Beauty care method using combination of high-frequency electrical stimulation and cosmetic

Abstract

The present disclosure relates to a beauty care method for applying moisture or firmness to skin. More specifically, this beauty care method includes combining a beauty care composition containing a plurality of components and high-frequency waves, and applying said combination to the skin to influence gene expression of skin cells, etc. The method of the present disclosure can be used in order to enhance the production of hyaluronic acid, collagen, and elastin.

Description

Beauty method using high frequency electrical stimulation and cosmetics in combination

[0001] The present invention relates to a beauty method using high frequency waves, a beauty composition and a beauty method using high frequency waves, and a method for operating a high frequency beauty treatment device.

[0002] Radio-frequency (RF) beauty treatments are known that can improve wrinkles and sagging skin by applying radio-frequency (RF) current to the skin of the face, hands, and feet. When RF current is applied to the skin, the temperature of the dermis and subcutaneous tissue rises. This heating effect is expected to promote blood flow and promote collagen regeneration in the dermis by thermally damaging the collagen in the dermis. In recent years, various beauty treatment devices utilizing such RF currents have been proposed (see, for example, Patent Document 1).

[0003] JP 2018-489 A

[0004] However, conventional radio frequency beauty treatments promote blood circulation and collagen regeneration by heating the dermis and subcutaneous tissue with radio frequency waves, which is a temporary measure.With the increasing awareness of health in recent years, there is a demand for more fundamental improvements in function at the physical constitution level.

[0005] An object of the present invention is to provide a cosmetic method based on applying high frequency waves to the skin. Another object of the present invention is to provide a cosmetic method based on applying high frequency waves to the skin and a cosmetic composition containing multiple ingredients.

[0006] According to one aspect of the present invention, there is provided a radio frequency cosmetic treatment device for use in a cosmetic treatment method comprising: 1) applying to the skin a composition containing hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, Pinus sylvestris cone extract, and / or rose water; and 2) applying radio frequency to the skin, the radio frequency cosmetic treatment device comprising a pair of electrodes and a control circuit that applies a radio frequency voltage to the pair of electrodes.

[0007] Furthermore, according to one aspect of the present invention, there is provided a high-frequency cosmetic treatment device for use in a cosmetic method comprising: 1) applying to the skin a composition containing hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, Pinus sylvestris cone extract, and / or rose water; and 2) applying high frequency to the skin, the high-frequency cosmetic treatment device having a pair of electrodes and a control circuit that applies high-frequency voltage to the pair of electrodes, and configured to apply to the skin a high-frequency voltage that promotes the hyaluronic acid synthesis ability of fibroblasts.

[0008] Furthermore, according to one aspect of the present invention, there is provided a radio frequency cosmetic treatment device for use in a cosmetic method comprising: 1) applying to the skin a composition containing hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, Pinus sylvestris cone extract, and / or rose water; and 2) applying radio frequency to the skin, the radio frequency cosmetic treatment device having a pair of electrodes and a control circuit that applies radio frequency voltage to the pair of electrodes, and configured to apply to the skin a radio frequency voltage that promotes the collagen and elastin synthesis ability of fibroblasts.

[0009] Furthermore, according to one aspect of the present invention, there is provided a radio frequency cosmetic treatment device for use in a cosmetic method comprising: 1) applying to the skin a composition containing hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, Pinus sylvestris cone extract, and / or rose water; and 2) applying radio frequency to the skin, the radio frequency cosmetic treatment device having a pair of electrodes and a control circuit that applies a radio frequency voltage to the pair of electrodes, and configured to apply to the skin a radio frequency voltage that promotes the respiratory activity of stem cells.

[0010] Furthermore, according to one aspect of the present invention, there is provided a cosmetic composition for use in combination with a radio frequency cosmetic treatment device, the cosmetic composition comprising hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, Pinus sylvestris cone extract, and / or rose water.

[0011] Furthermore, according to one aspect of the present invention, there is provided a cosmetic composition containing hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, Pinus sylvestris cone extract, and / or rose water for use in combination with a high-frequency cosmetic treatment device to promote the ability of fibroblasts to synthesize hyaluronic acid.

[0012] Furthermore, according to one aspect of the present invention, there is provided a cosmetic composition containing hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, Pinus sylvestris cone extract, and / or rose water for use in combination with a high-frequency cosmetic treatment device to promote the collagen and elastin synthesis ability of fibroblasts.

[0013] Furthermore, according to one aspect of the present invention, there is provided a cosmetic composition comprising hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, Pinus sylvestris cone extract, and / or rose water for use in combination with a radiofrequency cosmetic treatment device to promote respiratory activity of stem cells.

[0014] Furthermore, according to one aspect of the present invention, there is provided a beauty kit including a beauty composition containing hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, Pinus sylvestris cone extract, and / or rose water, and a high-frequency beauty treatment device.

[0015] Furthermore, according to one aspect of the present invention, there is provided a beauty kit for promoting the hyaluronic acid synthesis ability of fibroblasts, comprising a beauty composition containing yeast extract, inositol, cherry leaf extract, cassia bark extract, pine extract, and / or rose water, and a high-frequency beauty treatment device.

[0016] Furthermore, according to one aspect of the present invention, there is provided a beauty kit for promoting the collagen and elastin synthesis ability of fibroblasts, comprising a beauty composition containing hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, Pinus sylvestris cone extract, and / or rose water, and a high-frequency beauty treatment device.

[0017] Furthermore, according to one aspect of the present invention, there is provided a cosmetic kit for promoting the respiratory activity of stem cells, comprising a cosmetic composition containing hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, Pinus sylvestris cone extract, and / or rose water, and a high-frequency cosmetic treatment device.

