Microorganism for decomposing organic waste and method for treating organic waste using same

Abstract

The present invention relates to a Bacillus subtilis YBK-1 strain deposited with accession number KCTC 15303 BP and a Bacillus subtilis YBK -70 strain, each decomposing organic waste, and a method for treating organic waste using the Bacillus subtilis YBK -1 strain. In addition, characterized by inoculating and culturing the Bacillus subtilis YBK-1 strain in broth for 48 hours at a temperature of 50℃ within an incubator, the method for removing organic waste using the microorganism strain of the present invention has an effect of decomposing odor and condensing water vapor generated by microorganism metabolism to discharge water of good quality.

Description

Microorganisms that decompose organic waste and methods for treating organic waste using such microorganisms

[0001] The present invention relates to Bacillus subtilis YBK-1 strain (KCTC 15303BP) and Bacillus subtilis YBK-70 strain that decompose organic waste, and a method for treating organic waste using the microorganisms. More specifically, the growth medium used for the Bacillus subtilis YBK-1 strain (KCTC 15303BP) of the present invention is Luria-Bertani (LB) liquid medium or LB-starch liquid medium.

[0002] In general, a large amount of food waste is produced from households and restaurants, and although this food waste is organic waste that can be recycled, most of it is disposed of by incineration or landfill.

[0003] Food waste has a high moisture content, so incineration requires a lot of fuel, which increases disposal costs. It can also negatively impact the atmospheric environment by producing carbon dioxide and dioxins. Landfilling also presents problems such as unpleasant odors during the decomposition process, and the amount of landfill space required for the disposal is limited. Due to these issues, research is being conducted to find ways to efficiently utilize organic waste such as food waste without incineration or landfill. In particular, active research is being conducted to utilize microorganisms in composting to make it useful in fields such as agriculture and reduce environmental pollution, achieving the double benefit of reducing the problem of environmental pollution. Based on these research results, methods for processing food waste using microorganisms have been developed, such as those in Republic of Korea Patent Nos. 10-0805036, 10-1207792, and 10-0721059.

[0004] Among them, Korean Patent No. 10-0721059 is a technology for high-temperature composting of organic waste by utilizing the salt-resistant, acid-resistant, and heat-resistant properties of Bacillus smithii KACC 91161P. It has the advantage of efficiently composting organic waste such as food waste and has an excellent reduction rate.

[0005] Organic waste is composed of easily decomposable organic matter derived from living organisms. Examples include food waste, livestock manure, and animal and plant residues. While previously considered objects to be burned or buried, scientific advancements have increasingly seen organic waste as a resource to be recycled. Especially for Korea, a resource-poor country, policies that safely recycle organic waste without negatively impacting the environment are crucial.

[0006] Food waste, a leading organic waste, has a recycling rate exceeding 90%. It is recycled into feed, fertilizer (compost), and bioenergy, but most of it is animal feed or agricultural fertilizer (compost). Food waste is primarily generated from households, restaurants, schools, military bases, and corporate meal service facilities. It consists of leftover food (30%), waste from food distribution and cooking (57%), discarded food ingredients (9%), and uneaten food (4%). Depending on the source, the amount, nature, degree of decay, and the presence of foreign substances in the waste vary in terms of hygiene management. However, because these wastes are mixed and recycled without being separated, concerns arise about the potential for livestock disease transmission through feed or fertilizer, as well as soil and water contamination, leading to reluctance to use them. Furthermore, responsibilities are divided between the Ministry of Environment and the Ministry of Agriculture, Food and Rural Affairs, creating a complex web of issues regarding organic waste disposal methods, recycling standards, and intended uses. To address this issue, the government is working to establish a systematic management system for organic waste. In relation to improving the organic waste management system, the government is pursuing the following three directions.

[0007] First, we will diversify treatment methods and scale up facilities to transition to a safe and sanitary management system acceptable to local residents. Existing small-scale wet treatment facilities, which pose a high risk of livestock infectious diseases and are vulnerable to odors and pests, should be avoided, and instead, sanitary and large-scale public treatment facilities should be expanded to ensure stable treatment. In particular, integrated biogas, which uses organic waste as a raw material and generates gas through a microbial decomposition process, integrates the treatment of food waste, livestock manure, and sewage sludge. This increases digestion efficiency and energy production, reducing operating costs and providing electricity and heat to nearby areas.

