Fibroin peptides, protein fragments, and crosslinked fragments and compositions thereof

Abstract

The disclosure relates to peptides or protein fragments compositions, e.g., silk fibroin heavy chain, fibroin light chain, and / or fibrohexamerin (p25) derived peptides or protein fragments compositions.

Description

FIBROIN PEPTIDES, PROTEIN FRAGMENTS, AND CROSSLINKED FRAGMENTS AND COMPOSITIONS THEREOF CROSS REFERENCE TO RELATED APPLICATIONS This application claims priority to and the benefit of United States Provisional Patent Application No.63 / 567,877, filed on March 20, 2024, which is incorporated herein by reference in its entirety for any and all purposes. FIELD The disclosure relates to peptide compositions, e.g., silk fibroin derived peptide compositions. The present disclosure is also in the field of synthetic fabrics coated with silk fibroin proteins and protein fragments. In further embodiments, the present disclosure is in the field of silk fibroin compositions and methods for stimulating collagen and / or claudin-1 expression. BACKGROUND Silk is a natural polymer produced by a variety of insects and spiders, and comprises a filament core protein, silk fibroin, and a glue-like coating consisting of a non-filamentous protein, sericin. SUMMARY The disclosure provides peptide compositions, e.g., silk fibroin derived peptide compositions. The disclosure provides a peptide or protein fragment comprising a plurality of amino acids selected from M, R, V, K, T, F, I, L, C, A, Q, Y, N, D, E, G, S, H, P, and W, wherein at least one of the amino acids is modified, substituted, or replaced. In some embodiments, the peptide or protein fragment is a fibroin peptide or protein fragment comprising an amino acid modification, substitution, or replacement of an amino acid from of amino acids selected from M, R, V, K, T, F, I, L, C, A, Q, Y, N, D, E, G, S, H, P, and W. In some embodiments, the fibroin is a fibroin heavy chain, a fibroin light chain, or a fibrohexamerin. In some embodiments, the peptide or protein fragment comprises between about 2 and about 100 amino acids. In some embodiments, the peptide or protein fragment comprises between about 2 and about 25 amino acids. In some embodiments, the peptide or protein fragment comprises between about 25 and about 50 amino acids. In some embodiments, the peptide or protein fragment comprises between about 50 and about 75 amino acids. In some DB1 / 155183601.2 1embodiments, the peptide or protein fragment comprises between about 75 and about 100 amino acids. In some embodiments, the peptide or protein fragment comprises between about 100 and about 125 amino acids. In some embodiments, the peptide or protein fragment comprises between about 125 and about 150 amino acids. In some embodiments, the peptide or protein fragment comprises between about 150 and about 200 amino acids. In some embodiments, the peptide or protein fragment comprises between about 200 and about 250, 300, 350, 400, 450, or 500 amino acids. In some embodiments, the peptide or protein fragment comprises between one and five modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises one modification, substitution, and / or replacement. In some embodiments, the peptide or protein fragment comprises two modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises three modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises four modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises five modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises six modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises seven modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises eight modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises nine modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises ten modifications, substitutions, and / or replacements. In some embodiments, a modification, substitution, and / or replacement is selected from an asparagine to aspartic acid modification, substitution, and / or replacement, a glutamine to glutamic acid modification, substitution, and / or replacement, and a methionine to methionine oxide modification, substitution, and / or replacement. In some embodiments, the fibroin is a fibroin heavy chain, and wherein a modification, substitution, and / or replacement is at a position corresponding to any one position from 1 to 5263 of the fibroin heavy chain. In some embodiments, a modification, substitution, and / or replacement is at Q58, M64, N68, N70, N77, M80, N93, M103, Q125, N132, Q139, Q275, N4191, Q5216, and / or N5262 position of fibroin heavy chain. In some embodiments, the fibroin is a fibroin light chain, and wherein a modification, substitution, and / or DB1 / 155183601.2 2replacement is at a position corresponding to any one position from 1 to 262 of the fibroin light chain. In some embodiments, a modification, substitution, and / or replacement is at N23, Q24, N28, M69, N105, N108, N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, and / or Q255 position of fibroin light chain. In some embodiments, the fibroin is a fibrohexamerin (p25), and wherein a modification, substitution, and / or replacement is at a position corresponding to any one position from 1 to 220 of the fibrohexamerin (p25). In some embodiments, a modification, substitution, and / or replacement is at Q62, N93, M120, N149, N172, N174, and / or N202 position of fibrohexamerin (p25). The disclosure provides a composition comprising a plurality of peptides or protein fragments, each comprising a plurality of amino acids selected from M, R, V, K, T, F, I, L, C, A, Q, Y, N, D, E, G, S, H, P, and W, wherein at least one of the amino acids is modified, substituted, or replaced. In some embodiments, the plurality of peptides or protein fragments comprises a fibroin peptide or protein fragment comprising an amino acid modification, substitution, or replacement of an amino acid selected from M, R, V, K, T, F, I, L, C, A, Q, Y, N, D, E, G, S, H, P, and W. In some embodiments, the fibroin is a fibroin heavy chain, a fibroin light chain, or a fibrohexamerin. In some embodiments, the peptide or protein fragment comprises between about 2 and about 100 amino acids. In some embodiments, the peptide or protein fragment comprises between about 2 and about 25 amino acids. In some embodiments, the peptide or protein fragment comprises between about 25 and about 50 amino acids. In some embodiments, the peptide or protein fragment comprises between about 50 and about 75 amino acids. In some embodiments, the peptide or protein fragment comprises between about 75 and about 100 amino acids. In some embodiments, the peptide or protein fragment comprises between about 100 and about 125 amino acids. In some embodiments, the peptide or protein fragment comprises between about 125 and about 150 amino acids. In some embodiments, the peptide or protein fragment comprises between about 150 and about 200 amino acids. In some embodiments, the peptide or protein fragment comprises between about 200 and about 250, 300, 350, 400, 450, or 500 amino acids. In some embodiments, the peptide or protein fragment comprises between one and five modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises one modification, substitution, and / or replacement. In some embodiments, the peptide or protein fragment comprises two modifications, substitutions, and / or replacements. In DB1 / 155183601.2 3some embodiments, the peptide or protein fragment comprises three modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises four modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises five modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises six modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises seven modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises eight modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises nine modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises ten modifications, substitutions, and / or replacements. In some embodiments, a modification, substitution, and / or replacement is selected from an asparagine to aspartic acid modification, substitution, and / or replacement, a glutamine to glutamic acid modification, substitution, and / or replacement, and a methionine to methionine oxide modification, substitution, and / or replacement. In some embodiments, the fibroin is a fibroin heavy chain, and wherein a modification, substitution, and / or replacement is at a position corresponding to any one position from 1 to 5263 of the fibroin heavy chain. In some embodiments, a modification, substitution, and / or replacement is at Q58, M64, N68, N70, N77, M80, N93, M103, Q125, N132, Q139, Q275, N4191, Q5216, and / or N5262 position of fibroin heavy chain. In some embodiments, the fibroin is a fibroin light chain, and wherein a modification, substitution, and / or replacement is at a position corresponding to any one position from 1 to 262 of the fibroin light chain. In some embodiments, a modification, substitution, and / or replacement is at N23, Q24, N28, M69, N105, N108, N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, and / or Q255 position of fibroin light chain. In some embodiments, the fibroin is a fibrohexamerin (p25), and wherein a modification, substitution, and / or replacement is at a position corresponding to any one position from 1 to 220 of the fibrohexamerin (p25). In some embodiments, a modification, substitution, and / or replacement is at Q62, N93, M120, N149, N172, N174, and / or N202 position of fibrohexamerin (p25). In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 1% to about 99%. In some embodiments, each modification, substitution, and / or replacement is independently DB1 / 155183601.2 4ranging in the composition between about 1% to about 10%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 10% to about 20%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 20% to about 30%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 30% to about 40%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 40% to about 50%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 50% to about 60%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 60% to about 70%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 70% to about 80%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 80% to about 90%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between or about 90% to about 99%. As used herein, a % modification, substitution, and / or replacement is defined as (number of peptide or protein fragments comprising a modification, substitution, and / or replacement at a specific position, divided by the total number of peptide or protein fragments which include the specific position, whether comprising a modification, substitution, and / or replacement, or not) x 100. The disclosure provides a composition comprising a plurality of peptides or protein fragments of fibroin heavy chain, fibroin light chain, and / or fibrohexamerin (p25), the composition comprising one or more fractions, wherein the plurality of peptides or protein fragments comprises a fibroin peptide or protein fragment comprising an amino acid modification, substitution, or replacement. In some embodiments, the plurality of peptides or protein fragments having a weight average molecular weight (Mw) selected from between about 1 kDa and about 20 kDa, from between about 20 kDa and about 40 kDa, from between about 40 kDa and about 60 kDa, from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, from between about 100 kDa and about 120 kDa, from between about DB1 / 155183601.2 5120 kDa and about 140 kDa, or from between about 140 kDa and about 160 kDa, from between about 160 kDa and about 180 kDa, from between about 180 kDa and about 200 kDa, or from between about 200 kDa and about 250 kDa, and a polydispersity between 1 and about 1.7, between 1 and less than 3, or between 1 and less than 5. In some embodiments, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 10 kDa and about 20 kDa, from between about 20 kDa and about 40 kDa, from between about 40 kDa and about 60 kDa, from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, from between about 100 kDa and about 120 kDa, from between about 120 kDa and about 140 kDa, from between about 140 kDa and about 160 kDa, or from between about 160 kDa and about 180 kDa, and a polydispersity between 1 and about 1.1, or 1 and about 1.2, or between 1 and less than 3, or between 1 and less than 5. In some embodiments, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 10 kDa and about 20 kDa, from between about 20 kDa and about 40 kDa, from between about 40 kDa and about 60 kDa, from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, from between about 100 kDa and about 120 kDa, or from between about 120 kDa and about 140 kDa, and a polydispersity between 1 and about 1.1, or 1 and about 1.2, or between 1 and less than 3, or between 1 and less than 5. In some embodiments, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, or from between about 100 kDa and about 120 kDa, and a polydispersity between 1 and about 1.1, or between 1 and less than 3, or between 1 and less than 5. In some embodiments, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 10 kDa and about 20 kDa, from between about 20 kDa and about 40 kDa, from between about 40 kDa and about 60 kDa, from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, or from between about 100 kDa and about 110 kDa, and a polydispersity between 1 and about 1.1, or 1 and about 1.2, or between 1 and less than 3, or between 1 and less than 5. In some embodiments, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, from between about DB1 / 155183601.2 6100 kDa and about 120 kDa, or from between about 120 kDa and about 140 kDa, and a polydispersity between 1 and about 1.1, or between 1 and less than 3, or between 1 and less than 5. In some embodiments, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 20 kDa and about 40 kDa, or from between about 40 kDa and about 60 kDa, and a polydispersity between 1 and about 1.1, or 1 and about 1.2, or between 1 and less than 3, or between 1 and less than 5. In some embodiments, the one or more fractions are selected from AS77, AS78, AS79, AS80, and AS81. In some embodiments, the one or more fractions are selected from AS82, AS83, AS84, AS85, AS86, AS87, AS88, and AS89. In some embodiments, the one or more fractions are selected from AS90, AS91, AS92, AS93, and AS94. In some embodiments, the one or more fractions are selected from AS95, AS96, AS97, AS98, AS99, and AS100. In some embodiments, the plurality of peptides or protein fragments having a weight average molecular weight (Mw) selected from between about 40 kDa and about 60 kDa, from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, from between about 100 kDa and about 120 kDa, from between about 120 kDa and about 140 kDa, from between about 140 kDa and about 160 kDa, from between about 160 kDa and about 180 kDa, from between about 180 kDa and about 200 kDa, or from between about 200 kDa and about 220 kDa, and a polydispersity between 1 and about 1.7, or between 1 and less than 3, or between 1 and less than 5. In some embodiments, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 40 kDa and about 60 kDa, from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, from between about 100 kDa and about 120 kDa, from between about 120 kDa and about 140 kDa, from between about 140 kDa and about 160 kDa, from between about 160 kDa and about 180 kDa, from between about 180 kDa and about 200 kDa, or from between about 200 kDa and about 210 kDa, and a polydispersity between 1 and about 1.2, or 1 and about 1.3, or between 1 and less than 3, or between 1 and less than 5. In some embodiments, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 40 kDa and about 60 kDa, from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, or from between about 100 kDa and about 110 kDa, and a polydispersity between 1 and about 1.1, or 1 and about 1.2, or between 1 and less than 3, or between 1 and less than 5. In some DB1 / 155183601.2 7embodiments, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, from between about 100 kDa and about 120 kDa, from between about 120 kDa and about 140 kDa, from between about 140 kDa and about 160 kDa, from between about 160 kDa and about 180 kDa, from between about 180 kDa and about 200 kDa, or from between about 200 kDa and about 210 kDa, and a polydispersity between 1 and about 1.2, or 1 and about 1.3, or between 1 and less than 3, or between 1 and less than 5. In some embodiments, the one or more fractions are selected from AS101, AS102, AS103, AS104, and AS105. In some embodiments, the one or more fractions are selected from AS106, AS107, AS108, AS109, AS110, and AS111. In some embodiments, an amino acid is selected from M, R, V, K, T, F, I, L, C, A, Q, Y, N, D, E, G, S, H, P, and W. In some embodiments, a peptide or protein fragment comprises between about 2 and about 100 amino acids. In some embodiments, the peptide or protein fragment comprises between about 2 and about 25 amino acids. In some embodiments, the peptide or protein fragment comprises between about 25 and about 50 amino acids. In some embodiments, the peptide or protein fragment comprises between about 50 and about 75 amino acids. In some embodiments, the peptide or protein fragment comprises between about 75 and about 100 amino acids. In some embodiments, the peptide or protein fragment comprises between about 100 and about 125 amino acids. In some embodiments, the peptide or protein fragment comprises between about 125 and about 150 amino acids. In some embodiments, the peptide or protein fragment comprises between about 150 and about 200 amino acids. In some embodiments, the peptide or protein fragment comprises between about 200 and about 250, 300, 350, 400, 450, or 500 amino acids. In some embodiments, a peptide or protein fragment comprises between one and five modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises one modification, substitution, and / or replacement. In some embodiments, the peptide or protein fragment comprises two modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises three modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises four modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises five modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein DB1 / 155183601.2 8fragment comprises six modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises seven modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises eight modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises nine modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises ten modifications, substitutions, and / or replacements. In some embodiments, the fibroin is a fibroin heavy chain, and wherein a modification, substitution, and / or replacement is at a position corresponding to any one position from 1 to 5263 of the fibroin heavy chain. In some embodiments, the fibroin is a fibroin light chain, and wherein a modification, substitution, and / or replacement is at a position corresponding to any one position from 1 to 262 of the fibroin light chain. In some embodiments, the fibroin is a fibrohexamerin (p25) chain, and wherein a modification, substitution, and / or replacement is at a position corresponding to any one position from 1 to 220 of the fibrohexamerin (p25) chain. In some embodiments, a modification, substitution, and / or replacement is selected from an asparagine to aspartic acid modification, substitution, and / or replacement, a glutamine to glutamic acid modification, substitution, and / or replacement, and a methionine to methionine oxide modification, substitution, and / or replacement. In some embodiments, a modification, substitution, and / or replacement is at fibroin heavy chain position selected from Q58, M64, N68, N70, N77, M80, N93, M103, Q125, N132, Q139, Q275, N4191, Q5216, and / or N5262. In some embodiments, a modification, substitution, and / or replacement is at fibroin light chain position selected from N23, Q24, N28, M69, N105, N108, N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, and / or Q255. In some embodiments, a modification, substitution, and / or replacement is at fibrohexamerin (p25) position selected from Q62, N93, M120, N149, N172, N174, and / or N202. