Skin-improving cosmetic composition containing polysaccharides, yeast extract, and fermented product of a bacterial strain having probiotic properties as active ingredients.

Abstract

The present invention relates to a cosmetic composition that contains polysaccharides, yeast extract, and a fermented product of a strain having probiotic properties as active ingredients, and that is safe for the human body while having the effect of improving skin conditions. The cosmetic composition can be used to improve the microbial flora living on the skin, soothe the skin, reduce skin wrinkles, improve skin elasticity, soothe the scalp, improve scalp oiliness, prevent hair loss, or promote hair growth.

Description

[Technical Field]

[0001] The present invention relates to a topical composition for improving skin, comprising polysaccharides, yeast extract, and a fermented product of a bacterial strain having probiotic properties as active ingredients. [Background technology]

[0002] As living standards improve, interest in not only health but also beauty increases daily, and efforts to achieve beautiful skin have become commonplace, with a wide variety of cosmetic compositions available for skincare.

[0003] From a cosmetic standpoint, hair plays a crucial role. Hair is a thin, keratinized structure produced from the surface of the skin. It acts as a cushion against external impacts, protects the body from external stimuli such as direct sunlight, cold, friction, and dangers, and helps to expel harmful heavy metals such as arsenic, mercury, and zinc from the body. Today, its cosmetic aspect as an adornment is also emphasized.

[0004] It is known that various factors, including changes in diet and increased internal and external stress, can trigger deterioration of hair condition, including hair loss. However, the scalp and hair are generally interdependent, and the condition of the scalp is known to be one of the biggest factors influencing hair (Int.J.Trichology., 2018 Nov-Dec;10(6):262-270).

[0005] On the other hand, topical formulations for the skin, including scalp and hair care, are available in various forms in terms of raw materials, form, and function. However, most of these contain artificial compounds to kill harmful bacteria on the skin or to adjust oil content. While these artificial compounds offer excellent functional advantages, they can sometimes irritate the skin and, in the long term, may actually cause skin damage.

[0006] Therefore, there is a need to improve upon the shortcomings of such conventional topical formulations for skin application and to develop compositions that are safe for the human body while effectively improving skin condition. [Prior art documents] [Non-patent literature]

[0007] [Non-Patent Document 1] Int.J.Trichology., 2018 Nov-Dec;10(6):262-270 [Overview of the project] [Problems that the invention aims to solve]

[0008] The present invention was created to solve the problems of the prior art described above, and the object of the present invention is to provide a skin-improving cosmetic composition that contains plant-derived polysaccharides, yeast-derived extracts, and fermented products of bacterial strains having probiotic properties as active ingredients, and that can exhibit the effect of increasing beneficial bacteria on the skin or reducing harmful bacteria. [Means for solving the problem]

[0009] The present invention provides a cosmetic composition for improving skin, which contains polysaccharides, yeast extract as a microbial-derived extract, and a fermented product of a microbial strain having probiotic properties as active ingredients.

[0010] As used in this invention, the term "probiotics" refers to microorganisms that exist in the human body and can have beneficial effects on human health, and the effects described above, encompassing all metabolites such as dead cells, extracts, and fermented products of the above microorganisms. "Prebiotics" refers to feed that can be used to promote the growth, development, and activity of the above microorganisms.

[0011] The term "microbiota" as used in this invention refers to a group of microbial cells that normally exist in specific parts of the human body and can influence human health.

[0012] In this invention, while fermented products of bacterial strains having probiotic properties did not on their own show meaningful effects in increasing beneficial bacteria or reducing harmful bacteria to improve skin condition, it was confirmed that a composition further containing polysaccharides as a prebiotic and a yeast-derived extract as another probiotic, along with the above-mentioned fermented bacterial strain, can exhibit prebiotic properties that promote the growth and proliferation of beneficial bacteria, and this composition is provided as a composition for improving scalp or skin condition.

[0013] The polysaccharide in this invention may be one or more selected from the group consisting of inulin, beta-glucan, and maltodextrin. Such polysaccharides contribute to enhancing immunity by improving the activity of immune cells in the human body and have the effect of reducing oxidative stress in cells.

[0014] Inulin is a polysaccharide used as a means of energy storage in many plants, including chicory, wheat, onions, bananas, garlic, and asparagus, and is generally obtained from the roots or rhizomes of these plants. When applied to the skin, inulin can protect probiotic microorganisms present in the skin from antimicrobial substances. Inulin is represented by the following chemical formula 1 (C 6n H 10n+2 O 5n+1 ) contains a glucosyl moiety at the end of the chain, with repeating fructosyl moieties in between. [ka]

[0015] The above value of n may be between 2 and 60, but is not limited thereto. The inulin used in this invention is obtained from chicory roots, but it may also be purchased and used from commercially available sources in Japan or overseas.

[0016] Beta-glucan is a polysaccharide formed in the cell walls of grains, bacteria, and fungi. When beta-glucan is applied to the skin, it has the effect of increasing antioxidant activity and anti-wrinkle activity in skin cells, improving UV blocking, wound healing, and moisturizing power. The beta-glucan represented by Chemical Formula 2 below contains repeating glucosyl moieties glycosidically linked at the beta 1,3 positions.

Chem.

[0017] The above n may be 2 to 100, but is not limited thereto. The beta-glucan in the present invention can be obtained by extraction from mushroom fruiting bodies, but commercially available products at home and abroad may also be purchased and used.

