Biofilm germicide composition

Abstract

The present invention provides: a biofilm germicide composition that exhibits excellent germicidal properties against microbes present in a biofilm that has adhered to an article, especially a textile product; and a biofilm disinfection method using said composition.　This biofilm germicide composition contains the following component (a) and component (b). Component (a): (a1) a quaternary ammonium salt having a long-chain alkyl group represented by general formula (a1); and (a2) at least one compound selected from bispyridinium compounds. Component (b): (b1) a specific anionic surfactant represented by general formula (b1); and (b2) an anionic surfactant including at least one compound selected from internal olefin sulfonates having 14-20 carbon atoms.

Description

Biofilm disinfectant composition

[0001] The present invention relates to a biofilm disinfectant composition and a method for disinfecting a biofilm.

[0002] BACKGROUND ART Once bacteria attach to an object, they multiply and form a biofilm. Biofilms, also known as biofilms or slimes, generally refer to three-dimensional structures formed by microorganisms such as bacteria, enveloping them with polymeric substances such as polysaccharides, proteins, and nucleic acids produced by the microorganisms as they attach to and multiply on the surface of a material. Biofilms are a problem for individuals in a variety of situations. For example, they cause unpleasant slimy stains and foul odors in household wet environments such as kitchens, sinks, and bathroom drains. Biofilms are also prone to forming and causing problems not only in these direct water environments, but also in situations where water, moisture, and dirt coexist, such as scratches on cutting boards, the inside of dishwashing sponges, washing machine drums, and fabric products such as clothing, dish towels, dustcloths, and foot mats. Furthermore, detergents containing various surfactants are widely used to clean textile products such as clothing. In recent years, with increasing hygiene awareness among consumers, interest in bacteria and viruses adhering to the surfaces of textile products has also increased. As a result, consumers take the extra effort and expense of washing items such as socks and sweaty clothing, which are prone to bacterial growth, by soaking them in cleaning solution for a while, or by using disinfectants or bleach in combination with textile cleaners.

[0003] US Patent No. 5,728,667 discloses germicidal light-duty dishwashing detergent compositions, either gel or liquid, containing dioctyldimethylammonium salts and alkyl ether carboxylate salts.

[0004] Biofilms attached to target articles, particularly textile products, are extremely difficult to remove because the polymers that make up the biofilm function as a protective barrier that protects bacteria from various bases, and it is also extremely difficult to sterilize the bacteria present within the biofilm. The present invention provides a biofilm disinfectant composition that has excellent disinfecting properties against bacteria present within biofilms attached to target articles, particularly textile products, and a method for sterilizing biofilms using the same.

[0005] In the present invention, a biofilm disinfectant composition refers to a composition that is effective in disinfecting bacteria present in a biofilm. Also in the present invention, a method for disinfecting a biofilm refers to a method for disinfecting bacteria present in a biofilm. Also in the present invention, biofilm disinfection refers to disinfecting bacteria present in a biofilm. Also in the present invention, the perspective of biofilm disinfection refers to the perspective of further enhancing the effect of disinfecting bacteria present in a biofilm.

[0006] The present invention relates to a biofilm disinfectant composition containing the following components (a) and (b): Component (a): (a1) a compound represented by the following general formula (a1) (hereinafter referred to as component (a1)), and (a2) one or more compounds selected from bispyridinium compounds (hereinafter referred to as component (a2)):

[0007]

[0008] [In general formula (a1), R １ａ and R ２ａ are each independently a linear or branched alkyl group having 8 carbon atoms, and R ３ａ and R ４ａ are each independently a linear or branched alkyl group having 1 to 3 carbon atoms, and X － is an anion.] Component (b): An anionic surfactant R containing one or more compounds selected from (b1) a compound represented by the following general formula (b1) (hereinafter referred to as component (b1)), and (b2) an internal olefin sulfonate having from 14 to 20 carbon atoms (hereinafter referred to as component (b2)): １ｂ -O-[(PO) ｐ / (EO) ｑ ]-SO ３ M (b1) [In general formula (b1), R １ｂ is a hydrocarbon group having from 8 to 22 carbon atoms, PO is a propyleneoxy group, EO is an ethyleneoxy group, " / " is a symbol indicating that the bonding order of PO and EO does not matter, p is the average number of moles of PO added and is a number from 0 to 10, q is the average number of moles of EO added and is a number from 0 to 25, and M is a hydrogen ion, an alkali metal ion, an alkaline earth metal ion (1 / 2 atom), an ammonium ion, or an organic ammonium ion.

[0009] The present invention also relates to a method for sterilizing a biofilm, which comprises contacting an object on which a biofilm is present with the biofilm disinfectant composition of the present invention or a treatment liquid prepared by diluting the composition with water.

[0010] The present invention also relates to a method for sterilizing a biofilm, which comprises contacting a treatment liquid containing the above-mentioned components (a) and (b) with an object on which a biofilm is present.

[0011] The present invention also relates to use of a composition containing the above-mentioned components (a) and (b) for disinfecting biofilms attached to textile products or hard articles.

[0012] According to the present invention, there are provided a biofilm disinfectant composition that has excellent disinfecting properties against bacteria present in biofilms attached to target articles, particularly textile products, and a method for disinfecting biofilms using the same.

[0013] Modes for Carrying Out the Invention The reason why the biofilm disinfectant composition of the present invention and the method for disinfecting biofilms using the same have excellent disinfecting properties against bacteria present in biofilms attached to target objects, particularly textile products, is not entirely clear, but is presumed to be as follows. In the present invention, it is believed that the combined use of a specific cationic surfactant (component (a)) and a specific anionic surfactant (component (b)) allows the base to penetrate into the biofilm and act thereon, thereby enabling biofilm disinfection. Specifically, the specific cationic surfactant (component (a)) and the specific anionic surfactant (component (b)) form a loose ion complex, preventing the bactericidal component (a) from nonspecifically adsorbing to the biofilm surface, and allowing the ion complex of components (a) and (b) to penetrate into the biofilm. In addition, the penetrated ion complex has a moderate cationicity, which is thought to act on bacteria and enable disinfection. It should be noted that the present invention is not limited to the above-mentioned mechanism of action.

[0014] [Biofilm disinfectant composition] <Component (a)> The biofilm disinfectant composition of the present invention contains, as component (a), one or more compounds selected from (a1) a compound represented by the following general formula (a1) (hereinafter referred to as component (a1)), and (a2) a bispyridinium compound (hereinafter referred to as component (a2)):

[0015]

[0016] [In general formula (a1), R １ａ and R ２ａ are each independently a linear or branched alkyl group having 8 carbon atoms, and R ３ａ and R ４ａ are each independently a linear or branched alkyl group having 1 to 3 carbon atoms, and X － is an anion.]

[0017] In the compound of general formula (a1) which is the component (a1), R １ａ and R ２ａare each independently a linear or branched alkyl group having 8 carbon atoms, and from the viewpoint of biofilm sterilization, preferably a linear alkyl group having 8 carbon atoms. １ａ and R ２ａ are preferably the same as each other. ３ａ and R ４ａ are each independently a linear or branched alkyl group having 1 to 3 carbon atoms. Examples of the alkyl group having 1 to 3 carbon atoms include a group selected from a methyl group, an ethyl group, and a propyl group. － is an anion. From the viewpoint of biofilm sterilization, the anion is preferably a halogen ion or an alkyl sulfate ion having 1 to 3 carbon atoms. Examples of halogen ions include chloride ions, bromide ions, and iodide ions. X － From the viewpoint of biofilm sterilization, chloride ion is preferred. Examples of alkyl sulfate ions having 1 to 3 carbon atoms include methyl sulfate ion, ethyl sulfate ion, and propyl sulfate ion.

[0018] The component (a1) may be used alone or in combination of two or more.

[0019] The component (a2) is a bispyridinium compound. Examples of the bispyridinium compound include those described in British Patent No. 1533952, Japanese Patent Laid-Open No. 52-105228, and International Publication No. 2014 / 100807. Specifically, from the viewpoint of biofilm sterilization, the bispyridinium compound is preferably one or more selected from the group consisting of compounds represented by the following general formula (a21) and compounds represented by the following general formula (a22):

[0020]

[0021] wherein Y is an alkylene or alkenylene group having 4 to 18 carbon atoms; R ５ａeach represents an alkyl group having 6 to 18 carbon atoms, a cycloalkyl group having 5 to 7 carbon atoms, or a phenyl group with or without halogen substitution; A is an anion; q is 1 or 2, r is 1 or 2, and q×r=2.

[0022] A is a monovalent or divalent anion, such as chloride, bromide, phosphate, orthosilicate, an organic acid, such as a compound of formula R ６ａ It can be an anion from a compound such as an organic acid having —COO— or an alkyl (having 1 to 40 carbon atoms) sulfonic acid. ６ａ is hydrogen, hydroxyl, or an alkyl group having from 1 to 40 carbon atoms.

[0023] Examples of organic acids corresponding to the anion of A include acetic acid, propionic acid, phosphoric acid, glycolic acid, pyruvic acid, oxalic acid, maleic acid, malonic acid, succinic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, cinnamic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, p-toluenesulfonic acid, glycyrrhizic acid, salicylic acid, stearic acid, phosphonic acid, trifluoroacetic acid, cyanoacetic acid, 4-cyanobenzoic acid, 2-chlorobenzoic acid, 2-nitrobenzoic acid, phenoxyacetic acid, and benzenesulfonic acid.

[0024] From the viewpoint of biofilm sterilization, preferred bispyridinium compounds are compounds of general formula (a22), and further octenidine dihydrochloride (in general formula (a22), R ５ａ are n-octyl groups, Y is n-decenyl groups, A is Cl, q is 1, and r is 2; CAS number: 70775-75-6). The component (a2) may be used alone or in combination of two or more.