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 1% to about 99%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 1% to about 10%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 10% to about 20%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 20% to about 30%. In some DB1 / 155183601.2 9embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 30% to about 40%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 40% to about 50%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 50% to about 60%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 60% to about 70%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 70% to about 80%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 80% to about 90%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between or about 90% to about 99%. As used herein, a % modification, substitution, and / or replacement is defined as (number of peptide or protein fragments comprising a modification, substitution, and / or replacement at a specific position, divided by the total number of peptide or protein fragments which include the specific position, whether comprising a modification, substitution, and / or replacement, or not) x 100. In some embodiments, a molecular weight is determined by MALS. The disclosure provides an article comprising one or more peptides or protein fragments disclosed herein, and / or one or more compositions disclosed herein. A composition may include unmodified fibroin peptides and protein fragments. An article is, without limitation, selected from a personal care article disclosed herein, a coated fabric disclosed herein, and / or an article for treating fabrics (e.g., laundry pod) disclosed herein. The disclosure provides an article comprising one or more peptides or protein fragments disclosed herein, and / or one or more compositions disclosed herein, and a substrate or any other support or combination element disclosed herein. In some embodiments, the disclosure provides a composition disclosed herein comprising a plurality of peptides or protein fragments, e.g., a plurality of fibroin heavy chain peptides or fibroin heavy chain fragments, and comprising a plurality of amino acid modifications, substitutions, and / or replacements, and further comprising one or more ratios of modifications, substitutions, and / or replacements at specific positions selected from: DB1 / 155183601.2 10- a ratio of Q58 to M64 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q58 to N68 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q58 to N70 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q58 to N77 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q58 to M80 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q58 to N93 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q58 to M103 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about DB1 / 155183601.2 111:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q58 to Q125 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q58 to N132 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q58 to Q139 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q58 to Q275 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q58 to N4191 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q58 to Q5216 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q58 to N5262 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about DB1 / 155183601.2 126:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M64 to N68 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M64 to N70 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M64 to N77 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M64 to M80 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M64 to N93 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M64 to M103 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; DB1 / 155183601.2 13- a ratio of M64 to Q125 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M64 to N132 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M64 to Q139 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M64 to Q275 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M64 to N4191 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M64 to Q5216 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M64 to N5262 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about DB1 / 155183601.2 141:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N68 to N70 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N68 to N77 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N68 to M80 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N68 to N93 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N68 to M103 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N68 to Q125 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N68 to N132 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about DB1 / 155183601.2 156:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N68 to Q139 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N68 to Q275 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N68 to N4191 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N68 to Q5216 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N68 to N5262 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N70 to N77 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; DB1 / 155183601.2 16- a ratio of N70 to M80 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N70 to N93 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N70 to M103 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N70 to Q125 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N70 to N132 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N70 to Q139 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N70 to Q275 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about DB1 / 155183601.2 171:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N70 to N4191 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N70 to Q5216 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N70 to N5262 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N77 to M80 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N77 to N93 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N77 to M103 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N77 to Q125 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about DB1 / 155183601.2 186:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N77 to N132 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N77 to Q139 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N77 to Q275 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N77 to N4191 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N77 to Q5216 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N77 to N5262 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; DB1 / 155183601.2 19- a ratio of M80 to N93 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M80 to M103 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M80 to Q125 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M80 to N132 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M80 to Q139 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M80 to Q275 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M80 to N4191 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about DB1 / 155183601.2 201:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M80 to Q5216 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M80 to N5262 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N93 to M103 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N93 to Q125 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N93 to N132 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N93 to Q139 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N93 to Q275 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about DB1 / 155183601.2 216:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N93 to N4191 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N93 to Q5216 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N93 to N5262 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M103 to Q125 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M103 to N132 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M103 to Q139 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; DB1 / 155183601.2 22- a ratio of M103 to Q275 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M103 to N4191 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M103 to Q5216 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M103 to N5262 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q125 to N132 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q125 to Q139 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q125 to Q275 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about DB1 / 155183601.2 231:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q125 to N4191 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q125 to Q5216 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q125 to N5262 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N132 to Q139 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N132 to Q275 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N132 to N4191 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N132 to Q5216 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about DB1 / 155183601.2 246:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N132 to N5262 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q139 to Q275 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q139 to N4191 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q139 to Q5216 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q139 to N5262 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q275 to N4191 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; DB1 / 155183601.2 25- a ratio of Q275 to Q5216 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q275 to N5262 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N4191 to Q5216 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N4191 to N5262 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q5216 to N5262 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; In some embodiments, the disclosure provides a composition comprising a plurality of peptides or protein fragments, e.g., a plurality of fibroin heavy chain and light chain peptides or fibroin heavy chain and light chain fragments, and comprising a plurality of amino acid modifications, substitutions, and / or replacements, and further comprising one or more ratios of modifications, substitutions, and / or replacements at specific positions selected from: -a ratio of heavy chain Q58 to light chain N23, Q24, N28, M69, N105, N108,N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about DB1 / 155183601.2 2615:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of heavy chain M64 to light chain N23, Q24, N28, M69, N105, N108,N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of heavy chain N68 to light chain N23, Q24, N28, M69, N105, N108,N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of heavy chain N70 to light chain N23, Q24, N28, M69, N105, N108,N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of heavy chain N77 to light chain N23, Q24, N28, M69, N105, N108,N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of heavy chain M80 to light chain N23, Q24, N28, M69, N105, N108,N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; DB1 / 155183601.2 27- a ratio of heavy chain N93 to light chain N23, Q24, N28, M69, N105, N108,N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of heavy chain M103 to light chain N23, Q24, N28, M69, N105, N108,N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of heavy chain Q125 to light chain N23, Q24, N28, M69, N105, N108,N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of heavy chain N132 to light chain N23, Q24, N28, M69, N105, N108,N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of heavy chain Q139 to light chain N23, Q24, N28, M69, N105, N108,N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of heavy chain Q275 to light chain N23, Q24, N28, M69, N105, N108,N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, DB1 / 155183601.2 28about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of heavy chain N4191 to light chain N23, Q24, N28, M69, N105,N108, N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of heavy chain Q5216 to light chain N23, Q24, N28, M69, N105,N108, N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of heavy chain N5262 to light chain N23, Q24, N28, M69, N105,N108, N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25. In some embodiments, the disclosure provides a composition comprising a plurality of peptides or protein fragments, e.g., a plurality of fibroin heavy chain and p25 peptides or fibroin heavy chain and p25 fragments, and comprising a plurality of amino acid modifications, substitutions, and / or replacements, comprising one or more ratios of modifications, substitutions, and / or replacements at specific positions selected from: -a ratio of heavy chain Q58 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of heavy chain M64 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about DB1 / 155183601.2 294:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of heavy chain N68 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of heavy chain N70 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of heavy chain N77 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of heavy chain M80 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of heavy chain N93 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of heavy chain M103 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of heavy chain Q125 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, DB1 / 155183601.2 30about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of heavy chain N132 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of heavy chain Q139 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of heavy chain Q275 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of heavy chain N4191 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of heavy chain Q5216 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of heavy chain N5262 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25. DB1 / 155183601.2 31In some embodiments, the disclosure provides a composition comprising a plurality of peptides or protein fragments, e.g., a plurality of light chain fibroin peptides or fibroin fragments, and comprising a plurality of amino acid modifications, substitutions, and / or replacements, comprising one or more ratios of modifications, substitutions, and / or replacements at specific positions selected from: -a ratio of N23 to Q24 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q24 to N28 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q24 to M69 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q24 to N105 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q24 to N108 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q24 to N118 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about DB1 / 155183601.2 321:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q24 to N136 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q24 to N138 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q24 to Q149 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q24 to N186 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q24 to N200 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q24 to Q202 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q24 to N204 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about DB1 / 155183601.2 336:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q24 to N240 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q24 to N248 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q24 to Q255 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N28 to M69 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N28 to N105 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N28 to N108 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; DB1 / 155183601.2 34- a ratio of N28 to N118 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N28 to N136 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N28 to N138 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N28 to Q149 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N28 to N186 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N28 to N200 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N28 to Q202 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about DB1 / 155183601.2 351:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N28 to N204 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N28 to N240 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N28 to N248 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N28 to Q255 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M69 to N105 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M69 to N108 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M69 to N118 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about DB1 / 155183601.2 366:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M69 to N136 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M69 to N138 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M69 to Q149 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M69 to N186 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M69 to N200 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M69 to Q202 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; DB1 / 155183601.2 37- a ratio of M69 to N204 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M69 to N240 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M69 to N248 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M69 to Q255 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N105 to N108 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N105 to N118 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N105 to N136 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about DB1 / 155183601.2 381:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N105 to N138 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N105 to Q149 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N105 to N186 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N105 to N200 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N105 to Q202 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N105 to N204 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N105 to N240 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about DB1 / 155183601.2 396:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N105 to N248 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N105 to Q255 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N108 to N118 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N108 to N136 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N108 to N138 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N108 to Q149 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; DB1 / 155183601.2 40- a ratio of N108 to N186 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N108 to N200 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N108 to Q202 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N108 to N204 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N108 to N240 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N108 to N248 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N108 to Q255 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about DB1 / 155183601.2 411:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N118 to N136 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N118 to N138 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N118 to Q149 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N118 to N186 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N118 to N200 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N118 to Q202 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N118 to N204 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about DB1 / 155183601.2 426:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N118 to N240 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N118 to N248 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N118 to Q255 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N136 to N138 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N136 to Q149 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N136 to N186 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; DB1 / 155183601.2 43- a ratio of N136 to N200 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N136 to Q202 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N136 to N204 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N136 to N240 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N136 to N248 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N136 to Q255 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N138 to Q149 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about DB1 / 155183601.2 441:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N138 to N186 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N138 to N200 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N138 to Q202 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N138 to N204 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N138 to N240 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N138 to N248 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N138 to Q255 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about DB1 / 155183601.2 456:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q149 to N186 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q149 to N200 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q149 to Q202 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q149 to N204 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q149 to N240 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q149 to N248 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; DB1 / 155183601.2 46- a ratio of Q149 to Q255 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N186 to N200 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N186 to Q202 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N186 to N204 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N186 to N240 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N186 to N248 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N186 to Q255 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about DB1 / 155183601.2 471:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N200 to Q202 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N200 to N204 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N200 to N240 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N200 to N248 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N200 to Q255 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q202 to N204 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q202 to N240 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about DB1 / 155183601.2 486:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q202 to N248 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q202 to Q255 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N204 to N240 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N204 to N248 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N204 to Q255 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N240 to N248 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; DB1 / 155183601.2 49- a ratio of N240 to Q255 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N248 to Q255 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; In some embodiments, the disclosure provides a composition comprising a plurality of peptides or protein fragments, e.g., a plurality of fibroin light chain and heavy chain peptides or fibroin light chain and heavy chain fragments, and comprising a plurality of amino acid modifications, substitutions, and / or replacements, and further comprising one or more ratios of modifications, substitutions, and / or replacements at specific positions selected from: -a ratio of light chain N23 to heavy chain Q58, M64, N68, N70, N77, M80,N93, M103, Q125, N132, Q139, Q275, N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of light chain Q24 to heavy chain Q58, M64, N68, N70, N77, M80,N93, M103, Q125, N132, Q139, Q275, N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of light chain N28 to heavy chain Q58, M64, N68, N70, N77, M80,N93, M103, Q125, N132, Q139, Q275, N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; DB1 / 155183601.2 50- a ratio of light chain M69 to heavy chain Q58, M64, N68, N70, N77, M80,N93, M103, Q125, N132, Q139, Q275, N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of light chain N105 to heavy chain Q58, M64, N68, N70, N77, M80,N93, M103, Q125, N132, Q139, Q275, N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of light chain N108 to heavy chain Q58, M64, N68, N70, N77, M80,N93, M103, Q125, N132, Q139, Q275, N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of light chain N118 to heavy chain Q58, M64, N68, N70, N77, M80,N93, M103, Q125, N132, Q139, Q275, N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of light chain N136 to heavy chain Q58, M64, N68, N70, N77, M80,N93, M103, Q125, N132, Q139, Q275, N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of light chain N138 to heavy chain Q58, M64, N68, N70, N77, M80,N93, M103, Q125, N132, Q139, Q275, N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, DB1 / 155183601.2 51about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of light chain Q149 to heavy chain Q58, M64, N68, N70, N77, M80,N93, M103, Q125, N132, Q139, Q275, N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of light chain N186 to heavy chain Q58, M64, N68, N70, N77, M80,N93, M103, Q125, N132, Q139, Q275, N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of light chain N200 to heavy chain Q58, M64, N68, N70, N77, M80,N93, M103, Q125, N132, Q139, Q275, N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of light chain Q202 to heavy chain Q58, M64, N68, N70, N77, M80,N93, M103, Q125, N132, Q139, Q275, N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of light chain N204 to heavy chain Q58, M64, N68, N70, N77, M80,N93, M103, Q125, N132, Q139, Q275, N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of light chain N240 to heavy chain Q58, M64, N68, N70, N77, M80,N93, M103, Q125, N132, Q139, Q275, N4191, Q5216, or N5262 modifications, DB1 / 155183601.2 52substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of light chain N248 to heavy chain Q58, M64, N68, N70, N77, M80,N93, M103, Q125, N132, Q139, Q275, N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of light chain Q255 to heavy chain Q58, M64, N68, N70, N77, M80,N93, M103, Q125, N132, Q139, Q275, N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25. In some embodiments, the disclosure provides a composition comprising a plurality of peptides or protein fragments, e.g., a plurality of fibroin light chain