[0008] Second, the management system should be improved to ensure recycling suitability, taking into account the characteristics, emissions, and management status of each source. In particular, when recycling into feed or fertilizer, the source should be identified separately to ensure quality that meets demand. This should enable tracking of the entire process, from discharge through collection and transportation to the final recycled product. Furthermore, by establishing and thoroughly managing management and quality standards at each stage, the reliability, transparency, and safety of the resource recycling policy should be enhanced.

[0009] Finally, to fundamentally reduce the amount of organic waste discarded during food production, distribution, and consumption, we must promote environmentally friendly lifestyles, such as smart consumption habits and food waste reduction practices. Developed countries like the European Union and the United States set food waste reduction targets and encourage participation and action through various programs to curb food waste generation. Furthermore, it is necessary to expand technological development and distribution to enable hygienic, at-the-source disposal of food waste at mass catering facilities and large restaurants using food waste disposal equipment (reducers).

[0010] The Ministry of Environment is redesigning its organic waste management system to reduce public inconvenience during food waste disposal and ensure proper disposal and recycling through sanitary and safe methods. Extensive research is underway to establish a new organic waste management system that considers resource recycling and hygiene.

[0011] As a background technology of the present invention, Korean Patent Publication No. 10-1191326 relates to “Method for treating organic waste and microbial preparation therefor,” and more particularly, to a method for treating organic waste by inoculating organic waste with Bacillus smithii KCTC 3618 and Bacillus smithii ATCC 55404 and fermenting at 40 to 80°C, and to a microbial preparation for treating organic waste containing these strains. According to the present invention, organic waste such as food waste can be efficiently fermented and turned into fertilizer. The organic waste treatment method of the present invention enables continuous organic waste treatment by adding new organic waste without separate additional microbial inoculation after the organic waste is fermented after the initial inoculation of microorganisms, and in particular, it exhibits an excellent moisture reduction effect, so that subsequent use as compost or post-treatment can be performed more easily.

[0012] The present invention solves the problems of the prior art, and researches and develops a specialized strain that can eliminate food waste, livestock manure, sewage sludge, human waste, etc. without odor within 24 hours at a strain to waste ratio of 1:1, and researches and develops a system suitable for the strain's activity.

[0013]

[0014] The purpose of the present invention is to provide Bacillus subtilis YBK-1 strain (KCTC 15303BP) for decomposing and treating organic waste.

[0015] Another object of the present invention is to provide a Bacillus subtilis YBK-70 strain for decomposing organic waste.

[0016] Another object of the present invention is to provide a method for treating organic waste using a Bacillus subtilis YBK-1 strain (KCTC 15303BP) microorganism.

[0017] The organic waste-decomposing microorganisms of the present invention are characterized by being Bacillus subtilis YBK-1 (KCTC 15303BP) strain and Bacillus subtilis YBK-70 strain. The Bacillus subtilis YBK-1 strain is characterized by being deposited under the deposit number KCTC 15303BP.

[0018] In addition, the organic waste decomposing microorganism of the present invention is characterized by inoculating the microorganism Bacillus subtilis YBK-1 (Bacillus subtilis) KCTC 15303BP and culturing it in a liquid at an internal temperature of 50°C for 48 hours.

[0019] In addition, the method for treating organic waste using microorganisms of the present invention is characterized by the steps of: introducing a microbial strain and a carrier into a reaction vessel, and stirring the mixture at a temperature of 50°C with a stirring blade of the reaction vessel; a step in which organic waste is destroyed and moisture evaporates into steam by heat; a step in which steam and oil vapor are separated in the reaction vessel, and the steam rises by natural convection and collects in the internal space of the upper plate; a step in which the steam collected in the internal space flows into a condenser through a drain pipe and a pipe; a step in which the steam is converted into water in the condenser, and the steam discharged from the condenser is discharged as clear condensate.

