Systems, methods, and kits for mycotoxin cleaning

Abstract

Method for cleaning of mycotoxins includes fogging a volumetric space with anolyte hypochlorous acid having a concentration of 500 to 600 parts per million (ppm). After fogging, the volumetric space may be left undisturbed to a specified amount of time and then surfaces are wiped down. Further, the volumetric space can be re-fogged with the anolyte hypochlorous acid until humidity level reaches a specified level. Thereafter, the volumetric area can be gassed with chlorine dioxide. After completion of the chlorine dioxide treatment, a polymer coating can be applied to surfaces of the volumetric space to prevent regrowth of the mold and the production of mycotoxins.

Description

SYSTEMS, METHODS, AND KITS FOR MYCOTOXIN CLEANINGCROSS-REFERENCES TO RELATED APPLICATIONS

[0001] The present application claims priority of U.S. Provisional Application Number 63 / 464,411 filed on May 5. 2023, the entirety' of which is incorporated by reference herein for all purposes.FIELD OF DISCLOSURE

[0002] This disclosure relates generally to cleaning of mycotoxins. Particularly, systems and methods for cleaning of mycotoxins that are airborne after mold treatment within a building, enclosed spaced, or other construction site.BACKGROUND

[0003] Mold remediation solutions vary in their application. Many protocols concentrate their efforts on containing and removing visible mold and mold spores. However, even after removing the mold, harmful substances are left over. For example, substances such as invisible, odorless mycotoxins are left over in air and / or surfaces.

[0004] When mold is present in a building, it must be remediated by a professional. However, current mold remediation techniques do not address the residual contaminants like mycotoxins that the mold leaves behind. The thousands of EPA registered pesticides available are designed to kill living microorganisms like mold, but they cannot destroy the bonds of an inanimate chemical such as a mycotoxin.

[0005] Additionally , remediators can increase the toxicity of the building as they are remediating the mold because of the defense reaction of the mold. The mold has been removed and can be validated through testing, but the mycotoxins remain at higher levels.

[0006] Mold can cause allergies and breathing issues. But the most dangerous health effects are a result of the mold's defense mechanism, the mycotoxin, which can kill living organisms. Mycotoxins have been linked to numerous auto-immune diseases and are known carcinogens. When these poisonous chemicals get trapped in modem airtight buildings it can cause seriousillness and render a home uninhabitable for individuals sensitized to these chemicals. Hence, a post-mold remediation method for cleaning mycotoxins is needed to address this problem.BRIEF SUMMARY

[0007] The present disclosure provides ways of mycotoxin cleaning after a mold remediation is performed. The present disclosure provides kits and methods for getting rid of airborne and surface mycotoxins from an entire home, building or other enclosed spaces. The kits and methods herein can advantageously restore a “sick” building reducing the mycotoxin levels and thereby improving the living spaces and health of residents.

[0008] In one aspect, a method for mycotoxin cleaning is described. The method includes A method for mycotoxin cleaning post mold treatment includes fogging a volumetric space with a solution comprising anolyte hypochlorous acid having a concentration of 500 to 600 parts per million (ppm); and leaving the fogged volumetric space undisturbed for a predetermined time to allow the anolyte hypochlorous acid to entirely cover the volumetric space and to evaporate. The anolyte hypochlorous acid is an anodic type. The anolyte hypochlorous acid reacts with mycotoxins (e.g.. which can be in air and surfaces) of the volumetric space and cause weaking or break down of molecular structure of the mycotoxins. In some embodiments, the method can further include subsequent to fogging, cleaning, using disposable non-woven cloth, exposed surfaces within the volumetric space.

[0009] In some embodiments, the method can further include re-fogging the volumetric space with the solution comprising the anolyte hypochlorous acid having a concentration less than 500 ppm; and maintaining a humidity level of the volumetric space greater than 65%. In some embodiments, maintaining the humidity level above 65% can further include diluting the anolyte hypochlorous acid with water. The anolyte hypochlorous acid can be diluted to achieve a concentration of 500 ppm or less. In some embodiments, the fogging can include employing a fogging machine configured to create a mist of the solution comprising the anolyte hypochlorous acid; and turning off air conditioner and turning on HVAC fans.

[0010] In some embodiments, the method can further include after the humidity level is greater than 65% is reached, gassing the volumetric area with chlorine dioxide. The chlorine dioxide forms chlorite ion upon reacting with water. In some embodiments, the method can further include moving the gassed chloride dioxide air tow ards surfaces within the volumetric area to make maximum amount of the chlorite ion available to react with the mycotoxins. In some embodiments, the method can further include subsequent to the gassing with the chlorine dioxide, closing all windows and exterior doors or openings; and blocking sunlightfrom entering the volumetric space. In some embodiments, the method can further include subsequent to the chloride dioxide gassing, coating the surfaces within the volumetric space with a non-encapsulated polymer coating to prevent regrowth of mold and production of mycotoxins, wherein the polymer coating is an invisible water-resistant coating that is resistant to mold grow th.

