An Anti-inflammatory hair oil composition for the treatment of alopecia areta and process of preparing the same

Abstract

This invention relates to an Anti-inflammatory hair oil composition for the treatment of Alopecia areta and other related hair problems and in particular, this invention relates to the Anti-inflammatory hair oil composition wherein the hair oil was prepared from extract of Curcuma caesia that can be aqueous or non-aqueous, infused with a non-reactive carrier oil. This invention also relates to the process of preparing the Anti- inflammatory hair oil composition. More particularly, this present invention relates to the Anti-inflammatory hair oil composition has the advantages of increasing the efficiency and cost is low and prevent the hair growth problems and skin related problems.

Description

AN ANTI-INFLAMMATORY HAIR OIL COMPOSITION FOR THE TREATMENT OF ALOPECIA ARETA AND PROCESS OF PREPARING THESAMECROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This application claims priority from Indian Patent Application Number 202331052741, entitled “AN ANTI-INFLAMMATORY HAIR OIL COMPOSITION FOR THE TREATMENT OF ALOPECIA ARETA AND PROCESS OF PREPARING THE SAME”, filed on August 05, 2023, which application is hereby incorporated herein by reference in its entirety.FIELD OF THE INVENTION

[0002] The invention generally relates to the field of hair oil and more particularly it relates to anti-inflammatory hair oil composition for treatment of Alopecia areta and other hair related problems.BACKGROUND OF THE INVENTION

[0003] The following background information may present examples of specific aspects of the prior art (e.g., without limitation, approaches, facts, or common wisdom) that, while expected to be helpful to provide additional aspects of the prior art, is not to be construed as limiting the present invention, or any embodiments thereof, to anything stated or implied therein or inferred thereupon.

[0004] Having healthy hairs and protecting healthy hairs is significant. Achieving healthy hair and prevent hair loss for any reason whatsoever affects human psychology negatively and as a consequence it affects life quality. There are numerous works and products in order to prevent hair loss and to grow lost hairs. In this subject, harmful dermatological chemical mixtures exist in the market and in the developed technologies and some nuisance processes for peoples. Further, the chemical compounds consisting of various chemicals in hair oil composition has some adverseeffect of the skin, scalp and the like.

[0005] Generally, the method of development of a natural and organic technology which is highly efficient and fast acting oil to manage Alopecia and other hair related problems is difficult.

[0006] Numerous attempts have been made and several prior arts are known for variety of composition of herbal hair oils for growth of hair. Even though these innovations may be suitable for the specific purposes to which they address, however, they would not be as suitable for the purposes of the present invention.

[0007] For example, Japanese Pat. No. JP2020070241A to Nishinokawa Takaya discloses a a black turmeric-containing composition that is extremely effective for hair restoration, hair growth, and scalp care or has anti-glycation action blended with a proper rate of vitamin C.

[0008] For example, Indian patent IN202321027770A to Dinkar Narayan Kadam et al. discloses an invention relates to herbal composition comprising Curcuma caesia Roxb (black turmeric) with or without other plants where the formulation is useful for medicinal preparation for various diseases. For example, German patent DE202023103946U1 to Pradhan Debasish et al. discloses a cosmeceutical herbal antiaging and anti-wrinkle nano-formulation that includes black turmeric plant extract in an amount of 0.65%, grape seed extract in an amount of 20%, nanoparticles of chitosan and mesoporous silica in an amount of 15% and the nanosystem of chitosan and mesoporous silica nanoparticles comprises ascorbic acid in a ratio of 0.06 mg to 0.07 mg, d-alphatocopherol in a ratio of 0.06 mg to 0.07 mg, vitamin B complex in an amount of 0.0065 mg, anhydrous ethanol in an amount of 5 ml and mesoporous silica nanoparticles in an amount of 0.25 g.

[0009] For example, Chinese patent CN110755593A T1 to Wang Ye et al. discloses a traditional Chinese medicine composition for promoting hair growth comprises the following bulk drugs in parts by weight: 3 parts to 10 parts of rhizoma drynariae, 3 parts to 10 parts of cacumen platycladi, 2 parts to 5 parts of rhizoma zingiberis recens, 2 parts to 5 parts of radix notoginseng, 1 part to 3 parts of walnutskin, 1 part to 3 parts of Curcuma caesia Roxb., 1 part to 3 parts of seed of champion bauhinia, 1 part to 3 parts of borneol camphor leaves and 1 part to 3 parts of Hongan camphor leaves.

