Methods and systems for processing lipoaspirate

Abstract

A lipoaspirate preparation systems and corresponding methods are disclosed. In one aspect, a system and corresponding method for use with two peristaltic pumps to drive the flow of lipoaspirate and saline is disclosed. The disclosed system can be used to process adipose fat extracted by methods such as liposuction to form purified fat appropriate for reinjection into areas needing improvement (e g., cheeks, breasts, buttocks, etc.).

Description

METHODS AND SYSTEMS FOR PROCESSING LIPOASPIRATECross-Reference to Related Applications

[0001] This application claims priority to and the benefit of U.S. Provisional Patent Application Serial No. 63 / 527,961, filed on July 20, 2023, which is entirely incorporated herein by reference.Background

[0002] Transplanting adipose tissue into various regions of body, known as fat transfer or fat grafting, typically involves harvesting adipose tissue from one region of the body and reimplanting the harvested tissue into another location. The harvested tissue is often treated prior to reimplantation. While harvested adipose tissue is often used to correct contour irregularities (e.g., facial asymmetry), more recently fat grafting has been applied for larger applications, such as breast and gluteal augmentation. Although this is a very attractive approach, typical tissue survival rate from currently available systems may be poor and the overall results may not be satisfactory to patients.

[0003] The present disclosure addresses these and other shortcomings of the field of reconstructive, cosmetic, and regenerative surgery and procedures.Summary

[0004] The present disclosure relates to lipoaspirate preparation systems and corresponding methods. In one aspect, a system and corresponding method for use with two peristaltic pumps to drive the flow of lipoaspirate and saline is disclosed. The disclosed system can be used to process adipose fat extracted by methods such as liposuction to form purified fat appropriate for reinjection into areas needing improvement (e.g., cheeks, breasts, buttocks, etc.).

[0005] In one aspect, a lipoaspirate preparation system and corresponding methods are disclosed. The system and method include a lipoaspirate container configured to store lipoaspirate material, a reservoir configured to hold a solution, and a tubing system configured to forward the lipoaspirate material and the solution into a compartment. The compartment comprises a wash compartment and a pump configured to pump the solution and thelipoaspirate material into the wash compartment where the solution is mixed with the lipoaspirate material.

[0006] In another aspect, a method and corresponding system for lipoaspirate preparation are disclosed. The system and method include forwarding a solution from a solution reservoir into a wash compartment via a tubing system, forwarding lipoaspirate material from a lipoaspirate material container into the wash compartment via the tubing system, and receiving washed lipoaspirate material from the wash compartment.

[0007] In other examples, the aspects above, or any system, method, apparatus described herein can include one or more of the following features.

[0008] The system can include a plurality of pumps, at least one of the plurality of pumps being coupled to the tubing system. The at least one pump is disposed inside a housing. For example, at least two pumps can be disposed within the housing. Additionally or alternatively, at least one pump is disposed outside of the housing. The housing can be a box-shaped structure. Further, the housing can comprise a front surface

[0009] The system can also include a wash compartment. The wash compartment can be disposed inside of the housing and the tubing system is configured to use the pump to forward the lipoaspirate material and the solution to the wash compartment. Further, the lipoaspirate material and the solution can be mixed in the wash compartment.

[0010] In some implementations, the pump can be a peristaltic pump. Alternatively or additionally, the pump can comprise a roller wheel configured to pump at least one of fat tissue and saline. Further, the pump can comprise a fat tissue pump and / or a saline pump.

[0011] Further, the tubing system can comprise at least one external tube exposed outside of the housing. For example, the tubing system can comprise an external tubing system exposed outside of the housing. Further, the tubing system can comprise an internal tubing system confined inside of the housing. For example, the tubing system can comprise at least one internal tube confined within the housing. The tubing system can further include an external tubing system configured to connect to an internal tubing system disposed within the housing. Furthermore, the tubing system can be removably coupled to the pump.

[0012] In some implementations, the tubing system can include at least two layers of tubing.At least one layer of the tubing system can be configured to receive a majority of fluid flowing through the system.

