Agent for controlling disease in small grain cereals and method for controlling disease in small grain cereals

Abstract

This seed treatment agent or soil treatment agent for controlling blight in small grain cereals is highly safe for small grain cereals, simple and efficient with a small labor load, and capable of exhibiting an excellent blight control effect. Said agent contains probenazole as an active ingredient. This method for controlling blight in small grain cereals includes treating, before sowing seeds of small grain cereals, the seeds or soil using the seed treatment agent or soil treatment agent for controlling blight in small grain cereals.

Description

Wheat disease control agent and wheat disease control method

[0001] The present invention relates to a seed treatment agent for controlling rice blast in wheat and barley, a soil treatment agent for controlling rice blast in wheat and barley, and a method for controlling rice blast in wheat and barley.This application claims priority based on Japanese Patent Application No. 2023-118713, filed on July 20, 2023, the contents of which are incorporated herein by reference.

[0002] Pyricularia oryzae, a filamentous fungus that causes rice blast disease in grasses, has differentiated into host-specific fungal groups, including rice fungi, foxtail millet fungi, finger millet fungi, and oat fungi. Among these, the rice fungus is the most well-known, and rice blast disease caused by it is known as the most serious rice disease. Meanwhile, wheat blast disease has recently become a global problem. In 1985, wheat blast disease suddenly occurred in Brazil. The pathogen was P. oryzae, the same species as the rice blast fungus, but its parasitic properties were completely different, and it was pathogenic only to Triticum spp. It was discovered that a new fungal group (wheat blast fungus) that specifically attacks Triticum spp. had evolved and emerged (Non-Patent Document 1). This fungus primarily infects panicles, causing whitening of the ears. In many cases, the disease exhibits a unique outbreak pattern, affecting entire fields simultaneously, resulting in significant yield losses. Wheat blast subsequently spread to neighboring countries such as Bolivia, Paraguay, and Argentina, becoming one of the most serious wheat diseases in South America. The disease remained confined to the Americas for 30 years after its first outbreak in Brazil, but in 2016, wheat blast suddenly emerged in Bangladesh. The disease rapidly spread throughout Bangladesh, causing a 90% yield loss in over 15,000 hectares of wheat fields and dealing a major blow to wheat cultivation in the country. This was the first wheat blast outbreak in Asia and Eurasia. Furthermore, in 2018, wheat blast occurred in Zambia, Africa. Thus, the disease is now exhibiting pandemic potential (Non-Patent Document 2).

[0003] IGARASHI, S., and 4 others, “Pyricularia sp. em trigo. I. Ocorrencia de Pyricularia sp. no estado do Parana.”, Fitopatologia Brasileira, Brasilia, 1986, Vol. 11, No. 2, p. 351-352 Tosa, Y., “Toward development of resistant lines against a transboundary plant disease: wheat blast.” Journal of General Plant Pathology, 2021, Vol. 87, No. 6, p. 394-397

[0004] An object of the present invention is to provide a seed treatment agent for controlling blast disease in wheat and barley, a soil treatment agent for controlling blast disease in wheat and barley, and a method for controlling blast disease in wheat and barley, which are highly safe to wheat and barley, simple and efficient, have a low labor load, and are capable of exerting an excellent blast control effect.

[0005] [1] A seed treatment agent for controlling blast disease in wheat or barley, comprising probenazole as an active ingredient. [2] The seed treatment agent for controlling blast disease in wheat or barley according to [1], wherein the wheat or barley is wheat or barley. [3] The seed treatment agent for controlling blast disease in wheat or barley according to [1], wherein the blast disease in wheat or barley is wheat blast or barley blast. [4] Use of a composition containing probenazole as an active ingredient as a seed treatment agent for controlling blast disease in wheat or barley. [5] The use according to [4], wherein the wheat or barley is wheat or barley. [6] The use according to [4], wherein the blast disease in wheat or barley is wheat blast or barley blast. [7] A wheat or barley seed having probenazole adhered to at least a portion of its surface. [8] The wheat or barley seed according to [7], wherein the wheat or barley is wheat or barley. [9] A method for controlling blast in wheat or barley, comprising treating seeds with the seed treatment agent for controlling blast in wheat or barley according to any one of [1] to [3] before sowing.

[10] A soil treatment agent for controlling blast in wheat or barley, comprising probenazole as an active ingredient.

[11] The soil treatment agent for controlling blast in wheat or barley according to

[10] , wherein the wheat or barley is wheat or barley.

[12] The soil treatment agent for controlling blast in wheat or barley according to

[10] , wherein the blast in wheat or barley is wheat blast or barley blast.

[13] Use of a composition containing probenazole as an active ingredient as a soil treatment agent for controlling blast in wheat or barley.

[14] The use according to

[13] , wherein the wheat or barley is wheat or barley.

[15] The use according to

[13] , wherein the blast disease of wheat or barley is wheat blast or barley blast.

[16] A method for controlling blast disease of wheat or barley, comprising treating soil with the soil treatment agent for controlling blast disease of wheat or barley according to any one of

[10] to

[12] before sowing seeds of wheat or barley.

[0006] According to the present invention, it is possible to provide a seed treatment agent for controlling blast disease in wheat and barley, a soil treatment agent for controlling blast disease in wheat and barley, and a method for controlling blast disease in wheat and barley, which are highly safe to wheat and barley, simple and efficient with a low labor load, and which can exert an excellent blast control effect.

[0007] Furthermore, the seed treatment agent for controlling blast disease in wheat and barley, the soil treatment agent for controlling blast disease in wheat and barley, and the method for controlling blast disease in wheat and barley of the present invention can achieve sufficient effects with low concentrations of the active ingredient, and are therefore highly safe in that the risks of environmental exposure and worker exposure are low.

[0008] The following describes in detail the embodiments of the present invention. However, the present invention is not limited to the embodiments described below, and various modifications are possible without departing from the gist of the present invention.

[0009] In the present invention, in the neighbor-joining unrooted phylogenetic tree of Figure 3 in Gladieux et al. (2018), those included in the Triticum lineage are treated as wheat fungi, those included in the Oryza lineage are treated as rice fungi, and those included in the Lolium lineage are treated as oat fungi. (Reference) Gladieux, Pierre et al., Gene Flow between Divergent Cereal- and Grass-Specific Lineages of the Rice Blast Fungus Magnaporthe oryzae, mBio, 2018, Vol. 9, No. 1, e01219-17.

[0010] [Seed treatment agent for controlling blast disease in wheat and barley and soil treatment agent for controlling blast disease in wheat and barley] The seed treatment agent for controlling blast disease in wheat and barley (hereinafter also simply referred to as "seed treatment agent") or soil treatment agent for controlling blast disease in wheat and barley (hereinafter also simply referred to as "soil treatment agent") of the first embodiment of the present invention contains probenazole as an active ingredient. The seed treatment agent for controlling blast disease in wheat and barley or the soil treatment agent for controlling blast disease in wheat and barley of this embodiment may consist only of probenazole. Alternatively, the seed treatment agent for controlling blast disease in wheat and barley or the soil treatment agent for controlling blast disease in wheat and barley of this embodiment may be a composition containing an ingredient other than probenazole.