[0010] For example, Japanese patent JPW02013100111A1 to Shimada Tsutomu discloses a sirtuin activator comprising black turmeric or black turmeric extract, which is easily available, highly safe, and has been eaten since ancient times as an active ingredient.

[0011] For example, German patent application JP2019199406A to Nishinokawa TakayA discloses a composition for improving male function comprises a black turmeric and any of zinc, arginine, citrulline, vitamin C, and vitamin E at a prescribed blending ratio, and in addition, a proper dosage for one day with the black turmeric component as reference, is determined.

[0012] Although various embodiments of the invention may have been motivated by various deficiencies with the prior art, which may be discussed or alluded to in one or more places in the specification, the embodiments of the invention do not necessarily address the most active compounds responsible for hair and skin care, further its perfect blended composition with carrier oil and other important ingredients to provide better therapeutic efficiency. In other words, different embodiments of the invention may address different deficiencies that may be discussed in the specification. Some embodiments may only partially address some deficiencies or just one deficiency that may be discussed in the specification, and some embodiments may not address any of these deficiencies. Thus, there is a need to prepare a novel hair oil composition that is anti-androgenic, anti-inflammatory, anti-oxidant and anti-microbial and can be used as a hair care and skin care composition.SUMMARY OF THE INVENTION

[0013] The present invention relates to an anti-androgenic, anti-inflammatory, anti-oxidant and anti-microbial hair oil composition for the treatment of Androgenetic Alopecia, Alopecia Areta and other hair related problems, wherein the hair oil wasprepared using extract of Curcuma Caesia along with other ingredients at desired proportion that can be aqueous or non-aqueous, infused with a non-reactive carrier oil.

[0014] Generally, harmful dermatological chemical mixtures exist in the market to manage Alopecia and other hair related problems. Therefore, it is an objective of the present invention is to provide a composition comprising natural and organic ingredients that is highly efficient and fast acting oil to manage Alopecia and other hair related problems.

[0015] Another objective of the present invention is to prepare anti-inflammatory hair oil composition that has high efficiency to prevent or stop skin related problems particularly skin damage on scalp due to harmful lights / radiation, exposure to extreme weather conditions and pollutants, specifically water pollutants including heavy metals, chemical and arsenic.

[0016] Another objective of the present invention is to provide a hair oil composition that has anti-androgenic, anti-inflammatory, anti-oxidant and antimicrobial properties, it improves the hair growth by maintaining the connective tissues of hair follicle.

[0017] Another objective of the present invention is to provide a hair oil composition that is easy to manufacture, easy to use and low cost.

[0018] Another objective of the present invention is to provide a hair oil composition wherein the ratio of coconut oil is 80-90 % w / w.

[0019] Another objective of the present invention is to provide a hair oil composition wherein the ratio of extract of Black turmeric (Curcuma caesia) is 10-20 % w / w.

[0020] Another objective of the present invention is to provide a composition consisting compound extracted from Curcuma caesia at a specific temperature and extraction method that can be aqueous or non-aqueous, infused with a non-reactive carrier oil that can be applied topically to inhibit DHT (dihydrotestosterone) from binding with the androgen receptors present in our sebaceous gland and hair follicle.

[0021] Another objective of the invention is to provide a formulation can also beused to inhibit 5-alpha reductase from converting testosterone to DHT due to the presence of germacrone.

[0022] Another objective of the present invention is to provide an oil composition that comprises Ar-turmerone that exerts anti-inflammatory activities in HaCat Keratinocytes by inactivating the Hedgehog pathway. Camphor and curcumene also aids in the management of anti-inflammatory condition.

[0023] Another objective of the present invention is to provide an oil composition that has high anti-inflammatory, antioxidant and anti-microbial properties, it improves the hair growth by maintaining the connective tissues of hair follicle.

[0024] Another objective of the present invention is to provide an oil composition that treats problems of fungal growth, hair loss, scalp infection are the root cause of many hair issues.