[0013] The system can further include a plurality of ports configured to connect the at least one internal tube to the at least one external tube and / or a plurality of ports configured to comiect the internal tubing system to the external tubing system. The plurality of ports can comprise at least five ports, and the at least five ports can be disposed adjacent to the front surface of the housing.|0014| In some implementations, a first port can be configured to connect to the lipoaspirate container via the tubing system. The tubing system can be configured to forward a fluid flow from the lipoaspirate container to the pump through the first port. The first port can be coupled to a first valve, and the tubing system can be configured to forward the fluid flow from the lipoaspirate container to the pump through the first port and the first value. Further, the fluid flow can be configured to flow upstream from the lipoaspirate container to the fat tissue pump.

[0015] The system can further include a reservoir configured to hold a solution, such as saline. Alternatively or additionally, the solution can be any solution suitable for washing lipoaspirate material. The reservoir can be coupled to the tubing system via a second port and the tubing system can be configured to connect the reservoir to the pump via the second port. The pump can be a saline pump and the fluid flow can be configured to flow upstream from the reservoir to the saline pump.

[0016] Furthermore, the tubing system can be configured to forward the lipoaspirate material from the lipoaspirate container using the fat tissue pump, through the first port and the first valve, to the wash compartment. The first port can be disposed within a top left portion of the housing and / or within a top right portion of the housing.

[0017] The system can further include a storage and processing compartment configured to store non-enzymatic stromal vascular fraction (NESVF) material. The storage compartment can be disposed within the housing and the tubing system can be configured to connect the storage compartment via a third port. In some implementations, the system can include an interchangeable waste bag and the tubing system can be configured to connect the interchangeable waste bag via a fourth port.

[0018] Furthermore, the system can include an external storage compartment disposed outside of the housing. The tubing system can be configured to connect the external storage compartment via a fifth port. The external storage compartment can be configured to store at least one of a washed lipoaspirate and a mixture of washed lipoaspirate and NESVF.

[0019] The system can further include a first vent and a second valve coupled to the first vent. The tubing system can be configured to receive air through the first vent and the second valve. Furthermore, the system can include at least one of an emulsification and micronization compartment, an activation compartment, and a filter compartment. The fat tissue pump can be configured to forward the lipoaspirate material through the emulsification and micronization compartment. Alternatively or additionally, the tubing system can be configured to forward the lipoaspirate material, upon completion of emulsification, to the activation compartment. Furthermore, the tubing system can be configured to forward the lipoaspirate material, upon activation, via the third port to the NESVF storage and processing compartment. Alternatively or additionally, the tubing system can be configured to forward the lipoaspirate material, upon filtration, to an NESVF storage compartment. The NESVF storage compartment can be a syringe.

[0020] In some implementations, the vent can be configured to deliver air through the first vent to the tubing system and facilitate transport of the lipoaspirate material through the tubing system. A third valve can be coupled to the wash compartment, and the tubing system is configured to forward the saline from the saline reservoir through the second port using the saline pump to the wash compartment. Alternatively or additionally, the tubing system can be configured to forward the saline and the lipoaspirate material to the wash compartment concurrently. Further, the tubing system can be configured to forward saline washed lipoaspirate material to the external container via the fifth port. Furthermore, the tubing system can be configured to forward saline washed lipoaspirate material to an NESVF processing and storage compartment.

[0021] In some implementations, the lipoaspirate material are aspirated via a vacuum- assisted liposuction system. The vacuum-assisted liposuction system comprises a vacuum pump. A switch can be configured to configured to activate the vacuum pump. The vacuum- assisted liposuction system can further comprise a vacuum pressure regulator. The vacuum pump can be coupled to the vacuum pressure regulator and configured to regulate the vacuum pressure of the vacuum-assisted liposuction system. In some implementations, the vacuumpressure regulator can be controlled via at least one of a display screen or a manual interface. Further, the vacuum-assisted liposuction system can include a vacuum pressure sensor. The vacuum pressure regulator can be coupled to the vacuum pressure sensor and configured to monitor and control the pressure of the vacuum pump. In some implementations, a vacuummode selection switch can be used to place the vacuum-assisted liposuction system in a vacuum-mode. The vacuum-mode selection switch can control the operation of the vacuum pump. Alternatively or additionally, a foot pedal can be used to control the flow rate of the vacuum pump and / or the operation of the vacuum pump. The flow rate can indicate the flow rate of fluids through the vacuum pump. In some implementations, the vacuum pump can be configured for continuous operation. Further, a fitting can be used to connect the vacuum- assisted liposuction system to the lipoaspirate container. The vacuum-assisted liposuction system can further include a replaceable sterile air filter.

