hygiene and / or maintenance supplies, specifically a cleaning agent, comprising at least one further suitable excipient. The composition may also contain agents suited to other purposes, such as conventional disinfectants, cleaning agents, surfactants, and bactericides.

[0068] In another embodiment, the extract according to the present invention may be used in the form of a cosmetic or pharmaceutical ingredient, further comprising a suitable cosmetic or pharmaceutical medium, for the purpose of being incorporated into a cosmetic or pharmaceutical composition.

[0069] In this case, the extract according to the present invention is solubilized and / or diluted in, in another embodiment, preferably particularly polar, solvent, such as water, advantageously water further containing glycerin, such as a product commercially available under the names Purisoft® or Puricare®, specifically as described in Example 3a).

[0070] In another embodiment, the extract according to the present invention may be sprayed with a spray carrier, such as maltodextrin, to form a powder, specifically the form described in Example 3b).

[0071] Advantageously, when the components are in liquid form as described in Example 3a), the extract according to the present invention contains a dry substance content of 0.01 to 10% by weight, preferably 1 to 5% by weight, relative to the total weight of the components.

[0072] Advantageously, when the components are in solid form, particularly powder as described in Example 3b), the extract according to the present invention contains in the components a dry substance content of 10 to 60% by weight, preferably 30 to 50% by weight, relative to the total weight of the components.

[0073] In some cases, the extract according to the present invention, which is in the form of an ingredient in a cosmetic or skin product, may also be in the form of a cosmetic or pharmaceutical composition, which is intended for administration via a topical route, preferably to the skin, and further includes a suitable cosmetic or pharmaceutical medium.

[0074] The components of cosmetics or pharmaceuticals, specifically skin products, in liquid form and in particular in the form described in Example 3a) are used in the composition of cosmetics or pharmaceuticals, specifically skin products, preferably in a dry substance content of 0.01 to 10% by weight, preferably 0.1 to 5% by weight, specifically 1 to 3% by weight, relative to the total weight of the composition.

[0075] The components of cosmetics or pharmaceuticals, specifically skin products, in solid form and in particular in the form described in Example 3b) are used in compositions of cosmetics or pharmaceuticals, specifically skin products, preferably in a dry substance content of 0.001 to 5% by weight, and more preferably 0.01 to 1% by weight, relative to the total weight of the composition.

[0076] In one embodiment of the present invention, the extract is included in a cosmetic or pharmaceutical composition with a dry substance content of 0.0001% to 20% by weight, preferably 0.001% to 10% by weight, and more favorably 0.01% to 5% by weight, based on the total weight of the composition.

[0077] The compositions according to the present invention may contain any suitable solvent and / or any suitable medium and / or any suitable excipient, possibly in combination with other beneficial compounds. In particular, they may contain excipients acceptable for cosmetic or skin products, selected from surfactants, preservatives, buffers, leavening agents, chelating agents, biocides, denaturants, opacities, pH adjusters, reducing agents, stabilizers, emulsifiers, viscosity modifiers, gelling agents, film-forming polymers, solvents, additives, bactericides, deodorants, matting agents, conditioners, texturers, glossing agents, pigments, colorants, fragrances and chemical or inorganic UV inhibitors, trace elements, essential oils, sweeteners and taste modifiers. These combinations are also within the scope of the present invention. The CTFA Cosmetic Ingredient Handbook, Second Edition (1992) specifically lists various cosmetic and pharmaceutical ingredients commonly used in the cosmetic and pharmaceutical industries, suitable for oral administration and / or topical use.