[0018] Maltodextrin is a polysaccharide formed by partial hydrolysis of plant starch. When maltodextrin is applied to the skin, it increases antioxidant activity in skin cells. The maltodextrin represented by Chemical Formula 3 below contains repeating glucosyl moieties glycosidically linked at the alpha 1,4 positions.

Chem.

[0019] The above n may be 2 to 20, but is not limited thereto. In the present invention, it can be obtained by hydrolyzing corn starch or wheat starch, but commercially available products at home and abroad may also be purchased and used.

[0020] In the present invention, the term "yeast extract" can encompass extracts obtained by extracting yeast with a solvent, fractions obtained by hydrolyzing yeast cells, yeast culture media in which yeast and culture material coexist, extracts obtained from the above-mentioned fraction or culture media, filters obtained by filtering yeast from the above-mentioned fraction or culture media, and dilutions and dried products of the above-mentioned fraction or extract. For example, the yeast extract may be a fraction obtained by extracting yeast with a pharmaceutically acceptable organic solvent, or by hydrolyzing yeast cells using an enzyme and then separating it with a solvent.

[0021] The yeast extract used in this invention may be one or more selected from the group consisting of beer-derived yeast extract and truffle-derived yeast extract.

[0022] The beer-derived yeast extract may be obtained by culturing yeast of the genus Saccharomyces, which belongs to the Ascomycetes, or by purchasing and culturing the same species of yeast, then extracting the culture after sterilization. Preferably, the yeast is beer yeast (Saccharomyces cerevisiae). Beer yeast is also called baker's yeast and is used in the production of alcoholic beverages, including beer, and bread. The beer-derived yeast extract can be obtained by drying the yeast that is separated after fermenting the wort and filtering the beer during the beer production process using beer yeast. The beer-derived yeast extract is non-fermentable and contains large amounts of carbohydrates, proteins, nucleic acids, vitamin B, and minerals such as phosphorus. Vitamin B group has the effect of improving skin health and hair condition. In this invention, it can be obtained by isolating and culturing yeast used in the beer production process and then sterilizing it, but commercially available products from Japan or overseas may also be purchased and used.

[0023] The truffle-derived yeast extract may be obtained by isolating and culturing yeast from the microorganisms present in truffles, or by purchasing and culturing the same species of yeast found to be present in truffles, and then extracting the culture after sterilization. Truffle-derived yeast extract contains a large amount of beta-glucan and a small amount of B vitamins. In this invention, the extract can be obtained by the method exemplified above, but commercially available extracts from Japan or overseas may also be purchased and used.

[0024] In the present invention, the bacterial strain having probiotic properties may be one or more anaerobic bacterial strains selected from the group consisting of Lactobacillus strains and Bifidobacterium strains. Therefore, the fermentation product of the bacterial strain having probiotic properties may encompass, but is not limited to, the fermentation metabolites of the above-mentioned bacterial strain.

[0025] In the present invention, the fermented product is not only the fermented substance obtained from the bacterial strain, but also the culture medium of the bacterial strain in which the bacterial strain and culture material coexist, the fermented product obtained by filtering the bacterial strain from the culture medium, the fermented product obtained by sterilizing the bacterial strain from the culture medium and filtering it, the extract obtained by extracting the above-mentioned fermented product or culture medium containing it, and the diluted or dried fermented product or extract of the above-mentioned fermented product or extract. things It can encompass and mean things like that. Preferably, fermented products can mean fermented lysates and fermented filtrates.

[0026] For example, the term "fermented product" can encompass both fermentation lysate and fermentation filtrate. Fermentation lysate refers to the substance obtained after fermentation by an anaerobic bacterial strain, by using conditions such as heat, pH, enzymes, and pressure to kill the bacteria. Fermentation lysate contains not only fermentation metabolites but also various useful components within the bacterial strain. Fermentation filtrate refers to the supernatant obtained after fermentation by an anaerobic bacterial strain, by removing the microorganisms containing the strain through separation methods such as filtration.

[0027] Lactobacillus strains are Gram-positive bacteria distributed throughout the natural environment, including inside the human body, plants, and soil. Lactobacillus strains can produce lactic acid through fermentation metabolism of hexoses. As a result of metabolism within the human body, Lactobacillus strains produce lactic acid and hydrogen peroxide, which can suppress the growth of harmful bacteria, including certain pathogens.

[0028] In this invention, the Lactobacillus strains can encompass isomorphic lactic acid fermentation strains that ferment one hexose molecule into two lactic acid molecules, and heteromorphic lactic acid fermentation strains that further produce not only lactic acid molecules but also ethanol, acetic acid, carbon dioxide, etc., from hexose molecules. For example, Lactobacillus strains include Lactobacillus acidophilus, Lactobacillus delbrueckii, Lactobacillus helveticus, Lactobacillus salivarius, Lactobacillus casei, Lactobacillus plantarum, Lactobacillus sakei, and Lactobacillus brevis. Lactobacillus strains may include, but are not limited to, L. brevis, L. buchneri, L. fermentum, L. reuteri, L. rhamnosus, L. paracasei, L. Johnsonii, L. bulgaricus, etc. Preferably, the Lactobacillus strains may include at least one of Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus plantarum, Lactobacillus fermentum, Lactobacillus paracasei, and Lactobacillus bulgaricus. More preferably, the Lactobacillus strain may be at least one of Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus fermentum, and Lactobacillus paracasei. Most preferably, the Lactobacillus strain may be at least one of Lactobacillus acidophilus and Lactobacillus plantarum.

[0029] Therefore, the fermented product of the Lactobacillus strain in the present invention may contain fermentation metabolites of the strain with respect to hexoses, and preferably, it may be a fermentation lysate in which the strain has been killed.