[0025] From the viewpoint of biofilm sterilization, the component (a) is preferably the component (a1), more preferably one or more compounds selected from dioctylmethylethylammonium salt and dioctyldimethylammonium salt, and even more preferably dioctyldimethylammonium salt.

[0026] <Component (b)> The biofilm disinfectant composition of the present invention contains, as component (b), an anionic surfactant containing one or more compounds selected from (b1) a compound represented by the following general formula (b1) (hereinafter referred to as component (b1)), and (b2) an internal olefin sulfonate salt having from 14 to 20 carbon atoms (hereinafter referred to as component (b2)). １ｂ -O-[(PO) ｐ / (EO) ｑ ]-SO ３ M (b1) [In general formula (b1), R １ｂ is a hydrocarbon group having from 8 to 22 carbon atoms, PO is a propyleneoxy group, EO is an ethyleneoxy group, " / " is a symbol indicating that the bonding order of PO and EO does not matter, p is the average number of moles of PO added and is a number from 0 to 10, q is the average number of moles of EO added and is a number from 0 to 25, and M is a hydrogen ion, an alkali metal ion, an alkaline earth metal ion (1 / 2 atom), an ammonium ion, or an organic ammonium ion.

[0027] In the compound of the general formula (b1) of the component (b1), R １ｂis a hydrocarbon group having 8 or more carbon atoms, preferably 10 or more, more preferably 12 or more, and 22 or less, preferably 20 or less, more preferably 18 or less, and even more preferably 16 or less, and is preferably a linear or branched alkyl group or a linear or branched alkenyl group, more preferably a linear or branched alkyl group, and even more preferably a linear alkyl group, from the viewpoint of biofilm sterilization. p is the average number of moles of PO added, and from the viewpoint of biofilm sterilization, it is a number that is 0 or more, preferably 0.1 or more, more preferably 0.2 or more, even more preferably 0.3 or more, still more preferably 0.5 or more, even more preferably 1 or more, still more preferably 2 or more, and 10 or less, preferably 8 or less, more preferably 5 or less, even more preferably 3 or less, and even more preferably 2.5 or less. q is the average number of moles of EO added, and from the viewpoint of biofilm sterilization, it is a number that is 0 or more, preferably 0.5 or more, more preferably 1 or more, even more preferably 2 or more, and 25 or less, preferably 8 or less, more preferably 5 or less, and even more preferably 3 or less. M is a hydrogen ion, an alkali metal ion, an alkaline earth metal ion (half an atom), an ammonium ion, or an organic ammonium ion. Examples of the alkali metal ion include a sodium ion or a potassium ion, examples of the alkaline earth metal ion (half an atom) include a calcium ion or a magnesium ion, and examples of the organic ammonium ion include alkanolamines having 1 to 3 alkanol groups each having 2 or 3 carbon atoms (e.g., monoethanolamine, diethanolamine, triethanolamine, triisopropanolamine, etc.).

[0028] Component (b2) is an internal olefin sulfonate. From the viewpoint of biofilm sterilization, the number of carbon atoms in the internal olefin sulfonate in component (b2) is preferably 14 or more, more preferably 16 or more, and preferably 24 or less, more preferably 20 or less, and even more preferably 18 or less. The number of carbon atoms in the internal olefin sulfonate in component (b2) refers to the number of carbon atoms in the internal olefin to which the sulfonate is covalently bonded.

[0029] The internal olefin sulfonate (b2) is a sulfonate obtained by sulfonating, neutralizing, and hydrolyzing a raw material internal olefin (an olefin having a double bond within the olefin chain) preferably having 10 or more carbon atoms, more preferably 14 or more carbon atoms, and preferably 20 or less, more preferably 18 or less carbon atoms. Such internal olefins also include those containing trace amounts of so-called alpha olefins (hereinafter also referred to as α-olefins), in which the double bond is located at the first position of the carbon chain. Furthermore, sulfonation of internal olefins quantitatively produces β-sultone, and a portion of the β-sultone is converted to γ-sultone and olefin sulfonic acid. These are further converted to hydroxyalkanesulfonate and olefin sulfonate in the neutralization and hydrolysis steps (e.g., J. Am. Oil Chem. Soc. 69, 39 (1992)). The hydroxy group of the resulting hydroxyalkanesulfonate is located within the alkane chain, while the double bond of the olefin sulfonate is located within the olefin chain. The resulting product is primarily a mixture of these compounds, and may contain trace amounts of hydroxyalkanesulfonates having a hydroxy group at the end of the carbon chain or olefinsulfonates having a double bond at the end of the carbon chain. In this specification, these products and their mixtures are collectively referred to as internal olefinsulfonates (component (b2)). Hydroxyalkanesulfonates are also referred to as hydroxy forms of internal olefinsulfonates (hereinafter also referred to as HAS forms), and olefinsulfonates are also referred to as olefin forms of internal olefinsulfonates (hereinafter also referred to as IOS forms). The mass ratio of the HAS form to the IOS form of the compounds in component (b2) can be measured using a high-performance liquid chromatography mass spectrometer (hereinafter abbreviated as HPLC-MS). Specifically, the mass ratio can be determined from the HPLC-MS peak area of ​​component (b2).

[0030] Examples of salts of internal olefin sulfonates include alkali metal salts, alkaline earth metal (half atom) salts, ammonium salts, and organic ammonium salts. Examples of alkali metal salts include sodium salts and potassium salts. Examples of organic ammonium salts include alkanolammonium salts containing alkanolamine and having from 2 to 6 carbon atoms.

[0031] As is clear from the above production method, the sulfonic acid group of the internal olefin sulfonate of component (b2) is present within the carbon chain of the internal olefin sulfonate, i.e., the olefin chain or alkane chain, and a small amount of the internal olefin sulfonate may contain a sulfonic acid group at the end of the carbon chain.

[0032] In component (b2), the content of internal olefin sulfonate salts in which the sulfonic acid group is present at the 5th or higher position, preferably the 5th or higher and 9th or lower position, is preferably 5% by mass or higher, more preferably 10% by mass or higher, even more preferably 15% by mass or higher, still more preferably 20% by mass or higher, and preferably 60% by mass or lower, more preferably 55% by mass or lower, even more preferably 45% by mass or lower, and still more preferably 40% by mass or lower, from the viewpoint of biofilm sterilization.

[0033] In component (b2), the mass ratio of the content of internal olefin sulfonates [hereinafter sometimes referred to as (IO-1S)] in which the sulfonic acid group is located at position 2 or more and position 4 or less to the content of internal olefin sulfonates [hereinafter sometimes referred to as (IO-2S)] in which the sulfonic acid group is located at position 5 or more, preferably position 5 or more and position 9 or less, (IO-1S) / (IO-2S), is preferably 0.5 or more, more preferably 0.8 or more, even more preferably 1.0 or more, still more preferably 1.5 or more, still more preferably 2 or more, still more preferably 2.5 or more, still more preferably 3 or more, still more preferably 4 or more, still more preferably 4.5 or more, and from the viewpoint of biofilm sterilization, is preferably 10 or less, more preferably 8 or less, even more preferably 6 or less. The contents of compounds in component (b2) in which the sulfonic acid group is located at different positions can be measured by HPLC-MS. In this specification, the content of each compound having a sulfonic acid group at a different position is determined as a mass ratio based on the HPLC-MS peak area of ​​the compound having the sulfonic acid group at each position in the total HAS form of component (b2).

[0034] The content of olefin sulfonate salts in which the sulfonic acid group is at position 1 in component (b2) is preferably 10% by mass or less, more preferably 7% by mass or less, even more preferably 5% by mass or less, and even more preferably 3% by mass or less in component (b2) from the viewpoint of biofilm sterilization, and is preferably 0.01% by mass or more from the viewpoint of reducing production costs and improving productivity. The position of the sulfonic acid group in these compounds is a position in the olefin chain or alkane chain.

[0035] The internal olefin sulfonate may be a mixture of a hydroxyl form and an olefin form. The mass ratio of the content of the olefin form in the internal olefin sulfonate to the content of the hydroxyl form in the internal olefin sulfonate (olefin form / hydroxyl form) in component (b2) may be 0 / 100 or more, further 5 / 95 or more, and may be 50 / 50 or less, further 40 / 60 or less, further 30 / 70 or less, or further 25 / 75 or less.

[0036] Examples of anionic surfactants other than component (b1) and component (b2) include one or more selected from alkylbenzenesulfonic acid, α-olefinsulfonic acid, alkanesulfonic acid, saturated or unsaturated fatty acid, alkyl or alkenyl ether carboxylic acid, α-sulfofatty acid, N-acylamino acid, mono- or diester phosphate, sulfosuccinate ester, and salts thereof. The alkyl or alkenyl group of these anionic surfactants may have, for example, 8 to 22 carbon atoms. Examples of salts of these anionic surfactants include alkali metal salts, alkaline earth metal (half atom) salts, ammonium salts, and organic ammonium salts. Examples of alkali metal salts include sodium salts and potassium salts. Examples of organic ammonium salts include alkanolammonium salts containing alkanolamine and having 2 to 6 carbon atoms.

[0037] From the viewpoint of biofilm sterilization, the component (b) is preferably an anionic surfactant containing one or more compounds selected from the group consisting of a (b1) component in which p is 0.1 or more and 10 or less and q is 0 or more and 25 or less in general formula (b1), and a (b2) component of an internal olefin sulfonate having 16 or more and 18 or less carbon atoms.