[0020] In addition, in the organic waste treatment method using microorganisms of the present invention, the carrier is composed of sawdust, rice husk, and bamboo pieces, and the carrier is composed of 60% by volume of the sawdust, 20% by volume of the rice husk, and 20% by volume of the bamboo pieces based on the total volume; the size of the bamboo pieces is 5 to 10 cm in width and 10 to 15 cm in length; and the bamboo pieces are characterized in that when the stirring blade rotates in the reaction vessel, a plurality of the bamboo pieces help the stirring in the stirrer to secure pores in a short time, thereby enabling more water vapor to be discharged.

[0021] According to the present invention, organic waste such as livestock by-products is effectively decomposed and eliminated using the Bacillus subtilis YBK-1 microorganism and the Bacillus subtilis YBK-70 strain. Furthermore, the present invention is effective in decomposing foul odors and condensing water vapor generated by microbial metabolism to discharge high-quality water.

[0022] The organic waste destruction method using the microbial strain of the present invention has a treatment time of less than 24 hours, a destruction rate of 91% to 98%, and heavy metal emissions below the standard.

[0023] Figure 1 is a photograph showing Bacillus subtilis strain YBK-1 growing in LB medium.

[0024] Figure 2 is a photograph showing Bacillus subtilis strain YBK-70 growing in LB medium.

[0025] Figure 3 is a graph showing the activity of lipase present in the medium, i.e., released from the cells, over time after culturing Bacillus subtilis strain YBK-1 in LB liquid medium and LB-starch liquid medium.

[0026] Figure 4 is a graph measuring the activity of protease present in the medium, i.e., released from the cell, over time after culturing Bacillus subtilis strain YBK-1 in LB liquid medium and LB-starch liquid medium.

[0027]

[0028] Hereinafter, the present invention will be described in detail based on the attached drawings.

[0029] 1. Isolation and identification of microorganisms that decompose organic waste

[0030] To isolate microorganisms that decompose organic waste, 10 g of soil near Sacheon-si, Gyeongsangnam-do was cultured overnight at 80°C with 100 ml of sterile saline solution added. Then, 1 ml of the supernatant was inoculated with 100 ml of Luria-Bertani (LB) liquid exhaust, and cultured for 24 hours in a 70°C shaking incubator.

[0031] For pure isolation, 0.1 ml of the culture solution diluted 10 to 1000 times was spread on LB agar medium and cultured at 50°C for 24 hours. Among the 350 colonies, the strain with the best growth period and excellent organic waste decomposition ability was selected and named YBK-1 strain. The 16s rRNA base sequence was analyzed to identify the strain.

[0032] 2. Analysis of the 16s rRNA base sequence of the YBK-1 strain and strain identification

[0033] The 16s rRNA base sequence of the YBK-1 strain is as shown in Figure 8. The 16s rRNA base sequence of the YBK-1 strain was applied to the NCBI Blast program to compare homology (hmology).

[0034] Analysis of 16s rRNA base sequence homology showed 99.58% homology with Bacillus subtilis subsp. subtilis str.168 complete genome (NCBI Reference Sequence: NC_000964.3) (1433 / 1439). Based on these results, strain YBK-1 was named Bacillus subtilis YBK-1.

[0035] 3. Cell growth medium and culture conditions

[0036] The growth medium used for Bacillus subtilis YBK-1 strain (KCTC 15303BP) and Bacillus subtilis YBK-70 strain was Luria-Bertani (LB) liquid medium or LB starch liquid medium.

[0037] In this study, Bacillus subtilis YBK-1 strain (KCTC 15303BP), which has excellent organic waste decomposition ability, was selected as a representative strain and its characteristics were investigated. LB medium contains nutrients (carbon source, nitrogen source, inorganic salts, vitamins, etc.) necessary for microbial growth. LB-starch medium contains LB medium and 0.05% soluble starch. The seed culture was prepared in 3 mL of LB and cultured at 37°C and 200 rpm. For the main culture, the seed culture was inoculated into 50 mL of LB (1% v / v) or 50 mL of LB-starch (1% v / v) and cultured at 37°C and 200 rpm.

