Plate and method for high throughput screening

a plate and high throughput technology, applied in the field of plates and methods for promoting tissue growth, can solve the problems of other tissues, reducing the efficiency of screening plant tissue, and consuming plant tissue in an anaerobic environment, so as to reduce workload, promote well-to-well consistency, and reduce the effect of workload

US20080102518A1Inactive Publication Date: 2008-05-01BIOLEX THERAPEUTICS INC
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
Publication Date
2008-05-01
Estimated Expiration
Not applicable · inactive patent

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Abstract

A multiple well plate and method for media exchange, including a body defining a plurality of cell wells each connected via a channel to one of a plurality of aspiration holes, is provided. The cell wells contain a porous, hydrophilic frit which is suspended on a ledge above a reservoir of fluid media and supports a tissue sample. The properties of the frit wick the fluid media upwards to supply the tissue sample with nutrients for growth and proliferation. Old media is aspirated from the wells by a liquid handling device which inserts a pipette tip into the aspiration holes. The pipette tip applies a suction pressure which draws the media out of the cell well, through the channel, into the aspiration hole and out through the pipette tip. New media is dispersed through the pipette tip and directly into the cell well.
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Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] The present application is a divisional of U.S. application Ser. No. 10 / 157,562, filed May 29, 2002, which claims the benefit of U.S. Provisional Application No. 60 / 294,430, filed May 30, 2001, both of which are hereby incorporated herein in their entirety by reference.FIELD OF THE INVENTION

[0002] The present invention relates to devices and methods for promoting the growth of tissue in experimental and production settings, more particularly the use of specialized plates to house the tissue and the cycling of media to biologically sustain the tissue. BACKGROUND OF THE INVENTION

[0003] High-throughput screening typically requires parallel processing of batches of samples, typically in multiple well plates (MWPs) of 24, 48, 96, and 384, or more, wells per plate. MWPs are standard sizes that can be used with existing high-throughput machinery, such as with robotic-controlled pipetters. Each pipetting station of a robotic controlled pipett...

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Embodiment Construction

[0024] The present invention now will be described more fully hereinafter with reference to the accompanying drawings, in which preferred embodiments of the invention are shown. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein; rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art. Like numbers refer to like elements throughout.

[0025] A multiple well plate (MWP) 10 of the present invention includes a body 12 having an upper surface 14. The body 12 defines an array of first holes, or wells 16 and an array of second aspiration holes 22, as shown in FIG. 1. A plurality of channels 28 each connect a respective one of the wells 16 to an adjacent one of the aspiration holes 22, as shown in FIG. 2. In one embodiment, disposed in each of the wells 16 is a porous, hydrophilic frit 30 wh...