and p25 peptides or fibroin light chain and p25 fragments, and comprising a plurality of amino acid modifications, substitutions, and / or replacements, comprising one or more ratios of modifications, substitutions, and / or replacements at specific positions selected from: -a ratio of light chain N23 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of light chain Q24 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of light chain N28 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, DB1 / 155183601.2 53about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of light chain M69 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of light chain N105 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of light chain N108 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of light chain N118 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of light chain N136 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of light chain N138 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; DB1 / 155183601.2 54- a ratio of light chain Q149 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of light chain N186 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of light chain N200 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of light chain Q202 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of light chain N204 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of light chain N240 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of light chain N248 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about DB1 / 155183601.2 554:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of light chain Q255 to p25 Q62, N93, M120, N149, N172, N174, orN202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25. In some embodiments, the disclosure provides a composition comprising a plurality of peptides or protein fragments, e.g., a plurality of p25 fibroin peptides or fibroin fragments, and comprising a plurality of amino acid modifications, substitutions, and / or replacements, comprising one or more ratios of modifications, substitutions, and / or replacements at specific positions selected from: -a ratio of Q62 to N93 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q62 to M120 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q62 to N149 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q62 to N172 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q62 to N174 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about DB1 / 155183601.2 566:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of Q62 to N202 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N93 to M120 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N93 to N149 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N93 to N172 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N93 to N174 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N93 to N202 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; DB1 / 155183601.2 57- a ratio of M120 to N149 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M120 to N172 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M120 to N174 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of M120 to N202 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N149 to N172 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N149 to N174 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N149 to N202 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about DB1 / 155183601.2 581:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N172 to N174 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N172 to N202 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of N174 to N202 modifications, substitutions, and / or replacements ofabout 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25. In some embodiments, the disclosure provides a composition comprising a plurality of peptides or protein fragments, e.g., a plurality of p25 and heavy chain peptides or p25 and heavy chain fragments, and comprising a plurality of amino acid modifications, substitutions, and / or replacements, comprising one or more ratios of modifications, substitutions, and / or replacements at specific positions selected from: -a ratio of p25 Q62 to heavy chain Q58, M64, N68, N70, N77, M80, N93,M103, Q125, N132, Q139, Q275, N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of p25 N93 to heavy chain Q58, M64, N68, N70, N77, M80, N93,M103, Q125, N132, Q139, Q275, N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; DB1 / 155183601.2 59- a ratio of p25 M120 to heavy chain Q58, M64, N68, N70, N77, M80, N93,M103, Q125, N132, Q139, Q275, N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of p25 N149 to heavy chain Q58, M64, N68, N70, N77, M80, N93,M103, Q125, N132, Q139, Q275, N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of p25 N172 to heavy chain Q58, M64, N68, N70, N77, M80, N93,M103, Q125, N132, Q139, Q275, N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of p25 N174 to heavy chain Q58, M64, N68, N70, N77, M80, N93,M103, Q125, N132, Q139, Q275, N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of p25 N202 to heavy chain Q58, M64, N68, N70, N77, M80, N93,M103, Q125, N132, Q139, Q275, N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25. In some embodiments, the disclosure provides a composition comprising a plurality of peptides or protein fragments, e.g., a plurality of p25 and light chain peptides or fibroin light chain and light chain fragments, and comprising a plurality of amino acid modifications, substitutions, and / or replacements, and further comprising DB1 / 155183601.2 60one or more ratios of modifications, substitutions, and / or replacements at specific positions selected from: -a ratio of p25 Q62 to light chain N23, Q24, N28, M69, N105, N108, N118,N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of p25 N93 to light chain N23, Q24, N28, M69, N105, N108, N118,N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of p25 M120 to light chain N23, Q24, N28, M69, N105, N108, N118,N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of p25 N149 to light chain N23, Q24, N28, M69, N105, N108, N118,N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of p25 N172 to light chain N23, Q24, N28, M69, N105, N108, N118,N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of p25 N174 to light chain N23, Q24, N28, M69, N105, N108, N118,N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, DB1 / 155183601.2 61substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; -a ratio of p25 N202 to light chain N23, Q24, N28, M69, N105, N108, N118,N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25. The disclosure provides an article comprising a fabric and a coating, wherein the coating comprises a surfactant and / or emulsifier system, and silk fibroin fragments having an average weight average molecular weight selected from between about 1 kDa and about 5 kDa, from between about 5 kDa and about 10 kDa, from between about 6 kDa and about 17 kDa, from between about 10 kDa and about 15 kDa, from between about 14 kDa and about 30 kDa, from between about 15 kDa and about 20 kDa, from between about 17 kDa and about 39 kDa, from between about 20 kDa and about 25 kDa, from between about 25 kDa and about 30 kDa, from between about 30 kDa and about 35 kDa, from between about 35 kDa and about 40 kDa, from between about 39 kDa and about 54 kDa, from between about 39 kDa and about 80 kDa, from between about 40 kDa and about 45 kDa, from between about 45 kDa and about 50 kDa, from between about 50 kDa and about 55 kDa, from between about 55 kDa and about 60 kDa, from between about 60 kDa and about 100 kDa, or from between about 80 kDa and about 144 kDa, and a polydispersity ranging from 1 to about 5. In some embodiments, the coating comprises fibroin peptides and / or fibroin fragments disclosed herein, e.g., and without limitation, fibroin peptides and / or protein fragments wherein at least one of the amino acids is modified, substituted, or replaced as disclosed herein. In some embodiments, the silk fibroin fragments have a polydispersity from 1 to about 1.5, from about 1.5 to about 2.0, from about 2.0 to about 2.5, from about 2.5 to about 3.0, from about 3.0 to about 3.5, from about 3.5 to about 4.0, from about 4.0 to about 4.5, or from about 4.5 to about 5.0. In some embodiments, the silk fibroin fragments have a polydispersity from about 1.5 to about 3.0. In some embodiments, the silk fibroin fragments comprise one or more of low molecular weight silk fibroin fragments and medium molecular weight silk fibroin DB1 / 155183601.2 62fragments. In some embodiments, the article further comprises about 0.01% (w / w) to about 10% (w / w) sericin relative to the silk fibroin fragments. In some embodiments, the fabric comprises one or more of polyester, polyamide, polyaramid, polytetrafluoroethylene, polyethylene, polypropylene, polyurethane, silicone, mixtures of polyurethane and polyethyleneglycol, ultrahigh molecular weight polyethylene, high-performance polyethylene, nylon, LYCRA (polyester- polyurethane copolymer, also known as SPANDEX and elastomer), or a mixture thereof. In some embodiments, the coating further comprises one or more of a wetting agent, an anti-foaming agent, a softener, a wicking agent, and an anti-microbial. In some embodiments, the w / w ratio of silk fibroin fragments to the surfactant and / or emulsifier system in the coating is about 99:1, about 98:2, about 97:3, about 96:4, about 95:5, about 94:6, about 93:7, about 92:8, about 91:9, about 90:10, about 89:11, about 88:12, about 87:13, about 86:14, about 85:15, about 84:16, about 83:17, about 82:18, about 81:19, about 80:20, about 79:21, about 78:22, about 77:23, about 76:24, about 75:25, about 74:26, about 73:27, about 72:28, about 71:29, about 70:30, about 69:31, about 68:32, about 67:33, about 66:34, about 65:35, about 64:36, about 63:37, about 62:38, about 61:39, about 60:40, about 59:41, about 58:42, about 57:43, about 56:44, about 55:45, about 54:46, about 53:47, about 52:48, about 51:49, about 50:50, about 49:51, about 48:52, about 47:53, about 46:54, about 45:55, about 44:56, about 43:57, about 42:58, about 41:59, about 40:60, about 39:61, about 38:62, about 37:63, about 36:64, about 35:65, about 34:66, about 33:67, about 32:68, about 31:69, about 30:70, about 29:71, about 28:72, about 27:73, about 26:74, about 25:75, about 24:76, about 23:77, about 22:78, about 21:79, about 20:80, about 19:81, about 18:82, about 17:83, about 16:84, about 15:85, about 14:86, about 13:87, about 12:88, about 11:89, about 10:90, about 9:91, about 8:92, about 7:93, about 6:94, about 5:95, about 4:96, about 3:97, about 2:98, or about 1:99. In some embodiments, the w / w ratio of silk fibroin fragments to the surfactant and / or emulsifier in the coating is about 1:1, about 1:2, about 1:4, about 1:8, about 1:16, or about 1:32. In some embodiments, the w / w ratio of silk fibroin fragments to the surfactant and / or emulsifier system in the coating is about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:11, about 1:12, about 1:13, about 1:14, about 1:15, about 1:16, about 1:17, about 1:18, about 1:19, about 1:20, about 1:21, about 1:22, about 1:23, about 1:24, about 1:25, about 1:26, about 1:27, about 1:28, about 1:29, about 1:30, about 1:31, or about 1:32. In some embodiments, the surfactant and / or DB1 / 155183601.2 63emulsifier system comprises one or more of polyoxyethylene sorbitan monooleate, polyoxyethylene sorbitan trioleate, polyoxyethylene castor oil, and any combination thereof. In some embodiments, the surfactant and / or emulsifier system comprises one or more of polyoxyethylene (10-30) sorbitan monooleate, polyoxyethylene (10-30) sorbitan trioleate, polyoxyethylene (10-50) castor oil, and any combination thereof. In some embodiments, the surfactant and / or emulsifier system comprises one or more of polyoxyethylene (20) sorbitan monooleate, polyoxyethylene (20) sorbitan trioleate, polyoxyethylene (29) castor oil, and any combination thereof. In some embodiments, the surfactant and / or emulsifier system comprises one or more of polyoxyethylene (20) sorbitan monolaurate, polyoxyethylene (20) sorbitan monopalmitate, polyoxyethylene (20) sorbitan monostearate, polyoxyethylene (20) sorbitan tristearate, and any combination thereof. In some embodiments, the surfactant and / or emulsifier system comprises one or more of a sorbitan mono fatty acid, a sorbitan tri fatty acid, a castor oil, and any combination thereof. In some embodiments, the surfactant and / or emulsifier system comprises one or more of coco glucoside, decyl glucoside, lauryl glucoside, sucrose cocoate, capryl / caprylyl glucoside, caprylyl / capryl glucoside, and any combination thereof. In some embodiments, the surfactant and / or emulsifier system has an HLB between about 11 and about 13.50. In some embodiments, the surfactant and / or emulsifier system has an HLB between about 11 and about 11.50, between about 11.50 and about 12, between about 12 and about 12.50, between about 12.50 and about 13, or between about 13 and about 13.50. In some embodiments, the article has an improved moisture management comparative to a similar article comprising a similar fabric but no coating. In some embodiments, moisture management is assessed by a water absorbency test, a vertical wicking test, or a dry rate test. In some embodiments, the article has an improved drapability comparative to a similar article comprising a similar fabric but no coating. In some embodiments, the article has an improved smoothness comparative to a similar article comprising a similar fabric but no coating. In some embodiments, the article has an improved hand feel comparative to a similar article comprising a similar fabric but no coating. In some embodiments, the article has a lower charge density at a given pH value comparative to a similar article comprising a similar fabric but no coating. The disclosure provides a method of making a silk fibroin coated fabric, comprising: applying to the fabric a solution comprising a surfactant and / or emulsifier system; applying to the fabric a silk fibroin fragments solution; and drying the fabric. DB1 / 155183601.2 64In some embodiments, the method comprises the use of modified silk fragments disclosed herein, e.g., and without limitation, fibroin peptides and / or protein fragments wherein at least one of the amino acids is modified, substituted, or replaced as disclosed herein. The disclosure also provides a method of making a silk fibroin coated fabric, comprising: applying to the fabric a solution comprising a surfactant and / or emulsifier system and silk fibroin fragments; and drying the fabric. In some embodiments, the concentration of the silk fibroin fragments in a solution ranges from 0.01 g / L to about 100 g / L. In some embodiments, the concentration of the surfactant and / or emulsifier system in a solution ranges from 0.01 g / L to about 100 g / L. In some embodiments, the silk fibroin fragments have an average weight average molecular weight selected from between about 1 kDa and about 5 kDa, from between about 5 kDa and about 10 kDa, from between about 6 kDa and about 17 kDa, from between about 10 kDa and about 15 kDa, from between about 14 kDa and about 30 kDa, from between about 15 kDa and about 20 kDa, from between about 17 kDa and about 39 kDa, from between about 20 kDa and about 25 kDa, from between about 25 kDa and about 30 kDa, from between about 30 kDa and about 35 kDa, from between about 35 kDa and about 40 kDa, from between about 39 kDa and about 54 kDa, from between about 39 kDa and about 80 kDa, from between about 40 kDa and about 45 kDa, from between about 45 kDa and about 50 kDa, from between about 50 kDa and about 55 kDa, from between about 55 kDa and about 60 kDa, from between about 60 kDa and about 100 kDa, or from between about 80 kDa and about 144 kDa, and a polydispersity ranging from 1 to about 5. In some embodiments, the silk fibroin fragments have a polydispersity from 1 to about 1.5, from about 1.5 to about 2.0, from about 2.0 to about 2.5, from about 2.5 to about 3.0, from about 3.0 to about 3.5, from about 3.5 to about 4.0, from about 4.0 to about 4.5, or from about 4.5 to about 5.0. In some embodiments, the silk fibroin fragments have a polydispersity from about 1.5 to about 3.0. In some embodiments, the silk fibroin fragments comprise one or more of low molecular weight silk fibroin fragments and medium molecular weight silk fibroin fragments. In some embodiments, a solution further comprises about 0.01% (w / w) to about 10% (w / w) sericin relative to the silk fibroin fragments. In some embodiments, the fabric comprises one or more of polyester, polyamide, polyaramid, polytetrafluoroethylene, polyethylene, polypropylene, polyurethane, silicone, mixtures of polyurethane and polyethyleneglycol, ultrahigh molecular weight polyethylene, high-performance polyethylene, nylon, LYCRA (polyester- DB1 / 155183601.2 65polyurethane copolymer, also known as SPANDEX and elastomer), or a mixture thereof. In some embodiments, a solution further comprises one or more of a wetting agent, an anti-foaming agent, a softener, a wicking agent, and an anti-microbial. In some embodiments, the w / w ratio of silk fibroin fragments to the surfactant and / or emulsifier system is about 99:1, about 98:2, about 97:3, about 96:4, about 95:5, about 94:6, about 93:7, about 92:8, about 91:9, about 90:10, about 89:11, about 88:12, about 87:13, about 86:14, about 85:15, about 84:16, about 83:17, about 82:18, about 81:19, about 80:20, about 79:21, about 78:22, about 77:23, about 76:24, about 75:25, about 74:26, about 73:27, about 72:28, about 71:29, about 70:30, about 69:31, about 68:32, about 67:33, about 66:34, about 65:35, about 64:36, about 63:37, about 62:38, about 61:39, about 60:40, about 59:41, about 58:42, about 57:43, about 56:44, about 55:45, about 54:46, about 53:47, about 52:48, about 51:49, about 50:50, about 49:51, about 48:52, about 47:53, about 46:54, about 45:55, about 44:56, about 43:57, about 42:58, about 41:59, about 40:60, about 39:61, about 38:62, about 37:63, about 36:64, about 35:65, about 34:66, about 33:67, about 32:68, about 31:69, about 30:70, about 29:71, about 28:72, about 27:73, about 26:74, about 25:75, about 24:76, about 23:77, about 22:78, about 21:79, about 20:80, about 19:81, about 18:82, about 17:83, about 16:84, about 15:85, about 14:86, about 13:87, about 12:88, about 11:89, about 10:90, about 9:91, about 8:92, about 7:93, about 6:94, about 5:95, about 4:96, about 3:97, about 2:98, or about 1:99. In some embodiments, the w / w ratio of silk fibroin fragments to the surfactant and / or emulsifier system is about 1:1, about 1:2, about 1:4, about 1:8, about 1:16, or about 1:32. In some embodiments, the w / w ratio of silk fibroin fragments to the surfactant and / or emulsifier system is about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:11, about 1:12, about 1:13, about 1:14, about 1:15, about 1:16, about 1:17, about 1:18, about 1:19, about 1:20, about 1:21, about 1:22, about 1:23, about 1:24, about 1:25, about 1:26, about 1:27, about 1:28, about 1:29, about 1:30, about 1:31, or about 1:32. In some embodiments, the surfactant and / or emulsifier system comprises one or more of polyoxyethylene sorbitan monooleate, polyoxyethylene sorbitan trioleate, polyoxyethylene castor oil, and any combination thereof. In some embodiments, the surfactant and / or emulsifier system comprises one or more of polyoxyethylene (10- 30) sorbitan monooleate, polyoxyethylene (10-30) sorbitan trioleate, polyoxyethylene (10-50) castor oil, and any combination thereof. In some embodiments, the surfactant and / or emulsifier system comprises one or more of polyoxyethylene (20) sorbitan DB1 / 155183601.2 66monooleate, polyoxyethylene (20) sorbitan trioleate, polyoxyethylene (29) castor oil, and any combination thereof. In some embodiments, the surfactant and / or emulsifier system comprises one or more of polyoxyethylene (20) sorbitan monolaurate, polyoxyethylene (20) sorbitan monopalmitate, polyoxyethylene (20) sorbitan monostearate, polyoxyethylene (20) sorbitan tristearate, and any combination thereof. In some embodiments, the surfactant and / or emulsifier system comprises one or more of a sorbitan mono fatty acid, a sorbitan tri fatty acid, a castor oil, and any combination thereof. In some embodiments, the surfactant and / or emulsifier system comprises one or more of coco glucoside, decyl glucoside, lauryl glucoside, sucrose cocoate, capryl / caprylyl glucoside, caprylyl / capryl glucoside, and any combination thereof. In some embodiments, the surfactant and / or emulsifier system has an HLB between about 11 and about 13.50. In some embodiments, the surfactant and / or emulsifier system has an HLB between about 11 and about 11.50, between about 11.50 and about 12, between about 12 and about 12.50, between about 12.50 and about 13, or between about 13 and about 13.50. In some embodiments, the drying comprises heating. In some embodiments, the pH of a solution is acidic. In some embodiments, the pH of a solution is between about 3.5 and about 4, between about 4 and about 4.5, between about 4.5 and about 5, between about 5 and about 5.5, or between about 5.5 and about 6. The disclosure also provides an article prepared by a method described herein, including, without limitation, by using a modified silk fragment disclosed herein, e.g., and without limitation, fibroin peptides and / or protein fragments wherein at least one of the amino acids is modified, substituted, or replaced as disclosed herein. In some embodiments, the article has an improved moisture management comparative to a similar article comprising a similar fabric but no coating. In some embodiments, moisture management is assessed by a water absorbency test, a vertical wicking test, or a dry rate test. In some embodiments, the article has an improved drapability comparative to a similar article comprising a similar fabric but no coating. In some embodiments, the article has an improved smoothness comparative to a similar article comprising a similar fabric but no coating. In some embodiments, the article has an improved hand feel comparative to a similar article comprising a similar fabric but no coating. In some embodiments, the article has a lower charge density at a given pH value comparative to a similar article comprising a similar fabric but no coating. DB1 / 155183601.2 67In some embodiments, the disclosure further provides personal care and / or cosmetic formulations and / or compositions comprising silk fibroin fragments having an average weight average molecular weight selected from between about 1 kDa and about 5 kDa, between about 5 kDa and about 10 kDa, between about 6 kDa and about 17 kDa, between about 10 kDa and about 15 kDa, between about 15 kDa and about 20 kDa, between about 14 kDa and about 30 kDa, between about 17 kDa and about 39 kDa, between about 20 kDa and about 25 kDa, between about 25 kDa and about 30 kDa, between about 30 kDa and about 35 kDa, between about 35 kDa and about 40 kDa, between about 39 kDa and about 54 kDa, between about 39 kDa and about 80 kDa, between about 40 kDa and about 45 kDa, between about 45 kDa and about 50 kDa, between about 60 kDa and about 100 kDa, and between about 80 kDa and about 144 kDa, and a polydispersity between 1 and about 5, and without limitation, can also comprise modified silk fragments disclosed herein, e.g., and without limitation, fibroin peptides and / or protein fragments wherein at least one of the amino acids is modified, substituted, or replaced as disclosed herein. In some embodiments, the composition further comprises 0 to 500 ppm lithium bromide. In some embodiments, the composition further comprises 