[0025] These and other objectives, advantages and aspects of the invention will become apparent from the following detailed description, taken in conjunction with the accompanying, by way of example, the features in accordance with embodiments of the invention. The summary is not intended to limit the scope of the invention, which is defined solely by the claims attached hereto.DETAILED DESCRIPTION OF THE INVENTION

[0026] The following detailed description is merely exemplary in nature and is not intended to limit the described embodiments or the application and uses of the described embodiments. As used herein, the word “exemplary” or “illustrative” means “serving as an example, instance, or illustration.” Any implementation described herein as “exemplary” or “illustrative” is not necessarily to be construed as preferred or advantageous over other implementations. All of the implementations described below are exemplary implementations provided to enable persons skilled in the art to make or use the embodiments of the disclosure and are not intended to limit the scope of the disclosure, which is defined by the claims. Furthermore, there is no intention to be bound by any expressed or implied theory presented in the preceding technical field,background, brief summary or the following detailed description. It is also to be understood that the specific processes described in the following specification, are simply exemplary embodiments of the inventive concepts defined in the appended claims.

[0027] According to several embodiments of the present invention an anti- androgenic, anti-inflammatory, anti-oxidant and anti-microbial hair oil composition comprises selective compound extracted from Curcuma Caesia at a specific temperature and extraction method, wherein the extracted selective compounds are infused with a non-reactive carrier oil that can be applied topically to inhibit DHT (dihydrotestosterone) from binding with the androgen receptors present in our sebaceous gland and hair follicle. This formulation can also be used to inhibit 5-alpha reductase from converting testosterone to DHT due to the presence of germacrone. The hair oil composition further has anti-inflammatory activities in HaCat Keratinocytes by inactivating the Hedgehog pathway. Camphor also aids in the management of antiinflammatory condition.

[0028] According to an aspect of the present invention, a hair oil composition comprises a first solution comprising active ingredients extracted from Curcuma Caesia in the range of 85-97%, Stigmasterol in the range of 0.3% to 1.5% and 0- sitosterol in the range of 0.3% to 1.5%, wherein the active ingredients of Curcuma Caesia are Ar-turmerone, Germacrone, Epicurzerenone, Curzerenone, Eucalyptol, and Camphor; and a second solution comprising carrier oil in the range of 99% to 99.8%, Vitamin A in the range of 0.1% to 0.5% and Quercetin in the range of 0.1% to 0.5%, wherein, 1- 20 w / w parts of the first solution is infused with 80 - 99 w / w parts of the second solution.

[0029] According to another aspect of the present invention, the selective compounds of Curcuma Caesia comprises Ar-turmerone in the range of 12%-44%, Germacrone in the range of 10.3%- 12%, Epicurzerenone in the range of 12%-15%, Curzerenone in the range of 18%-25.5%, Eucalyptol in the range of 10%-12%, and Camphor in the range of 3%-7.6%.

[0030] According to another aspect of the present invention, the carrier oil is virgin coconut oil.

[0031] According to another aspect of the present invention, the hair oil composition is anti-inflammatory, anti-androgenic, anti-bacterial, anti-fungal and anti- oxidative thereby allowing effective use when applied topically to treat and prevent hair related problems including Alopecia areta and Androgenetic alopecia and skin related problems including acne, atopic dermatitis, hives, contact dermatitis and heat rashes.

[0032] According to another aspect of the present invention, the hair oil composition is configured to be used as a hair care and skin care product in the form including oil, shampoo, gel, serum, lotion, cream using 0.01 to 50 percent concentration of the hair oil composition.

[0033] According to another aspect of the present invention, a method of preparation of a hair oil composition, wherein the method comprises cleaning and cutting fresh black turmeric (Curcuma Caesia) rhizome into slices of thickness 6mm or less; then, air drying the slices at room temperature for 5 to 9 days till the moisture content decreases to less than 20 percent; pulverizing the dried slices into fine powder form; then, conducting steam distillation of the powder at a temperature of 65 to 75 degrees centigrade to extract active compounds of Curcuma Caesia including Ar- turmerone, Germacrone, Epicurzerenone, Curzerenone, Eucalyptol, and Camphor in the range of 85-97% of the steam distillation extraction solution; then, mixing Stigmasterol in the range of 0.1 % to 1.5% and 0-sitosterol in the range of 0.1 % to 1.5% with the steam distillation extraction solution in dark at room temperature to form a first solution; thereafter, in the next step, a second solution is prepared by adding 0.1% to 0.5% Vitamin A and 0.1% to 0.5% Quercetin in 99% virgin coconut oil; then, infusing 1 to 20 parts of the first solution with 80 to 99 parts of the second solution at a temperature 30-60°C with constant stirring at 600-800 rpm for 2-6 hours then, allowed to cool down in dark at room temperature to make a third solution; then, mixing the third solution with desired amount of anti-caking agents, thickening agents,natural stabilizer, natural antifoaming agent, preservative, pH maintaining agent and anti-rancidity agent to form a fourth solution; the, centrifuging the fourth solution at 4000-5000 rpm and finally, filtering to remove impurities to form the hair oil composition.