[0030] Bifidobacterium strains are Gram-positive bacteria distributed throughout the natural environment, including human skin, food, and soil. Bifidobacterium strains are heteromorphic lactic acid fermenting bacteria that produce lactic acid, acetic acid, formic acid, and other substances through a unique hexose fermentation metabolism utilizing fructose-6-phosphate phosphoketolase.

[0031] The Bifidobacterium strains in the present invention may include, but are not limited to, Bifidobacterium animalis, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium dentium, Bifidobacterium longum, and Bifidobacterium pseudolongum. Preferably, the Bifidobacterium strain may be at least one of Bifidobacterium animalis, Bifidobacterium bifidum, and Bifidobacterium longum.

[0032] The fermented product of the Bifidobacterium strain in the present invention may contain fermentation metabolites of the strain with respect to hexoses, and preferably may be a fermentation lysate in which the strain has been killed.

[0033] A cosmetic composition for skin improvement according to one embodiment of the present invention may contain inulin, beta-glucan, maltodextrin, brewer's yeast extract, truffle-derived yeast extract, Lactobacillus ferment lysate, or Bifidobacterium ferment lysate as active ingredients.

[0034] Preferably, the cosmetic composition may contain inulin, beta-glucan, maltodextrin, brewer's yeast extract, truffle-derived yeast extract, Lactobacillus ferment lysate, and Bifidobacterium ferment lysate in equal proportions. For example, the cosmetic composition may contain inulin, beta-glucan, maltodextrin, brewer's yeast extract, truffle-derived yeast extract, Lactobacillus ferment lysate, and Bifidobacterium ferment lysate in the weight ratio a:b:c:d:e:f:g in that order. Here, a to g may each be 1 to 10, preferably a to g each be 1 to 5, and most preferably a to g can all be 1.

[0035] A skin-improving cosmetic composition according to one embodiment of the present invention may contain, based on 100% by weight of the total, one or more active ingredients selected from the group consisting of inulin, beta-glucan, maltodextrin, brewer's yeast extract, truffle yeast extract, Lactobacillus ferment lysate, and Bifidobacterium ferment lysate in an amount of 0.00001 to 10% by weight. Preferably, it can contain 0.0001 to 1% by weight, more preferably 0.0005 to 0.5% by weight, and most preferably 0.001 to 0.1% by weight.

[0036] As used in this invention, the term "skin improvement" can mean not only an improvement in skin condition as observed with the naked eye, but also factors that directly and indirectly affect skin health.

[0037] Specifically, the skin-improving cosmetic composition according to the present invention may be used to improve the microbial flora inhabiting the skin. Improving the microbial flora can include promoting the growth of beneficial bacteria present on the skin to increase their number, or suppressing the growth of harmful bacteria to reduce their number.

[0038] Specifically, the cosmetic composition for skin improvement according to the present invention may be for any one of the following purposes: skin soothing, wrinkle reduction, and skin elasticity improvement. Skin soothing can include preventing or suppressing inflammation in the skin and can also encompass preventing or normalizing skin disorders. Wrinkle reduction or elasticity improvement can also encompass preventing or suppressing damage or volume reduction of the dermis caused by natural factors such as aging and external environmental factors such as ultraviolet radiation and oxidative stress.

[0039] Specifically, the cosmetic composition for improving skin according to the present invention may be for application to the scalp. For example, the cosmetic composition for improving scalp may be for any one of the following purposes: for soothing the scalp, for improving scalp oiliness, for preventing hair loss, and for improving hair growth. Soothing the skin may mean preventing or suppressing inflammation in the scalp. Improving scalp oiliness may mean reducing the amount of oil secreted when excess oil is secreted from the skin. Preventing hair loss or improving hair growth may include increasing the activity of hair papilla cells in the scalp, suppressing inflammatory factors against cells that make up the skin, or increasing the rate of collagen biosynthesis in cells that make up the skin.

[0040] Preferably, "improvement of skin" can mean an increase in beneficial bacteria and a decrease in harmful bacteria present on the skin. The above-mentioned beneficial and harmful bacteria are classified based on the effect of the relevant bacterial strain on human skin health, and include, for example, strains of the genus Staphylococcus, but are not limited to these.

[0041] For example, some strains of the Staphylococcus genus, such as Staphylococcus epidermidis, also known as Staphylococcus epidermidis, do not act as pathogens against normal skin. Instead, they produce antimicrobial peptides against other pathogens and have the effect of inhibiting the progression of some cancers, and can therefore be classified as beneficial bacteria.

[0042] For example, bacteria such as Staphylococcus aureus, a strain of the Staphylococcus genus, can be found on the skin surface and in hair follicles, inducing a variety of skin infections and potentially causing disease even in healthy skin, and can therefore be classified as harmful bacteria. If the skin is the scalp, harmful bacteria can include Aspergillus niger, which produces mycotoxins that can induce inflammation of the scalp.

[0043] As described later, a composition according to one embodiment of the present invention can provide a skin-improving effect by improving the microbiome in the human body, such as by increasing beneficial bacteria.

[0044] Human dermal papilla cells are mesenchymal cells present in hair follicles of the scalp and play a crucial role in controlling hair production and the hair growth cycle. Excessive oil on the scalp can induce dermatitis, and inflammatory factors can trigger inflammatory responses in cells present in the scalp. Collagen refers to the connective tissue protein present in the extracellular matrix, and a decrease in collagen biosynthesis in the scalp can lead to weakening of the dermis of the scalp and subsequent shrinkage of hair follicles.