[0022] Other aspects and advantages of the invention can become apparent from the following drawings and description, all of which illustrate the various aspects of the invention, by way of example only.Detailed Description

[0023] The present disclosure relates to lipoaspirate preparation systems and corresponding methods. In one aspect, a system and corresponding method for use with two peristaltic pumps to drive the flow of lipoaspirate and saline is disclosed. The disclosed system can be used to process adipose fat extracted by methods such as liposuction to form purified fat appropriate for reinjection into areas needing improvement (e.g., cheeks, breasts, buttocks, etc.).

[0024] FIG. 1 illustrates an example of a preparation system 100 according to some nonlimiting embodiments disclosed herein. The preparation system 100 can comprise two apical sections with exposed channels (generally referenced herein as “tubing” or “tubes”) for removable mounting on a peristaltic pump containing a roller wheel for pumping fat tissue or saline, respectively. Generally, any suitable pump can be used, and the embodiments disclosed herein are not limited to use of a peristaltic pump. The pump can be disposed in a cartridge, and the cartridge can optionally be a disposable cartridge. The tubing or channels can comprise any suitable means of directing fluid. For example, the tubing or channels cancomprise internal flow paths fabricated into plastic and / or interior channels, and not to be tubing.

[0025] The exposed tubing sections can connect to the internal fluid flow paths of the preparation system 100, positioned independently on two separate layers (namely layer 1 and layer 2). During the operation of the preparation system 100, most of the fluid flow happens in layer 1 while layer 2 allows flow paths to cross over each other. This configuration facilitates assembly of the preparation system 100 and allows the flow through elements (e.g., filter 120) to be perpendicular to the front face of the preparation system.|0026| The front face of the preparation system comprises five ports which connect external components to the internal fluid flow paths. Port 1 130 can be disposed on a first part (e.g., top left part) of the preparation system 100 and configured to connect to a lipoaspirate container 110 to forward the fluid flow path upstream to a valve 1 135 and a fat tissue pump 140, respectively. The lipoaspirate container 110 can be a lipoaspirate container 110 of a vacuum-assisted liposuction system. For example, the lipoaspirate container 110 can be a container that is coupled to an output of a liposuction system that utilizes a vacuum-assisted aspiration system extract adipose fat from a patient.

[0027] The preparation system 100 can also be coupled to Saline Bag 150 via a second port (e.g., Port 2 155). The second port can be disposed at any suitable position in the system, for example on a top right of the system 100. The second port 155 can be configured to connect the fluid flow path upstream to a saline pump 160.

[0028] The preparation system 100 can further comprise one or more additional ports at various suitable locations. For example, the System 100 can comprise three ports on the bottom of the system. For example, Port 3 170 can be disposed on a lower left of the front face, with a female luer connector on the interface. Port 3 170 can connect to the fluid flow path positioned at the bottom of a non-enzymatic stromal vascular fraction (NESVF) Storage 115 compartment. Another portion (e.g., the bottom middle part) of the preparation system 100 can contain a Port 4 180, which provides direct access to an interchangeable waste Bag 182 via a valve 10 184 and valve 12 172. Lastly, a Port 5 190 can provide access to an external container 192, which can hold, for example, 1) washed lipoaspirate from a 200 mL wash compartment 194 or 2) a predefined mixture of washed lipoaspirate from the 200 mL wash compartment 194 and NESVF from the NESVF Storage compartment 115 (e.g., 10: 1,20: 1, respectively). The external container 192 is generally referenced in FIG. 1 as the “LGG container.” However, it should be understood that the external container can contain processed fat materials in any suitable form, for example as a mixture of NESVF and washed fat. The systems and methods disclosed herein can further include processing the fat materials prior to storage in the external container 192 in order to improve fat graft outcome.

[0029] Initial loading of the 200 mL wash compartment 194 can involve pumping lipoaspirate from the lipoaspirate container 110 through Port 1 130 and valve 1 135 using the fat tissue pump 140. Valve 3 161 is opened to allow access to the 200 mL wash compartment 194. Valve 5 162 and vent 3 163 must be open to allow for venting of the 200 mL wash compartment 194. Although disclosed as a 200 mL wash compartment, any suitable wash compartment can be used with the embodiments disclosed herein. Similarly, all other measurements are provided as examples of components that can be used with the disclosed embodiments. Any suitable sized component can be used with the embodiments disclosed herein.

