Methods to promote collagen production

Abstract

To provide a skin care set and a collagen production promoting method which efficiently can promote collagen production in the face, the neck, or both.SOLUTION: A skin care set includes a light irradiation device that emits red light with a peak in the range of 600-700 nm and skin cosmetics including at least one component (A) selected from coenzyme Q10, riboflavin, and riboflavin phosphate, and salts thereof. The skin care set is used in the face, the neck, or both. A collagen production promoting method uses the skin care set.SELECTED DRAWING: None

Description

[Technical Field]

[0001] The present invention relates to a skincare set and a method for promoting collagen production. [Background technology]

[0002] Various beauty treatments have been proposed to increase the amount of collagen and hyaluronic acid, which contribute to the improvement of nasolabial folds and fine wrinkles. For example, Patent Document 1 proposes promoting collagen production by applying skincare active ingredients to the skin and irradiating it with one or more types of light having discontinuous spectra with peaks in the wavelength ranges of 510-536 nm, 650-670 nm, and 768-792 nm.

[0003] Furthermore, Patent Document 2 proposes promoting hyaluronic acid production by applying a composition containing glucosamine hydrochloride to the skin and irradiating it with red light having a peak wavelength of approximately 600 nm to approximately 750 nm, near-infrared light having a peak wavelength of approximately 750 nm to approximately 1000 nm, or both. [Prior art documents] [Patent Documents]

[0004] [Patent Document 1] Special Publication No. 2018-507740 [Patent Document 2] Japanese Patent Publication No. 2020-2133 [Overview of the Initiative] [Problems that the invention aims to solve]

[0005] However, Patent Document 1 only provides numerous examples of active ingredients for skincare, without examining which ingredients can efficiently promote collagen production. Furthermore, obtaining a discontinuous spectrum with peaks in multiple wavelength ranges requires the use of multiple LEDs, which tends to complicate the equipment.

[0006] Furthermore, while Patent Document 2 examines the promotion of hyaluronic acid production, it does not examine the promotion of collagen production. The present invention has been made in view of the above circumstances, and aims to provide a skincare set and a method for promoting collagen production that can efficiently promote collagen production. [Means for solving the problem]

[0007] To achieve the above objectives, the present invention employs the following configuration. [1] A light irradiation device that irradiates red light with a peak in the range of 600-700 nm, The skin cosmetic comprises one or more components (A) selected from coenzyme Q10, riboflavin, and riboflavin phosphate ester or salt thereof. A skincare set for use on either the face and / or neck. [2] The skin care set according to [1], wherein the concentration of component (A) in the skin cosmetic is 0.001 to 10% by mass. [3] A method for promoting collagen production, comprising applying a skin cosmetic containing one or more components (A) selected from coenzyme Q10, riboflavin, and riboflavin phosphate ester or salt thereof to one or both of the face and / or neck, and irradiating with red light having a peak in the range of 600-700 nm. [4] The red light has an integrated energy of 0.04 to 100 J / cm². 2 The collagen production promotion method described in [3], wherein the irradiation is performed in such a manner. [Effects of the Invention]

[0008] According to the skincare set and collagen production promotion method of the present invention, collagen production can be efficiently promoted. [Brief explanation of the drawing]

[0009] [Figure 1] This is a perspective view showing an example of a light irradiation device used in the present invention. [Figure 2] It is a top view showing an example of a light irradiation plate in a light irradiation device. [Figure 3] It is a sectional view taken along line III-III of FIG. 2. [Figure 4] It is a plan view showing an example of a mask used in the present invention. [Figure 5] It is a perspective view showing an example of a state where a mask is worn on the face. [Figure 6] It is a perspective view showing an example of a method for promoting collagen production of the present invention. [Mode for Carrying Out the Invention]

[0010] <Skin Care Set> The skin care set of the present invention comprises a light irradiation device that irradiates red light having a peak in the range of 600 to 700 nm, and a skin cosmetic containing one or more components (A) selected from coenzyme Q10, riboflavin, and riboflavin phosphate ester or its salt. The skin care set of the present invention is applied to one or both of the face and the neck.

[0011] [Light Irradiation Device] The light irradiation device is a device that irradiates red light having a peak in the range of 600 to 700 nm. The peak wavelength is more preferably in the range of 620 to 670 nm.

[0012] An example of the light irradiation device will be described with reference to FIGS. 1 to 3. The light irradiation device 10 shown in FIG. 1 includes a light irradiation device main body 11, a pair of lead wires 12, and a pair of light irradiation plates 13 connected to the light irradiation device main body 11 by the pair of lead wires 12. In the light irradiation device 10, a power source such as a battery and a switch are provided in the light irradiation device main body 11.