[0045] As described later, a composition according to one embodiment of the present invention can provide skin improvement effects by increasing the activity of specific cells, reducing skin oiliness, suppressing the production of inflammatory factors in skin cells, and increasing the collagen biosynthesis rate.

[0046] As used in this invention, the term "cosmetic composition" means a composition intended for administration by direct application or spraying onto the skin. As used in this invention, the term "administration" means introducing the composition according to the present invention into a target tissue using conventional methods known in the art to which this invention belongs.

[0047] The skin-improving cosmetic composition according to the present invention may be in the form of a lotion, face lotion, body lotion, nourishing cream, moisturizing cream, eye cream, essence, cosmetic ointment, spray, gel, pack, sunscreen, makeup base, foundation, powder, cleansing cream, cleansing lotion, cleansing oil, cleansing foam, soap, or body wash.

[0048] In the skin-improving cosmetic composition according to the present invention, if the skin is the scalp or hair, the composition may be in the form of a hair tonic, hair conditioner, hair essence, hair lotion, hair nourishing lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nourishing cream, hair moisturizing cream, hair massage cream, hair wax, hair aerosol, hair pack, hair nourishing pack, hair soap, hair cleansing foam, hair oil, hair drying agent, hair preservation treatment agent, hair dye, hair waving agent, hair bleaching agent, hair gel, hair glaze, hair dresser, hair lacquer, hair moisturizer, hair mousse, or hair spray.

[0049] When the composition of the present invention is put into a liquid dosage form, the composition of the present invention may contain a carrier for the above-mentioned active ingredient. The carrier may be, but is not limited to, water, ethanol, castor oil, glycerin, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, or fatty acid esters such as sorbitan. These may be used alone or in combination of two or more.

[0050] When the composition of the present invention is formulated into a paste, cream, or gel, the composition may include a carrier for the active ingredient. The carrier may be, but is not limited to, animal oils, vegetable oils, waxes, paraffin, starch, tracant, cellulose derivatives such as hydroxyethyl, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, cetostearyl alcohol, or stearyltriethylammonium chloride. These may be used alone or in combination of two or more.

[0051] When the composition of the present invention is formulated as a powder or spray, the composition may include a carrier for the active ingredient. The carrier may be, but is not limited to, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder. These may be used alone or in combination of two or more. When the composition of the present invention is formulated as a spray, it may further include a propellant such as chlorofluorohydrocarbon, propane, butane, or dimethyl ether.

[0052] When the composition of the present invention is formulated into a soap, the composition may include a carrier for the active ingredient. The carrier may be, but is not limited to, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolysates, isethionates, lanolin derivatives, aliphatic alcohols, vegetable oils, glycerol, sugars, etc. These may be used alone or in combination of two or more.

[0053] The composition of the present invention may further contain additives commonly used in cosmetic compositions. These additives may, but are not limited to, purified water, surfactants, humectants, lower alcohols, chelating agents, disinfectants, antioxidants, preservatives, pigments, and fragrances. These may be used individually or in combination of two or more.

[0054] The present invention provides applications for one or more polysaccharides, yeast extracts, and fermented products of microbial strains having probiotic properties. More specifically, the present invention provides applications for one or more polysaccharides, yeast extracts, and fermented products of microbial strains having probiotic properties in cosmetic compositions for improving skin. More specifically, the present invention provides applications in methods for producing one or more cosmetic compositions for improving skin, which are polysaccharides, yeast extracts, and fermented products of microbial strains having probiotic properties.

[0055] Furthermore, the present invention provides applications for one or more polysaccharides, yeast extracts, and fermented strains having probiotic properties in methods for improving skin, improving the microbial flora residing on the skin, soothing the skin, improving skin wrinkles, improving skin elasticity, soothing the scalp, improving scalp oiliness, preventing hair loss, or improving hair growth.

[0056] Furthermore, the present invention provides a method for improving skin by treating a target body with a cosmetic containing one or more of polysaccharides, yeast extracts, and fermented strains having probiotic properties; a method for improving the microbial flora inhabiting the skin; a method for soothing the skin; a method for improving skin wrinkles; a method for improving skin elasticity; a method for soothing the scalp; a method for improving scalp oiliness; a method for preventing hair loss; or a method for improving hair growth.

[0057] As described above, all components included in this invention do not exceed the limits set by regulations in each country. Preferably, the cosmetic composition of this invention may contain the above-mentioned individual components within a range that does not exceed the maximum usage amount stipulated in the "Cosmetic Safety and Technical Specifications" established by the Chinese government. [Effects of the Invention]

[0058] The cosmetic composition for skin improvement according to the present invention contains polysaccharides as prebiotics, yeast-derived extracts and fermented bacterial strains as probiotics, and achieves superior effects on the proliferation of beneficial bacteria and the suppression of harmful bacteria on the skin compared to the application of prebiotics or probiotics alone. The cosmetic composition is harmless to the human body and has the effect of improving the condition of the skin by improving the skin's microbiome.

[0059] Specifically, the cosmetic composition for improving skin according to the present invention has the effect of increasing the activity of hair papilla cells and improving scalp health and hair condition.

[0060] Furthermore, the cosmetic composition for improving skin according to the present invention has the effect of improving skin condition by suppressing the production of inflammatory factors in skin cells.

[0061] Furthermore, the cosmetic composition for improving skin according to the present invention has the effect of improving the condition and health of the skin by increasing the rate of collagen biosynthesis in skin cells.