[0038] 4. Determining protein content

[0039] The soluble protein concentration of the growth medium was assayed using Coomassie Plus The Better Bradford Assay (TermoFisher Scientific-23238) according to the manufacturer's instructions.

[0040] 5. Amylase activity analysis

[0041] The total carbohydrate in the growth medium of each sample was quantified using the total carbohydrate assay kit (Sigma, MAK104-1KT) according to the manufacturer's instructions. The assay was prepared in a 96-well plate containing a series of glucose standards. Three technical replicates were performed for each standard and sample. The amylase activity of each sample was calculated using a standard curve.

[0042] 6. Lipase activity analysis

[0043] The assay was performed using a lipase activity assay kit (CAS number MAK046, Sigma-Aldrich). This kit uses a coupled enzyme reaction that is proportional to the enzyme activity present in the sample and can be measured colorimetrically (at 570 nm). Lipase activity was measured as the increase in 570 nm light in real time over the reaction time.

[0044] 7. Protease activity assay using fluorescein-labeled casein

[0045] Protease activity of the medium was assayed using fluorescein-labeled casein (Pierce Fluorescent Protease Assay Kit, 23266, ThermoFisher Scientific) by fluorescence resonance energy transfer (FRET) on a BioTek Synergy H1 multimode reader (Agilent).

[0046] Fluorescein excitation and emission filters were 485 and 538 nm, respectively. Fluorescein isothiocyanate-casein (FTC-casein) working reagent and trypsin standard were prepared as described in the manufacturer's instructions. The reaction mixture was incubated at 37°C for 60 minutes. Protease activity was measured by the increase in real-time fluorescence over the reaction time.

[0047] The drawing shows the results of an experiment of the present invention.

[0048] Figure 1 is a photograph showing Bacillus subtilis strain YBK-1 growing in LB medium. Bacillus subtilis strain YBK-1 grows rapidly within 24 hours.

[0049] Figure 2 is a photograph showing Bacillus subtilis strain YBK-70 growing in LB medium. Bacillus subtilis strain YBK-70 grows rapidly within 24 hours.

[0050] Figure 3 is a graph showing the activity of lipase present in the medium, i.e., released from the cells, over time after culturing Bacillus subtilis strain YBK-1 in LB liquid medium and LB starch liquid medium.

[0051] According to Figure 3, lipase is secreted the most in the early stage of culture (2 h), and as growth continues to accumulate (6 h), lipase secretion also decreases. It shows that the expression and activity of lipase are induced about twice as strongly in LB liquid medium as in LB-starch liquid medium throughout the entire growth time period. In other words, it shows that starch inhibits the expression and activity of lipase.

[0052] Figure 4 shows the activity of protease present in the medium (i.e., released from the cells) after culturing Bacillus subtilis strain YBK-1 in LB liquid medium and LB starch liquid medium over time. It shows that protease gradually accumulates as growth continues to accumulate (6 h) rather than at the beginning of the culture (2 h). It shows that the difference in protease expression between LB-starch liquid medium and LB liquid medium is not large throughout the entire growth time. In other words, starch has little effect on the expression or activity of protease.

[0053] Figure 5 is a graph measuring the activity of carbohydrate-decomposing enzyme (amylase) present in the medium (i.e., released from the cells) over time after culturing Bacillus subtilis strain YBK-1 in LB liquid medium and LB starch liquid medium. The largest amount of carbohydrate-decomposing enzyme (amylase) is released in the early stage of cultivation (2 h), and as growth continues to accumulate (6 h), the amount of carbohydrate-decomposing enzyme (amylase) released also decreases.

[0054] It shows that the expression of carbohydrate-decomposing enzyme (amylase) is induced more strongly in -starch liquid medium than in LB liquid medium. In particular, the activity of starch-decomposing enzyme increased by about 3 times during the 2h growth period. The expression pattern of lipase according to the presence or absence of starch was the exact opposite. In other words, it shows that starch strongly induces the expression or activity of carbohydrate-decomposing enzyme but inhibits the expression or activity of lipase.