[0034] According to another aspect of the present invention, the anti-caking agents and thickening agents maintain the viscosity of the hair oil composition in the range of 30-38 cP.

[0035] According to another aspect of the present invention, the anti-rancidity agent in the range of 0.01%-0.5% is added to avoid photocatalytic oxidation of the hair oil composition.

[0036] According to another aspect of the present invention, the Stigmasterol and the P-sitosterol are allowed to completely soluble with the steam distillation extraction solution using sonication at 35 to 45 degrees centigrade for 1-3 hours.

[0037] According to another aspect of the present invention, the pH of the hair oil composition is maintained between 4.5 and 5.

[0038] According to an embodiment of the present invention, the hair oil composition comprises a first solution having active compounds extracted from Curcuma Caesia using steam distillation at temperature 65-75 degree centigrade, the active compounds constitute 85-97 percent of the entire extracted solution wherein , the active compounds are Ar-turmerone in the range of 12%-44%, Germacrone in the range of 10.3%- 12%, Epicurzerenone in the range of 12%-15%, Curzerenone in the range of 18%-25.5%, Eucalyptol in the range of 10%-12%, and Camphor in the range of 3%-7.6%.

[0039] According to another embodiment of the present invention, the first solution of the hair oil composition further comprises Stigmasterol in the range of 0.3% to 1.5%, P-sitosterol in the range of 0.3% to 1.5%, along with the selective extracted compounds of Curcuma Caesia.

[0040] According to another embodiment of the present invention, Epicurzerenone and Germacrone both exhibit inhibitory activity on 5 alpha reductase or blocksandrogen receptors preventing DHT from attaching to those receptors. Thus, minimizing the effect of DHT on hair follicle to prevent and treat Androgenetic Alopecia.

[0041] According to another embodiment of the present invention, the major volatile compounds infused in the hair oil composition are Camphor, Ar-turmerone, Cur cumene etc., wherein Ar- turmerone exerts anti-inflammatory activities in HaCat Keratinocytes by inactivating the Hedgehog pathway. Further, Camphor and Curcumene also aids in the management of anti-inflammatory condition. As alopecia areta is an auto immune diseases, this means that the immune system mistakenly attacks hair follicles causing inflammation. This inflammation causes the hair to fall out. It can be treated by stopping the inflammation. So, the high anti-inflammatory properties of the Curcuma caesia extract shows excellent effectiveness in treating alopecia areta.

[0042] According to another embodiment of the present invention, Germacrone, Epicurzerenone and Ar-turmerone act as the major compounds for a DHT / 5 Alpha reductase blocker.

[0043] According to another embodiment of the present invention, the hair oil composition a second solution comprising carrier oil in the range of 99% to 99.8%, Vitamin A in the range of 0.1% to 0.5% and Quercetin in the range of 0.1% to 0.5%.

[0044] According to another embodiment of the present invention, the hair oil composition comprises 1-20 parts of the first solution and 80-99 parts of the second solution of the hair oil composition.

[0045] According to another embodiment of the present invention, a method of preparation of the first solution of the hair oil composition comprises the step of cleaning and cutting fresh black turmeric (Curcuma Caesia) rhizome into slices of thickness 6mm or less, then the slices are cleaned and air dried at room temperature for 5 to 9 days till the moisture content decreases to less than 20 percent. Then, pulverizing the dried slices of the Curcuma Caesia into fine powder form. Then the powder is used to extract selective compounds such as Ar-turmerone, Germacrone, Epicurzerenone,Curzerenone, Eucalyptol, and Camphor, wherein the extraction is carried out using steam distillation process at a temperature of 65 to 75 degrees centigrade. The next step comprises, adding Stigmasterol in the range of 0.1% to 1.5% and P-sitosterol in the range of 0.1% to 1.5% with the steam distillation extraction solution to form the mixture solution. Then the mixture solution is sonicated for 1-3 hours at 35 to 45 degrees centigrade for the completely solubility of the added ingredients Stigmasterol and P-sitosterol in the steam distillation extraction solution to form soluble mixture solution. Thereafter, the soluble mixture solution is allowed to sit in dark at room temperature to avoid photocatalysis, so as to form the first solution.