[0062] Furthermore, the skin-improving cosmetic composition according to the present invention has the effect of removing foreign substances and oils without causing side effects on the skin, thereby improving the oil-moisture balance on the skin surface. [Brief explanation of the drawing]

[0063] [Figure 1] These are photographs of the scalp (a) before application and (b) after one application of the hair shampoo according to an embodiment of the present invention. [Figure 2] These are photographs of the scalp (a) before application and (b) after one application of the hair shampoo according to an embodiment of the present invention. [Figure 3] These are photographs of the scalp (a) before application and (b) after one application of the hair shampoo according to an embodiment of the present invention. [Modes for carrying out the invention]

[0064] The object, particular advantages, and novel features of the present invention will become clearer from the following detailed description and preferred embodiments. However, these embodiments are for illustrative purposes only, and the scope of the invention is not limited to these embodiments. In describing the present invention, if a specific description of related prior art is deemed to unnecessarily obscure the gist of the invention, such detailed description will be omitted.

[0065] Manufacturing example: A complex containing polysaccharides, yeast extract, and a fermented product of a bacterial strain with probiotic properties.

[0066] In the following examples, commercially available products were used for inulin (Beneo), beta-glucan (QUEGEN BIOTECH Co., LTD.), maltodextrin (SAMYANG), and brewer's yeast extract (ACTIVON). For the truffle-derived yeast extract, the extract was obtained by isolating yeast from truffles, culturing it at 37°C for 48 hours, and then sterilizing it. For the Lactobacillus fermentation lysate and Bifidobacterium fermentation lysate, the extracts were obtained by culturing Lactobacillus and Bifidobacterium (Chr. Hansen) at 37°C for 48 hours and then sterilizing them.

[0067] (Production Example 1) A compound was produced by mixing 10g each of inulin, brewer's yeast extract, and Lactobacillus fermentation lysate. (Production Example 2) A compound was produced by mixing 10g each of beta-glucan, brewer's yeast extract, and Lactobacillus fermentation lysate. (Production Example 3) A compound was produced by mixing 10g each of maltodextrin, brewer's yeast extract, and Lactobacillus fermentation lysate. (Production Example 4) A compound was produced by mixing 10g each of inulin, truffle-derived yeast extract, and Bifidobacterium fermentation lysate. (Production Example 5) A compound was produced by mixing 10g each of beta-glucan, truffle-derived yeast extract, and Bifidobacterium fermentation lysate. (Production Example 6) A compound was produced by mixing 10g each of maltodextrin, truffle-derived yeast extract, and Bifidobacterium fermentation lysate. As (production example 7), a compound was prepared by mixing 10g each of inulin, beta-glucan, maltodextrin, brewer's yeast extract, truffle yeast extract, Lactobacillus fermentation lysate, and Bifidobacterium fermentation lysate.

[0068] Examples. Cosmetic compositions containing polysaccharides, yeast extracts, and fermented products of bacterial strains having probiotic properties as active ingredients.

[0069] Example 1-1. Hair shampoo composition

[0070] A hair shampoo composition having the composition shown in Table 1 below was manufactured.

[0071] [Table 1]

[0072] Examples 1-2. Hair shampoo composition

[0073] A hair shampoo composition having the composition shown in Table 2 below was manufactured.

[0074] [Table 2]

[0075] Example 2. Hair Treatment Composition

[0076] A hair treatment composition having the composition shown in Table 3 below was manufactured.

[0077] [Table 3]

[0078] Example 3. Hair Tonic

[0079] A hair tonic composition having the composition shown in Table 4 below was prepared.

[0080] [Table 4]

[0081] Example 4. Body wash

[0082] A body wash composition having the composition shown in Table 5 below was manufactured.

[0083] [Table 5]

[0084] Example 5. Body lotion

[0085] A body lotion composition having the composition shown in Table 6 below was manufactured.

[0086] [Table 6]

[0087] Experimental Example 1: Confirmation of the effect on the growth of beneficial bacteria.

[0088] To confirm the effect of the compound in the production example on the growth of beneficial bacteria, Staphylococcus epidermidis was selected as the beneficial bacterium and Staphylococcus aureus as the harmful bacterium. The bacteria were stored at -80°C before use, and 500 μl of stock of each bacterium was inoculated into 100 mL of tryptic soy broth (TSB) medium in each medium. Then, the cultures were rotated at 210 rpm for 17 hours at 37°C, after which 4 mL of the culture solution was taken and inoculated into 40 mL of TSB, and subcultured at 210 rpm for 8 hours at 37°C.

[0089] After 8 hours, the optical density (OD) value was adjusted to 0.65, and the composites prepared according to Production Examples 1-7 above were inoculated at a 1% concentration. The initial optical density was then measured. Subsequently, the cultures were rotated at 210 rpm for 16 hours at 37°C, and the optical density was measured again. For each medium, the difference between the optical density measurement after 16 hours and the initial measurement was standardized and evaluated by separating the initial measurement.

[0090] For comparative examples, samples were inoculated with 1% concentrations of inulin, beta-glucan, maltodextrin, brewer's yeast extract, truffle yeast extract, Lactobacillus fermentation lysate, and Bifidobacterium fermentation lysate. The control group consisted of an untreated group.

[0091] The inoculation results of the above manufacturing example and comparative example were compared with the untreated group, and the average increase or decrease in beneficial and harmful bacteria was converted to a percentage. The results are shown in Table 7 below.

[0092] [Table 7]

[0093] As shown in Table 7, despite being polysaccharides with prebiotic properties, inulin, beta-glucan, and maltodextrin alone do not show a meaningful effect on increasing beneficial bacteria and reducing harmful bacteria. On the contrary, fermentation lysates with probiotic properties alone actually decrease beneficial bacteria and increase the growth of harmful bacteria.