[0030] Saline can be pumped from the saline Bag 150 through port 2 155 using the saline pump 160 into the 200 mL wash compartment 194. Valve 5 162 and vent 3 163 are open during this step. This configuration of the preparation system 100 allows for concurrent pumping of lipoaspirate from the lipoaspirate container 110 and saline from the saline bag 150 into the 200 mL wash compartment 194 by having separate pumps and fluid flow paths for the lipoaspirate and saline. Washed lipoaspirate can be transferred from the 200 mL wash compartment 194 to the external container 192 through Port 5 190 by opening valve 4 196, valve 17 198, valve 2 137, and vent 1 112 and utilizing the fat tissue pump 140 to pump air through valve 3.

[0031] Between the NESVF Storage 115 and the wash compartment 194 lie the NESVF production components. Washed lipoaspirate from the 200 mL wash compartment 194 is transferred to the NESVF Storage compartment 115 prior to processing. To allow for air displacement within the 200 mL Wash compartment 194 during fat removal, valve 5 192 and vent 3 163 are opened. Washed lipoaspirate is pumped from the bottom of the 200 mL Wash compartment 194 opening valve 4 196, valve 11 186, and valve 13 174 to access the Fat Tissue Pump 140. To allow for the transfer of washed lipoaspirate from the 200 mL Wash compartment 194 to the NESVF Storage compartment 115, valve 7 163, valve 14 143, and vent 2 141 are opened. After loading, all valves are closed.

[0032] NESVF production involves passing washed lipoaspirate through the emulsification & micronization compartment 121, the activation compartment 123, and the filter compartment 120, respectively. Following the initial loading step, valve 9 166, valve 13 174, and valve 15 176 are opened and the fat tissue pump 140 is used to process lipoaspirate through the emulsification and micronization compartment 121.

[0033] After emulsification, valve 6 164, valve 13 174, and valve 15 176 are opened to initiate activation 123 processing. After activation, processed lipoaspirate can either 1) be stored in the NESVF Storage compartment 115 without filtering for LGG applications only or 2) pass through the Filter 120 compartment if a NESVF injectable is required. For example, in some embodiments, the storage compartment 115 can contain NESVF without filtration.Such materials can have various applications. For example, NESVF without filtration can be used in applications such as in orthopedics patients to ensure maximum retention of stromal / regenerative components.

[0034] For filtration to occur, valve 15 176, valve 13 174 and valve 8 165 must open prior to filtration. After Filtration through the Filter Compartment 120, NESVF can be extracted manually from the NESVF Storage Compartment 115 by opening valve 15 176 and valve 16 171 as well as valve 14 143 to access vent 2 141.

[0035] For LGG preparation, mixing of washed lipoaspirate from the 200 mL Wash compartment 194 and NESVF from the NESVF Storage compartment 115 at the preferred ratio occurs by alternating between loading NESVF and washed lipoaspirate into the external container 192 at the preferred ratio.

[0036] For loading either NESVF or washed lipoaspirate into the external container 192, valve 17 198 must be open, and valve 2 137 and vent 1 112 must be open to supply the fat tissue pump 140 with air to push through the preparation system 100. To load washed lipoaspirate, valve 5 162 and vent 3 163 from the 200 mL wash compartment must be open to allow for venting, and valve 3 161 and valve 4 196 must be open. To load NESVF, valve 14 143 and vent 2 141 from the NESVF Storage compartment 115 remains open to allow for venting, and valve 15 176 and valve 11 186 is also open.

[0037] In some implementations, additional components / materials (<?.g., lyophilized powder, liquid, etc.) can be added to cleansed / optimized fat graft products of the system.