[0018] One aspect of the present invention provides a cosmetic method comprising: 1) applying to the skin a composition containing hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, Pinus sylvestris cone extract, and / or rose water; and 2) applying radiofrequency waves to the skin. Another aspect of the present invention provides a cosmetic method comprising applying radiofrequency waves to the skin to promote the ability of fibroblasts to synthesize hyaluronic acid.

[0019] Furthermore, according to one aspect of the present invention, there is provided a method for promoting the ability of fibroblasts to synthesize hyaluronic acid, comprising: 1) applying to the skin a composition containing hydrolyzed yeast, inositol, cherry leaf extract, Pinus sylvestris cone extract, cassia bark extract, and / or rose water; and 2) applying radio frequency to the skin.

[0020] Furthermore, according to one aspect of the present invention, there is provided a method for promoting the collagen and elastin synthesis ability of fibroblasts, comprising: 1) applying to the skin a composition comprising hydrolyzed yeast, inositol, cherry leaf extract, Pinus sylvestris cone extract, cassia bark extract, and / or rose water; and 2) applying radio frequency to the skin.

[0021] Furthermore, according to one aspect of the present invention, there is provided a method for promoting the respiratory activity of stem cells, comprising: 1) applying to the skin a composition comprising hydrolyzed yeast, inositol, cherry leaf extract, Pinus sylvestris cone extract, cassia bark extract, and / or rose water; and 2) applying radio frequency to the skin.

[0022] Furthermore, according to one aspect of the present invention, there is provided a method for promoting the ability of fibroblasts to synthesize hyaluronic acid, which comprises applying high frequency to the skin.

[0023] Furthermore, according to one aspect of the present invention, there is provided a method for operating a high-frequency cosmetic treatment device having a pair of electrodes and a control circuit that applies a high-frequency voltage to the pair of electrodes, the method comprising: applying a high-frequency voltage to the pair of electrodes by the control circuit that promotes the hyaluronic acid synthesis ability of fibroblasts; and applying the high-frequency voltage while the pair of electrodes is in contact with skin to which a composition containing hydrolyzed yeast, inositol, cherry leaf extract, Pinus sylvestris cone extract, cassia bark extract, and / or rose water has been applied.

[0024] Furthermore, according to one aspect of the present invention, there is provided a method for operating a high-frequency cosmetic treatment device having a pair of electrodes and a control circuit that applies a high-frequency voltage to the pair of electrodes, the method comprising: applying a high-frequency voltage to the pair of electrodes by the control circuit that promotes the collagen and elastin synthesis ability of fibroblasts; and applying the high-frequency voltage while the pair of electrodes is in contact with skin to which a composition containing hydrolyzed yeast, inositol, cherry leaf extract, Pinus sylvestris cone extract, cassia bark extract, and / or rose water has been applied.

[0025] Furthermore, according to one aspect of the present invention, there is provided a method for operating a radio-frequency cosmetic treatment device having a pair of electrodes and a control circuit that applies a radio-frequency voltage to the pair of electrodes, the method comprising: applying a radio-frequency voltage to the pair of electrodes by the control circuit that promotes the respiratory activity of stem cells; and applying the radio-frequency voltage while the pair of electrodes are in contact with skin to which a composition containing hydrolyzed yeast, inositol, cherry leaf extract, Pinus sylvestris cone extract, cassia bark extract, and / or rose water has been applied.

[0026] Furthermore, according to one aspect of the present invention, there is provided a method for operating a high-frequency cosmetic treatment device having a pair of electrodes and a control circuit that applies a high-frequency voltage to the pair of electrodes, the method including applying a high-frequency voltage to the pair of electrodes by the control circuit that promotes the hyaluronic acid synthesis ability of fibroblasts, and the high-frequency voltage is applied while the pair of electrodes are in contact with the skin.

[0027] (Definition) In this specification, "application of high frequency" and "applying high frequency" mean passing a high frequency current through an object by applying a high frequency voltage between a pair of electrodes arranged in contact with the object at a distance from each other.

[0028] Furthermore, the word "about" used when indicating a numerical value means that the value modified by this word includes values ​​within 5% of the value.

[0029] According to the present invention, cosmetic effects can be achieved by promoting the ability of skin cells to synthesize hyaluronic acid, promote the ability to synthesize collagen and elastin, and promote the respiratory activity of stem cells.

[0030] 1 is a block diagram showing one embodiment of a high-frequency device that can be used in the present invention. FIG. 1 is a graph showing the results of analysis of HAS2 gene expression levels in cases where no treatment (control) and each of the indicated treatments were performed. FIG. 2 is a graph showing the results of analysis of COL1A2 gene expression levels in cases where no treatment (control) and each of the indicated treatments were performed. FIG. 3 is a graph showing the results of analysis of COL1A2 gene expression levels in cases where treatment was performed with the cosmetic composition according to the present disclosure and each of the components alone. FIG. 4 is a graph showing the results of analysis of elastin gene expression levels in cases where no treatment (control) and each of the indicated treatments were performed. FIG. 5 is a graph showing the results of measurement of respiratory activity per cell of human mesenchymal stem cells in cases where no treatment (control) and each of the indicated treatments were performed.

[0031] The present invention is based on the inventors' new discovery that applying a composition containing hydrolyzed yeast, inositol, cherry blossom leaf extract, sylvestris cone extract, cassia bark extract, and / or rose water in combination with radiofrequency (RF) to the skin significantly promotes the hyaluronic acid synthesis ability of fibroblasts, the collagen and elastin synthesis ability of fibroblasts, and the respiratory activity of stem cells. One aspect of the present invention provides a cosmetic method comprising: 1) applying a composition containing hydrolyzed yeast, inositol, cherry blossom leaf extract, cassia bark extract, sylvestris cone extract, and / or rose water to the skin; and 2) applying radiofrequency to the skin. The inventors have also found that applying radiofrequency (RF) alone to the skin promotes the hyaluronic acid synthesis ability of fibroblasts. Therefore, one aspect of the present invention relates to a cosmetic method comprising applying radiofrequency to the skin to promote the hyaluronic acid synthesis ability of fibroblasts. Increased hyaluronic acid, collagen, and elastin also affect skin moisture and firmness, and application of the cosmetic method of the present invention contributes to providing skin moisture and firmness. Furthermore, promotion of stem cell respiratory activity is thought to contribute to stem cell activation. Furthermore, some aspects of the present invention also relate to high-frequency cosmetic treatment devices, cosmetic compositions, and cosmetic kits used in carrying out the cosmetic method.