[0013] The light irradiation device main body 11 shown in FIG. 1 is U-shaped and can be used by hanging it on the neck or the like. In addition to its primary role as a lead wire supplying power from the light irradiation device body 11 to the light irradiation plate 13, the lead wire 12 also functions as a gripping part for operating the light irradiation plate 13.

[0014] The area of ​​the light irradiation plate 13 is 1 to 65 cm². 2 Preferably, 4-40cm 2 It is preferable that it be so. The thickness of the light irradiation plate 13 is preferably 0.01 to 10 mm, and more preferably 0.5 to 5 mm.

[0015] Figures 2 and 3 show a specific example of the light irradiation plate 13. Figure 3 is a cross-sectional view taken along line III-III in Figure 2. The light irradiation plate 13 shown in Figures 2 and 3 has a structure in which a light source unit 15 consisting of LEDs is provided on one side of a substrate 14, and the entire structure is covered by a cover sheet 16. The light source unit 15 is connected to the light irradiation device body 11 by lead wires 12.

[0016] The substrate 14 is made of a material that can transmit light from the light source. For example, polyethylene terephthalate, polypropylene, acrylic, polystyrene, and silicone rubber can be used. The transmittance of the substrate 14 is preferably 10 to 99%, and more preferably 40 to 99%.

[0017] The light source unit 15 is a light source that emits red light with a peak in the range of 600 to 700 nm. More preferably, the peak wavelength of the light source unit 15 is in the range of 620 to 670 nm. It is preferable to use an organic light-emitting diode (organic LED) or an inorganic light-emitting diode (inorganic LED) as the light source unit 15.

[0018] The cover sheet 16 can be made of a resin such as polyethylene terephthalate. By providing the cover sheet 16, the light irradiation plate can be protected from sweat and water. Furthermore, protective tape 17 is wrapped around the connection point between the light source unit 15 and the lead wire 12. Polyurethane can be used as the material for the protective tape 17. The light irradiation plate 13 shown in Figures 2 and 3 is used with the lower side shown in Figure 3 facing inward (towards the face).

[0019] [mask] It is preferable to fix the light irradiation plate 13 to a desired location, such as the nasolabial folds, using a mask that adheres closely to the face, as this leaves the hands free. Masks that come into close contact with the face are preferably made of a stretchable sheet-like material. Specifically, the stretching force measured by the following test method is preferably 0.70 cm or more, more preferably 0.70 to 3.00 cm, even more preferably 0.90 to 2.22 cm, and particularly preferably 1.10 to 2.22 cm.

[0020] Because the elasticity is above a desirable lower limit, the mask adheres easily to the face, and sufficient pressure is applied to the light irradiation plate 13, making it easy to fix in the desired location. Furthermore, it is possible to apply not only light stimulation but also pressure stimulation to the skin in the desired area. Because the elasticity is below a desirable upper limit, the burden on the face when worn, especially the burden on the ears from the ear hooks, is reduced.

[0021] The stretching force can be measured as follows. First, a sheet-like substrate is cut into a 5cm x 2cm strip to create a test specimen. A weight (300g) is suspended from one end of the test specimen, and the specimen is stretched under load. The length of the specimen (L1) is measured after 15 minutes. After measuring L1, the weight is removed from the specimen, and the length of the specimen (L2) is measured after 5 seconds. The stretching force can be calculated from the values ​​of L1 and L2 using the following formula (1).

[0022] Stretching force (cm) = L1 (cm) - L2 (cm) ····(1)

[0023] Examples of sheet-like substrates include nonwoven fabrics, silicone rubber, and urethane sheets. Among these, nonwoven fabrics are preferred because they have moderate elasticity, water retention properties, and can impregnate a sufficient amount of skin cosmetic containing component (A).

[0024] The type of nonwoven fabric used as the sheet-like substrate is not particularly limited, but examples include nonwoven fabrics obtained by known manufacturing methods such as spunbond, meltblown, thermal bond, chemical bond, spunlace, and needle punch. Another example is a nonwoven fabric (hereinafter referred to as a three-dimensional crimped nonwoven fabric) obtained by subjecting a fiber web containing latent crimpable fibers to fiber entanglement treatment by the spunlace method, and then heating the fiber web after the entanglement treatment to induce three-dimensional crimping. Among these, three-dimensional crimped nonwoven fabrics are preferred as the sheet-like substrate. Three-dimensional crimped nonwoven fabrics make it easier to obtain good elasticity.