[0055] Figure 6 shows a phylogenetic tree for Bacillus subtilis strain YBK-1 and Bacillus subtilis strain YBK-70 based on ribosomal small subunit rRNA (16S rRNA). The tree was generated using the Molecular Evolutionary Genetics Analysis (MEGA11) program (https: / / www.megasoftware.net / ). The phylogenetic tree shows that the two strains (YBK-1 and YBK-70) isolated from the carrier belong to Bacillus subtilis.

[0056] Figure 7 shows a phylogenetic tree for Bacillus subtilis strain YBK-1 and Bacillus subtilis strain YBK-70 based on ribosomal large subunit rRNA (23S rRNA). The phylogenetic tree was generated using the Molecular Evolutionary Genetics Analysis (MEGA11) program (https: / / www.megasoftware.net / ). The phylogenetic tree shows that the two strains (YBK-1 and YBK-70) isolated from the carrier belong to Bacillus subtilis.

[0057] <Example 1> Deposit of microbial strains

[0058] The applicant deposited the Bacillus subtilis YBK-1 strain with the Korean Collection for Type Cultures (KCTC) of the Korea Research Institute of Bioscience and Biotechnology on January 27, 2023.

[0059] The accession number is KCTC 15303BP. Bacillus subtilis, a microorganism of the genus Bacillus, is usually an aerobic microorganism. A microbial strain complex including Bacillus subtilis YBK-1 (Bacillus subtilis) microorganism has the function of decomposing organic waste, a carbon complex, with enzymes excreted by the microorganism and converting it into water (H2O). Bacillus subtilis YBK-1 KCTC 15303BP used in the present invention can be obtained from the Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology.

[0060] <Example 2> Carrier preparation and microbial culture inoculation

[0061] Inoculate the Bacillus subtilis YBK-1 (Bacillus subtilis) KCTC 15303BP microorganism deposited in the culture tank and culture at an internal temperature of 50℃ for 48 hours.

[0062] The above carrier is composed of sawdust, rice husk, and bamboo. The carrier is a mixture of 60% by volume of sawdust, 20% by volume of rice husk, and 20% by volume of bamboo, based on the total volume. 10% of a microbial sterilizing agent is adsorbed onto the carrier composed as described above. In an embodiment of the present invention, the ratio of waste to composition is 1:1.

[0063] <Example 3> Principle of organic waste decomposition and destruction

[0064] The principle of decomposition and destruction of organic waste of the present invention is as follows.

[0065] A. Microbial culture and adsorption: Microbial culture is performed by anaerobically culturing the obtained bacteria for 48 hours to create a culture solution, placing the culture solution on a carrier in an organic waste treatment plant, and stirring at 50℃ for 48 hours to ensure even adsorption onto the carrier.

[0066] B. Feed organic waste into the treatment device.

[0067] Organic waste is converted into water (H2O) by enzymes produced through the metabolic reactions of microorganisms when provided with the appropriate temperature and oxygen.

[0068] A. Organic waste is decomposed into water within 3 to 4 hours, and when the newly supplied high-pressure oxygen and low-pressure internal water vapor collide in the reactor, the oil vapor and water vapor are separated, and only the water vapor is emitted.

[0069] The discharged water vapor is discharged as clean water through the condenser, and the remaining dry air is supplied into the reaction vessel, thereby preventing bad odor.

[0070] <Example 4>

[0071] The organic waste treatment method of the present invention proceeds through the following process.

[0072] A. Step 1: Inoculate the Bacillus subtilis YBK-1 (Bacillus subtilis) KCTC 15303BP microorganism deposited in the culture tank and culture in liquid for 48 hours at an internal temperature of 50℃ in the culture tank.

[0073] B. Step 2: Add culture medium to the carrier and stir at 50℃ for 48 hours.

[0074] The carrier is composed of rice husk, rice husk, and bamboo pieces. The carrier is a mixture of 60% by volume of sawdust, 20% by volume of rice husk, and 20% by volume of bamboo, based on the total volume; 50% by volume of water is additionally mixed into the carrier and the culture medium, stirred for 48 hours at an internal temperature of 50°C, and allowed to adsorb.

[0075] The above bamboo pieces have the effect of quickly securing air gaps when the stirrer rotates inside the reaction vessel, as multiple bamboo pieces are attached to the stirrer blades and rotate together to help stir. The size of the bamboo pieces is 5 to 10 cm in width and 10 to 15 cm in length.