[0094] In contrast, the composites produced by Production Examples 1 to 7 of the present invention were shown to be remarkably superior in increasing beneficial bacteria and reducing harmful bacteria compared to comparative examples using polysaccharides, yeast extracts, or fermentation lysates alone. This confirmed that there is a synergistic effect on increasing beneficial bacteria and reducing harmful bacteria when polysaccharides, which are prebiotics, are used in combination with yeast extracts and fermentation lysates, which are probiotics. In particular, Production Example 7 was confirmed to show the most superior effect on increasing beneficial bacteria and reducing harmful bacteria compared to the other production examples.

[0095] Experimental Example 2: Confirmation of hair papilla cell activity

[0096] To confirm the effect of the compound used in the manufacturing example on dermal papilla cells, 4-8 passage human dermal papilla cells (Promocell, C-12071) were used as the cell line, and follicle dermal papilla cell growth media (Promocell, C-26501) was used as the initial culture medium.

[0097] Cells were seeded in 96-well plates at a level of 3,000 to 6,000 cells per well and cultured for 24 hours in an incubator at 37°C with a carbon dioxide concentration of 5%. Then, the culture medium was replaced with DMEM medium supplemented with 0.1% FBS (fetal bovine serum) and cultured for another 24 hours at 37°C with a carbon dioxide concentration of 5%. Subsequently, the culture medium was replaced with DMEM medium supplemented with 0.1% FBS, which had been treated with the composites described in Production Examples 1 to 7 at different concentrations, and cultured for 48 hours at 37°C with a carbon dioxide concentration of 5%. 10 μl of CCK-8 solution was treated in each well and cultured at 37°C for 1 hour, after which the optical density at 450 nm was measured.

[0098] For each well, the difference between the optical density measurement after 48 hours and the initial measurement was evaluated by standardizing the initial measurement, and the mean values ​​are shown in Table 8 below. The control group was treated with 1 μM minoxidil. Statistical significance was determined by assuming equal variances and deriving the p-value (significant difference p<0.05).

[0099] [Table 8]

[0100] As shown in Table 8, the composites produced by Production Examples 1 to 7 of the present invention were confirmed to have a synergistic effect on the activity of dermal papilla cells. In particular, Production Example 7 was confirmed to show the best effect in increasing the activity of dermal papilla cells compared to the other production examples.

[0101] Experimental Example 3. Confirmation of Suppression of Inflammatory Factors

[0102] To confirm the effect of the compound in the manufacturing example on NO production as an inflammatory factor in cells, mouse Raw264.7 cell line was used, and the initial culture medium was Complete RPMI (RPMI:FBS:antibiotic = 10:1:0.1) with NaOH added to adjust the final pH to 8.5.

[0103] Raw264.7 cells were cultured at 37°C with a carbon dioxide concentration of 5% for 24 hours. When the cells began to float on the culture medium, they were seeded into a 24-well plate prepared with DMEM culture medium and cultured at 37°C with a carbon dioxide concentration of 5% for 24 hours. Then, the medium was replaced with serum-free DMEM culture medium, and the complexes prepared according to Production Examples 1-7 were diluted to the following concentrations and treated with LPS at 1 μg / mL. After culturing at 37°C with a carbon dioxide concentration of 5% for 24 hours, the supernatant was mixed with Griess reagent (Sigma-Aldrich) in a 1:1 ratio, and the NO production inhibitory ability was evaluated by absorbance. The average values ​​are shown in Table 9 below.

[0104] NO generation suppression ability (%) = {1 - (amount of NO generated when compound is added / amount of NO generated in the untreated group)} × 100

[0105] The control group was L-NMMA1(N), an NO inhibitor. G (Methyl-L-arginine acetate salt) 20 μg / mL was used.

[0106] [Table 9]

[0107] As shown in Table 9, the composites produced by Production Examples 1-7 of the present invention were confirmed to have the ability to suppress NO production as an inflammatory factor in cells. In particular, Production Example 7 was confirmed to have the best NO production suppression ability compared to the other production examples.

[0108] Experimental Example 4. Confirmation of Collagen Biosynthesis Rate

[0109] The effect of the compound from the production example on collagen biosynthesis in human dermal fibroblasts was investigated. Dermal fibroblasts were cultured in Complete DMEM culture medium at 37°C and 5% carbon dioxide for 72 hours. Then, they were seeded in 24-well plates and cultured at 37°C and 5% carbon dioxide for 24 hours. After treatment with the compound from the production example and cultured at 37°C and 5% carbon dioxide for 48 hours, only the supernatant was separated, and the collagen synthesis effect was confirmed by absorbance using the procollagen type I C-peptide (PIP) EIA kit. The increase in collagen biosynthesis was evaluated as follows, and the average values ​​are shown in Table 10 below:

[0110] Collagen biosynthesis increase rate (%) = {(Absorbance of the composite treatment group - Absorbance of the untreated group) / Absorbance of the untreated group} × 100

[0111] The control group used the cytokine TGF-β at a concentration of 10 ng / mL.

[0112] [Table 10]

[0113] As shown in Table 10, it was confirmed that the composites produced by Production Examples 1 to 7 of the present invention have the effect of increasing collagen biosynthesis in fibroblasts. In particular, Production Example 7 was confirmed to show the most superior effect in increasing collagen biosynthesis in fibroblasts compared to the other production examples.

[0114] Experiment Example 5. Confirmation of whether or not scalp oiliness has improved.

