Skin-improving cosmetic composition containing low-molecular-weight collagen, collagen water, or extract

Abstract

To provide a skin-improving cosmetic composition containing low-molecular-weight collagen, collagen water, or collagen extract. [Solution] A skin-improving cosmetic composition containing low molecular weight collagen, collagen water, or extract contains at least one collagen selected from low molecular weight collagen powder, collagen water, or collagen extract, wherein the collagen powder used has a molecular weight of 200 to 300 daltons (Da). With the above configuration, a skin-improving cosmetic composition containing low molecular weight collagen, collagen water, or extract according to various embodiments of the technical concept of the present invention can improve skin health by using low molecular weight collagen, collagen water, or collagen extract as a cosmetic composition, thereby exhibiting excellent skin-soothing, moisturizing, and skin barrier-improving effects.

Description

[Technical Field]

[0001] The present invention relates to a skin-improving cosmetic composition containing low molecular weight collagen, collagen water, or an extract, and more particularly to a skin-improving cosmetic composition containing low molecular weight collagen, collagen water, or an extract that, when used as a cosmetic composition, exhibits excellent skin-soothing, moisturizing, and skin barrier-improving effects, thereby improving skin health. [Background technology]

[0002] Modern society generates large amounts of reactive oxygen species due to exhaust fumes, harmful chemicals, ozone layer depletion, ultraviolet radiation, and water pollution. These reactive oxygen species penetrate the human body and cause various side effects, and are particularly known to be the main cause of pigment changes and skin aging.

[0003] Furthermore, in recent years, outdoor leisure activities such as golf have increased as a way to relieve excessive social and work-related stress. However, during such outdoor leisure activities, the skin is more easily exposed to the aforementioned sources of pollution, making skin damage and aging unavoidable. This has a negative impact on the tendency to enjoy outdoor leisure activities more actively and comfortably.

[0004] Generally, the skin plays a primary role in protecting our bodies from various external factors that disrupt homeostasis through basic metabolic processes. In other words, being the outermost layer of the body, the skin performs various functions such as protection through physical, chemical, and biological barrier functions, thermoregulation, sensory functions, secretory and excretory functions, absorption functions, storage functions, and regeneration functions. However, it is the most vulnerable to damage when subjected to sustained external stress.

[0005] The main causes of such external stress include ultraviolet radiation exposure, exhaust fumes, yellow dust, environmental pollutants, heavy metals, temperature changes, and microorganisms. These primarily generate reactive oxygen species (ROS) through oxidative stress, which disrupt the antioxidant defense system and cause cell damage and death.

[0006] Traditionally, cosmetics that provide elasticity to the skin, suppress the formation of wrinkles, and prevent skin aging have included cosmetics containing moisturizing ingredients such as collagen, elastin, natural moisturizing factors (NMOs), and glycerin, as well as cosmetics containing cell regeneration ingredients such as vitamins A, C, E and their derivatives, licorice extracted from plants, beta-carotene, and amino acids involved in signal transduction in fibroblasts.

[0007] Conventional cosmetics like these have several problems: when exposed to ultraviolet light and air for extended periods, the raw materials used in them can precipitate through recrystallization or discolor; in addition, they are difficult to maintain their potency, resulting in insufficient efficacy and potentially causing side effects on the skin.

[0008] Furthermore, in recent years, risk factors for the skin have been increasing. Changes in dietary habits have slowed down the rate of keratinization and shedding of the stratum corneum, and the decline in the function of keratin-forming cells has reduced the amount of moisturizing factors and lipids in the stratum corneum. As a result, the number of people with skin in which the stratum corneum cannot perform its normal skin barrier function is increasing.

[0009] Such impairment of the skin barrier function can trigger various skin diseases such as xerosis, atopic dermatitis, contact dermatitis, and psoriasis. While these conditions can be alleviated with conventional moisturizers that have normal water-retaining properties, a complete cure is difficult to achieve.

[0010] Therefore, the inventors have completed the present invention by confirming that a cosmetic composition containing low-molecular-weight collagen, collagen water, or collagen extract has excellent skin-soothing, moisturizing, and skin barrier-improving effects, thereby improving skin health. [Prior art documents] [Patent Documents]

[0011] [Patent Document 1] Republic of Korea Registered Patent No. 10-2139471 (Registered July 24, 2020) [Patent Document 2] Republic of Korea Published Patent No. 10-2023-0120546 (Published August 17, 2023) [Patent Document 3] Republic of Korea Published Patent No. 10-2023-0119873 (Published August 16, 2023) [Patent Document 4] Republic of Korea Patent Publication No. 10-2004-0106893 [Patent Document 5] Republic of Korea Patent Publication No. 10-2005-0079478 [Patent Document 6] Registered Patent No. 10-0810006 of the Republic of Korea [Patent Document 7] Registered Patent No. 10-0521845 of the Republic of Korea [Patent Document 8] Registered Patent No. 10-0498277 of the Republic of Korea [Patent Document 9] Registered Patent No. 10-1093730 of the Republic of Korea [Patent Document 10] Registered Patent No. 10-1156636 of the Republic of Korea [Patent Document 11] Registered Patent No. 10-1086669 of the Republic of Korea [Patent Document 12] Registered Patent No. 10-1086225 of the Republic of Korea [Patent Document 13] Registered Patent No. 10-0779179 of the Republic of Korea [Patent Document 14] Republic of Korea Patent Registration No. 10-1225486

Non-Patent Literature

[0012]

Non-Patent Literature 1

Non-Patent Literature 2

Non-Patent Literature 3

Summary of the Invention

Problems to be Solved by the Invention

[0013] The problem to be solved by the present invention is to provide a skin-improving cosmetic composition containing low-molecular-weight collagen, collagen water or a collagen extract, which, when used as a cosmetic composition, has excellent effects of calming the skin, moisturizing the skin and improving the skin barrier, and can improve the health of the skin.

[0014] Another problem to be solved by the present invention is to provide a skin-improving cosmetic composition containing low-molecular-weight collagen, collagen water or an extract, which can not only remove makeup, but also cleanly remove keratin on the skin and waste substances in pores, minimize skin troubles and skin irritation, and make the dry and rough skin smooth and moist.

[0015] The various problems to be solved by the present invention are not limited to the problems mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

Means for Solving the Problems

[0016] In one embodiment of the technical concept of the present invention, a skin-improving cosmetic composition containing low molecular weight collagen, collagen water, or an extract is disclosed.

[0017] The skin-improving cosmetic composition containing the low molecular weight collagen, collagen water, or extract comprises at least one collagen selected from low molecular weight collagen powder, collagen water, or collagen extract, wherein the collagen powder used is collagen powder with a molecular weight of 200 to 300 daltons (Da).

[0018] The low molecular weight collagen powder may be produced by preparing at least one collagen selected from the group consisting of fish, collagen extracted from fish scales, chicken foot collagen, cow foot collagen, and pig foot collagen, sterilizing the collagen, mixing 80-120 parts by weight of the sterilized collagen, 20-40 parts by weight of proteolytic enzyme, and 150-250 parts by weight of purified water to produce a collagen enzyme mixture, heating the collagen enzyme mixture in a water bath at a temperature of 57-635°C for 1-5 hours to produce a collagen enzyme hydrolysate, immersing the collagen enzyme hydrolysate in sodium hypochlorite for 10-60 minutes to decolorize and deodorize, concentrating the decolorized and deodorized collagen enzyme hydrolysate to produce a collagen concentrate, and spray-drying the collagen concentrate at a temperature of 160-180°C.

[0019] The collagen water may be produced by preparing at least one collagen selected from the group consisting of fish, collagen extracted from fish scales, chicken foot collagen, cow foot collagen, and pig foot collagen, sterilizing the collagen, adding distilled water in an amount of 10 to 30 times (v / w) to the sterilized collagen, heating the mixture at 60 to 80°C for 10 to 50 minutes, then distilling and extracting while heating at 100°C, recovering the distillate at 30 to 40% of the total weight of the mixture to produce a collagen distillation extract, and filtering the collagen distillation extract through a filter.

[0020] The collagen extract may be prepared by preparing at least one collagen selected from the group consisting of fish, collagen extracted from fish scales, chicken foot collagen, cow foot collagen, and pig foot collagen, sterilizing the collagen, mixing it with purified water in a weight ratio of 80 to 120 parts by weight per 100 parts by weight of the total content of the sterilized collagen, heating it at a temperature of 90 to 100°C for 1 to 3 hours to perform hot water extraction to produce a collagen hot water extract, and then filtering the collagen hot water extract through a filter.

[0021] In addition to the aforementioned low molecular weight collagen powder, collagen water, and collagen extract, the following are added: caffeic acid, soy-derived lysolecithin, herbal fermentation extract, coenzyme Q10, tocopherol acetate, butylene glycol, stearyltrimethylammonium chloride, L-aspartic acid, sorbitol, stearyl alcohol, cyclopentasiloxane, vitamin C, madecassoside, olive liquid, sodium laureth sulfate, cocamide MIPA, brewer's yeast extract, acai berry extract, emollient oil, inchin extract, sansai extract, grapefruit seed extract, sodium alginate, pine bark extract, pine needle sprout extract, panthenol, and salicylic acid. It may also contain Acid, Polyquaternium-7, Niacinamide, Jojoba Seed Oil, Behenyl Alcohol, Myristyl Alcohol, Cyclodextrin, Fragrance, and the remainder being deionized water.

