Method for preparing composite pig manure fermentation inoculant and fermentation inoculant prepared thereby, and pig manure bio-organic fertilizer, preparation method therefor and use thereof

Abstract

The present invention relates to the technical field of microorganism application. Disclosed are a method for preparing a composite pig manure fermentation inoculant and a fermentation inoculant prepared thereby, and a pig manure bio-organic fertilizer, a preparation method therefor and the use thereof. The method for preparing the inoculant comprises: S1, respectively performing fermentation using five strains of inoculants, namely Bacillus.spHZ3, Geobacillus stearothermophilus, Alcaligenes faecalis subsp.Faecalis, Streptomyces levoris and Aspergillus oryzae, respectively, to obtain fresh fermentation broths of the five strains; S2, mixing the fresh fermentation broths of the five strains according to a volume ratio of 3-5 : 1-3 : 1-2 : 2-3 : 1-3 to obtain a composite inoculant; and S3, further adding a flora accelerant to obtain the inoculant. The method for preparing the bio-organic fertilizer comprises: (1) adding an oxidizing agent and an activating agent to pig manure for pretreatment, (2) adding the inoculant to ferment the pig manure, and (3) drying and crushing same to obtain the bio-organic fertilizer. By coupling oxidation treatment technology with microbial fermentation treatment, the composting time is shortened, the conversion of organic matters is improved, the manurial effect of the organic fertilizer is enhanced, and the effect of rapidly preparing an organic fertilizer is achieved.

Description

Preparation method of pig manure composite fermentation agent, fermentation agent prepared therefrom, pig manure bio-organic fertilizer, and preparation method and application thereof Technical Field

[0001] The present invention relates to the technical field of microbial application, and in particular to a method for preparing a pig manure composite fermentation agent, a fermentation agent prepared using the same, a pig manure bio-organic fertilizer, and a preparation method and application thereof. Background Art

[0002] As people's living standards continue to improve, the demand for livestock and poultry products is also increasing. The Food and Agriculture Organization of the United Nations (FAO) predicts that global demand for meat products will increase by 73% by 2050. This rapid growth in demand for livestock and poultry products has also led to a shift in the livestock industry from traditional free-range farming to intensive, large-scale operations, with the scale of farming expanding. The emergence of large-scale pig farms with a single output of over one million pigs has dramatically increased the pressure on livestock and poultry manure disposal. Statistics show that during the 13th Five-Year Plan period, my country generated approximately 3.8 billion tons of livestock and poultry manure annually, with a comprehensive utilization rate of less than 60%. Pig manure produced the largest amount of this waste annually, at approximately 1.8 billion tons, accounting for 47% of the total. The accumulation of large amounts of livestock and poultry manure not only occupies land and wastes resources, but also produces harmful gases such as hydrogen sulfide and ammonia, polluting the environment and seriously endangering human health. Therefore, strengthening the harmless and resource-based treatment of livestock and poultry manure is urgently needed to protect the ecological environment and develop the livestock industry.

[0003] Livestock and poultry manure contains a variety of nutrients such as protein, fat, carbohydrates, and a large number of other beneficial substances. Converting livestock and poultry manure into organic fertilizer not only provides comprehensive nutrition for crops, but also increases and renews soil organic matter, promotes microbial reproduction, and improves the physical and chemical properties and biological activity of the soil. However, traditional organic fertilizer production generally takes 45-60 days to complete. The 45-60 day fermentation cycle is too long for some fertilizer companies / manure treatment centers / farms. Not only does it require a large amount of equipment and space for production, but manure that is not processed in time will further produce malodorous gases such as methyl sulfide, dimethyl disulfide, methyl sulfide, dimethylamine, and low-level fatty acids during the stacking process, which will have adverse effects on the health of people on site and in the surrounding area and cause new environmental pollution. Therefore, timely processing of livestock and poultry manure and rapid preparation of organic fertilizer is one of the important issues that need to be urgently addressed in the development of green and ecological farming in my country.

[0004] At present, traditional organic fertilizer production mostly adopts natural composting fermentation or composting with fermentation agents. It needs to go through a heating period, a high temperature period, a cooling period, a decomposition and fertilizer preservation stage, etc. Under normal circumstances, it takes 45-60 days to ferment high-quality organic fertilizer. Although the production cycle can be shortened to 7-10 days using organic fertilizer fermentation tanks, the processing capacity is relatively small, and special fermentation equipment needs to be purchased separately. The production cost is still very high, and large-scale promotion is limited. Therefore, the development of pig manure organic fertilizer preparation technology that can quickly decompose pig manure while retaining the advantages of rich and balanced nutrition of organic fertilizer and easy to promote on a large scale is of great significance for solving the environmental pollution problem caused by pig manure and promoting the healthy development of the pig industry.