[0025] Examples of such three-dimensional crimped nonwoven fabrics include those described in Japanese Patent Publication No. 2008-285433, and commercially available products include JP-95 (manufactured by Daiwabo Polytech Co., Ltd.). Three-dimensional crimping refers to a spiral curvature or curl, and the resulting deformation of the crimped portion into a rounded shape, achieved by a stuffing box type crimper or the like.

[0026] Nonwoven fabric materials include polyester monofibers such as polyethylene terephthalate (PET) or composite fibers containing polyester. Examples of composite fibers include polyester and synthetic fibers such as polypropylene (PP), polyethylene (PE), and rayon, or natural fibers such as cotton and silk. Among these, PET monofibers or composite fibers (hereinafter collectively referred to as PET-based fibers) are preferred. PET-based fibers exhibit good elasticity and water retention, resulting in better cosmetic effects. Thus, by using a nonwoven fabric, more preferably a PET fiber nonwoven fabric, as the sheet-like base material, good elasticity can be easily obtained.

[0027] The basis weight of the sheet-like substrate is not particularly limited, but for example, 30 to 250 g / m². 2 Preferably, 70-150 g / m² 2 This is preferable. By ensuring the basis weight of the sheet-like substrate is above a preferred lower limit, sufficient strength is obtained, resulting in good handling during application. Furthermore, sufficient elasticity is achieved, making it easier to achieve a comfortable fit. Additionally, it facilitates the impregnation of a sufficient amount of the skin cosmetic described later. By keeping the basis weight of the sheet-like substrate below a desirable upper limit, it becomes easier to avoid excessive thickness and ensure a tight fit without twisting or wrinkling during installation. Furthermore, costs can be kept down.

[0028] The thickness of the sheet-like substrate can be determined considering the material of the sheet-like substrate and its usability. For example, the thickness of the sheet-like substrate is preferably 0.1 to 2.0 mm, and more preferably 0.2 to 1.0 mm. Within this range, it is easier to maintain the required strength and to install the substrate.

[0029] An example of a mask made of a sheet-like substrate is shown in Figure 4. The mask 20 in Figure 4 is made of a sheet-like substrate consisting of a first tensioning body 21 that covers the lower jaw and pulls the lower jaw toward the ear, and a second tensioning body 22 that is positioned above the first tensioning body 21, covers the upper jaw and nasolabial fold area, and pulls the upper jaw toward the ear.

[0030] The first tension member 21 and the second tension member 22 are connected by a left connecting portion 23a on the left side of the corner of the mouth and by a right connecting portion 23b on the right side of the corner of the mouth. Since the connecting portion of the first tension member 21 and the second tension member 22 is spaced apart on both sides, handling during application is easy. In addition, the mask 20 can be fitted tightly to the cheeks without twisting or wrinkling.

[0031] The transverse length of the first tensile body 21 (the length of the longest part in the transverse direction) can be determined considering the material of the sheet-like substrate, but is preferably 250 to 290 mm, and more preferably 260 to 280 mm. The longitudinal length of the first tensile body 21 (the length of the longest part in the longitudinal direction) can be determined considering the material of the sheet-like substrate, but is preferably 60 to 80 mm.

[0032] The lateral length of the second tension member 22 (the length of the longest part in the lateral direction) can be determined considering the material of the sheet-like substrate, but is preferably 250 to 300 mm, and more preferably 260 to 280 mm. The longitudinal length of the second tension member 22 (the length of the longest part in the longitudinal direction) can be determined considering the material of the sheet-like substrate, but is preferably 80 to 110 mm. The lateral length of the first tension member 21 and the lateral length of the second tension member 22 may be the same or different. However, from the viewpoint of reducing the burden on the ear when worn, it is preferable that they be the same or that the lateral length of the second tension member 22 is longer.

[0033] The first tensile body 21 and the second tensile body 22 may be formed by punching out a single sheet-like substrate, or they may be formed separately and then connected. From the viewpoint of production efficiency, the method of forming them by punching out a single sheet-like substrate is preferred. The first tensile body 21 and the second tensile body 22 may be made of the same material or different material.

[0034] The first tensioning body 21 is generally elliptical in shape. The left outer portion of the left connecting portion 23a, which is connected to the second tension body 22 at the upper end of the first tension body 21, is a convex curve that slopes downward to the left and outward from the left connecting portion 23a. Similarly, the right outer portion of the right connecting portion 23b, which connects to the second tension body 22, at the upper end of the first tension body 21 is a convex curve that slopes downward to the right and outward from the right connecting portion 23b. The lower end 21c of the first tension member 21 is a nearly flat convex curve.