[0032] The plant extract used in the present invention can be extracted from plant materials by, for example, methods known to those skilled in the art. Such plant extracts can be obtained by any method known to those skilled in the art, such as solvent extraction methods or methods including crushing and squeezing steps. The plant part to be extracted is washed with water, if necessary, to remove foreign matter, and then extracted either as is or dried, and then shredded or crushed as necessary, and then contacted with an extraction solvent. Extraction can be carried out by contacting with an extraction solvent using conventional methods such as immersion, but supercritical extraction or steam distillation may also be used.

[0033] When performing extraction, the plant body can be used as is, but crushing it into granules or powder before extraction allows for extraction of active ingredients under milder conditions in a shorter time with higher extraction efficiency. The extraction temperature is not particularly limited and can be set appropriately depending on the particle size of the crushed material, the type of solvent, etc. It is usually set within the range from room temperature to the boiling point of the solvent. The extraction time is also not particularly limited and can be set appropriately depending on the particle size of the crushed material, the type of solvent, the extraction temperature, etc. Furthermore, during extraction, stirring may be performed, the mixture may be left to stand without stirring, or ultrasound may be applied.

[0034] Examples of extraction solvents include water; lower alcohols such as methanol, ethanol, and propanol; higher alcohols such as oleyl alcohol, stearyl alcohol, and octyldodecanol; polyhydric alcohols such as ethylene glycol, 1,3-propanediol, 1,3-butylene glycol, and glycerin; esters such as ethyl acetate, butyl acetate, methyl propionate, and glyceryl trioctanoate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether, isopropyl ether, and hydrocarbon solvents such as n-hexane, toluene, and chloroform. These solvents can be used alone or in combination. Preferred solvents are polar solvents such as water, lower alcohols, and liquid polyhydric alcohols, and more preferably water or lower alcohols such as methanol, ethanol, and 1,3-butylene glycol. The lower alcohol may be, for example, a water-containing lower alcohol, in which case the water content may be, for example, 0 to 10 v / v%, 10 to 40 v / v%, 20 to 30 v / v%, 30 to 50 v / v%, 50 to 80 v / v%, 80 to 99.5 v / v%, etc. The lower alcohol may be, for example, a C1 to C5 lower alcohol. These solvents may be used alone or in combination. Furthermore, an enzyme may be added to the solvent to perform the extraction treatment.

[0035] When a mixed solvent is used, the mixing ratio (volume ratio) is preferably in the range of 1:1 to 25:1 for a mixed solvent of water and ethyl alcohol, 1:1 to 15:1 for a mixed solvent of water and glycerin, or 1:1 to 15:1 for a mixed solvent of water and 1,3-propanediol or 1,3-butylene glycol.

[0036] When preparing an extract, the pH is not particularly limited, but is generally preferably in the range of pH 3 to 9. If necessary, the extraction solvent may be blended with an alkalinity adjuster such as sodium hydroxide, sodium carbonate, or potassium hydroxide, or an acidity adjuster such as citric acid, hydrochloric acid, phosphoric acid, or sulfuric acid to adjust the pH to the desired level.

[0037] The extraction conditions, such as the extraction temperature and extraction time, vary depending on the type and pH of the solvent used and are not limited. For example, when water, 1,3-butylene glycol, or a mixture of water and 1,3-butylene glycol is used as the solvent, the extraction temperature may be in the range of 0°C to 90°C, and the extraction time may be 0.5 hours to 7 days.

[0038] By such an extraction procedure, the active ingredient is extracted and dissolved in the solvent. The solvent containing the extract may be used as is, or may be subjected to conventional purification treatments such as sterilization, washing, filtration, bleaching, and deodorization before use. It may also be concentrated or diluted as necessary before use. Furthermore, the solvent may be completely evaporated to form a solid (dried product), or the dried product may be redissolved in any solvent before use.

[0039] If necessary, the extracted part may be subjected to hydrolysis prior to or in parallel with the extraction process. This may improve the skin irritation, efficacy, storage stability, etc. of the extract, allowing for more effective use of the extract. In addition, the squeezed liquid obtained by squeezing the raw plant material contains the same active ingredients as the extract, so the squeezed liquid can be used instead of the extract.

[0040] The frequency of the radiofrequency waves applied to the skin may be in the range of 0.5 MHz to 4 MHz, for example, 0.5 MHz, 1 MHz, 2 MHz, 3 MHz, or 4 MHz, or any frequency within a range defined by any of the above frequencies (e.g., the range of 1 to 4 MHz). In some embodiments of the present invention, the frequency is about 1 MHz.

[0041] In some embodiments of the present invention, a composition applied to the skin in combination with radiofrequency radiation contains hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, Scots pine cone extract, and / or rose water. Specifically, one aspect of the present invention relates to a cosmetic composition containing hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, Scots pine cone extract, and rose water for use in combination with a radiofrequency cosmetic treatment device, particularly a cosmetic composition for promoting the ability of fibroblasts to synthesize hyaluronic acid, promoting the ability of fibroblasts to synthesize collagen and elastin, and promoting the respiratory activity of stem cells. The composition disclosed herein may contain at least one, at least two, at least three, at least four, at least five, or all six of the components selected from the group consisting of hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, Scots pine cone extract, and rose water. When each component is blended into a cosmetic or quasi-drug, the blending amount can be, for example, 0.00001 to 0.5% by mass for each component.