[0022] The aforementioned low molecular weight collagen powder 0.1-0.3% by weight, collagen water 0.5-1.5% by weight, collagen extract 0.1-1% by weight, caffeic acid 0.1-1% by weight, soy-derived lysolecithin 0.1-0.5% by weight, herbal fermented extract 0.5-1.5% by weight, coenzyme Q10 Q10) 0.08-0.12% by weight, Tocopherol acetate 0.1-0.3% by weight, Butylene glycol 0.5-1.5% by weight, Stearyltrimethylammonium chloride 0.1-0.3% by weight, L-Aspartic acid 0.08-0.12% by weight, Sorbitol 0.05-0.15% by weight, Stearyl alcohol 0.5-1.5% by weight, Cyclopentasiloxane 0.8-1.2% by weight, Vitamin C 0.1-0.5% by weight, Madecassoside 0.05-0.15% by weight, Olive liquid 0.01-0.1% by weight, Sodium laureth sulfate 0.8-1.2% by weight, Cocamidomypa MIPA 0.1-0.5% by weight, brewer's yeast extract 0.05-0.15% by weight, acai berry extract 0.01-0.1% by weight, emollient oil 0.08-0.12% by weight, inchin extract 0.01-0.1% by weight, sansai extract 0.01-0.05% by weight, grapefruit seed extract 0.01-0.05% by weight, sodium alginate 0.01-0.05% by weight, pine bark extract 0.01-0.05% by weight, pine needle sprout extract 0.01-0.05% by weight, panthenol 0.04-0.08% by weight, salicylic acid It may also contain 0.01-0.05% by weight of Acid, 0.08-0.12% by weight of Polyquaternium-7, 0.07-0.13% by weight of Niacinamide, 0.1-0.3% by weight of Jojoba Seed Oil, 0.1-1% by weight of Behenyl Alcohol, 0.5-1.5% by weight of Myristyl Alcohol, 0.07-0.13% by weight of Cyclodextrin, 0.01-0.1% by weight of Fragrance, and the remainder being deionized water.

[0023] Specific details of other embodiments are included in the detailed description. [Effects of the Invention]

[0024] Skin-improving cosmetic compositions containing low-molecular-weight collagen, collagen water, or extracts, based on various embodiments of the technical concept of the present invention, exhibit excellent skin-soothing, moisturizing, and skin barrier-improving effects when low-molecular-weight collagen, collagen water, or collagen extracts are used as cosmetic compositions, thereby improving skin health.

[0025] Furthermore, skin-improving cosmetic compositions containing low-molecular-weight collagen, collagen water, or extracts, based on various embodiments of the technical concept of the present invention, can not only remove makeup but also effectively remove dead skin cells and waste products from pores, minimizing skin problems and irritation, and leaving dry, rough skin moist and smooth.

[0026] It is fully understood that various embodiments of the technical concept of the present invention can provide a variety of effects that are not specifically mentioned. [Brief explanation of the drawing]

[0027] [Figure 1] This graph shows the skin brightness improvement effect of the cosmetics produced in Examples 1, 2, and 3, and the cosmetics produced in Comparative Examples 1 and 2. [Figure 2] This graph shows the skin wrinkle-improving effects of the cosmetics produced in Examples 1, 2, and 3, and the cosmetics produced in Comparative Examples 1 and 2. [Modes for carrying out the invention]

[0028] The advantages and features of the present invention, and methods for achieving them, will become clear with reference to the embodiments described herein in detail. The present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, the embodiments presented herein are provided to ensure that the disclosed content is thorough and complete and that the idea of ​​the invention is fully conveyed to those skilled in the art.

[0029] The terminology used in this application is for the sole purpose of describing specific embodiments and is not intended to limit the invention. Singular expressions include plural expressions unless the context clearly indicates otherwise.

[0030] Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as they would be generally understood by a person of ordinary skill in the art to which this invention pertains. Terms as defined in commonly used dictionaries have a meaning consistent with their meaning in the context of the relevant art and should not be interpreted as ideal or overly formal unless explicitly defined herein.

[0031] The following describes in detail, with reference to preferred examples, a skin-improving cosmetic composition containing low-molecular-weight collagen, collagen water, or an extract, according to one embodiment of the technical concept of the present invention.

[0032] A skin-improving cosmetic composition containing low-molecular-weight collagen, collagen water, or an extract according to one embodiment of the technical concept of the present invention may contain at least one collagen selected from low-molecular-weight collagen powder, collagen water, or collagen extract.

[0033] Collagen, as mentioned above, is a structural protein found in most animals, and is particularly essential for the meat and connective tissue of mammals. Our bodies contain approximately 20% protein, of which 30% is collagen (LC. Junqueira and J. Carneiro 1983). It is also found in large quantities in the bones and skin of animals such as cows, pigs, chickens, and fish. Currently, collagen is used in a wide range of products, from health foods to cosmetics and pharmaceuticals.

[0034] The aforementioned collagen is generally classified into high molecular weight collagen (5,000 daltons or more) and low molecular weight collagen (1,500 daltons or less) according to its molecular weight. Low molecular weight collagen is known to have a relatively higher absorption rate in the body than high molecular weight collagen and is used in health functional foods, cosmetics, and over-the-counter medicines.

[0035] Collagen, the main protein that makes up the skin, is a fibrous protein that accounts for about 90% of the dermis. Along with elastin, it provides the tension and elasticity of the skin and protects the skin from ultraviolet rays, thus preventing wrinkles. It is known that collagen can be transmitted from the epidermis to the dermis layer when its molecular weight is at least 500 Da or less.

[0036] In muscle tissue, collagen acts as the main component of the endomysium. Collagen has a molecular weight of 300,000, and three α-chains with molecular weights of 100,000 come together to form a γ-chain, creating a right-handed helix with one turn every 40 residues. It has a triple helix structure with a diameter of 15 nm and a length of 300 nm. The α-chains are strongly bound together by some hydrogen bonds, and when microfibrils gather together, they become collagen fibrils through covalent cross-linking, and these then combine to form fibers.

[0037] The low molecular weight collagen powder may be collagen powder with a molecular weight of 200 to 300 daltons (Da), but the low molecular weight collagen powder may be collagen powder produced by the following method.

[0038] First, collagen may be prepared in order to produce low-molecular-weight collagen powder.

[0039] As the collagen, various types of animals containing collagen may be used. For example, as the animal collagen-containing material, at least one selected from the group consisting of fish, collagen extracted from fish scales, chicken foot collagen, cow foot collagen, and pig foot collagen may be used.

[0040] Next, the collagen may be sterilized.

[0041] The sterilization treatment may be carried out using known sterilization methods such as ultra-high temperature (UHT) sterilization or high temperature short-time sterilization (HTST). Specifically, the sterilization conditions using high temperature short-time sterilization (HTST) may involve sterilization at a temperature of 70-75°C for 10-20 seconds, and the sterilization conditions using ultra-high temperature (UHT) may involve sterilization at a temperature of 130-140°C for 2-10 seconds.

[0042] Next, the sterilized collagen may be enzymatically hydrolyzed to produce collagen hydrolysates.

[0043] The enzymatic decomposition may be carried out by mixing 80 to 120 parts by weight of the sterilized material, 20 to 40 parts by weight of proteolytic enzyme, and 150 to 250 parts by weight of purified water to produce a collagen enzyme mixture, and then heating the collagen enzyme mixture in a water bath at a temperature of 57 to 635°C for 1 to 5 hours. For example, at least one selected from the group consisting of aminopeptidase, flavozyme, protamax, protease, neutrase, and alcalase may be used as the proteolytic enzyme.

[0044] Specifically, the proteolytic enzyme may be purchased as a commercially available product, but for example, the proteolytic enzyme may be a mixture of alcalase (Novozymes, Bagsvaerd, Denmark) and protease-NP (Bioland, Asan, Korea) in a 1:1 weight ratio.

[0045] Next, the collagen enzyme hydrolysate may be decolorized and deodorized.

[0046] The collagen enzyme hydrolysate may be decolorized and deodorized to remove odor and produce a white, bleached collagen enzyme hydrolysate. For example, the decolorization and deodorization may be carried out by immersing the collagen enzyme hydrolysate in sodium hypochlorite for 10 to 60 minutes.

[0047] Next, the decolorized and deodorized collagen enzyme hydrolysate may be concentrated to produce a collagen concentrate.

[0048] The collagen concentrate may be produced by concentrating the decolorized and deodorized collagen enzyme hydrolysate using a known concentrator. However, since the method of concentration using such a concentrator is a known technique, a specific explanation of this will be omitted for the sake of convenience of explanation and clarity of the technical concept of the present invention.

[0049] Next, the collagen concentrate may be dried to produce collagen powder with a molecular weight of 200 to 300 daltons (Da).

[0050] For example, the collagen powder may be produced by spray-drying the collagen concentrate at a temperature of 160 to 180°C.

[0051] Furthermore, the collagen water used may be collagen water produced by the following method.

[0052] First, collagen may be prepared in order to produce the collagen water.

[0053] As the collagen, various types of animals containing collagen may be used. For example, as the animal collagen-containing material, at least one selected from the group consisting of fish, collagen extracted from fish scales, chicken foot collagen, cow foot collagen, and pig foot collagen may be used.

[0054] Next, the collagen may be sterilized.

[0055] The sterilization treatment may be carried out using known sterilization methods such as ultra-high temperature (UHT) sterilization or high temperature short-time sterilization (HTST). Specifically, the sterilization conditions using high temperature short-time sterilization (HTST) may involve sterilization at a temperature of 70-75°C for 10-20 seconds, and the sterilization conditions using ultra-high temperature (UHT) may involve sterilization at a temperature of 130-140°C for 2-10 seconds.

[0056] Next, the sterilized collagen may be distilled to produce a collagen distillation extract.

[0057] For example, the collagen distillation extract may be produced by adding 10 to 30 times (v / w) distilled water to the sterilized collagen, heating the mixture at 60 to 80°C for 10 to 50 minutes, then distilling the extract while heating at 100°C, and recovering the distillate at 30 to 40% of the total weight of the mixture.

[0058] Next, the collagen distillation extract may be filtered to produce collagen water.

