SNP locus combination associated with mastitis resistance traits in dairy cows, probes, a chip, and applications thereof

Abstract

The present invention belongs to the technical field of molecular breeding, and specifically relates to an SNP locus combination associated with mastitis resistance traits in dairy cows, as well as probes, a chip, and applications thereof. The SNP locus combination comprises 348 SNP loci located at specific chromosomal positions, the physical positions of which are determined based on the ARS-UCD2.0 reference genome. The present invention further provides probes and a liquid-phase chip for detecting the SNP locus combination, which can be used for early selection and breeding of mastitis resistance in dairy cows, identification of germplasm resources, kinship analysis, and pedigree correction. By means of high-throughput genotyping technology, the present invention enables rapid and accurate evaluation of innate resistance in dairy cows, and has the advantages of high detection efficiency, low cost, and suitability for large-scale population screening, thereby having important significance for improving disease-resistant breeding efficiency in dairy cows and ensuring the safe production of dairy products.