[0042] For example, the hydrolyzed yeast may be ceravure (yeast extract) available from Iwaki Co., Ltd., and the blending amount may be 0.00001 to 0.0001% by mass. The inositol may be inositol available from Tsuno Rice Fine Chemicals Co., Ltd., and the blending amount may be 0.0005 to 0.005% by dry mass. The cherry leaf extract may be cherry leaf extract available from Ichimaru Falcos Co., Ltd., and the blending amount may be 0.001 to 0.01% by mass. The cassia bark extract may be cinnamon extract ET30 available from Iwaki Co., Ltd., and the blending amount may be 0.00001 to 0.0001% by mass. The pine cone extract may be pine extract BG available from Ichimaru Falcos Co., Ltd., and the blending amount may be 0.0001 to 0.001% by mass. As the rose water, Bulgarian rose water available from Toyotama Fragrance Co., Ltd. can be used, and the blending amount can be 0.05 to 0.5% by mass.

[0043] The composition to be applied to the skin in combination with high frequency may further contain other drugs, various extracts, etc. Examples of applicable drugs and various extracts include retinol derivatives, xylitol, hyaluronic acid, glycerin, okra extract, Chinese musk extract, burnet extract, dipotassium glycyrrhizinate, ginkgo leaf extract, Chinese angelica tree extract, coix seed extract, licorice extract, Panax ginseng extract, Sophora flavescens extract, star fruit leaf extract, carrot extract, Herb extract, neem leaf extract, saffron extract, cinchona extract, comfrey extract, thyme extract, winter strawberry, yomena, Mube, Ryukyu strawberry, Indian yomena, Lithocarpus edulis, Scutellaria baicalensis extract, rosemary extract, apricot kernel grain, gentian extract, peppermint powder, taiso extract, hop extract, kiwi extract, Roman chamomile extract, apple extract, hawthorn extract, turmeric extract, bayberry, myrtaceae, and oak.

[0044] The cosmetic composition according to the present disclosure may be in the form of an emulsion, cream, serum, lotion, pack, facial cleanser, soap, body soap, shampoo, etc., and may be in various forms such as liquid, emulsion, cream, solid, sheet, spray, gel, foam, etc. However, the forms that the present invention can take are not limited to these forms. In addition, the composition according to the present disclosure may contain additives such as excipients, colorants, preservatives, thickeners, binders, disintegrants, dispersants, stabilizers, gelling agents, antioxidants, surfactants, preservatives, pH adjusters, oils, water, alcohols, chelating agents, silicones, UV absorbers, moisturizers, fragrances, various medicinal ingredients, preservatives, neutralizing agents, etc.

[0045] The application of the cosmetic composition and the high frequency radiation to the skin can be carried out in any order and at any time interval. For example, the application of the high frequency radiation can be carried out almost simultaneously with or immediately after the application of the composition, for example, within 5 seconds, 10 seconds, 30 seconds, 1 minute, 3 minutes, 5 minutes, 10 minutes, 15 minutes, 30 minutes, 45 minutes, 60 minutes, 2 hours, 3 hours, or 6 hours after the application of the composition.

[0046] A high frequency device can be used to apply high frequency waves to the skin. One form of the high frequency device will be described with reference to FIG.

[0047] The high-frequency device 1 includes an electrode unit 10 and a control circuit 20. The electrode unit 10 includes a first electrode 10a and a second electrode 10b connected to the control circuit 20. The electrode unit 10 is provided with a contact surface that contacts the user's skin, and the first electrode 10a and the second electrode 10b are arranged on this contact surface at a distance from each other, so that the first electrode 10a and the second electrode 10b can be easily brought into contact with the skin at a distance from each other. The first electrode 10a and the second electrode 10b may be connected to the control circuit 20 via a wiring cable rather than via the contact surface, allowing the first electrode 10a and the second electrode 10b to be arranged freely.

[0048] The control circuit 20 may include a power supply unit, a voltage control unit, and a resistance measurement unit. The power supply unit applies a high-frequency voltage (a voltage indicating high frequency) between the first electrode 10a and the second electrode 10b. The power supply unit includes an oscillator circuit that generates the high-frequency voltage and a booster circuit that boosts the oscillated voltage, and is configured to apply a high-frequency voltage of, for example, approximately 0.5 MHz to 4 MHz between the first electrode 10a and the second electrode 10b. By applying a high-frequency voltage between the first electrode 10a and the second electrode 10b while the first electrode 10a and the second electrode 10b are in contact with the user's skin, a high-frequency current can be passed through the skin. The current load generated by the high-frequency device can be applied to the skin for, for example, 1 second to 5 minutes.

[0049] The voltage control unit controls the power supply unit to control the high-frequency voltage applied by the power supply unit. The voltage control unit also uses the measurement results from the resistance measurement unit to control the voltage. The resistance measurement unit measures the resistance value (electrical resistance value) between the first electrode 10a and the second electrode 10b when the first electrode 10a and the second electrode 10b are in contact with the user's skin.

[0050] This resistance value varies depending on the condition of the user's skin and the type of external skin preparation, such as lotion, applied to the skin. For example, it is known that when a high-frequency current is applied to human skin, the skin heats up and the impedance inside the skin decreases as the skin temperature rises. Therefore, as the skin temperature rises, the current flowing between the first electrode 10a and the second electrode 10b increases, and the measured resistance value decreases. The resistance measurement unit measures the resistance value at a predetermined time interval (e.g., every 0.5 seconds) and supplies the measured resistance value to the voltage control unit each time.