[0038] In the biofilm disinfectant composition of the present invention, the contents of the components (b1) and (b2) in the component (b) are preferably 70% by mass or more, more preferably 80% by mass or more, even more preferably 90% by mass or more, and preferably 100% by mass or less, more preferably 100% by mass, from the viewpoint of biofilm disinfection. The contents of the components (b1) and (b2) in the component (b) do not necessarily require that both the components (b1) and (b2) are essential components; it is sufficient that at least one of the components is contained. From the viewpoint of biofilm disinfection, the component (b) is preferably one or more selected from the components (b1) and (b2), and more preferably a component (b1) in which p in the general formula (b1) is 0.1 or more.

[0039] The component (b) may be used alone or in combination of two or more.

[0040] <Composition, etc.> From the viewpoint of biofilm sterilization, the biofilm disinfectant composition of the present invention contains component (a) in an amount of preferably 0.001% by mass or more, more preferably 0.01% by mass or more, even more preferably 0.1% by mass or more, still more preferably 1% by mass or more, even more preferably 3% by mass or more, even more preferably 6% by mass or more, even more preferably 9% by mass or more, even more preferably 12% by mass or more, and preferably 80% by mass or less, more preferably 70% by mass or less, even more preferably 50% by mass or less, even more preferably 30% by mass or less, even more preferably 25% by mass or less, even more preferably 20% by mass or less, and even more preferably 15% by mass or less. In the present invention, when component (a1) is included as component (a), the mass of component (a1) is used as the value converted to the chlorate salt, and when component (a2) is included as component (a), the mass of component (a2) is used as the value converted to the hydrochloride salt.

[0041] From the viewpoint of biofilm sterilization, the biofilm disinfectant composition of the present invention contains component (b) in an amount of preferably 0.001% by mass or more, more preferably 0.01% by mass or more, even more preferably 0.1% by mass or more, still more preferably 1% by mass or more, still more preferably 3% by mass or more, still more preferably 6% by mass or more, still more preferably 9% by mass or more, still more preferably 12% by mass or more, and preferably 80% by mass or less, more preferably 70% by mass or less, even more preferably 50% by mass or less, still more preferably 30% by mass or less, still more preferably 25% by mass or less, still more preferably 20% by mass or less, and still more preferably 15% by mass or less. In the present invention, the mass of component (b) is expressed as a value converted to the sodium salt.

[0042] In the biofilm disinfectant composition of the present invention, the mass ratio (a) / (b) of the content of component (a) to the content of component (b) is, from the viewpoint of biofilm disinfection, preferably 0.001 or more, more preferably 0.005 or more, even more preferably 0.01 or more, still more preferably 0.02 or more, still more preferably 0.1 or more, still more preferably 0.12 or more, still more preferably 0.15 or more, still more preferably 0.3 or more, still more preferably 0.5 or more, still more preferably 0.7 or more, and preferably 40 or less, more preferably 30 or less, still more preferably 20 or less, still more preferably 10 or less, still more preferably 7 or less, still more preferably 5 or less, still more preferably 3 or less, still more preferably 2 or less, still more preferably 1.5 or less, and still more preferably 1 or less.

[0043] The biofilm disinfectant composition of the present invention can promote biofilm disinfection by containing other components such as water-soluble solvents, chelating agents, pH adjusters, preservatives, colorants, fragrances, hydrotropes, anti-redeposition agents such as polymers, dispersants, enzymes, antibacterial agents, antioxidants, antifoaming agents, etc. (however, components (a) and (b) are excluded).

[0044] The biofilm disinfectant composition of the present invention contains water. Ion-exchanged water, tap water, purified water, etc. can be used as the water. Water can be used as the remainder of the composition in an amount such that the total composition is 100% by mass. The biofilm disinfectant composition of the present invention contains water in an amount of preferably 0.1% by mass or more, more preferably 1% by mass or more, even more preferably 5% by mass or more, and preferably 99% by mass or less, more preferably 95% by mass or less.

[0045] From the viewpoint of biofilm sterilization, the pH of the biofilm disinfectant composition of the present invention at 25°C is preferably 1 or more, more preferably 3 or more, even more preferably 5 or more, still more preferably 6 or more, and preferably 14 or less, more preferably 12 or less, and even more preferably 10 or less. The pH is a value measured at 25°C using a glass electrode. Specifically, it was measured by the following method. <pH Measurement Method> A pH electrode (BlueLine 17 pH, manufactured by SI Analytics) mounted on a Consort multi-parameter analyzer C3010 is calibrated in advance with Reagecon pH4.000 High Resolution Color Coded Buffer Solution or Reagecon pH 7.000 High Resolution Color Coded Buffer Solution, and the electrode is then thoroughly rinsed with ion-exchanged water. The pH electrode calibrated and washed as described above is placed in the biofilm disinfectant composition whose temperature has been adjusted to 25°C, and measurement is carried out using the AUTO HOLD mode of the pH meter until the measured value becomes constant.

[0046] The biofilm disinfectant composition of the present invention may be a biofilm disinfectant composition comprising component (a) and component (b), and may further contain water and other components. The matters described for the biofilm disinfectant composition of the present invention can be applied to this biofilm disinfectant composition as appropriate, and the contents and mass ratios of the components described above can be applied by replacing them with the amounts of the components.

[0047] The biofilm disinfectant composition of the present invention can be suitably used for textile products or hard articles, and more preferably for textile products.

[0048] The fibers of the textile product may be either hydrophobic or hydrophilic. Examples of hydrophobic fibers include protein-based fibers (such as milk protein casein fiber and Promix), polyamide-based fibers (such as nylon), polyester-based fibers (such as polyester), polyacrylonitrile-based fibers (such as acrylic), polyvinyl alcohol-based fibers (such as vinylon), polyvinyl chloride-based fibers (such as polyvinyl chloride), polyvinylidene chloride-based fibers (such as vinylidene), polyolefin-based fibers (such as polyethylene and polypropylene), polyurethane-based fibers (such as polyurethane), polyvinyl chloride / polyvinyl alcohol copolymer-based fibers (such as polycrelal), polyalkylene paraoxybenzoate-based fibers (such as benzoate), and polyfluoroethylene-based fibers (such as polytetrafluoroethylene). Examples of hydrophilic fibers include seed hair fibers (cotton, kapok, etc.), bast fibers (hemp, flax, ramie, hemp, jute, etc.), leaf vein fibers (Manila hemp, sisal, etc.), palm fibers, rush, straw, animal hair fibers (wool, mohair, cashmere, camel hair, alpaca, vicuna, angora, etc.), silk fibers (domestic silkworm silk, wild silkworm silk), feathers, cellulosic fibers (rayon, polynosic, cupra, acetate, etc.), etc. In the present invention, the term "textile products" refers to fabrics such as woven fabrics, knitted fabrics, and nonwoven fabrics made using the hydrophobic or hydrophilic fibers, as well as products obtained using the same, such as undershirts, T-shirts, dress shirts, blouses, slacks, hats, handkerchiefs, towels, bedding, textile products for bedding (sheets, pillowcases, etc.), knitwear, socks, underwear, tights, and masks. Preferred textile products are woven fabrics such as woven fabrics and knitted fabrics, and from the viewpoint of biofilm sterilization, preferred textile products are textile products containing hydrophobic fibers.

[0049] Examples of hard articles include washing machines, washing machine tubs, washing machine inlets, washing machine lint filters, washing machine drain pipes, tableware, hard articles around the kitchen, and / or hard articles in living environments such as bathrooms, toilet areas, and floors, drainage ditches, water pipes, food manufacturing or beverage manufacturing plants, cooling water systems such as industrial cooling towers, endoscopes, catheters, medical equipment for artificial dialysis, etc. Examples of materials for hard articles include plastics (including silicone resins, etc.) such as polyethylene and polypropylene, metals, ceramics, wood, and combinations thereof.

[0050] The biofilm disinfectant composition of the present invention has excellent disinfecting properties against bacteria present in biofilms, and can therefore be used to disinfect textile products or hard articles used not only in ordinary households but also in medical and nursing care, etc. Examples of bacteria that can be targeted by the biofilm disinfectant composition of the present invention include bacteria of the genus Moraxella, bacteria of the genus Micrococcus, bacteria of the genus Methylobacterium, bacteria of the genus Acinetobacter, bacteria of the genus Pseudomonas, bacteria of the genus Bacillus, bacteria of the genus Sphingomonas, bacteria of the genus Ralstonia, bacteria of the genus Cupriavidus, and Psychrobacter. Examples of the bacteria include one or more bacteria selected from the group consisting of bacteria of the genus Psychorobacter, bacteria of the genus Serratia, bacteria of the genus Escherichia, bacteria of the genus Staphylococcus, bacteria of the genus Burkholderia, bacteria of the genus Listeria, bacteria of the genus Shigella, bacteria of the genus Vibrio, bacteria of the genus Klebsiella, bacteria of the genus Salmonella, and bacteria of the genus Enterobacter.