[0005] The Chinese patent application document with publication number CN105255777A discloses a microorganism Bacillus subtilis Lys-979 used for efficient and rapid fermentation of pig manure and mushroom residue and a process for fermenting and preparing bio-organic fertilizer; the deposit number of Bacillus subtilis Lys-979 is CGMCC No.11333. The fermentation of pig manure and mushroom residue using the strain completes the rapid decomposition of the fermentation base material in 18 to 22 days, and the physical and chemical indicators meet the new standards for organic fertilizers; and on this basis, it is compounded with a functional biocontrol agent to prepare a bio-organic fertilizer with a control function for crop diseases and insect pests, fundamentally solving the current problem of pollution and high cost of producing agricultural and animal husbandry feces and straw waste residue, and promoting the sustainable development of agricultural production. However, the time for preparing organic fertilizer is still too long, up to more than 20 days, and the quality of organic fertilizer also needs to be further improved.

[0006] Summary of the Invention

[0007] The technical problem to be solved by the present invention is how to solve the problems that the production of traditional pig manure organic fertilizer takes a long time to compost, is prone to secondary pollution and has low fertilizer efficiency.

[0008] Strain Description: The strains used in the microbial fermentation process of the present invention include:

[0009] Bacillus spHZ3, deposited with the China Center for Type Culture Collection (CCTCC). Deposit address: Luojia Mountain, Bayi Road, Wuchang District, Wuhan City, Hubei Province. Deposit number: CCTCC M 20221472, deposit date: September 21, 2022. This strain exhibits excellent antagonistic properties against common fungal pathogens, such as Fusarium graminearum and Phytophthora capsici.

[0010] Geobacillus stearothermophilus, accession number: CGMCC 1.1865; Alcaligenes faecalis subsp. Faecalis, accession number: CGMCC 1.3420; Streptomyces levoris, accession number: CGMCC 4.71; and Aspergillus oryzae, accession number: CGMCC 3.13905, were all purchased from the China General Culture Collection (CGMCC). They were screened using selective media, including cellulolytic bacteria selection medium, milk agar medium, and rhodamine B medium. Strains with good compatibility and strong ability to decompose organic matter were selected for scientific combination. The optimal addition ratio of each strain and the optimal amount of the stimulant were also studied.

[0011] The present invention solves the above technical problems through the following technical means:

[0012] A method for preparing a pig manure composite fermentation agent comprises the following steps:

[0013] S1: Single-strain fermentation: Ferment five bacterial strains, namely Bacillus spHZ3, Geobacillus stearothermophilus, Alcaligenes, Streptomyces, and Aspergillus oryzae, to obtain fresh fermentation broths of the five bacterial strains;

[0014] S2: preparing a composite bacterial agent: mixing the fresh fermentation broth of five strains of Bacillus spHZ3, Geobacillus stearothermophilus, Alcaligenes sp., Streptomyces sp., and Aspergillus oryzae obtained in step S1 in a volume ratio of (3-5):(1-3):(1-2):(2-3):(1-3) to obtain a composite bacterial agent;

[0015] S3: Adding a bacterial colony promoter: Adding a bacterial colony promoter to the composite bacterial agent obtained in step S2 to prepare a pig manure composite fermentation bacterial agent.

[0016] Beneficial effect: The present invention selects five special strains, mixes them in a special ratio, and then adds a flora promoter to prepare a composite fermentation agent that can efficiently ferment pig manure, thereby shortening the preparation time of pig manure organic fertilizer and improving the fertilizer efficiency of pig manure organic fertilizer.

[0017] Preferably, the specific operation steps of fermenting the five bacterial strains separately in step S1 are:

[0018] Bacillus sppHZ3, Geobacillus stearothermophilus, and Alcaligenes were cultured in LBG medium containing 0.5% glucose, 1% peptone, 0.5% yeast extract, 1% sodium chloride, and 1000 mL of pure water. The culture was inoculated at a 1% (v / v) inoculum into the LBG liquid medium at 150-200 rpm for 12-24 hours.

[0019] Streptomyces was cultured using SCP medium consisting of 1.0% sucrose, 2.0% cornstarch, 0.3% peptone, 3.0% soybean meal, 0.1% potassium dihydrogen phosphate, 0.5% ammonium sulfate, 0.1% sodium chloride, 0.3% calcium carbonate, and 1000 mL of pure water. A 1% (v / v) inoculum was inoculated into the SCP liquid medium at 150-200 rpm for 12-24 hours.

[0020] Aspergillus oryzae uses MSP culture medium, which consists of 3% corn cob powder, 0.2% peptone, 0.4% KH2PO4, 0.04% (w / v) CaCl2, 0.04% MgSO4, and 1000mL pure water; the culture medium is inoculated at a 2% (v / v) inoculum volume into the MSP liquid culture medium, at 150-200rpm, and cultured for 12-24h.