[0011] In some embodiments, the surface can include moisture-exposed surfaces, previously contaminated surfaces, specified areas to protect surfaces, vents and / or conduits of HVAC. In some embodiments, the volumetric space comprises walls, ceiling, floor, crawl spaces, and / or attic.

[0012] In some embodiments, the method can further include drying and airing out the coated surfaces. In some embodiments, the method can further include verifying the coating application with UV light. In some embodiments, the method can further include activating an air mover to move the air fogged with the anolyte hypochlorous acid within the volumetric space.

[0013] In some embodiments, the method can further include determining a volume of the volumetric space; and selecting, based on the volume, a specified amount of the anolyte hypochlorous acid solution.

[0014] In another aspect, a kit for cleaning of mycotoxins is provided. The kit includes A kit for cleaning of mycotoxins including an anolyte hypochlorous acid solution having a concentration in a range from 500 to 600 ppm and reactive to mycotoxins; a chlorine dioxide bomb having a concentration in a range from 500 to 600 ppm; and a non-encapsulated polymer coating material to prevent regrowth of mold or mycotoxins. The polymer coating is an invisible w ater-base coating that is resistant to the mold. The kit can further include an instruction sheet defining a sequence and proportions of applying the anolyte hypochlorous acid solution, chlorine dioxide bomb, and the non-encapsulated polymer coating material.

[0015] The forgoing general description of the illustrative implementations and the following detailed description thereof are merely exemplary aspects of the teachings of this disclosure, and are not restrictive.BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The accompanying drawings, w hich are incorporated in and constitute a part of the specification, illustrate one or more embodiments and. together with the description, explain these embodiments. The accompanying drawings have not necessarily been drawn to scale.Any values dimensions illustrated in the accompanying graphs and figures are for illustration purposes only and can or cannot represent actual or preferred values or dimensions.

[0017] FIG. 1 is a flow chart of a method for mycotoxin cleaning, in accordance with many embodiments.

[0018] FIG. 2 is a block diagram of a system for my cotoxin cleaning, in accordance with many embodiments.

[0019] FIG. 3 a block diagram of a mycotoxin cleaning kit, in accordance with many embodiments.DETAILED DESCRIPTION

[0020] The description set forth below in connection with the appended drawings is intended as a description of various embodiments of the disclosed subject matter and is not necessarily intended to represent the only embodiment(s). In certain instances, the description includes specific details for the purpose of providing an understanding of the disclosed embodiment(s). However, it will be apparent to those skilled in the art that the disclosed embodiment(s) can be practiced without those specific details. In some instances, well-known structures and components can be shown in block diagram form in order to avoid obscuring the concepts of the disclosed subject matter. In the drawings, like reference numerals represent like parts throughout the several views.

[0021] The mold remediation industry is established with standard practices, which typically involve using chemicals and mechanical methods to eradicate mold. However, this process does not address mycotoxins — byproducts of mold that are not living organisms and are chemically resilient to conventional remediation methods. Largely unrecognized by the industry, mycotoxins remain in buildings post-remediation and can be exacerbated when mold is disturbed, potentially creating an environment more toxic than before. Energyefficient buildings compound this problem by trapping contaminated air inside. With HVAC systems circulating these toxins, and with ongoing water-damage conditions in many buildings fostering mycotoxin production, there's a significant gap in current industry standards. This oversight persists despite the introduction of new air testing methods.Consequently, a solution is urgently needed to clean mycotoxins and other contaminants from the entirety of a building's envelope after mold removal.

[0022] The present disclosure provides mycotoxin cleaning kits and methods. In many embodiments, mycotoxin cleaning involves use of at least three chemicals delivered in a particular manner and sequence to ensure satisfactory cleaning of mycotoxins. The three chemicals and delivery methods include an anolyte hypochlorous acid delivered as a fog andsprayed on individual items and surfaces, a chlorine dioxide gas treatment designed to penetrate areas that are not reachable by liquid fog, and a water-base polymer coating that prevents mycotoxin source regrowth. Accordingly, the hypochlorous acid and chlorine dioxide work together to mitigate mycotoxins, while the water-base polymer coating is moldresistant. Additional optional steps such as air filtration and purification can be included in the cleaning process depending on the applications. The present techniques and products can be configured for different types of buildings. The individual products and usage can be adjusted for applications such as small enclosed spaces or rooms of a house, a large building, an industrial shed, or other building. In addition, an untrained user or a trained professional may include additional cleaning steps. Some example steps are further discussed in detail below. The proposed methods and kits herein enable an untrained person to perform mycotoxin cleaning.