[0051] [Biofilm Sterilization Method] The present invention relates to a method for sterilizing a biofilm, in which a target object on which a biofilm is present is contacted with the biofilm disinfectant composition of the present invention or a treatment solution obtained by diluting the biofilm disinfectant composition with water (hereinafter also referred to as the treatment solution of the present invention). The treatment solution of the present invention is prepared by diluting the biofilm disinfectant composition of the present invention with water, and therefore contains the components (a) and (b). That is, the present invention relates to a method for sterilizing a biofilm, in which a treatment solution containing components (a), (b), and water (hereinafter also referred to as the treatment solution of the present invention) is contacted with a target object on which a biofilm is present. Hereinafter, both the treatment solution obtained by diluting the biofilm disinfectant composition of the present invention with water and the treatment solution containing components (a), (b), and water will be referred to as the treatment solution of the present invention. In a preferred method of the biofilm sterilization method of the present invention, the biofilm disinfectant composition of the present invention or the treatment solution of the present invention is contacted with a target object on which a biofilm is present and then allowed to stand for a predetermined period of time. The term "leaving for a predetermined period of time" refers to contacting the target object on which a biofilm is present with the biofilm disinfectant composition of the present invention or the treatment solution of the present invention and then allowing the contact to continue for a predetermined period of time. From the viewpoint of enhancing the sterilization effect, the predetermined time is preferably 10 seconds or more, more preferably 30 seconds or more, even more preferably 1 minute or more, still more preferably 3 minutes or more, still more preferably 5 minutes or more, and preferably 1 hour or less, more preferably 50 minutes or less, even more preferably 40 minutes or less, still more preferably 30 minutes or less, and still more preferably 20 minutes or less. The method for sterilizing a biofilm of the present invention is carried out using the biofilm disinfectant composition of the present invention or the treatment solution of the present invention, and the aspects described for the biofilm disinfectant composition of the present invention can be applied as appropriate.

[0052] The target article of the biofilm disinfection method of the present invention is a textile product or a hard article, preferably a textile product. The aspects of the target textile product and hard article are the same as those described for the biofilm disinfectant composition of the present invention.

[0053] Methods for contacting the biofilm disinfectant composition of the present invention or the treatment liquid of the present invention with a target article on which a biofilm exists include spraying or applying the biofilm disinfectant composition of the present invention or the treatment liquid of the present invention to the target article on which a biofilm exists or is thought to exist, or immersing the target article, such as a textile product, in the biofilm disinfectant composition of the present invention or the treatment liquid of the present invention. Alternatively, the biofilm disinfectant composition of the present invention or the treatment liquid of the present invention may be impregnated into a nonwoven fabric and then contacted with the target article.

[0054] When the biofilm disinfectant composition of the present invention or the treatment liquid of the present invention is sprayed or applied to a target object, the biofilm disinfectant composition of the present invention or the treatment liquid of the present invention may be filled into a container equipped with a sprayer and sprayed in the form of droplets or foam, or the biofilm disinfectant composition of the present invention or the treatment liquid of the present invention may be poured from the container onto the target object and applied with a brush or the like. Examples of containers equipped with a sprayer include manual spray devices that do not use propellants, such as trigger-type spray containers and pump-type spray containers, and aerosols that use propellants. The container equipped with a sprayer is preferably a trigger-type spray that can spray the contents in the form of droplets or foam, and more preferably a trigger-type spray equipped with a mechanism for spraying the contents in the form of droplets or a trigger-type spray equipped with a mechanism for forming foam (foam-forming mechanism).

[0055] When a target article, such as a textile product, is immersed in the biofilm disinfectant composition of the present invention or the treatment solution of the present invention, the target article and the biofilm disinfectant composition of the present invention or the treatment solution of the present invention may be left to stand or stirred during immersion. Stirring may be performed by hand or using a rotary agitator. A rotary agitator is an apparatus that rotates the target article and the biofilm disinfectant composition of the present invention or the treatment solution of the present invention around a certain rotation axis to stir the target article and the biofilm disinfectant composition of the present invention or the treatment solution of the present invention. The rotary agitator may perform stirring in one direction only or in the opposite direction. Furthermore, stirring may be performed continuously or intermittently. Examples of rotary agitators available to users include pulsator-type washing machines, agitator-type washing machines, and drum-type washing machines. The biofilm disinfection method of the present invention preferably involves immersing a textile product on which a biofilm is present in the biofilm disinfectant composition of the present invention or the treatment solution of the present invention and stirring the textile product using a rotary agitator.

[0056] In the biofilm sterilization method of the present invention, when the target article is a hard article, after contact with the biofilm disinfectant composition of the present invention or the treatment solution of the present invention, the article may be allowed to stand or be fluidized, with fluidization being preferred from the viewpoint of further enhancing biofilm sterilization. Preferred embodiments of the method of fluidizing the biofilm disinfectant composition of the present invention or the treatment solution of the present invention over a hard article include, for example, a method of continuously or intermittently spraying the biofilm disinfectant composition or the treatment solution of the present invention over the surface of a stationary hard article, and a method of fluidizing a biofilm disinfectant composition or treatment solution of the present invention in which a hard article has been immersed. Embodiments of fluidizing a biofilm disinfectant composition or treatment solution of the present invention in which a hard article has been immersed include, for example, a method of fluidizing the biofilm disinfectant composition or treatment solution of the present invention in which a hard article has been immersed by applying an external force such as stirring, or a method of rotating or shaking a hard article immersed in the biofilm disinfectant composition or treatment solution of the present invention.

[0057] When the biofilm disinfectant composition of the present invention is diluted with water to prepare the treatment solution of the present invention, the dilution ratio is, for example, from the viewpoint of biofilm disinfection, 100,000 times or less, further 50,000 times or less, further 10,000 times or less, further 3,000 times or less, and then 10 times or more, further 50 times or more, and further 100 times or more.

[0058] From the viewpoint of biofilm sterilization, the content of component (a) in the treatment solution of the present invention is preferably 0.001 ppm or more, more preferably 0.01 ppm or more, even more preferably 0.1 ppm or more, even more preferably 1 ppm or more, and preferably 150,000 ppm or less, more preferably 100,000 ppm or less, even more preferably 50,000 ppm or less, even more preferably 10,000 ppm or less, even more preferably 5,000 ppm or less, even more preferably 1,000 ppm or less, even more preferably 500 ppm or less, even more preferably 100 ppm or less, even more preferably 50 ppm or less.

[0059] From the viewpoint of biofilm sterilization, the content of component (b) in the treatment solution of the present invention is preferably 0.001 ppm or more, more preferably 0.01 ppm or more, even more preferably 0.1 ppm or more, even more preferably 1 ppm or more, and preferably 5,000,000 ppm or less, more preferably 1,000,000 ppm or less, even more preferably 500,000 ppm or less, even more preferably 100,000 ppm or less, even more preferably 500,000 ppm or less, even more preferably 100,000 ppm or less, even more preferably 50,000 ppm or less, even more preferably 10,000 ppm or less, even more preferably 5,000 ppm or less, even more preferably 1,000 ppm or less, even more preferably 500 ppm or less, even more preferably 100 ppm or less, even more preferably 50 ppm or less.

[0060] In the treatment solution of the present invention, the ranges of mass ratios of each component described in the biofilm disinfectant composition of the present invention can be directly applied to the ranges of mass ratios of each component in the treatment solution of the present invention.

[0061] From the viewpoint of biofilm sterilization, the pH of the treatment solution of the present invention is preferably 1 or higher, more preferably 3 or higher, even more preferably 5 or higher, still more preferably 6 or higher, and preferably 14 or lower, more preferably 12 or lower, and even more preferably 10 or lower. This pH may be the pH of the treatment solution of the present invention when the temperature is 25° C. The method for measuring the pH is the same as the method described above.

[0062] From the viewpoint of biofilm sterilization, the hardness of the water used to prepare the treatment solution of the present invention is, on the German hardness scale, preferably 0° dH or more, more preferably 1° dH or more, even more preferably 3° dH or more, and preferably 30° dH or less, more preferably 25° dH or less, even more preferably 20° dH or less, still more preferably 10° dH or less, and even more preferably 5° dH or less. The hardness of the treatment solution of the present invention may be within the above-mentioned range.

[0063] Here, the German hardness (° dH) in this specification is the concentration of calcium and magnesium in water expressed as CaCO ３German hardness refers to the converted concentration of 1 mg / L (ppm) = approximately 0.056°dH (1°dH = 17.8 ppm). The calcium and magnesium concentrations for this German hardness are determined by chelate titration using disodium ethylenediaminetetraacetic acid. The specific method for measuring the German hardness of water in this specification is shown below. <Method for Measuring German Water Hardness> [Reagents] 0.01 mol / L EDTA 2Na solution: 0.01 mol / L aqueous solution of disodium ethylenediaminetetraacetate (titration solution, 0.01 M EDTA-2Na, manufactured by Sigma-Aldrich) Universal BT indicator (product name: Universal BT, manufactured by Dojindo Laboratories, Inc.) Ammonia buffer solution for hardness measurement (solution prepared by dissolving 67.5 g of ammonium chloride in 570 ml of 28 w / v% aqueous ammonia and adding ion-exchanged water to a total volume of 1000 ml) [Hardness Measurement] (1) Using a volumetric pipette, place 20 ml of sample water into a conical beaker. (2) Add 2 ml of ammonia buffer solution for hardness measurement. (3) Add 0.5 ml of Universal BT indicator. Confirm that the solution after addition is reddish purple. (4) While shaking the conical beaker well, add 0.01 mol / L EDTA 2Na solution dropwise from the burette, and the end point of the titration is when the sample water turns blue. (5) Calculate the total hardness using the following formula: Hardness (°dH) = T x 0.01 x F x 56.0774 x 100 / A T: Titration volume (mL) of 0.01 mol / L EDTA 2Na solution A: Sample volume (20 mL, volume of sample water) F: Factor of 0.01 mol / L EDTA 2Na solution

[0064] When a textile product having a biofilm is immersed in the treatment solution of the present invention, the immersion (contact) time of the textile product is preferably 10 seconds or more, more preferably 1 minute or more, even more preferably 5 minutes or more, from the viewpoint of biofilm sterilization, and preferably 1 day or less, more preferably 15 hours or less, even more preferably 5 hours or less. After immersion, the textile product is rinsed with water and dried. Rinsing may be performed using water according to known methods such as washing textile products. The water used for rinsing in the present invention is not particularly limited, but examples include tap water, well water, ion-exchanged water, sterilized water, and distilled water.