[0078] Advantageously, one or more excipients are selected from the group comprising polyglycerols, esters, cellulose polymers and derivatives, lanolin derivatives, phospholipids, lactoferrin, lactoperoxidase, stabilizers mainly composed of sucrose, vitamin E and its derivatives, xanthan gum, natural and synthetic waxes, vegetable oils, triglycerides, unsaponifiables, phytosterols, silicones, protein hydrolysates, betaine, amine oxides, plant extracts, sucrose esters, titanium dioxide, glycine, and parabens, and more preferably steareth-2, steareth-21, glycol ether-15 stearyl, cetearyl alcohol, phenoxyethanol, methylparaben, ethylparaben, propylparaben, butylparaben, butylene glycol, caprylyl glycol, natural tocopherol, glycerin, sodium dihydroxycetyl phosphate, isopropyl hydroxycetyl ether, glycol stearate, triisononanoin, and ethylhexyl cocoate (octyl Selected from the group consisting of cocoate, polyacrylamide, isoparaffin, laureth-7, carbomer, propylene glycol, hexylene glycol, glycerol, bisabolol, dimethicone, sodium hydroxide, PEG-30 dipolyhydroxystearate, caprylic / capric triglyceride, cetearyl octanoate, dibutyl adipate, grape seed oil, jojoba oil, magnesium sulfate, EDTA, cyclomethicone, xanthan gum, citric acid, sodium lauryl sulfate, waxes and inorganic oils, isostearyl isostearate, propylene glycol dinonanoate, propylene glycol isostearate, PEG-8, beeswax, hydrogenated palm kernel oil fatty acid glycerides, lanolin oil, sesame oil, cetyl lactate, lanolin alcohol, castor oil, titanium dioxide, lactose, sucrose, low-density polyethylene, isotonic saline, and mixtures thereof.

[0079] In some cases, the cosmetic or pharmaceutical composition or extract according to the present invention, in the form of a cosmetic or pharmaceutical ingredient, can take any dosage form conventionally used for topical application (application), for example, in the form of a liquid or solid or a compressed liquid. In particular, it can be formulated in the form of anhydrous products, such as aqueous or oily solutions, particularly creams or aqueous or oily gels in bottles or tubes, particularly shower gels, shampoos, lotions (milks), emulsions, hydrogels, particularly oil-in-water or water-in-oil or multi-layer or silicone microemulsions or nanoemulsions, serums, particularly lotions, ampoules, liquid soaps, pastes, cleansing bars, pomades, mousses, aerosols, masks, patches, preferably in the form of liquids, pastes, or solids, such as sticks, particularly makeup powders. Specifically, the composition may take the form of a serum, lotion, cream, emulsion, pomade, paste, mousse, emulsion, hydrogel, shower gel, mask, stick, patch, or makeup powder, preferably a cream or lotion.

[0080] In the case of administration via a mucosal route, the cosmetic or pharmaceutical composition or extract according to the present invention may, in some cases, be in the form of a cosmetic or pharmaceutical ingredient, such as an eye wash, lotion, aerosol, gel, or mucosal-adhering composition.

[0081] The cosmetic or pharmaceutical composition may further include other components selected from the following for the treatment of the skin and / or mucous membranes and / or skin appendages, which complement or synergistically interact with the extract according to the present invention. - Combinations of sodium hyaluronate, pullulan, and sodium alginate, particularly formulations containing serine, trehalose, urea, and glycerin, which are marketed by the applicant under the name PatcH2O(trademark), - Cosmetic agents intended for the care of sensitive skin, such as the plant extract of Cestrum latifolium as described in International Patent Application No. 2009 / 112590, marketed by the applicant under the name Symbiocell (trademark); butter extracted from the fruit of the African mango tree (Irvingia gabonensis), marketed by the applicant under the name Irwinol (trademark); root extract of Eperua falcata, marketed under the name Eperuline (trademark); and peptide N-acetyl-L-tyrosyl-L-prolyl-L-phenylalanyl-L-phenylalaninamide (INCI: acetyl tetrapeptide-15), marketed by the applicant under the name Skinasensyl (trademark).

[0082] The cosmetic composition may further contain one or more other ingredients that act on the bacterial flora of the skin and / or mucus, and / or on the skin's barrier function, particularly those with moisturizing and / or soothing effects, such as oligosaccharides obtained by enzymatic synthesis and marketed by Solavia under the name BioEcolia®, or α-gluco-oligosaccharide complexes marketed by the same company under the name Ecoskin®, Alisma plantago-aquatica extract, Argania spinosa extract (Lipofructyl® Argan), ceramide mixture (Sphingoceryl® VEG), Boldo purified extract (Betapur®), inulin or a product mainly composed of fructooligosaccharides, Bifidobacterium extract, or Orthosiphon stamineus extract (MAT-XS®) for controlling oily skin. It may include, for its moisturizing properties, a natural extract of honey marketed by the applicant under the name Melhydran (trademark), a flaxseed extract marketed by the applicant under the name Oligolin (trademark), a yeast extract modified by biotechnology and marketed by the applicant under the name Relipidium (trademark), a kudzu root extract marketed by the applicant under the name Inhipase (trademark), a β-glucan derivative derived from baker's yeast marketed by Mibelle under the name CM-Glucan Forte (trademark), and / or a four o'clock flower extract marketed by Sederma under the name Pacifeel (trademark).