[0038] FIG. 2 is high-level block diagram of a system according to an embodiment disclosed herein. As shown, the system can comprise a cartridge that is used with the system to process lipoaspirate. The lipoaspirate can be extracted from a patient via any suitable process, for example via liposuction, such as vacuum-assisted liposuction 222. For example, a vacuum- assisted system 222 having a vacuum pump 207 including hardware to switch it on and off (e.g., solid-state relay) can be used to extract the adipose tissue from the patient. The vacuum pump 207 can be coupled with a vacuum pressure regulator 208, which can be manually adjusted or electronically controlled via touchscreen display. A vacuum pressure sensor 209 can be built into vacuum pressure regulator 208 to monitor and control the pressure of the vacuum pump 207. In some implementations, the vacuum pump 207 can be operated in response to activation of a vacuum-mode selection switch. For example, the system can further comprise a foot pedal for controlling flow rate (e.g. , the flow rate of fluids through the pump 201) as well as sensors for detecting flow / pressure. The vacuum pump 207 can run continuously or be controlled by foot the pedal or be controlled via a physical switch or a virtual switch on a touchscreen display (such as the display screen shown in FIGs. 3-10)

[0039] Although not shown in FIG. 2, the vacuum-assisted liposuction system can also the required fitting to connect vacuum tubing to the system as well as a replaceable sterile air filter.

[0040] Once extracted, the extracted lipoaspirate is stored into the lipoaspirate container 210 and forwarded via the tubing system and the fat tissue pump 240 into the cartridge (labeled in FIG. 2 generally as “liquid gold graft (LGG) cartridge”) for processing. As disclosed with reference to FIG. 1 , the cartridge can process the extracted lipoaspirate and produce filtered and / or unfiltered fat appropriate for reinjection into the patient’s body. For example, as disclosed above, the filtered lipoaspirate material can be forwarded into a syringe for reinjection into the patient’s body. Additionally or alternatively, larger quantities of lipoaspirate material (e.g., unfiltered lipoaspirate material) can be stored in an external container 292 for reinjection into the body. In some implementations, an additional pump 201 can be coupled with the external container 292 and configured to facilitate transfer of the processed lipoaspirate material into the body.

[0041] The system can further utilize the foot pedal for controlling flow rate (e.g., the flow rate of fluids through the pump 201) as well as sensors for detecting flow / pressure. The foot pedal can be disposed at any suitable location, for example, coupled to the external container292. The foot pedal can, for example, be used in controlling the flow of injection of cleansed / optimized fat materials into the patient.

[0042] The cartridge can be housed in a suitable housing. For example, the cartridge can be manufactured via injection molding, creating a single cartridge.

[0043] For example, the system can include one or more sensors configured to monitor the operations of the system. Any suitable sensor can be used. For example, an optical sensor, an infrared sensor (IR sensor), and / or a pressure sensor can be used. The pressure sensor can be used to monitor and control the pressure of the fluids flowing through the system. Optical sensors can also be used to detect and monitor the flow of fluid through the system and issue necessary warnings (e.g., generate a warning indicating presence of air bubbles in the system).

[0044] FIGs. 3-10 illustrate a system according to embodiments disclosed herein. As shown, in addition to the components disclosed with reference to FIGs. 1-2, the system can include a display screen 310 that allows controlling various functions of the system. Further, as shown in FIGs. 6-7, the system can be implemented as a mobile unit that allows easy access to the various components and convenient access for replacement and removal of various components (e.g., NESVF and lipoaspirate).

[0045] Having thus described a few particular embodiments, various alterations, modifications, and improvements will readily occur to those skilled in the art. Such alterations, modifications and improvements as are made obvious by this disclosure are intended to be part of this description though not expressly stated herein, and are intended to be within the spirit and scope of the invention. Accordingly, the foregoing description is by way of example only, and not limiting.

Claims

Claims1. A system comprising: a lipoaspirate container configured to store lipoaspirate material; a reservoir configured to hold a solution; and a tubing system configured to forward the lipoaspirate material and the solution into a compartment, the compartment comprising: a wash compartment; and a pump configured to pump the solution and the lipoaspirate material into the wash compartment where the solution is mixed with the lipoaspirate material.

2. The system of claim 1. further comprising a plurality of pumps, at least one of the plurality of pumps being coupled to the tubing system.

3. The system of any of the previous claims, wherein at least one pump is disposed inside a housing.

4. The system of any of the previous claims, wherein at least one pump is disposed outside of the housing.

5. The system of any of the previous claims, further comprising a wash compartment.

6. The system of any of the previous claims, wherein the wash compartment is disposed inside of the housing.

7. The system of any of the previous claims, wherein tubing system is configured to use the pump to forward the lipoaspirate material and the solution to the wash compartment.

8. The system of any of the previous claims, wherein the lipoaspirate material and the solution are mixed in the wash compartment.