[0059] For example, the collagen water may be produced by filtering the collagen distillation extract through a known filter to remove solid matter, thereby extracting the useful components of collagen.

[0060] Furthermore, the collagen extract used may be a collagen extract produced by the following method.

[0061] First, collagen may be prepared in order to produce the collagen extract.

[0062] As the collagen, various known types of animals containing collagen may be used. For example, as the animal collagen-containing material, at least one selected from the group consisting of fish, collagen extracted from fish scales, chicken foot collagen, cow foot collagen, and pig foot collagen may be used.

[0063] Next, the collagen may be sterilized.

[0064] The sterilization treatment may be carried out using known sterilization methods such as ultra-high temperature (UHT) sterilization or high temperature short-time sterilization (HTST). Specifically, the sterilization conditions using high temperature short-time sterilization (HTST) may involve sterilization at a temperature of 70-75°C for 10-20 seconds, and the sterilization conditions using ultra-high temperature (UHT) may involve sterilization at a temperature of 130-140°C for 2-10 seconds.

[0065] Next, the sterilized collagen may be subjected to hot water extraction to produce a collagen hot water extract.

[0066] For example, the collagen hot water extract may be produced by mixing 100 parts by weight of the total content of the sterilized collagen with 80 to 120 parts by weight of purified water, and then heating at a temperature of 90 to 100°C for 1 to 3 hours to perform hot water extraction.

[0067] Next, the collagen hot water extract may be filtered to produce a collagen extract.

[0068] For example, the collagen extract may be produced by filtering the collagen hot water extract through a known filter to remove solid matter, thereby extracting the oil-soluble components of collagen.

[0069] A skin-improving cosmetic composition containing low molecular weight collagen, collagen water, or extract according to one embodiment of the technical concept of the present invention includes the low molecular weight collagen powder, collagen water, collagen extract, caffeic acid, soybean-derived lysolecithin, herbal fermentation extract, coenzyme Q10, tocopherol acetate, butylene glycol, stearyltrimethylammonium chloride, L-aspartic acid, sorbitol, stearyl alcohol, cyclopentasiloxane, vitamin C, madecassoside, olive liquid, sodium laureth sulfate, cocamide MIPA, brewer's yeast extract, acai berry extract, emollient oil, inchin extract, sansai extract, grapefruit seed extract, sodium alginate, pine bark extract, pine needle sprout extract, panthenol, and salicylic acid. Contains Acid, Polyquaternium-7, Niacinamide, Jojoba Seed Oil, Behenyl Alcohol, Myristyl Alcohol, Cyclodextrin, Fragrance, and the remainder of deionized water.

[0070] Specifically, a skin-improving cosmetic composition containing low molecular weight collagen, collagen water, or extract according to one embodiment of the technical concept of the present invention comprises: 0.1-0.3% by weight of low molecular weight collagen powder, 0.5-1.5% by weight of collagen water, 0.1-1% by weight of collagen extract, 0.1-1% by weight of caffeic acid, 0.1-0.5% by weight of soy-derived lysolecithin, 0.5-1.5% by weight of herbal fermented extract, and coenzyme Q10. Q10) 0.08-0.12% by weight, Tocopherol acetate 0.1-0.3% by weight, Butylene glycol 0.5-1.5% by weight, Stearyltrimethylammonium chloride 0.1-0.3% by weight, L-Aspartic acid 0.08-0.12% by weight, Sorbitol 0.05-0.15% by weight, Stearyl alcohol 0.5-1.5% by weight, Cyclopentasiloxane 0.8-1.2% by weight, Vitamin C 0.1-0.5% by weight, Madecassoside 0.05-0.15% by weight, Olive liquid 0.01-0.1% by weight, Sodium laureth sulfate 0.8-1.2% by weight, Cocamidomypa MIPA 0.1-0.5% by weight, brewer's yeast extract 0.05-0.15% by weight, acai berry extract 0.01-0.1% by weight, emollient oil 0.08-0.12% by weight, inchin extract 0.01-0.1% by weight, sansai extract 0.01-0.05% by weight, grapefruit seed extract 0.01-0.05% by weight, sodium alginate 0.01-0.05% by weight, pine bark extract 0.01-0.05% by weight, pine needle sprout extract 0.01-0.05% by weight, panthenol 0.04-0.08% by weight, salicylic acid Acid) 0.01~0.05 wt%, Polyquaternium-7 0.08~0.12 wt%, Niacinamide 0.07~0.13 wt%, Jojoba Seed Oil 0.1~0.3 wt%, Behenyl Alcohol 0.1~1 wt%, Myristyl Alcohol 0.5~1.5 wt%, Cyclodextrin 0.07~0.13 wt%, Fragrance 0.01~0.It may contain 1% by weight and the remainder as deionized water.

[0071] Caffeic acid, as mentioned above, is an organic compound classified as hydroxycinnamic acid, consisting of a phenol group and an acrylic group. Caffeic acid has been found in all plants because it is an important intermediate in the biosynthesis of lignin, a major component of biomass. Caffeic acid has been shown to have potent antioxidant activity both in vitro and in vivo, and also possesses immunomodulatory and anti-inflammatory activity.

[0072] In the present invention, known caffeic acids may be used as the caffeic acid, but for example, a caffeic acid derivative with the structural formula shown in Chemical Formula 1 below may be used as the caffeic acid.

[0073] [ka]

[0074] In the above formula 1, R is C 4-6 The side chain is alkyl or cyclohexyl.

[0075] Specifically, the caffeic acid derivative represented by Formula 1 may be at least one selected from (E)-4-(3-(cyclohexyloxy)-3-oxoprof-1-en-1-yl)-1,2-phenylenebis(2,2-dimethylpropanoate) or (E)-4-(3-(cyclohexyloxy)-3-oxoprof-1-en-1-yl)-1,2-phenylenedicyclohexanecarboxylate.

[0076] The aforementioned soybean-derived lysolecithin is lysolecithin extracted from soybeans, and this lysolecithin functions as a transdermal permeation enhancer and emulsifier, and can contribute to increased absorption by the skin.

[0077] The lysolecithin mentioned above may be a known lysolecithin extracted from soybeans. Soybeans, one of Korea's traditional foods, are generally used as a source of high-quality protein and lipids. They have been used in a variety of applications, from fermented foods such as miso and soy sauce to non-fermented foods such as tofu, bean sprouts, and soy flour. Recently, they have begun to attract attention as a functional food that can prevent aging and lifestyle-related diseases, including skin whitening.

[0078] Numerous studies have been conducted on soybeans in relation to skin aging, skin whitening, and moisturizing, and the number of skin beauty products using soybeans being released is increasing. Korean Patent Publication No. 10-2004-0106893 discloses soybean yellow syrup extract and a functional cosmetic product for preventing skin aging containing it as an active ingredient, and Korean Patent Publication No. 10-2005-0079478 discloses cheonggukjang extract obtained by fermenting soybeans with Bacillus bacteria and a cosmetic composition containing it. An invention relating to a cosmetic composition using beans is a soybean fermentation extract obtained by fermenting soybeans with Bacillus bacteria and a cosmetic composition containing it (Registered Patent 10-0810006).

[0079] In the present invention, the lysolecithin may be lysolecithin produced by extraction from soybeans, but since the composition of the lysolecithin is a known technique, a specific explanation thereof will be omitted for the sake of convenience of explanation and clarity of the technical concept of the present invention.

[0080] The aforementioned herbal fermented extract may be produced by fermenting herbal materials and then extracting them, but the aforementioned herbal fermented extract may also be produced by the following method.

[0081] In other words, in order to produce the aforementioned herbal fermented extract, one may first prepare the herbal ingredients.

[0082] The aforementioned herbal medicine ingredients may include peony, mulberry leaves, Poria cocos, balloon flower, ginger, licorice, macadamia, black cumin seeds, chia seeds, moringa seeds, and safflower seeds. The aforementioned herbal medicine ingredients may be prepared in the following weight ratios: 10-20 parts by weight of peony, 1-10 parts by weight of mulberry leaves, 5-10 parts by weight of Poria cocos, 5-15 parts by weight of balloon flower, 10-20 parts by weight of ginger, 1-3 parts by weight of licorice, 1-5 parts by weight of macadamia, 1-5 parts by weight of black cumin seeds, 1-5 parts by weight of chia seeds, 1-5 parts by weight of moringa seeds, and 1-5 parts by weight of safflower seeds.

[0083] The aforementioned peony (Paeonia lactiflora Pall) belongs to the Paeoniaceae family and is classified into red and white varieties. Peonies are known to be widely distributed in a wide area including northeastern China (north of Jiangsu Province), Mongolia, the southeastern Far East of Asia, and Korea. Peonies contain large amounts of various sugars, mucilage, organic acids, and trace minerals, and are known to treat nutritional deficiencies and have astringent properties. In addition, peonies improve vascular function, strengthen the digestive organs, and suppress nerve pain. Furthermore, peonies are known to suppress gastric acid secretion, strengthen bladder motility, relieve abdominal pain, and have excellent antibacterial, anti-inflammatory, and antipyretic properties.

[0084] The aforementioned mulberry leaf is the leaf of a plant belonging to the genus Morus in the family Moraceae. It has been used as silkworm feed for thousands of years, and has also been used in folk medicine as a herbal ingredient. Recently, research into its beneficial nutrients and physiologically active components has been progressing, bringing it into the spotlight as a functional food.

[0085] The mulberry leaves mentioned above contain water, carbohydrates, protein (25-35%), and 25 types of amino acids. Of these, serine and tyrosine, which are components that help improve cerebral blood circulation and prevent senile dementia, are present at levels of 1.2% and 0.8%, respectively. The lipid content is about 35%, with most of the fatty acids being unsaturated fatty acids. They also contain 0.8% plant sterols, which are known to reduce blood cholesterol levels.