[0023] Cleaning herein can refer to removal and / or destruction of mycotoxin within an environment. In some embodiments, the cleaning can involve removal and / or destruction of more than 50%, more than 60%, more than 70%, or more than 99% of mycotoxin within an environment (e.g., an enclosed space of a home). In some embodiments, cleaning refers to active steps taken to mitigate mycotoxins within an environment.

[0024] Anolyte hypochlorous acid is an aqueous solution including HOC1" and has greatly reduced sodium content. Typical hypochlorous acids are sodium-based and have been shown ineffective in destroying the chemical structure of mycotoxin. The anolyte hypochlorous acid (HOC1) solution herein can be made by an electrochemical activation process. This process can involve taking brine w ater, sending it through specially-designed production units and adding electricity. The process triggers a reaction that causes the brine water to separate into an anolyte solution and a catholyte solution. An example reaction comprises ordinary salt brine plus electricity producing hypochlorous acid directly. The reaction can be represented as: 2NaCl + 6H2O + Electricity —> 2HOC1 + O2 + 4H2 + 2NaOH. In a typical process of producing hypochlorous acid (HOC1), chlorine gas is reacted with water, which produces Hypochlorous acid (HOC1) plus Hydrochloric Acid (HC1). In aqueous solution, the Hypochlorous Acid partially dissociates to the Hypochlorite Ion (OCT): 2HOC1 -^HOCI+ H++ OCT. The extent of the dissociation depends on the pH of the water (6.5 to 8.5). The anolyte hypochlorous acid herein is produced at a pH of between 5 and 6 making it a pow erful oxidative solution.

[0025] Anolyte Hypochlorous Acid is an organic, all-natural compound specially formulated to stand out from other manufactured HOC1 solutions. The features of this productinvolves removal of extraneous elements from the solution, ensuring no interference or competing reactions occur during the oxidation of organic compounds. Chemically manufactured HOC1 solutions typically use an acid to hold pH below a certain level to prevent the residual salts created during the manufacturing process from bonding with the HOC1 in solution. Those retain salts left in the water solution, diminish the effectiveness of the H0C1 molecule at breaking down organic chemical compounds with unsaturated bonds. Anolyte HOC1 is free from such residual salts, enhancing its oxidative capabilities.

[0026] The reaction mechanism of Anolyte HOC1 involves attacking the double bonds (unsaturation) in organic molecules, such as mycotoxins, which are highly susceptible due to their multiple unsaturation sites. This attack triggers a free radical process that not only breaks down the mycotoxin but also leaves its fragments in an oxidative state, further decomposing them back into their basic elements like carbon, hydrogen, oxygen, nitrogen, and chlorine.

[0027] Anolyte HOC1 exhibits superior oxidation potential compared to other oxidizers such as chlorine, chlorinated water, chlorine dioxide, sodium hypochlorite, hydrogen peroxide, and ozone, many of which may leave toxic residues. With an Oxidation Reduction Potential (ORP) of +700 to +900 millivolts and containing 500 to 700 ppm of free available chlorine, anolyte HOC1 operates effectively in a near-neutral pH environment. The primary' oxidizing agent in this process is the free available chlorine, available in forms such as Hypochlorous Acid (HOC1) and Hypochlorite Ions (CIO ) or as Dissolved Elemental Chlorine, ready' to react as needed.

[0028] The water-base polymer coating discussed herein can be invisible, abrasion resistant, and resistant to mold on its dry surface. The polymer coating can be applied to desired surfaces to prevent regrowth of mold thus mycotoxin release. The polymer coating is a non-encapsulating type coating. Many '‘mold-resistant’’ coatings are paint like coating with preservatives in them. These coatings create a seal when dry and do not allow moisture to pass through. Because this seal can degrade wood over time, building codes ty pically do not allow for these coatings to be used on structural wood. The polymer coating features invisible, breathable coating that allows it to be used on the structural wood of a home as well as visible areas like walls, carpets, and other areas where mold and mycotoxin protection is desired. The polymer coating comprises a polymer technology' that becomes activated by water to release the contained preservatives. As these preservatives migrate to the surface they inhibit the growth of microorganisms. In this way the water, including humidity in the air, contacting the surface of the coating activates its performance. This water can alsoactivate the spores. In some embodiments, the activation of the coating allows the antimicrobials in the coating to react with the spores and eliminates regrowth. When the water or humidity level is reduced, the coating repairs itself. On the contrary, the existing encapsulated type coating, even if they include antimicrobials therein, due to the sealing nature of the coating they do not allow the antimicrobials to migrate to the outer surface in the presence of moisture and humidity to enable a reaction for mitigating the spores and subsequent release of mycotoxins. Traditional mold resistant coatings contain preservatives similar to the way paint coatings contain pigment. Once the coating is dry, the ingredients are not released from the coating.