[0076] Step 3: Add Bacillus subtilis YBK1 (KCTC 15303BP) strain and carrier to the reaction vessel. The microbial strain can be referred to as a microbial annihilation strain.

[0077] Step 4: Stir at 50°C with a stirrer inside the reaction vessel. The air supply unit (4) supplies air into the interior of the reaction vessel (20). The steam exhaust unit (9) discharges steam inside the reaction vessel (20), and the dry waste air through the condenser is introduced back into the reaction vessel to help transport steam and prevent odor.

[0078] Step 5: This process decomposes organic waste into water within 3-4 hours in a reactor, and releases the decomposed water as steam within 24 hours, reducing waste. The remaining waste is discharged every 2-3 months.

[0079] The microbial strain according to the present invention is characterized by being capable of ensuring 24-hour destruction and composting completion with an innovative reduction of 1:1 ratio of organic waste and composition, and is a space- and time-saving, low-cost, and environmentally friendly method.

[0080] The applicant conducted the following experiment using an organic waste treatment device manufactured by the applicant installed at the Bukyung Pig Farming Cooperative's Bukyung Livestock Products Auction Center (Address: 23-100, 1403beongil, Seobu-ro, Juchon-myeon, Gimhae-si, Gyeongsangnam-do (hereinafter referred to as "Bukyung Pig Farming").

[0081] <Experimental Example 1> Treatment of 5 tons of sludge

[0082] Five tons of sludge (waste) collected from the Bukyung Pig Slaughterhouse were injected, and after 22 hours, clear condensate was discharged as a result of sludge treatment.

[0083] Organic waste generated from the organic waste treatment facility is free of impurities and has a low inorganic content, resulting in complete destruction and no residue. The treatment process involves placing waste into a reaction vessel, with a 1:1 microbial strain-to-waste ratio. Within 24 hours, the waste is broken down into water (H2O). The hydrolytic enzymes released by the microorganisms produce clear condensate. At temperatures of 50°C or 70°C, microbial metabolism generates water vapor, which then passes through a condenser to discharge the condensate, achieving a 95% to 98% reduction in the target substance.

[0084] <Experimental Example 2> Treatment of 2,500 kg of sludge cake + 2,500 kg of sludge solution

[0085] 2,500 kg of sludge cake and 2,500 kg of sludge sludge collected from the Bukyung Pig Slaughterhouse were injected into the facility. After 23 hours, clear condensate was discharged as a result of sludge and sludge treatment. Organic waste generated from the organic waste treatment facility was free of impurities and had a low inorganic content, resulting in complete destruction and no residue.

[0086] <Experimental Example 3> Processing of 5 tons of slaughter by-products

[0087] Five tons of slaughterhouse by-products collected from the Bukyung Pig Slaughterhouse were introduced, and 22 hours later, condensate was discharged as a result of the slaughterhouse by-product processing. The organic waste generated from the organic waste treatment facility was free of impurities and had a low inorganic content, resulting in complete destruction and no residue.

[0088] <Experimental Example 4> Treatment of 5 tons of talliac

[0089] Five tons of sludge collected from the Bukyung Pig Slaughterhouse were injected, and condensate was discharged after 23 hours and 30 minutes of sludge treatment. Organic waste generated from the organic waste treatment facility was free of impurities and had a low inorganic content, resulting in complete destruction and no residue.

[0090] <Experimental Example 5> Treatment of 2,500 kg of slaughter by-products + 2,500 kg of sludge

[0091] 2,500 kg of slaughter by-products and 2,500 kg of filtrate collected from the Bukyung Pig Slaughterhouse were injected into the facility. After 24 hours, clear condensate was discharged as a result of the slaughter by-products and filtrate treatment. Organic waste generated from the organic waste treatment facility was free of impurities and had a low inorganic content, resulting in complete destruction and no residue.