[0083] Advantageously, the present invention also relates to a surface treatment method for limiting and / or reducing the amount of pollen present on a surface, more specifically skin, mucous membranes, and / or skin appendages, preferably hair, by particularly preventing its adhesion and / or preventing pollen dispersal, wherein the surface is exposed to pollen grains, and the method comprises the following steps: - Specifically, in individuals, the range of skin and / or mucous membranes and / or skin appendages that may come into contact with pollen, the identification of surfaces that may come into contact with pollen, provided that the skin and / or mucous membranes and / or skin appendages are healthy skin and / or mucous membranes and / or skin appendages, and - Topical application (application) of a composition, specifically a cosmetic composition, comprising a protein extract of ungerminated, defatted seeds of Moringa oleifera according to the present invention, in an amount effective to limit and / or reduce the amount of pollen present on a surface, specifically the skin and / or mucous membranes and / or skin appendages, provided that the skin and / or mucous membranes and / or skin appendages are healthy skin and / or mucous membranes and / or skin appendages. Regarding methods including

[0084] The present invention also relates to the use of its use in the treatment and / or prevention and / or reduction of the occurrence thereof of pollen allergy and / or symptoms and / or signs related to pollen allergy, specifically "hay fever," rhinitis, sinusitis, conjunctivitis, tracheitis, watery eyes, sneezing, itching, headache, itchiness, anosmia, runny nose, nasal congestion, and / or asthma attacks, specifically for topical use, specifically protein extract of unsprouted defatted seeds of Moringa oleifera, advantageously protein extract of unsprouted defatted seeds of Moringa oleifera, specifically unsprouted seeds, more advantageously defatted cake of unsprouted seed kernels, more specifically protein extract of defatted cake.

[0085] The present invention is particularly intended for the care and / or pharmaceutical treatment, specifically dermatological treatment, of the skin, mucous membranes, and / or skin appendages, specifically hair, of humans with pollen allergies, i.e., humans whose type I hypersensitivity reaction is triggered by exposure to pollen grains.

[0086] In the context of this invention, "pollen allergy" refers to type I hypersensitivity caused by pollen antigens.

[0087] Other objects, features, and advantages of the present invention will be readily apparent to those skilled in the art by reading this description in light of the examples and figures shown herein, which are provided only as examples and not limiting the scope of the invention in any way.

[0088] The examples are an essential element of the present invention, and any feature that is considered novel to the prior art from the entirety of this specification, including the examples, is an essential element of the present invention in terms of its function and generality.

[0089] Therefore, each embodiment has a general range.

[0090] On the other hand, in the examples, unless otherwise specified, temperature is expressed in Celsius and pressure in atmospheric pressure. [Brief explanation of the drawing]

[0091] [Figure 1] This is a photograph of a hair mesh of a control sample treated with water without the extract according to the present invention, approximately 16 hours after being sprinkled with a predetermined amount of cedar pollen as observed under a microscope according to the procedure of Example 2. [Figure 2] This is a photograph of a hair mesh treated with water formulated according to Example 3a) containing 0.1% (by weight) of the extract from Example 1d, approximately 16 hours after sprinkling a predetermined amount of cedar pollen as observed under a microscope according to the procedure of Example 2. [Figure 3] This is a photograph of a hair mesh treated with water formulated according to Example 3a) containing 1% (by weight / by weight) of the extract from Example 1d, approximately 16 hours after sprinkling a predetermined amount of cedar pollen as seen under a microscope according to the procedure of Example 2. [Modes for carrying out the invention]

[0092] [Examples] [Example 1] Preparation of Moringa Extract According to the Present Invention Moringa extract is prepared by the method described in European Patent Application No. 1064008, specifically, for example, the method described below.

[0093] [Example 1a] (Preparation of extract 1a) Moringa oleifera kernels, obtained after removing the seed hulls and containing 33.4% (by weight / by weight) oil, were degreased by two consecutive reflux extractions in hexane, filtered, and the resulting powder was dried in a dryer at 40°C, exhibiting a residual oil content of 2.5%.

[0094] Add 200g of defatted powder to 2L of distilled water in the reactor. After stirring for 10 minutes, adjust the pH to 7.5 by adding 4N NaOH, and then extract at ambient temperature for 1 hour while maintaining the pH at 7.5. Remove insoluble matter by centrifugation of 5000g for 15 minutes.