[0086] Furthermore, mulberry leaves contain high levels of glutamic acid and aspartic acid, and compared to green tea, they have significantly higher levels of minerals such as calcium, iron, potassium, and dietary fiber. The physiological activities of mulberry leaves include blood lipid suppression, blood glucose strengthening, heavy metal adsorption inhibition and detoxification, and antioxidant effects. Components that have been identified as having physiological activity include GABA, which lowers blood pressure; rutin, which strengthens capillary blood vessels and particularly prevents cerebral hemorrhage; and flavonoid compounds that have excellent antioxidant, diuretic, and anti-capillary permeability effects.

[0087] The aforementioned "Tu Fu Ling" refers to the dried rhizome of Smilax china L., a plant belonging to the Smilacaceae family. Smilax china grows in all mountainous areas south of central Korea. The rhizomes are dug up in autumn and spring, washed with water, cut into appropriate lengths, and sun-dried for use as a medicinal ingredient. It is known to act on the stomach and liver meridians, reducing fever, eliminating dampness, and detoxifying. It has also been reported to be used for joint pain, syphilis, bead ulcers, sores, malignant tumors, and mercury poisoning.

[0088] The physiological activities of Smilax china include promoting the secretion of growth hormone (Registered Patent No. 10-0521845, Registered Patent No. 10-0498277), treating inflammatory diseases (Registered Patent No. 10-1093730), preventing vascular diseases (Registered Patent No. 10-1156636), skin whitening (Registered Patent No. 10-1086669), improving wrinkles and alleviating skin damage (Registered Patent No. 10-1086225), reducing heavy metals in the body (Korean Journal of Food and Nutrition, Vol. 24, No. 2, 233-238, 2011), antioxidant and antibacterial activity of Smilax china leaf extract (Korean Journal of Food Preservation, Vol. 18, No. 5, 764-772, 2011), and antifungal activity (Korean J. of It has been reported to have a wide variety of pharmacological effects, including protection of cranial nerves or prevention of degenerative brain diseases (Registered Patent No. 10-0779179), and preventive or therapeutic effects on obesity, hyperlipidemia, or fatty liver (Registered Patent No. 10-1225486). (Pharmacology, Vol. 9, No. 2, 1973)

[0089] Platycodon grandiflorum, also known as balloon flower (Platycodon grandiflorum), grows widely in mountainous regions of Korea, Japan, and China. It is used as a medicine in traditional Chinese medicine and is also widely consumed as a general food. It contains triterpenoid saponins, carbohydrates, and fiber. Animal studies have shown that these saponins have various effects, including antitussive, expectorant, central nervous system depressant, anti-inflammatory, anti-ulcer, and anti-gastric acid secretion inhibitory effects, blood glucose-lowering effects that dilate blood vessels and lower blood pressure, cholesterol metabolism-improving effects, antioxidant and anti-cancer effects. Platycodon grandiflorum has such diverse pharmacological effects that it is included in 287 traditional Chinese medicine prescriptions in the Dongui Bogam and 49 in the Fangyao Hebian.

[0090] The aforementioned balloon flower is not only good for coughs, colds, and asthma, but it also suppresses melanin production, making the skin whiter and smoother, eliminates waste products, promotes blood circulation, and has anti-inflammatory properties that make it effective in treating acne.

[0091] Furthermore, the roots of the balloon flower contain approximately 2% saponins, which have hemolytic properties, relieve coughs and phlegm, reduce inflammation, lower blood sugar, and lower cholesterol. They also contain a large amount of calcium, which is good for preventing osteoporosis and promoting bone health in growing children.

[0092] The aforementioned ginger (Zingiber officinale) is a perennial plant classified in the genus Zingiber of the family Zingiberaceae. Its rhizome is used in traditional Chinese medicine and has a pungent and bitter taste. The aroma and pungent taste of ginger are known to be due to a component called gingerol, which is known for its excellent antioxidant and anti-inflammatory properties.

[0093] The aforementioned Glycyrrhiza uralensis is a perennial herb belonging to the legume family. Its reddish-brown roots are sweet and are used in a variety of ways, including as food and a medicinal sweetener. Its main components include triterpene saponins and flavonoids, and it is known to exhibit various physiological activities such as anti-inflammatory, antibacterial, antioxidant, antithrombotic, antidiabetic, and antiviral properties.

[0094] The aforementioned macadamia fruit consists of 75% natural edible oils, 9% protein, 9% carbohydrates, and 2% dietary fiber. The remainder contains calcium, phosphorus, sodium, vitamins E, B1, B2, niacin, and iron, which are essential elements for human nutrition. It also contains selenium, an essential trace element in the body known to have anti-cancer effects, as well as effects on delaying the onset of AIDS and promoting growth in children.

[0095] The vitamins and unsaturated fatty acids contained in macadamia nuts give dry skin a radiant glow, improve blood circulation, and enhance the vitality of skin cells. Furthermore, the presence of unsaturated fatty acids, proteins, and minerals protects the skin from free radicals, providing anti-aging effects and contributing to the maintenance of smooth and healthy skin.

[0096] The aforementioned black cumin seed (Citrus Junos Seed) is also called the black seed of Nigella sativa or simply black seed. It is a rare plant belonging to the Ranunculaceae family, native to the Middle East, Southwest Asia, or Southeast Asia, and has been used as a medicinal herb for over 2,000 years. It is actually composed of components such as thymoquinone, thymohydroquinone, nigellimine-N-oxide, nigellicine, nigellidine, nigellone, dithymoquinone, thymol, arvacrol, 6-methoxycoumarin, 7-hydroxy-coumarin, oxycoumarin, alpha-hedrin, sterylglucoside, tannins, flavinoids, essential fatty acids, essential amino acids, ascorbic acid, iron, and calcium.

[0097] The aforementioned thymoquinone component exhibits excellent efficacy in treating fungal infections and is used to treat athlete's foot, mycoses, and fungal vaginitis. It is also known to alleviate psoriasis and acne, and in the pharmaceutical field, it is known to be useful in improving immune disorders, asthma, and allergies. It may also be used for various inflammatory and eczema-like skin conditions.

[0098] Chia seeds are a treasure trove of nutrients, rich in omega-3 fatty acids and containing a balanced amount of iron, potassium, calcium, magnesium, and dietary fiber. They are often called a dream food for dieters because they provide a feeling of fullness, reduce the amount of other foods consumed, and have many benefits, such as removing impacted stool from the intestines, due to their composition of water-soluble fiber.

[0099] In other words, chia seeds are known to be the food with the highest omega-3 fatty acid content, richer in calcium than milk, twice as rich in potassium as bananas, and more than three times as rich in iron as spinach.

[0100] Chia seeds, when placed in water, turn into a jelly-like substance and increase in volume by about 10 times due to their gelatinous properties. This provides a feeling of fullness, and they are particularly rich in nutrients, making them effective in supplementing nutrients that may be lacking during dieting. They also break down body fat and prevent fat accumulation, making them a popular diet food. Furthermore, they are rich in dietary fiber, which stimulates bowel movements and helps improve constipation. They also have antioxidant properties, are good for anti-aging and skin beauty, and help improve blood circulation, making them a food with many health benefits.

[0101] Moringa seeds are the seeds of the Moringa plant, which belongs to the Moringaceae family and Moringa genus. Its scientific name is Moringa Oleifera (also known as Moringa Pterygosperma), and it is also called Murunggai in Tamil, from which its scientific name originates. Moringa is a legume plant, a tropical tree whose entire tree, including its leaves and fruits, is edible, and it contains over 90 nutrients, including large amounts of amino acids, minerals, and vitamins.

[0102] In particular, the water-soluble proteins contained in moringa seeds play a role in purifying pollutants in water. When these water-soluble proteins are applied to the skin, they exhibit a detoxifying effect, purifying skin pollutants and cleansing the skin. Furthermore, they contain large amounts of carotenoid components such as beta-carotene and lutein, as well as vitamins and inorganic components, which brighten and improve dull skin, resulting in a whitening effect.

[0103] Safflower seeds contain linoleic acid, an unsaturated fatty acid, as well as inorganic substances such as mineral elements including potassium, calcium, magnesium, gold, aluminum, bromine, copper, iron, manganese, rubidium, and zinc, and components such as carthamine and chalconic acid glycosides. In particular, safflower sprouts contain a large amount of luteolin, a sulfated substance, and it has been revealed that it has medicinal effects that promote metabolism in our bodies or treat various diseases, leading to a dramatic increase in consumption.

[0104] Next, the prepared herbal ingredients may be mixed with a microbial culture solution and then fermented to produce a fermented herbal ingredient product.

[0105] For example, the fermented herbal medicine material may be produced by mixing the prepared herbal medicine material with microbial culture solution in a weight ratio of 10 to 30 parts by weight per 100 parts by weight of the total herbal medicine material, and then fermenting the mixture of herbal medicine material and microbial culture solution at a temperature of 38 to 42°C for 10 to 20 days.

[0106] The microbial culture medium may be a microbial culture medium prepared by the following method.

[0107] For example, in order to produce the microbial culture medium, the culture medium may first be prepared and then sterilized.

[0108] The culture medium may be a conventional culture medium used for culturing microorganisms, or it may be prepared by sterilizing the prepared culture medium at a temperature of 80-90°C for 40-80 minutes and then cooling it at a temperature of 30-35°C.

[0109] Since the culture medium used to cultivate the aforementioned microorganisms has a known configuration, a specific explanation thereof will be omitted for the sake of convenience of explanation and clarity of the technical concept of the present invention.

[0110] Next, a culture medium may be prepared by mixing the culture medium with a culture solution containing nutrients necessary for the cultivation of microorganisms.