[0115] The effect of the hair shampoo composition according to Example 1-1 on improving scalp oiliness was confirmed. Twenty-two adult women aged 20-50 years were included as subjects, and their scalps were selected as the test site. During the experiment, the subjects were prohibited from using other shampoos, conditioners, hair essences, or other products, as well as from undergoing hair treatments such as dyeing or perming.

[0116] The subjects were allowed to rest for 30 minutes in a constant temperature and humidity chamber at 22±2℃ and 50±5% humidity before the experiments were conducted. All experiments were performed in the above constant temperature and humidity chamber. After thoroughly wetting the subjects' hair and scalp with lukewarm water, the subjects applied the same amount of the hair shampoo composition according to Example 1-1 evenly to their hair and scalp, massaged it in, and then rinsed thoroughly with running water.

[0117] Scalp oil content before and after using hair shampoo was evaluated using a Sebumeter (SKIN-O-MAT, Cosmomed GmbH) and a video microscope (Kong PC Camera, Bomtech). A probe cassette with oil-absorbing tape attached was placed on the top of each subject's head and contacted for 30 seconds under the same pressure to allow sufficient oil absorption. After that, the cassette was inserted into the Sebumeter body and the amount of sebum was measured. In addition, the top of each subject's head was observed at 300x magnification using a video microscope under the same lighting conditions.

[0118] The following statistical processing in this experiment was analyzed using SPSS 17.0 for Windows. Table 11 below shows the sebum amount (μg / cm 2 ) before and after one use of the composition of Example 1-1 (The maximum sebum amount measurable by Sebumeter is 350 μg / cm 2 ).

[0119]

Table 11

[0120] Table 12 below shows the analysis results of the paired t-test for analyzing the sebum amount improvement rate (%) and the presence or absence of significant changes after one use of the composition of Example 1-1 ( * p <.05, ** p <.01, *** p <.001): Improvement rate (%) = {(Measured value after one use - Measured value before use) / Measured value before use} × 100

[0121]

Table 12

[0122] In addition, the scalp conditions of individual subjects and the changes in the scalp conditions before and after one use of the composition of Example 1-1 were surveyed, and the results are shown in Tables 13 and 14 below respectively (N = total frequency = number of subjects = 22).

[0123]

Table 13

[0124]

Table 14

[0125] Furthermore, the presence or absence of abnormal reactions to the scalp and hair during the use of the composition of the example was surveyed, and the results are shown in Table 15 below (0: none, 1: weak, 2: medium, 3: severe).

[0126] [Table 15]

[0127] As shown in Tables 11 and 12, after one application of the hair shampoo according to Example 1-1 of the present invention, the amount of sebum improved by more than 80% compared to before, confirming its excellent effect in improving scalp oiliness. These results were consistent with the results of the questionnaires to subjects shown in Tables 13-15, in which more than 90% of subjects reported that the shampoo had a cleansing and sebum-removing effect without side effects.

[0128] Figures 1-3 are photographs showing magnified observation results of three subjects before (a) application of the hair shampoo according to Example 1-1 of the present invention and (b) after one application, confirming that it has excellent cleansing and sebum removal effects.

[0129] Experiment Example 6. Confirmation of whether or not there is improvement in the scalp microbiome.

[0130] The effectiveness of the hair shampoo composition according to Example 1, the hair treatment composition according to Example 2, and the hair tonic composition according to Example 3 in improving the scalp microbiome was confirmed. Twenty-four adult women with an average age of 45 were used as subjects, and their scalps were selected as the test site. During the experimental period, the subjects were prohibited from using other shampoos, conditioners, hair essences, or other products, as well as from hair treatments such as dyeing or perming.

[0131] The subjects were allowed to rest for 30 minutes in a constant temperature and humidity chamber at 20-25°C and 40-60% humidity before the experiments were conducted. All experiments were performed in the aforementioned constant temperature and humidity chamber. After thoroughly wetting their hair and scalp with lukewarm water, the subjects applied equal amounts of the hair shampoo compositions according to Examples 1-1 and 1-2 and the hair treatment composition according to Example 2 to their hair and scalp once a day, massaged it in, and then rinsed thoroughly with running water. In addition, the subjects applied equal amounts of the hair tonic composition according to Example 3 to their scalp once a day, massaged it in, and then allowed it to dry without rinsing.

[0132] For one day after the experimental period, the subjects were kept still in the constant temperature and humidity chamber described above for 30 minutes without adding shampoo or water to their hair or scalp. Then, with the parting at the top of the head fixed in place, a cotton swab was swapped up and down 40 times to collect microorganisms from the scalp, which were then compared and analyzed with microorganisms collected before the experiment.

[0133] The following statistical analysis in this experiment was performed using SPSS. An improvement effect was determined when the significance probability p < 0.05 was found within a 95% confidence interval. For statistical analysis, the paired samples t-test was used when using parametric methods, and the Wilcoxon signed-rank test was used when using non-parametric methods. Table 16 shows the results of analysis of variance (ANOVA) at the genus level before and after the use of the compositions in the examples.

[0134] [Table 16]

[0135] Two weeks after using the compositions according to the examples, statistically significant changes were observed in the fungal flora of nine genera present on the scalp. As shown in Table 16, from one week after using the compositions according to Examples 1-3 of the present invention, the Saccharomyces genus, which is known to contribute to scalp moisturizing and elasticity improvement, increased statistically significantly. Aspergillus, Fusarium, and Penicillum genera, which are known to induce mycosis or dermatitis, decreased statistically significantly after two weeks of use, confirming a statistically significant improvement in the fungal flora on the scalp.