[0095] The supernatant was collected and then filtered through 0.45 μm to obtain 1.77 L of a yellow filtrate containing 4.69% dry extract and showing a protein concentration of 21.54 g / L as measured by the biuret reaction (i.e., a protein purity of 45.92% based on the dry extract). The extract was spray-dried to obtain 65.72 g of atomized material with an estimated protein content of 54.7% (N x 6.25).

[0096] Considering the eluted peak between the excluded volume and the total column volume, the chromatographic profile of the gel permeation analysis results of this extract on a Superose 12HR column reveals a main fraction corresponding to a molecular weight located at 7,800–11,000 Da, representing 52% of the surface. The presence of shoulders in this peak indicates the presence of multiple compounds, and the molecular weight range is close to that found in the literature regarding monomers (6,500–7,000 Da) and dimers (13,000 Da) of moringa aggregated proteins.

[0097] [Example 1b] (Preparation of Extract 1b) 300 g of defatted powder was extracted according to Example 1a to obtain a crude aqueous extract. The pH of the filtrate (2.74 L) was adjusted to 11.8 by gradually adding 4N NaOH. Precipitation began at around pH 8.0 (the solution became clearly turbid), and after 30 minutes, the solution was centrifuged at 5000 g for 15 minutes. The viscous precipitate was collected (43.2 g in a wet state) and then washed twice with 500 mL of distilled water at pH 11.8.

[0098] Subsequently, the precipitate is dissolved in 270 mL of distilled water (i.e., 10% of the initial volume), and the pH of the solution is successively adjusted to 4.5 with 6N HCl to allow for the solubilization of the precipitate (dispersion is facilitated by using a device of the type known as Turax).

[0099] After stirring for 30 minutes, the mixture is centrifuged at 5000 g for 15 minutes to remove insoluble matter, and the supernatant is filtered through a Buchner filter equipped with a Whatman No. 41 filter. In this way, 260 mL of a clear yellow protein concentrate is obtained, which is dehydrated by freeze-drying.

[0100] This method yields 11.5g of freeze-dried material with a protein weight content of 90-95%.

[0101] Gel permeation analysis of this extract using a Superose 12HR column revealed a main fraction representing 70% of the surface and corresponding to a molecular weight of approximately 8,800 Da.

[0102] [Example 1c] (Preparation of Extract 1c) The oil from the kernels obtained by removing the hulls of Moringa oleifera seeds is extracted by pressing in a press known as KOMET, and the resulting cake is crushed to obtain a homogeneous powder.

[0103] Crude extracts are prepared from 1.24 kg of cake according to the operating procedures described in Examples 1a and 1b.

[0104] The protein is precipitated at pH 11.8 according to Example 1b, but an additional step of overnight decantation at +4°C is introduced to allow for better precipitation of the protein.

[0105] The precipitate is treated under the same conditions as in Example 1b (except that the pH of the precipitate reconstitution solution is 6 instead of 4.5).

[0106] The protein concentrate obtained in this manner (1.05 L of a 4.59% dry extract) was dehydrated by spray drying, and 34.6 g of atomized material was collected. This means that the yield after spray drying, based on the dry extract, was 71.5%.

[0107] The protein content based on nitrogen content (N x 6.25) exceeds 90% (approximately 95%).

[0108] [Example 1d] (Preparation of extract 1d) Crude extracts are prepared from 150 g of cake according to the procedures described in Examples 1a, 1b, and 1c.

[0109] After filtration through a 0.45 μm filter, 1.35 L of a clear yellow filtrate is obtained.

[0110] 100g of carboxymethylcellulose (CM52, WHATMAN) is allowed to stand in 500mL of distilled water with a pH of 7.5 for 30 minutes to reach equilibrium.

[0111] The mixture is filtered through a Buchner filter equipped with a WHATMAN No. 42, then the cellulose is collected and brought back to equilibrium with 500 mL of pH 7.5 water.

[0112] After removing the aqueous medium by filtration, the cellulose is brought into contact with the aqueous extract of Moringa oleifera kernel cake under stirring at ambient temperature for 1 hour.

[0113] Unadsorbed compounds (fractions shown by dotted lines in the chromatography profile in Figure 3) are removed by filtration with a Buchner filter, and the "packed" cellulose is then washed twice with 1 L of distilled water at pH 7.5, followed by filtration with a Buchner filter.