[0011] Probenazole (CAS registration number 27605-76-1; IUPAC name 3-allyloxy-1,2-benzisothiazole 1,1-dioxide) is a compound represented by formula (I) and is an agricultural fungicide used as a preventative agent for diseases such as rice blast. Probenazole is commercially available under the trade names "Oryzemate" and others.

[0012] Probenazole is registered as an agricultural chemical in Japan as one of the active ingredients of rice blast control agents and has been used as a rice blast control agent for over 40 years.

[0013]

[0014] The probenazole contained in the seed treatment agent or soil treatment agent of this embodiment may be in the form of an agriculturally and horticulturally acceptable salt thereof. Examples of such salts include salts with inorganic acids such as hydrochloric acid, sulfuric acid, and nitric acid, and salts with organic acids such as acetic acid, L-lactic acid, and citric acid.

[0015] In the seed treatment agent of this embodiment, the content of probenazole is not particularly limited, but is preferably 0.1 to 100 mass%, more preferably 0.1 to 99.9 mass%, even more preferably 0.1 to 80 mass%, and even more preferably 0.2 to 80 mass%, of the total mass of the seed treatment agent of this embodiment, as the compound represented by formula (I). In the soil treatment agent of this embodiment, the content of probenazole is not particularly limited, but is preferably 0.1 to 100 mass%, more preferably 0.1 to 99.9 mass%, even more preferably 0.1 to 80 mass%, and even more preferably 0.2 to 80 mass%, of the total mass of the soil treatment agent of this embodiment, as the compound represented by formula (I). When the seed treatment agent or soil treatment agent of this embodiment further contains an active ingredient other than probenazole, the mixing ratio of probenazole to the active ingredient other than probenazole can be varied over a wide range as appropriate.

[0016] The seed treatment agent or soil treatment agent of this embodiment may be formulated into any dosage form that a pesticide can take. The formulation may be formulated into any dosage form, such as an emulsifiable concentrate, liquid, water-soluble concentrate, water-soluble granules, emulsion, wettable powder, water-dispersible granules, flowable, suspoemulsion, oil-flowable, dust, DL dust, powder, granule, powder granule, microgranule F, tablet, oil, aerosol, smoke, or microcapsule. These various dosage forms can be produced, for example, by the method described in "Pesticide Formulation Guide" (edited by the Pesticide Science Society of Japan, Application Method Research Group, published by the Japan Plant Protection Association, 1997).

[0017] The seed treatment agent or soil treatment agent of this embodiment may further contain an additive or a carrier. The carrier can be appropriately selected from solid carriers, liquid carriers, gaseous carriers, etc. depending on the formulation.

[0018] Solid carriers can be broadly classified into powders (average particle size less than 63 μm) and granular carriers (average particle size 63 μm or more) based on particle size, and can be appropriately selected depending on the dosage form. Examples of powdered solid carriers include talc, bentonite, clay, kaolin, diatomaceous earth, white carbon (amorphous silicon, calcium silicate), calcium carbonate, ammonium sulfate, potassium sulfate, calcium sulfate, plant powders such as wood flour, starch, dextrin, etc. Examples of granular solid carriers include vermiculite, silica sand, pumice, perlite, diatomaceous earth particles, attapulgite particles, zeolite particles, bentonite particles, plant granules such as wood chips, cork, and corn cobs. Clay granules and calcium carbonate granules, which are obtained by adding a binder or the like to powdered solid carriers such as clay and calcium carbonate and granulating them, may also be used as granular solid carriers. Among these, the solid carrier is preferably at least one selected from the group consisting of talc, bentonite, clay, kaolin, diatomaceous earth, white carbon (amorphous silicon, calcium silicate), silica sand, calcium carbonate, and clay granules. The solid carrier may be a powdered carrier or a granular carrier, and may be used alone or in combination of two or more.

[0019] Examples of liquid carriers include alcohols such as ethanol, n-hexanol, polyethylene glycol, and propylene glycol; ketones such as γ-butyrolactone and cyclohexanone; aliphatic hydrocarbons such as n-hexane, normal paraffin, liquid paraffin, and naphthene; aromatic hydrocarbons such as xylene, alkylbenzene, and methylnaphthalene; ethers such as diethyl ether, dioxane, and tetrahydrofuran; esters such as fatty acid methyl esters and ethyl acetate; nitriles such as acetonitrile and isobutyronitrile; acid amides such as dimethylformamide and dimethylacetamide; vegetable oils such as soybean oil and cottonseed oil; dimethyl sulfoxide, N-alkylpyrrolidone, and water. Among these, at least one liquid carrier selected from the group consisting of propylene glycol, liquid paraffin, and water is preferred. These liquid carriers can be used alone or in combination.

[0020] Examples of gaseous carriers include liquefied propane gas (LPG), air, nitrogen, carbon dioxide, and dimethyl ether. Among these, at least one gaseous carrier selected from the group consisting of air, nitrogen, and carbon dioxide is preferred. The gaseous carriers can be used alone or in combination of two or more.

[0021] In addition to the carrier, the seed treatment agent or soil treatment agent of this embodiment may further contain, as a formulation adjuvant, at least one selected from the group consisting of surfactants that contribute to emulsification, dispersion, wetting, etc., thickeners, binders, antifoaming agents, colorants, antifungal agents, antifreezing agents, flow improvers, adhesives, and solvents, as necessary.

[0022] The surfactant may be an anionic, cationic, nonionic, or amphoteric surfactant.

[0023] Examples of the anionic surfactant include POA alkyl phenyl ether sulfate, POA styryl phenyl ether sulfate, POA alkyl ether sulfate, polyoxyethylene polyoxypropylene block polymer sulfate, lauryl sulfate (e.g., sodium salt), alkanesulfonate (e.g., sodium salt), α-olefin sulfonate, dialkyl sulfosuccinate, alkylbenzene sulfonate, naphthalenesulfonic acid formalin condensate salt (e.g., sodium salt), lignin sulfonate (e.g., sodium salt), POA alkyl phenyl ether sulfonate, POA alkyl ether sulfonate, fatty acid salt, POA alkyl phenyl ether phosphate, POA styryl phenyl ether phosphate, POA alkyl ether phosphate, polycarboxylic acid surfactant, etc. Note that "POA" represents polyoxyalkylene, and preferably represents polyoxyethylene, polyoxypropylene, or a mixture thereof. The anionic surfactant may be used alone or in combination of two or more, and among these, preferred examples include POA styryl phenyl ether sulfate, lauryl sulfate (more preferably sodium salt), alkylbenzene sulfonate (more preferably sodium salt), and lignin sulfonate (more preferably sodium salt).

[0024] Examples of the cationic surfactant include alkyltrimethylammonium chloride, methyl polyoxyalkylene alkylammonium chloride, alkyldimethylbenzylkonium chloride, etc. One of these may be used alone, or two or more may be used in combination.