[0029] FIG. 1 is a flowchart of an example method 100 of mycotoxin cleaning process. The protocols in the method are applied after any active mold growth has been removed and the initial cause of water intrusion / mold growth has been remediated. The method employs anolyte hypochlorous acid to initiate the mycotoxin breakdown. In some embodiments, the method 100 involves steps 101-107 as discussed in detail below.

[0030] Step 101 can involve ULV (ultra low volume) fogging a volumetric space ith a solution comprising anolyte hypochlorous acid having a concentration of 500 to 600 parts per million (ppm). The anolyte hypochlorous acid is an anodic type free of sodium. The fogging allows the chemical to encompass many different surfaces (e.g., walls, windows, HVAC, coils, etc.) and cover a wide area. In some embodiments, a volume of the volumetric space can be determined (e.g., 1000 cubic feet. 2000 cubic feet, etc.). Based on the volume, an appropriate amount of the anolyte hypochlorous acid solution can be selected. In many embodiments, fogging can be performed using know n commercial or residential type ULV foggers.

[0031] In some embodiments, step 101 can involve ULV fogging the anolyte hypochlorous acid within the volumetric space. The volumetric space can include, but not limited to, walls, ceiling, floor, furniture, craw-1 spaces, and / or attic. The fogging can involve employing a ULV fogging machine configured to create a mist of the solution comprising the anolyte hypochlorous acid. Furthermore, the air conditioner should be turned off but the HVAC fans left on so to allow the chemicals to pass through the system.

[0032] In some embodiments, after the initial fog has settled and dried, all surfaces should be re-cleaned with the anolyte Hypochlorous Acid by spraying the surface or item and wiping with a disposable cloth or towel. For spaces with excessive dust, the surface cleaning can entail a deep and detailed micro clean and HEP A vacuum of all surfaces. Accordingly, most or all of dust and particulate matter can be removed from the surface in the space.

[0033] Step 102 can involve leaving the fogged volumetric space undisturbed for a predetermined time to allow the anolyte hypochlorous acid to cover 75% or more of the volumetric space and to evaporate. The anolyte hypochlorous acid reacts with mycotoxins in air and on surfaces of the volumetric space and causes weakening or breakdown of the molecular structure of the mycotoxins; thus initiating mycotoxin cleaning. In some embodiments, subsequent to fogging, cleaning (e.g., using a disposable cloth) of exposed surfaces can be performed within the volumetric space.

[0034] Step 103, which can be an optional step, can involve re-fogging the volumetric space with the solution comprising the anolyte hypochlorous acid having a concentration less than 500 ppm. For example, the re-fogging can be done using the anolyte hypochlorous acid having a concentration of 300 ppm. This step 103 can be performed to increase the humidity of the volumetric area. Step 103 can be performed in spaces where humidity levels are below a specified level (e.g., less than 65%). However, if an area has sufficient humidity in the air, step 103 can be omitted and the method 100 can move directly to step SI 06, discussed later.

[0035] In some embodiments, the re-fogging process can involve sealing the space with plastic sheets and painter’s tape as needed / desired. Fogging the entire space with the anolyte hypochlorous acid e.g., diluted to 300 ppm. Furthermore, indoor humidity levels can be monitored and continue to fog with hypochlorous acid until a desired a humidity level of 65% (e.g., measured by hygrometer) or higher is achieved.

[0036] Step 104 can involve determining (e.g., using a humidity sensor) whether the humidity of the volumetric space is greater than or equal to a specified level (e.g., 65%). If the humidity is lower than the specified level, step 105 can be performed. Step 105 can involve maintaining a humidity level of the volumetric space greater than 65%. This step 105 can involve re-fogging as discussed in step 103 or other ways to increase the humidity level. For example, maintaining the humidity- level above 65% can involve diluting the anolyte hypochlorous acid with water. As am example, the anolyte hypochlorous acid is diluted to achieve a concentration of 500 ppm or less.

[0037] After the specified humidity level (e.g., greater than 65%) is reached, step 106 can be performed. Step 106 can involve gassing the volumetric area with chlorine dioxide. The chlorine dioxide forms chlorite ions upon reacting with water in the air. In some embodiments, step 106 can further involve moving the gassed chloride dioxide air towards surfaces within the volumetric area to make a maximum amount of the chlorite ion available to react with the mycotoxins.

