Directly determining signal-to-noise-ratio metrics for accelerated convergence in determining nucleotide-base calls and base-call quality

By directly determining signal-to-noise-ratio metrics, the method addresses noise-related challenges in nucleotide-base calling, improving sequencing accuracy and efficiency.

WO2025137341A1PCT designated stage expired Publication Date: 2025-06-26ILLUMINA INC
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Patent Information

Application Number
PCT/US2024/061114
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2024-04-22
Filing Date
2024-12-19
Publication Date
2025-06-26

AI Technical Summary

Technical Problem

Existing sequencing technologies face challenges in accurately determining nucleotide-base calls and assessing base-call quality due to high noise levels, which hinder efficient and reliable DNA sequencing.

Method used

A method is developed to directly determine signal-to-noise-ratio metrics for nucleotide-base calls, enhancing the accuracy and speed of base-call determination by optimizing sequencing algorithms and data processing techniques.

Benefits of technology

This approach improves the accuracy and efficiency of nucleotide-base calling by reducing noise-related errors, leading to enhanced sequencing performance and data quality.

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Abstract

This disclosure describes methods, non-transitory computer readable media, and systems that generate an improved signal-to-noise-ratio metric for light signals emitted from fluorescent tags of nucleotide bases during a sequence run and use such signal-to-noise-ratio metrics to determine more accurate and flexible base calls. For instance, the disclosed systems can detect a series of signals from labeled nucleotide bases of a nucleotide-sample slide, determine intensity correction parameters based on intensity values for a given sequencing cycle, determine a scaling factor and a noise level based on the intensity correction parameters, and generate a signal-to-noise-ratio metric for the given sequencing cycle based on the scaling factor and the noise level. The disclosed systems can further utilize the signal-to-noise-ratio to generate quality metrics for generated nucleobase calls.
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Citation Information

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  • Methods and systems for analyzing image data

    US10689696B2

  • Method of nucleic acid amplification

    US20050100900A1

  • Labelled nucleotides

    US20060188901A1

  • Modified polymerases for improved incorporation of nucleotide analogues

    US20060240439A1

  • Polymerases

    US20060281109A1