[0025] Examples of the nonionic surfactant include POA styrylphenyl ether, POA sorbitan fatty acid ester, sorbitan fatty acid ester, sucrose fatty acid ester, POA castor oil, POA hydrogenated castor oil, POA alkyl ether, POA alkylphenyl ether, polyoxyethylene polyoxypropylene block polymer, POA alkylamine, POA alkylamide, POA fatty acid ester, silicone surfactant, and acetylene glycol surfactant.One of these may be used alone or in combination of two or more. Among these, POA styrylphenyl ether is preferred. POA represents one of polyoxyethylene, polyoxypropylene, or a mixture thereof.

[0026] Examples of the amphoteric surfactant include amino acid-type amphoteric surfactants such as alkyldiaminoethylglycine hydrochloride and alkylpolyaminoethylglycine hydrochloride, and betaine-type amphoteric surfactants such as alkyldimethylaminoacetic acid betaine, alkyldimethylacetic acid betaine, alkyldimethylcarboxymethyl betaine, alkyldimethylcarboxymethyleneammonium betaine, and fatty acid amidopropyldimethylamino acid betaine, but are not limited to these amphoteric surfactants. The amphoteric surfactant is preferably at least one selected from the group consisting of these amphoteric surfactants, and more preferably at least one selected from the group consisting of alkyldiaminoethylglycine hydrochloride and alkylpolyaminoethylglycine hydrochloride. The amphoteric surfactants can be used alone or in combination of two or more.

[0027] Examples of the thickener include organic thickeners such as xanthan gum, welan gum, diutan gum, guar gum, tragacanth gum, gum arabic, dextrin, carboxymethyl cellulose, polyvinyl alcohol, and water-soluble cellulose ether. Examples of the inorganic thickener include montmorillonite-based mineral fine powder, saponite-based clay mineral, and anhydrous silica fine powder, but are not limited to these. One of these may be used alone, or two or more may be used in combination.

[0028] Examples of the binder include, but are not limited to, polyvinyl alcohol, water-soluble cellulose ethers such as carboxymethyl cellulose, starch, dextrin, bentonite, etc., and one of these may be used alone or in combination of two or more. Depending on the dosage form and manufacturing method, a liquid carrier, a solid carrier, or a surfactant may play the role of a binder, so that it may not be necessary to add these binders.

[0029] Examples of the colorant include inorganic pigments such as iron oxide, titanium oxide, and Prussian blue; organic dyes such as alizarin dyes, azo dyes, and metal phthalocyanine dyes; and food dyes such as Red No. 2, Red No. 3, Red No. 40, Red No. 102, Red No. 104, Red No. 105, Red No. 106, Yellow No. 4, Yellow No. 5, Green No. 3, Blue No. 1, and Blue No. 2, and any of these may be used alone or in combination of two or more.

[0030] The antifoaming agent may be an oil-based, metal soap-based, silicone-based, or the like, and one of these may be used alone or two or more may be used in combination.

[0031] Examples of the antifreezing agent include alkylene glycols such as ethylene glycol and propylene glycol, glycerin, and urea, and one of these may be used alone or two or more may be used in combination.

[0032] Examples of the antifungal agent include potassium sorbate, p-chloro-metaxylenol, butyl p-oxybenzoate, p-hydroxybenzoic acid esters, salicylic acid derivatives, and 1,2-benzothiazolin-3-one derivatives, but are not limited to these. Furthermore, one of these may be used alone, or two or more may be used in combination.

[0033] Examples of the flow improver include, but are not limited to, white carbon (amorphous silicon, calcium silicate) and highly viscous liquids such as liquid paraffin.

[0034] Examples of the binder include, but are not limited to, water-soluble polymers such as polyethylene glycol and polyvinyl alcohol, and synthetic resin emulsions such as vinyl acetate and acrylic esters, and the like, and one of these may be used alone or in combination of two or more. Depending on the dosage form, the liquid carrier, solid carrier, surfactant, binder, etc. may also function as a binder, so that it may not be necessary to add a binder.

[0035] The formulation adjuvants are not limited to those shown here, and various adjuvants can be used as long as they can achieve the objects of the present invention. In the seed treatment agent of this embodiment, an adhesive (e.g., gypsum, polyvinyl alcohol, water-soluble cellulose ether such as carboxymethyl cellulose, starch, dextrin, alkylene glycol such as polyethylene glycol, propylene glycol, polypropylene glycol, etc.) that adheres the active ingredient to the seed surface is a preferred component, but the liquid carrier, solid carrier, binder, fixing agent, and surfactant may also serve as an adhesive, so they may not be added separately.

[0036] The above-mentioned carriers, surfactants and other formulation adjuvants may be used alone or in combination as required.

[0037] The seed treatment agent of this embodiment is an agent that protects wheat and barley from blast pathogens by spraying, smearing, dipping, or coating seeds of wheat and barley.

[0038] The soil treatment agent of this embodiment is an agent that protects wheat and barley from blast pathogens by being injected, irrigated, or mixed into soil into which wheat and barley seeds are sown.

[0039] When the seed treatment agent or soil treatment agent of this embodiment is in the form of an emulsifiable concentrate, liquid, water-soluble concentrate, water-soluble granules, emulsion, wettable powder, water-dispersible granules, flowable concentrate, suspoemulsion, or oil-flowable concentrate, it can be used in the form of a solution, suspension, or emulsion obtained by diluting it with water to a predetermined concentration.When the formulation is a dust, powder, granule, powder granule, fine granule F, or tablet, it can be used as is.When the soil treatment agent of this embodiment is in the form of an ampoule, it can be inserted into the base of a wheat or barley plant.When the soil treatment agent of this embodiment is used during seedling raising, it can be mixed with potting soil or irrigated after sowing or during seedling raising.

[0040] The application amount of the seed treatment agent or soil treatment agent of this embodiment can be changed as appropriate depending on weather conditions, formulation form, application time, application method, application location, etc. The application amount of the seed treatment agent of this embodiment is usually preferably 0.005 g to 100 g, more preferably 0.005 g to 50 g, even more preferably 0.01 g to 20 g, and even more preferably 0.01 g to 15 g, of the compound represented by formula (I) per kg of wheat or barley seeds. ２ The amount of the compound represented by formula (I) is preferably 0.001 g to 100 g, more preferably 0.005 g to 50 g, even more preferably 0.01 g to 20 g, and even more preferably 0.01 g to 5 g per 1000 mcg of soil. When the soil treatment agent of this embodiment is diluted with water and applied, the concentration of the compound represented by formula (I) is preferably 10 ppm by volume to 10,000,000 ppm by volume, more preferably 50 ppm by volume to 5,000,000 ppm by volume, even more preferably 100 ppm by volume to 2,000,000 ppm by volume, and even more preferably 100 ppm by volume to 500,000 ppm by volume, relative to the total volume of the diluted solution.