9. The system of any of the previous claims, further comprising at least one pump disposed within the housing.

10. The system of any of the previous claims, further comprising at least two pumps disposed within the housing.

11. The system of any of the previous claims, wherein the pump comprises a peristaltic pump.

12. The system of any of the previous claims, wherein the pump comprises a roller wheel configured to pump at least one of fat tissue and saline.

13. The system of any of the previous claims, wherein the tubing system comprises at least one external tube exposed outside of the housing.

14. The system of any of the previous claims, wherein the tubing system comprises an external tubing system exposed outside of the housing.

15. The system of any of the previous claims, wherein the tubing system comprises an internal tubing system confined inside of the housing.

16. The system of any of the previous claims, wherein the tubing system comprises at least one internal tube confined within the housing.

17. The system of any of the previous claims, wherein the tubing system comprises an external tubing system configured to connect to an internal tubing system disposed within the housing.

18. The system of any of the previous claims, wherein the tubing system is removably coupled to the pump.

19. The system of any of the previous claims, wherein the tubing system comprises at least two layers of tubing.

20. The system of any of the previous claims, wherein at least one layer of the tubing system is configured to receive a majority of fluid flowing through the system.

21. The system of any of the previous claims, wherein the housing comprises a box-shaped structure.

22. The system of any of the previous claims, wherein the housing comprises a front surface.

23. The system of any of the previous claims, further comprising a plurality of ports configured to connect the at least one internal tube to the at least one external tube.

24. The system of any of the previous claims, further comprising a plurality of ports configured to connect the internal tubing system to the external tubing system.

25. The system of any of the previous claims, wherein the plurality of ports comprises at least five ports.

26. The system of any of the previous claims, wherein the at least five ports are disposed adjacent to the front surface of the housing.

27. The system of any of the previous claims, further comprising a first port configured to connect to the lipoaspirate container via the tubing system.

28. The system of any of the previous claims, wherein the tubing system is configured to forward a fluid flow from the lipoaspirate container to the pump through the first port.

29. The system of any of the previous claims, wherein the pump comprises a fat tissue pump.

30. The system of any of the previous claims, wherein the first port is coupled to a first valve.

31. The system of any of the previous claims, wherein the tubing system is configured to forward the fluid flow from the lipoaspirate container to the pump through the first port and the first value.

32. The system of any of the previous claims, wherein the fluid flow is configured to flow upstream from the lipoaspirate container to the fat tissue pump.

33. The system of any of the previous claims, further comprising a reservoir configured to hold a solution.

34. The system of any of the previous claims, wherein the solution comprises saline.

35. The system of any of the previous claims, wherein the solution comprises a solution suitable for washing lipoaspirate material.

36. The system of any of the previous claims, wherein the reservoir is coupled to the tubing system via a second port.

37. The system of any of the previous claims, wherein the tubing system is configured to connect the reservoir to the pump via the second port.

38. The system of any of the previous claims, wherein the pump comprises a saline pump.

39. The system of any of the previous claims, wherein the fluid flow is configured to flow upstream from the reservoir to the saline pump.

40. The system of any of the previous claims, wherein the tubing system is configured to forward the lipoaspirate material from the lipoaspirate container using the fat tissue pump, through the first port and the first valve, to the wash compartment.

41. The system of any of the previous claims, wherein the first port is disposed within a top left portion of the housing.

42. The system of any of the previous claims, wherein the first port is disposed within a top right portion of the housing.

43. The system of any of the previous claims, further comprising a storage and processing compartment configured to store non-enzy matic stromal vascular fraction (NESVF) material.

44. The system of any of the previous claims, wherein the storage compartment is disposed within the housing.

45. The system of any of the previous claims, wherein the tubing system is configured to connect the storage compartment via a third port.

46. The system of any of the previous claims, further comprising an interchangeable waste bag.

47. The system of any of the previous claims, wherein the tubing system is configured to connect the interchangeable waste bag via a fourth port.

48. The system of any of the previous claims, further comprising an external storage compartment disposed outside of the housing.

49. The system of any of the previous claims, wherein the tubing system is configured to connect the external storage compartment via a fifth port.

50. The system of any of the previous claims, wherein the external storage compartment is configured to store at least one of a washed lipoaspirate and a mixture of washed lipoaspirate and NESVF.