[0111] The culture medium may be produced by mixing a culture solution into the medium. For example, the culture solution contains glucose, dextrin, glycerin, carrageenan (C 24 H 36 O 25 S2), lysine (C6H 14 N2O2), potassium chloride (KCl), sodium citrate, ethylene glycol (CH2OHCH2OH), magnesium chloride, peptone, malt extract, and pyridoxine hydrochloride (C9H 11 NO3HCl).

[0112] Specifically, the culture solution contains, per 1 liter (L) of purified water, 65 g / L of the glucose, 25 g / L of dextrin, 20 g / L of glycerin, 20 g / L of carrageenan (C 24 H 36 O 25 S2), 15 g / L of lysine (C6H 14 N2O2), 13 g / L of potassium chloride (KCl), 8 g / L of sodium citrate, 8 g / L of ethylene glycol (CH2OHCH2OH), 3 g / L of magnesium chloride, 5 g / L of peptone, 5 g / L of malt extract, and 1 g / L of pyridoxine hydrochloride (C9H 11 NO3HCl), and the culture medium may be produced by mixing at a weight ratio of 80 to 120 parts by weight of the culture solution with respect to 100 parts by weight of the total content of the medium.

[0113] The carrageenan is a polysaccharide mixture which is a viscous polymer electrolyte extracted from natural seaweeds such as Rhodophyceae Calss and Irish moss, and has been widely used as an emulsifier, dispersant, stabilizer, swelling agent, thickening agent, binder, crystallization inhibitor, etc.

[0114] Furthermore, the carrageenan used is a complex polysaccharide mainly composed of galactose and anhydrogalactose, and is a sodium ester substance that is a carrageenan copolymer with a molecular weight (Mw) of 200,000 to 400,000.

[0115] Lysine is an amino acid and may be used as an additive in food or feed, but in the present invention, lysine may be used to enhance the external adaptability of microorganisms.

[0116] The potassium chloride is used to increase the solubility of the composition constituting the culture medium; the sodium citrate is used as an electrolyte chloride; the ethylene glycol is used to increase the nutrient absorption rate of microorganisms; the peptone supplies proteins necessary for the growth of microorganisms as a nitrogen source that supplies proteins; the malt extract contains various minerals, B vitamins, and amino acids and is beneficial for the growth of microorganisms; and the pyridoxine hydrochloride may be used as a vitamin to increase the activity of microorganisms.

[0117] Next, a strain of microorganism may be inoculated into the culture medium.

[0118] The inoculation may be carried out by inoculating culture media with strains of microorganisms consisting of lactic acid bacteria, Bacillus subtilis, and yeast, but as the lactic acid bacteria, at least one selected from the group consisting of strains of the genera Lactobacillus, Lactococcus, Leuconostoc, Pediococcus, and Bifidobacterium may be used.

[0119] For example, a strain of the genus Lactobacillus may be used as the lactic acid bacterium, but at least one strain selected from the group consisting of Lactobacillus plantarum, Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus sakei, Lactobacillus casei, and Lactobacillus brevis may be used, and preferably, Lactobacillus plantarum may be used as the lactic acid bacterium.

[0120] Furthermore, the aforementioned yeast refers to microorganisms used to make bread, beer, wine, etc., and is a group of molds or mushrooms but lacks hyphae and does not possess photosynthetic ability or motility; it is a single-celled organism. Preferably, Saccharomyces cerevisiae may be used as the yeast.

[0121] Furthermore, the Bacillus subtilis may be at least one selected from the group consisting of Bacillus subtilis, Bacillus subtilis spizepiai, Bacillus Velezensis, Bacillus megaterium, Bacillus licheniformis, and Bacillus pumilus, but preferably, Bacillus Velezensis may be used as the Bacillus subtilis.

[0122] Furthermore, the inoculation amount of the aforementioned microbial strain is determined by the initial number of bacteria in the inoculation, which is 1 × 10⁻⁶.6 ~1 × 10 8 CFU / mL may also be acceptable.

[0123] Next, the microorganisms may be cultured in a culture medium inoculated with the aforementioned microbial strain, and then the cultured microorganisms may be mixed to produce a mixed culture solution.

[0124] Specifically, a lactic acid bacteria culture solution, a Bacillus subtilis culture solution, and a yeast culture solution may be produced by culturing culture media inoculated with lactic acid bacteria, Bacillus subtilis, and yeast, respectively, at a temperature of 38-42°C for 20-40 hours, and then mixing the lactic acid bacteria culture solution, Bacillus subtilis culture solution, and yeast culture solution in a weight ratio of 1:1:1 to produce a mixed culture solution.

[0125] Next, the mixed culture solution may be centrifuged to obtain the lower layer to produce a microbial culture solution.

[0126] For example, the microbial culture medium may be produced by centrifuging the mixed culture medium at 8,000xg to 12,000xg to obtain the lower layer. However, since the configuration of centrifuging a mixed culture medium using a known centrifuge is a known technique, a specific explanation of this will be omitted for the sake of convenience of explanation and clarity of the technical concept of the present invention.

[0127] Next, the fermented herbal medicine material may be dried to produce a dried fermented herbal medicine material.

[0128] By drying the fermented herbal material to gradually remove moisture, it is possible to prevent changes in the physical properties of the fermented herbal material and maximize the content of the active ingredients. For example, the dried fermented herbal material may be produced by drying the fermented herbal material at a temperature of 50-60°C for 1-5 days.

[0129] Next, the fermented and dried product of the aforementioned herbal medicine material may be extracted to produce a herbal medicine fermentation extract.

[0130] The aforementioned herbal fermentation extract may be produced by extracting the fermented and dried herbal material using a known extraction method. For example, the extraction of the fermented and dried herbal material may be performed using various known extraction methods such as hot water extraction, organic solvent extraction, ultrasonic extraction, or supercritical fluid extraction. Since the composition of the extraction is a known technique, a specific explanation will be omitted for the sake of clarity of this explanation and the technical concept of the present invention.

[0131] Coenzyme Q10 is well known to be involved in the synthesis of adenosine triphosphate (ATP) in mitochondria within cells. Human skin is generally exposed to a lot of environmental oxidative stress, which reduces the content of coenzyme Q10 in the skin and causes various problems such as photoaging. However, it is known that supplying coenzyme Q10 to the skin can prevent the decrease in content due to oxidative stress, and as a result is effective in slowing down aging.

[0132] The aforementioned tocopherol acetate is an antioxidant that contributes to primary defense against lipid peroxidation components and can play a role in protecting cells from oxidative damage.

[0133] Tocopherol acetate has antioxidant properties in the body, scavenging reactive oxygen species and suppressing the generation of free radicals and lipid peroxides. Tocopherol acetate itself is unstable, so its derivatives are often used, with vitamin acetate and vitamin palmitate being the most commonly used.

[0134] The aforementioned butylene glycol, also known as 1,3-butanediol as an aliphatic diol, is a viscous liquid with almost no odor, a sweet taste, and hygroscopic properties. It is soluble in water and ethanol, and is known to have excellent antibacterial properties and moderate moisturizing effects, as well as a moisturizing effect.

[0135] The aforementioned vitamin C is called L-ascorbic acid (Asc). This vitamin C has antioxidant properties, preventing cholesterol oxidation, maintaining blood vessels, and increasing interferon production. It is known to have an immune effect during the initial stages of influenza virus infection, as well as being effective in preventing and alleviating cold symptoms. In addition, vitamin C has the effect of eliminating lead and mercury accumulated in the body.

[0136] The madecassoside component is a substance isolated from Centella asiatica. Centella asiatica is a small herbaceous plant belonging to the Umbelliferae family that grows wild in tropical, shady regions at an ideal altitude of 600-800 meters above sea level. It is known to contain asiaticoside, madecassoside, asiatic acid, and madecassic acid components. Madecassoside plays a role in soothing the skin, strengthening the skin barrier, and aiding tissue recovery.

[0137] The aforementioned Olive Liquid is a solubilizer extracted from olive oil. Unlike other emulsifiers, it has its own moisturizing properties and is effective for atopic dermatitis and sensitive skin.

[0138] The aforementioned sodium laureth sulfate is a known surfactant, and those skilled in the art may easily purchase and use it commercially.

[0139] The aforementioned cocamide MIPA is mainly a mixture of coconut acid and isopropanolamide, and possesses surfactant properties, and can also function as a thickener, foaming agent, and binder.

[0140] The aforementioned hydrolyzed collagen can be used for skin conditioning and may be hydrolyzed animal and fish collagen obtained by acid, enzyme, or other hydrolysis methods. It is expected to promote the regeneration of skin fibers, regulate the skin's pH, activate metabolism, and provide moisturizing effects.

[0141] The aforementioned brewer's yeast extract is extracted from brewer's yeast, which is used in brewers' brewing, alcoholic beverage production, and bread production. Brewer's yeast is one of the yeast species necessary for making beer. It is a dried, non-fermentable yeast of the genus Saccharomyces, isolated after the fermentation of the wort is complete and the beer has been filtered during the beer production process. It contains about 45% protein and is high in B vitamins and phosphorus. Furthermore, this strain produces high concentrations of ethanol and has the characteristic of being able to withstand high-concentration ethanol environments. Brewer's yeast contains large amounts of protein, nucleic acid components, carbohydrates, minerals, and vitamins.

[0142] The beer yeast may be yeast obtained during the beer fermentation process. The beer yeast may include at least one Saccharomyces bayanus yeast from among Saccharomyces cerevisiae, Saccharomyces carlsbergenesis, and Saccharomyces bayanus.

[0143] The aforementioned acai berry extract is extracted from the acai berry, the fruit of the acai palm, which belongs to the Arecaceae family and grows in the tropical rainforest region of the Amazon River in northern Brazil. The acai berry extract is rich in amino acids such as aspartic acid and glutamic acid, as well as oleic acid, unsaturated fatty acids, vitamin A, vitamin C, calcium, iron, and other minerals. In particular, the skin of the acai berry contains functional components such as anthocyanin, a flavonoid-type water-soluble pigment, and beta-sitosterol, a type of phytosterol, which are known to have the effect of removing harmful reactive oxygen species.