[0035] The first tensioning member 21 is designed to cover the lower jaw when worn on the face. Furthermore, the first tensioning body 21 is provided with a pair of first ear hooks. Specifically, when worn on the face, the left side has a first ear hook hole 25 for the left ear, and the right side has a first ear hook hole 26 for the right ear.

[0036] The first ear hook hole 25 for the left ear is composed of a triangular hole 25a with one vertex facing left (right in the illustration), an extension notch 25b extending to the left (right in the illustration) from the aforementioned vertex of the triangular hole 25a, and a terminal notch 25c provided at the end of the extension notch 25b at a right angle to the extension notch 25b.

[0037] Similarly, the first ear hook hole 26 for the right ear is composed of a triangular hole 26a with one vertex facing to the right (left in the illustration), an extension notch 26b extending to the right (left in the illustration) from the aforementioned vertex of the triangular hole 26a, and a terminal notch 26c provided at the end of the extension notch 26b at a right angle to the extension notch 26b. The first tensioning body 21 is designed to pull the lower jaw toward the ears by attaching the first ear hook hole 25 for the left ear and the first ear hook hole 26 for the right ear to both ears.

[0038] The second tensioning body 22 is generally shaped like an arc, with the lower side being convex and the upper side concave. Of the lower end of the second tension body 22, the left outer portion of the left connecting portion 23a, which is connected to the first tension body 21, is a convex curve that rises outward to the left from the left connecting portion 23a. Similarly, the right outer portion of the right connecting portion 23b, which is connected to the second tension body 22, is a convex curve that rises upward from the right connecting portion 23b toward the right and toward the outside. The upper end 22c of the second tensioning body 22 is concave. The upper end 22c is positioned so that when worn on the face, the second tensioning body 22 covers both cheeks up to below the eyes. A protruding piece 24 is provided in the center of the upper end 22c to cover the upper part of the nose up to the nasal root.

[0039] The second tensioning member 22, when worn on the face, covers the upper jaw and nasolabial folds, and further covers both cheeks up to below the eyes. Furthermore, the second tensioning body 22 is provided with a pair of first ear hooks. Specifically, when worn on the face, the left side has a second ear hook hole 27 for the left ear, and the right side has a second ear hook hole 28 for the right ear.

[0040] The second ear hook hole 27 for the left ear is composed of a triangular hole 27a with one vertex pointing upward to the left (upper right in the illustration), an extension notch 27b extending upward to the left (upper right in the illustration) from the aforementioned vertex of the triangular hole 27a, and a terminal notch 27c provided at the end of the extension notch 27b at a right angle to the extension notch 27b.

[0041] Similarly, the second ear hook hole 28 for the right ear is composed of a triangular hole 28a with one vertex pointing upwards to the right (upper left in the illustration), an extension notch 28b extending upwards to the right (upper left in the illustration) from the aforementioned vertex of the triangular hole 28a, and a terminal notch 28c provided perpendicular to the extension notch 28b at the end of the extension notch 28b. The second tensioning body 22 is designed to pull the upper jaw toward the ears by attaching the second ear hook hole 27 for the left ear and the second ear hook hole 28 for the right ear to both ears.

[0042] The second tensioning body 22 also has a nasal insertion portion 32 formed therein. The nasal insertion portion 32 consists of a shallow V-shaped nasal tip notch 32a and left nasal wing notches 32b and right nasal wing notches 32c rising from both ends of the nasal tip notch 32a, and as a whole it forms a concave notch that follows the contour of the external nose.

[0043] When the device is worn, if the tip of the nose is inserted into the nasal insertion portion 32 and exposed, the upper part of the nasal insertion portion 32 of the second tensioning body 22 will lift up along the tip of the nose. The upper part of the nasal insertion portion 32 of the second tensioning body 22 is continuous with the convex piece 24, and together with the convex piece 24, it covers the entire nose up to the nasal root.

[0044] The portion enclosed by the first tensioning body 21 and the second tensioning body 22, and the left connecting portion 23a and the right connecting portion 23b, is the exposed opening portion 31. In this embodiment, the exposed opening portion 31 is spindle-shaped. When the mask 20 is worn on the face, the outer edges of the left connecting portion 23a and the right connecting portion 23b are located near the lower end of the nasolabial folds.

[0045] [Skin cosmetics] The skin cosmetic contains ingredient (A). Ingredient (A) is one or more selected from coenzyme Q10, riboflavin, and riboflavin phosphate ester or salt thereof. All of ingredient (A) are known to function as antioxidants and mitochondrial activators.

[0046] Coenzyme Q10 (coenzyme Q10) is mostly found in the body in its reduced form, and since it exhibits antioxidant properties, the reduced form is preferable. Riboflavin, also known as vitamin B2 or lactoflavin, is a physiologically active substance classified as a water-soluble vitamin. It consists of a heterocyclic isoaloxazine ring to which the sugar alcohol ribitol is bonded.