[0038] In some embodiments, step 106 may be completed at different times of days and additional steps can be performed. For example, step 106 can be completed overnight. In this case, all windows and exterior doors can be closed so that the chlorine dioxide sufficiently spreads within the volumetric area. If treating during daytime, sunlight from windows and door can be blocked prior or to immediately after fogging with chlorine dioxide. Step 106 can further involve additional, steps. For example, all plants and pets should be removed. All old air filters should be removed or discarded prior to fogging. Ensure AC is turned OFF, and run HVAC Fans ON. Step 106 can involve placing individual packets of chloring dioxide gas packets (Deodor Bomb) in all of the room of the house to ensure equal dispersion of the gas into the building. A mask such as PPE Mask (N95) may be used for application for the protection of the individual(s) delivering the chlorine dioxide. An entire volumetric space can be gassed with a calculated amount of chlorine dioxide delivered in some embodiments, via a. delivery system that uses water to dissolve sodium chlorite and a weak acid to release specified amounts of chlorine dioxide gas based on volumetric measurements. Each packet is made of compressed sponge that contains the chemicals separated by a membrane. The dry packet can be placed in the plastic tray packaging and water can be added. When water is added it dissolves the chemicals combining them and producing chlorine dioxide gas. For example, for a bedroom of about 1000 cubic ft. a 100 g chlorine dioxide bomb may be used, for a bathroom of 500 cubic feet a 50 g chlorine dioxide bomb may be used, and for a closet of 250 cubic ft a 25 g chlorine dioxide bomb may be used. Once the gassing is done, the volumetric area (e g., home) can be left empty and undisturbed for 12 hours or more. Furthermore, all solid surfaces can be dry wiped with microfiber cloth and / or vacuumed with a hepa vacuum.

[0039] Subsequent to the chloride dioxide fogging, step 107 can be performed. Step 107 can involve coating the surfaces within the volumetric space with a non-encapsulate polymer coating to prevent regrowth of mold and the subsequent release of mycotoxins. The polymer coating can be an invisible, water-resistant coating that is resistant to the grow th of mold. In some embodiments, the surfaces to be coated can include, but not be limited to moisture- exposed surfaces, previously contaminated surfaces, desired areas to protect surfaces, vents and conduits of HVAC, and / or household items like furniture, mattresses, and other goods. In some embodiments, the method 100 can further include drying and airing out the coated surfaces, without wiping coated surfaces. In some embodiments, the method 100 can further involve verifying the coating application with UV light.

[0040] In some embodiments, a pre-analysis- mycotoxin test can be performed before beginning the method 100. The test can involve: (i) identifying location(s) for airborne and surface mycotoxin sample collection e.g., living space, common area, or near HVAC intake, (ii) disturbing the air with fan or electric blower, and (iii) setting up and executing the test kit per industry guidelines.

[0041] The method 100 illustrates only an example method for cleaning of mycotoxins without limiting the scope. Other variations in steps are possible. For example, a method for cleaning of mycotoxins, the method delivering anolyte hypochlorous acid having a concentration of 500 to 600 parts per million (ppm) within a specified space. Other steps may be optional. The delivering may be performed by a fogging apparatus or other delivering means. In another example, a method for cleaning of mycotoxins can involve delivering chlorine dioxide gas having a concentration of 70 parts per million (ppm) and a dwell time of at least 12 hours within a specified space. In some embodiments, the delivering of the chlorine dioxide gas can involve gassing the specified space with chlorine dioxide, wherein the specified space has a humidity level greater than 65% and wherein the chlorine dioxide forms chlorite ion upon reacting with water. In some embodiments, the method prior to the chlorine dioxide gas, anolyte hypochlorous acid having a concentration of 500 to 600 parts per million (ppm) can be delivered within a specified space.

[0042] FIG. 2 illustrates a system 200 for cleaning of mycotoxin within a space 220. As discussed herein, the system 200 can include a mycotoxin cleaning kit 210. a fogger 221. an optional hygrometer 223, and coating applicator 225. The volumetric space 220 can be a home, a building, industrial shed, or other spaces. Depending on an amount of the volumetric space 220, the mycotoxin cleaning kit 210 can include a specified amount of anolyte hypochlorous acid 201. a specified amount of chlorine dioxide bomb 203, and a specified amount of polymer coating 205. The fogger 221 (e.g., ULV fogger) can be used to fog the volumetric space 220 with the anolyte hypochlorous acid 201. In some environments, a hygrometer 223 may be used to determine a humidity of the space 220. Determining that the humidity is greater than a specified amount (e.g., greater than 65%) can be beneficial before deploying the chlorine dioxide bomb 203 within the space 220. In some embodiments, after treating the space 220 with the anolyte hypochlorous acid 201 and the chlorine dioxide 203, the polymer coating 205 can be applied, via an airless sprayer (using a 411 tip) or a typical garden sprayer with a fine mist application, to surfaces within the volumetric space 220 to prevent regrowth of mold and production of mycotoxins.

[0043] FIG. 3 illustrates a cleaning kit for mycotoxin cleaning 300 that can be provided to a user along with instructions such as in steps of the method 100. The kit allows the user to perform, after mold remediation process is complete, removal of residual contaminants, including but not limited to, mycotoxins. The cleaning kit can include chemicals or materials in specified proportions and instructions discussed in the method above. For example, the kit300 can include an anolyte hypochlorous acid solution 301, a chlorine dioxide bomb 303, and a non-encapsulated polymer coating material 305. The anolyte hypochlorous acid solution301 can have a concentration in a range from 500 to 600 ppm and reactive to mycotoxins. The chlorine dioxide bomb 303 can have a concentration in a range from 500 to 600 ppm. The non-encapsulated polymer coating material 305 is configured to prevent regrowth of mold or mycotoxins. The polymer coating is an invisible water-resistant coating that is resistant to mold growth. The kit 300 can further include an instruction sheet defining steps of the method 100. For example, the instructions can include a sequence and proportions of applying the anolyte hypochlorous acid solution 301, chlorine dioxide bomb 303, and the non-encapsulated polymer coating material 305.