[0021] Preferably, in step S3, a bacterial colony promoter is added to the composite bacterial agent obtained in step S2 at a ratio of 1-2% (w / v).

[0022] Preferably, the flora promoter in step S3 is prepared by mixing tea polyphenols and biotin in a mass ratio of (3-5): (1-3).

[0023] The present invention also provides a pig manure composite fermentation agent prepared by the method for preparing the pig manure composite fermentation agent.

[0024] The present invention also provides a method for rapidly composting and preparing pig manure bio-organic fertilizer, comprising the following steps:

[0025] (1) Oxidation treatment of pig manure: add oxidant and activator to fresh pig manure, mix thoroughly, and stir at room temperature for a period of time to obtain the initial conversion product of pig manure;

[0026] Note: After the pig manure is oxidized, the initial transformation products obtained have a high degree of organic matter stabilization and humification.

[0027] (2) Microbial fermentation of the primary conversion product of pig manure: spray the pig manure composite fermentation agent prepared by the above method onto the primary conversion product of pig manure obtained in step (1), fully mix and evenly mix, adjust the water content of the primary conversion product of pig manure, ferment at room temperature, turn the pile once every period of time, ferment for several days, and stop fermenting when the primary conversion product of pig manure turns dark brown and the temperature drops to room temperature to obtain a completely fermented product of pig manure;

[0028] (3) Preparation of pig manure bio-organic fertilizer: The pig manure complete fermentation product obtained in step (2) is dried and crushed to obtain pig manure bio-organic fertilizer.

[0029] Beneficial effects: The present invention couples oxidation treatment technology with microbial fermentation treatment, which significantly shortens the time required for composting, improves the conversion of organic matter, enhances the fertilizer efficiency of organic fertilizer, and achieves the effect of quickly preparing high-quality bio-fertilizer.

[0030] Preferably, the oxidant in step (1) is a mixture of two or more of sodium persulfate, ferrous sulfate, calcium hydroxide, and magnesium oxide.

[0031] Preferably, the oxidant in step (1) is a mixture of sodium persulfate and ferrous sulfate in a mass ratio of 1:1.

[0032] Preferably, the oxidant in step (1) is a mixture of sodium persulfate, ferrous sulfate and calcium hydroxide in a mass ratio of 5:2:1.

[0033] Preferably, the oxidant in step (1) is a mixture of sodium persulfate, calcium hydroxide and magnesium oxide in a mass ratio of 4:1:2.

[0034] Preferably, the activator in step (1) is prepared by the following process: dissolving FeSO4·7H2O in deoxygenated water, adding biological activated carbon, adjusting the pH of the solution, shaking, and then continuously introducing N2 while stirring while dropwise adding KBH4 solution, washing, and drying to obtain the activator; the mass ratio of the FeSO4·7H2O to the volume of deoxygenated water is 3g / 100mL; the mass ratio of the biological activated carbon to FeSO4·7H2O is (3-5):15.

[0035] Preferably, the concentration of the KBH4 solution is 0.2 mol / L.

[0036] Preferably, the activator in step (1) is prepared by the following process: 1.5 g FeSO4·7H2O is dissolved in 50 mL of deoxygenated water, 0.3 g to 0.5 g of biological activated carbon is added, the pH of the system is adjusted to 4 to 6 with 0.5 mol / L NaOH and HCl, and the mixture is shaken in a shaker for 10 to 18 h to fully adsorb Fe 2+Subsequently, 0.2 mol / L KBH4 solution was added dropwise (drop rate 3-5 mL / min), while N2 was continuously introduced and stirred for 6-8 h. The biological activated carbon was quickly washed 2-3 times with anhydrous ethanol and deoxygenated water, and dried to obtain an activator loaded with zero-valent iron.

[0037] Preferably, the stirring time in step (1) is 30-60 min.

[0038] Preferably, the dosage of the pig manure composite fermentation agent in step (2) is: spraying 5-10 mL of the pig manure composite fermentation agent on every 1000 g of the pig manure preliminary conversion product.

[0039] Preferably, in step (2), the water content of the primary pig manure conversion product is adjusted to 50-60%.

[0040] Preferably, in step (2), the pile is turned once every 8-16 hours.

[0041] The present invention also provides a pig manure bio-organic fertilizer prepared by adopting the rapid decomposition preparation method of the pig manure bio-organic fertilizer.

[0042] The present invention also provides an application of the pig manure bio-organic fertilizer prepared by adopting the rapid decomposition preparation method of the pig manure bio-organic fertilizer in agricultural production.