0 to 500 ppm sodium carbonate. In some embodiments, the silk fibroin fragments have a polydispersity between 1 and about 1.5. In some embodiments, the silk fibroin fragments have a polydispersity between about 1.5 and about 2.0. In some embodiments, the silk fibroin fragments have a polydispersity between about 1.5 and about 3.0. In some embodiments, the silk fibroin fragments have a polydispersity between about 2.0 and about 2.5. In some embodiments, the silk fibroin fragments have a polydispersity between about 2.5 and about 3.0. In some embodiments, the silk fibroin fragments are present in the composition at about 0.001 wt. % to about 10.0 wt. % relative to the total weight of the composition. In some embodiments, the composition further comprises about 0.001% (w / w) to about 10% (w / w) sericin relative to the total weight of the composition. In some embodiments, the composition further comprises about 0.001% (w / w) to about 10% (w / w) sericin relative to the silk fibroin fragments. In some embodiments, the silk fibroin fragments do not spontaneously or gradually gelate and do not visibly change in color or turbidity when in an aqueous solution for at least 10 days prior to formulation into the composition. In some embodiments, the silk fibroin fragments are present in the composition at about 0.01 wt. % to about 10.0 wt. % relative to the total weight of the composition. In some embodiments, the silk fibroin DB1 / 155183601.2 68fragments are present in the composition at about 0.01 wt. % to about 1.0 wt. % relative to the total weight of the composition. In some embodiments, the silk fibroin fragments are present in the composition at about 1.0 wt. % to about 2.0 wt. % relative to the total weight of the composition. In some embodiments, the silk fibroin fragments are present in the composition at about 2.0 wt. % to about 3.0 wt. % relative to the total weight of the composition. In some embodiments, the silk fibroin fragments are present in the composition at about 3.0 wt. % to about 4.0 wt. % relative to the total weight of the composition. In some embodiments, the silk fibroin fragments are present in the composition at about 4.0 wt. % to about 5.0 wt. % relative to the total weight of the composition. In some embodiments, the silk fibroin fragments are present in the composition at about 5.0 wt. % to about 6.0 wt. % relative to the total weight of the composition. In some embodiments, the composition is formulated as a topical composition. In some embodiments, the composition further comprises a pharmaceutically acceptable carrier. In some embodiments, the composition further comprises a dermatologically acceptable carrier. In some embodiments, the pharmaceutically acceptable carrier comprises one or more of a suspension, an emulsion, a powder, a solution, a dispersion, or an elixir. In some embodiments, the pharmaceutically acceptable carrier comprises or is formulated as one or more of a gel, a jelly, a cream, a lotion, a foam, a slurry, an ointment, an oil, a paste, a suppository, a spray, a semisolid composition, a solid composition, a stick, or a mousse. In some embodiments, the pharmaceutically acceptable carrier comprises one or more of sesame oil, corn oil, cottonseed oil, or peanut oil. In some embodiments, the pharmaceutically acceptable carrier comprises one or more of mannitol or dextrose. In some embodiments, the pharmaceutically acceptable carrier comprises free and / or uncrosslinked hyaluronic acid. In some embodiments, the pharmaceutically acceptable carrier comprises one or more of aliphatic oil, a fatty alcohol, a fatty acid, a glyceride, an acylglycerol, and a phospholipid. In some embodiments, the pharmaceutically acceptable carrier comprises one or more of a monoglyceride, a diglyceride, or a triglyceride. In some embodiments, the pharmaceutically acceptable carrier comprises an aqueous phase. In some embodiments, the pharmaceutically acceptable carrier comprises an oil-in-water emulsion or a water-in-oil emulsion. In some embodiments, the pharmaceutically acceptable carrier comprises one or more of DB1 / 155183601.2 69a hydrocarbon oil, a fatty acid, a fatty oil, a fatty acid ester, or a cationic quaternary ammonium salt. BRIEF DESCRIPTION OF THE DRAWINGS The presently disclosed embodiments will be further explained with reference to the attached drawings. The drawings shown are not necessarily to scale, with emphasis instead generally being placed upon illustrating the principles of the presently disclosed embodiments. Figure 1. Ion Exchange Fractionation Schemes for the isolation of the populations that constitute Low and Mid Skid silk / modified polypeptide compositions. Low and Mid Skid silk / modified polypeptide compositions contains silk / modified polypeptides that are negatively, positively charged, or neutral. Using Q anion exchange chromatography (A) these populations were isolated. Figure 2. Chromatogram of Low Skid silk / modified polypeptide composition loaded in a Q-Sepharose HP column (Cytiva). The flow through contains the silk / modified polypeptides that do not get captured in the column and are the depleted in negatively charged amino acids. After the column is loaded with Low or Mid Skid silk / modified polypeptide compositions and the flow through is collected, the column is washed until the UV-280 absorbance becomes less than 200 AU. The captured negatively charged silk / modified polypeptides are eluted with high salt concentration (1M NaCl) and constitute AS11 and AS22. The chromatography is performed in Tris- containing buffers but the flow through and the Q-elution were finally dialyzed in water. Figure 3. Analytical Size Exclusion Chromatography of Low, Mid Skid silk / modified silk compositions and their constituent AS compositions. Average molecular weight in kDa and polydispersity measurements are shown. Figures 4A- 4B. Analytical Size Exclusion Chromatography of the Low and Mid skid silk / modified peptide compositions and their components (see table 1 for more details). Fig.4A, Molecular weight of the various Activated Silk new compositions described in this study. Fig.4B, Polydispersity (PDI) of the various Activated Silk new compositions described in this study. AS24 reconstitutes the average molecular weight and polydispersity of the Low skid silk / modified peptide composition and it consists of 50% AS12 and 50% AS22 (see table 1 for details). AS6 reconstitutes the average molecular weight and polydispersity of the Mid skid DB1 / 155183601.2 70silk / modified peptide composition and it consists of 50% AS1 and 50% AS11 (see table 1 for details). Figure 5. Isoelectric Focusing Electrophoresis of Low Skid silk / modified polypeptide compositions. Lanes 2, 7; Low Skid silk different amounts loaded. Lanes 3, 5, 8, 10; AS12 silk, different preparations different amounts loaded. Lanes 4, 6, 9, 11; AS22 silk, different preparations different amounts loaded. Figures 6A- 6B. Self-assembly reactions of the of the Low and Mid skid silk / modified peptide compositions and their components (see table 1 for more details). Both graphs depict the kinetic parameters of gel formation during self- assembly of silk. On graph A calculation of the three self-assembly kinetic parameters is shown, t0.5, Amax and SARF. For more details look at the text. Figures 7A- 7C. Self-assembly kinetics of the Low and Mid skid silk / modified peptide compositions and their components (see table 1 for more details). Fig.7A, the Self-assembly Rate Factor shows how fast the self-assembly reaction proceeds once it is initiated and the self-assembly nuclei are organized. Fig. 7B, Maximum Gel Yield shows how dense the silk gel is after self-assembly is complete. Fig.7C, Time required for the self-assembly reaction to produce half of the maximum gel amount. Figure 8. the Low and Mid skid silk / modified peptide compositions and their components (see table 1 for more details). The Self Assembly Factor reflects the average propensity of silk to self-assemble and form gels. While the above-identified drawings set forth presently disclosed embodiments, other embodiments are also contemplated, as noted in the discussion. This disclosure presents illustrative embodiments by way of representation and not limitation. Numerous other modifications and embodiments can be devised by those skilled in the art which fall within the scope and spirit of the principles of the presently disclosed embodiments. Figure 9 is a graph of Weight average molecular weight (i.e., average molecular weight average or average MW) using Size exclusion chromatography with a refractive index detector (SEC-RI) plotted as a function of time for solubilized fibroin in 9.3 M LiBr at 100 °C - 103 °C (i.e., 3 degree Celsius temperature gradient between 100 °C and 103 °C). DB1 / 155183601.2 71Figure 10 is a graph of weight average molecular weight (i.e., average molecular weight average or average MW) using Size exclusion chromatography with a refractive index detector (SEC-RI) plotted as a function of time for solubilized fibroin in 9.3 M LiBr at 122 °C - 125 °C (i.e., 3 degree Celsius temperature gradient between 122 °C and 125 °C). Figure 11 is a graph illustrating percentage of amino acid modification in silk. Figures 12A-12C are graphs illustrating percentage of amino acid modifications in Low Skid Silk and Mid Skid silk. Fig.12A illustrates heavy chain modifications, Fig.12B illustrates light chain modifications, and Fig.12C illustrates fibrohexamerin modifications. N are Asparagines that become aspartic acid and Q are Glutamines that become deamidated. M corresponds to Methionies that become oxidized. The numbers after each amino acid show its position along the amino acid chain from the corresponding protein. Figures 13A- 13B are graphs illustrating percentage of amino acid modifications in Low Skid Silk and Mid Skid silk produced and lyophilized. Fig.13A illustrates heavy chain modifications and Fig.13B illustrates light chain modifications. N are Asparagines that become aspartic acid and Q are Glutamines that become deamidated. M corresponds to Methionies that become oxidized. The numbers after each amino acid show its position along the amino acid chain from the corresponding protein. Figures 14A- 14B are graphs illustrating percentage of amino acid modifications in Low Skid silk produced in Walpole and Medford using the Skid process with differing process parameters and variable levels. Fig.14A illustrates heavy chain modifications and Fig.14B illustrates light chain modifications. N are Asparagines that become aspartic acid and Q are Glutamines that become deamidated. M corresponds to Methionies that become oxidized. The numbers after each amino acid show its position along the amino acid chain from the corresponding protein. Figures 15A- 15D are graphs illustrating percentage of amino acid modifications in Low and Mid silk produced in Skid and Benchtop processes. N are Asparagines that become aspartic acid and Q are Glutamines that become deamidated. M corresponds to Methionies that become oxidized. The numbers after each amino acid show its position along the amino acid chain from the corresponding protein. DB1 / 155183601.2 72Figure 16 is an explanation of the method used to calculate percentage ratios of modified amino acids at specific locations along the sequence of each peptide. Figure 17 illustrates an Anion exchange chromatography and size exclusion chromatography scheme of the isolation of Low Skid silk / modified peptide compositions. Low Skid silk / modified polypeptide compositions is composed of a variety of peptide populations, in a wide range of sizes and charge. Using Q- Sepharose anion exchange chromatography as a first step, and HiLoad Superdex 200 size exclusion chromatography as a second purification step, distinct populations of Low Skid silk / modified polypeptide compositions were separated. The Q-Sepharose eluate was loaded onto HiLoad Superdex 200 size exclusion chromatography, which resulted in negatively charged silk compositions / modified peptides fractionated by size. Figures 18A and 18B are chromatograms of the anion exchange chromatography and the following size exclusion chromatography of the eluate (Q- eluate) of Low Skid silk / modified polypeptide compositions. Fig. 18A: Anionexchange chromatography was performed with a Q-Sepharose column (Cytiva). Low Skid silk / modified peptide compositions were separated to uncharged peptide population (flowthrough – light blue background) and eluted negatively charged silk compositions (eluate – light pink background) by anion exchange chromatography. Light yellow background indicates column wash with 50 mM Tris pH=8.0 before eluting the charged peptide population. Fig.18B: The negatively charged eluate was loaded onto the Superdex 200 column and was flowed through the column with 50 mM Tris, 200 mM CaCl2, pH=8.0. When the UV-280 absorbance started to increase fractions were collected to separate the Low Skid silk / modified peptide compositions by size. The relative elution volume of silk compositions AS77 and AS81 are indicated on the chromatogram. Figures 19A and 19B illustrates the Analytical Size Exclusion Chromatography of Low Skid silk / modified silk compositions and their constituent AS compositions. Fig. 19A. Average molecular weight in kDa of Low Skid silk (LS)and AS77-AS81 are shown. Fig. 19B. Polydispersity (PDI) measurements are shown.The numerical data is presented in Table 7. DB1 / 155183601.2 73Figure 20 is a SDS polyacrylamide gel electrophoresis of Low Skid silk / modified polypeptide compositions. Lanes are indicated by fraction number, at the order of elution from the Superdex 200 column, and their respective silk composition: fraction 6 is AS77, fraction 7 is AS78, fraction 8 is AS79, fraction 9 is AS80, and fraction 10 is AS81. Figures 21A and 21B are graphs illustrating self-assembly reactions of the of the Low Skid silk / modified peptide compositions. Mid Skid Silk reaction was used as apositive control. Fig. 21A. Illustrate kinetic parameters of gel formation during self-assembly of silk. Self-Assembly parameters of Mid Skid silk: Amax is 0.6780 (Abs), SARF is 8.676, T0.5 is 3.668 h, and the FSAF is 3.08 (Abs / min). Fig. 21B. Is asnapshot of a later time point of the same self-assembly assay, 12 days after setting the assay. None of the tested fraction has self-assembled over time. Figures 22A and 22B illustrate the characterization of Low Skid silk compositions by Dynamic Light Scattering. Low skid silk / modified peptide compositions were diluted to a concentration of 1 mg / mL, filtered, and analyzed by the Zetasizer Pro to estimate the diameter particle size of each silk composition. Fig. 22A. Illustrates intensity diameter particle size distribution measured for silk compositions AS77, AS78, AS79, AS80, and AS81. Fig. 22B. Illustrate correlogramfunctions of silk compositions AS77, AS78, AS79, AS80, AS81. Figure 23 illustrates size exclusion chromatography scheme of the isolation of Low Skid silk / modified peptide compositions. Low Skid silk / modified polypeptide compositions is composed of a variety of peptide populations, in a wide range of sizes, using HiLoad Superdex 200 size exclusion chromatography, distinct populations of Low Skid silk / modified polypeptide compositions were separated. Figure 24 is a chromatogram of Low Skid silk / modified polypeptide compositions loaded onto a Superdex 200 gel filtration column. Low Skid silk / modified peptide compositions were loaded onto the Superdex 200 column and were flowed through the column with 50 mM Tris, 200 mM CaCl2, pH=8.0. When the UV-280 absorbance started to increase fractions were collected to separate the Low Skid silk / modified peptide compositions by size. The relative elution volume of silk compositions AS82, AS86, and AS87 are indicated on the chromatogram. DB1 / 155183601.2 74Figures 25A and 25B illustrate Analytical Size Exclusion Chromatography of Low Skid silk / modified silk compositions and their constituent AS compositions. Fig. 25A. Illustrates average molecular weight in kDa of Low Skid silk (LS) and AS82- AS89 are shown. Fig. 25B. Polydispersity (PDI) measurements are shown. Thenumerical data is presented in Table 9. Figure 26 is an SDS polyacrylamide gel electrophoresis of Low Skid silk / modified polypeptide compositions. Lanes are indicated by fraction number, at the order of elution from the Superdex 200 column, and their respective silk composition: fraction 6 is AS82, fraction 7 is AS83, fraction 8 is AS84, fraction 9 is AS85, and fraction 10 is AS86. Figures 27A and 27B are graphs illustrating self-assembly reactions of the of the Low Skid silk / modified peptide compositions. Mid Skid Silk reaction was used as apositive control. Fig. 27A. Illustrates kinetic parameters of gel formation duringself-assembly of silk. Self-Assembly parameters of Mid Skid silk: Amax is 0.6978 (Abs), SARF is 8.591, T0.5 is 3.361 h, and the FSAF is 3.46 (Abs / min). Fig. 27B. Is asnapshot of a later time point of the same self-assembly assay, 18 days after setting the assay. AS87, AS88, and AS89 demonstrate gel formation at this time point, that was already observed five days post assay (LS, Low Skid silk; MS, Mid Skid silk). Figures 28A- 28C are graphs showing characterization of Low Skid silk compositions by Dynamic Light Scattering. Low skid silk / modified peptide compositions were diluted to a concentration of 1 mg / mL, filtered, and analyzed by the Zetasizer Pro to estimate particle size of each silk composition. Fig. 28A. Showsintensity particle size distribution measured for silk compositions AS82, AS83, AS84, AS85, AS86, AS87, AS88, and AS89. Fig. 28B. Shows intensity particle sizedistribution measured for silk compositions AS82, Low Skid silk / modified peptide compositions (LS), and Mid Skid silk / modified peptide compositions (MS). Fig. 28C.Shows correlogram functions of silk compositions AS82, AS83, AS84, AS85, AS86, AS87, AS88, AS89, Low Skid silk / modified peptide compositions (LS), Mid Skid silk / modified peptide compositions (MS). Figure 29 illustrates anion exchange chromatography (Q), hydrophobic interaction chromatography (HIC), and size exclusion chromatography (SEC) scheme of the isolation of Low Skid silk / modified peptide compositions. Low Skid DB1 / 155183601.2 75silk / modified polypeptide compositions is composed of a variety of peptide populations, in a wide range of sizes and charge. Using Q-Sepharose anion exchange chromatography as a first step, Butyl ImpRes Hydrophobic interactions resin as a second step, and HiLoad Superdex 200 size exclusion chromatography as a third purification step, distinct populations of Low Skid silk / modified polypeptide compositions were isolated. The Q-Sepharose eluate was loaded onto a Butyl ImpRes (HIC) column, and the HIC-eluate was loaded onto a HiLoad Superdex 200 size exclusion chromatography, which resulted in fractionation of negatively charged silk compositions / modified peptides with hydrophobicity characteristics fractionated by size. The Q-Sepharose eluate contained negatively charged peptides in all sizes. Resolving these peptides by Butyl ImpRes column resulted in elution of high- molecular-weight, negatively charged, somewhat hydrophobic silk compositions / modified peptides. The smaller negatively charged peptides were washed as flowthrough and did not bind the Butyl ImpRes column. The Q- HIC(elution) was loaded into Superdex 200 and was separated by size. Figures 30A- 30E are chromatograms of anion exchange chromatography, hydrophobic interactions chromatography, and the following size exclusion chromatography of Low Skid silk / modified polypeptide compositions. Fig.30A. illustrates anion exchange chromatography was performed with a Q-Sepharose column. Low Skid silk / modified peptide compositions were separated to uncharged peptide population (flowthrough – light blue background) and eluted negatively charged silk compositions (eluate – light pink background) by anion exchange chromatography. Light yellow background indicates column wash with 50 mM Tris pH=8.0 before eluting the charged peptide population. Fig.30B. illustrates the negatively charged eluate (Q-elution) was loaded onto a Butyl ImpRes column, in the presence of 300 mM ammonium sulfate [(NH4)2SO4], to expose hydrophobic domains of the silk peptides, which allows binding to the column. The highly charged peptide population did not bind the column (flowthrough), highlighted in light blue. The column was washed until OD280 was reduced to ~100 units (light yellow). Then, the bound silk peptides (Q-HIC(elution)) were eluted by using 50 mM Tris, pH=8.0 without ammonium sulfate (light pink). Fig.30C. illustrates the Q-HIC(elution) was further fractionated by size exclusion chromatography (SEC), using the gel filtration column Superdex 200. The Q-HIC(elution) fraction was flowed through the column DB1 / 155183601.2 76with 50 mM Tris, 200 mM CaCl2, pH=8.0. When the UV-280 absorbance started to increase fractions were collected to separate the Low Skid silk / modified peptide compositions by size. The relative elution volume of silk compositions AS90 and AS94 are indicated on the chromatogram. Fig.30D. the Q-HIC(flowthrough) fraction was further fractionated by SEC, using the Superdex 200 column, at the same procedure as in (VC). The relative elution volume of silk compositions AS95 and AS100 are indicated on the chromatogram. Fig.30E. illustrates the superimposition of chromatograms (VC) and (VD). the Q-HIC(elution) fraction has a higher-molecular- weight range compared to the Q-HIC(flowthrough) fractions, which elutes later in SEC, and has lower-molecular-weight range. Figures 31A- 31B are graphs showing analytical Size Exclusion Chromatography of Low Skid silk / modified silk compositions and their constituent AS compositions. Fig.31A. Average molecular weight in kDa of Low Skid silk (LS) and AS90-AS100 are shown. Fig.31B. Polydispersity (PDI) measurements are shown. The numerical data is presented in Table 11. Figures 32A- 32B are SDS polyacrylamide gel electrophoresis of Low Skid silk / modified polypeptide compositions. Fig.32A. Q-HIC(elution) SEC fractions. Fig.32B. Q-HIC(flowthrough) SEC fractions. Lanes are indicated by fraction number, at the order of elution from the Superdex 200 column, and their respective silk composition: in Fig.32A, fraction 6 is AS90, fraction 7 is AS91, fraction 8 is AS92, fraction 9 is AS93, and fraction 10 is AS94. In Fig.32B, fraction 8 is AS95, fraction 9 is AS96, fraction 10 is AS97, fraction 11 is AS98, fraction 12 is AS99, and fraction 13 is AS100. Figure 33 illustrates self-assembly reactions of the of the Low Skid silk / modified peptide compositions. Mid Skid Silk reaction was used as a positive control. kinetic parameters of gel formation during self-assembly of silk. Q- HIC(elution) is the elution fraction that was eluted from the Butyl ImpRes column, prior to SEC purification; LS, Low Skid silk; MS, Mid Skid silk. Self-Assembly parameters of Mid Skid silk: Amax is 0.6974 (Abs), SARF is 8.661, T0.5 is 3.834 h, and the FSAF is 3.03 (Abs / min). Figures 34A- 34F illustrate the characterization of Low Skid silk compositions by Dynamic Light Scattering. Low and Mid skid silk / modified peptide DB1 / 155183601.2 77compositions were diluted to a concentration of 1 mg / mL, filtered, and analyzed by the Zetasizer Pro to estimate the diameter particle size of each silk composition. Fig. 34A. Intensity diameter particle size distribution measured for silk compositions AS90, Q-HIC(elution) fraction (prior to fractionation by SEC), Low Skid silk (LS), and Mid Skid silk (MS). Fig.34B. Correlogram functions of silk compositions presented in (34A). Fig.34C. Intensity diameter particle size distribution measured for silk compositions