[0046] According to another embodiment of the present invention, a method of preparation of the second solution of the hair oil composition comprises the step of adding 0.1% to 0.5% Vitamin A and 0.1% to 0.5% Quercetin in virgin coconut oil till they get completely soluble to form the second solution.

[0047] According to another embodiment of the present invention, a method of infusion of 1 to 1-20 parts of the first solution with 80 to 99 parts of the second solution at a temperature 30-60°C with constant stirring at 600-800 rpm for 2-6 hours to make a third solution. Then the third solution is allowed to cool down at room temperature in a dark room to avoid photocatalytic reaction.

[0048] According to another embodiment of the present invention, the third solution is then mixed with:• anti-caking agents and thickening agents are used to maintain the viscosity of the hair oil composition in the range of 30-38 cP;• a natural stabilizer such as selected from without limitation, Vitamin E, Argan oil, rosemary oil, grapeseed fruit oil etc. or any combination thereof are added at a range of 0.01% to 0.5% to prevent degradation while maintaining quality of the hair oil composition.a natural antifoaming agent and preservative in a range of 0.01% to 2% are added to ensure quality and efficacy of the hair oil composition in a range of 0.01% to 2%;• a pH maintaining agent of 0. Img in 100 ml of the hair oil composition is added to bring the pH of the composition between 4.5-5; and• an anti-rancidity agent in the range of 0.01%-0.5% to avoid photocatalytic oxidation of the composition; wherein the additives / agents are added without limitation to any sequence to form a fourth solution.

[0049] According to another embodiment of the present invention, the fourth solution is then allowed to centrifuge at 5000 rpm to separate any impurities to conduct a fluent filtration to remove any solid particles or impurities to form the hair oil composition of the present invention, which is stored in a light impervious sterile container to preserve the potency of the infused oil for effective use when applied topically to treat and prevent Alopecia areta, Androgenetic alopecia and other related hair problems and skin related problems.

[0050] According to another embodiment of the present invention, the hair oil composition is used to treat and / or prevent problems related to hair growth, scalp nourishment, dandruff, fungus growth on scalp and hair, dry hair, telogen effluvium, dry scalp, damaged hair, hair breakage, non-scarring hair loss, split ends of hair, hair shaft breakage, scalp irritation, scalp acne and the like.

[0051] According to another embodiment of the present invention, the hair oil composition of the present invention has high anti-oxidant and anti-inflammatory properties, thus it is used to treat and / or prevent skin related problems such as acne, atopic dermatitis, hives, contact dermatitis, heat rashes and the like.

[0052] The biological resources such as Curcuma Caesia (Black Turmeric) is used and are collected from the northeastern region of India at the time of research and formulation of the invention, further, the carrier oil containing virgin coconut oil is procured from the local market and plentily available in all region of India. The carrieroil is any suitable oil without limitation to virgin coconut oil.

[0053] The efficacy study of the hair oil composition of the present invention is carried out in comparison with other hair oil available in the market and it is found that the hair oil composition of the present invention is much more efficient.

[0054] TEST REPORT SUMMARY:• The study was conducted in accordance to the ISO and previous reported literature to evaluate the hair growth of test sample.• The test sample was administered through the topical route, and 0.1 ml was applied on the dorsal region on rats for up to 20 days.• The rats were evaluated for hair growth and skin erythema on days 1, 5, 10, 15 and 20.• The tests are conducted at room temperature.• At the end of study, rats were sacrificed and treated skins were isolated for histopathological changes.

[0055] Purpose of the Study:The test article identified below was evaluated for skin reactiveness and hair growth through topical administration in rats.

[0056] Testing Guidelines:The study was conducted based on the international organization for Standardization, and previous reported literatures.

[0057] TEST METHODSPreparation of Animals:Rats were randomly selected, weighed, and marked individually for the identification. A total of five groups were formed and containing six rats in each group. The animals were kept in the cages for five days prior to dosing to allow for acclimatization to the laboratory conditions.Preparation of test drug:Test drug was directly applied on the dorsal region after the removal of hair.General observation:Photographs were taken at day 1, 5, 10, 15, and 20. The hair length was measured at the day 1, 5, 10, 15, and 20 of all treated rats.

[0058] RESULTS & DISCUSSIONMortality: No mortality was observed during the experimentation.

[0059] General Observations:The hair length of rats was measured using Digital Vernier Callipers. A mean of 20 hairs was taken from 1 cm2 of area:

[0060] It is identified from the above test results and the observations that the Hair oil composition of the present invention has more therapeutic efficacy than the other hair oil compositions and, it is also observed that the there is no adverse reaction on the skin when applied topically to the desired / affected area.