[0041] The seed treatment agent or soil treatment agent of the present embodiment may be mixed or used in combination with other active ingredients such as fungicides, insecticides (including miticides and nematicides), soil pesticides, herbicides, microbial pesticides, plant regulators, synergists, fertilizers, soil conditioners, etc., as long as the effects of the present invention are not impaired.

[0042] Specific examples of the active ingredient include those described in The Pesticide Manual, 19th Edition, 2021, published by BCPC (the British Crop Production Council) and SHIBUYA INDEX (Index of Pesticide), 17th Edition, 2014, published by SHIBUYA IDDEX RESEARCH GROUP. In the seed treatment agent or soil treatment agent of this embodiment, it is preferable that the pest control agent other than probenazole contains at least one pest control agent selected from the group consisting of insecticides and fungicides.

[0043] Examples of fungicides or insecticides that can be mixed or used in combination with the seed treatment agent or soil treatment agent of this embodiment include organophosphate ester compounds, carbamate compounds, nereistoxin derivatives, organochlorine compounds, pyrethroid compounds, benzoyl urea compounds, juvenile hormone-like compounds, molting hormone-like compounds, neonicotinoid compounds, neuronal sodium channel blockers, insecticidal macrocyclic lactones, γ-aminobutyric acid (GABA) antagonists, ryanodine receptor active compounds, insecticidal ureas, BT agents, insect pathogenic virus agents, polyether antibiotics, thiamine antagonists, and sulfonamide / folate antagonist combination agents.

[0044] Specific examples of the insecticides include acephate, dichlorvos, ethyl paranitrophenylthionobenzenephosphonate (EPN), fenitrothion, fenamifos, prothiofos, profenofos, pyraclofos, chlorpyrifos-methyl, diazinon, fosthiazate, and the like. organophosphate ester compounds such as azate and imicyafos; methomyl, thiodicarb, aldicarb, oxamyl, propoxur, carbaryl, fenobucarb, ethiofencarb, fenothiocarb, pirimicarb, and carbofuran carbamate compounds such as carbosulfan, and benfuracarb; nereistoxin derivatives such as cartap and thiocyclam; organochlorine compounds such as dicofol and tetradifon; permethrin, tefluthrin, cypermethrin, deltamethrin, cyhalothrin, pyrethroid compounds such as diflubenzuron, teflubenzuron, flufenoxuron, and chlorfluazuron;Juvenile hormone-like compounds such as methoprene; molting hormone-like compounds such as chromafenozide; imidacloprid, clothianidin, thiamethoxam, acetamiprid, nitenpyram, thiacloprid, dinotefuran, sulfoxaflor, flupyradifurone, nicotinic acetylcholine receptor agonists such as upyradifurone, dichloromezotiaz, and triflumezopyrim; diamide compounds; GABA receptor agonist compounds such as ethiprole, fipronil, pyrafluprole, pyriprole, broflanilide, and fluxametamide; pyridaben, fenpyroximate, pyrimidifen, tebufenpyrad, and tolfenpyrad, and other respiratory chain electron transport complex I inhibitors; fluopyram, cyflumetofen, cyenopyrafen, and piflubumid, and other respiratory chain electron transport complex II inhibitors; fluacrypyrim, acequinocyl, and flometoquin, and other respiratory chain electron transport complex III inhibitors;ACCase (acetyl-CoA carboxylase) inhibitor compounds such as spirodiclofen, spiromesifen, and spirotetramat; and macrolide compounds such as spinosad, avermectin, milbemycin, spinetoram, lepimectin, and emamectin benzoate;

[0045] Specific examples of the insecticide other than those mentioned above include buprofezin, hexythiazox, amitraz, chlordimeform, etoxazole, pymetrozine, bifenazate, flonicamid, chlorfenapyr, pyriproxyfen, and indoxacarb. acarb), pyridalyl, pyrifluquinazon, metaflumizone, hydramethylnon, triazamate, afidopyropene, flupirimin, lenofluthrin, chloroprallethrin, cyhalodiamide, fluensulfone (fluensulfone), fluazaindolizine, epsilon-metofluthrin, epsilon-momfluorothrin, kappa-bifenthrin, kappa-tefluthrin, fluhexafon, tioxazafen, momfluorothrin ), heptafluthrin, pyriminostrobin, cycloxaprid, isocycloseram, oxazosulfyl, cyclopyrazolor, spiropidion, acinonapyr, organometallic compounds, dinitro compounds, organosulfur compounds, urea compounds, triazine compounds, and hydrazine compounds,and microbial pesticides such as BT agents and insect pathogenic virus agents such as Bacillus firmus and Bacillus amyloliquefaciens.

[0046] The insecticide is preferably at least one selected from the group consisting of the above-mentioned insecticides. The insecticides may be used alone or in combination of two or more. The insecticide is preferably flupyrimin. The insecticide may be an agriculturally and horticulturally acceptable acid addition salt thereof.

[0047] Specific examples of the fungicide include strobilurin compounds such as azoxystrobin, kresoxim-methyl, trifloxystrobin, metominostrobin, and orysastrobin; anilinopyrimidine compounds such as mepanipyrim, pyrimethanil, and cyprodinil; triazolam, triazolam, triazolam, triazolam; Dimefon, bitertanol, triflumizole, metconazole, propiconazole, penconazole, flusilazole, myclobutanil, cyproconazole, tebuconazole, hexaconazole, pro Azole compounds such as chloraz and simeconazole; quinoxaline compounds such as quinomethionate; dithiocarbamate compounds such as maneb, zineb, mancozeb, polycarbamate, and propineb; phenylcarbamate compounds such as diethofencarb; chlorothalonil; organochlorine compounds such as halonil and quintozene; benzimidazole compounds such as benomyl, thiophanate-methyl, and carbendazim; phenylamide compounds such as metalaxyl, oxadixyl, ofurase, benalaxyl, furalaxyl, and cyprofuran;Sulfenic acid compounds such as dichlofluanid; copper compounds such as cupric hydroxide and oxyquinoline copper; isoxazole compounds such as hydroxyisoxazole; organophosphorus compounds such as fosetylaluminum and tolclofos-methyl; N-halogenothioalkyl compounds such as captan, captafol, and folpet; procymidone, iprodione dicarboximide compounds such as flutolanil, mepronil, furametpyr, thifluzamide, boscalid, and penthiopyrad; morpholine compounds such as fenpropimorph and dimethomorph; triphenyltin hydroxide; organotin compounds such as triphenyltin hydroxide and triphenyltin acetate; cyanopyrrole compounds such as fludioxonil and fenpiclonil;

[0048] In addition, other compounds include tricyclazole, pyroquilon, carpropamid, diclocymet, fenoxanil, fthalide, fluazinam, cymoxanil, triforine, pyrifenox, fenarimol, fenpropidin, and pencycuron. ycuron), ferimzone, cyazofamid, iprovalicarb, benthiavalicarb-isopropyl, iminoctadine albesilate, cyflufenamid, kasugamycin, validamycin, streptomycin, oxolinic acidacid), tebufloquine, hymexazol, flusulfamide, quinofumelin, probenazole, tiadinil, isotianil, isoprothiolane, tolprocarb, pydiflumetofen, picarbutrazox, mandestrobin, dipymetitrone, pyraziflumid, oxathiapipropi Examples of such antihistamines include oxathiapirolin, penflufen, fenpicoxamid, flusakipyroxad, isoflucipram, methyltetraprole, pyrapropoyne, pyridaclomethyl, ipflufenoquin, fluopimomide, fluindapyr, florylpicoxamid, and dichlopenthiazox.