[0114] Subsequently, the cellulose is brought into contact with 120 mL of 60 g / L NaCl aqueous solution with a pH of 7.5 for 30 minutes.

[0115] The protein eluted with NaCl medium is collected by filtration using a Buchner filter (the chromatographic profile of the protein adsorbed with CM 52 and eluted with 60 g / L NaCl medium is shown as a solid line in Figure 3).

[0116] In this way, 110 mL of filtrate with a dry extract content of 9.63% and a protein concentration of 64.6 g / L (i.e., a protein purity of 67% based on the dry extract) is obtained.

[0117] Gel permeation analysis of this extract using a Superose 12HR column revealed a main fraction representing 70% of the surface and corresponding to a molecular weight of approximately 7,100 Da.

[0118] The solution may be desalted by dialysis or ultrafiltration, and dehydrated by freeze-drying, spray-drying, or any suitable method. According to Example 1d, the extract was spray-dried on a maltodextrin carrier and formulated according to Example 4b) for examination in subsequent examples.

[0119] [Example 2] Evaluation of the anti-pollen effect according to the present invention in the adhesion of pollen grains to hair Procedure: Preliminary studies were conducted and showed that pollen grains from Japanese cedar (Cryptomeria japonica) trees adhere strongly to hair mesh sprinkled with them in a sealed laboratory. Hair mesh (of average female length) was pre-treated for approximately 16 hours with the component obtained according to Example 1d and formulated according to Example 3a), using doses of 0.1% and 1% (by weight / by weight) of water relative to the total weight of the water / component mixture. The hair mesh was then brushed, a predetermined amount of cedar pollen was sprinkled onto the mesh, and the whole was stirred in a sealed laboratory for 5-10 minutes. Microscopic observation of the hair mesh allowed for visualization and quantification of the percentage of pollen grains attached to the hair by counting through image analysis. A control sample with only water and no component was performed in the same manner.

[0120] Results: Figures 1, 2, and 3 show the results obtained. This test shows that pre-application of the anti-pollen agent according to the present invention significantly and in a dose-dependent manner reduces the adhesion of pollen grains to the hair mesh. At 1% by weight in water, the components of Example 3a) reduced the amount of pollen grains adhering to the hair mesh by 70%.

[0121] Similar tests were repeated using fabric fibers instead of hair mesh, and in this case too, the product demonstrated a significant reduction in pollen adhesion.

[0122] [Example 3] Cosmetic or pharmaceutical ingredient according to the present invention, containing moringa extract according to the present invention [Example 3a)] Prepare a liquid component for a cosmetic or pharmaceutical having the following formulations expressed in weight percent. [Table 1]

[0123] [Example 3b)] Prepare a powdered solid component having the following composition. [Table 2]

[0124] [Example 4] Composition according to the present invention in the form of a body and / or face lotion [Table 3] The lotion is prepared by mixing six phases in a manner commonly known to those skilled in the art.

[0125] [Example 5] Composition according to the present invention in the form of a body and / or face lotion (milk) Cosmetics in the form of body and / or face lotions may have a weight composition, for example, as shown below, consisting of the following aqueous and fatty phases. [Table 4]

[0126] The method for preparing a facial and / or body lotion essentially consists of raising the fat phase to 80°C, raising the water in the aqueous phase to 80°C as well, dissolving a preservative (Elestab 4112) in it, then pouring the aqueous phase into the fat phase under turbine stirring, gradually cooling under stirring, then adding an aqueous mother solution of moringa protein extract at around 50°C, followed by the fragrance, and finally continuing stirring until completely cooled.

[0127] [Example 6] Composition according to the present invention in the form of a facial cream Cosmetics in the form of creams for the body and / or face may have a weight composition, for example, as shown below, consisting of the following aqueous and fatty phases. [Table 5]