[0043] The advantages of the present invention are:

[0044] (1) The present invention selects five special strains, mixes them in a special ratio, and then adds a flora promoter to prepare a composite fermentation agent that can efficiently ferment pig manure, thereby shortening the preparation time of pig manure organic fertilizer and improving the fertilizer efficiency of pig manure organic fertilizer.

[0045] (2) The present invention couples oxidation treatment technology with microbial fermentation treatment to rapidly destroy the solid structure of organic nutrients in pig manure through oxidation treatment, producing substances such as small molecular carbon sources and nitrogen sources, which are then utilized by microorganisms in the biological treatment section. The present invention not only efficiently and quickly achieves rapid humification of pig manure, but also fully utilizes potential carbon sources, realizes the reproduction of beneficial microorganisms (especially microorganisms with biocontrol functions), and produces high-quality bio-organic fertilizer. BRIEF DESCRIPTION OF THE DRAWINGS

[0046] FIG1 is a graph showing the inhibitory effect of strain Bacillus.sp HZ3 on the growth of pathogenic fungi such as Fusarium graminearum and Phytophthora capsici;

[0047] FIG2 is a comparison of three-dimensional fluorescence spectra of pig manure before (A) and after (B) oxidant treatment;

[0048] FIG3 is a three-dimensional fluorescence spectrum analysis diagram of the bio-organic fertilizer prepared after oxidant treatment + microbial fermentation;

[0049] FIG4 is a graph showing the effect of oxidant treatment coupled with composite bacterial fermentation on the total humic acid content of pig manure compost;

[0050] Figure 5 shows the effect of adding activator B on the water-soluble humic acid content in pig manure after oxidant treatment. DETAILED DESCRIPTION

[0051] To make the objectives, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts shall fall within the scope of protection of the present invention.

[0052] The sodium persulfate, ferrous sulfate, calcium hydroxide and magnesium oxide used in the present invention are all purchased from the market and produced by Sinopharm Chemical Reagent Co., Ltd.; the biological activated carbon is also purchased from the market and produced by Pingdingshan Green Origin Activated Carbon Co., Ltd.

[0053] Example 1:

[0054] A method for preparing a pig manure composite fermentation agent comprises the following steps:

[0055] S1: Single-strain fermentation: Bacillus spHZ3, Geobacillus stearothermophilus, Alcaligenes sp., Streptomyces sp., and Aspergillus oryzae sp. were fermented separately to obtain fresh fermentation broths of the five strains. Bacillus spHZ3, Geobacillus stearothermophilus, and Alcaligenes sp. were cultured in LBG medium (0.5% glucose, 1% peptone, 0.5% yeast extract, 1% sodium chloride, and 1000 mL of purified water). The culture was inoculated at a 1% (v / v) inoculum into the LBG liquid medium at 150 rpm for 12 h.

[0056] Streptomyces was cultured using SCP medium consisting of 1.0% sucrose, 2.0% cornstarch, 0.3% peptone, 3.0% soybean meal, 0.1% potassium dihydrogen phosphate, 0.5% ammonium sulfate, 0.1% sodium chloride, 0.3% calcium carbonate, and 1000 mL of pure water. The culture was inoculated at a 1% (v / v) inoculum into the SCP liquid medium at 150 rpm for 12 h.

[0057] Aspergillus oryzae was cultured in MSP medium containing 3% corncob powder, 0.2% peptone, 0.4% KH2PO4, 0.04% (w / v) CaCl2, 0.04% MgSO4, and 1000 mL of pure water. The culture medium was inoculated at a 2% (v / v) inoculum volume into the MSP liquid medium at 150 rpm for 12 h.

[0058] S2: preparing a composite bacterial agent: mixing the fresh fermentation broth of five strains of Bacillus spHZ3, Geobacillus stearothermophilus, Alcaligenes, Streptomyces, and Aspergillus oryzae obtained in step S1 in a volume ratio of 3:1:1:2:1 to obtain a composite bacterial agent;

[0059] S3: Adding a flora promoter: Add a flora promoter to the composite bacterial agent obtained in step S2 at a ratio of 1% (w / v) to prepare a pig manure composite fermentation bacterial agent; the flora promoter is prepared by mixing tea polyphenols and biotin at a mass ratio of 3:1.

[0060] The pig manure composite fermentation agent of this embodiment is named pig manure composite fermentation agent a.

[0061] Example 2:

[0062] A method for preparing a pig manure composite fermentation agent comprises the following steps:

[0063] S1: Single-strain fermentation: Bacillus spHZ3, Geobacillus stearothermophilus, Alcaligenes sp., Streptomyces sp., and Aspergillus oryzae sp. were fermented separately to obtain fresh fermentation broths of the five strains. Bacillus spHZ3, Geobacillus stearothermophilus, and Alcaligenes sp. were cultured in LBG medium (0.5% glucose, 1% peptone, 0.5% yeast extract, 1% sodium chloride, and 1000 mL of purified water). The culture was inoculated at a 1% (v / v) inoculum into the LBG liquid medium at 180 rpm for 20 h.