[0051] The voltage control unit controls the high frequency voltage according to the resistance value measured by the resistance measurement unit, for example, so that the higher the measured resistance value, the higher the high frequency voltage (the larger the amplitude of the high frequency voltage).

[0052] The lower limit of the high-frequency voltage applied between the first electrode 10a and the second electrode 10b is preferably 50 Vpp or more, more preferably 70 Vpp or more, in peak-to-peak voltage. The upper limit of the high-frequency voltage applied between the first electrode 10a and the second electrode 10b is preferably 100 V or less, more preferably 90 Vpp or less, in peak-to-peak voltage. The applied high-frequency voltage can be, for example, 88 Vpp.

[0053] The high-frequency voltage may be applied continuously or in pulses. In the case of pulse application, the application (ON) and non-application (OFF) of the high-frequency voltage are repeated periodically. The period of the repeated pulse application may be 0.1 to 2 seconds, for example, 0.1, 0.2, 0.3, 0.5, 0.8, 1, or 1.2 seconds. The duty ratio, which is the ON time ratio of the high-frequency voltage in the pulse application, is preferably in the range of 10% to 99%, and more preferably in the range of 20% to 80%. The duty ratio may be, for example, 40%.

[0054] The application conditions of the high frequency, such as the high frequency voltage and duty ratio, may be user-configurable. To enable the user to set the application conditions of the high frequency, the high frequency device may have an input device operated by the user. Any device, such as a switch, may be used as the input device. When the application conditions of the high frequency are user-configurable, the control circuit 20 receives input operations through the input device and controls parameters such as the high frequency voltage, whether the application is continuous or pulsed, and the period and duty ratio in the case of pulsed application, according to the received input operations. When setting the application conditions of the high frequency, each parameter may be independently configurable, or multiple combinations of the high frequency voltage, period, and duty ratio for pulsed application may be preset in the control circuit 20, and the user may select one of the preset combinations.

[0055] By using the high-frequency device described above, high frequencies can be easily applied to the user's skin. The high-frequency device can be operated as follows: First, the first electrode 10a and the second electrode 10b are placed in contact with the user's skin with a gap between them. Next, the control circuit 20 applies a high-frequency voltage between the first electrode 10a and the second electrode 10b placed in contact with the user's skin so as to promote the synthesis of hyaluronic acid, collagen, and elastin in the skin, and to promote the respiratory activity of stem cells.

[0056] One aspect of the present invention relates to a radiofrequency device for use in combination with applying to the skin a composition comprising hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, Pinus sylvestris cone extract, and / or rose water, particularly for use in promoting the skin's ability to synthesize hyaluronic acid, promote collagen and elastin synthesis, or promote stem cell respiratory activity.

[0057] One aspect of the present invention relates to a cosmetic kit including a cosmetic composition according to the present disclosure and a radio-frequency cosmetic treatment device. The cosmetic kit can be used to promote the skin's ability to synthesize hyaluronic acid, promote collagen and elastin synthesis, or promote stem cell respiratory activity. The cosmetic composition is contained in, for example, a bottle. The cosmetic kit may also include instructions on how to use the composition and the radio-frequency treatment device.

[0058] [Confirmation of Improvement in Stability of Dermal Stem Cells by Application of High Frequency] Hereinafter, an experiment conducted by the inventors to confirm the effect of application of high frequency on cells will be described.

[0059] [Confirmation of the effect of promoting hyaluronic acid synthesis] One million normal adult human dermal fibroblasts (36-year-old female, unexposed area) cryopreserved at passage 9 were seeded onto a 100 mm culture dish (cat. no. 353003, CORNING) and pre-cultured in DMEM medium containing 10% FBS (cat. no. 11885084, Invitrogen). After 4 days of culture, the cells were detached according to standard procedures and seeded at 16,000 cells onto a 35 mm culture dish for current loading. The medium used was also DMEM medium containing 10% FBS. The 35 mm culture dish for current loading was disinfected with 70% ethanol and rinsed twice with DPBS(-) immediately before use. One day after seeding onto the culture dishes for current loading, the following groups were prepared: a no-addition group, a group with the compounded ingredients (a composition containing hydrolyzed yeast, inositol, leaf extract, cassia bark extract, Pinus sylvestris cone extract, and rose water; Table 1) added, a group with high-frequency current loading for 30 seconds, and a group with high-frequency current loading for 30 seconds and the compounded ingredients added, and each group was treated.

[0060] To apply the high frequency, a beauty device (manufactured by YAMAN) that generates a 1MHz high frequency current was used. The output was turned on and off repeatedly at 0.4 / 0.6 [sec], and the AC voltage applied between the electrodes was 88 [Vpp: Voltage peak to peak].

[0061] The cells were returned to a CO2 incubator and further cultured overnight, after which total RNA was isolated using an RNeasy Mini Kit (cat. no. 74106, Qiagen). RNA concentration was measured using a NanoDrop kit, and cDNA was synthesized from 1 μg of RNA using a SuperScript VILO cDNA Synthesis Kit (cat. no. 11754, Invitrogen). HAS2 gene expression was quantitatively analyzed using StepOne (Applied Bioscience) with SYBR Green (cat. no. 11733046, Invitrogen). The primers used in the reaction are listed in Table 2. GAPDH was used as the housekeeping gene.

[0062]

[0063]

[0064] HAS2 gene expression levels were analyzed in the untreated (control) and each treatment group. Gene quantity was normalized using the housekeeping gene GAPDH, and HAS2 gene expression levels relative to the control are shown in Table 3 and Figure 2. HAS2 gene expression levels in human fibroblasts in each treatment group tended to increase compared to the control, and in particular, treatment with a radiofrequency cosmetic device alone or simultaneous application of the compounded ingredients and a radiofrequency cosmetic device increased HAS2 gene expression in human fibroblasts with a statistically significant difference.