AS90-AS94, derived from Q-HIC(elution)-SEC fractionation process. Fig.34D. Correlogram functions of silk compositions presented in (34C). Fig.34E. Intensity diameter particle size distribution measured for silk compositions AS95-AS100, derived from Q-HIC(flowthrough)-SEC fractionation process. Fig. 34F. Correlogram functions of silk compositions presented in (34E). Figure 35. illustrates size exclusion chromatography scheme of the isolation of Mid Skid silk / modified peptide compositions. Mid Skid silk / modified polypeptide compositions is composed of a variety of peptide populations, in a wide range of sizes, using HiLoad Superdex 200 size exclusion chromatography, distinct populations of Mid Skid silk / modified polypeptide compositions were able to be separated. Figure 36. Is a chromatogram of Mid Skid silk / modified polypeptide compositions loaded onto a Superdex 200 gel filtration column. Mid Skid silk / modified peptide compositions were loaded onto the Superdex 200 column and were flowed through the column with 50 mM Tris, 200 mM CaCl2, pH=8.0. When the UV-280 absorbance started to increase fractions were collected to separate the Mid Skid silk / modified peptide compositions by size. The relative elution volume of silk compositions AS107 and AS111 are indicated on the chromatogram. Figures 37A- 37B. Illustrates analytical Size Exclusion Chromatography of Mid Skid silk / modified silk compositions and their constituent AS compositions. Fig. 37A. Average molecular weight in kDa of Mid Skid silk (MS) and AS106-AS111 are shown. Fig.37B. Polydispersity (PDI) measurements are shown. The numerical data is presented in Table 14. Figure 38. Is a SDS polyacrylamide gel electrophoresis of Mid Skid silk / modified polypeptide compositions. Lanes are indicated by fraction number, at the order of elution from the Superdex 200 column, and their respective silk DB1 / 155183601.2 78composition: fraction 6 is AS107, fraction 7 is AS108, fraction 8 is AS109, fraction 9 is AS110, and fraction 10 is AS111. Figure 39. Illustrates self-assembly reactions of the of the Mid Skid silk / modified peptide compositions. Kinetic parameters of gel formation during self- assembly of silk. Dashed red lines show how the self-assembly parameters Amax, SARF, and T0.5 were calculated for unfractionated Mid Skid silk (MS). These numerical calculated parameters of silk compositions AS106-AS111 can be found in Table 16. Low Skid silk (LS) was used as a negative control. LS, Low Skid silk; MS, Mid Skid silk. Figures 40A-40B. Illustrates characterization of Mid Skid silk compositions by Dynamic Light Scattering. Mid skid silk / modified peptide compositions were diluted to a concentration of 1 mg / mL, filtered, and analyzed by the Zetasizer Pro to estimate particle size of each silk composition. Fig.40A. Intensity particle size distribution measured for silk compositions AS106, AS107, AS108, AS109, AS110, AS111, and Mid Skid (MS). Fig.50B. Correlation functions of silk compositions presented in (40A). Figure 41. Illustrates anion exchange chromatography and size exclusion chromatography scheme of the isolation of Mid Skid silk / modified peptide compositions. Mid Skid silk / modified polypeptide compositions is composed of a variety of peptide populations, in a wide range of sizes and charge. Using Q- Sepharose anion exchange chromatography as a first step, and HiLoad Superdex 200 size exclusion chromatography as a second purification step, distinct populations of Mid Skid silk / modified polypeptide compositions were separated. The Q-Sepharose eluate was loaded onto HiLoad Superdex 200 size exclusion chromatography, which resulted in negatively charged silk compositions / modified peptides fractionated by size. Figures 42A-42B. Are chromatograms of the anion exchange chromatography and the following size exclusion chromatography of the eluate (Q-eluate) of Mid Skid silk / modified polypeptide compositions. Fig.42A. Anion exchange chromatography was performed with a Q-Sepharose column (Cytiva). Mid Skid silk / modified peptide compositions were separated to uncharged peptide population (flowthrough – light blue background) and eluted negatively charged silk compositions (eluate – light pink DB1 / 155183601.2 79background) by anion exchange chromatography. Light yellow background indicates column wash with 50 mM Tris pH=8.0 before eluting the charged peptide population. Fig.42B. The negatively charged eluate (Q-elution) was loaded onto the Superdex 200 column and was flowed through the column with 50 mM Tris, 200 mM CaCl2, pH=8.0. When the UV-280 absorbance started to increase fractions were collected to separate the Mid Skid silk / modified peptide compositions by size. The relative elution volume of silk compositions AS101 and AS105 are indicated on the chromatogram. Figures 43A-43B. Illustrate analytical Size Exclusion Chromatography of Mid Skid silk / modified silk compositions and their constituent AS compositions. Fig. 43A. Average molecular weight in kDa of Mid Skid silk (MS) and AS101-AS105 are shown. Fig.43B. Polydispersity (PDI) measurements are shown. The numerical data is presented in Table 16. Figure 44A. Is a SDS polyacrylamide gel electrophoresis of Mid Skid silk / modified polypeptide compositions. Lanes are indicated by fraction number, at the order of elution from the Superdex 200 column, and their respective silk composition: fraction 6 is AS101, fraction 7 is AS102, fraction 8 is AS103, fraction 9 is AS104, and fraction 10 is AS105. Figure 44B illustrates self-assembly reactions of the of the Mid Skid silk / modified peptide compositions. Low Skid Silk reaction was used as a negative control. Kinetic parameters of gel formation during self-assembly of silk are shown. Red dotted lines are shown to clarify the calculations of Amax, SARF (Self-Assembly Rate Factor), and T0.5 parameters in Table 17. Figures 45A-45C. Are graphs illustrating characterization of Mid Skid silk compositions by Dynamic Light Scattering. Mid skid silk / modified peptide compositions were diluted to a concentration of 1 mg / mL, filtered, and analyzed by the Zetasizer Pro (Malvern) to estimate the diameter particle size of each silk composition. Fig.45A. Intensity diameter particle size distribution by intensity measured for silk compositions AS101, AS102, AS103, AS104, and AS105. Fig. 45B. Intensity diameter particle size distribution by intensity measured for silk compositions AS101, AS105, and Mid Skid silk (MS), to emphasize the size difference between AS101 and AS105. Fig.45C. Correlogram functions of silk compositions AS101, AS102, AS103, AS104, AS105, and Mid Skid silk (MS). Figure 46. is an illustration of the values for three molar mass moments (Mn, Mw, and Mz) as it relates to molar mass and the number of molecules at each molar DB1 / 155183601.2 80mass. This example is applicable to a polydisperse sample; for a monodisperse sample, Mn = Mw = Mz. Figures 47A- 47B are analytical SEC-MALS of Low, Mid and High Molecular Weight Silk. Fig.47A. Weight Average Molecular Weight in kDa of Low, Mid, and High Molecular Weight Silk. Fig.47B. Polydispersity Index (PDI) measurements of Low, Mid, and High Molecular Weight Silk are shown. Figures 48A- 48B. Are analytical SEC-MALS of Low, Mid and High Molecular Weight Silk organized by Silk Type produced by different process parameters and variable levels. Individual data points are shown and the mean is represented by the heigh of the box. The bars encompass one standard deviation. Fig. 48A. Weight-Average Molecular Weight Ranges for Low, Mid, and High Molecular Weight Silk. Fig.48B. PDI Ranges for Low, Mid, and High Molecular Weight Silk. Figures 49A- 49B are analytical SEC-MALS of Low Skid silk / modified silk compositions and the constituent AS compositions as separated by Q-SEC (Q-eluent). Fig.49A. Average molecular weight in kDa of Low Skid silk (LS) and AS77-AS81 are shown. Fig.49B. Polydispersity (PDI) measurements are shown. The numerical data is presented in Table 24. Figures 50A- 50B are analytical SEC-MALS of Low Skid silk / modified silk compositions and the constituent AS compositions as separated by SEC. Fig.50A. Average molecular weight in kDa of Low Skid silk (LS) and AS82-AS89 are shown. Fig.50B. Polydispersity (PDI) measurements are shown. The numerical data is presented in Table 25. Figures 51A- 51B are analytical SEC-MALS of Low Skid silk / modified silk compositions and the constituent AS compositions as separated by Q-HIC-SEC (Q- HIC-Eluent). Fig.51A. Average molecular weight in kDa of Low Skid silk (LS) and AS90-AS94 are shown. Fig.51B. Polydispersity (PDI) measurements are shown. The numerical data is presented in Table 26. Figures 52A- 52B are analytical SEC-MALS of Low Skid silk / modified silk compositions and the constituent AS compositions as separated by Q-HIC-SEC (Q- HIC-Flowthrough). Fig.52A. Average molecular weight in kDa of Low Skid silk (LS) and AS95-AS100 are shown. Fig.52B. Polydispersity (PDI) measurements are shown. The numerical data is presented in Table 26. Figures 53A- 53B are analytical SEC-MALS of Mid Skid silk / modified silk compositions and the constituent AS compositions as separated by Q--SEC (Q-flow DB1 / 155183601.2 81through). Fig.53A. Average molecular weight in kDa of Mid Skid silk (MS) and AS101-AS105 are shown. Fig.53B. Polydispersity (PDI) measurements are shown. The numerical data is presented in Table 27. Figures 54A- 54B are analytical SEC-MALS of Mid Skid silk / modified silk compositions and the constituent AS compositions as separated by SEC. Fig.54A. Average molecular weight in kDa of Mid Skid silk (MS) and AS106-AS111 are shown. Fig.54B. Polydispersity (PDI) measurements are shown. The numerical data is presented in Table 28. Figure 55 is a chart illustrating mean TEWL values for barrier redux emulsion. Figure 56 is a chart illustrating the effect of the barrier redux emulsion on wrinkles and photoaging. Figure 57 is a chart illustrating the effect of the barrier redux emulsion on hyperpigmentation. Figure 58 is a chart illustrating the effect of the barrier redux emulsion on wrinkles. Figure 59 is a chart illustrating the effect of the barrier redux emulsion on crow’s feet wrinkles. Figure 60 is a chart illustrating the effect of the barrier redux emulsion on crow’s feet lines. Figure 61 is a chart illustrating effect of the barrier redux emulsion on marionette area wrinkles. Figure 62 is a chart illustrating the effect of the barrier redux emulsion on skin roughness. Figure 63 is a chart illustrating the effect of the barrier redux emulsion on global wrinkles. Figure 64 is a chart illustrating the effect of the barrier redux emulsion on global fine lines. Figure 65 is a chart illustrating the effect of the barrier redux emulsion on the appearance of medium, deep lines and wrinkles. Figure 66 is a chart illustrating the effect of the barrier redux emulsion on skin tone evenness. Figure 67 is a chart illustrating the effect of the barrier redux emulsion on skin texture. DB1 / 155183601.2 82Figure 68 is a chart illustrating the effect of the barrier redux emulsion on skin scaling. Figures 69A – 69C show the sequence listing for fibroin heavy chain. Figure 70 shows the sequence listing for fibroin light chain. Figure 71 shows the sequence listing for fibrohexamerin. Figure 72 is a graph illustrating absorbency of different fabrics using laundry pods with activated silk. Figure 73 is a flow chart showing various embodiments for producing silk fibroin protein fragments (SPFs) of the present disclosure. Figure 74 is a flow chart showing various parameters that can be modified during the process of producing a silk protein fragment solution of the present disclosure during the extraction and the dissolution steps. Figure 75 is a chart showing absorbency of Activated SilkTM with Capryl / Caprylyl glucoside coating on broad range of interlock nylon fabrics; unfinished nylon interlock fabrics are not absorbing water having poor absorbency; after Activated SilkTM with Capryl / Caprylyl glucoside coating, the absorbency of all the nylon interlock fabrics are significantly increased. Figure 76 is a chart showing absorbency of Activated SilkTM with Capryl / Caprylyl glucoside coating on various nylon fabrics other than interlock structure; unfinished nylon fabrics are not absorbing water or having poor absorbency; after Activated SilkTM with Capryl / Caprylyl glucoside coating, the absorbency of all the nylon fabrics are significantly increased. Figure 77 is a chart showing the moisture absorbency curve with no washing generated by changing the concentration of polyoxyethylene (29) castor oil in the mixture of emulsifiers (thereby changing the HLB) before adding to the coating solution; in all samples the silk concentration in the coating solution 1 g / L. Figure 78 is a chart showing the hand feel ranking curve with no washing generated by changing the concentration of polyoxyethylene (29) castor oil in the mixture of emulsifiers (thereby changing the HLB) before adding to the coating solution; in all samples the silk concentration in the coating solution 1 g / L. Figures 79A-79D are charts showing the moisture management data for no washes (Fig.79A), 5 washes (Fig.79B), 10 washes (Fig.79C), and 25 washes (Fig. 79D) generated by changing the concentration of the emulsion mixture (Polyoxyethylene (20) sorbitan monooleate, Polyoxyethylene (20) sorbitan trioleate, DB1 / 155183601.2 83Polyoxyethylene (29) castor oil, and water in a 2:4:8:10 ratio) in the final coating solution; in all samples the silk concentration in the coating solution 1 g / L. Figures 80A-80D are charts showing the hand feel ranking results from no washes (Fig.80A), 5 washes (Fig.80B), 10 washes (Fig.80C), and 25 washes (Fig. 80D) generated by changing the concentration of the emulsion mixture (Polyoxyethylene (20) sorbitan monooleate, Polyoxyethylene (20) sorbitan trioleate, Polyoxyethylene (29) castor oil, and water in a 2:4:8:10 ratio) in the final coating solution; 1 is the best score and 8 is the worst hand ranking score; in all samples the silk concentration in the solution 1 g / L. Figures 81A-81D are charts showing the hand feel ranking results from no washes (Fig.81A), 5 washes (Fig.81B), 10 washes (Fig.81C), and 25 washes (Fig. 81D) generated by changing the concentration of mid molecular weight silk in the final coating solution; 1 is the best ranking and 8 is the worst ranking Figures 82A-82D are charts showing the moisture management results from no washes (Fig.82A), 5 washes (Fig.82B), 10 washes (Fig.82C), and 25 washes (Fig.82D) generated by changing the concentration of mid molecular weight silk in the final coating solution. Figures 83A-83D are graphs showing UV / Vis quantification experiments of fabrics coated with low molecular weight activated silk and polyoxyethylene (20) monooleate solution. Fig.83A: A graph showing the percent of silk lost after five washes with respect to fiber surface area. Fig.83B: A graph showing the mass quantified of silk on the fabric after coating with respect to fiber surface area. Fig. 83C: A graph showing the percent of silk lost after five washes with respect to fabric type. Fig.83D: A graph showing the mass of silk quantified on each fabric before and after five washes depending on fabric type. Figure 84 is a chart showing UV / Vis quantification experiments of fabrics coated with low molecular weight activated silk and polyoxyethylene (20) monooleate solution. A graph showing the mass quantified of silk on the fabric after coating with respect to fabric mass in grams per square meter (GSM). This mass is dependent on knit type, fiber content, and filament denier. Figures 85A- 85C include a series of charts showing potentiometric titration curves with the charge density measured at a pH of 5 for the unfinished heavy weight DB1 / 155183601.2 84double knit nylon fabric (Fig.85A), activated silk finished heavy weight double knit nylon fabric (Fig.85B), and Archroma RPU wetting agent finished heavy weight double knit nylon fabric (Fig.85C). Each fabric has a titration curve obtained at no washes (Figs.85A-85C, left panels) and at five washes (Figs.85A-85C, right panels). The change in charge density at pH 5 after washing is denoted as ΔC. Figure 86 illustrates experimental setup for Example 18. Samples of fabric were prepared and washed for five washes in a front-loading washer and surface charging was measured as the difference in charge before and after washing. Figure 87 is a diagram showing the location and description of the zeta potential of a surface. Figure 88 is a graph showing the change in zeta potential before and after five washes for five different fabric types and three different finishing types. Figure 89 is a diagram showing the effective surface charge has on surrounding pH due to proton and hydroxide surface adsorption. Figure 90 is a graph showing the change in charge density at pH 5 before and after five washes for five different fabric types. The control section denotes the unfinished fabric and the AS 320 section denotes the silk finished fabrics. Figure 91 is a graph illustrating the UV / Vis spectrum of synthesized Silver Silk Nanoparticles tested in Example 19. Figure 92 is a graph showing absorbency of the unfinished, Nigh Blue DC98- IA pretreated with fixing agent only, and AS-320 coated Night Blue DC98-IA, pretreated with cationic fixing agent from T=0 to T=10. Figure 93 is a graph showing absorbency of the unfinished, Black DC95-FA pretreated with fixing agent only, and AS-320 coated Black DC95-FA, pretreated with cationic fixing agent from T=0 to T=10. Figure 94 is a graph showing absorbency of unfinished at T=0, and AS-320 coated (15g / L, with and without HT-232H fixing agent pretreatment) 17120513 black from T=0 to T=10. Figure 95 is a graph showing absorbency of unfinished at T=0, and AS-320 coated (15g / L, with and without HT-232H fixing agent pretreatment) 17120511 blue from T=0 to T=10. DB1 / 155183601.2 85Figure 96 is a graph illustrating the improvement in Investigator Global Assessment (IGA) after using the Body Wash. Figure 97 is a graph illustrating the improvement in redness after using the Body Wash. Figure 98 is a graph illustrating the improvement in eczema count after using the Body Wash. Figure 99 is a graph illustrating the improvement in individual corneometer after using the Body Wash. Figure 100 is a graph illustrating the improvement in individual TEWL after using the Body Wash. Figure 101 is a graph illustrating responses of the Self-perception Questionnaires after using the Body Wash. Figure 102 is a graph illustrating responses of the Self-perception Questionnaires after using the Body Wash. Figure 103 illustrates three chromatography principles of silk fractionalization. Figure 104 illustrates the size exclusion chromatography of silk fractionalization. Figure 105 illustrates the anion exchange chromatography followed by size exclusion chromatography of silk fractionalization. Figure 106 illustrates the anion exchange chromatography followed by hydrophobic interactions chromatography and size exclusion chromatography. Figure 107 is a chart summarizing the three pipelines of silk fractionalization. Figure 108 is the characterizations methods of silk fractionalization. Figure 109 illustrates size exclusion chromatography of Low Skid silk. Figure 110 illustrates an example of silk composition characterization: Size exclusion chromatography of Low Skid silk. Figure 111 illustrates an example of silk composition characterization: Size exclusion chromatography of Low Skid silk Dynamic light scattering. Figure 112 is a chart including Z-averages of Low Skid silk / modified polypeptide composition. Figure 113 illustrates molecular weight and Polydispersity determination by HPLC. DB1 / 155183601.2 86Figure 114 illustrates Silk fractionation Size exclusion chromatography of Low Skid silk. Lower-molecular- weight fractions self-assemble slowly over time. Figure 115 is a chart including assays for characterizing silk fractions. Figure 116 is a comparison of Molecular weight and Polydispersity determination by two methods. Figure 117 shows graphs presenting the data of Tables 70 and 71. Data shown with standard deviation. “Nanoclay” refers to Elementis Bentone Hydroclay. Figure 118 is as diagram of the typical bentonite clay structure. Figure 119 shows SEM images of cross-sectional area of film cast with Elementis Bentone Hydroclay 2001. Highly-ordered stacking of clay layers is visible. Figure 120 is an SEM image of cross-sectional area of film cast with pure RSF. Figure 121 is an SEM image of cross-sectional area of 1:1 RSF / 2001 film cast under neutral (pH ~7.0) conditions. The layered structure of the clay is retained. Figure 122 is an SEM image of cross-sectional area of 1:1 RSF / 2001 film cast under acidic (pH ~3.5) conditions. Note the ribbon-like structure. Figure 123 illustrates the increased diffusive pathway created by the RSF / nanoclay composite. Figure 124 is an FTIR scan of the amide I region of RSF / 2001 films cast under neutral conditions. The concentration of nanoclay is varied from 0% (red) to 70% (yellow). As nanoclay content increases, the amide I peak shifts left, away from the beta sheet region. Figures 125A- 125C are graphs amino acid abundance of low skid silk and derivatives. Figures 126A- 126C are graphs amino acid abundance of mid skid silk and derivatives. Figure 127 is a graph illustrating molecular weights of the silk compositions disclosed in this application as determined with HPLC. Figures 128A- 128B are graphs showing DSF traces of low skid silk (Fig. 128A) and mid skid (Fig.128B)silk with and without 20% IPA. The derivative of the fluorescence was normalized to the highest intensity and then plotted over the temperature range. The graphs represent averages of three technical repeats. Figure 129A shows graphs of DSF traces of low skid mid skid silk without 20% IPA. The derivative of the fluorescence was normalized to the highest intensity DB1 / 155183601.2 87Figure 129B shows graphs of DSF traces of low skid and mid skid silk with 20% IPA. The derivative of the fluorescence was normalized to the highest intensity Figure 130 is a model that illustrates the structural dynamic transitions observed in the DSF experiments. Figure 131 is a schematic overview of crosslinked Low Skid silk / unnatural peptide generation and isolation of different peptide compositions. Low Skid silk / unnatural polypeptide compositions are crosslinked with EDC, generating new peptide compositions. Figure 132 is a size exclusion chromatography and multi-angle light scattering (SEC-MALS) of crosslinked Low Skid silk / unnatural silk compositions and their constituent AS compositions. Average molecular weight in kDa of crosslinked Low Skid silk (LS+EDC, AS118), Low Skid silk (LS) and AS112-AS117 are shown. Figure 133 is a schematic overview of crosslinked Low Skid silk / unnatural peptide generation and isolation of different peptide compositions. By using HiLoad Superdex 200 size exclusion chromatography, distinct populations of crosslinked Low Skid silk / unnatural polypeptide compositions were separated, based on their molecular size. Figure 134A is a chromatogram of crosslinked Low Skid silk / unnatural polypeptide compositions loaded onto a Superdex 200 gel filtration column (Cytiva). The relative elution volume of silk compositions AS112 and AS116 are indicated on the chromatogram. Figure 134B is a SDS polyacrylamide gel electrophoresis of crosslinked Low Skid silk / unnatural polypeptide compositions. SDS-PAGE analysis of isolated crosslinked Low Skid silk / unnatural polypeptide compositions AS112-AS117. Lanes are indicated by fraction number, at the order of elution from the Superdex 200 column, and their respective silk