[0144] The emollient oil may be used to provide a moisturizing effect to the skin and to dissolve oil-soluble physiologically active ingredients, but for example, the emollient oil may be at least one oil selected from the group consisting of squalene, trioctanoate, cetearyloctanoate cyclomethicone, and dimethicone.

[0145] The aforementioned inchin extract is extracted from inchin, and inchin (Artemisiae Capillaris Herba) refers to the dried above-ground parts of Artemisia capillaris Thunberg, a plant in the Asteraceae family. Inchin is used for jaundice caused by damp heat, that is, for symptoms such as fever, general yellowing of the body, and red, reduced urine in acute hepatitis.

[0146] Furthermore, incin is used for chronic hepatitis, cirrhosis, liver cancer, cholecystitis, and gallstones, as well as for skin diseases such as eczema, scabies, scabies, and rubella, and for symptoms of severe fever and madness caused by epidemics. The pharmacological effects of incin include bile secretion promoting, antipyretic, diuretic, antimicrobial, and inhibitory effects on experimentally induced ascites cancer cells.

[0147] The aforementioned three vegetable extract is extracted from three vegetables, which generally have sweet, bitter, and spicy flavors and are sometimes called "three vegetables," and sometimes "carrot vegetables" because they have a carrot-like taste. These three vegetables are crops that propagate vegetatively, like garlic.

[0148] The main component of the aforementioned three vegetables, dietary sulfur, contains 60 times more saponins than wild ginseng, and is rich in nitrogen and fiber along with minerals such as calcium, iron, zinc, and manganese. It has various beneficial effects, including the formation of bones and teeth, blood production and vital blood function, increased vitality, regulation of bodily balance, prevention of high blood pressure, arteriosclerosis, constipation, and promotion of bowel movements.

[0149] The role of dietary sulfur is closely related to improving liver function and detoxification, including alpha and gamma sulfur for psychotropic diseases (dementia, intellectual disability), beta sulfur for cancer or chronic diseases (diabetes, hypertension, hypotension), delta sulfur for skin diseases (Agent Orange, psoriasis, atopic allergies), and lambda and mu sulfur for strengthening the immune system. While ginseng, red ginseng, mountain ginseng, and sen ginseng contain only beta sulfur, it is known that the three vegetables contain all six components.

[0150] The aforementioned grapefruit seed extract is extracted from grapefruit seeds, which contain ascorbic acid, sterols, dehydroascorbic acid, tocopherol, palmitic acid, and other active ingredients. These various active ingredients are known to weaken the cell membrane-related enzyme activity and permeability of microorganisms, thereby inhibiting cellular respiration and inhibiting microbial growth.

[0151] In particular, the aforementioned grapefruit seeds contain naringin and citral, which are bioflavonoids, as their main components, and therefore not only have excellent antibacterial and antioxidant properties, but also exhibit excellent antibacterial activity against mold as well as Gram-positive and Gram-negative bacteria.

[0152] The aforementioned sodium alginate, with the chemical formula (C6H7O6Na)n, is a white to pale yellow fibrous, granular, or powder substance with almost no odor and no taste. It is a bio-derived substance that increases the stickiness and viscosity of food, enhances emulsification stability, and improves the physical properties and texture of food. It is used as a stabilizer for ice cream, a thickener for ketchup, mayonnaise, and sauces, a clarifying agent for sake, a gelling agent, an emulsifier, and a thickener.

[0153] Sodium alginate is a high-molecular-weight substance, and various grades of the product exist through the purification process. When dissolved in water, it becomes viscous, and a 1% aqueous solution has a pH of 6-8, making it nearly neutral. Sodium alginate hardens when it comes into contact with sodium chloride (NaCl) or at low pH levels. Due to these properties, sodium alginate has been used as a thickening agent and for other purposes.

[0154] The sodium alginate may be produced by extraction from Sargassum fusiforme. For example, the extraction of sodium alginate may be carried out by the method of Calumpong et al (1999). Specifically, the sodium alginate may be produced by adding 800 mL of 2% formaldehyde to 25 g of Sargassum fusiforme, performing a primary pretreatment at room temperature for 24 hours, washing the Sargassum fusiforme with water, performing a secondary pretreatment in 800 mL of 0.2 M hydrochloric acid (HCl) for 24 hours, then immersing the pretreated Sargassum fusiforme in 1 L of 2% sodium carbonate (Na2CO3) and extracting at a temperature of 60-80°C for 1-3 hours, centrifuging the extracted solution at 3200 rpm for 25 minutes, precipitation to three times the volume of 95% ethanol (Ethanol), and finally drying at 60°C for 50 hours.

[0155] The aforementioned Moutan Radicis Cortex extract is manufactured by extracting from Moutan Radicis Cortex, which is an important traditional Chinese medicine that has been used since ancient times. It is a deciduous shrub belonging to the Ranunculaceae family and the name refers to "the bark of the peony root." Its main components are paeonoside (paeonol glucoside), paeonolide (paeonol-rhamnoglucoside), and paeonol. Paeonoside decomposes during storage to produce sugar and paeonol. Paeonol has antibacterial properties and has been shown to inhibit the growth of E. coli, Staphylococcus aureus, Streptococcus, Bacillus subtilis, and other bacteria when diluted 1,500 to 2,000 times in a test tube. Furthermore, it contains components such as paeoniflorin, oxypaeoniflorin, benzoylpaeoniflorin, paeonolide tannin, procyanidin B1, benzoyloxypaeoniflorin, paeonin, astragalin, and pelargonin. Histochemical studies have confirmed that these components are present not only in the root bark but also in the woody core. Paeoniflorin has an inhibitory effect on platelet aggregation and reduces fibrinogen. Benzoylpaeoniflorin and benzoyloxypaeoniflorin have an inhibitory effect on histamine release in mast cells, and paeonolide tannin has an antiviral effect. The anti-inflammatory effect is presumed to be due to monoterpene glycosides. Makitanpi has a cool nature and is used in traditional Chinese medicine as an anti-inflammatory and blood-stasis-removing agent. Its medicinal effects include treating inflammation of the vascular system of the lower abdominal organs, pain due to congestion, fever, suppuration, and bleeding. In particular, in gynecology, it has anti-inflammatory, analgesic, and antispasmodic effects on menstrual irregularities, inflammation and congestion of the uterus and adnexa, and traction pain, and is also applied to hemorrhoids and appendicitis.

[0156] The aforementioned pine needle sprout extract is manufactured by extracting from pine needle sprouts, which are the leaves of the pine tree (Pinus densiflora Siebold et Zuccarini), a tree with a long history of use among the Korean people in Northeast Asia, including Korea, China, and Japan. Fresh pine needles contain large amounts of ascorbic acid, vitamins A, B, and K, bitter substances, flavonoids, anthocyanins, 7-12% resin, about 5% tannins, carbohydrates, and essential oils.

[0157] In addition, the entire pine tree contains alcohol, esters, and other components that help eliminate waste products from the body and promote metabolism, as well as phenolic compounds, quinine, terpentin, vitamins A and C, chlorophyll, glycogenin, and abietic acid. It is also rich in iron, and 24 types of amino acids are found in red pine needles, with 19 types of amino acids consisting of proteins being identified.

[0158] Pine needles are both a source and a raw material for vitamin C, and because they are a much darker green color, they also provide abundant carotene. In the case of pine needles, the pharmacological value refers to the general effects of terpenes, phenolic compounds, and tannins. In addition, they are beneficial for hypertension, stroke, diabetes, dementia and aging prevention, anti-cancer effects, liver and stomach health, constipation and anemia, chronic alcoholism and hangover relief, nicotine removal, skin beautification and improved constitution, weight loss, male stamina, and when the head feels heavy and vision is blurred due to various stresses, excessive smoking, and drinking, they are especially good for students and exam takers who want to clear their heads.

[0159] The aforementioned glasswort extract is manufactured by extracting glasswort (Salicornia spp.), which is an annual plant belonging to the Chenopodiaceae family and is a representative edible and medicinal salt-tolerant plant that grows wild in Korea. Glasswort has long been used as a folk remedy for vision loss, indigestion, gastrointestinal diseases, hepatitis, kidney disease, etc., and has been scientifically proven to have antioxidant, anti-diabetic, anti-cancer, anti-hypertensive, anti-hyperlipidemia, and skin whitening effects.

[0160] The aforementioned jojoba seed oil is extracted by pressing the seeds of the jojoba plant, which grows in desert regions, and is extracted as a liquid wax. The jojoba seed oil is well absorbed by the skin, does not clog pores, delivers moisture deep into the skin, and is effective in balancing the skin. The jojoba seed oil is good for dry and aging skin, and exhibits excellent moisturizing effects by forming a moisture film on the skin to help prevent moisture loss.

[0161] The present invention will be described in more detail below with reference to examples of skin-improving cosmetic compositions containing low-molecular-weight collagen, collagen water, or extract, according to one embodiment of the technical concept of the present invention.