[0047] Riboflavin phosphate esters are formed by esterifying the hydroxyl group of riboflavin with phosphoric acid. While there are no limitations on the position of the hydroxyl group to be esterified, it is preferable to use the hydroxyl group furthest from the heterocyclic isoalloxazine ring, as this is readily available. Examples of riboflavin phosphate salts include sodium salt, magnesium salt, potassium salt, and calcium salt, but sodium salt is preferred because it is readily available.

[0048] The concentration of component (A) in the skin cosmetic (if it is two or more components selected from coenzyme Q10, riboflavin, and riboflavin phosphate ester or salt thereof, the total concentration of each component) is preferably 0.001 to 10% by mass, more preferably 0.01 to 1% by mass, and even more preferably 0.02 to 0.4% by mass. When the concentration of ingredient (A) in the skin cosmetic is within a favorable range, a high collagen production promoting effect can be obtained.

[0049] For example, if the concentration of coenzyme Q10 in a skin cosmetic with water as the solvent is 1 μM, then it is approximately 0.0001% (w / w) = (1 × 10⁻¹⁰ -6 It can be converted as follows: mol / L × 863.37 g / mol (molecular weight of coenzyme Q10) ÷ 1000 g / L (weight of 1 L of water) × 100.

[0050] Considering that hair follicles are the pathway by which coenzyme Q10, a high molecular weight of 500 or more, reaches the dermis from the skin surface, and taking into account that hair follicles occupy 0.1% of the skin surface area, the concentration of coenzyme Q10 in a cosmetic product required to act on 1 μM of coenzyme Q10 in dermal fibroblasts is estimated to be 0.1% ("0.000001 ÷ 0.001") (Drug Delivery System 20-4, 2005, 452-459).

[0051] Furthermore, if the concentration of riboflavin phosphate sodium in a skin cosmetic with water as the solvent is 1 μM, then it is approximately 0.00005% (w / w) = (1 × 10⁻¹⁰ -6 It can be converted as follows: mol / L × 478.33 g / mol (molecular weight of riboflavin phosphate sodium) ÷ 1000 g / L (weight of 1 L of water) × 100.

[0052] Considering that riboflavin phosphate sodium, a high molecular weight with a molecular weight of nearly 500, reaches the dermis through hair follicles, and that hair follicles occupy 0.1% of the skin's surface area, the concentration of riboflavin phosphate sodium in a cosmetic product required to act on 1 μM coenzyme Q10 in dermal fibroblasts is estimated to be 0.05% (0.0000005 ÷ 0.001).

[0053] The skin cosmetic composition may contain other ingredients as long as they do not impair the effects of the present invention. For example, from the viewpoint of improving the adhesion of the mask to the skin and enhancing the moisturizing effect, it is preferable to include polyhydric alcohols such as glycerin, dipropylene glycol, 1-3-butylene glycol, polyethylene glycol, sorbitol, and propylene glycol.

[0054] The amount of polyhydric alcohol in a skin cosmetic can be determined by considering the desired effects of the skin cosmetic, but 10 to 50% by mass is preferred, and 15 to 30% by mass is more preferred. If the polyhydric alcohol content is above the above-mentioned preferred lower limit, sufficient mask adhesion and moisturizing effect can be obtained, giving the user the feeling that their nasolabial folds and fine wrinkles have become less noticeable. Furthermore, if the polyhydric alcohol content is below the above-mentioned preferred upper limit, the sticky feeling on the skin after use can be suppressed.

[0055] When the sheet-like substrate constituting the mask is impregnated with skin cosmetics, it can promote collagen production and, through its moisturizing effect on the stratum corneum, provide moisture and firmness to the skin. In addition, impregnation with skin cosmetics improves the adhesion between the sheet-like substrate and the skin, allowing the light irradiation plate to be more securely fixed to the skin. Furthermore, from the perspective of ensuring good handling during wear and preventing a decrease in wearing comfort, it is preferable not to impregnate the area around the ear hook hole, which comes into contact with the ear during wear, with skin cosmetics.

[0056] When impregnating a sheet-like substrate with a skin cosmetic, the skin cosmetic may be pre-impregnated into the sheet-like substrate by the manufacturer, or the user may impregnate the sheet-like substrate with the skin cosmetic at the time of use. Methods for impregnating a sheet-like substrate with skin cosmetics can be known, such as applying the product to the area to be impregnated with a brush or spraying it.

