A system and method for propagating and improving the adaptability of native shrub seedlings suitable for high-altitude and arid regions.

Abstract

This invention belongs to the field of native shrub seedling propagation technology, and discloses a system and method for propagating and improving the adaptability of native shrub seedlings suitable for high-altitude and arid regions. The system includes a data processing platform, acquisition equipment, and control equipment. The data processing platform establishes habitat and germplasm archives corresponding to the same germplasm batch and sequentially completes germplasm dormancy treatment, staged seedling management, root culture, mycotoxin-seedling symbiosis, stress substrate integration, and planting feedback. The method generates qualified germplasm records and germination initiation records based on germplasm status and dormancy type. Seedling stage status records serve as the basis for initiating the next stage. Root culture, mycotoxin inoculation, stress pre-adaptation, and substrate transition are performed based on the habitat information of the target restoration plot. After planting, survival rate, greening time, new shoot growth, and overwintering survival rate are recorded in the habitat and germplasm archive to correct parameters for subsequent batches.

Description

Technical Field

[0001] This invention belongs to the field of native shrub seedling propagation technology, specifically a native shrub seedling propagation and adaptability enhancement system and method suitable for high-altitude and arid regions. Background Technology

[0002] High-altitude and arid regions are typically characterized by prolonged periods of low temperatures, large diurnal temperature variations, insufficient rainfall, low soil moisture content, strong ultraviolet radiation, and low soil nutrient levels. Native shrubs, having long adapted to such environments, are often used for vegetation restoration in degraded grasslands, desertified lands, mining sites, and slope areas. The current propagation of native shrub seedlings generally includes steps such as germplasm collection, germplasm screening, dormancy breaking, nursery seedling cultivation, hardening-off, and field planting. Some seedling cultivation processes also combine container seedling cultivation, water-controlled hardening-off, or rhizosphere inoculation with growth-promoting bacteria to improve the adaptability of seedlings after transplanting.

[0003] In actual breeding processes, there are significant differences in germplasm quality, dormancy type, source habitat, and target recovery site conditions. Within the same batch of germplasm, there may be shriveled grains, insect-eaten grains, damaged grains, and germplasm with varying degrees of dormancy release. Soil moisture content, nutrient levels, low temperature intensity, and UV conditions also vary across different target sites. Existing treatment methods often follow fixed experiences to separately screen, soak, stratify, raise seedlings, inoculate, and harden off the seedlings. There is a lack of continuous status records for each step, which are formed around the same batch of germplasm. Germplasm screening results are difficult to transmit to dormancy release, root development status is difficult to transmit to inoculum inoculation, post-inoculation recovery status is difficult to transmit to stress acclimatization, and stress recovery status is also difficult to transmit to substrate transition and planting management.

[0004] Because of the lack of continuous connection between the above-mentioned stages, the seedling condition formed in the nursery stage is prone to mismatch with the actual low temperature, water shortage, ultraviolet radiation, diurnal temperature range and soil conditions of the target restoration plot.

[0005] If seedlings enter the inoculation or stress stage before their root systems have developed a structure suitable for water absorption and transplanting, the inoculation effect is likely to be unstable. If the stress acclimatization is not compatible with the target plot environment, seedlings are prone to prolonged seedling recovery period, slow greening, root water loss, and fluctuations in overwintering survival rate after transplanting. If the survival rate, greening time, new shoot growth, and overwintering survival rate after transplanting are only kept as acceptance data and are not used for subsequent germplasm batch screening, dormancy release, segmentation judgment, inoculation, and substrate transition parameter adjustment, subsequent seedling cultivation in similar plots will still need to rely on repeated corrections based on experience, making it difficult to form a stable and reusable propagation process. Summary of the Invention

[0006] The purpose of this invention is to provide a system and method for the propagation and adaptability enhancement of native shrub seedlings suitable for high-altitude and arid regions, so as to solve the problems mentioned in the background art.

[0007] To achieve the above objectives, the present invention provides the following technical solution: a native shrub seedling propagation and adaptability enhancement system suitable for high-altitude and arid regions, which solves the problem of lack of continuous connection between germplasm screening, dormancy release, root culture, inoculum inoculation, stress acclimatization, substrate transition and field planting in the existing native shrub seedling process.

[0008] Existing seedling cultivation methods typically involve separate processes for germplasm screening, seedling cultivation, hardening-off, and transplanting. There is a lack of state transfer between these processes based on the same germplasm batch, source area, and target restoration plot. This can easily lead to uneven germplasm germination, incompatibility between seedling roots and planting soil, unstable timing of microbial inoculation, difficulty in controlling the intensity of stress acclimatization, and large fluctuations in survival rate and overwintering survival rate after planting.

[0009] To solve the above problems, the present invention adopts the following technical solution.

[0010] This system includes a data processing platform, acquisition equipment, and control equipment. The acquisition equipment is connected to the data processing platform and is used to collect information on the germplasm source, germplasm status, dormancy type, source habitat, target restoration site habitat, rhizosphere soil sample source, seedling root status, seedling stage status, and post-planting growth of the target native shrubs. The control equipment is connected to the data processing platform and is used to receive control commands output by the data processing platform and to adjust the seedling environment, stress culture conditions, and substrate transition conditions. Through the above settings, a continuous data correspondence is formed between the germplasm source, seedling process, rhizosphere soil samples, and target restoration site. A closed-loop control relationship is also formed, in which the habitat and germplasm archive outputs control parameters, each processing unit performs control, and the planting feedback unit writes back and corrects parameters.

[0011] The data processing platform includes a habitat and germplasm archive unit, a germplasm dormancy treatment unit, a staged seedling management unit, a root culture unit, a mycotoxin seedling symbiosis unit, a stress substrate integration unit, and a planting feedback unit.

[0012] The habitat provenance archive unit binds germplasm source information, target native shrub dormancy type information, provenance habitat information, target restoration site habitat information, and rhizosphere soil sample source information for the same germplasm batch, forming a habitat provenance archive. This archive is not merely a static database for storing data; rather, it is configured as a set of control parameters that can be accessed by various functional units. It includes a basic data area, a stage status area, and a control parameter area. The basic data area stores provenance, target restoration site, and rhizosphere soil sample source data. The stage status area records germplasm screening and dormancy resolution data. In addition to recording the status of stage transition, root culture, seedling inoculation, stress recovery, substrate transition, and post-planting, the control parameter area outputs quality grading conditions, dormancy release treatment methods, transition judgment conditions, inoculation judgment conditions, combined stress sequences, and substrate transition ratios to each treatment unit based on the above data. Through this archive, the same germplasm batch always corresponds to the same germplasm source, the same target recovery plot, and the corresponding rhizosphere soil sample source throughout the entire propagation process. Each unit can output control instructions based on the same control benchmark, reducing the deviation of seedling parameters caused by the mixing of data from different batches.

[0013] The germplasm dormancy treatment unit generates qualified germplasm records and germination initiation records based on germplasm status information and target native shrub dormancy type information. Germplasm status information includes the integrity of the outline, surface color difference, insect holes, cracks, mold spots, and plumpness in germplasm images or measured germplasm information. Based on the above information, the germplasm dormancy treatment unit classifies germplasm into qualified germplasm, shriveled grains, insect-infested grains, damaged grains, and moldy grains, and removes shriveled grains, insect-infested grains, damaged grains, and moldy grains. For the retained qualified germplasm, the germplasm dormancy treatment unit determines the corresponding treatment items among mechanical bark breaking, warm water soaking, low temperature stratification, variable temperature stratification, and plant growth regulator soaking, in conjunction with the target native shrub dormancy type information, and determines the treatment time, treatment temperature, stratification period, or soaking concentration. Through this treatment method, the germplasm screening results directly participate in the determination of dormancy release methods, so that the germplasm entering the germination and seedling establishment stage has a more consistent quality basis and germination initiation conditions.