[0065] When the biofilm disinfectant composition of the present invention or the treatment liquid of the present invention is sprayed or applied to a target article, the amount of the biofilm disinfectant composition of the present invention or the treatment liquid of the present invention used is not particularly limited. For example, ２ From the viewpoint of biofilm sterilization, the amount of component (a) is preferably 0.0001 g or more, more preferably 0.001 g or more, and preferably 100 g or less, more preferably 10 g or less, per 100g of water.

[0066] When the biofilm disinfectant composition of the present invention or the treatment solution of the present invention is sprayed or applied to a target object, the time for which the biofilm disinfectant composition of the present invention or the treatment solution of the present invention is contacted with the target object on which a biofilm is present (time for leaving it) is preferably 10 seconds or more, more preferably 1 minute or more, even more preferably 5 minutes or more, and preferably 1 day or less, more preferably 15 hours or less, even more preferably 5 hours or less, from the viewpoint of biofilm disinfection. After contact, the composition may be allowed to dry as is, wiped off with a clean cloth, or rinsed with water. When rinsing, an external force (physical force) may be applied using a sponge or the like, or simply rinsed with a stream of water.

[0067] When a nonwoven fabric is impregnated with the biofilm disinfectant composition of the present invention or the treatment solution of the present invention and brought into contact with a target article, the nonwoven fabric can be processed into a sheet, and the fibers constituting the nonwoven fabric are preferably composed of one or more types of fibers selected from the hydrophilic fibers and hydrophobic fibers described above. The method for contacting the target article involves pressing a nonwoven fabric impregnated with the biofilm disinfectant composition of the present invention or the treatment solution of the present invention against the target article, applying an external force within a range that does not damage the target article, and transferring the biofilm disinfectant composition of the present invention impregnated in the nonwoven fabric, or the treatment solution obtained by diluting it with water, to the target article and bringing it into contact, and this may be done by any of rubbing, kneading, or tapping.

[0068] The biofilm disinfectant composition of the present invention is suitable for use in disinfecting biofilms attached to textile products or hard articles. That is, the present invention provides use of a composition containing the components (a) and (b) for disinfecting biofilms attached to textile products or hard articles. The composition containing the components (a) and (b) is the same as the biofilm disinfectant composition of the present invention, and specific aspects of the components (a) and (b) are also the same. Specific aspects of the composition can be appropriately applied from the aspects described for the biofilm disinfectant composition of the present invention. Furthermore, aspects described for the biofilm disinfection method of the present invention can be appropriately applied from the aspects described for the biofilm disinfectant composition of the present invention.

[0069] In addition to the above-described embodiment, the present invention discloses the following aspects.

[0070] <1> A biofilm disinfectant composition containing the following components (a) and (b): Component (a): (a1) a compound represented by the following general formula (a1) (hereinafter referred to as component (a1)), and (a2) one or more compounds selected from a bispyridinium compound (hereinafter referred to as component (a2)):

[0071]

[0072] [In general formula (a1), R １ａ and R ２ａ are each independently a linear or branched alkyl group having 8 carbon atoms, and R ３ａand R ４ａ are each independently a linear or branched alkyl group having 1 to 3 carbon atoms, and X － is an anion.] Component (b): An anionic surfactant R containing one or more compounds selected from (b1) a compound represented by the following general formula (b1) (hereinafter referred to as component (b1)), and (b2) an internal olefin sulfonate having from 14 to 20 carbon atoms (hereinafter referred to as component (b2)): １ｂ -O-[(PO) ｐ / (EO) ｑ ]-SO ３ M (b1) [In general formula (b1), R １ｂ is a hydrocarbon group having from 8 to 22 carbon atoms, PO is a propyleneoxy group, EO is an ethyleneoxy group, " / " is a symbol indicating that the bonding order of PO and EO does not matter, p is the average number of moles of PO added and is a number from 0 to 10, q is the average number of moles of EO added and is a number from 0 to 25, and M is a hydrogen ion, an alkali metal ion, an alkaline earth metal ion (1 / 2 atom), an ammonium ion, or an organic ammonium ion.

[0073] <2> In the general formula (b1) of the component (b1), R １ｂis a hydrocarbon group having 8 or more carbon atoms, preferably 10 or more, more preferably 12 or more, and 22 or less, preferably 20 or less, more preferably 18 or less, and even more preferably 16 or less, preferably a linear or branched alkyl group or a linear or branched alkenyl group, more preferably a linear or branched alkyl group, and even more preferably a linear alkyl group; p is a number of 0 or more, preferably 0.1 or more, more preferably 0.2 or more, even more preferably 0.3 or more, still more preferably 0.5 or more, even more preferably 1 or more, still more preferably 2 or more, and 10 or less, preferably 8 or less, more preferably 5 or less, even more preferably 3 or less, and even more preferably 2.5 or less; q is a number of 0 or more, preferably 0.5 or more, more preferably 1 or more, even more preferably 2 or more, and 25 or less, preferably 8 or less, more preferably 5 or less, and even more preferably 3 or less; The biofilm disinfectant composition according to <1>, wherein M is a hydrogen ion, an alkali metal ion, an alkaline earth metal ion (half an atom), an ammonium ion, or an organic ammonium ion, and is preferably a hydrogen ion, a sodium ion, a potassium ion, a calcium ion, a magnesium ion, or an alkanolamine having 1 to 3 alkanol groups having 2 or 3 carbon atoms (preferably monoethanolamine, diethanolamine, or triethanolamine).

[0074] <3> In the general formula (b1) of the component (b1), R １ｂis a linear or branched alkyl group or a linear or branched alkenyl group having from 12 to 16 carbon atoms, more preferably a linear or branched alkyl group, even more preferably a linear alkyl group; p is a number of 0.3 or more, more preferably 0.5 or more, even more preferably 1 or more, even more preferably 2 or more and 8 or less, more preferably 5 or less, even more preferably 3 or less, and even more preferably 2.5 or less; q is a number of 0 or more, preferably 0.5 or more, more preferably 1 or more, even more preferably 2 or more and 25 or less, preferably 8 or less, more preferably 5 or less, and even more preferably 3 or less; M is a hydrogen ion, an alkali metal ion, an alkaline earth metal ion (half an atom), an ammonium ion, or an organic ammonium ion, preferably a hydrogen ion, a sodium ion, a potassium ion, a calcium ion, a magnesium ion, or an alkanolamine having 1 to 3 alkanol groups having 2 or 3 carbon atoms (preferably monoethanolamine, diethanolamine, or triethanolamine).

[0075] <4> In the general formula (b1) of the component (b1), R １ｂ is a linear or branched alkyl group having from 12 to 16 carbon atoms, more preferably a linear alkyl group; p is a number of from 0.3 to 8, preferably from 0.3 to 5, more preferably from 0.5 to 3, and even more preferably from 0.5 to 2.5; q is a number of from 0 to 8, preferably from 0 to 5, more preferably from 0 to 3, and even more preferably from 0 to 2.5; and M is a hydrogen ion, an ammonium ion, a sodium ion, a potassium ion, a calcium ion, a magnesium ion, or an alkanolamine having 1 to 3 alkanol groups having 2 or 3 carbon atoms (preferably monoethanolamine, diethanolamine, or triethanolamine).

[0076] <5> The biofilm disinfectant composition according to any one of <1> to <4>, wherein the component (b2) is an internal olefin sulfonate having 16 to 18 carbon atoms.

[0077] <6> The component (b) is R in general formula (b1). １ｂ is an anionic surfactant containing one or more compounds selected from the group consisting of a component (b1) in which p is an alkyl group having from 12 to 16 carbon atoms, p is from 0.3 to 5, and q is from 0 to 3, and a component (b2) in which an internal olefin sulfonate having from 16 to 18 carbon atoms.

[0078] <7> The biofilm disinfectant composition according to any one of <1> to <6>, wherein the content of the (b1) component and the (b2) component in the (b) component is 70% by mass or more, more preferably 80% by mass or more, even more preferably 90% by mass or more, and preferably 100% by mass or less, more preferably 100% by mass.

[0079] <8> The biofilm disinfectant composition according to any one of <1> to <7>, wherein the content of the (b1) component and the (b2) component in the (b) component is 90% by mass or more and 100% by mass or less, preferably 100% by mass.

[0080] <9> The biofilm disinfectant composition according to any one of <1> to <8>, wherein the component (a2) is octenidine dihydrochloride.

[0081] <10> The biofilm disinfectant composition according to any one of <1> to <9>, wherein the mass ratio (a) / (b) of the content of the component (a) to the content of the component (b) in the biofilm disinfectant composition is 0.005 or more, more preferably 0.01 or more, even more preferably 0.02 or more, still more preferably 0.1 or more, still more preferably 0.12 or more, still more preferably 0.15 or more, still more preferably 0.3 or more, still more preferably 0.5 or more, still more preferably 0.7 or more, and 30 or less, more preferably 20 or less, still more preferably 10 or less, still more preferably 7 or less, still more preferably 5 or less, still more preferably 3 or less, still more preferably 2 or less, still more preferably 1.5 or less, still more preferably 1 or less.