[0057] There are no particular limitations on the amount of skin cosmetic to be impregnated into the sheet-like substrate, but the impregnation ratio, expressed as the mass ratio (D / C) of the mass (C) of the sheet-like substrate in the part to be impregnated with the skin cosmetic (hereinafter referred to as the impregnation target part) to the mass (D) of the skin cosmetic to be impregnated, is preferably 5 to 13, and more preferably 7 to 20.

[0058] When the impregnation ratio is above a preferred lower limit, it becomes easier for the light to adhere to the skin and the light irradiation plate. Furthermore, by keeping the impregnation ratio below a desirable upper limit, it is easier to avoid problems such as the skin cosmetic dripping. The specific mass ratio can be determined by considering the material of the sheet-like substrate and the composition of the skin cosmetic.

[0059] [Methods to promote collagen production] Using Figures 5 and 6, we will explain a method for promoting collagen production using the light irradiation device 10 in Figure 1 and the mask 20 in Figure 4. Figure 5 is a perspective view showing an example of the mask 20 being worn on the face, and Figure 6 shows a method for promoting collagen production in which the light irradiation plate 13 of the light irradiation device 10 is fixed to the nasolabial fold area.

[0060] Note that Figures 5 and 6 are perspective views taken from the left side of the face, but the same applies when the device is attached to the right side of the face. Also, in Figure 6, only the light irradiation plate 13 and a portion of the lead wires 12 of the light irradiation device 10 are shown, and the light irradiation device body 11 and the lead wires 12 near the light irradiation device body 11 are not shown.

[0061] The skin cosmetic may be applied to the skin, such as the face, using cotton or hands before wearing the mask 20, or it may be impregnated into all or part of the sheet-like base material that constitutes the mask 20. If the skin cosmetic is impregnated into the sheet-like base material that constitutes the mask 20, it is preferable to apply the light irradiation plate 13 of the light irradiation device 10 to the skin after wearing the mask 20 on the face and allowing the skin cosmetic impregnated into the sheet-like base material to be thoroughly absorbed into the skin.

[0062] As shown in Figure 5, when the mask 20 is worn on the face, the first tension member 21 covers from the lower jaw 82 to the ear 81. The second tension member 22 covers from the upper jaw 83 to the ear 81, and its upper end reaches below the eye 87. As a result, the entire cheek 84 is covered by the first tension member 21 and the second tension member 22. The mouth 86 is exposed at the mouth exposure portion 31. Furthermore, the upper end of the convex piece 24 reaches the nasal root 88c, and the entire nose 88 from the nasal tip 88b to the nasal root 88c is covered by the second tensioning body 22 and the convex piece 24, except for the area around the columella 88a which is exposed from the nasal insertion part 32.

[0063] Furthermore, when the mask 20 is worn on the face, an overlapping portion 35 is created between a part of the first tensioning body 21 and a part of the second tensioning body 22. In the overlapping portion 35, the first tensioning body 21 is on the inside (skin side) of the second tensioning body 22. As shown in Figure 5, the overlapping portion 35 is located outside both corners of the mouth 86a, and is formed in a roughly triangular shape from near the lower end of both nasolabial fold portions 85 to both ears 81.

[0064] Mask 20 can be attached to the face in the following way, for example: First, the left-right center of the first tensioning body 21 is brought into contact with the lower jaw 82 so that the mouth 86 is exposed from the mouth exposure portion 31, and the first ear hook hole 25 for the left ear and the first ear hook hole 26 for the right ear (see Figure 4) are hooked onto both ears 81, respectively.

[0065] Next, the left-right center of the second tensioning body 22 is brought into contact with the upper jaw 83 and nose 88 so that the area around the columella 88a of the nose 88 is exposed from the nasal insertion portion 32 and the mouth 86 is exposed from the mouth exposure portion 31, and the second ear hook hole 27 for the left ear and the second ear hook hole 28 for the right ear (see Figure 4) are hooked onto both ears 81, respectively.

[0066] In this way, by wearing the mask 20, the first tensioning body 21 pulls the lower jaw 82 toward the ear 81. The second tensioning body 22 also pulls the upper jaw 83 toward the ear 81. As a result, the mask 20 adheres tightly to the face under pressure. Note that the mask 20 may be worn on the face by overlapping the second ear-hanging hole 27 for the left ear on the first ear-hanging hole 25 for the left ear in advance, and overlapping the second ear-hanging hole 28 for the right ear on the first ear-hanging hole 26 for the right ear, and then hanging these ear-hanging holes on both ears 81 at once.