composition: fraction 6 is AS112, fraction 7 is AS113, fraction 8 is AS114, fraction 9 is AS115, fraction 10 is AS116, and fraction 11 is AS117. Figures 135A – 135B are size exclusion chromatography and multi-angle light scattering (SEC-MALS) of crosslinked Low Skid silk / unnatural silk compositions and their constituent AS compositions. Fig.135A is Average molecular weight in kDa of crosslinked Low Skid silk (LS+EDC, AS118), Low Skid silk (LS) and AS112-AS117 are shown. Fig.135B Polydispersity (PDI) measurements are shown. DB1 / 155183601.2 88Figures 136A – 135D are graphs illustrating the Characterization of crosslinked Low Skid silk / unnatural peptide compositions by Dynamic Light Scattering. Silk compositions were diluted to a concentration of 1 mg / mL, filtered, and analyzed by the Zetasizer Pro (Malvern) to estimate particle size distribution of each silk composition. Fig.136A. Intensity particle size distribution measured for silk compositions AS112-AS117. Fig.136B. Intensity particle size distribution measured for crosslinked (LS+EDC) and non-crosslinked Low Skid silk / unnatural peptide compositions (LS), compared to AS112. Fig.136C. Correlogram functions of silk compositions AS112-AS117. Fig.136D. Correlogram functions of crosslinked (LS+EDC) and non-crosslinked Low Skid silk / unnatural peptide compositions (LS), and AS112. Figures 137A- 137B Self-assembly reactions of the of the crosslinked and non-crosslinked Low Skid silk / unnatural peptide compositions. Mid Skid Silk reaction was used as a positive control. Fig.137A. kinetic parameters of gel formation during self-assembly of silk. Self-Assembly parameters of Mid Skid silk: Amax is 0.6858 (Abs), SARF is 8.226, T0.5 is 6.574 h, and the FSAF is 1.739 (Abs / min). Fig. 137B. A snapshot of a later time point of the same self-assembly assay, 72 h after setting the assay. Crosslinked Low Skid silk / unnatural peptide compositions (LS+EDC) demonstrate gel formation at this time point (LS, Low Skid silk; MS, Mid Skid silk). DETAILED DESCRIPTION In some embodiments, compositions of the present disclosure include peptides compositions selected from compositions #1001 to #2450, having weight average molecular weights selected from about 1 kDa to about 145 kDa, and a polydispersity selected from between 1 and about 5 (including, without limitation, a polydispersity of 1), between 1 and about 1.5 (including, without limitation, a polydispersity of 1), between about 1.5 and about 2, between about 1.5 and about 3, between about 2 and about 2.5, between about 2.5 and about 3, between about 3 and about 3.5, between about 3.5 and about 4, between about 4 and about 4.5, and between about 4.5 and about 5. In some embodiments, these peptide compositions include amino acid modifications, substitutions, and / or replacements as defined herein. Methods of making silk fibroin or silk fibroin protein fragments and their applications in various fields are known and are described for example in U.S. Patents DB1 / 155183601.2 89Nos.9,187,538, 9,511,012, 9,517,191, 9,522,107, 9,522,108, 9,545,369, and 10,166,177, 10,287,728 and 10,301,768, all of which are incorporated herein in their entireties. Raw silk from silkworm Bombyx mori is composed of two primary proteins: silk fibroin (approximately 75%) and sericin (approximately 25%). Silk fibroin is a fibrous protein with a semi-crystalline structure that provides stiffness and strength. As used herein, the term “silk fibroin” means the fibers of the cocoon of Bombyx mori having a weight average molecular weight of about 370,000 Da. The crude silkworm fiber consists of a double thread of fibroin. The adhesive substance holding these double fibers together is sericin. The silk fibroin is composed of a heavy chain having a weight average molecular weight of about 350,000 Da (H chain), and a light chain having a weight average molecular weight about 25,000 Da (L chain). Silk fibroin is an amphiphilic polymer with large hydrophobic domains occupying the major component of the polymer, which has a high molecular weight. The hydrophobic regions are interrupted by small hydrophilic spacers, and the N- and C- termini of the chains are also highly hydrophilic. The hydrophobic domains of the H- chain contain a repetitive hexapeptide sequence of Gly-Ala-Gly-Ala-Gly-Ser and repeats of Gly-Ala / Ser / Tyr dipeptides, which can form stable anti-parallel-sheet crystallites. The amino acid sequence of the L-chain is non-repetitive, so the L-chain is more hydrophilic and relatively elastic. The hydrophilic (Tyr, Ser) and hydrophobic (Gly, Ala) chain segments in silk fibroin molecules are arranged alternatively such that allows self-assembling of silk fibroin molecules. As used herein, the term “fibroin” includes silk worm fibroin and insect or spider silk protein. In an embodiment, fibroin is obtained from Bombyx mori. Raw silk from Bombyx mori is composed of two primary proteins: silk fibroin (approximately 75%) and sericin (approximately 25%). As used herein, the term “silk fibroin” means the fibers of the cocoon of Bombyx mori having a weight average molecular weight of about 370,000 Da. Methods of making silk fibroin protein fragments, and / or compositions thereof, are known and are described for example in U.S. Patents Nos.9,187,538, 9,511,012, 9,517,191, 9,522,107, 9,522,108, 9,545,369, and 10,166,177. Recombinant silk described in patents and patent applications, incorporated by reference herein: US 2004590196, US 7,754,851, US 2007654470, US 7,951,908, US 2010785960, US 8,034,897, US 20090263430, US 2008226854, US 20090123967, US 2005712095, US 2007991037, US 20090162896, US 200885266, US 8,372,436, DB1 / 155183601.2 90US 2007989907, US 2009267596, US 2010319542, US 2009265344, US 2012684607, US 2004583227, US 8,030,024, US 2006643569, US 7,868,146, US 2007991916, US 8,097,583, US 2006643200, US 8,729,238, US 8,877,903, US 20190062557, US 20160280960, US 20110201783, US 2008991916, US 2011986662, US 2012697729, US 20150328363, US 9,034,816, US 20130172478, US 9,217,017, US 20170202995, US 8,721,991, US 2008227498, US 9,233,067, US 8,288,512, US 2008161364, US 7,148,039, US 1999247806, US 2001861597, US 2004887100, US 9,481,719, US 8,765,688, US 200880705, US 2010809102, US 8,367,803, US 2010664902, US 7,569,660, US 1999138833, US 2000591632, US 20120065126, US 20100278882, US 2008161352, US 20100015070, US 2009513709, US 20090194317, US 2004559286, US 200589551, US 2008187824, US 20050266242, US 20050227322, and US 20044418. Recombinant silk is also described in other patents and patent applications, incorporated by reference herein: US 20190062557, US 20150284565, US 20130225476, US 20130172478, US 20130136779, US 20130109762, US 20120252294, US 20110230911, US 20110201783, US 20100298877, US 10,478,520, US 10,253,213, US 10,072,152, US 9,233,067, US 9,217,017, US 9,034,816, US 8,877,903, US 8,729,238, US 8,721,991, US 8,097,583, US 8,034,897, US 8,030,024, US 7,951,908, US 7,868,146, and US 7,754,851. Recombinant silk protein and / or methods described herein, may include one or more recombinant silk proteins described above or recited in U.S. Patent Nos. 8,173,772, 8,278,416, 8,618,255, 8,642,734, 8,691,581, 8,729,235, 9,115,204, 9,157,070, 9,309,299, 9,644,012, 9,708,376, 9,051,453, 9,617,315, 9,968,682, 9,689,089, 9,732,125, 9,856,308, 9,926,348, 10,065,997, 10,316,069, and 10,329,332; and U.S. Patent Publication Nos.2009 / 0226969, 2011 / 0281273, 2012 / 0041177, 2013 / 0065278, 2013 / 0115698, 2013 / 0316376, 2014 / 0058066, 2014 / 0079674, 2014 / 0245923, 2015 / 0087046, 2015 / 0119554, 2015 / 0141618, 2015 / 0291673, 2015 / 0291674, 2015 / 0239587, 2015 / 0344542, 2015 / 0361144, 2015 / 0374833, 2015 / 0376247, 2016 / 0024464, 2017 / 0066804, 2017 / 0066805, 2015 / 0293076, 2016 / 0222174, 2017 / 0283474, 2017 / 0088675, 2019 / 0135880, 2015 / 0329587, 2019 / 0040109, 2019 / 0135881, 2019 / 0177363, 2019 / 0225646, 2019 / 0233481, 2019 / 0031842, 2018 / 0355120, 2019 / 0186050, 2019 / 0002644, 2020 / 0031887, 2018 / 0273590, 20191 / 094403, 2019 / 0031843, 2018 / 0251501, 2017 / 0066805, 2018 / 0127553, 2019 / 0329526, 2020 / 0031886, 2018 / 0080147, 2019 / 0352349, DB1 / 155183601.2 912020 / 0043085, 2019 / 0144819, 2019 / 0228449, 2019 / 0340666, 2020 / 0000091, 2019 / 0194710, 2019 / 0151505, 2018 / 0265555, 2019 / 0352330, 2019 / 0248847, and 2019 / 0378191, the entirety of which are incorporated herein by reference. As used herein, the term “substantially free of inorganic residuals” means that the composition exhibits residuals of 0.1 % (w / w) or less. In an embodiment, substantially free of inorganic residuals refers to a composition that exhibits residuals of 0.05% (w / w) or less. In an embodiment, substantially free of inorganic residuals refers to a composition that exhibits residuals of 0.01 % (w / w) or less. In an embodiment, the amount of inorganic residuals is between 0 ppm (“non-detectable” or “ND”) and 1000 ppm. In an embodiment, the amount of inorganic residuals is ND to about 500 ppm. In an embodiment, the amount of inorganic residuals is ND to about 400 ppm. In an embodiment, the amount of inorganic residuals is ND to about 300 ppm. In an embodiment, the amount of inorganic residuals is ND to about 200 ppm. In an embodiment, the amount of inorganic residuals is ND to about 100 ppm. In an embodiment, the amount of inorganic residuals is between 10 ppm and 1000 ppm. As used herein, the term “substantially free of organic residuals” means that the composition exhibits residuals of 0.1 % (w / w) or less, in an embodiment, substantially free of organic residuals refers to a composition that exhibits residuals of 0.05% (w / w) or less. In an embodiment, substantially free of organic residuals refers to a composition that exhibits residuals of 0.01% (w / w) or less. In an embodiment, the amount of organic residuals is between 0 ppm (“non-detectable” or “ND”) and 1000 ppm. In an embodiment, the amount of organic residuals is ND to about 500 ppm. In an embodiment, the amount of organic residuals is ND to about 400 ppm. In an embodiment, the amount of organic residuals is ND to about 300 ppm. In an embodiment, the amount of organic residuals is ND to about 200 ppm. In an embodiment, the amount of organic residuals is ND to about 100 ppm. In an embodiment, the amount of organic residuals is between 10 ppm and 1000 ppm. Compositions of the present disclosure exhibit “biocompatibility” meaning that the compositions are compatible with living tissue or a living system by not being toxic, injurious, or physiologically reactive and not causing immunological rejection. Such biocompatibility can be evidenced by participants topically applying compositions of the present disclosure on their skin for an extended period of time. In an embodiment, the extended period of time is about 3 days. In an embodiment, the extended period of time is about 7 days, in an embodiment, the extended period of DB1 / 155183601.2 92time is about 14 days, in an embodiment, the extended period of time is about 21 days. In an embodiment, the extended period of time is about 30 days. In an embodiment, the extended period of time is selected from the group consisting of about I month, about 2 months, about 3 months, about 4 months, about 5 months, about 6 months, about 7 months, about 8 months, about 9 months, about 10 months, about 11 months, about 12 months, and indefinitely. Compositions of the present disclosure can be “hypoallergenic” meaning that they are relatively unlikely to cause an allergic reaction. Such hypoallergenicity can be evidenced by participants topically applying compositions of the present disclosure on their skin for an extended period of time. In an embodiment, the extended period of time is about 3 days. In an embodiment, the extended period of time is about 7 days. In an embodiment, the extended period of time is about 14 days. In an embodiment, the extended period of time is about 21 days. In an embodiment, the extended period of time is about 30 days. In an embodiment, the extended period of time is selected from the group consisting of about 1 month, about 2 months, about 3 months, about 4 months, about 5 months, about 6 months, about 7 months, about 8 months, about 9 months, about 10 months, about 11 months, about 12 months, and indefinitely. In an embodiment, a peptide composition of the present disclosure has non- detectable levels of LiBr residuals. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is between 10 ppm and 1000 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is between 10 ppm and 300 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is less than 25 ppm. In an embodiment, the amount of the Li Br residuals in a composition of the present disclosure is less than 50 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is less than 75 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is less than 100 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is less than 200 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is less than 300 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is less than 400 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is less than 500 ppm. In an embodiment, the DB1 / 155183601.2 93amount of the LiBr residuals in a composition of the present disclosure is less than 600 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is less than 700 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is less than 800 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is less than 900 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is less than 1000 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is non- detectable to 500 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is non-detectable to 450 ppm. In an embodiment, the amount of the LiBr residue in a composition of the present disclosure is non-detectable to 400 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is non-detectable to 350 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is non-detectable to 300 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is non-detectable to 250 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is non-detectable to 200 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is non-detectable to 150 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is non-detectable to 100 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is 100 ppm to 200 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is 200 ppm to 300 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is 300 ppm to 400 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is 400 ppm to 500 ppm. In an embodiment, a peptide composition of the present disclosure has non- detectable levels of Na2CO3 residuals. In an embodiment, the amount of the Na2CO3 residuals in a composition of the present disclosure is less than 100 ppm. In an embodiment, the amount of the Na2CO3 residuals in a composition of the present disclosure is less than 200 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the present disclosure is less than 300 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the present DB1 / 155183601.2 94disclosure is less than 400 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the present disclosure is less than 500 ppm. In an embodiment, the amount of the Na2CO3 residuals in a composition of the present disclosure is less than 600 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the present disclosure is less than 700 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the present disclosure is less than 800 ppm. In an embodiment, the amount of the Na2CO3 residuals in a composition of the present disclosure is less than 900 ppm. In an embodiment, the amount of the Na2CO3 residuals in a composition of the present disclosure is less than 1000 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the present disclosure is non-detectable to 500 ppm. In an embodiment, the amount of the Na2CO3 residuals in a composition of the present disclosure is non-detectable to 450 ppm. In an embodiment, the amount of the Na2CO3 residuals in a composition of the present disclosure is non-detectable to 400 ppm. In an embodiment, the amount of the Na2CO3 residuals in a composition of the present disclosure is non-detectable to 350 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the present disclosure is non-detectable to 300 ppm. In an embodiment, the amount of the Na2CO3 residuals in a composition of the present disclosure is non-detectable to 250 ppm. In an embodiment, the amount of the Na2CO3 residuals in a composition of the present disclosure is non-detectable to 200 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the present disclosure is non-detectable to 150 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the present disclosure is non-detectable to 100 ppm. In an embodiment, the amount of the Na2CO3 residuals in a composition of the present disclosure is 100 ppm to 200 ppm. In an embodiment, the amount of the Na2CO3 residuals in a composition of the present disclosure is 200 ppm to 300 ppm. In an embodiment, the amount of the Na2CO3 residuals in a composition of the present disclosure is 300 ppm to 400 ppm. In an embodiment, the amount of the Na2CO3 residuals in a composition of the present disclosure is 400 ppm to 500 ppm. As used herein when referring to a number or a numerical range, the term “about” means that the stated number or numerical range is included together with numbers or numerical ranges within experimental variability, or within statistical experimental error from the stated number or numerical range, wherein the variation DB1 / 155183601.2 95or error is from 0% to 15%, or from 0% to 10%, or from 0% to 5% of the stated number or numerical range. The silk proteins or fragments thereof, silk solutions or mixtures (e.g., SPF or SFS solutions or mixture), and the like, may be prepared according to the methods described in U.S. Patent Nos.9,187,538, 9,522,107, 9,522,108, 9,511,012, 9,517,191, 9,545,369, and 10,166,177, and U.S. Patent Publication Nos.2016 / 0222579 and 2016 / 0281294, and International Patent Publication Nos. WO 2016 / 090055 and WO 2017 / 011679, the entirety of which are incorporated herein by reference. Methods of using silk fibroin or silk fibroin fragments in coating applications are known and are described for example in U.S. Patents Nos.10,287,728 and 10,301,768. The disclosure provides articles comprising coated fabrics, wherein the coating comprises a surfactant and / or emulsifier system and silk fibroin fragments, including modified silk fragments disclosed herein, e.g., and without limitation, fibroin peptides and / or protein fragments wherein at least one of the amino acids is modified, substituted, or replaced as disclosed herein and methods of making such articles. Silk is a natural polymer produced by a variety of insects and spiders. Silk produced by Bombyx mori (silkworm) comprises a filament core protein, silk fibroin, and a glue-like coating consisting of a nonfilamentous protein, sericin. Silk fibroin is a FDA approved, edible, non-toxic, and relative inexpensive silkworm cocoon derived proteins. The structure and content of amino acids in the silk fibroin protein are very similar to the tissue of the human body. Methods of making silk fibroin protein fragments are known and are described for example in U.S. Patents Nos.9,187,538, 9,511,012, 9,517,191, 9,522,107, 9,522,108, 9,545,369, and 10,166,177. Methods of using silk fibroin or silk fibroin fragments in coating applications, including coating applications of animal hair, are known and are described for example in U.S. Patent Application Publications Nos.20160222579, and 20160281294. Compositions and methods of using silk fibroin or silk fibroin fragments in cosmetic applications are known and are described for example in U.S. Patent Application Publications Nos. 20180280274 and 20180008522, and International Patent Application Publication No. WO 2019005848. All of the publications cited herein are incorporated by reference herein in their entireties. The recent advancements in silk material technology include the emergence of the top-down and bottom-up engineering of silk cocoon, specifically regenerate DB1 / 155183601.2 96cocoon into an aqueous silk solution and to use genetic engineering to produce recombinant silk with molecularly defined composition (Tran et al., A review of the emerging role of silk for the treatment of the eye, Pharm. Res., 2018, vol.35, pp.1- 16). In recent years, silk fibroin proteins are reported to have found applications in ocular tissue reconstruction, corneal tissue engineering and in ocular surface repair due to their biocompatibility, tunable properties, and transparency. Silk films have been found to support corneal cell growth and to develop stratified epithelial cell sheets equivalent to amniotic membrane substrates (Lawrence et al., Silk film biomaterials for cornea tissue engineering, Biomaterials, 2009; 30(7): 1299-308; Harkin et al., Silk fibroin in ocular tissue reconstruction, Biomaterials, 2011; Chirtla et al., Bombyx mori silk fibroin membranes as potential substrata for epithelial constructs used in the management of ocular surface disorders. Tissue Engineering Pan A, 2008; 14(7): 1203- 11.). Silk fibroin protein and hydrolyzed peptide fragments have been shown to inhibit transcription and upstream activation of NF-^B protein subunits and proinflammatory molecules that are classically under the control of NF- ^B (Hayden et al., Cell Research, 2011.21(2): 223-244; Chon et al., International Journal of Molecular Medicine, 2012.30(5): 1203-1210; Kim et al., J. Neurosurg., 2011.114(2): 485-90; J. Microbiol. Biotechnol., 2012.22(4): 494-500). Definitions As used in the preceding sections and throughout the rest of this specification, unless defined otherwise, all technical and scientific terms used herein have the same meaning as is commonly understood by one skilled in the art to which this disclosure belongs. All patents and publications referred to herein are incorporated by reference in their entireties. All percentages, parts and ratios are based upon the total weight of the eye care compositions of the present disclosure, unless otherwise specified. All such weights as they pertain to listed ingredients are based on the active level and, therefore, do not include solvents or by-products that may be included in commercially available materials, unless otherwise specified. The term “weight percent” may be denoted as “wt. %” or % w / w herein. As used herein, the term “a”, “an”, or “the” generally is construed to cover both the singular and the plural forms. DB1 / 155183601.2 97The term “about” as used herein, generally refers to a particular numeric value that is within an acceptable error range as determined by one of ordinary skill in the art, which will depend in part on how the numeric value is measured or determined, i.e., the limitations of the measurement system. For example, “about” can mean zero variation, and a range of ±20%, ±10%, or ±5% of a given numeric value. As used herein, the term “dermatologically acceptable carrier” means a carrier suitable for use in contact with mammalian keratinous tissue without causing any adverse effects such as undue toxicity, incompatibility, instability, allergic response, for example. A dermatologically acceptable carrier may include, without limitations, water, liquid or solid emollients, humectants, solvents, and the like. As used herein, the term “hydrophilic-lipophilic balance” (HLB) of a surfactant and / or emulsifier is a measure of the degree to which it is hydrophilic or hydrophobic, as determined by calculating values for the different regions of the molecule, as described by Griffin’s method HLB = 20 * Mh / M, where Mh is the