[0082] <11> The biofilm disinfectant composition further contains water, and the content of component (a) in the biofilm disinfectant composition is 0.001% by mass or more, preferably 0.01% by mass or more, more preferably 0.1% by mass or more, even more preferably 1% by mass or more, still more preferably 3% by mass or more, still more preferably 6% by mass or more, still more preferably 9% by mass or more, still more preferably 12% by mass or more, and preferably 80% by mass or less, more preferably 70% by mass or less, still more preferably 50% by mass or less, still more preferably 30% by mass or less, still more preferably 25% by mass or less, still more preferably 20% by mass or less, and still more preferably 15% by mass or less. The biofilm disinfectant composition according to any one of <1> to <10>, wherein the content of the component (b) is 0.001% by mass or more, more preferably 0.01% by mass or more, even more preferably 0.1% by mass or more, still more preferably 1% by mass or more, still more preferably 3% by mass or more, still more preferably 6% by mass or more, still more preferably 9% by mass or more, still more preferably 12% by mass or more, and preferably 80% by mass or less, more preferably 70% by mass or less, even more preferably 50% by mass or less, still more preferably 30% by mass or less, still more preferably 25% by mass or less, still more preferably 20% by mass or less, and still more preferably 15% by mass or less.

[0083] <12> The biofilm disinfectant composition according to any one of <1> to <11>, which is for use in textile products.

[0084] <13> A method for sterilizing a biofilm, comprising contacting a target article on which a biofilm is present with the biofilm disinfectant composition according to any one of <1> to <12> or a treatment liquid obtained by diluting the biofilm disinfectant composition with water.

[0085] <14> The method for sterilizing a biofilm according to <13>, wherein the disinfectant composition or the treatment liquid is brought into contact with a target article on which a biofilm is present, and then the target article is left for 10 seconds to 1 hour.

[0086] <15> The method for sterilizing a biofilm according to <13> or <14>, wherein the biofilm disinfectant composition or the treatment liquid that has come into contact with the target article is caused to flow.

[0087] <16> The method for sterilizing a biofilm according to any one of <13> to <15>, wherein the target article is a textile product.

[0088] <17> A method for sterilizing a biofilm, comprising contacting a target article on which a biofilm exists with a treatment liquid containing the following component (a), component (b), and water: Component (a): (a1) one or more compounds selected from the group consisting of a compound represented by the following general formula (a1) (hereinafter referred to as component (a1)) and (a2) a bispyridinium compound (hereinafter referred to as component (a2)):

[0089]

[0090] [In general formula (a1), R １ａ and R ２ａ are each independently a linear or branched alkyl group having 8 carbon atoms, and R ３ａ and R ４ａ are each independently a linear or branched alkyl group having 1 to 3 carbon atoms, and X － is an anion.] Component (b): An anionic surfactant R containing one or more compounds selected from (b1) a compound represented by the following general formula (b1) (hereinafter referred to as component (b1)), and (b2) an internal olefin sulfonate having from 14 to 20 carbon atoms (hereinafter referred to as component (b2)):１ｂ -O-[(PO) ｐ / (EO) ｑ ]-SO ３ M (b1) [In general formula (b1), R １ｂ is a hydrocarbon group having from 8 to 22 carbon atoms, PO is a propyleneoxy group, EO is an ethyleneoxy group, " / " is a symbol indicating that the bonding order of PO and EO does not matter, p is the average number of moles of PO added and is a number from 0 to 10, q is the average number of moles of EO added and is a number from 0 to 25, and M is a hydrogen ion, an alkali metal ion, an alkaline earth metal ion (1 / 2 atom), an ammonium ion, or an organic ammonium ion.

[0091] <18> The method for sterilizing a biofilm according to <17> above, wherein the mass ratio (a) / (b) of the content of the component (a) to the content of the component (b) in the treatment solution is 0.005 or more, more preferably 0.01 or more, even more preferably 0.02 or more, still more preferably 0.1 or more, still more preferably 0.12 or more, still more preferably 0.15 or more, still more preferably 0.3 or more, still more preferably 0.5 or more, still more preferably 0.7 or more, and is 30 or less, more preferably 20 or less, even more preferably 10 or less, still more preferably 7 or less, still more preferably 5 or less, still more preferably 3 or less, still more preferably 2 or less, still more preferably 1.5 or less, and still more preferably 1 or less.

[0092] <19> The content of the component (a) in the treatment liquid is 0.001 ppm or more, preferably 0.01 ppm or more, more preferably 0.1 ppm or more, even more preferably 1 ppm or more, and 150,000 ppm or less, preferably 100,000 ppm or less, more preferably 50,000 ppm or less, even more preferably 10,000 ppm or less, still more preferably 5,000 ppm or less, still more preferably 1,000 ppm or less, still more preferably 500 ppm or less, still more preferably 100 ppm or less, still more preferably 50 ppm or less, The content of the component (b) in the treatment liquid is 0.001 ppm or more, preferably 0.01 ppm or more, more preferably 0.1 ppm or more, even 1 ppm or more, and 5,000,000 ppm or less, more preferably 1,000,000 ppm or less, even more preferably 500,000 ppm or less, still more preferably 100,000 ppm or less, still more preferably 50,000 ppm or less, still more preferably 10,000 ppm or less, still more preferably 5,000 ppm or less, still more preferably 1000 ppm or less, still more preferably 5000 ppm or less, still more preferably 100 ppm or less, still more preferably 500 ppm or less, still more preferably 100 ppm or less, still more preferably 50 ppm or less. The method for sterilizing a biofilm according to <17> or <18>.

[0093] <20> The method for sterilizing a biofilm according to any one of <17> to <19>, wherein the treatment liquid is brought into contact with the target article on which a biofilm is present, and then allowed to stand for 10 seconds or more, more preferably 30 seconds or more, even more preferably 1 minute or more, still more preferably 3 minutes or more, still more preferably 5 minutes or more, and preferably 1 hour or less, more preferably 50 minutes or less, even more preferably 40 minutes or less, still more preferably 30 minutes or less, and still more preferably 20 minutes or less.

[0094] <21> The method for sterilizing a biofilm according to any one of <17> to <20>, wherein the treatment liquid is caused to flow after contacting a target article on which a biofilm is present.

[0095] <22> Use of a composition containing the following component (a), component (b), and water for sterilizing a biofilm attached to a textile product or a hard article: Component (a): (a1) one or more compounds selected from a compound represented by the following general formula (a1) (hereinafter referred to as component (a1)), and (a2) a bispyridinium compound (hereinafter referred to as component (a2)):

[0096]

[0097] [In general formula (a1), R １ａ and R ２ａ are each independently a linear or branched alkyl group having 8 carbon atoms, and R ３ａ and R ４ａ are each independently a linear or branched alkyl group having 1 to 3 carbon atoms, and X － is an anion.] Component (b): An anionic surfactant R containing one or more compounds selected from (b1) a compound represented by the following general formula (b1) (hereinafter referred to as component (b1)), and (b2) an internal olefin sulfonate having from 14 to 20 carbon atoms (hereinafter referred to as component (b2)): １ｂ -O-[(PO) ｐ / (EO) ｑ ]-SO ３ M (b1) [In general formula (b1), R １ｂ is a hydrocarbon group having from 8 to 22 carbon atoms, PO is a propyleneoxy group, EO is an ethyleneoxy group, " / " is a symbol indicating that the bonding order of PO and EO does not matter, p is the average number of moles of PO added and is a number from 0 to 10, q is the average number of moles of EO added and is a number from 0 to 25, and M is a hydrogen ion, an alkali metal ion, an alkaline earth metal ion (1 / 2 atom), an ammonium ion, or an organic ammonium ion.

[0098] <23> In the general formula (b1) of the component (b1), R １ｂis a linear or branched alkyl group having from 12 to 16 carbon atoms, more preferably a linear alkyl group, p is a number of from 0.3 to 8, preferably from 0.3 to 5, more preferably from 0.5 to 3, and even more preferably from 0.5 to 2.5, q is a number of from 0 to 8, preferably from 0 to 5, more preferably from 0 to 3, and still more preferably from 0 to 2.5, and M is a hydrogen ion, an ammonium ion, a sodium ion, a potassium ion, a calcium ion, a magnesium ion, or an alkanolamine having 1 to 3 alkanol groups having 2 or 3 carbon atoms (preferably monoethanolamine, diethanolamine, or triethanolamine), for sterilizing a biofilm attached to a textile product or a hard article.

[0099] <24> Use of the composition according to <22> or <23>, wherein the component (b2) is an internal olefin sulfonate having 16 to 18 carbon atoms, for disinfecting a biofilm attached to a textile product or a hard article.

[0100] <25> The component (b) is R in general formula (b1). １ｂ is an anionic surfactant containing one or more compounds selected from the group consisting of a (b1) component (b1) which is an alkyl group having from 12 to 16 carbon atoms, p is from 0.3 to 5, and q is from 0 to 3, and a (b2) component which is an internal olefin sulfonate having from 16 to 18 carbon atoms, and a use of the composition according to any one of <22> to <24> above for sterilizing a biofilm attached to a textile product or a hard article.

[0101] <26> Use of the composition according to any one of <22> to <25>, wherein the content of the component (b1) and the component (b2) in the component (b) is 70% by mass or more, more preferably 80% by mass or more, even more preferably 90% by mass or more, and preferably 100% by mass or less, more preferably 100% by mass or less.

[0102] <27> Use of the composition according to any one of <22> to <26>, wherein the content of the component (b1) and the component (b2) in the component (b) is 90% by mass or more and 100% by mass or less, preferably 100% by mass, for sterilizing a biofilm attached to a textile product or a hard article.

[0103] <28> Use of the composition according to any one of <22> to <27>, wherein the component (a2) is octenidine dihydrochloride, for disinfecting a biofilm attached to a textile product or a hard article.