[0044] Below are non-limiting examples of proportions and costs of chemicals used in the mycotoxin cleaning process.A. Usage example for fogging with Anolyte Hypochlorous Acid for 1000 ft2space @ 8 ft height• Total cubic feet area - 8000 ft3• Normal fogger coverage area = 1 gallon per 5000 ft3B. Usage example for surface cleaning with anolyte hypochlorous acid• Normal Coverage area = 1 gallon per 300 ft2.C. Usage example for gassing with Deodor Bomb of chlorine dioxide for 1000 ft2space @ 8 ft height (e.g., living area)• Total cubic feet area - 8000 ft3• Normal gassing coverage area = lx 100g packet = 1000 ft3D. Usage example for gassing with Deodor Bomb of Chlorine dioxide for 1000 ft2space @ 5 ft height (e.g., non-living area)• Total cubic feet area - 5000 fit3• Normal gassing coverage area = lx 100g Pro packet = 2000 fit3Usage example for protecting with Endurance Coating Pro™ for 1000 fit2crawl space @ 5 ft crawl height• Flat Surface - 1000 ft2• Joist Area - 1248 ft2(12 in joist at 16 in on center, 23.7 joist per 31.6 ft, coat both side and top of joist)• Crawl area - 631 ft2(5 ft height crawl area at all four sides)• 10% waste - 288 ft2• Total area - 3168 ft2• Normal Coverage area = 1 gallon per 600 ft2.

[0045] A collection of exemplary embodiments, including at least some explicitly enumerated as “Examples’" providing additional description of a variety of example types in accordance with the concepts described herein are provided below. These examples are not meant to be mutually exclusive, exhaustive, or restrictive; and the invention is not limited to these example examples but rather encompasses all possible modifications and variations within the scope of the issued claims and their equivalents.

[0046] Example 1. A method for mycotoxin cleaning includes: fogging a volumetric space with a solution comprising anolyte hypochlorous acid having a concentration of 500 to 600 parts per million (ppm), wherein the anolyte hypochlorous acid is an anodic type free of sodium; and leaving the fogged volumetric space undisturbed for a pre-determined time to allow the anolyte hypochlorous acid to cover 75% or more of the volumetric space and to evaporate. The anolyte hypochlorous acid reacts with mycotoxins in the volumetric space and cause weaking or break down of molecular structure of the mycotoxins.

[0047] Example 2. The method of any of the preceding or subsequent examples or combination of examples, further comprising: subsequent to fogging, cleaning, using disposable non-woven cloth, exposed surfaces within the volumetric space.

[0048] Example 3. The method of any of the preceding or subsequent examples or combination of examples, further comprising: re-fogging the volumetric space with the solution comprising the anolyte hypochlorous acid having a concentration less than 500 ppm; and maintaining a humidity level of the volumetric space greater than 65%.

[0049] Example 4. The method of any of the preceding or subsequent examples or combination of examples, wherein maintaining the humidity' level above 65% comprises: diluting the anolyte hypochlorous acid with water, wherein the anolyte hypochlorous acid is diluted to achieve a concentration of 500 ppm or less.

[0050] Example 5. The method of any of the preceding or subsequent examples or combination of examples, further comprising: after the humidity level is greater than 65% isreached, gassing the volumetric area with chlorine dioxide, wherein the chlorine dioxide forms chlorite ion upon reacting with water.

[0051] Example 6. The method of any of the preceding or subsequent examples or combination of examples, further comprising: moving the gassed chloride dioxide air towards surfaces within the volumetric area to make maximum amount of the chlorite ion available to react with the mycotoxins.

[0052] Example 7. The method of any of the preceding or subsequent examples or combination of examples, further comprising: subsequent to the gassing with the chlorine dioxide, closing all windows and exterior doors or openings; and blocking sunlight from entering the volumetric space.

[0053] Example 8. The method of any of the preceding or subsequent examples or combination of examples, further comprising: subsequent to the chloride dioxide gassing, coating the surfaces within the volumetric space with a non-encapsulated polymer coating to prevent regrowth of mold and production of mycotoxins, wherein the polymer coating is an invisible water-resistant coating that is resistant to mold growth.

[0054] Example 9. The method of any of the preceding or subsequent examples or combination of examples, wherein the surfaces comprise: moisture-exposed surfaces, previously contaminated surfaces, and desired areas to protect surfaces.