[0014] Based on qualified germplasm records and germination initiation records, the staged seedling management unit sequentially introduces qualified germplasm into the germination and seedling establishment stage, root system configuration culture stage, mycotoxin-seedling symbiotic culture stage, stress pre-acclimatization culture stage, substrate transition culture stage, and open-field hardening-off stage. At the end of each stage, a seedling stage status record is generated, and the record of the previous stage serves as the basis for initiating the next stage. The germination and seedling establishment stage records the whitening rate and germination uniformity; the root system configuration culture stage records the taproot length, lateral root number, and root-to-shoot ratio; the mycotoxin-seedling symbiotic culture stage records the continued growth status of lateral roots after inoculation and the degree of leaf wilting; the stress pre-acclimatization culture stage records the recovery culture time, new shoot growth status, and stem uprightness; and the substrate transition culture stage records the degree of leaf wilting after substrate transition, the recovery growth status of new shoots, and stem uprightness. By controlling the initiation of subsequent stages through these stage status records, the premature entry of seedlings into the next stage before the root system has reached the inoculation threshold, before the mycotoxin has recovered after inoculation, or before the stress treatment has healed can be prevented.

[0015] The root culture unit generates a root system architecture status record based on the habitat information of the target restoration site and the root status information of the seedlings. The habitat information relevant to root culture in the target restoration site includes soil moisture content, soil organic matter content, total nitrogen content, available phosphorus content, and available potassium content. The seedling root status information includes taproot length, lateral root number, and root-shoot ratio. The root culture unit reads the substrate moisture content target, aeration requirements, nutrient supply, and root-promoting treatments corresponding to the target restoration site from the control parameter area of ​​the habitat provenance file. The root culture unit directly reads the soil moisture content data of the target restoration plot in the habitat germplasm archive and uses it as the target benchmark for the control equipment to adjust the moisture content of the seedling substrate. This allows the seedlings to form a root system that is adapted to the planting environment of high-altitude and arid regions during the nursery stage. This step is not simply to promote rapid growth of seedlings, but to make the root culture correspond to the water and nutrient conditions of the target restoration plot, providing a root system foundation for subsequent inoculum inoculation, stress pre-adaptation and field planting.

[0016] The microbial seedling symbiosis unit obtains candidate functional strains from rhizosphere soil samples corresponding to the rhizosphere soil sample source information based on root system architecture records. Functional strains for inoculation are then selected from these candidate strains. These functional strains are determined based on low-temperature culture records, drought stress culture records, root-promoting control records, and rhizosphere recovery records. The selected functional strains are then prepared as a compound microbial agent. The inoculation time and number of inoculations are determined based on the number of lateral roots, root-to-shoot ratio, seedling substrate moisture content, and leaf wilting level. This ensures that the compound microbial agent inoculation occurs after lateral root formation and before stress pre-adaptation culture. Through this setup, inoculation is no longer based solely on a fixed seedling age, but rather on the seedling root system status and leaf wilting level. This helps reduce insufficient adaptation caused by premature inoculation before the root system has formed an inoculation foundation or by inoculation after the seedling has entered the stress stage.

[0017] The stress substrate transition unit forms a combined stress sequence based on the root recovery record after inoculation and the habitat information of the target restoration plot. The combined stress sequence includes low temperature stress and water control stress. According to the UV intensity and diurnal temperature range of the target restoration plot, UV stress, diurnal temperature range stress, or a combination of both are configured. After executing the combined stress sequence, the system generates a stress recovery record and determines the substrate transition ratio based on the record. Through this transition method, stress pre-adaptation no longer adopts a single hardening-off method, but corresponds to the low temperature, water, UV intensity, and diurnal temperature range conditions of the target restoration plot, so that the seedlings have undergone a pre-adaptation process that matches the target environment before entering the substrate transition.

[0018] During the substrate transition stage, the stress substrate integration unit gradually mixes native soil or transition substrate into the seedling substrate. The transition substrate is configured according to the particle composition, pH, electrical conductivity, water retention capacity and nutrient level of the target restoration plot soil. By adjusting the substrate composition step by step, the seedlings gradually come into contact with soil conditions similar to the target restoration plot before leaving the nursery, reducing the problems that may occur when directly transferring from the nursery substrate to the field soil, such as root water loss, excessive changes in nutrient supply and prolonged seedling recovery time.

[0019] After the seedlings have been hardened off in the open field, the planting feedback unit plants them in the target restoration plot while maintaining the integrity of the root ball. The post-planting growth information is written into the habitat germplasm archive, including survival rate, greening time, new shoot growth, and overwintering survival rate. Based on the above information, the planting feedback unit generates parameter correction results and writes them into the control parameter area of ​​the habitat germplasm archive. These results are used to correct the quality grading conditions, dormancy release treatment methods, segment transition judgment conditions, inoculation judgment conditions, combined stress sequences, and substrate transition ratios for subsequent germplasm batches. Through this feedback mechanism, the field performance of the previous batch of seedlings can have a reverse effect on the seedling parameters of the next batch, forming a closed loop in the breeding and adaptability improvement process consisting of archive parameter output, stage status writing, planting result feedback, and parameter correction.

[0020] This invention also provides a method for the propagation and adaptation enhancement of native shrub seedlings suitable for high-altitude and arid regions. This method uses the same batch of germplasm as the treatment object and continuously connects germplasm source, dormancy release, staged seedling cultivation, root culture, mycotoxin symbiosis, stress pre-adaptation, substrate transition, open-field hardening-off, and planting feedback. The state record formed in the previous stage serves as the basis for starting the next stage, reducing the problem of inconsistent parameters caused by independent handling of each step based on experience in traditional seedling cultivation.

[0021] First, germplasm source information, dormancy type information, provenance habitat information, target restoration site habitat information, and rhizosphere soil sample source information for the same germplasm batch of the target native shrub were collected and recorded. A habitat and provenance file corresponding to each germplasm batch was established. Germplasm source information included the germplasm batch, collection location, and collection time; provenance habitat information included the provenance elevation, low-temperature data, and precipitation data; and target restoration site habitat information included low-temperature data, precipitation data, and soil moisture content. Water volume, soil organic matter content, total nitrogen content, available phosphorus content, available potassium content, pH, electrical conductivity, ultraviolet intensity, and diurnal temperature range; rhizosphere soil sample source information includes rhizosphere soil sample number, rhizosphere soil sample collection location, and corresponding target native shrub name. The above information comes from germplasm collection records, target native shrub dormancy characteristic records, field survey data of the seed source area, environmental monitoring data of the target restoration plot, and rhizosphere soil sample collection records, so that subsequent germplasm treatment, seedling transfer, inoculant inoculation, and stress acclimatization have a unified data source.

[0022] During the germplasm treatment stage, germplasm status information from the same batch of germplasm is collected. Based on the germplasm status information and the dormancy type information of the target native shrub, qualified germplasm records and germination initiation records are formed. The germplasm status information comes from germplasm images or measured germplasm information, including outline integrity, surface color difference, insect holes, cracks, mold spots, and plumpness. Based on the above information, the germplasm is divided into qualified germplasm, shriveled grains, insect-damaged grains, broken grains, and moldy grains. Shriveled grains, insect-damaged grains, broken grains, and moldy grains are removed. For the retained qualified germplasm, the treatment items among mechanical bark breaking, warm water soaking, low temperature stratification, variable temperature stratification, and plant growth regulator soaking are determined based on the dormancy type information of the target native shrub and the plumpness of the qualified germplasm. The treatment time, treatment temperature, stratification period, or soaking concentration of the corresponding treatment items are also determined. Through this treatment process, the germplasm entering the germination and seedling establishment stage has both quality foundation and dormancy-breaking foundation, reducing the problem of uneven germination caused by the mixing of shriveled grains, insect-damaged grains, and dormant germplasm.