molecular mass of the hydrophilic portion of the surfactant and / or emulsifier, and M is the molecular mass of the entire surfactant and / or emulsifier molecule, giving a result on a scale of 0 to 20. A HLB value of 0 corresponds to a completely lipophilic molecule, and a value of 20 corresponds to a completely hydrophilic molecule. The HLB value can be used to predict the surfactant and / or emulsifier properties of a molecule: HLB < 10: Lipid-soluble (water-insoluble), HLB >10: Water-soluble (lipid- insoluble), HLB = 1-3: anti-foaming agent, 3-6: W / O (water-in-oil) emulsifier, 7-9: wetting and spreading agent, 8-16: O / W (oil-in-water) emulsifier, 13-16: detergent, 16-18: solubilizer or hydrotrope. As used herein, “average weight average molecular weight” refers to an average of two or more values of weight average molecular weight of silk fibroin or fragments thereof of the same compositions, the two or more values determined by two or more separate experimental readings. As used herein, the term polymer “polydispersity (PD)” is generally used as a measure of the broadness of a molecular weight distribution of a polymer, and is ^^^^ defined by the formula polydispersity PD = ^^^^ . As used herein, the termhomogeneous” may refer to silk fibroin- based protein fragments that are distributed in a normal distribution about an identified molecular weight. As used herein, the term “substantially homogeneous” DB1 / 155183601.2 98may refer to an even distribution of a component or an additive, for example, silk fibroin fragments, dermatologically acceptable carrier, etc., throughout a composition of the present disclosure. As used herein, the terms “silk fibroin peptide,” “silk fibroin protein fragment,” and “silk fibroin fragment” are used interchangeably. Molecular weight or number of amino acids units are defined when molecular size becomes an important parameter. As used herein, the term “fast-dissolving solid forms” refers to fast-dissolving solid forms including freeze dried forms (cakes, wafers, thin films), and compressed tablets. As used herein, the terms “peptide” or “protein” refers to a chain of amino acids that are held together by peptide bonds (also called amide bonds). The basic distinguishing factors for proteins and peptides are size and structure. Peptides are smaller than proteins. Traditionally, peptides are defined as molecules that consist of between 2 and 50 amino acids, whereas proteins are made up of 50 or more amino acids. In addition, peptides tend to be less well defined in structure than proteins, which can adopt complex conformations known as secondary, tertiary, and quaternary structures. As used herein, the term “fibroin” or “silk protein” is a type of structural protein produced by certain spider and insect species that produce silk (See definition provided in WIPO Pearl-WIPO’s Multilingual Terminology Portal database, https: / / wipopearl.wipo.int / en / linguistic). Fibroin may include silkworm fibroin, insect or spider silk protein (e.g., spidroin), recombinant spider protein, silk proteins present in other spider silk types, e.g., tubuliform silk protein (TuSP), flagelliform silk protein, minor ampullate silk proteins, aciniform silk protein, pyriform silk protein, aggregate silk glue), silkworm fibroin produced by genetically modified silkworm, or recombinant silkworm fibroin. As used herein, the term “silk fibroin” refers to silkworm fibroin, silk fibroin produced by genetically modified silkworm, or recombinant silkworm fibroin (See (1) Narayan Ed., Encyclopedia of Biomedical Engineering, Vol.2, Elsevier, 2019; (2) Kobayashi et al. Eds, Encyclopedia of Polymeric Nanomaterials, Springer, 2014, https: / / link.springer.com / referenceworkentry / 10.1007%2F978-3-642-36199-9_323-1). In an embodiment, silk fibroin is obtained from Bombyx mori. DB1 / 155183601.2 99The term “solid solution” as used herein, refers to the active agent molecularly dissolved in the solid excipient matrix such as hydrophobic polymers, wherein the active agent is miscible with the polymer matrix excipient. The term “solid dispersion” as used herein, refers to the active agent dispersed as crystalline or amorphous particles, wherein the active agent is dispersed in an amorphous polymer and is distributed at random between the polymer matrix excipient. As used herein, the term “substantially homogeneous” may refer to silk fibroin-based protein fragments that are distributed in a normal distribution about an identified molecular weight. As used herein, the term “substantially homogeneous” may also refer to an even distribution of a component or an additive, for example, silk fibroin-based protein fragments, dermatologically acceptable carrier, etc., throughout the silk eye care composition. As used herein, the term “surface tension” refers to the tendency of fluid surfaces to shrink into the minimum surface area possible. At liquid–air interfaces, surface tension results from the greater attraction of liquid molecules to each other (due to cohesion) than to the molecules in the air (due to adhesion). The net effect is an inward force at its surface that causes the liquid to behave as if its surface were covered with a stretched elastic membrane. Because of the relatively high attraction of water molecules to each other through a web of hydrogen bonds, water has a higher surface tension (72.8 mN / m at 20 °C) than most other liquids. SPF Definitions and Properties As used herein, “silk protein fragments” (SPF) include, without limitation, one or more of: “silk fibroin fragments” as defined herein; “recombinant silk fragments” as defined herein; “spider silk fragments” as defined herein; “silk fibroin-like protein fragments” as defined herein; “chemically modified silk fragments” as defined herein; “sericin or sericin fragments” as defined herein; and / or modified silk fragments disclosed herein, e.g., and without limitation, fibroin peptides and / or protein fragments wherein at least one of the amino acids is modified, substituted, or replaced as disclosed herein. SPF may have any molecular weight values or ranges described herein, and any polydispersity values or ranges described herein. DB1 / 155183601.2 100SPF Molecular Weight and Polydispersity In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 1 to about 5 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 5 to about 10 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 10 to about 15 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 15 to about 20 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 14 to about 30 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 20 to about 25 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 25 to about 30 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 30 to about 35 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 35 to about 40 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 39 to about 54 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 40 to about 45 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 45 to about 50 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 50 to about 55 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 55 to about 60 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 60 to about 65 kDa. In an embodiment, a composition of the DB1 / 155183601.2 101present disclosure includes SPF having an average weight average molecular weight selected from between about 65 to about 70 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 70 to about 75 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 75 to about 80 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 80 to about 85 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 85 to about 90 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 90 to about 95 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 95 to about 100 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 100 to about 105 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 105 to about 110 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 110 to about 115 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 115 to about 120 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 120 to about 125 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 125 to about 130 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 130 to about 135 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 135 to about 140 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 140 to about 145 kDa. In an embodiment, a composition of the present disclosure includes DB1 / 155183601.2 102SPF having an average weight average molecular weight selected from between about 145 to about 150 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 150 to about 155 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 155 to about 160 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 160 to about 165 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 165 to about 170 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 170 to about 175 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 175 to about 180 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 180 to about 185 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 185 to about 190 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 190 to about 195 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 195 to about 200 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 200 to about 205 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 205 to about 210 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 210 to about 215 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 215 to about 220 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 220 to about 225 kDa. In an embodiment, a composition of the present disclosure includes DB1 / 155183601.2 103SPF having an average weight average molecular weight selected from between about 225 to about 230 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 230 to about 235 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 235 to about 240 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 240 to about 245 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 245 to about 250 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 250 to about 255 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 255 to about 260 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 260 to about 265 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 265 to about 270 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 270 to about 275 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 275 to about 280 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 280 to about 285 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 285 to about 290 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 290 to about 295 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 295 to about 300 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 300 to about 305 kDa. In an embodiment, a composition of the present disclosure includes DB1 / 155183601.2 104SPF having an average weight average molecular weight selected from between about 305 to about 310 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 310 to about 315 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 315 to about 320 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 320 to about 325 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 325 to about 330 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 330 to about 335 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 335 to about 340 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 340 to about 345 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 345 to about 350 kDa. In some embodiments, compositions of the present disclosure include SPF compositions selected from compositions #1001 to #2450, having weight average molecular weights selected from about 1 kDa to about 145 kDa, and a polydispersity selected from between 1 and about 5 (including, without limitation, a polydispersity of 1), between 1 and about 1.5 (including, without limitation, a polydispersity of 1), between about 1.5 and about 2, between about 1.5 and about 3, between about 2 and about 2.5, between about 2.5 and about 3, between about 3 and about 3.5, between about 3.5 and about 4, between about 4 and about 4.5, and between about 4.5 and about 5: PDI -500DB1 / 155183601.2 1053 kDa 1021 1022 1023 1024 1025 1026 1027 1028 1029 10304 kDa 1031 1032 1033 1034 1035 1036 1037 1038 1039 1040500000000000000000000000000000000000000000000DB1 / 155183601.2 10649 kDa 1481 1482 1483 1484 1485 1486 1487 1488 1489 149050 kDa 1491 1492 1493 1494 1495 1496 1497 1498 1499 1500100000000000000000000000000000000000000000000DB1 / 155183601.2 10795 kDa 1941 1942 1943 1944 1945 1946 1947 1948 1949 195096 kDa 1951 1952 1953 1954 1955 1956 1957 1958 1959 1960700000000000000000000000000000000000000000000DB1 / 155183601.2 108141 kDa 2401 2402 2403 2404 2405 2406 2407 2408 2409 2410142 kDa 2411 2412 2413 2414 2415 2416 2417 2418 2419 24203000polydispersity selected from between 1 to about 5.0, including, without limitation, a polydispersity of 1. In an embodiment, SPF in a composition of the present disclosure have a polydispersity selected from between about 1.5 to about 3.0. In an embodiment, SPF in a composition of the present disclosure have a polydispersity selected from between 1 to about 1.5, including, without limitation, a polydispersity of 1. In an embodiment, SPF in a composition of the present disclosure have a polydispersity selected from between about 1.5 to about 2.0. In an embodiment, SPF in a composition of the present disclosure have a polydispersity selected from between about 2.0 to about 2.5. In an embodiment, SPF in a composition of the present disclosure have a polydispersity selected from between about 2.5 to about 3.0. In an embodiment, SPF in a composition of the present disclosure have a polydispersity selected from between about 3.0 to about 3.5. In an embodiment, SPF in a composition of the present disclosure have a polydispersity selected from between about 3.5 to about 4.0. In an embodiment, SPF in a composition of the present disclosure have a polydispersity selected from between about 4.0 to about 4.5. In an embodiment, SPF in a composition of the present disclosure have a polydispersity selected from between about 4.5 to about 5.0. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of 1. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 1.1. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 1.2. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 1.3. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 1.4. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 1.5. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 1.6. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 1.7. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of DB1 / 155183601.2 109about 1.8. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 1.9. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 2.0. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 2.1. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 2.2. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 2.3. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 2.4. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 2.5. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 2.6. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 2.7. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 2.8. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 2.9. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 3.0. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 3.1. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 3.2. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 3.3. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 3.4. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 3.5. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 3.6. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 3.7. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 3.8. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 3.9. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 4.0. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 4.1. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 4.2. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 4.3. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 4.4. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 4.5. In an DB1 / 155183601.2 110embodiment, SPF in a composition of the present disclosure have a polydispersity of about 4.6. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 4.7. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 4.8. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 4.9. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 5.0. Silk Fibroin Fragments Methods of making silk fibroin or silk fibroin protein fragments and their applications in various fields are known and are described for example in U.S. Patents Nos.9,187,538, 9,511,012, 9,517,191, 9,522,107, 9,522,108, 9,545,369, and 10,166,177, 10,287,728 and 10,301,768, all of which are incorporated herein in their entireties. Raw silk from silkworm Bombyx mori is composed of two primary proteins: silk fibroin (approximately 75%) and sericin (approximately 25%). Silk fibroin is a fibrous protein with a semi-crystalline structure that provides stiffness and strength. As used herein, the term “silk fibroin” means the fibers of the cocoon of Bombyx mori having a weight average molecular weight of about 370,000 Da. The crude silkworm fiber consists of a double thread of fibroin. The adhesive substance holding these double fibers together is sericin. The silk fibroin is composed of a heavy chain having a weight average molecular weight of about 350,000 Da (H chain), and a light chain having a weight average molecular weight about 25,000 Da (L chain). Silk fibroin is an amphiphilic polymer with large hydrophobic domains occupying the major component of the polymer, which has a high molecular weight. The hydrophobic regions are interrupted by small hydrophilic spacers, and the N- and C- termini of the chains are also highly hydrophilic. The hydrophobic domains of the H- chain contain a repetitive hexapeptide sequence of Gly-Ala-Gly-Ala-Gly-Ser and repeats of Gly-Ala / Ser / Tyr dipeptides, which can form stable anti-parallel-sheet crystallites. The amino acid sequence of the L-chain is non-repetitive, so the L-chain is more hydrophilic and relatively elastic. The hydrophilic (Tyr, Ser) and hydrophobic (Gly, Ala) chain segments in silk fibroin molecules are arranged alternatively such that allows self-assembling of silk fibroin molecules. Provided herein are methods for producing pure and highly scalable silk fibroin-protein fragment mixture solutions that may be used across multiple industries DB1 / 155183601.2 111for a variety of applications. Without wishing to be bound by any particular theory, it is believed that these methods are equally applicable to fragmentation of any SPF described herein, including without limitation recombinant silk proteins, and fragmentation of silk-like or fibroin-like proteins. As used herein, the term “fibroin” includes silk worm fibroin and insect or spider silk protein. In an embodiment, fibroin is obtained from Bombyx mori. Raw silk from Bombyx mori is composed of two primary proteins: silk fibroin (approximately 75%) and sericin (approximately 25%). Silk fibroin is a fibrous protein with a semi-crystalline structure that provides stiffness and strength. As used herein, the term “silk fibroin” means the fibers of the cocoon of Bombyx mori having a weight average molecular weight of about 370,000 Da. Conversion of these insoluble silk fibroin fibrils into water-soluble silk fibroin protein fragments requires the addition of a concentrated neutral salt (e.g., 8-10 M lithium bromide), which interferes with inter- and intramolecular ionic and hydrogen bonding that would otherwise render the fibroin protein insoluble in water. Methods of making silk fibroin protein fragments, and / or compositions thereof, are known and are described for example in U.S. Patents Nos.9,187,538, 9,511,012, 9,517,191, 9,522,107, 9,522,108, 9,545,369, and 10,166,177. FIG.73 is a flow chart showing generic embodiments for producing some embodiments of the present disclosure. It should be understood that not all of the steps illustrated are necessarily required to fabricate all silk solutions of the present disclosure. As illustrated in FIG.74, step A, cocoons (heat-treated or non-heat- treated), silk fibers, silk powder, spider silk or recombinant spider silk can be used as the silk source. FIG.74 is a flow chart showing generic embodiments for various parameters that can be modified during the process of producing a silk protein fragment solution of the present disclosure during the extraction and the dissolution steps. In some embodiments, specifically disclosed herein, molecular weight of the silk fibroin protein fragment composition was determined using High Pressure Liquid Chromatography (HPLC) with a Refractive Index Detector (RID), and polydispersity was calculated using Cirrus GPC Online GPC / SEC Software Version 3.3 (Agilent). In some embodiments specifically disclosed herein, molecular weight of the silk fibroin protein fragment composition was determined using SEC-MALS methods. DB1 / 155183601.2 112Data analysis and calculations – Calculation of Average Molecular Weight using Cirrus Software Upload the chromatography data files of the standards and the analytical samples into Cirrus SEC data collection and molecular weight analysis software. Calculate the weight average molecular weight (Mw), number average molecular weight (Mn), peak average molecular weight (Mp), and polydispersity for each injection of the sample. Spider Silk Fragments Spider silks are natural polymers that consist of three domains: a repetitive middle core domain that dominates the protein chain, and non-repetitive N-terminal and C-terminal domains. The large core domain is organized in a block copolymer- like arrangement, in which two basic sequences, crystalline [poly(A) or poly(GA)] and less crystalline (GGX or GPGXX) polypeptides alternate. Dragline silk is the protein complex composed of major ampullate dragline silk protein 1 (MaSp1) and major ampullate dragline silk protein 2 (MaSp2). Both silks are approximately 3500 amino acid long. MaSp1 can be found in the fiber core and the periphery, whereas MaSp2 forms clusters in certain core areas. The large central domains of MaSp1 and MaSp2 are organized in block copolymer-like arrangements, in which two basic sequences, crystalline [poly(A) or poly(GA)] and less crystalline (GGX or GPGXX) polypeptides alternate in core domain. Specific secondary structures have been assigned to poly(A) / (GA), GGX and GPGXX motifs including β-sheet, α-helix and β-spiral respectively. The primary sequence, composition and secondary structural elements of the repetitive core domain are responsible for mechanical properties of spider silks; whereas, non-repetitive N- and C-terminal domains are essential for the storage of liquid silk dope in a lumen and fiber formation in a spinning duct. The main difference between MaSp1 and MaSp2 is the presence of proline (P) residues accounting for 15% of the total amino acid content in MaSp2, whereas MaSp1 is proline-free. By calculating the number of proline residues in N. clavipes dragline silk, it is possible to estimate the presence of the two proteins in fibers; 81% MaSp1 and 19% MaSp2. Different spiders have different ratios of MaSp1 and MaSp2. For example, a dragline silk fiber from the orb weaver Argiope aurantia contains 