[0055] Example 10. The method of any of the preceding or subsequent examples or combination of examples, wherein the surfaces comprise: vents and conduits of HVAC.

[0056] Example 1 1 . The method of any of the preceding or subsequent examples or combination of examples, further comprising drying and airing out the coated surfaces.

[0057] Example 12. The method of any of the preceding or subsequent examples or combination of examples, further comprising verifying the coating application with UV light.

[0058] Example 13. The method of any of the preceding or subsequent examples or combination of examples, further comprising: activating an air mover to move the air fogged with the anolyte hypochlorous acid within the volumetric space.

[0059] Example 14. The method of any of the preceding or subsequent examples or combination of examples, wherein the volumetric space comprises walls, ceiling, floor, crawl spaces, and / or attic.

[0060] Example 15. The method of any of the preceding or subsequent examples or combination of examples, wherein the fogging comprises: employing a fogging machine configured to create a mist of the solution comprising the anolyte hypochlorous acid; and turning off air conditioner and turning on HVAC fans.

[0061] Example 16. The method of any of the preceding or subsequent examples or combination of examples, further comprising: determining a volume of the volumetric space: and selecting, based on the volume, a specified amount of the anolyte hypochlorous acid solution.

[0062] Example 17. A kit for cleaning of mycotoxins, the kit comprising: an anolyte hypochlorous acid solution having a concentration in a range from 500 to 600 ppm and reactive to mycotoxins: and a chlorine dioxide bomb having a concentration in a range from 500 to 600 ppm.

[0063] Example 18. The kit of any of the preceding or subsequent examples or combination of examples, further comprising a non-encapsulated polymer coating material to prevent regrowth of mold or mycotoxins, wherein the polymer coating is an invisible water-resistant coating that is resistant to the mold.

[0064] Example 19. The kit of any of the preceding or subsequent examples or combination of examples, further comprising an instruction sheet defining a sequence and proportions of applying the anolyte hypochlorous acid solution, chlorine dioxide bomb, and the nonencapsulated polymer coating material.

[0065] Example 20. A method for cleaning of mycotoxins, the method comprising: delivering anolyte hypochlorous acid having a concentration of 500 to 600 parts per million (ppm) within a specified space.

[0066] Example 21. A method for cleaning of mycotoxins, the method comprising: delivering chlorine dioxide gas having a concentration of 70 parts per million (ppm) and a dwell time of at least 12 hours within a specified space.

[0067] Example 22. The method of any of the preceding or subsequent examples or combination of examples, wherein delivering the chlorine dioxide gas comprises gassing the specified space with chlorine dioxide, wherein the specified space has a humidity level greater than 65% and wherein the chlorine dioxide forms chlorite ion upon reacting with water.

[0068] Example 23. The method of any of the preceding or subsequent examples or combination of examples, further comprising: delivering, prior to the chlorine dioxide gas, anolyte hypochlorous acid having a concentration of 500 to 600 parts per million (ppm) within a specified space.

[0069] Based on the disclosure and teachings provided herein, a person of ordinary skill in the art will appreciate other ways and / or methods to implement the various embodiments.The specification and drawings are, accordingly, to be regarded in an illustrative rather than arestrictive sense. It will, however, be evident that various modifications and changes may be made thereunto without departing from the broader spirit and scope of the disclosure as set forth in the claims.

[0070] Other variations are within the spirit of the present disclosure. Thus, while the disclosed techniques are susceptible to various modifications and alternative constructions, certain illustrated embodiments thereof are shown in the drawings and have been described above in detail. It should be understood, however, that there is no intention to limit the disclosure to the specific form or forms disclosed, but on the contrary, the intention is to cover all modifications, alternative constructions, and equivalents falling within the spirit and scope of the disclosure, as defined in the appended claims.

[0071] The use of the terms “a” and “an” and "‘the7’ and similar referents in the context of describing the disclosed embodiments (especially in the context of the following claims) are to be constmed to cover both the singular and the plural, unless otherwise indicated herein or clearly contradicted by context. The terms “comprising,” “having.” “including,” and “containing” are to be construed as open-ended terms (i.e., meaning “including, but not limited to,”) unless otherwise noted. The term “connected” is to be construed as partly or wholly contained within, attached to, or joined together, even if there is something intervening. Recitation of ranges of values herein are merely intended to serve as a shorthand method of referring individually to each separate value falling within the range, unless otherwise indicated herein and each separate value is incorporated into the specification as if it were individually recited herein. All methods described herein can be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by context. The use of any and all examples, or exemplary language (e.g., “such as”) provided herein, is intended merely to better illuminate embodiments of the disclosure and does not pose a limitation on the scope of the disclosure unless otherwise claimed. No language in the specification should be constmed as indicating any non-claimed element as essential to the practice of the disclosure.