[0064] Streptomyces was cultured using SCP medium consisting of 1.0% sucrose, 2.0% cornstarch, 0.3% peptone, 3.0% soybean meal, 0.1% potassium dihydrogen phosphate, 0.5% ammonium sulfate, 0.1% sodium chloride, 0.3% calcium carbonate, and 1000 mL of pure water. The culture was inoculated at a 1% (v / v) inoculum into the SCP liquid medium at 180 rpm for 20 h.

[0065] Aspergillus oryzae was cultured in MSP medium containing 3% corncob powder, 0.2% peptone, 0.4% KH2PO4, 0.04% (w / v) CaCl2, 0.04% MgSO4, and 1000 mL of pure water. The culture medium was inoculated at a 2% (v / v) inoculum volume into the MSP liquid medium at 180 rpm for 20 h.

[0066] S2: preparing a composite bacterial agent: mixing the fresh fermentation broth of five strains of Bacillus spHZ3, Geobacillus stearothermophilus, Alcaligenes, Streptomyces, and Aspergillus oryzae obtained in step S1 in a volume ratio of 3:2:1:2:3 to obtain a composite bacterial agent;

[0067] S3: Adding a flora promoter: Add a flora promoter to the composite bacterial agent obtained in step S2 at a ratio of 1.5% (w / v) to prepare a pig manure composite fermentation bacterial agent; the flora promoter is prepared by mixing tea polyphenols and biotin at a mass ratio of 5:2.

[0068] The pig manure composite fermentation agent prepared in this example is named pig manure composite fermentation agent b.

[0069] Example 3:

[0070] A method for preparing a pig manure composite fermentation agent comprises the following steps:

[0071] S1: Single-strain fermentation: Bacillus spHZ3, Geobacillus stearothermophilus, Alcaligenes sp., Streptomyces sp., and Aspergillus oryzae sp. were fermented separately to obtain fresh fermentation broths of the five strains. Bacillus spHZ3, Geobacillus stearothermophilus, and Alcaligenes sp. were cultured in LBG medium (0.5% glucose, 1% peptone, 0.5% yeast extract, 1% sodium chloride, and 1000 mL of purified water). The culture was inoculated at a 1% (v / v) inoculum size into the LBG liquid medium at 200 rpm for 24 h.

[0072] Streptomyces was cultured using SCP medium consisting of 1.0% sucrose, 2.0% cornstarch, 0.3% peptone, 3.0% soybean meal, 0.1% potassium dihydrogen phosphate, 0.5% ammonium sulfate, 0.1% sodium chloride, 0.3% calcium carbonate, and 1000 mL of pure water. A 1% (v / v) inoculum was inoculated into the SCP liquid medium at 200 rpm for 24 h.

[0073] Aspergillus oryzae was cultured in MSP medium containing 3% corncob powder, 0.2% peptone, 0.4% KH2PO4, 0.04% (w / v) CaCl2, 0.04% MgSO4, and 1000 mL of pure water. The culture medium was inoculated at a 2% (v / v) inoculum volume into the MSP liquid medium at 200 rpm for 24 h.

[0074] S2: preparing a composite bacterial agent: mixing the fresh fermentation broth of five strains of Bacillus spHZ3, Geobacillus stearothermophilus, Alcaligenes, Streptomyces, and Aspergillus oryzae obtained in step S1 in a volume ratio of 5:3:2:3:2 to obtain a composite bacterial agent;

[0075] S3: Adding a flora promoter: Add a flora promoter to the composite bacterial agent obtained in step S2 at a ratio of 2% (w / v) to prepare a pig manure composite fermentation bacterial agent; the flora promoter is prepared by mixing tea polyphenols and biotin at a mass ratio of 4:3.

[0076] The pig manure composite fermentation agent prepared in this example is named pig manure composite fermentation agent c.

[0077] Example 4:

[0078] A method for rapidly composting and preparing pig manure bio-organic fertilizer comprises the following steps:

[0079] (1) Oxidation treatment of pig manure: Add 0.2g of oxidant and 0.1g of activator to fresh pig manure, mix thoroughly, and stir at room temperature for 30min to obtain a preliminary conversion product of pig manure with a high degree of organic matter stabilization and humification;

[0080] The oxidant was prepared by mixing sodium persulfate and ferrous sulfate in a mass ratio of 1:1. The activator was prepared by the following process: 1.5 g FeSO4·7H2O was dissolved in 50 mL of deoxygenated water, 0.3 g of biological activated carbon was added, the pH of the system was adjusted to 4 with 0.5 mol / L NaOH and HCl, and the mixture was shaken in a shaker for 10 h to fully adsorb Fe 2+ Subsequently, 0.2 mol / L KBH4 solution was added dropwise (drop rate 3 mL / min), while N2 was continuously introduced and stirred for 6 h. The biological activated carbon was quickly washed twice with anhydrous ethanol and deoxygenated water, and dried to obtain the activator loaded with zero-valent iron.