[0023] After dormancy is broken, qualified germplasm is introduced into the seedling system, which then sequentially enters the germination and seedling establishment stage, root system configuration culture stage, mycotoxin-seedling symbiotic culture stage, stress pre-adaptation culture stage, substrate transition culture stage, and open-field hardening stage. At the end of each stage, a seedling stage status record is formed, and the seedling stage status record of the previous stage is used as the basis for starting the next stage.

[0024] At the end of the germination and seedling establishment stage, the white sprouting rate and germination uniformity are recorded; at the end of the root system structure culture stage, the taproot length, lateral root number, and root-to-shoot ratio are recorded; at the end of the mycotoxin-seedling symbiotic culture stage, the continued growth status of lateral roots after inoculation and the degree of leaf wilting are recorded; at the end of the stress pre-adaptation culture stage, the degree of leaf wilting, recovery culture time, new shoot growth status, and seedling stem uprightness are recorded; at the end of the substrate transition culture stage, the degree of leaf wilting after substrate transition, new shoot recovery growth status, and seedling stem uprightness are recorded. Through the above stage status records, the next stage will no longer be started according to a fixed number of days, but will be started according to the actual state of the seedlings formed in the previous stage, reducing the situation where the root system has not yet formed, has not yet recovered after inoculation, or has not yet recovered after stress, and the seedlings enter the next stage prematurely.

[0025] During the root system architecture culture stage, root system architecture status records are generated based on the habitat information of the target restoration plot and the root system status information of the seedlings. Specifically, based on the soil moisture content, soil organic matter content, total nitrogen content, available phosphorus content, and available potassium content of the target restoration plot, the moisture content of the seedling substrate, substrate aeration, nutrient supply, and root-promoting treatment cycle are adjusted. During the root system culture process, the length of the taproot, the number of lateral roots, and the root-to-shoot ratio are recorded so that the seedling root system status can serve as the basis for entering the mycotoxin-seedling symbiotic culture stage. This process ensures that the root system culture corresponds to the water and nutrient conditions of the target restoration plot, avoiding the situation where only the aboveground growth of the seedlings is pursued while the adaptability of the root system to the cold and arid soil environment is ignored.

[0026] During the mycotococulture stage, candidate functional strains were obtained from rhizosphere soil samples corresponding to the root system architecture records. From these candidate strains, the functional strains used for inoculation were determined based on low-temperature culture records, drought stress culture records, root-promoting control records, and rhizosphere recovery records. Low-temperature culture records reflected the growth of candidate strains under low-temperature conditions; drought stress culture records reflected their growth under water stress; root-promoting control records reflected differences in taproot length, lateral root number, or root-to-shoot ratio between inoculated and uninoculated treatments; and rhizosphere recovery records indicated whether the functional strains could be re-obtained from the rhizosphere of the target native shrub after inoculation. After determining the functional strains for inoculation, they were prepared into a compound mycotocopheric agent. The inoculation time and number of inoculations were determined based on the number of lateral roots, root-to-shoot ratio, seedling substrate moisture content, and leaf wilting level, ensuring that inoculation occurred after lateral root formation and before stress pre-acclimatization culture. This treatment synchronized inoculation with the seedling root development status, reducing the instability of inoculation effects before the root system had formed a suitable inoculation foundation.

[0027] During the stress pre-adaptation culture stage, a combined stress sequence was formed based on the root recovery record after inoculation and the habitat information of the target restoration plot. The combined stress sequence included low temperature stress and water control stress, and one or two stress items, such as ultraviolet stress and diurnal temperature variation stress, were added according to the ultraviolet intensity and diurnal temperature variation of the target restoration plot. After the combined stress sequence was executed, a stress recovery record was formed, which included the leaf wilting level, recovery culture time, new shoot growth status, and seedling stem erection status. This record was used to determine whether the seedlings had entered the substrate transition culture stage. Through this treatment, the seedlings underwent a pre-adaptation process corresponding to the low temperature, water, ultraviolet intensity, and diurnal temperature variation of the target restoration plot before being transplanted, reducing the difficulty of seedling recovery caused by sudden environmental changes when directly transferred to the field environment.

[0028] During the substrate transition culture stage, the substrate transition ratio is determined based on stress recovery records. The native soil or transition substrate is then gradually mixed into the seedling substrate according to this ratio. The transition substrate is configured according to the particle composition, pH, electrical conductivity, water retention capacity, and nutrient level of the target restoration plot soil. Each stage of substrate transition is judged based on the degree of leaf wilting, the recovery of new shoot growth, and the uprightness of the seedling stems after substrate transition to determine whether to proceed to the next ratio. By gradually introducing native soil or transition substrate, the seedlings gradually come into contact with soil conditions similar to the target restoration plot during the nursery stage, reducing the problems of root water loss, sudden changes in nutrient supply, and prolonged recovery time that may occur when directly transferring from the seedling substrate to the field soil.

[0029] After the substrate transition culture is completed, the seedlings are transferred to the outdoor hardening stage. During the outdoor hardening stage, the seedlings are gradually exposed to natural light, natural day-night temperature difference and natural wind environment. The wilting level of leaves, the recovery of new shoot growth and the uprightness of seedling stems are recorded after the outdoor hardening. This stage is used to confirm that the seedlings can still maintain a basic growth state after leaving the controlled seedling environment, providing a transitional basis for subsequent field planting.

[0030] After the seedlings have been hardened off in the open field, they are transplanted to the target restoration plot while maintaining the integrity of the root ball. Post-transplant growth information is collected and written into the habitat germplasm archive. This post-transplant growth information includes survival rate, greening time, new shoot growth, and overwintering survival rate. This information is used to correct the quality grading conditions, dormancy release treatment methods, segment transition judgment conditions, inoculation judgment conditions, combined stress sequences, and substrate transition ratios for subsequent germplasm batches. Through this feedback process, the field performance of the previous batch of seedlings can reversely correct the seedling parameters of the next batch, allowing the propagation process to be continuously adjusted according to the actual transplanting results of the target restoration plot.

[0031] Using the above methods, germplasm screening results are used to determine the basis for dormancy release; dormancy release results are used to determine the starting point for germination and seedling establishment; root system architecture status is used to determine the timing of inoculum inoculation; post-inoculation root recovery records are used to determine the initiation of stress pre-adaptation; stress recovery records are used to determine the substrate transition ratio; seedling status after substrate transition is used to determine the basis for hardening off in the open field; and post-planting growth information is used to correct parameters for subsequent germplasm batches. A continuous state transfer relationship is formed between each stage, which can reduce the parameter mismatch problem caused by the disconnect between different links in the traditional seedling cultivation process, making it more suitable for the propagation, vegetation restoration, and ecological restoration of native shrub seedlings in cold and arid regions.

[0032] The beneficial effects of this invention are as follows: 1. This invention unifies the germplasm source information, target native shrub dormancy type information, germplasm source habitat information, target restoration plot habitat information, and rhizosphere soil sample source information of the same germplasm batch into the habitat germplasm archive. This provides a common data basis for germplasm screening, dormancy release, seedling transfer, root culture, inoculum inoculation, stress pre-adaptation, substrate transition, and planting feedback. This method avoids the batch information breakage problem caused by separate recording, judgment, and adjustment at each stage in the existing seedling process. It ensures that the same germplasm batch maintains clear source, target plot, and treatment records throughout the entire propagation process. This facilitates the determination of subsequent seedling parameters based on germplasm quality, dormancy type, and target restoration plot conditions, reducing problems such as uneven germination, mismatched treatment intensity, and difficulty in tracing seedling parameters caused by mixing data from different batches or biased experience judgment.