[0049] The fungicide may be one of these alone or a combination of two or more. These fungicides may also be in the form of their agriculturally and horticulturally acceptable acid addition salts. The fungicide is preferably at least one selected from the group consisting of penthiopyrad, hymexazol, flusulfamide, quinofumelin, and tolprocarb.

[0050] Preferred compositions of the seed treatment agent or soil treatment agent of this embodiment are, for example, those listed below.

[0051] (1') A wettable powder composition containing 10 to 80% by mass of probenazole, 0.5 to 20% by mass of a wetting agent and / or dispersant (preferably a surfactant), 0 to 10% by mass of an adhesive, and 9.5 to 89.5% by mass of a powdered solid carrier.

[0052] (2') A water dispersible granule containing 0.2 to 70% by mass of probenazole, 0.6 to 30% by mass of a wetting agent and / or dispersant (preferably a surfactant), and a binder in total, and 20 to 99.2% by mass of a powdered solid carrier.

[0053] (3') A flowable composition containing 10 to 80% by mass of probenazole, 10 to 28% by mass of at least one formulation auxiliary selected from a wetting agent, a dispersant (preferably a surfactant), a thickener, an antifreeze agent, an antifungal agent, an antifoaming agent, and a fixing agent, and 10 to 80% by mass of water as a liquid carrier.

[0054] (4') A composition that is an emulsifiable concentrate containing 10 to 70% by mass of probenazole, 2 to 30% by mass of an emulsifier (preferably a surfactant), and 18 to 88% by mass of an organic solvent as a liquid carrier.

[0055] (5') A composition which is a dust or powder formulation containing 10 to 90% by weight of probenazole, 0 to 10% by weight of an adhesive, and 10 to 90% by weight of a powdered solid carrier.

[0056] (6') A composition that is a dust-granule mixture containing 0.1 to 50% by mass of probenazole, 0.2 to 10% by mass of a binder or a carrier that acts as a binder, a surfactant, or a sticking agent, and 70 to 99.7% by mass of a granular solid carrier.

[0057] (7') A composition that is an extruded granule containing 0.1 to 50% by mass of probenazole, a wetting agent and / or dispersing agent (preferably a surfactant), and a binder in a total amount of 0.5 to 30% by mass, and a powdered solid carrier in a total amount of 49.5 to 99.4% by mass.

[0058] (8') A composition that is a coated granule containing 0.1 to 60% by mass of probenazole, 0.5 to 30% by mass of a wetting agent and / or dispersant (preferably a surfactant), a powdered solid carrier, and a binder in total, and 39.5 to 99.4% by mass of a granular solid carrier.

[0059] (9') A composition that is a microcapsule containing a membrane material, 0.1 to 20% by mass of probenazole, a total of 1 to 50% by mass of an emulsifier and a dispersant (preferably a surfactant), and an antifungal agent, and 30 to 98.9% by mass of water as a liquid carrier.

[0060] Among these, the compositions (1'), (2'), (3'), (5'), (6'), (7') and (8') are more preferable.

[0061] The seed treatment agent or soil treatment agent of the second embodiment of the present invention is a combination of the seed treatment agent or soil treatment agent of the first embodiment and a control agent containing at least one selected from the group consisting of pest control agents such as fungicides and insecticides. The seed treatment agent or soil treatment agent of the first embodiment and the control agent may each be prepared individually in their respective formulations, and these may be mixed on site when used. In this embodiment, descriptions of the same components as in the first embodiment may be omitted.

[0062] In the combination of this embodiment, the seed treatment agent or soil treatment agent containing probenazole is provided as a first composition, and the control agent containing another pest control agent is provided as a second composition. In this case, the first composition and the second composition can each independently be in any formulation prepared using an appropriate carrier and / or formulation adjuvant, as with the seed treatment agent or soil treatment agent of the first embodiment described above. The combination may be provided in the form of, for example, a kit containing the first composition contained in a first container and the second composition contained in a second container.

[0063] The seed treatment agent or soil treatment agent of the present invention is applied to wheat and barley. Examples of the wheat and barley include wheat, barley, rye, and oats, preferably at least one selected from the group consisting of wheat, rye, barley, and oats, more preferably at least one selected from the group consisting of wheat, barley, and oats, even more preferably wheat or barley, and most preferably wheat.

[0064] The target disease to be controlled by the seed treatment agent or soil treatment agent of the present invention is blast disease of wheat and barley, preferably at least one selected from the group consisting of wheat blast, barley blast and oat blast, more preferably wheat blast or barley blast, and even more preferably wheat blast.

[0065] The pathogenic fungus of wheat blast, like the fungus that causes rice blast, is a fungus (imperfect fungus) classified as Pyricularia oryzae Cavara (hereinafter also referred to as "P. oryzae"), but the pathogenicity of P. oryzae to plants varies depending on the fungal group. For example, inoculation of wheat with a rice blast pathogen does not cause blast, but inoculation of barley causes blast, and inoculation of wheat blast pathogens with barley causes blast.

[0066] The seed treatment agent or soil treatment agent of the present invention can be used to control the following plant diseases in addition to controlling blast disease of wheat and barley.

[0067] Such plant diseases include, for example, viral diseases, bacterial diseases, and fungal diseases.

[0068] Examples of the viral diseases include wheat feathery dwarf disease (pathogen: wheat feathery dwarf virus (WMDV)), barley stripe mosaic disease (pathogen: barley stripe mosaic virus (BSMV)), northern wheat mosaic disease (pathogen: northern wheat mosaic virus (NCMV)), and dwarf disease (pathogen: Chinese wheat mosaic virus (CWMV); Japanese soil-borne wheat mosaic virus (JSBWMV); soil-borne wheat mosaic virus (JSBMV)). virus (SBWMV)], wheat yellow leaf disease [pathogen: wheat yellow leaf virus (WYLV)], rice stripe disease [pathogen: rice stripe virus (RSV), wheat yellow mosaic disease [pathogen: wheat yellow mosaic virus (WYMV)], black-streaked dwarf disease [pathogen: rice black-streaked dwarf virus (RBSDV)], barley yellow dwarf disease [pathogen: barley yellow dwarf virus (BYDV)], barley stripe mosaic disease [pathogen: barley stripe mosaic virus (BSMV)], barley yellow mosaic disease [pathogen: barley yellow dwarf virus (BaYMV)], and oat red leaf disease [pathogen: barley yellow dwarf virus (BYDV); wheat yellow leaf virus (WYLV)].