[0081] (2) Microbial fermentation of the primary conversion product of pig manure: 5 mL of the pig manure composite fermentation agent a prepared in Example 1 was sprayed onto 1000 g of the primary conversion product of pig manure obtained in step (1), and the mixture was thoroughly mixed. The water content of the primary conversion product of pig manure was adjusted to 50%, and the mixture was fermented at room temperature. The mixture was turned over once every 12 hours. After 5 days of fermentation, the primary conversion product of pig manure was dark brown, and the temperature dropped to room temperature, indicating that the fermentation was complete. The fermentation was stopped to obtain a completely fermented product of pig manure.

[0082] (3) Preparation of pig manure bio-organic fertilizer: The pig manure complete fermentation product obtained in step (2) is dried and crushed to obtain pig manure bio-organic fertilizer rich in beneficial bacteria.

[0083] The pig manure bio-organic fertilizer prepared in this embodiment was tested, and the experimental results of the test were:

[0084] The effective viable bacteria count of the prepared bio-organic fertilizer was determined according to the method in NY / T 2321-2013 “Inspection Procedure for Microbial Fertilizer Products”, and the effective viable bacteria count was 3.8×10 8 CFU·g -1 The organic matter content and pH value of the bio-organic fertilizer product were determined according to the method in NY884-2012 "Bio-organic Fertilizer". The organic matter content was 46.8% and the pH value was 7.2, which met the technical requirements of the bio-organic fertilizer product.

[0085] Example 5:

[0086] A method for rapidly composting and preparing pig manure bio-organic fertilizer comprises the following steps:

[0087] (1) Oxidation treatment of pig manure: Add 0.4 g of oxidant and 0.2 g of activator to fresh pig manure, mix thoroughly, and stir at room temperature for 50 min to obtain a preliminary conversion product of pig manure with a high degree of organic matter stabilization and humification;

[0088] The oxidant was prepared by mixing sodium persulfate, ferrous sulfate, and calcium hydroxide in a mass ratio of 5:2:1. The activator was prepared by dissolving 1.5 g of FeSO4·7H2O in 50 mL of deoxygenated water, adding 0.4 g of biological activated carbon, adjusting the pH of the system to 4.5 with 0.5 mol / L NaOH and HCl, and shaking on a shaker for 15 h to fully adsorb Fe. 2+ Subsequently, 0.2 mol / L KBH4 solution was added dropwise (drop rate 4 mL / min), while N2 was continuously introduced and stirred for 8 h. The biological activated carbon was quickly washed with anhydrous ethanol and deoxygenated water three times and dried to obtain the activator loaded with zero-valent iron.

[0089] (2) Microbial fermentation of the primary conversion product of pig manure: 8 mL of the pig manure composite fermentation agent b prepared in Example 2 was sprayed onto 1000 g of the primary conversion product of pig manure obtained in step (1), and the mixture was thoroughly mixed. The water content of the primary conversion product of pig manure was adjusted to 55%, and the mixture was fermented at room temperature. The mixture was turned over every 8 h. After 3 days of fermentation, the primary conversion product of pig manure was dark brown, and the temperature dropped to room temperature, indicating that the fermentation was complete. The fermentation was stopped to obtain a completely fermented product of pig manure.

[0090] (3) Preparation of pig manure bio-organic fertilizer: The pig manure complete fermentation product obtained in step (2) is dried and crushed to obtain pig manure bio-organic fertilizer rich in beneficial bacteria.

[0091] The pig manure bio-organic fertilizer prepared in this embodiment was tested, and the experimental results of the test were:

[0092] The effective viable bacteria count of the prepared bio-organic fertilizer was determined according to the method in NY / T 2321-2013 “Inspection Procedure for Microbial Fertilizer Products” and the effective viable bacteria count was 5.3×10 8 CFU·g -1 The organic matter content and pH value of the bio-organic fertilizer product were determined according to the method in NY884-2012 "Bio-organic Fertilizer". The organic matter content was 56.7% and the pH value was 7.5, which met the technical requirements of the bio-organic fertilizer product.

