Peptide conjugates derived from natural toxin and machine learning, binding to the claudin-4 (CLDN4) for the radionuclide imaging and therapy of pancreatic cancer

A chimeric peptide targeting claudin-4 allows for precise and controlled gene editing in specific cells within the tomato fruit, addressing the challenges of temporal and spatial control in current gene expression methods.

WO2025076450A1PCT designated stage expired Publication Date: 2025-04-10THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIV
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Patent Information

Application Number
PCT/US2024/050092
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2023-10-05
Filing Date
2024-10-04
Publication Date
2025-04-10

AI Technical Summary

Technical Problem

Current methods for targeted gene expression in specific cell types, such as those using Cre recombinase, face challenges in achieving precise temporal and spatial control, particularly in complex tissues like the tomato fruit.

Method used

Development of a chimeric peptide comprising a chelator, spacer, and claudin-4 binding sequence, which specifically targets and binds to claudin-4, a tight junction protein, allowing for controlled delivery of Cre recombinase or other effector molecules into specific cells.

Benefits of technology

This approach enables precise and efficient targeted gene editing in specific cell types within the tomato fruit, overcoming the limitations of previous methods by providing temporal and spatial control over gene expression.

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Abstract

Chemical compounds have been designed specifically from Claudin-4 binder peptides (C4BP) for radiopharmaceuticals. Specifically, compounds and compositions integrating C4BP have been designed with the distinct application in pancreatic cancer detection and therapy. Embodiments are compounds having a peptide, spacer (or without spacer), and chelator where this compound binds to CLDN4. The compound can incorporate the metallic radioisotopes to produce radiopharmaceuticals. Applications for these compounds include diagnostic procedures, therapeutic interventions, and targeted delivery of radionuclides to tissues that express CLDN4. Methods detailed herein delineate the use of radiopharmaceutical with CPB for diagnosis, treatment, and targeted delivery within CLDN4 expressing tissues.
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Description

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Claims

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Citation Information

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