[0069] Examples of bacterial diseases include black node disease (pathogenic bacterium: Pseudomonas syringae pv. syringae), rye bacterial streak disease (pathogenic bacterium: Xanthomonas translucens pv. cerealis), rye halo blight (pathogenic bacterium: Pseudomonas syringae pv. coronafaciens), oat bacterial streak blight (pathogenic bacterium: Pseudomonas syringae pv. striafaciens), and oat brown leaf blight (pathogenic bacterium: Spermospora avenae).

[0070] As the filamentous fungal diseases, for example, foot rot [pathogen: Ascochyta tritici], barley leaf blotch [pathogen: Ascochyta hordei], powdery mildew [pathogen: Blumeria graminis f. sp. tritici], rye powdery mildew [pathogen: Blumeria graminis f. sp. secalis], barley foot rot [pathogen: Gaeumannomyces graminis], stripe disease [pathogen: Cephalosporium gramineum], foot rot [pathogen: Ceratobasidium cereale; Ceratobasidium gramineum], blackening disease [pathogen: Cladosporium herbarum], ergot disease [pathogen: Claviceps purpurea], black-eyed grain [pathogen: Cochliobolus sativus; Alternaria sp.], leaf spot [pathogen: Cochliobolus sativus], anthracnose [pathogen: Colletotrichum graminicola], oat leaf spot [pathogen: Drechslera sp.], oat black scald [pathogen: Dendryphion avenae-sativae], black spot [pathogen: Epicoccum nigrum], head blight [pathogen: Fusarium asiaticum; Fusarium avenaceum; Fusarium crookwellense; Fusarium culmorum; Gibberella zeae, etc.], foot rot [pathogen: Gaeumannomyces graminis], barley zonate leaf spot [pathogen: Helminthosporium zonatum], barley brown leaf spot [pathogen: Heterosporium hordei], pink snow mold [pathogen: Monographella nivalis], straw blight [pathogen: Mycosphaerella hordicola], barley leaf blotch [pathogen: Mycosphaerella tassiana], snow rot and large sclerotinia disease [pathogen: Myriosclerotinia borealis], eye spot [pathogen: Oculimacula acuformis;Oculimacula yallundae, blight [pathogen: Phaeosphaeria nodorum], angular leaf spot [pathogen: Pseudoseptoria donacis], barley angular leaf spot [pathogen: Pseudoseptoria stomaticola], barley powdery mildew [pathogen: Puccinia hordei], oat crown rust [pathogen: Puccinia coronata], black rust [pathogen: Puccinia graminis], brown rust [pathogen: Puccinia recondita], yellow rust [pathogen: Puccinia striiformis], barley soot blotch [pathogen: Pyrenophora japonica], yellow spot [pathogen: Pyrenophora tritici-repentis], barley leaf blotch [pathogen: Pyrenophora graminea], barley net blotch [pathogen: Pyrenophora teres], oat leaf blight [pathogen: Pyrenophora chaetomioides], rice blast [pathogen: Pyricularia oryzae], brown snow blight [pathogen: Pythium iwayamai; Pythium paddicum; Pythium horinouchiense; Pythium graminicola; Pythium okanoganense, etc.], yellow blight [pathogen: Pythium spp.], barley sheath blight [pathogen: Rhizoctonia solani], barley scald [pathogen: Rhynchosporium secalis f. sp. hordei], yellowing and wilting disease [pathogen: Sclerophthora macrospora], sclerotinia disease [pathogen: Sclerotium rhizodes], leaf blight [pathogen: Septoria tritici], oat brown leaf spot [pathogen: Septoria avenae], rye leaf blight [pathogen: Septoria secalis], oat sheath blight [pathogen: Thanatephorus cucumeris], common bunt [pathogen: Tilletia caries; Tilletia controversa;Tilletia laevis], snow mold (caused by Typhula incarnata; Typhula ishikariensis), ear smut (caused by Urocystis agropyri; Urocystis tritici), ear smut (caused by Ustilago tritici), barley ear smut (caused by Ustilago nuda; Ustilago avenae), and barley hard smut (caused by Ustilago hordei);

[0071] [Method for controlling blast disease in wheat and barley] A method for controlling blast disease in wheat and barley according to a third embodiment of the present invention is a method for controlling blast disease in wheat and barley, comprising treating seeds with the seed treatment agent of the first or second embodiment described above. In this embodiment, explanation of the same components as in the first or second embodiment may be omitted. The seeds are seeds of wheat and barley. Examples of the wheat and barley include wheat, barley, rye, and oats. At least one species selected from the group consisting of wheat, rye, barley, and oats is preferred, at least one species selected from the group consisting of wheat, barley, and oats is more preferred, wheat or barley is even more preferred, and wheat is most preferred.

[0072] Examples of the seed treatment method include a soaking method, a powder coating method, a smearing method, a spraying method, a pelleting method, a coating method, and a smoking method.

[0073] The soaking method is a method in which seeds are soaked in a liquid seed treatment agent according to the first or second embodiment.

[0074] The dressing method includes a dry dressing method in which the seed treatment agent of the first or second embodiment in powder form is applied to dry seeds, and a wet dressing method in which the seed treatment agent is applied to seeds that have been lightly soaked in water.

[0075] The smearing method is a method in which seeds are uniformly covered with the seed treatment agent by adding a small amount of water, an optional dye, and a predetermined amount of a wettable powder or dust of the seed treatment agent of the first or second embodiment to seeds, and stirring and mixing them.

[0076] The spraying method is a method in which the seed treatment agent of the first or second embodiment is sprayed onto the surface of seeds.

[0077] The pellet method is a method in which seeds are pelletized together with a filler into pellets of a certain size and shape, and the seed treatment agent of the first or second embodiment is mixed with the filler.

[0078] The coating method is a method of coating seeds with a film containing the seed treatment agent of the first or second embodiment.

[0079] The fumigation method is a method of disinfecting seeds with the seed treatment agent of the first or second embodiment gasified in a sealed container.

[0080] The seed treatment composition of the first or second embodiment can also be applied to germinated plants and young plants that are transplanted after germination or emergence from soil, in addition to the seeds. These plants can be protected by whole or partial immersion treatment before transplanting.

[0081] The treatment amount (effective amount) of probenazole is not particularly limited, but is preferably 0.005 g to 100 g per kg of seeds, more preferably 0.005 g to 50 g, even more preferably 0.01 g to 20 g, even more preferably 0.01 g to 15 g, and particularly preferably 0.01 g to 15 g. The effective amount to be applied to seeds (effective amount of probenazole present in the coating) is preferably within the range of 0.0002 mg / seed to 4 mg / seed, more preferably 0.0004 mg / seed to 2 mg / seed, even more preferably 0.0004 mg / seed to 0.5 mg / seed, particularly preferably 0.018 mg / seed to 0.5 mg / seed, and most preferably 0.018 mg / seed to 0.25 mg / seed. The seed treatment agent of the first or second embodiment may be applied for seed treatment directly without dilution, or may be applied for seed treatment after dilution.

