PROPAGATION PROCESS OF EUCHEUM DENTICULATUM (Spiny Seaweed)

Abstract

This invention proposes a process for propagating spiny cartilage seaweed (E. denticulatum) using callus tissue culture based on a callus-embryo-synthesis-complete seedling regeneration process, allowing for the creation of disease-free, uniform, and high-growth-potential seed sources. The process includes the following steps: acclimatization of broodstock using seawater filtration combined with AgNPs; sterilization and sample preparation; callus induction on solid PES medium; stimulation of somatic embryogenesis using a combination of plant growth regulators NAAA-BAP; and seedling regeneration on a suitable medium. This process increases the multiplication rate, shortens seedling production time, reduces genetic degeneration, and facilitates scaling up production in laboratory and seaweed breeding facilities in Vietnam.