[0065]

[0066] These results demonstrate that the application of a radiofrequency beauty device alone, or the simultaneous application of a beauty composition according to the present disclosure and a radiofrequency beauty device, significantly increases the expression of the HAS2 gene in human fibroblasts. Because HAS2 is the main synthase gene that regulates hyaluronic acid synthesis in human dermal fibroblasts, the application of a radiofrequency beauty device alone, or the simultaneous application of a beauty composition according to the present disclosure and a radiofrequency beauty device, has the effect of promoting the hyaluronic acid synthesis ability of human dermal fibroblasts.

[0067] [Confirmation of collagen synthesis promotion effect] One million cryopreserved normal human dermal fibroblasts (cat. no. KF-4009, lot no. 05884, KURABO) at passage 6 were seeded onto a 100 mm culture dish (cat. no. 353003, CORNING) and pre-cultured in DMEM medium containing 10% FBS (cat. no. 11885084, Invitrogen). After one day of culture, the cells were detached according to standard procedures and seeded at 30,000 cells onto a 35 mm culture dish for electrical current loading. The culture medium used was also DMEM medium containing 10% FBS. The 35 mm culture dish for electrical current loading was disinfected with 70% ethanol and rinsed twice with DPBS(-) immediately before use. One day after seeding onto the culture dishes for current loading, the following groups were prepared: a no-addition group, a group with the compounded ingredients (a composition containing hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, Pinus sylvestris cone extract, and rose water; Table 1) added, a group with high-frequency current loading for 3 minutes, and a group with high-frequency current loading for 3 minutes and the compounded ingredients added, and each group was treated.

[0068] To apply the high frequency, a beauty device (manufactured by YA-MAN) generating a 1MHz high frequency current was used. The output was turned on and off repeatedly at 0.4 / 0.6 [sec], and the AC voltage applied between the electrodes was 88 [Vpp: Voltage peak to peak].

[0069] The cells were returned to a CO2 incubator and cultured for an additional 72 hours, after which total RNA was collected using an RNeasy Mini Kit (cat. no. 74106, Qiagen). RNA concentration was measured using a NanoDrop kit, and cDNA was synthesized from 1 μg of RNA using a SuperScript VILO cDNA Synthesis Kit (cat. no. 11754, Invitrogen). Collagen type I alpha 2 chain (COL1A2) gene expression was quantitatively analyzed using StepOne (Applied Bioscience) with SYBR Green (cat. no. 11733046, Invitrogen). The primers used in the reaction are listed in Table 2. GAPDH was used as a housekeeping gene.

[0070]

[0071] The expression level of the COL1A2 gene was analyzed in the untreated (control) and each treatment group. Gene levels were normalized using the housekeeping gene GAPDH, and the COL1A2 gene expression levels relative to the control are shown in Table 5 and Figure 3. The expression level of the COL1A2 gene in human fibroblasts in each treatment group tended to increase compared to the control, but a statistically significant increase was observed only when the cosmetic composition according to the present disclosure and the radiofrequency cosmetic device were simultaneously applied.

[0072]

[0073] 4 further shows the analysis results of COL1A2 gene expression levels when treated with the cosmetic composition (compounded ingredients) according to the present disclosure and each of the individual ingredients (hydrolyzed yeast, cherry leaf extract, Bulgarian rose water, inositol, Pinus sylvestris cone extract, and cassia bark extract). These results demonstrate that the cosmetic composition (compounded ingredients) according to the present disclosure has a superior effect to each of the individual ingredients alone.

[0074] These results demonstrate that simultaneous application of the cosmetic composition according to the present disclosure and a radio-frequency cosmetic device significantly increases the expression of the COL1A2 gene in human fibroblasts. The COL1A2 gene encodes one of the components of type I collagen, which accounts for a large proportion of collagen in the human dermis. Therefore, simultaneous application of the cosmetic composition according to the present disclosure and a radio-frequency cosmetic device was shown to promote the collagen synthesis ability of human dermal fibroblasts.

[0075] [Confirmation of the effect of promoting elastin synthesis] One million cryopreserved normal human dermal fibroblasts (cat. no. KF-4009, lot no. 05884, KURABO) at passage 6 were seeded onto 100 mm culture dishes (cat. no. 353003, CORNING) and pre-cultured in DMEM medium containing 10% FBS (cat. no. 11885084, Invitrogen). After one day of culture, the cells were detached according to standard procedures and seeded at 30,000 cells onto 35 mm culture dishes for electrical current loading. The culture medium used was also DMEM medium containing 10% FBS. The 35 mm culture dishes for electrical current loading were disinfected with 70% ethanol and rinsed twice with DPBS(-) immediately before use. One day after seeding onto the culture dishes for current loading, the following groups were prepared: a no-addition group, a group with the compounded ingredients (a composition containing hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, Pinus sylvestris cone extract, and rose water; Table 1) added, a group with high-frequency current loading for 3 minutes, and a group with high-frequency current loading for 3 minutes and the compounded ingredients added, and each group was treated.

[0076] To apply the high frequency, a beauty device (manufactured by YAMAN) that generates a 1MHz high frequency current was used. The output was turned on and off repeatedly at 0.4 / 0.6 [sec], and the AC voltage applied between the electrodes was 88 [Vpp: Voltage peak to peak].

[0077] The cells were returned to a CO2 incubator and cultured for an additional 72 hours, after which total RNA was collected using an RNeasy Mini Kit (cat. no. 74106, Qiagen). RNA concentration was measured using a NanoDrop kit, and cDNA was synthesized from 1 μg of RNA using a SuperScript VILO cDNA Synthesis Kit (cat. no. 11754, Invitrogen). Elastin gene expression was quantitatively analyzed using StepOne (Applied Bioscience) with SYBR Green (cat. no. 11733046, Invitrogen). The primers used in the reaction are listed in Table 2. GAPDH was used as the housekeeping gene.