[0033] 2. This invention uses the seedling stage status records formed in the previous stage as the basis for starting the next stage, thus creating a continuous connection between germination and seedling establishment, root system configuration culture, mycotoxin-seedling symbiotic culture, stress pre-adaptation culture, substrate transition culture, and open-field hardening-off. Specifically, the white sprouting rate and germination uniformity are used to determine whether to enter root system configuration culture; the taproot length, lateral root number, and root-to-shoot ratio are used to determine whether to enter mycotoxin-seedling symbiotic culture; the continued growth status of lateral roots after inoculation and the degree of leaf wilting are used to determine whether to enter stress pre-adaptation culture; and the recovery culture time after stress, the growth status of new shoots, and the uprightness of seedling stems are used to determine whether to enter substrate transition culture. This process reduces the possibility of prematurely entering the next stage when the seedling root system has not yet formed, has not recovered after inoculation, or is unstable after stress treatment. It transforms the seedling cultivation process from being driven by a fixed number of days to being driven by the actual state of the seedlings, improving the coordination and stability between stages.

[0034] 3. This invention, through the continuous coordination of root cultivation, mycorrhizal symbiosis, stress pre-adaptation, substrate transition, and planting feedback, enables seedlings to gradually adapt to the soil moisture, nutrient levels, low temperature, water control, UV intensity, and diurnal temperature range of the target cold and arid recovery site before transplanting. During the root cultivation stage, the substrate moisture content, aeration, nutrient supply, and root-promoting treatment cycle are adjusted according to the soil moisture and nutrient conditions of the target site. During the mycorrhizal symbiosis stage, the timing of compound mycorrhizal inoculation is determined based on the root condition. During the stress pre-adaptation stage, a combined stress sequence is formed based on the environment of the target site. During the substrate transition stage, the transition substrate is configured according to the soil particle composition, pH, electrical conductivity, water retention capacity, and nutrient levels of the target site. After planting, the survival rate, greening time, new shoot growth, and overwintering survival rate are recorded in the habitat germplasm archive to correct parameters for subsequent batches, thereby improving the reusability and planting adaptability of the subsequent propagation process for similar sites. Attached Figure Description

[0035] Figure 1 This is a flowchart illustrating the propagation and adaptability enhancement of native shrub seedlings applicable to high-altitude and arid regions according to the present invention. Figure 2 This is a flowchart of the seedling cultivation process in this invention. Figure 3 This is a flowchart of the strain-strain symbiosis and stress substrate integrator of the present invention. Detailed Implementation

[0036] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0037] like Figures 1 to 3 As shown, this embodiment of the invention provides a system for the propagation and adaptability enhancement of native shrub seedlings suitable for high-altitude and arid regions. The system includes a data processing platform, a data acquisition device, and a control device. The data acquisition device is connected to the data processing platform and is used to collect information on the germplasm source, germplasm status, dormancy type, habitat of the seed source, habitat of the target restoration plot, rhizosphere soil sample source, seedling root status, seedling stage status, and post-planting growth of the target native shrub. The control device is connected to the data processing platform and is used to adjust the seedling environment, stress culture conditions, and substrate transition conditions.

[0038] The data processing platform uses computers, servers, industrial control computers, or nursery management terminals with the capabilities of data storage, data retrieval, status judgment, and control command output. The data processing platform is equipped with habitat and germplasm archive units, germplasm dormancy treatment units, staged seedling management units, root culture units, mycotoxin seedling symbiosis units, stress substrate incorporation units, and planting feedback units. The above units are jointly implemented through databases, data processing programs, on-site control programs, and manual confirmation records. The records formed in the previous treatment stage serve as the basis for starting the next treatment stage, and each unit can call the control parameter area in the habitat and germplasm archive. After the parameters in the habitat and germplasm archive are called, they form the control equipment's commands for adjusting water, temperature, light, ultraviolet, diurnal temperature range, nutrient supply, and substrate ratio. Therefore, the archive plays the role of dynamic control benchmark in the system.

[0039] The data collection equipment includes germplasm image acquisition equipment, germplasm weighing equipment, germplasm appearance inspection equipment, meteorological data acquisition equipment, soil moisture acquisition equipment, soil sampling tools, soil physicochemical index testing equipment, seedling image acquisition equipment, root system observation equipment, and post-planting monitoring equipment. Germplasm image acquisition equipment is used to obtain germplasm surface conditions; meteorological data acquisition equipment is used to obtain low temperature data, precipitation data, ultraviolet intensity, and diurnal temperature range at the source site and target restoration plot; soil sampling tools and soil physicochemical index testing equipment are used to obtain soil moisture content, soil organic matter content, total nitrogen content, available phosphorus content, available potassium content, pH, electrical conductivity, particle size distribution, water retention capacity, and nutrient levels; root system observation equipment is used to obtain taproot length, lateral root number, and root-to-shoot ratio; post-planting monitoring equipment is used to obtain survival rate, greening time, new shoot growth, and overwintering survival rate. The above data collection processes can be automated or a combination of on-site testing and manual data entry.

[0040] The control equipment includes irrigation equipment, drainage equipment, ventilation equipment, shading equipment, supplemental lighting equipment, temperature control equipment, ultraviolet irradiation equipment, diurnal temperature range control equipment, substrate mixing equipment, and nutrient supply equipment. Irrigation equipment is used to regulate the moisture content of the seedling substrate; drainage and ventilation equipment are used to improve the moisture content and aeration of the substrate; nutrient supply equipment is used to regulate the nutrient supply in the seedling substrate; temperature control equipment, ultraviolet irradiation equipment, and diurnal temperature range control equipment are used to implement low temperature stress, water control stress, ultraviolet stress, and diurnal temperature range stress; substrate mixing equipment is used to mix native soil or transitional substrate into the seedling substrate according to the substrate transition ratio.

[0041] In practical applications, the target native shrubs and target restoration plots are first identified. The target native shrubs are shrub species that are naturally distributed in high-altitude and arid regions or have long been adapted to the local environment. When collecting germplasm, germplasm from the same collection area, the same collection time period, and the same target species is numbered as a germplasm batch. Germplasm source information includes germplasm batch, germplasm collection location, and collection time. The germplasm collection location is determined by the on-site collection record, location record, or collection site number, and the collection time is determined by the collection record. Through the germplasm batch number, subsequent germplasm screening, dormancy breaking, seedling cultivation, inoculation, stress, substrate transition, and planting feedback all correspond to germplasm from the same source.

[0042] Information on the dormancy type of target native shrubs is derived from existing cultivation data, germplasm biology data, seedling experience records, or small-scale pre-test results for the target species. For target species for which there is no clear data, the dormancy type is determined through small-scale water absorption tests, seed coat permeability observation, short-term germination pre-tests, low-temperature stratification pre-tests, or variable-temperature stratification pre-tests. If the germplasm has difficulty absorbing water and the seed coat is relatively hard, it is recorded as hard-grained germplasm; if the germplasm still cannot germinate normally after absorbing water, it is recorded as physiologically dormant germplasm; if both seed coat permeability impairment and insufficient embryo after-ripening exist simultaneously, it is recorded as compound dormancy germplasm. The above judgment results are recorded in the habitat germplasm archive as the basis for dormancy release treatment.

[0043] The habitat information of the seed origin includes the altitude of the seed origin, low temperature data of the seed origin, and precipitation data of the seed origin. The above information was obtained through field surveys of the seed source area, local meteorological data, forestry and grassland monitoring data, or long-term observation data. Habitat information for the target restoration site includes low temperature data, precipitation data, soil moisture content, soil organic matter content, total nitrogen content, available phosphorus content, available potassium content, pH, electrical conductivity, ultraviolet intensity, and diurnal temperature range. This information was obtained through field sensors, manual sampling and testing, meteorological station records, and soil testing reports. Soil moisture content was obtained through soil moisture sensors or sampling and drying methods. Soil organic matter content, total nitrogen content, available phosphorus content, and available potassium content were obtained through soil sample testing. pH and electrical conductivity were obtained through soil extract testing. Ultraviolet intensity and diurnal temperature range were obtained through field observations of the target restoration site or regional meteorological monitoring data. Rhizosphere soil sample source information includes rhizosphere soil sample number, rhizosphere soil sample collection location, and the corresponding target native shrub name. Rhizosphere soil samples were collected from the soil near the rhizosphere of the target native shrub, and the collection location, collection date, and corresponding plant were recorded at the time of collection.