[0162] <Example 1> Low molecular weight collagen powder 0.2% by weight, collagen water 1% by weight, collagen extract 0.5% by weight, caffeic acid 0.5% by weight, soy-derived lysolecithin 0.3% by weight, herbal fermented extract 1% by weight, coenzyme Q10 0.1% by weight, tocopherol acetate 0.2% by weight, butylene glycol 1% by weight, stearyltrimethylammonium chloride 0.2% by weight, L-aspartic acid 0.1% by weight, sorbitol 0.1% by weight, stearyl alcohol 1% by weight, cyclopentasiloxane 1% by weight, vitamin C 0.3% by weight, madecassoside 0.1% by weight, olive liquid 0.05% by weight, sodium laureth sulfate 1% by weight, cocamidomirapa MIPA 0.3% by weight, Brewer's yeast extract 0.1% by weight, Acai berry extract 0.05% by weight, Emollient oil 0.1% by weight, Inch extract 0.05% by weight, Sansai extract 0.03% by weight, Grapefruit seed extract 0.03% by weight, Sodium alginate 0.03% by weight, Pine bark extract 0.03% by weight, Pine needle sprout extract 0.03% by weight, Panthenol 0.06% by weight, Salicylic acid 0.03% by weight, Polyquaternium-7 0.1% by weight, Niacinamide 0.1% by weight, Jojoba seed oil 0.2% by weight, Behenyl alcohol 0.5% by weight, Myristyl alcohol A cosmetic product was prepared by mixing 1% by weight of alcohol, 0.1% by weight of cyclodextrin, 0.05% by weight of fragrance, and the remaining amount of deionized water.

[0163] <Example 2> Low molecular weight collagen powder 0.25% by weight, collagen water 0.7% by weight, collagen extract 0.9% by weight, caffeic acid 0.3% by weight, soy-derived lysolecithin 0.4% by weight, herbal fermented extract 0.8% by weight, coenzyme Q10 0.11% by weight, tocopherol acetate 0.15% by weight, butylene glycol 1.3% by weight, stearyltrimethylammonium chloride 0.15% by weight, L-aspartic acid 0.09% by weight, sorbitol 0.13% by weight, stearyl alcohol 0.7% by weight, cyclopentasiloxane 1.1% by weight, vitamin C 0.2% by weight, madecassoside 0.13% by weight, olive liquid 0.03% by weight, sodium laureth sulfate 1.1% by weight, cocamidomirapa MIPA 0.2% by weight, Brewer's yeast extract 0.14% by weight, Acai berry extract 0.03% by weight, Emollient oil 0.11% by weight, Inchin extract 0.03% by weight, Sansai extract 0.04% by weight, Grapefruit seed extract 0.02% by weight, Sodium alginate 0.04% by weight, Pine bark extract 0.02% by weight, Pine needle sprout extract 0.04% by weight, Panthenol 0.05% by weight, Salicylic acid 0.04% by weight, Polyquaternium-7 0.09% by weight, Niacinamide 0.12% by weight, Jojoba seed oil 0.15% by weight, Behenyl alcohol 0.9% by weight, Myristyl alcohol A cosmetic product was prepared by mixing 0.7% by weight of alcohol, 0.12% by weight of cyclodextrin, 0.05% by weight of fragrance, and the remaining amount of deionized water.

[0164] <Example 3> Low molecular weight collagen powder 0.15% by weight, collagen water 1.4% by weight, collagen extract 0.3% by weight, caffeic acid 0.8% by weight, soy-derived lysolecithin 0.4% by weight, herbal fermented extract 1.3% by weight, coenzyme Q10 0.09% by weight, tocopherol acetate 0.25% by weight, butylene glycol 0.7% by weight, stearyltrimethylammonium chloride 0.25% by weight, L-aspartic acid 0.11% by weight, sorbitol 0.09% by weight, stearyl alcohol 1.3% by weight, cyclopentasiloxane 0.9% by weight, vitamin C 0.4% by weight, madecassoside 0.07% by weight, olive liquid 0.08% by weight, sodium laureth sulfate 0.9% by weight, cocamidomirapa MIPA 0.4% by weight, Brewer's yeast extract 0.07% by weight, Acai berry extract 0.08% by weight, Emollient oil 0.09% by weight, Inchin extract 0.08% by weight, Sansai extract 0.02% by weight, Grapefruit seed extract 0.04% by weight, Sodium alginate 0.02% by weight, Pine bark extract 0.04% by weight, Pine needle sprout extract 0.02% by weight, Panthenol 0.07% by weight, Salicylic acid 0.02% by weight, Polyquaternium-7 0.11% by weight, Niacinamide 0.08% by weight, Jojoba seed oil 0.25% by weight, Behenyl alcohol 0.3% by weight, Myristyl alcohol A cosmetic product was prepared by mixing 1.3% by weight of alcohol, 0.09% by weight of cyclodextrin, 0.05% by weight of fragrance, and the remaining amount of deionized water.

[0165] <Comparative Example 1> Coenzyme Q10 0.5% by weight, Tocopherol acetate 0.5% by weight, Butylene glycol 1.3% by weight, Stearyltrimethylammonium chloride 0.2% by weight, L-aspartic acid 0.5% by weight, Sorbitol 0.2% by weight, Stearyl alcohol 1% by weight, Cyclopentasiloxane 1.5% by weight, Vitamin C 0.8% by weight, Madecassoside 0.3% by weight, Olive liquid 0.08% by weight, Sodium laureth sulfate 1.2% by weight, Cocamidomypa A cosmetic was prepared by mixing MIPA 0.5% by weight, brewer's yeast extract 0.3% by weight, acai berry extract 0.1% by weight, emollient oil 0.3% by weight, grapefruit seed extract 0.05% by weight, basil bark extract 0.03% by weight, panthenol 0.1% by weight, salicylic acid 0.03% by weight, polyquaternium-7 0.15% by weight, niacinamide 0.18% by weight, jojoba seed oil 0.8% by weight, behenyl alcohol 0.9% by weight, myristyl alcohol 3% by weight, cyclodextrin 0.5% by weight, fragrance 0.05% by weight, and the remainder of deionized water.

[0166] <Comparative Example 2> Collagen extract 1.5% by weight, Caffeic acid 0.5% by weight, Coenzyme Q10 0.5% by weight, Tocopherol acetate 0.6% by weight, Butylene glycol 1.5% by weight, Sorbitol 0.5% by weight, Stearyl alcohol 1.5% by weight, Cyclopentasiloxane 2% by weight, Vitamin C 0.3% by weight, Madecassoside 0.5% by weight, Olive liquid 0.05% by weight, Acai berry extract 0.2% by weight, Emollient oil 0.1% by weight, Inch extract 0.05% by weight, Pine bark extract 0.1% by weight, Pine needle bud extract 0.3% by weight, Panthenol 0.08% by weight, Salicylic acid A cosmetic product was prepared by mixing 0.1% by weight of Acid, 0.15% by weight of Polyquaternium-7, 0.3% by weight of Niacinamide, 0.5% by weight of Jojoba Seed Oil, 1.5% by weight of Behenyl Alcohol, 1.3% by weight of Myristyl Alcohol, 0.5% by weight of Cyclodextrin, 0.05% by weight of Fragrance, and the remaining amount of deionized water.

[0167] 1. Evaluation of improvement in skin brightness The skin brightness improvement effects of the cosmetics produced in Examples 1, 2, and 3, and the cosmetics produced in Comparative Examples 1 and 2, were evaluated.

[0168] Figure 1 is a graph showing the skin brightness improvement effect of the cosmetics produced in Examples 1, 2, and 3, and the cosmetics produced in Comparative Examples 1 and 2.

[0169] The aforementioned effect on improving skin brightness was assessed by measuring the skin brightness of 40 women aged 20-40 using a Chromameter CR-400 before use. Then, the skin brightness was remeasured after individually using the cosmetics according to Examples 1, 2, and 3, and the cosmetics according to Comparative Examples 1 and 2.

[0170] The measurement results are shown in Table 1 and Figure 1 below, and the measured values ​​are shown as the average of three measurements excluding the maximum and minimum values ​​of L*.

[0171] [Table 1]

[0172] Referring to Table 1 and Figure 1, it can be confirmed that the cosmetics produced in Examples 1, 2, and 3 showed a significant improvement in skin brightness compared to the cosmetics produced in Comparative Examples 1 and 2.

[0173] 2. Evaluation of the effect on improving skin wrinkles The skin wrinkle-improving effects of the cosmetics produced in Examples 1, 2, and 3, and the cosmetics produced in Comparative Examples 1 and 2, were evaluated.

[0174] Figure 2 is a graph showing the skin wrinkle improvement effects of the cosmetics produced in Examples 1, 2, and 3, and the cosmetics produced in Comparative Examples 1 and 2.

[0175] The aforementioned effect on improving skin wrinkles was measured in 50 women aged 30 and over.

[0176] The cosmetics according to Examples 1, 2, and 3, and the cosmetics according to Comparative Examples 1 and 2, were applied twice a day (morning and evening) for 10 weeks, mainly around the eyes, and the wrinkle-improving effect on the subjects' skin was measured using the ANTERA3D skin wrinkle improvement measuring device.

[0177] The test results are recorded using the A1 (A1 (10 weeks) to A1 (0 weeks)) values ​​of the measuring instrument. The A1 value indicates the viscoelastic properties of skin wrinkles, and the results are shown in Table 2 and Figure 2 below.

[0178] [Table 2]

[0179] Referring to Table 2 and Figure 2 above, it can be confirmed that the cosmetics produced in Examples 1, 2, and 3 are significantly superior in their effect on improving skin wrinkles compared to the cosmetics produced in Comparative Examples 1 and 2.

[0180] 3. Experiments on improving skin whitening Melanin tyrosinase is the main enzyme that produces melanin pigment. To confirm the whitening effect of the cosmetic according to Example 1, the enzyme activity inhibitory ability of the test substance was measured by comparing the amount of DOPA (dihydroxyphenylalanine) or dopachrome produced from tyrosinase, the main enzyme in melanin synthesis.

[0181] The test method involved using tyrosine as a substrate, adding it to an enzyme reaction solution, allowing it to react for a certain period of time, and then measuring the absorbance at 475 nm, which is the absorption wavelength of the reaction product, dopachrome.