[0082] A fourth embodiment of the present invention relates to a method for controlling blast disease in wheat or barley, which comprises treating soil with the soil treatment agent of the first or second embodiment of the present invention before sowing wheat or barley seeds. In this embodiment, the same components as those in the first, second, or third embodiments may not be described in detail. According to the soil treatment method of this embodiment, an excellent blast disease control effect can be achieved with a smaller amount of active ingredient than in a method of spraying an active ingredient on crops.

[0083] The soil treatment method is not particularly limited, and examples thereof include applying the soil treatment agent of the first or second embodiment to or on the soil. Examples of such soil treatment methods include incorporation treatment, spraying treatment, and band, furrow, and planting hole application treatment (including soil spraying in planting furrows and in furrow treatment). Preferred treatment methods include incorporation treatment and furrow and planting hole application treatment. For example, incorporation treatment can control wheat blast by manually or mechanically incorporation of a granular soil treatment agent into the soil. Furthermore, wheat blast can also be controlled by spraying the soil treatment agent of the first or second embodiment onto both the plants and the soil in the upper furrow of the soil surface at the time of sowing. Furthermore, applying the soil treatment agent of the first or second embodiment to the soil by irrigation is also a preferred soil treatment method.

[0084] In addition to these, examples of preferred methods for controlling blast disease in wheat and barley include a method of adding the soil treatment agent of the first or second embodiment to a solution in a solution cultivation system such as hydroponics, sand culture, NFT (Nutrient Film Technique), rock wool culture, or other solid medium cultivation; a method of applying the soil treatment agent of the first or second embodiment to a seedling box (by mixing it with bed soil, etc.); and a method of directly applying the soil treatment agent of the first or second embodiment to a solid medium such as artificial culture soil containing vermiculite or an artificial seedling mat.

[0085] When the culture carrier is soil or a culture medium other than soil (nutrient solutions for hydroponics, sand culture, NFT (Nutrient Film Technique), rock wool culture, etc.; solid media such as artificial soil and artificial seedling mats), the amount of probenazole to be treated (effective amount) is not particularly limited, but is preferably 0.1 g to 10 kg, more preferably 0.1 g to 5 kg, and even more preferably 0.5 kg to 5 kg per 10 ares of soil.

[0086] A fifth embodiment of the method for controlling blast disease in wheat or barley of the present invention comprises applying, to an area to be treated, simultaneously or separately (preferably, simultaneously) a first composition containing probenazole and a second composition containing at least one other pest control agent as the seed treatment or soil treatment agent of the second embodiment. In this embodiment, descriptions of the same components as those in the first to fourth embodiments may be omitted.

[0087] In this method, "applying simultaneously" includes mixing the first and second compositions before applying them to the area to be treated and then applying the mixture, while "applying separately" includes applying the first composition before the second composition, or applying the second composition after the first composition, without pre-mixing them.

[0088] Furthermore, in the method for controlling blast disease in wheat and barley of the present invention, techniques such as microbial pesticides, genetically modified crops, etc. Specifically, for example, microbial pesticides such as Bacillus firmus, which is a nematode control agent, and Bacillus amyloliquefaciens, which is a soil-borne disease control agent, as well as plants that produce BT (Bacillus thuringensis) toxin protein against insects and exhibit agricultural pest resistance, plants that exhibit resistance to herbicides such as glyphosate and glufosinate, and plants that have both of these properties can be used.

[0089] [Wheat and barley seeds] The wheat and barley seeds of the sixth embodiment of the present invention comprise wheat and barley seeds and probenazole adhered to at least a portion of the surface of the seeds. In addition to probenazole, other components contained in the seed treatment agent of the first or second embodiment may also be adhered to at least a portion of the surface of the wheat and barley seeds of this embodiment. The content of propenazole contained in the wheat and barley seeds of this embodiment is not particularly limited, but is preferably about the same as the treatment amount (effective amount) of probenazole per 1 mg of seed in the seed treatment agent of the first or second embodiment.

[0090] The wheat seeds of this embodiment may be, for example, wheat, barley, rye, and oats seeds, and are preferably at least one species selected from the group consisting of wheat, rye, barley, and oats, more preferably at least one species selected from the group consisting of wheat, barley, and oats, even more preferably wheat or barley, and particularly preferably wheat.

[0091] The wheat and barley seeds of this embodiment can be obtained, for example, by treating seeds with the seed treatment agent of the first or second embodiment according to the method for controlling wheat and barley blast of the third embodiment of the present invention.

[0092] The present invention will be specifically explained below with reference to formulation examples and test examples, but the present invention is not limited to these examples. The wheat seed variety tested was Shirogane Komugi (35 g to 37 g / 1000 kernels).

[0093] Formulation Example 1 (Powder) Probenazole 50% by mass Naphthalenesulfonate formalin condensate 2% by mass White carbon 1% by mass Polyvinyl alcohol 1% by mass Polyethylene glycol 3% by mass Clay balance The above ingredients were uniformly mixed and pulverized to obtain a powder for dust dressing.

[0094] Formulation Example 2 A commercially available water dispersible granule (trade name "Oryzemate Water Dispersible Granule" by MMAG Co., Ltd.) was tested. This product contains 48% by mass of probenazole.

[0095] Test Example 1 Wheat Phytotoxicity Test (Seed Dressing) Wheat seeds were individually dressed with the dressing powder prepared in Formulation Example 1 to a predetermined dose of probenazole, and sown in 3 cm polystyrene cups filled with sterilized soil. 18 days after sowing, the germination rate was calculated. The germination rate was also calculated in the same way when seeds not treated with any compound were sown (untreated). The germination rate was also calculated in the same way when wheat seeds were sown (white sample) dressed with 0.28 mg of the dressing powder prepared as in Formulation Example 1, except that probenazole was not added. The results are shown in Table 1.

[0096]

[0097] Test Example 2: Wheat phytotoxicity test (seed dressing) Wheat seeds were individually dressed with the dressing powder prepared in Formulation Example 1 so that a prescribed amount of probenazole was applied, and then sown in 3 cm polystyrene cups filled with sterilized soil. 11 days after sowing, the germination rate was calculated. The germination rate was also calculated in the same way when seeds not treated with any compound were sown (untreated). The results are shown in Table 2 below.