[0067] Next, the light irradiation plate 13 is inserted between the first tension body 21 and the second tension body 22 from each of the lower ends of the overlapping portions 35 on both sides. After insertion, since most of the lead wire 12 is arranged outside the mask 20, the portion outside the overlapping portion 35 of the lead wire 12 is used as a gripping portion, and by moving this, the light irradiation plate 13 can be operated and appropriately moved. Then, when the position of the light irradiation plate 13 is adjusted to the wrinkle line portion 85, the operation of the light irradiation plate 13 by the lead wire 12 is terminated.

[0068] When the position of the light irradiation plate 13 is adjusted to the wrinkle line portion 85 and the operation of the light irradiation plate 13 by the lead wire 12 is terminated, the light irradiation plate 13 is pressed by the expansion and contraction force of the sheet-like base material constituting the mask 20 and fixed to the wrinkle line portion 85. Then, by supplying power from the light irradiation device main body 11 (not shown) to the light source portion 15 of the light irradiation plate 13, red light having a peak in the range of 600 to 700 nm can be irradiated onto the wrinkle line portion 85.

[0069] Irradiation of red light on the skin surface is preferably performed so that the energy integrated amount is 0.04 to 100 J / cm 2 preferably so that it becomes 0.2 to 20 J / cm 2 more preferably so that it becomes 4 to 10 J / cm 2 When the energy integrated amount of the red light irradiated on the skin surface is within the preferable range, a high collagen production promoting effect can be obtained.

[0070] ​It is estimated that approximately 20-50% of red light reaching the skin surface, particularly light with a wavelength of 650 nm, penetrates the dermis layer, which is about 0.2-3 mm deep (Journal of the Imaging Society of Japan, Vol. 56, No. 5, pp. 466 (2017)) (estimated by extrapolating the graph from the data in Fig. 5). Therefore, for example, 2 J / cm³ of dermal fibroblasts 2 To apply this red light, approximately 4-10 J / cm² is needed. 2 (2J / cm 2 ÷0.5, 2 J / cm 2 It is necessary to irradiate the skin surface with light at a concentration of ÷0.2).

[0071] The integrated energy of light is calculated as the product of the irradiance and the exposure time. The irradiance of light reaching the skin surface is 10-600 W / m². 2 Preferably, the wattage is 20-100 W / m². 2 It is preferable that it be so. If the irradiance of light reaching the skin surface is above the lower limit of the preferred range, the integrated energy of the light reaching the skin surface can be quickly increased to the desired integrated amount. If the irradiance of light reaching the skin surface is below the upper limit of the preferred range, excessive irritation to the skin can be avoided. The light irradiation time should be set appropriately according to the irradiance so that the integrated energy falls within the desired range.

[0072] The light irradiation plate 13, under pressure from the mask 20, applies compressive stimulation to the skin of the nasolabial fold area 85. At the same time, the second tensioning body 22 covering the light irradiation plate 13 is pulled in the direction of the ear 81, so the light irradiation plate 13 acts to compress the skin while simultaneously lifting it. Therefore, a skin-lifting effect on the nasolabial fold area 85 can also be expected.

[0073] The mask 20 covers the cheeks 84 up to below the eyes 87, and the convex piece 24 covers the nose 88 up to the nasal root 88c, making it easy to stably maintain the position of the light irradiation plate 13 in the nasolabial fold area 85. Therefore, it is possible to reliably promote collagen production in the nasolabial fold area 85. Furthermore, light leakage from the light source 15 of the light irradiation plate 13 is less likely to cause glare. Also, if the mask 20 is impregnated with skin cosmetics, the skin cosmetics can be penetrated not only into the nasolabial fold area where light is irradiated from the light irradiation plate 13, but also into the entire cheek 84 and nose.

[0074] The specific shape of the mask used to fix the light irradiation device 10 to the skin is not limited to the mask 20 described above. Furthermore, the collagen production promotion method of the present invention is not limited to a method using a light irradiation device with a light irradiation plate 13 and a mask to fix the light irradiation plate 13. For example, the light irradiation plate 13 may be directly held by hand and applied to the face or neck. Alternatively, a device may be used that has a light source and a skin cosmetic outlet at the tip of a rod-shaped body. [Examples]

[0075] [culture] Human adult-derived dermal fibroblasts were maintained at 37°C in petri dishes containing Dulbecco's Modified Eagle Medium (DMEM, Nacalai Tesque), supplemented with 5 vol% Fetal Bovine Serum (FBS, Thermo Fisher Scientific), and antibiotics 100 μg / mL penicillin and 100 μg / mL streptomycin. Subsequently, 6 × 10¹⁶ units were added to a 24-well plate containing 5 volume% FBS in DMEM. 4 The cells were individually seeded and cultured for 24 hours at a temperature of 37°C.