[0061] Because many modifications, variations, and changes in detail can be made to the described preferred embodiments of the invention, it is intended that all matters in the foregoing description and shown in the accompanying drawings be interpreted as illustrative and not in a limiting sense. Thus, the scope of the invention should be determined by the appended claims and their legal equivalence.

Claims

CLAIMSWe Claim:

1. A hair oil composition comprises: a) a first solution comprising active ingredients extracted from Curcuma Caesia in the range of 85-97%, Stigmasterol in the range of 0.3% to 1.5% and P-sitosterol in the range of 0.3% to 1.5%, wherein the active ingredients are Ar-turmerone, Germacrone, Epicurzerenone, Curzerenone, Eucalyptol, and Camphor; and b) a second solution comprising carrier oil in the range of 99% to 99.8%, Vitamin A in the range of 0.1% to 0.5% and Quercetin in the range of 0.1% to 0.5%, wherein, 1- 20 w / w parts of the first solution is infused with 80 - 99 w / w parts of the second solution.

2. The hair oil composition as claimed in claim 1, wherein the selective compounds of Curcuma Caesia comprises Ar-turmerone in the range of 12%- 44%, Germacrone in the range of 10.3%- 12%, Epicurzerenone in the range of 12%- 15%, Curzerenone in the range of 18%-25.5%, Eucalyptol in the range of 10%-12%, and Camphor in the range of 3%-7.6%.

3. The hair oil composition as claimed in claim 1, wherein the carrier oil is virgin coconut oil.

4. The hair oil composition as claimed in claim 1 , wherein the hair oil composition is anti-inflammatory, anti-androgenic, anti-bacterial, anti-fungal and anti- oxidative thereby allowing effective use when applied topically to treat and prevent hair related problems including Alopecia areta and Androgenetic alopecia and skin related problems including acne, atopic dermatitis, hives, contact dermatitis and heat rashes.

5. The hair oil composition as claimed in claim 1 , wherein the hair oil compositionis configured to be used as a hair care and skin care product in the form including oil, shampoo, gel, serum, lotion, cream using 0.01 to 50 percent concentration of the hair oil composition.

6. A method of preparation of a hair oil composition, wherein the method comprises: a) cleaning and cutting fresh black turmeric (Curcuma Caesia) rhizome into slices of thickness 6mm or less; b) air drying the slices at room temperature for 5 to 9 days till the moisture content decreases to less than 20 percent; c) pulverizing the dried slices into fine powder form; d) conducting steam distillation of the powder at a temperature of 65 to 75 degrees centigrade to extract active compounds of Curcuma Caesia including Ar-turmerone, Germacrone, Epicurzerenone, Curzerenone, Eucalyptol, and Camphor in the range of 85-97% of the steam distillation extraction solution; e) mixing Stigmasterol in the range of 0.1 % to 1.5% and P-sitosterol in the range of 0.1% to 1.5% with the steam distillation extraction solution in dark at room temperature to form a first solution; f) a second solution is prepared by adding 0.1% to 0.5% Vitamin A and 0.1% to 0.5% Quercetin in 99% virgin coconut oil; g) infusing 1 to 20 parts of the first solution with 80 to 99 parts of the second solution at a temperature 30-60°C with constant stirring at 600- 800 rpm for 2-6 hours then, allowed to cool down in dark at room temperature to make a third solution; h) mixing the third solution with desired amount of anti-caking agents, thickening agents, natural stabilizer, natural antifoaming agent,preservative, pH maintaining agent and anti-rancidity agent to form a fourth solution; and i) centrifuging the fourth solution at 4000-5000 rpm and then filtering to remove impurities to form the hair oil composition.

7. The method as claimed in claim 6, wherein the anti-caking agents and thickening agents maintain the viscosity of the hair oil composition in the range of 30-38 cP.

8. The method as claimed in claim 6, wherein the anti-rancidity agent in the range of 0.01%-0.5% is added to avoid photocatalytic oxidation of the hair oil composition.

9. The method as claimed in claim 6, wherein the Stigmasterol and the P-sitosterol are allowed to completely soluble with the steam distillation extraction solution using sonication at 35 to 45 degrees centigrade for 1-3 hours.

10. The method as claimed in claim 6, wherein the pH of the hair oil composition is maintained between 4.5 and 5.

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