[0093] Example 6:

[0094] A method for rapidly composting and preparing pig manure bio-organic fertilizer comprises the following steps:

[0095] (1) Oxidation treatment of pig manure: Add 0.5g of oxidant and 0.3g of activator to fresh pig manure, mix thoroughly, and stir at room temperature for 60min to obtain a preliminary conversion product of pig manure with a high degree of organic matter stabilization and humification;

[0096] The oxidant was prepared by mixing sodium persulfate, calcium hydroxide, and magnesium oxide in a mass ratio of 4:1:2. The activator was prepared by dissolving 1.5 g of FeSO4·7H2O in 50 mL of deoxygenated water, adding 0.5 g of biological activated carbon, adjusting the pH of the system to 6 with 0.5 mol / L NaOH and HCl, and shaking on a shaker for 18 h to fully adsorb Fe. 2+ Subsequently, 0.2 mol / L KBH4 solution was added dropwise (drop rate 5 mL / min), while N2 was continuously introduced and stirred for 7 h. The biological activated carbon was quickly washed with anhydrous ethanol and deoxygenated water three times and dried to obtain the activator loaded with zero-valent iron.

[0097] (2) Microbial fermentation of the primary conversion product of pig manure: 10 mL of the pig manure composite fermentation agent c prepared in Example 3 was sprayed onto 1000 g of the primary conversion product of pig manure obtained in step (1), and the mixture was thoroughly mixed. The water content of the primary conversion product of pig manure was adjusted to 60%, and the mixture was fermented at room temperature. The mixture was turned over once every 16 h. After 7 days of fermentation, the primary conversion product of pig manure was dark brown, and the temperature dropped to room temperature, indicating that the fermentation was complete. The fermentation was stopped to obtain a completely fermented product of pig manure.

[0098] (3) Preparation of pig manure bio-organic fertilizer: The pig manure complete fermentation product obtained in step (2) is dried and crushed to obtain pig manure bio-organic fertilizer rich in beneficial bacteria.

[0099] The pig manure bio-organic fertilizer prepared in this embodiment was tested, and the experimental results of the test were:

[0100] The effective viable bacteria count of the prepared bio-organic fertilizer was determined according to the method in NY / T 2321-2013 “Inspection Procedure for Microbial Fertilizer Products”, and the effective viable bacteria count was 4.1×10 8 CFU·g -1 The organic matter content and pH value of the bio-organic fertilizer product were determined according to the method in NY884-2012 "Bio-organic Fertilizer". The organic matter content was 50.3% and the pH value was 7.3, which met the technical requirements of the bio-organic fertilizer product.

[0101] Example 7:

[0102] Implementation case of applying the bio-organic fertilizer prepared in the present invention in corn production

[0103] Test crop: corn "Fengle 358"

[0104] Fertilizers used: Bio-organic fertilizers prepared in Examples 4-6, and organic fertilizers prepared by traditional composting methods, all applied at a rate of 3000 kg / hm2. 2 All fertilizers were applied as base fertilizer to the topsoil at once, and no topdressing was required during the entire corn growing period. Other management measures were consistent with those in local areas. A blank control was established, meaning no fertilizer was applied.

[0105] Measurement Indicators: During the corn harvest period, ears of 30 consecutive corn plants of consistent growth were collected from each plot to measure aboveground biomass, corn yield, and yield components. Additionally, surface soil samples (0-20 cm) were collected after corn harvest to measure soil nutrient content and enzyme activity.

[0106] The experimental results are shown in Table 1 below:

[0107] From the data in Table 1 we can see that the bio-organic fertilizer test group obtained by the embodiment of the present invention 4-6, the corn yield is much higher than the organic fertilizer and blank test group of traditional compost, and the regulation of the biological activity of key soil enzymes is also very significant. Changing the ratio and composition of the oxidant and the activator, or the ratio of the composite microbial agent, whether it is fertilizer efficiency or the regulating effect on soil, has all presented obvious changes. Therefore, the composition and preparation technology of the composite microbial agent in the present invention are the key to realizing the stable fertilizer efficiency of the present invention and the soil conditioning effect, and lacking one will result in a weak effect.

[0108] Figure 1 shows the inhibitory effect of strain Bacillus.sp HZ3 on the growth of pathogenic fungi such as Fusarium graminearum and Phytophthora capsici. As can be seen from the figure, an obvious inhibition zone appears around the Bacillus.sp HZ3 strain, indicating that the biocontrol active substances produced by it inhibit the growth of pathogenic fungi such as Fusarium graminearum and Phytophthora capsici.

[0109] Figure 2 shows a comparison of three-dimensional fluorescence spectra of pig manure before (A) and after (B) oxidant treatment. The figure shows that after oxidant treatment, characteristic peaks with excitation / emission wavelengths of 330–360 / 400–460 nm appear, indicating the generation of humic acid substances (indicated by the black arrows).

[0110] Figure 3 is a three-dimensional fluorescence spectrum analysis diagram of the bio-organic fertilizer prepared after oxidant treatment + microbial fermentation; it can be seen from the figure that the bio-organic fertilizer prepared after oxidant treatment + microbial fermentation contains very rich humic acid (indicated by the black arrow).