[0136] Experiment Example 7. Confirmation of whether or not there is improvement in the skin microbiome.

[0137] The effectiveness of the body wash composition prepared in Example 4 and the body lotion composition prepared in Example 5 in improving the skin microbiome was confirmed. Twenty-two adult women aged 20-60 years participated as subjects, and the skin on their forearms was selected as the test site. Subjects were prohibited from using any other body products during the experiment.

[0138] The subjects remained at rest for 30 minutes in a constant temperature and humidity chamber at 20-25°C and 40-60% humidity, without washing the test site for more than 8 hours, before the experiments were conducted. All experiments were performed in the above constant temperature and humidity chamber. After wetting the skin of the forearm with lukewarm water, the subjects washed with the body wash composition according to Example 4, and then applied the body lotion composition according to Example 5 evenly.

[0139] After the experimental period, microorganisms were collected from the skin of the forearm by swapping a cotton swab up and down 40 times, and compared and analyzed with microorganisms collected before the experiment. Statistical analysis was performed in the same manner as in Experimental Example 6 described above. Table 17 shows the results of analysis of variance (ANOVA) at the genus level before and after use of the compositions in the examples.

[0140] [Table 17]

[0141] Two weeks after using the compositions according to the examples, statistically significant changes were observed in the bacterial flora of 10 genera present on the skin. As shown in Table 17, two weeks after using the compositions according to Examples 4 and 5 of the present invention, Staphylococcus and Cutibacterium genera increased statistically significantly, while Ehrlichia, Sphingomonas, and other genera known to induce infections decreased statistically significantly after two weeks of use, confirming a statistically significant improvement in the bacterial flora on the skin. Furthermore, measurements of bacterial flora diversity (β-diversity) showed a statistically significant increase, confirming a statistically significant improvement in the bacterial flora on the skin.

[0142] The present invention is not limited to the embodiments described above, and may further include combinations of the above embodiments or combinations of at least one of the above embodiments with known technology as other embodiments.

[0143] Although the present invention has been described in detail above with reference to specific examples, this is merely for the purpose of illustrating the present invention, and it is clear that the present invention is not limited thereto, and that modifications and improvements can be made within the technical concept of the present invention by those with ordinary skill in the art.

[0144] All mere modifications and alterations of the present invention fall within the scope of the present invention, and the specific scope of protection of the present invention will be made clear by the appended claims.

Claims

1. A cosmetic composition for improving scalp health, characterized by containing polysaccharides, yeast extract, and fermented strain products as active ingredients, The aforementioned material has probiotic properties, The aforementioned yeast extract includes a beer-derived yeast extract. The aforementioned beer-derived yeast extract is included in an amount of 0.00001 to 10% by weight based on 100% by weight of the cosmetic composition. A composition characterized by being one of the following: for soothing the scalp, for improving scalp oiliness, for preventing hair loss, and for improving hair growth, and also for improving the microbial flora residing on the scalp.

2. The aforementioned polysaccharides are The scalp-improving cosmetic composition according to claim 1, characterized in that it is one or more selected from the group consisting of inulin, beta-glucan, and maltodextrin.

3. The aforementioned yeast extract is The scalp-improving cosmetic composition according to claim 1, further comprising truffle-derived yeast extract.

4. The aforementioned strains possessing probiotic properties are The scalp-improving cosmetic composition according to claim 1, characterized in that it is one or more strains selected from the group consisting of Lactobacillus strains and Bifidobacterium strains.

5. The scalp-improving cosmetic composition according to claim 4, characterized in that the material comprises one or more selected from the group consisting of fermented solubles and fermented filtrates.

6. The scalp-improving cosmetic composition according to claim 1, characterized by containing truffle-derived yeast extract, inulin, beta-glucan, maltodextrin, the aforementioned beer-derived yeast extract, Lactobacillus fermentation lysate, and Bifidobacterium fermentation lysate as active ingredients.

7. The scalp-improving cosmetic composition according to claim 6, characterized by containing truffle-derived yeast extract, inulin, beta-glucan, maltodextrin, the aforementioned beer-derived yeast extract, Lactobacillus fermentation lysate, and Bifidobacterium fermentation lysate, each in the same weight ratio.

8. The scalp-improving cosmetic composition according to claim 6, characterized in that it contains, in an amount of 0.00001 to 10% by weight, one or more active ingredients selected from the group consisting of truffle-derived yeast extract, inulin, beta-glucan, maltodextrin, the aforementioned beer-derived yeast extract, Lactobacillus ferment lysate, and Bifidobacterium ferment lysate, based on 100% by weight of the cosmetic composition.

9. The aforementioned cosmetic composition is A cosmetic composition for improving the scalp according to claim 1, characterized by promoting the growth of beneficial bacteria residing on the scalp and suppressing the growth of harmful bacteria.

10. The aforementioned beneficial bacterium is Staphylococcus epidermidis. The scalp-improving cosmetic composition according to claim 9, characterized in that the harmful bacterium is Staphylococcus aureus.

11. The skin-improving cosmetic composition according to claim 1, characterized in that the composition is a hair tonic, hair conditioner, hair essence, hair lotion, hair nourishing lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nourishing cream, hair moisturizing cream, hair massage cream, hair wax, hair aerosol, hair pack, hair nourishing pack, hair soap, hair cleansing foam, hair oil, hair drying agent, hair preservation agent, hair dye, hair waving agent, hair bleaching agent, hair gel, hair glaze, hair dresser, hair lacquer, hair moisturizer, hair mousse, or hair spray.

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