[0078]

[0079] Elastin gene expression levels were analyzed in the untreated (control) and each treatment group. Gene levels were normalized using the housekeeping gene GAPDH, and the elastin gene expression levels relative to the control are shown in Table 7 and Figure 5. Elastin gene expression levels in human fibroblasts in each treatment group tended to increase compared to the control, but a statistically significant increase was observed only when the cosmetic composition according to the present disclosure and the high-frequency cosmetic device were applied simultaneously.

[0080]

[0081] These results demonstrate that simultaneous application of the cosmetic composition according to the present disclosure and a radio-frequency cosmetic device significantly increases the expression of the elastin gene in human fibroblasts. Because the elastin gene encodes the main component of elastic fiber in skin, simultaneous application of the cosmetic composition according to the present disclosure and a radio-frequency cosmetic device has been shown to promote the elastin synthesis ability of human dermal fibroblasts.

[0082] [Confirmation of the effect of promoting stem cell respiratory activity] One million cryopreserved human mesenchymal stem cells (P / N 51-0070, Invitrogen) at passage 1 were seeded into a T-75 flask and pre-cultured in MesenPRO RS™ Medium (cat. no. 12746012, Thermo Fisher Scientific). After two days of culture, the cells were detached according to standard procedures and seeded at 60,000 cells into a 35 mm culture dish for electrical current loading. The medium used was Mesenchymal Stem Cell Growth Medium 2 (cat. no. C-28009, Nacalai Tesque, Inc.). The 35 mm culture dish for electrical current loading was disinfected with 70% ethanol and rinsed twice with DPBS(-) immediately before use. Following the manufacturer's recommended protocol, 500 ml of CTS™ CELLstart™ Substrate (cat. no. A1014201, Thermo Fisher Scientific) was used per culture dish for 1 hour at 37°C. One day after seeding onto the culture dishes for current application, treatments were performed in the following groups: a control group, a group treated with the compound (a composition containing hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, Pinus sylvestris cone extract, and rose water; Table 1), a group treated with high-frequency current for 1 minute, and a group treated with high-frequency current for 1 minute and the compound.

[0083] To apply the high frequency, a beauty device (manufactured by YAMAN) that generates a 1MHz high frequency current was used. The output was turned on and off repeatedly at 0.4 / 0.6 [sec], and the AC voltage applied between the electrodes was 88 [Vpp: Voltage peak to peak].

[0084] After each treatment, the cells were returned to the CO2 incubator and further cultured overnight. The respiratory activity of the human mesenchymal stem cells was then measured according to the Alamar blue (cat. no. DAL1025, Invitrogen) instruction manual. The culture medium was replaced with Mesenchymal Stem Cell Growth Medium 2 containing Alamar blue, and after 1 hour of incubation, the fluorescence intensity (excitation wavelength: 544 nm, detection wavelength: 590 nm) was measured. After the measurement, the cells were detached according to the standard method and stained for viable cells with 0.4% trypan blue solution (cat. no. T10282, Invitrogen) according to the instruction manual, and counted. All human mesenchymal stem cells were cultured in a 5% CO2, 37°C CO2 incubator.

[0085] The respiratory activity per human mesenchymal stem cell was measured by dividing the fluorescence intensity obtained from untreated (control) and each treated human mesenchymal stem cell by the number of cells. The results are shown in Table 8 and Figure 6. Compared to the control, each treatment group showed a tendency for the respiratory activity of human mesenchymal stem cells to be promoted, but only when the cosmetic composition according to the present disclosure and the radio-frequency cosmetic device were applied simultaneously, did this promotion occur with a statistically significant difference.

[0086]

[0087] These results demonstrate that the simultaneous application of the cosmetic composition according to the present disclosure and a radiofrequency cosmetic device effectively promotes the respiratory activity of human mesenchymal stem cells, which may contribute to the activation of human mesenchymal stem cells.

[0088] 1 high frequency device 10 electrode portion 10a first electrode 10b second electrode 20 control circuit

Claims

1. 1) applying to the skin a composition comprising hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, sylvestris cone extract, and / or rose water; and 2) Applying high frequency to the skin A high frequency cosmetic treatment device for use in a cosmetic method, comprising: A high frequency cosmetic treatment device comprising a pair of electrodes and a control circuit that applies a high frequency voltage to the pair of electrodes.

2. 1) applying to the skin a composition comprising hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, sylvestris cone extract, and / or rose water; and 2) Applying high frequency to the skin A high frequency cosmetic treatment device for use in a cosmetic method, comprising: a pair of electrodes and a control circuit that applies a high-frequency voltage to the pair of electrodes; A high frequency cosmetic treatment device configured to apply a high frequency voltage to the skin that promotes the ability of fibroblasts to synthesize hyaluronic acid.

3. 1) applying to the skin a composition comprising hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, sylvestris cone extract, and / or rose water; and 2) Applying high frequency to the skin A high frequency cosmetic treatment device for use in a cosmetic method, comprising: a pair of electrodes and a control circuit that applies a high-frequency voltage to the pair of electrodes; A high frequency cosmetic treatment device configured to apply a high frequency voltage to the skin that promotes the collagen and elastin synthesis ability of fibroblasts.

4. 1) applying to the skin a composition comprising hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, sylvestris cone extract, and / or rose water; and 2) Applying high frequency to the skin A high frequency cosmetic treatment device for use in a cosmetic method, comprising: a pair of electrodes and a control circuit that applies a high-frequency voltage to the pair of electrodes; A radio frequency cosmetic treatment device configured to apply a radio frequency voltage to the skin that promotes the respiratory activity of stem cells.