[0104] <29> Use of the composition according to any one of <22> to <28> above for sterilizing a biofilm attached to a textile product or a hard article, wherein the mass ratio (a) / (b) of the content of the component (a) to the content of the component (b) in the composition is 0.005 or more, more preferably 0.01 or more, even more preferably 0.02 or more, still more preferably 0.1 or more, still more preferably 0.12 or more, still more preferably 0.15 or more, still more preferably 0.3 or more, still more preferably 0.5 or more, still more preferably 0.7 or more, and is 30 or less, more preferably 20 or less, even more preferably 10 or less, still more preferably 7 or less, still more preferably 5 or less, still more preferably 3 or less, still more preferably 2 or less, still more preferably 1.5 or less, and still more preferably 1 or less.

[0105] Examples [Components] The following components were used in the examples and comparative examples. <Component (a)> Q-D08: dioctyldimethylammonium chloride, in which R １ａ and R ２ａ is an alkyl group having 8 carbon atoms, and R ３ａ and R ４ａ is a methyl group, and X － is a chloride ion, component (a1) octenidine: octenidine dihydrochloride, in general formula (a22), R ５ａare n-octyl groups, Y is n-decenyl groups, A is Cl, q is 1, and r is 2. Component (a2), trade name "Octenidine Dihydrochloride", manufactured by Tokyo Chemical Industry Co., Ltd. <Component (a') (comparison component for component (a)> C10DMAB: didecyldimethylammonium bromide, in the general formula (a1), R １ａ and R ２ａ is an alkyl group having 10 carbon atoms, and R ３ａ and R ４ａ is a methyl group, and X － is a bromo ion, trade name "Didecyldimethylammonium Bromide", manufactured by Tokyo Chemical Industry Co., Ltd. Q-D10: didecyldimethylammonium chloride, in which R １ａ and R ２ａ is an alkyl group having 10 carbon atoms, and R ３ａ and R ４ａ is a methyl group, and X － is a chloride ion

[0106] <Component (b)> AS: alkyl sulfate ester salt, in general formula (b1), R １ｂ is an alkyl group having 12 carbon atoms, p is 0, q is 0, and M is a sodium ion, component (b1), trade name "sodium dodecyl sulfate", manufactured by Tokyo Chemical Industry Co., Ltd. ApS: partial PO-added alkyl ether sulfate, in general formula (b1), R １ｂ is an alkyl group having 8 to 12 carbon atoms, p is 0.6, q is 0, and M is a sodium ion, component (b1) ES: polyoxyethylene alkyl ether sulfate, in general formula (b1), R １ｂ is an alkyl group having 10 to 16 carbon atoms, p is 0, q is 2, and M is a sodium ion, component (b1), trade name "EMAL 227", manufactured by Kao Corporation. C12APES: a polyoxypropylene polyoxyethylene alkyl ether sulfate having an alkyl group with 12 carbon atoms, in general formula (b1), R １ｂis an alkyl group having 12 carbon atoms, p is 2, q is 2, and M is monoethanolammonium, component (b1) C16IOS: potassium salt of an internal olefin sulfonate having 16 carbon atoms, component (b2) <component (b') (comparison component of component (b)> LAS (linear alkylbenzene sulfonate), trade name "Neopelex G-25", manufactured by Kao Corporation RLM45NV: sodium polyoxyethylene (4.5) lauryl ether acetate (the number in parentheses is the average number of moles of oxyethylene groups added) Sodium stearate

[0107] [Preparation of biofilm disinfectant composition] The preparation method of the biofilm disinfectant composition shown in Tables 1 to 7 is as follows. A 3 cm long Teflon stirrer piece was placed in a 200 mL glass beaker, followed by 10 g of ion-exchanged water, and the components (a) or (a'), (b) or (b') were added in the formulations shown in Tables 1 to 7. The mixture was stirred at 100 r / min for 10 minutes, and 95% by mass of the amount of ion-exchanged water needed to be added to make the total amount 100 g was added. After stirring at 100 r / min for 5 minutes, the pH (25 ° C.) was adjusted to 8 with monoethanolamine or hydrochloric acid (Examples 35 and 36 in Table 7 were adjusted to the pH listed in the table). The total amount was 100 g. The ion-exchanged water was added, and the mixture was stirred at 100 r / min for 15 minutes. The biofilm disinfectant composition shown in Tables 1 to 7 was prepared. After preparation, the pH was checked again, and the pH of each biofilm disinfectant composition in Tables 1 to 7 was found to be unchanged from the pH at the time of preparation. The amounts of each component in each biofilm disinfectant composition shown in Tables 1 to 7 are all active components. For Example 29 in Table 4, the amounts of each component in the diluted test treatment solution are shown below.

[0108] [Biofilm disinfection evaluation] (1-1) Preparation of biofilm-formed fabrics Glycerol stock solutions of Rhodotorula mucilaginosa (clothing isolate) and Methylobacterium variable (clothing isolate) were smeared on potato dextrose agar medium (manufactured by Difco Laboratories) and statically cultured for 2 days at 30 ° C. A glycerol stock solution of Moraxella osloensis (clothing isolate) was smeared on SCD agar medium (Shiotani MS Co., Ltd. SCD agar medium "Daigo", for general bacterial testing) and statically cultured for 1 day at 37 ° C. After culturing, the bacterial cells were scraped off with a disposable loop (As One Corporation, Disposable Loop 10 Type) and suspended in R2A medium (Shiotani MS Corporation, R2A medium "Daigo"). The turbidity (OD600) of each bacterial suspension was measured using a spectrophotometer (Apel Corporation, Model No. PD-303S). The bacterial solution was diluted to an OD600 of 0.2 for R. mucilaginosa and 0.01 for M. variable and M. osloensis. Hiraori cotton cloth (Yagashira Shoten, Cotton 2003) cut into 3 x 3 cm pieces was autoclaved in an autoclave (PHC Corporation, High-Pressure Steam Sterilizer, Lab Autoclave, Model No. MLS-3781). The sterilized Hiraori cotton cloth was then placed in the No. The samples were placed in a No. 6 screw tube (Maruem Corporation, screw tube bottle No. 6), and 1 mL of each of the three bacterial strains and 7 mL of R2A medium were added. The tubes were then sealed with a lid and cultured for two days at 30°C and 200 rpm in a small constant-temperature shaking incubator, Bioshaker (TAITEC Corporation, model number: BR-23FP). After culturing, the Hiraori cotton fabric was transferred to a new No. 6 screw tube, and 10 mL of autoclave-sterilized ion-exchanged water was added. The fabric was washed by shaking for 3 minutes at 25°C and 150 rpm in a small constant-temperature shaking incubator, Bioshaker. After washing, the Hiraori cotton fabric was air-dried for 1 hour in a safety cabinet to prepare a biofilm-forming fabric. Biofilm formation on the fabric was confirmed by loosening the fabric into threads with tweezers, staining the threads, and observing them with a confocal laser scanning microscope (LSM880 ZEN, manufactured by Carl Zeiss KK).The threads to be observed were stained with a dye for staining live cells (C375-Cellstain™-CFSE, manufactured by Dojindo Laboratories, Inc.), a dye for staining dead cells (P378-Cellstain™-PI solution, manufactured by Dojindo Laboratories, Inc.), a dye for staining cellulose (Calcofluor White staining solution, manufactured by Sigma-Aldrich), a dye for staining sugars (Lectin PNA From Arachis hypogaea (peanut), Alexa Fluor™ 647 Conjugate, manufactured by Thermo Fisher Scientific Co., Ltd.), a dye for staining sugars (Concanavalin Alexa Fluor™ 647, manufactured by Thermo Fisher Scientific Co., Ltd.), and a dye for staining sugars (Lectin PNA From Arachis hypogaea (peanut), Alexa Fluor™ 647 Conjugate, manufactured by Thermo Fisher Scientific Co., Ltd.). The cells were stained with HCl (Conjugate) for 30 minutes.

[0109] (1-2) Preparation of biofilm-forming hard surfaces For Example 31 in Table 5, 0.3 mL of each of the three bacterial species and 2.1 mL of R2A medium were added per well of a polystyrene 6-well plate (Corning Falcon Cell Culture 6-well multiwell plate with flat bottom lid), and the plate was sealed with a lid and allowed to stand at 30 °C for 2 days. After incubation, the upper layer of the 6-well plate was absorbed and air-dried for 1 hour in a safety cabinet to form a biofilm-forming hard surface. Biofilm formation on the hard surface was confirmed by observation with a digital microscope (Keyence Corporation VHX-7000).

[0110] (2) Biofilm Sterilization Test (Biofilm Sterilization Evaluation) The prepared biofilm-formed cloth was cut into 1.5 × 1.5 cm pieces, and the sterilized Hiraori cotton cloth was cut into 1.5 × 3 cm pieces. A total of three pieces, one cut biofilm-formed cloth and two cut sterilized Hiraori cotton cloths, were stacked and placed in a 5 mL tube (Eppendorf Tube 5.0 mL, model number: 0030 119.401, manufactured by Eppendorf Co., Ltd.). To this was added 3 mL of a test treatment solution prepared by diluting each of the biofilm disinfectant compositions in Tables 1 to 7 3000 times with model tap water (4 ° dH) (in Table 4, Example 29 used the test treatment solution diluted to the concentration listed in the table, and Example 30 was used as is at the concentration listed in the table without dilution. For Examples 32, 33, and 34 in Table 6, the hardness of the model tap water for dilution 3000 times was changed to that listed in the table to prepare a test treatment solution.) The solution was then washed by shaking in a small thermostatic shaking incubator bioshaker at 25 ° C. and 150 rpm for 10 minutes, which was the washing step. The washed cloth was transferred to a 5 mL tube containing 3 mL of model tap water and washed by shaking for 3 minutes under the same conditions, which was the rinsing step. This washing and rinsing step was repeated four times, after which only the biofilm-formed cloth was removed and air-dried in a safety cabinet for 30 minutes. For biofilm-forming hard surfaces, 3 mL of the test treatment solution prepared by diluting the biofilm disinfecting composition of Example 31 in Table 5 30,000 times with model tap water (4 ° dH) was added per well, and the well was shaken and washed for 10 minutes at 25 ° C and 150 rpm in a small constant temperature shaking incubator bioshaker. This was the washing step. The test treatment solution in the bioshaker after washing was aspirated with a micropipette (Eppendorf Research (R) plus, manufactured by Eppendorf Co., Ltd.), and 3 mL of model tap water was added. The well was shaken and washed for 3 minutes under the same conditions. This rinsing step was repeated four times, and the model tap water in the bioshaker after rinsing was then aspirated with a micropipette. The rinsed biofilm-forming hard surface was then air-dried for 30 minutes in a safety cabinet.