[0044] The habitat and germplasm archive unit binds germplasm source information, target native shrub dormancy type information, germplasm source habitat information, target restoration plot habitat information, and rhizosphere soil sample source information for the same germplasm batch, forming a habitat and germplasm archive. The habitat and germplasm archive is set up with a basic data area, a stage status area, and a control parameter area. The basic data area records the germplasm batch, germplasm source habitat, target restoration plot habitat, and rhizosphere soil sample source. The stage status area records the subsequently formed qualified germplasm records, germination initiation records, seedling stage status records, root system architecture status records, post-inoculation root recovery records, stress recovery records, substrate transition records, and post-planting growth information. The control parameter area records and outputs quality grading conditions, dormancy release treatment methods, segment transition judgment conditions, inoculation judgment conditions, combined stress sequences, and substrate transition ratios. The parameters in the control parameter area are not manually viewed and set separately, but are directly read by the germplasm dormancy treatment unit, stage seedling management unit, root culture unit, mycotoxin symbiosis unit, and stress substrate connection unit, thus forming the control basis for the control equipment.

[0045] The germplasm dormancy treatment unit generates qualified germplasm records and germination initiation records based on germplasm status information and dormancy type information of the target native shrubs. Germplasm status information is obtained through germplasm images or measured germplasm information. Germplasm images are used to identify outline integrity, surface color difference, insect holes, cracks, and mold spots. Measured germplasm information includes single grain weight, length and width dimensions, thickness, or water absorption status, which is used to assist in judging the plumpness. Based on the above information, the germplasm dormancy treatment unit classifies germplasm into qualified germplasm, shriveled grains, insect-damaged grains, broken grains, and moldy grains, and removes shriveled grains, insect-damaged grains, broken grains, and moldy grains. Qualified germplasm records include the quantity of qualified germplasm, the quantity of removed germplasm, the type of removal, and the corresponding germplasm batch.

[0046] For qualified germplasm, the germplasm dormancy treatment unit determines the dormancy-breaking treatment items based on the dormancy type information of the target native shrub and the plumpness of the qualified germplasm. For hard-shelled germplasm, mechanical desquamation or warm water soaking is used to improve seed coat permeability. For physiologically dormant germplasm, low-temperature stratification, variable-temperature stratification, or soaking with plant growth regulators is used to promote embryo maturation or improve germination ability. For compound dormant germplasm, treatment to improve seed coat permeability is performed first, followed by treatment to promote embryo maturation. The treatment time, treatment temperature, stratification period, or soaking concentration is determined based on the results of small-scale pre-tests of the target species, existing seedling data, or nursery technical regulations, and recorded in the germination initiation record. The germination initiation record includes the dormancy-breaking treatment item, treatment time, treatment temperature, stratification period, soaking concentration, treatment end time, and the number of qualified germplasm entering the germination and seedling establishment stage. Through this record, the germination and seedling establishment stage can clearly identify the qualified germplasm that has completed screening and dormancy breaking.

[0047] Based on records of qualified germplasm and germination initiation, the phased seedling management unit sequentially guides qualified germplasm into the germination and seedling establishment stage, root system architecture culture stage, mycotoxin-seedling symbiotic culture stage, stress pre-acclimatization culture stage, substrate transition culture stage, and open-field hardening-off stage. At the end of each stage, a seedling stage status record is generated, and the initiation of the next stage is based on the seedling stage status record of the previous stage. This method differs from seedling cultivation methods that only follow a fixed number of days, establishing a state transfer relationship between each stage.

[0048] During the germination and seedling establishment stage, qualified germplasm that has undergone dormancy-breaking treatment is placed in a suitable germination substrate, and appropriate moisture and aeration conditions are maintained. At the end of this stage, the emergence rate and germination uniformity are recorded. The emergence rate reflects whether the germplasm has entered the germination state, and the germination uniformity reflects whether the germination of the same batch of germplasm is concentrated. When the emergence rate and germination uniformity meet the preset transition requirements, the root system configuration culture stage is entered. If the preset transition requirements are not met, the germination observation time is extended, and the dormancy-breaking treatment method and germination performance of this batch are recorded in the habitat germplasm file for subsequent batches to be corrected.

[0049] During the root system architecture cultivation stage, the root system cultivation unit reads the soil moisture content, soil organic matter content, total nitrogen content, available phosphorus content, and available potassium content of the target restoration plot from the habitat provenance archive basic data area, and reads the seedling root system status information from the stage status area to form a root system architecture status record. The seedling root system status information includes taproot length, lateral root number, and root-to-shoot ratio. Taproot length is obtained through root sampling observation, transparent root observation containers, root scanning, or manual measurement; lateral root number is statistically analyzed through root images or sampling observation; root-to-shoot ratio is determined based on root biomass and aboveground biomass measurements, or calculated based on root and aboveground measurement data allowed by the nursery. The root system cultivation unit controls and regulates irrigation, drainage, ventilation, and nutrient supply according to the substrate moisture content target, substrate aeration requirements, nutrient supply, and root promotion treatment cycle output from the control parameter area. If the soil moisture content of the target restoration plot is low, the taproot is induced to grow downward by moderately controlling water and improving the aeration of the substrate; if the nutrient level of the target restoration plot is low, the probability of seedling etiolation under high nutrient conditions is reduced, and lateral root formation is promoted by root-promoting treatment. The root system configuration status record includes taproot length, lateral root number and root-to-shoot ratio, which serves as the basis for entering the myco-species symbiotic culture stage.

[0050] During the mycorrhizal culture stage, the mycorrhizal unit obtains candidate functional strains from rhizosphere soil samples corresponding to the source information of the rhizosphere soil samples based on the root system architecture records. These candidate functional strains are obtained through microbial isolation and culture. During isolation and culture, the rhizosphere soil sample number, culture conditions, candidate strain number, and isolation source are recorded. Subsequently, based on low-temperature culture records, drought stress culture records, root-promoting control records, and rhizosphere recovery records, the functional strains used for inoculation are determined from the candidate functional strains. Low-temperature culture records reflect the growth of candidate functional strains under low-temperature conditions; drought stress culture records reflect the growth of candidate functional strains under water stress conditions; root-promoting control records reflect the differences in taproot length, lateral root number, or root-to-shoot ratio between inoculated and uninoculated treatments; and rhizosphere recovery records involve re-isolating strains from the seedling rhizosphere soil samples after inoculation and confirming whether the corresponding functional strains can be obtained again based on colony morphology, physiological and biochemical characteristics, or sequence alignment results. The functional strains determined through the above records correspond to the rhizosphere environment of the target native shrubs and the stress conditions of the target restoration plot.

[0051] After identifying the functional strains for inoculation, they are prepared into compound microbial agents. The compound microbial agents can be liquid, solid carrier, or mixed with seedling substrate. The specific form is determined according to the nursery inoculation conditions. The inoculation time and number of inoculations of the compound microbial agents are determined by the number of lateral roots, root-to-shoot ratio, seedling substrate moisture content, and leaf wilting level in the microbial-seedling symbiosis unit. The compound microbial agent inoculation occurs after lateral roots have formed and before stress pre-adaptation culture. This setting can avoid premature inoculation when the seedling roots have not yet formed an effective contact area, and can also avoid unstable colonization of the strains due to inoculation after the seedlings have entered strong stress.

[0052] A root recovery record is generated after inoculation, including inoculation time, number of inoculations, continued growth status of lateral roots after inoculation, and leaf wilting level. This record serves as the basis for entering the stress pre-adaptation culture stage.

[0053] During the stress pre-adaptation culture stage, the stress substrate integrator unit forms a combined stress sequence based on the root recovery record after inoculation and the habitat information of the target recovery plot. The combined stress sequence includes low temperature stress and water control stress. According to the UV intensity and diurnal temperature difference of the target recovery plot, UV stress, diurnal temperature difference stress or a combination of the two are configured. The combined stress sequence records the stress type, low temperature target temperature, target value of substrate moisture content, UV irradiation conditions, diurnal temperature difference conditions, stress duration and recovery culture time.