[0076] [Addition of functional ingredients] After culturing, the culture medium was replaced with DMEM without phenol red, supplemented with 2% by volume of FBS and the functional components shown in Table 1 or Table 2. When adding the functional components, water-soluble functional components were dissolved directly in the culture medium. Oil-soluble functional components were dissolved using dimethyl sulfoxide as a solvent before being added to the culture medium. Note that the concentrations of functional components shown in Table 1 or Table 2 are molar concentrations per unit volume of culture medium.

[0077] The functional ingredients listed in Tables 1 and 2 are as follows: Coenzyme Q10: CAS RN: 303-98-0 (manufactured by Tokyo Chemical Industry Co., Ltd.). Riboflavin sodium phosphate: CAS RN: 130-40-5 (manufactured by Fujifilm Wako Pure Chemical Industries, Ltd.).

[0078] Niacinamide: Nicotinamide, CAS RN: 98-92-0 (manufactured by Junsei Chemical Co., Ltd.). 3-o-ethyl ascorbic acid: CAS RN: 86404-04-8 (manufactured by Tokyo Chemical Industry Co., Ltd.). L-ascorbic acid: CAS RN: 50-81-7 (manufactured by Fujifilm Wako Pure Chemical Corporation). Glutathione: CAS RN: 70-18-8 (manufactured by Nacalai Tesque Co., Ltd.).

[0079] [Light irradiation] After adding the functional ingredients, the samples were irradiated with light at a peak wavelength of 634 nm at room temperature (25°C, the same applies hereafter) under the conditions shown in Table 1 or Table 2. A surface-emitting LED lighting device (TMN150x180-22RD-4) manufactured by ITEC Systems Co., Ltd. was used as the light irradiation device. Furthermore, after adding the functional ingredient, the samples were left at room temperature for the same amount of time as the irradiation time in each example, without light irradiation, and these were used as the control for each example.

[0080] [Collagen Quantitative Analysis] After the photoirradiation treatment was completed (or after the same time as the irradiation period for the control), the samples were left at 37°C for 48 hours. The supernatant was sampled, and the type I procollagen concentration was measured by ELISA (Takara Bio Inc., product code MK101). Furthermore, cell proliferation was evaluated using the Premix WST-1 Cell Proliferation Assay System (Takara Bio, product code MK400) after culturing to determine the number of cells after culturing. The amount of collagen produced per cell was then calculated by dividing the measured type I procollagen concentration by this number of cells.

[0081] [Evaluation of the synergistic effect of functional ingredients and light irradiation] The synergistic effect of the functional component and light irradiation in each example was evaluated according to the evaluation criteria below, based on the acceleration ratio obtained by equation (2) below.

[0082]

number

[0083] (Evaluation Criteria) ◎: Acceleration multiplier is 2.05 times or higher. ○: Acceleration ratio is 1.20 times or more, and less than 2.05 times. △: Acceleration multiplier is 1.00x or higher, and less than 1.20x. ×: The acceleration ratio is less than 1.00x.

[0084] [Table 1]

[0085] [Table 2]

[0086] As shown in Table 1, the examples using coenzyme Q10 or riboflavin sodium phosphate both showed high promotion ratios, indicating that a high collagen production-promoting effect can be obtained through the synergistic effect of component (A) and red light. On the other hand, as shown in Table 2, in comparative examples where other functional ingredients were used instead of ingredient (A), no synergistic effect between each ingredient and red light was observed at all. [Explanation of Symbols]

[0087] 10 Light irradiation device 11. Light irradiation device main unit 12 Lead wires 13 Light irradiation plate 14 circuit boards 15 Light source section 16 cover sheets 17 Protective tape 20 masks 21 First tensile body 22 The second tensile body 23a Left connection part 23b Right connection part 24 Convex piece 25 First ear hook hole for the left ear 26 First ear hook hole for the right ear 27. Second ear hook hole for the left ear 28. Second ear hook hole for the right ear. 31 Exposed mouth part 32 Nasal insertion part 35 Overlapping parts

Claims

1. A method for promoting collagen production, comprising applying a skin cosmetic containing one or more components (A) selected from coenzyme Q10, riboflavin, and riboflavin phosphate ester or salt thereof to one or both of the face and / or neck, and irradiating with red light having a peak in the range of 600 to 700 nm.

2. The aforementioned red light has an integrated energy of 0.04 to 100 J / cm² on the skin surface. ２ A method for promoting collagen production according to claim 1, wherein the irradiation is performed in such a manner.

3. The collagen production promoting method according to claim 1 or 2, wherein the concentration of component (A) in the skin cosmetic is 0.001 to 10% by mass.

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