[0111] (Measurement of the number of remaining viable bacteria) The biofilm-formed fabric after air drying and the biofilm-formed fabric before contact with the test treatment solution were each placed in a 1.5 mL tube (Eppendorf Co., Ltd., Eppendorf tube 1.5 mL, model number: 0030 125.150) containing 1 mL of LP dilution solution (Shiotani MS Co., Ltd., LP dilution solution "Daigo"). With the lid on, the tube was placed in an ultrasonic cleaner (Emerson Japan Co., Ltd., tabletop ultrasonic cleaner, model number: 2510JDTH) for 15 minutes, and the bacteria remaining on the fabric were dispersed in the LP dilution solution. After stirring the solution for 3 seconds using a vortex mixer (Vortex Mixer, Model No. VTX-3000L, manufactured by AS ONE Corporation), the bacterial solution was serially diluted 10-fold with the LP dilution solution and 100 μL of each solution was dispensed into a petri dish (Aznol Petri dish, 90 mm diameter, 15 mm height, manufactured by AS ONE Corporation). Approximately 15 mL of SCD agar medium was added, the dish was covered, gently mixed, and then left to stand at room temperature until the agar solidified. After static culture at 37 °C for 1 day, the number of remaining viable M. variable bacteria was counted using a colony counter pen (Colony Counter Pen Type, manufactured by AS ONE Corporation, Model No. 2-5764-01). For the biofilm-forming hard surfaces, 3 mL of the LP dilution solution was added per well to the air-dried biofilm-forming hard surface and to the biofilm-forming hard surface before contact with the test treatment solution. The biofilm was detached from the wells by pipetting 20 times with a micropipette and dispersed in the LP dilution solution. This bacterial solution was serially diluted 10-fold with the LP dilution solution, and the number of remaining viable bacteria of M. variable was counted in the same manner as above. (Method for calculating biofilm bactericidal activity value) The biofilm bactericidal activity value was calculated using the following formula. The results are shown in 1 to 7. The higher the bactericidal activity value, the better the biofilm bactericidal ability. Biofilm bactericidal activity value = Log A - Log B A: average number of remaining viable bacteria before contact with the test treatment solution B: average number of remaining viable bacteria after contact with the test treatment solution

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Claims

1. A biofilm disinfectant composition comprising the following components (a) and (b): Component (a): (a1) a compound represented by the following general formula (a1) (hereinafter referred to as component (a1)), and (a2) one or more compounds selected from a bispyridinium compound (hereinafter referred to as component (a2)): [In general formula (a1), R １ａ and R ２ａ are each independently a linear or branched alkyl group having 8 carbon atoms; R ３ａ and R ４ａ are each independently a linear or branched alkyl group having 1 to 3 carbon atoms; － is an anion.] Component (b): (b1) an anionic surfactant containing one or more compounds selected from a compound represented by the following general formula (b1) (hereinafter referred to as component (b1)) and (b2) an internal olefin sulfonate having from 14 to 20 carbon atoms (hereinafter referred to as component (b2)). １ｂ -O-[(PO) ｐ / (EO) ｑ ]-SO ３ M (b1) [In general formula (b1), R １ｂ is a hydrocarbon group having 8 to 22 carbon atoms, PO is a propyleneoxy group, EO is an ethyleneoxy group, " / " is a symbol indicating that the bonding order of PO and EO does not matter, p is the average number of moles of PO added and is a number of 0 to 10, q is the average number of moles of EO added and is a number of 0 to 25, and M is a hydrogen ion, an alkali metal ion, an alkaline earth metal ion (1 / 2 atom), an ammonium ion, or an organic ammonium ion.

2. A biofilm disinfectant composition as described in claim 1, wherein the (b) component is an anionic surfactant containing one or more compounds selected from the (b1) component in which p in the general formula (b1) is 0.1 or more and 10 or less and q is 0 or more and 25 or less, and the (b2) component of an internal olefin sulfonate having a carbon number of 16 or more and 18 or less.

3. A biofilm disinfectant composition described in claim 1 or 2, wherein the content of the (b1) component and the (b2) component in the (b) component is 70 mass% or more and 100 mass% or less.

4. A biofilm disinfectant composition described in any one of claims 1 to 3, wherein component (a2) is octenidine dihydrochloride.

5. A biofilm disinfectant composition described in any one of claims 1 to 4, wherein the mass ratio (a) / (b) of the content of the component (a) to the content of the component (b) is 0.005 or more and 30 or less.

6. A biofilm disinfectant composition described in any one of claims 1 to 5, further containing water, the content of component (a) being 0.001 mass% or more and 15 mass% or less, and the content of component (b) being 0.001 mass% or more and 80 mass% or less.

7. A biofilm disinfectant composition according to any one of claims 1 to 6, which is for use on textile products.

8. A method for disinfecting a biofilm, comprising contacting a target article on which a biofilm is present with a biofilm disinfectant composition according to any one of claims 1 to 7, or a treatment liquid obtained by diluting the composition with water.

9. A method for disinfecting a biofilm as described in claim 8, wherein the biofilm disinfectant composition or the treatment liquid is brought into contact with a target object on which a biofilm is present and then left for 10 seconds to 1 hour.

10. A method for sterilizing a biofilm as described in claim 8 or 9, wherein the biofilm disinfectant composition or the treatment liquid that has come into contact with the target item is caused to flow.

11. The method for sterilizing a biofilm described in any one of claims 8 to 10, wherein the target article is a textile product.

12. A method for sterilizing a biofilm, comprising contacting an object on which a biofilm exists with a treatment liquid containing the following components (a) and (b), and water: Component (a): (a1) one or more compounds selected from a compound represented by the following general formula (a1) (hereinafter referred to as component (a1)), and (a2) a bispyridinium compound (hereinafter referred to as component (a2)): [In general formula (a1), R １ａ and R ２ａ are each independently a linear or branched alkyl group having 8 carbon atoms; R ３ａ and R ４ａ are each independently a linear or branched alkyl group having 1 to 3 carbon atoms; － is an anion.] Component (b): (b1) an anionic surfactant containing one or more compounds selected from a compound represented by the following general formula (b1) (hereinafter referred to as component (b1)) and (b2) an internal olefin sulfonate having from 14 to 20 carbon atoms (hereinafter referred to as component (b2)). １ｂ -O-[(PO) ｐ / (EO) ｑ ]-SO ３ M (b1) [In general formula (b1), R １ｂ is a hydrocarbon group having 8 to 22 carbon atoms, PO is a propyleneoxy group, EO is an ethyleneoxy group, " / " is a symbol indicating that the bonding order of PO and EO does not matter, p is the average number of moles of PO added and is a number of 0 to 10, q is the average number of moles of EO added and is a number of 0 to 25, and M is a hydrogen ion, an alkali metal ion, an alkaline earth metal ion (1 / 2 atom), an ammonium ion, or an organic ammonium ion.

13. A method for sterilizing a biofilm as described in claim 12, wherein the mass ratio (a) / (b) of the content of component (a) to the content of component (b) in the treatment liquid is 0.005 or more and 30 or less.

14. A method for sterilizing a biofilm as described in claim 12 or 13, wherein the treatment liquid is brought into contact with a target object on which a biofilm exists and then left for 10 seconds to 1 hour.

15. Use of a composition containing the following components (a) and (b) for disinfecting biofilms attached to textile products or hard articles: Component (a): (a1) one or more compounds selected from a compound represented by the following general formula (a1) (hereinafter referred to as component (a1)) and (a2) a bispyridinium compound (hereinafter referred to as component (a2)) [In general formula (a1), R １ａ and R ２ａ are each independently a linear or branched alkyl group having 8 carbon atoms; R ３ａ and R ４ａ are each independently a linear or branched alkyl group having 1 to 3 carbon atoms; － is an anion.] Component (b): (b1) an anionic surfactant containing one or more compounds selected from a compound represented by the following general formula (b1) (hereinafter referred to as component (b1)) and (b2) an internal olefin sulfonate having from 14 to 20 carbon atoms (hereinafter referred to as component (b2)). １ｂ -O-[(PO) ｐ / (EO) ｑ ]-SO ３ M (b1) [In general formula (b1), R １ｂ is a hydrocarbon group having 8 to 22 carbon atoms, PO is a propyleneoxy group, EO is an ethyleneoxy group, " / " is a symbol indicating that the bonding order of PO and EO does not matter, p is the average number of moles of PO added and is a number of 0 to 10, q is the average number of moles of EO added and is a number of 0 to 25, and M is a hydrogen ion, an alkali metal ion, an alkaline earth metal ion (1 / 2 atom), an ammonium ion, or an organic ammonium ion.

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