[0054] In one embodiment, low-temperature stress is achieved through a gradient treatment from -5°C to 5°C, water-controlled stress maintains the moisture content of the seedling substrate at 30% to 50%, and ultraviolet stress is performed at an intensity of 15 W / m². 2 The specific implementation intensity shall be determined within the above range based on the measured data of the target restoration plot and the recovery status of the seedlings.

[0055] The target low temperature, target substrate moisture content, ultraviolet irradiation conditions, diurnal temperature range, stress duration, and recovery culture time were determined based on the measured data of the target recovery plot, leaf wilting level, seedling stem uprightness, new shoot growth status, and the recovery culture time of the previous stage.

[0056] After executing the combined stress sequence, a stress recovery record is generated, which includes the leaf wilting level, recovery culture time, new shoot growth status, and seedling stem uprightness. When the leaf wilting level decreases, the seedling stem remains upright, and the new shoot resumes growth after recovery culture, the seedling enters the substrate transition culture stage. If the seedling leaf wilting level does not decrease or the seedling stem does not recover its uprightness, the stress intensity of the next stage is reduced or the recovery culture time is extended, and this information is recorded in the habitat and germplasm archive.

[0057] During the substrate transition culture stage, the stress substrate connection unit determines the substrate transition ratio based on the stress recovery record, and mixes the native soil or transition substrate into the seedling substrate step by step according to the substrate transition ratio. The native soil comes from the target recovery plot or a sampling area with soil type similar to the target recovery plot. The transition substrate is configured according to the particle composition, pH, electrical conductivity, water retention capacity and nutrient level of the target recovery plot soil.

[0058] In one implementation, the proportion of target soil or transitional substrate in the seedling substrate is adjusted sequentially to 20%, 40%, 60%, and 100%. Each proportion is maintained until the seedling shoots resume growth, the wilting level of the leaves decreases, and the seedling stems remain upright before moving to the next proportion.

[0059] The particle composition is adjusted by the ratio of sand, silt, and clay; pH ​​is adjusted by acidic or alkaline soil conditioner; electrical conductivity is adjusted by controlling the soluble salt content; water retention capacity is adjusted by adjusting the content of organic matter, humus, vermiculite, or perlite; and nutrient levels are adjusted based on the test results of total nitrogen, available phosphorus, and available potassium.

[0060] Each substrate transition stage generates a substrate transition record, which includes the proportion of native soil, the proportion of transition substrate, the duration, the level of leaf wilting, the recovery of new shoot growth, and the state of seedling stem uprightness. Once the seedling shoots recover and the level of leaf wilting decreases at the current proportion, the next proportion is entered. Through this step-by-step transition, the seedling root system can gradually adapt to the soil physicochemical conditions similar to those of the target restoration plot.

[0061] During the outdoor hardening-off stage, seedlings that have undergone substrate transition culture are transferred to an environment with natural light, natural day-night temperature difference, and natural wind for hardening-off. Outdoor hardening-off is not direct transplanting, but rather observing the state of seedlings after leaving the controlled seedling environment in a nursery or near the target environment. During outdoor hardening-off, the level of leaf wilting, the recovery of new shoot growth, and the uprightness of the seedling stem are recorded. After the seedlings have maintained a reduced level of leaf wilting, a recovery of new shoot growth, and an uprightness of the seedling stem in the outdoor environment, they are transplanted to the target restoration plot while maintaining the integrity of the root ball. Maintaining the integrity of the root ball is achieved by transplanting with substrate, transplanting with containers, or transplanting with minimal disturbance from the container.

[0062] After transplanting, the transplanting feedback unit collects post-transplanting growth information and writes it into the stage status area of ​​the habitat germplasm archive. Post-transplanting growth information includes survival rate, greening time, new shoot growth, and overwintering survival rate. Survival rate reflects the survival status of plants after transplanting; greening time reflects the time from seedling establishment to recovery of growth; new shoot growth reflects the growth recovery ability after transplanting; and overwintering survival rate reflects the survival status of seedlings after the low-temperature season. Based on the above information, the transplanting feedback unit generates parameter correction results and writes them into the control parameter area. These results are used to correct the quality grading conditions, dormancy release treatment methods, segment transition judgment conditions, inoculation judgment conditions, combined stress sequences, and substrate transition ratios for subsequent germplasm batches.

[0063] When the survival rate of a batch of seedlings is low and the recovery time is prolonged, increase the transition requirements for root system configuration records in subsequent batches, extend the recovery culture time after stress, or reduce the incremental increase of substrate transition ratio; when the overwintering survival rate is low, adjust the stage arrangement of low temperature stress in the combined stress sequence, and extend the recovery culture observation time after low temperature stress in subsequent batches.

[0064] In this embodiment, the habitat germplasm archive runs through the entire process of germplasm treatment, staged seedling raising, root culture, mycotoxin symbiosis, stress pre-adaptation, substrate transition, open-field hardening-off, and planting feedback. Germplasm screening results are used to determine the basis for dormancy release, dormancy release results are used to determine the starting point for germination and seedling establishment, root system architecture status is used to determine the timing of mycotoxin inoculation, root recovery records after inoculation are used to determine the initiation of stress pre-adaptation, stress recovery records are used to determine the substrate transition ratio, substrate transition records are used to determine the basis for open-field hardening-off, and growth information after planting is used to correct the parameters of subsequent germplasm batches. Unlike separate germplasm sorting, separate mycotoxin inoculation, separate stress resistance hardening-off, or separate container planting, this embodiment outputs control benchmarks to each treatment unit through the control parameter area, and writes back the field performance as parameter correction results through the planting feedback unit, so that the system forms a continuous closed-loop control structure.

[0065] Comparison and verification examples To illustrate the impact of the aforementioned closed-loop control structure on the adaptability of native shrubs to planting in arid and cold regions, a comparative verification example was conducted. Using the same germplasm source, the same target restoration plot, and the same planting season, a native shrub germplasm was divided into four groups, with each group receiving the same number of seedlings. Control group 1 employed traditional segmented seedling cultivation, including conventional seed treatment, seedbed cultivation, and conventional hardening-off. Control group 2 only underwent mycotocin-seedling symbiosis treatment, with the remaining steps performed according to traditional methods. Control group 3 only underwent stress pre-adaptation treatment, with the remaining steps performed according to traditional methods. The present invention employs a complete system with continuous integration of habitat germplasm archives, root system architecture culture, mycotocin-seedling symbiosis, combined stress, substrate transition, and planting feedback.

[0066] In this comparative verification example, the combined stress of the present invention group adopted a low temperature gradient of -5°C to 5°C, water control treatment with a matrix moisture content of 30% to 50%, and 15W / m 2 Under ultraviolet irradiation conditions, during the substrate transition stage, the seedling substrate was replaced stepwise according to the target site soil ratios of 20%, 40%, 60%, and 100%. After transplanting, the survival rate one month after transplanting, the survival rate after overwintering, the time to regrowth, root activity, and annual new root growth were recorded for each group. Root activity was expressed as a relative value, with the measured value of control group 1 as 100%. The experimental results are as follows.

[0067] Control group 1 used traditional segmented seedling cultivation. The survival rate was 45% one month after transplanting, 36% after overwintering, and the greening time was 25 days. The relative value of root vitality was 100%, and the annual growth of new roots was 4.8 cm.

[0068] Control group 2 only used the bacterial-seedling symbiotic treatment. The survival rate after one month of planting was 58%, the survival rate after overwintering was 45%, the greening time was 21 days, the relative root vigor was 118%, and the annual growth of new roots was 6.1 cm.

[0069] Control group 3 only received stress preconditioning treatment. The survival rate after one month of planting was 63%, the survival rate after overwintering was 51%, the greening time was 19 days, the relative root vigor was 126%, and the annual growth of new roots was 6.8 cm.