[0098]

[0099] [Test Example 3] Ten wheat seeds (variety: Shiroganekomugi) coated with the dressing powder prepared in Formulation Example 1 so that the probenazole treatment dose was 0.056 mg / seed were sown in a paddy rice seedling case filled with 580 mL of Prime Mix TKS-2 (manufactured by Sakata Seed Corporation) (seed dressing). Ten untreated wheat seeds were sown in the same manner (untreated). Furthermore, at the time of sowing the 10 untreated wheat seeds, 30 mg (14.4 mg as probenazole) of the commercially available water dispersible granule shown in Formulation Example 2, trade name "Oryzemate Water Dispersible Granule" (MMAG Co., Ltd., containing 48% by mass of probenazole) was irrigated (irrigation). After sowing, the seedlings were grown for a predetermined period in a growth incubator under conditions of 5,000 lux, 18°C, and 16 hours of sunshine. Two, three and four weeks after sowing, wheat seedlings were soaked in a 10,000-fold solution of Tween 20 and the wheat blast fungus (Br48 strain) was added to the soaked wheat seedlings at a conidial concentration of 1 x 10５ The plants were inoculated by spraying at a concentration of 1000 cells / mL, and after inoculation, they were kept in a moist room at 22°C in the dark for 24 hours. Five days after inoculation, the disease severity at each leaf position was determined according to the following criteria, with a disease severity of 2 or higher considered to be diseased, and the disease severity was calculated according to the formula below. Furthermore, the diseased leaf rate and control value were also calculated according to the formula below. The results are shown in Tables 3 to 5 below. Each plot was replicated three times, and the average value is shown.

[0100] Disease severity: 0: Absent (no lesions observed) 1: Pinpoint (brown spots) 2: Small (lesions less than 1.5 mm in diameter) 3: Intermediate (lesions 1.5 to 3 mm in diameter) 4: Large typical (large typical lesions 3 mm or more in diameter) 5: Shrevelin (withering and death)

[0101] Disease severity = (4 x number of leaves judged to have disease severity level 5 + 3 x number of leaves judged to have disease severity level 4 + 2 x number of leaves judged to have disease severity level 3 + 1 x number of leaves judged to have disease severity level 2) / (4 x total number of leaves surveyed) x 100 Disease leaf incidence rate (%) = (number of leaves judged to have disease severity levels 2 to 5) / (total number of leaves surveyed) x 100 Control value = (disease severity without treatment - disease severity with seed dressing or disease severity with drench) / (disease severity without treatment) x 100

[0102]

[0103]

[0104]

[0105] [Test Example 4] The conidia concentration of wheat blast fungus (Br48 strain) used for spray inoculation was 5 × 10 ４ The test was carried out under the same conditions as in Test Example 3, except that the concentration of the serotonin-containing compound was set to 1 / mL. The results are shown in Tables 6 to 8 below.

[0106]

[0107]

[0108]

[0109] Test Example 5 Ten wheat seeds (variety: Shiroganekomugi) that had been coated with the powder dressing formulation shown in Formulation Example 1 at a probenazole treatment dose of 0.004 mg / seed, 0.018 mg / seed, or 0.054 mg / seed were sown in a paddy rice seedling case filled with 580 mL of Prime Mix TKS-2 (manufactured by Sakata Seed Corporation). Ten untreated wheat seeds were also sown in the same manner (untreated). After sowing, the seedlings were grown for a predetermined period in a growth incubator under conditions of 5,000 lux, 18°C, and 16 hours of sunlight. Two, three, and four weeks after sowing, wheat seedlings were treated with wheat blast fungus (Br48 strain) containing 10,000 times the amount of Tween 20, and the conidia were grown at a concentration of 1 x 10 ５ The plants were inoculated by spraying at a concentration of 1000 cells / mL, and after inoculation, they were kept in a moist chamber at 22°C in the dark for 24 hours. Five days after inoculation, the degree of disease on each leaf position was determined and the disease severity was calculated using the method described in Test Example 3. The diseased leaf rate and control value were also calculated using the method described in Test Example 3. The results are shown in Tables 9 to 12 below. Each plot was replicated three times, and the average value is shown.

[0110]

[0111]

[0112]

[0113]

[0114] Test Example 6 Eight barley seeds (variety: Shunrai, 38-42 g / 1000 grain weight) that had been coated with the dressing powder shown in Formulation Example 1 so that the probenazole treatment dose was 0.062 mg / seed, 0.125 mg / seed, or 0.188 mg / seed were sown in a paddy rice seedling case filled with 580 mL of Prime Mix TKS-2 (manufactured by Sakata Seed Corporation). Eight untreated barley seeds were also sown in the same manner (untreated). After sowing, the seedlings were grown for a predetermined period in a growth incubator under conditions of 5,000 lux, 18°C, and 16 hours of sunlight. Four weeks after sowing, the barley seedlings were inoculated with wheat blast fungus (Br48 strain) containing 10,000 times the amount of Tween 20, at a conidia concentration of 5 x 10 ４The plants were inoculated by spraying at a concentration of 1000 cells / mL, and after inoculation, they were kept in a moist chamber at 22°C in the dark for 24 hours. Five days after inoculation, the degree of disease on each leaf position was determined and the disease severity was calculated using the method described in Test Example 3. The diseased leaf rate and control value were also calculated using the method described in Test Example 3. The results are shown in Table 13 below. Each plot was replicated three times, and the average value is shown.

[0115]

[0116] The seed treatment agent for controlling blast disease in wheat and barley, the soil treatment agent for controlling blast disease in wheat and barley, and the method for controlling blast disease in wheat and barley of the present invention can be used easily and exhibit excellent control effects against blast disease in wheat and barley.

Claims

1. A seed treatment agent for controlling blast disease in wheat and barley containing probenazole as the active ingredient.

2. The seed treatment agent for controlling blast disease in wheat or grain according to claim 1, wherein the wheat or grain is wheat or barley.

3. The seed treatment agent for controlling blast disease of wheat or barley according to claim 1, wherein the blast disease of wheat or barley is wheat blast disease or barley blast disease.

4. Use of a composition containing probenazole as an active ingredient as a seed treatment agent for controlling blast disease in wheat or grain.

5. The use according to claim 4, wherein the cereal is wheat or barley.

6. The use according to claim 4, wherein the blast disease of wheat or grain is wheat blast disease or barley blast disease.

7. Wheat seeds having probenazole attached to at least a portion of their surface.

8. The wheat seed according to claim 7, wherein the wheat is wheat or barley.

9. A method for controlling blast disease in wheat or barley, which comprises treating the seeds with the seed treatment agent for controlling blast disease in wheat or barley according to any one of claims 1 to 3 before sowing the seeds.

10. A soil treatment agent for controlling blast disease in wheat and grains, containing probenazole as the active ingredient.

11. A soil treatment agent for controlling blast disease in wheat or grains according to claim 10, wherein the wheat or grains are wheat or barley.

12. A soil treatment agent for controlling blast disease in wheat or barley according to claim 10, wherein the blast disease in wheat or barley is wheat blast disease or barley blast disease.

13. Use of a composition containing probenazole as an active ingredient as a soil treatment agent for controlling blast disease in wheat or barley.

14. The use according to claim 13, wherein the cereal is wheat or barley.

15. The use according to claim 13, wherein the blast disease of wheat or grain is wheat blast disease or barley blast disease.

16. A method for controlling blast disease in wheat or barley, comprising treating soil with the soil treatment agent for controlling blast disease in wheat or barley according to any one of claims 10 to 12 before sowing seeds of the wheat or barley.

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