[0128] The method for preparing the facial cream essentially involves raising the fat phase to 80°C, raising the aqueous phase to 80°C as well, dissolving Elestab 4112 in them, separately preparing a mother solution of Moringa oleifera protein extract, pouring the fat phase into the aqueous phase under turbine stirring, then introducing the mother solution of Moringa extract at approximately 50°C, and continuing stirring until it cools. The present invention includes, for example, the following embodiments: [Embodiment 1] Use of protein extracts of unsprouted, defatted seeds of Moringa oleifera, preferably protein extracts of unsprouted, defatted seeds, specifically of unsprouted seeds of Moringa oleifera, more preferably defatted cake of unsprouted seed kernels, more specifically protein extracts of defatted cake, as an anti-pollen agent. [Embodiment 2] The use according to Embodiment 1 for limiting and / or reducing the amount of pollen present on a surface, more specifically on the skin, mucous membranes, and / or skin appendages, specifically on the hair, in particular by preventing its adhesion and / or preventing its dispersal. [Embodiment 3] Use as a cosmetic according to either Embodiment 1 or 2, characterized in that a protein extract of ungerminated, defatted seeds of Moringa oleifera is applied to surfaces exposed to pollen, particularly surfaces of household items, clothing, skin, mucous membranes, and / or skin appendages, preferably hair. [Embodiment 4] Pollen from Betulaceae, specifically Betura, especially birch; Aulnus, especially alder; Corylus, especially European hazel; Cupressaceae, specifically cypress and cedar (Cryptomeria japonica), mountain cedar (Mulange cypress); Pinaceae, specifically Himalayan cedar; Asteraceae, specifically ragweed (Ambrosia) The use according to any one of Embodiments 1 to 3, characterized in that the pollen is from a plant selected from artemisiifolia, grasses (Poaceae), oak (Quercus), and / or plane trees (Platanus), more preferably from birch, hazel, cedar, Japanese cedar, rice, and / or ragweed. [Embodiment 5] The use according to any one of Embodiments 1 to 4, characterized in that the protein extract of ungerminated, defatted seeds of Moringa oleifera is applied topically (applied) to specific parts and / or areas of the body exposed to pollen, preferably the hair, selected from the face, hands, arms, décolleté, lip mucosa, nasal mucosa, and / or eye mucosa, body hair, eyelashes, eyebrows, and / or hair. [Embodiment 6] The use according to any one of Embodiments 1 to 5, characterized in that the protein extract of ungerminated, de-oiled seeds of Moringa oleifera is a water-soluble extract, specifically a water-soluble extract obtained by extraction with a polar solvent, preferably water. [Embodiment 7] The use according to any one of Embodiments 1 to 6, characterized in that the protein extract of ungerminated, de-oiled seeds of Moringa oleifera has a protein content of 0.01 to 100% by weight, preferably at least 25% by weight, on a dry extract basis, specifically natural protein. [Embodiment 8] The use according to any one of Embodiments 1 to 7, characterized in that the protein extract of ungerminated, defatted seeds of Moringa oleifera is an extract of seeds from which the hulls have been removed. [Embodiment 9] The use according to any one of Embodiments 1 to 8, characterized in that the protein extract of ungerminated, defatted seeds of Moringa oleifera is intended to be incorporated into a cosmetic composition and further comprises a suitable cosmetic medium, wherein the extract is in the form of a cosmetic ingredient. [Embodiment 10] The use according to Embodiment 9, characterized in that a protein extract of ungerminated, defatted seeds of Moringa oleifera is present in the cosmetic ingredient in a dry substance content of 0.01 to 10% by weight, preferably 1 to 5% by weight, relative to the total weight of the ingredient in liquid form, or in a content of 10 to 60% by weight, preferably 30 to 50% by weight, relative to the total weight of the ingredient in solid form. [Embodiment 11] The use according to any one of Embodiments 1 to 10, characterized in that a protein extract of ungerminated, oil-free seeds of Moringa oleifera or a cosmetic ingredient containing the same is in the form of a cosmetic composition further comprising a suitable cosmetic medium for administration via a topical route. [Embodiment 12] The use according to Embodiment 11, characterized in that a protein extract of ungerminated, defatted seeds of Moringa oleifera is present in the cosmetic composition in a dry matter content of 0.0001% to 20% by weight, preferably 0.01% to 5% by weight, relative to the total weight of the composition. [Embodiment 13] The use according to either Embodiment 11 or 12, characterized in that the composition is presented in the form of a serum, lotion, cream, emulsion, pomade, paste, mousse, emulsion, hydrogel, shower gel, aerosol, mask, stick, patch, or makeup powder, preferably a cream or lotion. [Embodiment 14] Protein extract of unsprouted, de-oiled seeds of Moringa oleifera for use in the treatment and / or prevention and / or reduction of the occurrence thereof of pollen allergy and / or symptoms and / or symptoms related to pollen allergy, specifically "hay fever", rhinitis, sinusitis, conjunctivitis, tracheitis, watery eyes, sneezing, itching, headache, itchiness, anosmia, runny nose, nasal congestion, and / or asthma attacks, specifically for topical use. [Embodiment 15] The protein extract of unsprouted, defatted seeds of Moringa oleifera for use according to Embodiment 14, characterized in that it is a protein extract of unsprouted, defatted seeds of Moringa oleifera as defined in any one of Embodiments 1 to 8. [Embodiment 16] The protein extract of unsprouted, defatted seeds of Moringa oleifera for use according to either Embodiment 14 or 15, characterized in that the protein extract of unsprouted, defatted seeds of Moringa oleifera is in the form of a pharmaceutical ingredient or pharmaceutical composition further comprising a suitable pharmaceutical medium.