[0070] The invention group adopts a complete closed-loop system. The survival rate after one month of planting is 85%, the survival rate after overwintering is 74%, the greening time is 15 days, the relative value of root vitality is 156%, and the annual growth of new roots is 10.2 cm.

[0071] The results above show that while increasing the co-culture treatment or stress pre-adaptation treatment alone improves the survival rate and overwintering survival rate, the improvement is limited. In contrast, this invention group uses habitat and germplasm archives as a dynamic control parameter set, ensuring continuous connection between root culture, inoculum inoculation, combined stress, substrate transition, and planting feedback. This resulted in a one-month survival rate increase from 45% to 85%, an overwintering survival rate increase from 36% to 74%, and a greening time shortened from 25 days to 15 days. Simultaneously, root vigor and annual new root growth were significantly improved. These results demonstrate that the state transfer and feedback correction between units are not simply parallel and superimposed, but rather work together to improve the root recovery capacity and overwintering adaptability of seedlings when transitioning from the nursery environment to the high-altitude, arid target recovery plot.

[0072] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0073] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A system for propagating and improving the adaptability of native shrub seedlings suitable for high-altitude, cold, and arid regions, characterized in that, It includes a data processing platform, a data acquisition device connected to the data processing platform, and a control device connected to the data processing platform. The collection device is used to collect information on the germplasm source, germplasm status, dormancy type, habitat of the source site, habitat of the target restoration plot, rhizosphere soil source, seedling root status, seedling stage status, and growth after planting of the target native shrub. The control device is used to receive control commands output by the data processing platform and to adjust the seedling environment, stress culture conditions and substrate transition conditions. The data processing platform includes a habitat and germplasm archive unit, a germplasm dormancy treatment unit, a stage seedling management unit, a root culture unit, a mycotoxin seedling symbiosis unit, a stress substrate connection unit, and a planting feedback unit. The habitat germplasm archive unit is used to bind germplasm source information, target native shrub dormancy type information, germplasm source habitat information, target restoration plot habitat information, and rhizosphere soil sample source information of the same germplasm batch, forming a habitat germplasm archive corresponding to the same germplasm batch. The habitat germplasm archive includes a basic data area, a stage status area, and a control parameter area. The basic data area is used to store the germplasm source, target restoration plot, and rhizosphere soil sample source data corresponding to the same germplasm batch. The stage status area is used to write qualified germplasm records, germination initiation records, seedling stage status records, root system configuration status records, root recovery records after inoculation, stress recovery records, substrate transition records, and post-planting growth information. The control parameter area is used to output quality grading conditions, dormancy release treatment methods, transition judgment conditions, inoculation judgment conditions, combined stress sequences, and substrate transition ratios to the germplasm dormancy treatment unit, stage seedling management unit, root culture unit, mycotoxin-seedling symbiosis unit, and stress substrate connection unit. The germplasm dormancy treatment unit is used to read the quality grading conditions and dormancy release treatment methods in the control parameter area, and to form qualified germplasm records and germination initiation records based on germplasm status information and dormancy type information of the target native shrub; The stage seedling management unit is used to read the transition judgment conditions in the control parameter area, so that qualified germplasm enters the germination and seedling establishment, root system configuration culture, myco-seedling symbiotic culture, stress pre-adaptation culture, substrate transition culture and open hardening in sequence, and the seedling stage status record of the previous stage is used as the basis for starting the next stage. The root culture unit is used to read the target restoration plot habitat information in the basic data area and the seedling root status information in the stage status area, form a root configuration status record, and output adjustment instructions for substrate moisture content, substrate aeration, nutrient supply and root promotion treatment cycle to the control equipment. The microbial seedling symbiosis unit is used to obtain candidate functional strains from the rhizosphere soil sample corresponding to the rhizosphere soil sample source information based on the root system configuration state record and the inoculation judgment conditions in the control parameter area, and to determine the functional strains for inoculation from the candidate functional strains. The functional strains for inoculation are prepared into a compound microbial agent and then inoculated into the seedling rhizosphere to form a root recovery record after inoculation. The stress matrix transition unit is used to output adjustment commands for low temperature, water control, ultraviolet light, diurnal temperature difference and matrix ratio to the control device based on the root recovery record after inoculation, the habitat information of the target recovery plot, and the combined stress sequence and matrix transition ratio in the control parameter area. After executing the combined stress sequence, it generates a stress recovery record and determines the matrix transition ratio for the next stage based on the stress recovery record and performs matrix transition. The planting feedback unit is connected to the habitat germplasm archive unit for writing post-planting growth information into the stage status area. It generates parameter correction results based on survival rate, greening time, new shoot growth, and overwintering survival rate. The parameter correction results are written into the control parameter area, so that the control parameter area outputs corrected quality grading conditions, dormancy release treatment methods, segmentation judgment conditions, inoculation judgment conditions, combined stress sequences, and substrate transition ratios to subsequent germplasm batches.

2. The system for propagating and improving the adaptability of native shrub seedlings suitable for high-altitude and arid regions according to claim 1, characterized in that: The germplasm source information includes germplasm batch, germplasm collection location, and collection time; the germplasm source habitat information includes germplasm source altitude, germplasm source low temperature data, and germplasm source precipitation data; the target restoration plot habitat information includes target restoration plot low temperature data, precipitation data, soil moisture content, soil organic matter content, total nitrogen content, available phosphorus content, available potassium content, pH, electrical conductivity, ultraviolet intensity, and diurnal temperature range; the rhizosphere soil sample source information includes rhizosphere soil sample number, rhizosphere soil sample collection location, and the corresponding target native shrub name; the post-planting growth information includes survival rate, greening time, new shoot growth, and overwintering survival rate.

3. The system for propagating and improving the adaptability of native shrub seedlings suitable for high-altitude and arid regions according to claim 2, characterized in that: The germplasm dormancy treatment unit includes a germplasm grading section and a dormancy matching section. The germplasm grading section is used to classify germplasm into qualified germplasm, shriveled grains, insect-bored grains, cracks, mold spots, and moldy grains based on the outline integrity, surface color difference, insect holes, cracks, mold spots, and plumpness in germplasm images or measured germplasm information. The dormancy matching section is used to determine the treatment items among mechanical bark breaking, warm water soaking, low temperature stratification, variable temperature stratification, and plant growth regulator soaking based on the dormancy type information of the target native shrub and the plumpness of qualified germplasm, and to determine the corresponding treatment time, treatment temperature, stratification period, or soaking concentration for each treatment item.

4. The native shrub seedling propagation and adaptability enhancement system suitable for high-altitude and arid regions according to claim 3, characterized in that: The staged seedling management unit includes a stage transition determination unit. At the end of the germination and seedling establishment stage, the stage transition determination unit uses the white sprouting rate and germination uniformity as the criteria for entering the root system configuration culture stage. At the end of the root system configuration culture stage, the main root length, the number of lateral roots, and the root-to-shoot ratio are used as the criteria for entering the mycotoxin-seedling symbiotic culture stage. At the end of the mycotoxin-seedling symbiotic culture stage, the continued growth status of lateral roots after inoculation and the degree of leaf wilting are used as the criteria for entering the stress pre-adaptation culture stage. At the end of the stress pre-adaptation culture stage, the recovery culture time, the growth status of new shoots, and the uprightness of seedling stems are used as the criteria for entering the substrate transition culture stage. At the end of the substrate transition culture stage, the degree of leaf wilting, the recovery growth status of new shoots, and the uprightness of seedling stems under open-air conditions are used as the criteria for entering the open-air hardening-off stage.