[0182] 500 μl of 0.1 M Potassium phosphate buffer solution (pH 6.8) was mixed with 500 μl of L-tyrosine (0.3 mg / ml) and 450 μl each of the cosmetic compositions from Example 1 and Comparative Example 1. The mixture was reacted on a 37°C water bath for 10 minutes, and then immediately refrigerated for 5 minutes to interrupt the reaction. 50 μl of tyrosinase (1,250 units / ml) was added and incubated for 10 minutes, after which the absorbance was measured at 475 nm. For the control, 450 μl of the sample solvent was added instead of the test solution. For the color correction (Blank), 50 μl of Potassium phosphate buffer solution (pH 6.8, 0.1 M) was added instead of tyrosinase, and the same concentration of sample as the experimental group was added. The tyrosinase inhibitory activity was calculated using the following formula 1 and is shown in Table 3.

[0183] <Number 1> % inhibition = [(AB) / A] × 100

[0184] A: Absorbance after reaction of the reaction solution (control) without the test sample (inhibitor). B: Absorbance of the reaction solution containing the test sample after the reaction.

[0185] [Table 3]

[0186] Referring to Table 3 above, it was confirmed that the cosmetic composition according to Example 1 showed a tyrosinase inhibition rate of 36.87%, while the cosmetic composition according to Comparative Example 1 showed a tyrosinase inhibition rate of 12.33%.

[0187] Furthermore, the cosmetic formulation according to Example 1 showed a slightly lower inhibition rate than the control group, Arbutin. Therefore, it can be said that the cosmetic formulation according to Example 1 is effective in inhibiting tyrosinase.

[0188] 4. Measurement of moisturizing effect Using a capacitance measurement method with a Corneometer CM820 (Courage, Germany), the moisturizing effect was compared and measured using the cosmetic product from Example 1 and the cosmetic product from Comparative Example 1.

[0189] Twenty women aged 20 to 50 years were subjected to a 20-minute exposure to a constant temperature and humidity chamber on the inner surface of their forearms immediately before the start of the experiment, and their skin moisture levels were kept constant. Next, the initial moisture content of each sample application site was measured using a Corneometer C820, and then the cosmetic formulation according to Example 1 and the cosmetic formulation according to Comparative Example 1 were applied.

[0190] The percentage change in skin moisture was measured 20 minutes after application and again 2 hours later. Each measurement site was measured three times, and the average value was used to calculate the percentage change in skin moisture using Equation 2, which is shown in Table 4.

[0191] Moisture content increase / decrease (%) = (Tdi - Tdo) - (Ntdi - NTdo) / ((Ntdi - Ntdo) + Tdo) * 100

[0192] Tdi: nth measurement of the sample application site Tdo: Measurement value before sample application Ntdi: The nth measurement of the area where the sample was not applied. NTdo: Pre-application measurement of the area where the sample was not applied.

[0193] [Table 4]

[0194] Referring to Table 4 above, it can be confirmed that the cosmetic composition according to Example 1 has superior moisturizing effects not only initially but also over time compared to the cosmetic composition according to Comparative Example 1.

[0195] 4. Testing of dosage form stability Stability tests were conducted on the cosmetics manufactured according to Examples 1, 2, and 3 described above.

[0196] The observation period was the same for one week, and stability was observed at various temperatures. After that, the external properties, discoloration, and odor changes were checked, and the results are shown in Table 5.

[0197] [Table 5]

[0198] Referring to Table 5 above, it can be confirmed that the cosmetics produced in Examples 1, 2, and 3 have good dosage form stability.

[0199] Although a preferred embodiment of the present invention has been described above with reference to the attached drawings, a person with ordinary skill in the art to which the present invention belongs can implement the present invention in other specific forms without changing its technical idea or essential features. Therefore, the embodiment described above is illustrative in all respects and not limiting.

Claims

1. A product comprising dried collagen powder, a collagen-containing distilled extract, and a collagen-containing hot water extract, The aforementioned dried collagen powder is a dried collagen powder with a weight-average molecular weight of 200 to 300 daltons (Da). The dried collagen powder is produced by the following steps: preparing at least one collagen selected from the group consisting of fish, collagen extracted from fish scales, chicken foot collagen, cow foot collagen, and pig foot collagen; sterilizing the collagen; mixing 80 to 120 parts by weight of the sterilized collagen, 20 to 40 parts by weight of proteolytic enzyme, and 150 to 250 parts by weight of purified water to produce a collagen enzyme mixture; heating the collagen enzyme mixture in a water bath at a temperature of 57 to 635°C for 1 to 5 hours to produce a collagen enzyme hydrolysate; decolorizing and deodorizing the collagen enzyme hydrolysate by immersing it in sodium hypochlorite for 10 to 60 minutes; concentrating the decolorized and deodorized collagen enzyme hydrolysate to produce a collagen concentrate; and spray-drying the collagen concentrate at a temperature of 160 to 180°C. The collagen-containing distilled extract is produced by preparing at least one collagen selected from the group consisting of fish, collagen extracted from fish scales, chicken foot collagen, cow foot collagen, and pig foot collagen, sterilizing the collagen, adding distilled water in an amount of 10 to 30 times (v / w) to the sterilized collagen, heating the mixture at 60 to 80°C for 10 to 50 minutes, then distilling and extracting while heating at 100°C, recovering the distillate at 30 to 40% of the total weight of the mixture to produce a collagen distilled extract, and filtering the collagen distilled extract through a filter. The collagen-containing hot water extract is produced by preparing at least one collagen selected from the group consisting of fish, collagen extracted from fish scales, chicken foot collagen, cow foot collagen, and pig foot collagen, sterilizing the collagen, mixing it with purified water in a weight ratio of 80 to 120 parts by weight per 100 parts by weight of the total content of the sterilized collagen, heating it at a temperature of 90 to 100°C for 1 to 3 hours to perform hot water extraction, and then filtering the collagen hot water extract through a filter. The dried collagen powder 0.1-0.3% by weight, the collagen-containing distilled extract 0.5-1.5% by weight, the collagen-containing hot water extract 0.1-1% by weight, and caffeic acid 0.1-1% by weight, soybean-derived lysolecithin 0.1-0.5% by weight, herbal fermented extract 0.5-1.5% by weight, coenzyme Q10 Q10) 0.08-0.12% by weight, tocopherol acetate 0.1-0.3% by weight, butylene glycol 0.5-1.5% by weight, stearyltrimethylammonium chloride 0.1-0.3% by weight, L-aspartic acid 0.08-0.12% by weight, sorbitol 0.05-0.15% by weight, stearyl alcohol 0.5-1.5% by weight, cyclopentasiloxane 0.8-1.2% by weight, vitamin C 0.1-0.5% by weight, madecassoside 0.05-0.15% by weight, olive liquid 0.01-0.1% by weight, sodium laureth sulfate 0.8-1.2% by weight, cocamidomypa MIPA 0.1-0.5% by weight, brewer's yeast extract 0.05-0.15% by weight, acai berry extract 0.01-0.1% by weight, emollient oil 0.08-0.12% by weight, inchin extract 0.01-0.1% by weight, sansai extract 0.01-0.05% by weight, grapefruit seed extract 0.01-0.05% by weight, sodium alginate 0.01-0.05% by weight, bacon bark extract 0.01-0.05% by weight, pine needle sprout extract 0.01-0.05% by weight, panthenol 0.04-0.08% by weight, salicylic acid Contains 0.01-0.05% by weight of Acid, 0.08-0.12% by weight of Polyquaternium-7, 0.07-0.13% by weight of Niacinamide, 0.1-0.3% by weight of Jojoba Seed Oil, 0.1-1% by weight of Behenyl Alcohol, 0.5-1.5% by weight of Myristyl Alcohol, 0.07-0.13% by weight of Cyclodextrin, 0.01-0.1% by weight of Fragrance, and the remainder of deionized water. A skin-improving cosmetic composition characterized by the following features.

2. A method for producing the dried collagen powder, the collagen-containing distilled extract, and the collagen-containing hot water extract contained in the skin-improving cosmetic composition according to Claim 1, The dried collagen powder is produced by preparing at least one collagen selected from the group consisting of fish, collagen extracted from fish scales, chicken foot collagen, cow foot collagen, and pig foot collagen, sterilizing the collagen, mixing 80 to 120 parts by weight of the sterilized collagen, 20 to 40 parts by weight of proteolytic enzyme, and 150 to 250 parts by weight of purified water to produce a collagen enzyme mixture, heating the collagen enzyme mixture in a water bath at a temperature of 57 to 635°C for 1 to 5 hours to produce a collagen enzyme hydrolysate, immersing the collagen enzyme hydrolysate in sodium hypochlorite for 10 to 60 minutes to decolorize and deodorize, concentrating the decolorized and deodorized collagen enzyme hydrolysate to produce a collagen concentrate, and spray-drying the collagen concentrate at a temperature of 160 to 180°C. The collagen-containing distilled extract is produced by preparing at least one collagen selected from the group consisting of fish, collagen extracted from fish scales, chicken foot collagen, cow foot collagen, and pig foot collagen, sterilizing the collagen, adding 10 to 30 times (v / w) distilled water to the sterilized collagen, heating the mixture at 60 to 80°C for 10 to 50 minutes, then distilling and extracting while heating at 100°C, recovering the distillate at 30 to 40% of the total weight of the mixture to produce the collagen distilled extract, and filtering the collagen distilled extract through a filter. The collagen-containing hot water extract is produced by preparing at least one collagen selected from the group consisting of fish, collagen extracted from fish scales, chicken foot collagen, cow foot collagen, and pig foot collagen, sterilizing the collagen, mixing it with purified water in a weight ratio of 80 to 120 parts by weight per 100 parts by weight of the total content of the sterilized collagen, heating it at a temperature of 90 to 100°C for 1 to 3 hours to perform hot water extraction, and then filtering the collagen hot water extract. A method characterized by the following:

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