Claims

1. Use of protein extract from ungerminated, oil-free seeds of Moringa oleifera as an anti-pollen agent (however, the said use does not involve medical treatment of humans).

2. The use according to claim 1 for limiting and / or reducing the amount of pollen present on a surface.

3. The use as a cosmetic according to claim 1 or 2, characterized in that a protein extract of ungerminated, defatted seeds of Moringa oleifera is applied to a surface exposed to pollen.

4. The use according to any one of claims 1 to 3, characterized in that the pollen is from a plant selected from the Betulaceae family; Cupressaceae family; Pinaceae family; Asteraceae family; Poaceae family; Quercus serrata and / or Plane tree.

5. The use according to any one of claims 1 to 4, characterized in that a protein extract of ungerminated, defatted seeds of Moringa oleifera is applied topically (applied) to specific parts and / or areas of the body exposed to pollen, selected from the face, hands, arms, décolleté, lip mucosa, nasal mucosa, and / or eye mucosa, body hair, eyelashes, eyebrows, and / or hair.

6. The use according to any one of claims 1 to 5, characterized in that the protein extract of ungerminated, defatted seeds of Moringa oleifera is a water-soluble extract.

7. The use according to any one of claims 1 to 6, characterized in that the protein extract of ungerminated, defatted seeds of Moringa oleifera has a protein content of 0.01 to 100% by weight on a dry extract basis.

8. The use according to any one of claims 1 to 7, characterized in that the protein extract of unsprouted, defatted seeds of Moringa oleifera is an extract of hulled seeds and / or a protein extract of unsprouted, defatted seeds and / or a protein extract of defatted, defatted pressed cake of unsprouted Moringa oleifera seeds.

9. The use according to any one of claims 1 to 8, characterized in that the protein extract of ungerminated, defatted seeds of Moringa oleifera is in the form of a cosmetic ingredient, intended for incorporation into a cosmetic composition, and further comprising a suitable cosmetic medium.

10. The use according to claim 9, characterized in that a protein extract of ungerminated, defatted seeds of Moringa oleifera is present in the cosmetic ingredient in a dry substance content of 0.01 to 10% by weight relative to the total weight of the ingredient in liquid form, or in a content of 10 to 60% by weight relative to the total weight of the ingredient in solid form.

11. The use according to any one of claims 1 to 10, characterized in that a protein extract of ungerminated, defatted seeds of Moringa oleifera or a cosmetic ingredient containing the same is in the form of a cosmetic composition intended for administration via a topical route and further comprising a suitable cosmetic medium.

12. The use according to claim 11, characterized in that a protein extract of ungerminated, defatted seeds of Moringa oleifera is present in the cosmetic composition in a dry matter content of 0.0001% to 20% by weight relative to the total weight of the composition.

13. The use according to any one of claims 11 or 12, characterized in that the composition is presented in the form of a serum, lotion, cream, emulsion, pomade, paste, mousse, emulsion, hydrogel, shower gel, aerosol, mask, stick, patch, or makeup powder.

14. A composition comprising a protein extract of ungerminated, defatted seeds of Moringa oleifera for use in the prevention and / or reduction of the onset of pollen allergies and / or symptoms related to pollen allergies.

15. The composition according to claim 14, characterized in that the protein extract of ungerminated, defatted seeds of Moringa oleifera is one of the definitions in any one of claims 1 to 8.

16. The composition according to any one of claims 14 or 15, further characterized in that it is in the form of a pharmaceutical ingredient or pharmaceutical composition, further comprising a suitable pharmaceutical medium.

Images