5. The native shrub seedling propagation and adaptability enhancement system suitable for high-altitude and arid regions according to claim 4, characterized in that: The root culture unit controls and regulates substrate moisture content, substrate aeration, nutrient supply, and root-promoting treatment cycle based on soil moisture content, soil organic matter content, total nitrogen content, available phosphorus content, and available potassium content of the target restoration plot. The microbial-seedling symbiosis unit determines the functional strains for inoculation based on low-temperature culture records, drought stress culture records, root-promoting control records, and rhizosphere recovery records of candidate functional strains, and determines the inoculation time and number of inoculations of the compound microbial agent based on the number of lateral roots, root-shoot ratio, seedling substrate moisture content, and leaf wilting level. The stress substrate connection unit forms a combined stress sequence including low-temperature stress and water control stress, and configures ultraviolet stress, diurnal temperature variation stress, or a combination of both based on the ultraviolet intensity and diurnal temperature range of the target restoration plot. The transition substrate is configured according to the particle composition, pH, electrical conductivity, water retention capacity, and nutrient level of the soil in the target restoration plot.

6. A method for propagating and improving the adaptability of native shrub seedlings suitable for high-altitude, cold, and arid regions, characterized in that: The system for propagating and improving the adaptability of native shrub seedlings suitable for high-altitude and arid regions, as described in any one of claims 1 to 5, comprises the following steps: Collect and record germplasm source information, dormancy type information, source habitat information, target restoration plot habitat information, and rhizosphere soil sample source information of the same germplasm batch of target native shrubs. Establish a habitat germplasm source file corresponding to the germplasm batch and configure the habitat germplasm source file to include a basic data area, a stage status area, and a control parameter area. Based on the germplasm source information, dormancy type information of target native shrubs, habitat information of germplasm source sites, habitat information of target restoration plots, and rhizosphere soil sample source information in the basic data area, initial control parameters are generated and written into the control parameter area. The initial control parameters include quality grading conditions, dormancy release treatment methods, segmentation judgment conditions, inoculation judgment conditions, combined stress sequences, and substrate transition ratio. Germplasm status information of the same germplasm batch is collected, and the germplasm dormancy treatment unit reads the quality grading conditions and dormancy release treatment method in the control parameter area. Based on the germplasm status information and the dormancy type information of the target native shrub, qualified germplasm records and germination start records are formed and written into the stage status area. Based on the records of qualified germplasm and germination initiation, qualified germplasm is introduced into the seedling system, so that qualified germplasm enters the germination and seedling establishment stage, root system configuration culture stage, myco-seedling symbiotic culture stage, stress pre-adaptation culture stage, substrate transition culture stage and open hardening stage in sequence. At the end of each stage, a seedling stage status record is generated and written into the stage status area. The stage seedling management unit reads the transition judgment conditions in the control parameter area and uses the seedling stage status record of the previous stage as the basis for starting the next stage. During the root system configuration culture stage, the root system culture unit reads the target restoration plot habitat information in the basic data area and the seedling root system status information in the stage status area, forms a root system configuration status record, and outputs adjustment instructions to the control equipment for substrate moisture content, substrate aeration, nutrient supply and root promotion treatment cycle. During the symbiotic culture stage, the symbiotic unit obtains candidate functional strains from the rhizosphere soil sample corresponding to the rhizosphere soil sample source information based on the root system configuration state record and the inoculation judgment conditions in the control parameter area. It then determines the functional strains for inoculation from the candidate functional strains, prepares the functional strains for inoculation into a compound microbial agent, and inoculates it into the seedling rhizosphere to form a root recovery record after inoculation. During the stress pre-adaptation culture stage, the stress matrix integrator unit outputs adjustment commands for low temperature, water control, ultraviolet light and diurnal temperature difference to the control device based on the root recovery record after inoculation, the habitat information of the target recovery plot and the combined stress sequence in the control parameter area, and generates a stress recovery record after executing the combined stress sequence. During the substrate transition culture stage, the stress substrate connection unit outputs a substrate ratio adjustment command to the control device based on the stress recovery record and the substrate transition ratio in the control parameter area, and forms a substrate transition record after the substrate transition. After hardening off in the open air, the seedlings are transplanted to the target restoration plot while maintaining the integrity of the root ball. Post-transplant growth information is collected and written into the stage status area. The transplant feedback unit generates parameter correction results based on the post-transplant growth information and writes the parameter correction results into the control parameter area for subsequent germplasm batches to access the corrected quality grading conditions, dormancy release treatment methods, segment transition judgment conditions, inoculation judgment conditions, combined stress sequences, and substrate transition ratios.

7. The method for propagating and improving the adaptability of native shrub seedlings suitable for high-altitude and arid regions according to claim 6, characterized in that: The germplasm source information includes germplasm batch, germplasm collection location, and collection time; the germplasm source habitat information includes germplasm source altitude, germplasm source low temperature data, and germplasm source precipitation data; the target restoration plot habitat information includes target restoration plot low temperature data, precipitation data, soil moisture content, soil organic matter content, total nitrogen content, available phosphorus content, available potassium content, pH, electrical conductivity, ultraviolet intensity, and diurnal temperature range; the rhizosphere soil sample source information includes rhizosphere soil sample number, rhizosphere soil sample collection location, and the corresponding target native shrub name; the post-planting growth information includes survival rate, greening time, new shoot growth, and overwintering survival rate.

8. The method for propagating and improving the adaptability of native shrub seedlings suitable for high-altitude and arid regions according to claim 6, characterized in that: The process of creating qualified germplasm records and germination initiation records based on germplasm status information and target native shrub dormancy type information includes: classifying germplasm into qualified germplasm, shriveled grains, insect-damaged grains, cracks, mold spots, and plumpness based on the outline integrity, surface color difference, insect holes, cracks, mold spots, and plumpness in germplasm images or measured germplasm information, and removing shriveled grains, insect-damaged grains, damaged grains, and moldy grains; and determining the treatment items among mechanical bark breaking, warm water soaking, low temperature stratification, variable temperature stratification, and plant growth regulator soaking based on the target native shrub dormancy type information and the plumpness of qualified germplasm, and determining the corresponding treatment time, treatment temperature, stratification period, or soaking concentration for each treatment item.

9. A method for propagating and improving the adaptability of native shrub seedlings suitable for high-altitude and arid regions according to claim 6, characterized in that: At the end of each stage, a seedling stage status record is formed, specifically including: at the end of the germination and seedling establishment stage, the white sprouting rate and germination uniformity are recorded; at the end of the root system architecture culture stage, the taproot length, the number of lateral roots, and the root-to-shoot ratio are recorded; at the end of the mycotoxin-seedling symbiotic culture stage, the continued growth status of lateral roots after inoculation and the degree of leaf wilting are recorded; at the end of the stress pre-adaptation culture stage, the recovery culture time, the new shoot growth status, and the seedling stem uprightness are recorded; at the end of the substrate transition culture stage, the degree of leaf wilting, the new shoot recovery growth status, and the seedling stem uprightness are recorded under open-air conditions. The seedling stage status record is used to determine whether to enter the next adjacent stage.

10. The method for propagating and improving the adaptability of native shrub seedlings suitable for high-altitude and arid regions according to claim 6, characterized in that: During the root system architecture cultivation stage, the substrate moisture content, substrate aeration, nutrient supply, and root-promoting treatment cycle were adjusted based on the soil moisture content, soil organic matter content, total nitrogen content, available phosphorus content, and available potassium content of the target restoration plot. During the microbial-seedling co-culture stage, the functional strains used for inoculation were determined based on the low-temperature culture records, drought stress culture records, root-promoting control records, and rhizosphere recovery records of the candidate functional strains. The inoculation time and number of inoculations of the compound microbial agent were determined based on the number of lateral roots, root-shoot ratio, seedling substrate moisture content, and leaf wilting level. During the stress pre-adaptation cultivation stage, a combined stress sequence including low-temperature stress and water control stress was formed. Ultraviolet stress, diurnal temperature variation stress, or a combination of both were configured based on the ultraviolet intensity and diurnal temperature range of the target restoration plot. During the substrate transition cultivation stage, the transition substrate was configured according to the particle composition, pH, electrical conductivity, water retention capacity, and nutrient level of the soil in the target restoration plot.

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