51. The system of any of the previous claims, further comprising a first vent.

52. The system of any of the previous claims, further comprising a second valve coupled to the first vent.

53. The system of any of the previous claims, wherein the tubing system is configured to receive air through the first vent and the second valve.

54. The system of any of the previous claims, further comprising an emulsification an micronization compartment.

55. The system of any of the previous claims, further comprising an activation compartment.

56. The system of any of the previous claims, further comprising a filter compartment.

57. The system of any of the previous claims, wherein the fat tissue pump is configured to forward the lipoaspirate material through the emulsification and micronization compartment.

58. The system of any of the previous claims, wherein the tubing system is configured to forward the lipoaspirate material, upon completion of emulsification, to the activation compartment.

59. The system of any of the previous claims, wherein the tubing system is configured to forward the lipoaspirate material, upon activation, via the third port to the NESVF storage and processing compartment.

60. The system of any of the previous claims, wherein the tubing system is configured to forward the lipoaspirate material, upon filtration, to an NESVF storage compartment.

61. The system of any of the previous claims, wherein NESVF storage compartment comprises a syringe.

62. The system of any of the previous claims, wherein the vent is configured to deliver air through the first vent to the tubing system and facilitate transport of the lipoaspirate material through the tubing system.

63. The system of any of the previous claims, further comprising a third valve coupled to the wash compartment.

64. The system of any of the previous claims, wherein the tubing system is configured to forward the saline from the saline reservoir through the second port using the saline pump to the wash compartment.

65. The system of any of the previous claims, wherein the tubing system is configured to forward the saline and the lipoaspirate material to the wash compartment concurrently.

66. The system of any of the previous claims, wherein the tubing system is configured to forward saline washed lipoaspirate material to the external container via the fifth port.

67. The system of any of the previous claims, wherein the tubing system is configured to forward saline washed lipoaspirate material to an NESVF processing and storage compartment.

68. The system of any of the previous claims, further comprising aspirating the lipoaspirate material via a vacuum-assisted liposuction system.

69. The system of any of the previous claims, wherein the vacuum-assisted liposuction system comprises a vacuum pump.

70. The system of any of the previous claims, further comprising a switch configured to configured to activate the vacuum pump.

71. The system of any of the previous claims, further comprising a vacuum pressure regulator.

72. The system of any of the previous claims, wherein the vacuum pump is coupled to the vacuum pressure regulator.

73. The system of any of the previous claims, wherein the vacuum pressure regulator is configured to regulate the vacuum pressure of the vacuum-assisted liposuction system.

74. The system of any of the previous claims, wherein the vacuum pressure regulator is controlled via a display screen.

75. The system of any of the previous claims, wherein the vacuum pressure regulator is controlled via a manual interface.

76. The system of any of the previous claims, further comprising a vacuum pressure sensor.

77. The system of any of the previous claims, wherein the vacuum pressure regulator is coupled to the vacuum pressure sensor.

78. The system of any of the previous claims, wherein the vacuum pressure sensor is configured to monitor and control the pressure of the vacuum pump.

79. The system of any of the previous claims, further comprising a vacuum-mode selection switch.

80. The system of any of the previous claims, wherein the vacuum-mode selection switch is configured to control the operation of the vacuum pump.

81. The system of any of the previous claims, further comprising a foot pedal configured to control the flow rate of the vacuum pump.

82. The system of any of the previous claims, wherein the flow rate comprises the flow rate of fluids through the vacuum pump.

83. The system of any of the previous claims, wherein the vacuum pump is configured for continuous operation.

84. The system of any of the previous claims, wherein the foot pedal is configured to control operation of the vacuum pump.

85. The system of any of the previous claims, further comprising a fitting configured to connect the vacuum-assisted liposuction system to the lipoaspirate container.

86. The system of any of the previous claims, further comprising a replaceable sterile air filter.Y187. A method for using the system disclosed in claims 1-86 to process the lipoaspirate material.

88. A method comprising: forwarding a solution from a solution reservoir into a wash compartment via a tubing system: forwarding lipoaspirate material from a lipoaspirate material container into the wash compartment via the tubing system; and receiving washed lipoaspirate material from the wash compartment.

89. The method of claim 88. further comprising using the system disclosed in claims 1-86 to produce washed lipoaspirate material.

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