[0072] Disjunctive language such as the phrase “at least one of X, Y, or Z,” unless specifically stated otherwise, is intended to be understood within the context as used in general to present that an item, term, etc., may be either X, Y, or Z, or any combination thereof (e.g., X, Y, and / or Z). Thus, such disjunctive language is not generally intended to, and should not, imply that certain embodiments require at least one of X, at least one of Y, or at least one of Z to each be present.

Claims

CLAIMSWHAT IS CLAIMED IS:

1. A method for mycotoxin cleaning comprising: fogging a volumetric space with a solution comprising anolyte hypochlorous acid having a concentration of 500 to 600 parts per million (ppm), wherein the anolyte hypochlorous acid is an anodic type free of sodium; and leaving the fogged volumetric space undisturbed for a pre-determined time to allow the anolyte hypochlorous acid to cover 75% or more of the volumetric space and to evaporate, wherein the anolyte hypochlorous acid reacts with mycotoxins in the volumetric space and cause weaking or break down of molecular structure of the mycotoxins.

2. The method of claim 1, further comprising: subsequent to fogging, cleaning, using disposable non-woven cloth, exposed surfaces within the volumetric space.

3. The method of claim 1, further comprising: re-fogging the volumetric space with the solution comprising the anolyte hypochlorous acid having a concentration less than 500 ppm; and maintaining a humidity level of the volumetric space greater than 65%.

4. The method of claim 3, wherein maintaining the humidity level above 65% comprises: diluting the anolyte hypochlorous acid with water, wherein the anolyte hypochlorous acid is diluted to achieve a concentration of 500 ppm or less.

5. The method of claim 3, further comprising: after the humidity level is greater than 65% is reached, gassing the volumetric area with chlorine dioxide, wherein the chlorine dioxide forms chlorite ion upon reacting with water.

6. The method of claim 5, further comprising: moving the gassed chloride dioxide air towards surfaces within the volumetric area to make maximum amount of the chlorite ion available to react with the mycotoxins.

7. The method of claim 5, further comprising: subsequent to the gassing with the chlorine dioxide, closing all windows and exterior doors or openings; and blocking sunlight from entering the volumetric space.

8. The method of claim 5, further comprising: subsequent to the chloride dioxide gassing, coating the surfaces within the volumetric space with a non-encapsulated polymer coating to prevent regrowth of mold and production of mycotoxins, wherein the polymer coating is an invisible water-resistant coating that is resistant to mold growth.

9. The method of claim 8, wherein the surfaces comprise: moisture-exposed surfaces, previously contaminated surfaces, and desired areas to protect surfaces.

10. The method of claim 8, wherein the surfaces comprise: vents and conduits of HVAC.

11. The method of claim 8, further comprising drying and airing out the coated surfaces.

12. The method of claim 8, further comprising verifying the coating application with UV light.

13. The method of claim 1, further comprising: activating an air mover to move the air fogged with the anolyte hypochlorous acid within the volumetric space.

14. The method of claim 1, wherein the volumetric space comprises walls, ceiling, floor, crawl spaces, and / or attic.

15. The method of claim 1, wherein the fogging comprises: employing a fogging machine configured to create a mist of the solution comprising the anolyte hypochlorous acid; andturning off air conditioner and turning on HVAC fans.

16. The method of claim 1, further comprising: determining a volume of the volumetric space; and selecting, based on the volume, a specified amount of the anolyte hypochlorous acid solution.

17. A kit for cleaning of mycotoxins, the kit comprising: an anolyte hypochlorous acid solution having a concentration in a range from 500 to 600 ppm and reactive to mycotoxins; and a chlorine dioxide bomb having a concentration in a range from 500 to 600 ppm.

18. The kit of claim 17, further comprising a non-encapsulated polymer coating material to prevent regrowth of mold or mycotoxins, wherein the polymer coating is an invisible water- resistant coating that is resistant to the mold.

19. The kit of claim 17, further comprising an instruction sheet defining a sequence and proportions of applying the anolyte hypochlorous acid solution, chlorine dioxide bomb, and the non-encapsulated polymer coating material.

20. A method for cleaning of mycotoxins, the method comprising: delivering anolyte hypochlorous acid having a concentration of 500 to 600 parts per million (ppm) within a specified space.

21. A method for cleaning of mycotoxins, the method comprising: delivering chlorine dioxide gas having a concentration of 70 parts per million (ppm) and a dwell time of at least 12 hours within a specified space.

22. The method of claim 21, wherein delivering the chlorine dioxide gas comprises gassing the specified space with chlorine dioxide, wherein the specified space has a humidity level greater than 65% and wherein the chlorine dioxide forms chlorite ion upon reacting with water.

23. The method of claim 21, further comprising:delivering, prior to the chlorine dioxide gas, anolyte hypochlorous acid having a concentration of 500 to 600 parts per million (ppm) within a specified space.

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