5. A cosmetic composition for use in combination with a radio frequency cosmetic treatment device, comprising hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, sylvestris cone extract, and / or rose water.

6. A cosmetic composition comprising hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, sylvestris cone extract, and / or rose water for use in combination with a radiofrequency cosmetic treatment device to promote the ability of fibroblasts to synthesize hyaluronic acid.

7. A cosmetic composition comprising hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, sylvestris cone extract, and / or rose water for use in combination with a radiofrequency cosmetic treatment device to promote the collagen and elastin synthesis ability of fibroblasts.

8. A cosmetic composition comprising hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, sylvestris cone extract, and / or rose water for use in combination with a radiofrequency cosmetic treatment device to promote stem cell respiratory activity.

9. a cosmetic composition comprising hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, sylvestris cone extract, and / or rose water; A high frequency beauty treatment device, Includes a beauty kit.

10. a cosmetic composition comprising hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, sylvestris cone extract, and / or rose water; A high frequency beauty treatment device, A beauty kit for use in promoting the ability of fibroblasts to synthesize hyaluronic acid, comprising:

11. a cosmetic composition comprising hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, sylvestris cone extract, and / or rose water; A high frequency beauty treatment device, A cosmetic kit for use in promoting the collagen and elastin synthesis ability of fibroblasts, comprising:

12. a cosmetic composition comprising hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, sylvestris cone extract, and / or rose water; A high frequency beauty treatment device, A beauty kit for use in promoting the respiratory activity of stem cells, comprising:

13. 1) applying to the skin a composition comprising hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, sylvestris cone extract, and / or rose water; and 2) Applying high frequency to the skin Beauty methods, including:

14. The cosmetic method according to claim 13, for promoting the ability of fibroblasts to synthesize hyaluronic acid.

15. The cosmetic method according to claim 13, for promoting the collagen and elastin synthesis ability of fibroblasts.

16. The cosmetic method according to claim 13, for promoting the respiratory activity of stem cells.

17. A cosmetic method comprising applying high frequency to the skin to stimulate the ability of fibroblasts to synthesize hyaluronic acid.

18. The cosmetic method according to claim 13, wherein the frequency of the high frequency is about 1 MHz.

19. 14. The cosmetic method according to claim 13, wherein the high frequency has a peak-to-peak voltage of 50 to 100 Vpp.

20. The cosmetic method according to claim 13, wherein the high frequency is applied in pulses with a duty ratio of 10% to 99%.

21. 21. The cosmetic method according to claim 20, wherein the pulse application period is 0.1 seconds to 2 seconds.

22. 22. The cosmetic method according to claim 21, wherein the pulse application period is 1 second.

23. The cosmetic method according to claim 13, which improves skin firmness.

24. The cosmetic method according to claim 13, which improves skin moisture.

25. 1) applying to the skin a composition comprising hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, sylvestris cone extract, and / or rose water; and 2) Applying high frequency to the skin A method for promoting the ability of fibroblasts to synthesize hyaluronic acid, comprising:

26. 1) applying to the skin a composition comprising hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, sylvestris cone extract, and / or rose water; and 2) Applying high frequency to the skin A method for promoting the collagen and elastin synthesis ability of fibroblasts, comprising:

27. 1) applying to the skin a composition comprising hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, sylvestris cone extract, and / or rose water; and 2) Applying high frequency to the skin A method for promoting the respiratory activity of stem cells, comprising:

28. A method for promoting the ability of fibroblasts to synthesize hyaluronic acid, comprising applying radiofrequency to the skin.

29. A method for operating a high frequency cosmetic treatment device having a pair of electrodes and a control circuit that applies a high frequency voltage to the pair of electrodes, comprising: applying a high-frequency voltage to the pair of electrodes by the control circuit, the high-frequency voltage promoting the ability of fibroblasts to synthesize hyaluronic acid; Including, the application of the high-frequency voltage is carried out in a state where the pair of electrodes are in contact with skin to which a composition containing hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, Pinus sylvestris cone extract, and / or rose water has been applied; A method for operating a radio frequency beauty treatment device.

30. A method for operating a high frequency cosmetic treatment device having a pair of electrodes and a control circuit that applies a high frequency voltage to the pair of electrodes, comprising: applying a high-frequency voltage to the pair of electrodes by the control circuit, the high-frequency voltage promoting the ability of fibroblasts to synthesize collagen and elastin; Including, The high-frequency voltage is applied in a state where the pair of electrodes are in contact with skin to which a composition containing hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, Pinus sylvestris cone extract, and / or rose water has been applied. A method for operating a radio frequency beauty treatment device.

31. A method for operating a high frequency cosmetic treatment device having a pair of electrodes and a control circuit that applies a high frequency voltage to the pair of electrodes, comprising: applying a high-frequency voltage to the pair of electrodes by the control circuit to promote respiratory activity of stem cells; Including, The high-frequency voltage is applied in a state where the pair of electrodes are in contact with skin to which a composition containing hydrolyzed yeast, inositol, cherry leaf extract, cassia bark extract, Pinus sylvestris cone extract, and / or rose water has been applied. A method for operating a radio frequency beauty treatment device.

32. A method for operating a high frequency cosmetic treatment device having a pair of electrodes and a control circuit that applies a high frequency voltage to the pair of electrodes, comprising: applying a high-frequency voltage to the pair of electrodes by the control circuit, the high-frequency voltage promoting the ability of fibroblasts to synthesize hyaluronic acid; Including, The high-frequency voltage is applied with the pair of electrodes in contact with the skin. A method for operating a radio frequency beauty treatment device.

33. 18. The method of claim 17, wherein the peak-to-peak voltage of the high frequency is 50 to 100 Vpp.

34. 18. The operating method according to claim 17, wherein the high-frequency voltage is applied as a pulse with a 1-second period and a duty ratio of 10 to 99%.