[0111] Figure 4 shows the effect of oxidant treatment coupled with composite bacterial fermentation on the total humic acid content of pig manure compost; it can be seen from the figure that the total humic acid carbon content in the biological organic fertilizer prepared by oxidant treatment + inoculation with composite bacterial agent is much higher than that in the biological organic fertilizer prepared by composting without inoculation with bacterial agent or composting with only inoculation with composite bacterial agent.

[0112] Figure 5 shows the effect of adding activator B on the water-soluble humic acid content in pig manure after oxidant treatment. It can be seen from the figure that the carbon content of water-soluble humic acid in the bio-organic fertilizer prepared after adding the activator is much higher than that of the bio-organic fertilizer prepared without adding the activator.

[0113] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims

1. A method for preparing a pig manure composite fermentation agent, characterized in that: The following steps are involved: S1: Single strain fermentation: Ferment five strains of bacteria, namely Bacillus spHZ3, Bacillus stearothermophilus, Alcaligenes, Streptomyces and Aspergillus oryzae, respectively, to obtain fresh fermentation broth of the five strains; S2: preparing a composite bacterial agent: mixing the fresh fermentation broth of five strains of Bacillus spHZ3, Bacillus stearothermophilus, Alcaligenes, Streptomyces and Aspergillus oryzae obtained in step S1 in a volume ratio of 3-5:1-3:1-2:2-3:1-3 to obtain a composite bacterial agent; S3: Adding a bacterial flora promoter: Adding a bacterial flora promoter to the composite bacterial agent obtained in step S2, thereby obtaining a pig manure composite fermentation bacterial agent.

2. The method for preparing the pig manure composite fermentation agent according to claim 1, characterized in that: In the step S3, a flora promoter is added to the composite bacterial agent obtained in the step S2 at a ratio of 1-2% (w / v); the flora promoter is prepared by mixing tea polyphenols and biotin at a mass ratio of 3-5:1-3.

3. A pig manure composite fermentation agent prepared by the preparation method according to any one of claims 1 to 2.

4. A method for preparing a rapid decomposition of pig manure bio-organic fertilizer, characterized in that: The following steps are involved: (1) Oxidation treatment of pig manure: add oxidant and activator to fresh pig manure, mix thoroughly and evenly, and stir at room temperature for a period of time to obtain the initial conversion product of pig manure; (2) Microbial fermentation of the preliminary conversion product of pig manure: spraying the composite fermentation agent of pig manure obtained by the preparation method according to any one of claims 1 to 2 onto the preliminary conversion product of pig manure obtained in step (1), mixing thoroughly and evenly, adjusting the water content of the preliminary conversion product of pig manure, fermenting at room temperature, turning the pile once every certain period of time, and after fermenting for several days, when the preliminary conversion product of pig manure turns dark brown and the temperature drops to room temperature, stopping the fermentation to obtain a complete fermentation product of pig manure; (3) Preparation of pig manure bio-organic fertilizer: The pig manure complete fermentation product obtained in step (2) is dried and crushed to obtain pig manure bio-organic fertilizer.

5. The method for preparing the rapid decomposition of pig manure bio-organic fertilizer according to claim 4, characterized in that: The oxidant in step (1) is a mixture of two or more of sodium persulfate, ferrous sulfate, calcium hydroxide and magnesium oxide.

6. The method for preparing the rapid decomposition of pig manure bio-organic fertilizer according to claim 4, characterized in that: The activator in step (1) is prepared by the following process: dissolving FeSO4·7H2O in deoxygenated water, adding biological activated carbon, adjusting the pH value of the solution, oscillating, then dropping KBH4 solution while continuously introducing N2 and stirring, washing, and drying to obtain the activator; the mass ratio of the FeSO4·7H2O to the volume of deoxygenated water is 3g / 100mL; the mass ratio of the biological activated carbon to FeSO4·7H2O is 3-5:

15.

7. The method for preparing the rapid decomposition of pig manure bio-organic fertilizer according to claim 4, characterized in that: The stirring time in step (1) is 30-60 min.

8. The method for preparing the rapid decomposition of pig manure bio-organic fertilizer according to claim 4, characterized in that: The dosage of the pig manure composite fermentation agent in the step (2) is as follows: spray 5-10 mL of the pig manure composite fermentation agent on every 1000 g of the pig manure preliminary conversion product; adjust the water content of the pig manure preliminary conversion product to 50-60%; and turn the pile once every 8-16 hours.

9. Pig manure bio-organic fertilizer prepared by the preparation method according to any one of claims 4 to 8.

10. Application of the pig manure bio-organic fertilizer prepared by the preparation method according to any one of claims 4 to 8 in agricultural production.

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