[0092] In Experimental Examples 1 to 5, waste was fed into the reaction vessel of an organic waste treatment device, and the ratio of microbial strain to waste was 1:1. The waste was decomposed into water (H2O) within 4 hours. The hydrolytic enzymes secreted by the microorganisms discharged condensate. The condensate is clear water that can be treated as sewage. At a temperature of 50℃ or 70℃, microbial metabolism generates water vapor, which is discharged. Only the condensate was discharged after passing through a water cooling device. More than 95% to 98% of the waste was eliminated within 24 hours.

[0093] As described above, those skilled in the art will appreciate that the present invention can be implemented in other specific forms without altering its technical spirit or essential characteristics. Therefore, the above-described embodiments are illustrative in all respects and are not intended to be limiting.

[0094] The scope of protection of the present invention is indicated by the claims described below rather than the detailed description, and design changes or modified forms derived from the meaning and scope of the claims and the equivalent concept should be interpreted as being included in the scope of protection of the present invention.

[0095] The organic waste decomposing microorganisms and the organic waste treatment method using the microorganisms of the present invention are methods that reduce the discharged food waste, livestock manure, slaughter by-products, livestock carcasses, sludge, etc. in an environmentally friendly manner and generate water to recycle nature, and thus have a very wide and large scope of future application, and are a new technology needed not only domestically but also overseas, so they have industrial applicability.

[0096] Accession number

[0097] Name of depositor: Korea Research Institute of Bioscience and Biotechnology, Biological Resource Center

[0098] Accession number: KCTC 15303BP

[0099] Date of acceptance: January 27, 2013

[0100] US NCBI registration number

[0101]

[0102] Sequence list

[0103] Shows the gene name and size registered with the National Center for Biotechnology Information (NCBI; https: / www.ncbi.nlm.nih.gov / ).

[0104] The 16S rRNA and 23S rRNA sequences of Bacillus subtilis strains YBK-1 and YBK-70 were registered with the National Center for Biotechnology Information (NCBI; https: / / www.ncbi.nlm.nih.gov / ). The GenBank accession numbers for Bacillus subtilis YBK-1 strain are OR053679 and OR053685, respectively. The GenBank accession numbers for Bacillus subtilis YBK-70 strain are OR053680 and OR053686, respectively.

Claims

1. A microorganism that decomposes organic waste, characterized in that the microorganism that decomposes organic waste is Bacillus subtilis YBK-1 (KCTC 15303BP) strain.

2. In claim 1, The above Bacillus subtilis YBK-1 strain is characterized by being deposited under the deposit number KCTC 15303BP.

3. A microorganism that decomposes organic waste, characterized in that the microorganism that decomposes organic waste is Bacillus subtilis YBK-70 strain.

4. In the organic waste disposal method, A step of inoculating Bacillus subtilis YBK-1 (KCTC 15303BP) strain in a culture medium and culturing it in liquid at an internal temperature of 50℃ for 48 hours; A step of adding culture medium to the carrier and stirring at a temperature of 50°C for 48 hours; A step of introducing the Bacillus subtilis YBK-1 (KCTC 15303BP) strain and the carrier into a reaction vessel; A step of stirring the mixture at a temperature of 50°C using a stirring blade of the above reaction vessel; The organic waste is destroyed and the moisture evaporates into water vapor by heat; A step in which water vapor and oil vapor are separated in the above reaction vessel, and water vapor rises by natural convection and collects in the internal space of the upper plate; and A method for treating organic waste using microorganisms, characterized in that the water vapor collected in the above-mentioned internal space flows to a condenser through a drain pipe and a pipe, the water vapor is converted into water in the condenser, and the water discharged from the condenser is stored in a water tank through a discharge pipe.

5. In claim 4, A method for treating organic waste using microorganisms, wherein the carrier is composed of sawdust, rice husk, and bamboo pieces, and the carrier is composed of 60% by volume of the sawdust, 20% by volume of the rice husk, and 20% by volume of the bamboo pieces based on the total volume.

6. In claim 4, The size of the above bamboo pieces is 5 to 10 cm in width and 10 to 15 cm in length; and the method for treating organic waste using microorganisms is characterized in that when the stirring blade rotates in the reaction vessel, a plurality of the bamboo pieces help to stir within the stirrer, thereby securing a gap in a short period of time, thereby enabling more water vapor to be discharged.

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