41% MaSp1 and 59% MaSp2. Such changes in the ratios of major ampullate silks can dictate the performance of the silk fiber. DB1 / 155183601.2 113At least seven different types of silk proteins are known for one orb-weaver species of spider. Silks differ in primary sequence, physical properties and functions. For example, dragline silks used to build frames, radii and lifelines are known for outstanding mechanical properties including strength, toughness and elasticity. On an equal weight basis, spider silk has a higher toughness than steel and Kevlar. Flageliform silk found in capture spirals has extensibility of up to 500%. Minor ampullate silk, which is found in auxiliary spirals of the orb-web and in prey wrapping, possesses high toughness and strength almost similar to major ampullate silks, but does not supercontract in water. Spider silks are known for their high tensile strength and toughness. The recombinant silk proteins also confer advantageous properties to cosmetic or dermatological compositions, in particular to be able to improve the hydrating or softening action, good film forming property and low surface density. Diverse and unique biomechanical properties together with biocompatibility and a slow rate of degradation make spider silks excellent candidates as biomaterials for tissue engineering, guided tissue repair and drug delivery, for cosmetic products (e.g. nail and hair strengthener, skin care products), and industrial materials (e.g. nanowires, nanofibers, surface coatings). In an embodiment, a silk protein may include a polypeptide derived from natural spider silk proteins. The polypeptide is not limited particularly as long as it is derived from natural spider silk proteins, and examples of the polypeptide include natural spider silk proteins and recombinant spider silk proteins such as variants, analogs, derivatives or the like of the natural spider silk proteins. In terms of excellent tenacity, the polypeptide may be derived from major dragline silk proteins produced in major ampullate glands of spiders. Examples of the major dragline silk proteins include major ampullate spidroin MaSp1 and MaSp2 from Nephila clavipes, and ADF3 and ADF4 from Araneus diadematus, etc. Examples of the polypeptide derived from major dragline silk proteins include variants, analogs, derivatives or the like of the major dragline silk proteins. Further, the polypeptide may be derived from flagelliform silk proteins produced in flagelliform glands of spiders. Examples of the flagelliform silk proteins include flagelliform silk proteins derived from Nephila clavipes, etc. Examples of the polypeptide derived from major dragline silk proteins include a polypeptide containing two or more units of an amino acid sequence represented by DB1 / 155183601.2 114the formula 1: REP1-REP2 (1), preferably a polypeptide containing five or more units thereof, and more preferably a polypeptide containing ten or more units thereof. Alternatively, the polypeptide derived from major dragline silk proteins may be a polypeptide that contains units of the amino acid sequence represented by the formula 1: REP1-REP2 (1) and that has, at a C-terminal, an amino acid sequence represented by any of SEQ ID NOS: 1 to 3 of U.S. Patent No.9,051,453, which is incorporated by reference herein in its entirety, or an amino acid sequence having a homology of 90% or more with the amino acid sequence represented by any of SEQ ID NOS: 1 to 3 of U.S. Patent No.9,051,453, which is incorporated by reference herein in its entirety. In the polypeptide derived from major dragline silk proteins, units of the amino acid sequence represented by the formula 1: REP1-REP2 (1) may be the same or may be different from each other. In the case of producing a recombinant protein using a microbe such as Escherichia coli as a host, the molecular weight of the polypeptide derived from major dragline silk proteins is 500 kDa or less, or 300 kDa or less, or 200 kDa or less, in terms of productivity. In the formula (1), the REP1 indicates polyalanine. In the REP1, the number of alanine residues arranged in succession is preferably 2 or more, more preferably 3 or more, further preferably 4 or more, and particularly preferably 5 or more. Further, in the REP1, the number of alanine residues arranged in succession is preferably 20 or less, more preferably 16 or less, further preferably 12 or less, and particularly preferably 10 or less. In the formula (1), the REP2 is an amino acid sequence composed of 10 to 200 amino acid residues. The total number of glycine, serine, glutamine and alanine residues contained in the amino acid sequence is 40% or more, preferably 60% or more, and more preferably 70% or more with respect to the total number of amino acid residues contained therein. In the major dragline silk, the REP1 corresponds to a crystal region in a fiber where a crystal β sheet is formed, and the REP2 corresponds to an amorphous region in a fiber where most of the parts lack regular configurations and that has more flexibility. Further, the [REP1-REP2] corresponds to a repetitious region (repetitive sequence) composed of the crystal region and the amorphous region, which is a characteristic sequence of dragline silk proteins. DB1 / 155183601.2 115Recombinant Silk Fragments In some embodiments, the recombinant silk protein refers to recombinant spider silk polypeptides, recombinant insect silk polypeptides, or recombinant mussel silk polypeptides. In some embodiments, the recombinant silk protein fragment disclosed herein include recombinant spider silk polypeptides of Araneidae or Araneoids, or recombinant insect silk polypeptides of Bombyx mori. In some embodiments, the recombinant silk protein fragment disclosed herein include recombinant spider silk polypeptides of Araneidae or Araneoids. In some embodiments, the recombinant silk protein fragment disclosed herein include block copolymer having repetitive units derived from natural spider silk polypeptides of Araneidae or Araneoids. In some embodiments, the recombinant silk protein fragment disclosed herein include block copolymer having synthetic repetitive units derived from spider silk polypeptides of Araneidae or Araneoids and non-repetitive units derived from natural repetitive units of spider silk polypeptides of Araneidae or Araneoids. Recent advances in genetic engineering have provided a route to produce various types of recombinant silk proteins. Recombinant DNA technology has been used to provide a more practical source of silk proteins. As used herein “recombinant silk protein” refers to synthetic proteins produced heterologously in prokaryotic or eukaryotic expression systems using genetic engineering methods. Various methods for synthesizing recombinant silk peptides are known and have been described by Ausubel et al., Current Protocols in Molecular Biology § 8 (John Wiley & Sons 1987, (1990)), incorporated herein by reference. A gram- negative, rod-shaped bacterium E. coli is a well-established host for industrial scale production of proteins. Therefore, the majority of recombinant silks have been produced in E. coli. E. coli which is easy to manipulate, has a short generation time, is relatively low cost and can be scaled up for larger amounts protein production. The recombinant silk proteins can be produced by transformed prokaryotic or eukaryotic systems containing the cDNA coding for a silk protein, for a fragment of this protein or for an analog of such a protein. The recombinant DNA approach enables the production of recombinant silks with programmed sequences, secondary structures, architectures and precise molecular weight. There are four main steps in the process: (i) design and assembly of synthetic silk-like genes into genetic DB1 / 155183601.2 116‘cassettes’, (ii) insertion of this segment into a DNA recombinant vector, (iii) transformation of this recombinant DNA molecule into a host cell and (iv) expression and purification of the selected clones. The term “recombinant vectors”, as used herein, includes any vectors known to the skilled person including plasmid vectors, cosmid vectors, phage vectors such as lambda phage, viral vectors such as adenoviral or baculoviral vectors, or artificial chromosome vectors such as bacterial artificial chromosomes (BAC), yeast artificial chromosomes (YAC), or P1 artificial chromosomes (PAC). Said vectors include expression as well as cloning vectors. Expression vectors comprise plasmids as well as viral vectors and generally contain a desired coding sequence and appropriate DNA sequences necessary for the expression of the operably linked coding sequence in a particular host organism (e.g., bacteria, yeast, or plant) or in in vitro expression systems. Cloning vectors are generally used to engineer and amplify a certain desired DNA fragment and may lack functional sequences needed for expression of the desired DNA fragments. The prokaryotic systems include Gram-negative bacteria or Gram-positive bacteria. The prokaryotic expression vectors can include an origin of replication which can be recognized by the host organism, a homologous or heterologous promoter which is functional in the said host, the DNA sequence coding for the spider silk protein, for a fragment of this protein or for an analogous protein. Nonlimiting examples of prokaryotic expression organisms are Escherichia coli, Bacillus subtilis, Bacillus megaterium, Corynebacterium glutamicum, Anabaena, Caulobacter, Gluconobacter, Rhodobacter, Pseudomonas, Para coccus, Bacillus (e.g. Bacillus subtilis) Brevibacterium, Corynebacterium, Rhizobium (Sinorhizobium), Flavobacterium, Klebsiella, Enterobacter, Lactobacillus, Lactococcus, Methylobacterium, Propionibacterium, Staphylococcus or Streptomyces cells. The eukaryotic systems include yeasts and insect, mammalian or plant cells. In this case, the expression vectors can include a yeast plasmid origin of replication or an autonomous replication sequence, a promoter, a DNA sequence coding for a spider silk protein, for a fragment or for an analogous protein, a polyadenylation sequence, a transcription termination site and, lastly, a selection gene. Nonlimiting examples of eukaryotic expression organisms include yeasts, such as Saccharomyces cerevisiae, Pichia pastoris, basidiosporogenous, ascosporogenous, filamentous fungi, such as Aspergillus niger, Aspergillus oryzae, Aspergillus nidulans, Trichoderma reesei, DB1 / 155183601.2 117Acremonium chrysogenum, Candida, Hansenula, Kluyveromyces, Saccharomyces (e.g. Saccharomyces cerevisiae), Schizosaccharomyces, Pichia (e.g. Pichia pastoris) or Yarrowia cells etc., mammalian cells, such as HeLa cells, COS cells, CHO cells etc., insect cells, such as Sf9 cells, MEL cells, etc., “insect host cells” such as Spodoptera frugiperda or Trichoplusia ni cells. SF9 cells, SF-21 cells or High-Five cells, wherein SF-9 and SF-21 are ovarian cells from Spodoptera frugiperda, and High-Five cells are egg cells from Trichoplusia ni., “plant host cells”, such as tobacco, potato or pea cells. A variety of heterologous host systems have been explored to produce different types of recombinant silks. Recombinant partial spidroins as well as engineered silks have been cloned and expressed in bacteria (Escherichia coli), yeast (Pichia pastoris), insects (silkworm larvae), plants (tobacco, soybean, potato, Arabidopsis), mammalian cell lines (BHT / hamster) and transgenic animals (mice, goats). Most of the silk proteins are produced with an N- or C-terminal His-tags to make purification simple and produce enough amounts of the protein. In some embodiments, the host suitable for expressing the recombinant spider silk protein using heterogeneous system may include transgenic animals and plants. In some embodiments, the host suitable for expressing the recombinant spider silk protein using heterogeneous system comprises bacteria, yeasts, mammalian cell lines. In some embodiments, the host suitable for expressing the recombinant spider silk protein using heterogeneous system comprises E. coli. In some embodiments, the host suitable for expressing the recombinant spider silk protein using heterogeneous system comprises transgenic B. mori silkworm generated using genome editing technologies (e.g., CRISPR). The recombinant silk protein in this disclosure comprises synthetic proteins which are based on repeat units of natural silk proteins. Besides the synthetic repetitive silk protein sequences, these can additionally comprise one or more natural nonrepetitive silk protein sequences. In some embodiments, “recombinant silk protein” refers to recombinant silkworm silk protein or fragments thereof. The recombinant production of silk fibroin and silk sericin has been reported. A variety of hosts are used for the production including E. coli, Sacchromyces cerevisiae, Pseudomonas sp., Rhodopseudomonas sp., Bacillus sp., and Strepomyces. See EP 0230702, which is incorporate by reference herein by its entirety. DB1 / 155183601.2 118Provided herein also include design and biological-synthesis of silk fibroin protein-like multiblock polymer comprising GAGAGX hexapeptide (X is A, Y, V or S) derived from the repetitive domain of B. mori silk heavy chain (H chain) In some embodiments, this disclosure provides silk protein-like multiblock polymers derived from the repetitive domain of B. mori silk heavy chain (H chain) comprising the GAGAGS hexapeptide repeating units. The GAGAGS hexapeptide is the core unit of H-chain and plays an important role in the formation of crystalline domains. The silk protein-like multiblock polymers containing the GAGAGS hexapeptide repeating units spontaneously aggregate into β-sheet structures, similar to natural silk fibroin protein, where in the silk protein-like multiblock polymers having any weight average molecular weight described herein. In some embodiments, this disclosure provides silk-peptide like multiblock copolymers composed of the GAGAGS hexapeptide repetitive fragment derived from H chain of B. mori silk heavy chain and mammalian elastin VPGVG motif produced by E. coli. In some embodiments, this disclosure provides fusion silk fibroin proteins composed of the GAGAGS hexapeptide repetitive fragment derived from H chain of B. mori silk heavy chain and GVGVP produced by E. coli, where in the silk protein- like multiblock polymers having any weight average molecular weight described herein. In some embodiments, this disclosure provides B. mori silkworm recombinant proteins composed of the (GAGAGS)16repetitive fragment. In some embodiments, this disclosure provides recombinant proteins composed of the (GAGAGS)16 repetitive fragment and the non-repetitive (GAGAGS)16–F-COOH, (GAGAGS)16–F- F-COOH, (GAGAGS)16 –F-F-F-COOH, (GAGAGS)16 –F-F-F-F-COOH, (GAGAGS)16–F-F-F-F-F-F-F-F-COOH, (GAGAGS)16–F-F-F-F–F-F-F-F-F-F-F-F- COOH produced by E. coli, where F has the following amino acid sequence SGFGPVANGGSGEASSESDFGSSGFGPVANASSGEASSESDFAG, and where in the silk protein-like multiblock polymers having any weight average molecular weight described herein. In some embodiments, “recombinant silk protein” refers to recombinant spider silk protein or fragments thereof. The productions of recombinant spider silk proteins based on a partial cDNA clone have been reported. The recombinant spider silk proteins produced as such comprise a portion of the repetitive sequence derived from a dragline spider silk protein, Spidroin 1, from the spider Nephila clavipes. see Xu et DB1 / 155183601.2 119al. (Proc. Natl. Acad. Sci. U.S.A., 87:7120–7124 (1990). cDNA clone encoding a portion of the repeating sequence of a second fibroin protein, Spidroin 2, from dragline silk of Nephila clavipes and the recombinant synthesis thereof is described in J. Biol. Chem., 1992, volume 267, pp.19320–19324. The recombinant synthesis of spider silk proteins including protein fragments and variants of Nephila clavipes from transformed E. coli is described in U.S. Pat. Nos.5,728,810 and 5,989,894. cDNA clones encoding minor ampullate spider silk proteins and the expression thereof is described in U.S. Pat. Nos.5,733,771 and 5,756,677. cDNA clone encoding the flagelliform silk protein from an orb-web spinning spider is described in U.S. Pat. No. 5,994,099. U.S. Pat. No.6,268,169 describes the recombinant synthesis of spider silk like proteins derived from the repeating peptide sequence found in the natural spider dragline of Nephila clavipes by E. coli, Bacillus subtilis, and Pichia pastoris recombinant expression systems. WO 03 / 020916 describes the cDNA clone encoding and recombinant production of spider spider silk proteins having repeative sequences derived from the major ampullate glands of Nephila madagascariensis, Nephila senegalensis, Tetragnatha kauaiensis, Tetragnatha versicolor, Argiope aurantia, Argiope trifasciata, Gasteracantha mammosa, and Latrodectus geometricus, the flagelliform glands of Argiope trifasciata, the ampullate glands of Dolomedes tenebrosus, two sets of silk glands from Plectreurys tristis, and the silk glands of the mygalomorph Euagrus chisoseus. Each of the above reference is incorporated herein by reference in its entirety. In some embodiments, the recombinant spider silk protein is a hybrid protein of a spider silk protein and an insect silk protein, a spider silk protein and collagen, a spider silk protein and resilin, or a spider silk protein and keratin. The spider silk repetitive unit comprises or consists of an amino acid sequence of a region that comprises or consists of at least one peptide motif that repetitively occurs within a naturally occurring major ampullate gland polypeptide, such as a dragline spider silk polypeptide, a minor ampullate gland polypeptide, a flagelliform polypeptide, an aggregate spider silk polypeptide, an aciniform spider silk polypeptide or a pyriform spider silk polypeptide. In some embodiments, the recombinant spider silk protein in this disclosure comprises synthetic spider silk proteins derived from repetitive units of natural spider silk proteins, consensus sequence, and optionally one or more natural non-repetitive spider silk protein sequences. The repeated units of natural spider silk polypeptide DB1 / 155183601.2 120may include dragline spider silk polypeptides or flagelliform spider silk polypeptides of Araneidae or Araneoids. As used herein, the spider silk “repetitive unit” comprises or consists of at least one peptide motif that repetitively occurs within a naturally occurring major ampullate gland polypeptide, such as a dragline spider silk polypeptide, a minor ampullate gland polypeptide, a flagelliform polypeptide, an aggregate spider silk polypeptide, an aciniform spider silk polypeptide or a pyriform spider silk polypeptide. A “repetitive unit” refers to a region which corresponds in amino acid sequence to a region that comprises or consists of at least one peptide motif (e.g. AAAAAA or GPGQQ) that repetitively occurs within a naturally occurring silk polypeptide (e.g. MaSpI, ADF-3, ADF-4, or Flag) (i.e. identical amino acid sequence) or to an amino acid sequence substantially similar thereto (i.e. variational amino acid sequence). A “repetitive unit” having an amino acid sequence which is “substantially similar” to a corresponding amino acid sequence within a naturally occurring silk polypeptide (i.e. wild-type repetitive unit) is also similar with respect to its properties, e.g. a silk protein comprising the “substantially similar repetitive unit” is still insoluble and retains its insolubility. A “repetitive unit” having an amino acid sequence which is “identical” to the amino acid sequence of a naturally occurring silk polypeptide, for example, can be a portion of a silk polypeptide corresponding to one or more peptide motifs of MaSpI, MaSpII, ADF-3 and / or ADF-4. A “repetitive unit” having an amino acid sequence which is “substantially similar” to the amino acid sequence of a naturally occurring silk polypeptide, for example, can be a portion of a silk polypeptide corresponding to one or more peptide motifs of MaSpI, MaSpII, ADF-3 and / or ADF-4, but having one or more amino acid substitution at specific amino acid positions. As used herein, the term “consensus peptide sequence” refers to an amino acid sequence which contains amino acids which frequently occur in a certain position (e.g. “G”) and wherein, other amino acids which are not further determined are replaced by the place holder “X”. In some embodiments, the consensus sequence is at least one of (i) GPGXX, wherein X is an amino acid selected from A, S, G, Y, P and Q; (ii) GGX, wherein X is an amino acid selected from Y, P, R, S, A, T, N and Q, preferably Y, P and Q; (iii) Ax, wherein x is an integer from 5 to 10. The consensus peptide sequences GPGXX and GGX, i.e. glycine rich motifs, provide flexibility to the silk polypeptide and thus, to the thread formed from the silk DB1 / 155183601.2 121protein containing said motifs. In detail, the iterated GPGXX motif forms turn spiral structures, which imparts elasticity to the silk polypeptide. Major ampullate and flagelliform silks both have a GPGXX motif. The iterated GGX motif is associated with a helical structure having three amino acids per turn and is found in most spider silks. The GGX motif may provide additional elastic properties to the silk. The iterated polyalanine Ax (peptide) motif forms a crystalline β-sheet structure that provides strength to the silk polypeptide, as described for example in WO 03 / 057727. In some embodiments, the recombinant spider silk protein in this disclosure comprises two identical repetitive units each comprising at least one, preferably one, amino acid sequence selected from the group consisting of: GGRPSDTYG and GGRPSSSYG derived from Resilin. Resilin is an elastomeric protein found in most arthropods that provides low stiffness and high strength. As used herein, “non-repetitive units” refers to an amino acid sequence which is “substantially similar” to a corresponding non-repetitive (carboxy ...

Claims

CLAIMS 1. A composition comprising a plurality of fibroin heavy chain peptides or protein fragments, a plurality of fibroin light chain peptides or protein fragments, and / or a plurality of fibrohexamerin (p25) plurality of peptides or protein fragments, each peptide or protein fragment comprising a plurality of amino acids selected from M, R, V, K, T, F, I, L, C, A, Q, Y, N, D, E, G, S, H, P, and W, wherein at one or more independent occurrences at least one amino acid in a peptide or protein fragment is modified, substituted, or replaced.

2. The composition of claim 1, wherein a modification, substitution, and / or replacement is selected from an asparagine to aspartic acid modification, substitution, and / or replacement, a glutamine to glutamic acid modification, substitution, and / or replacement, and a methionine to methionine oxide modification, substitution, and / or replacement.

3. The composition of claim 1 or 2, wherein an amino acid modification, substitution, and / or replacement in a fibroin heavy chain peptide or protein fragment is at Q58, M64, N68, N70, N77, M80, N93, M103, Q125, N132, Q139, Q275, N4191, Q5216, and / or N5262, wherein each amino acid position is defined relative to the full sequence of the fibroin heavy chain.

4. The composition of claim 1 or 2, wherein an amino acid modification, substitution, and / or replacement in a fibroin light chain peptide or protein fragment is at N23, Q24, N28, M69, N105, N108, N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, and / or Q255, wherein each amino acid position is defined relative to the full sequence of the fibroin light chain.

5. The composition of claim 1 or 2, wherein an amino acid modification, substitution, and / or replacement in a fibrohexamerin (p25) chain peptide or protein fragment is at Q62, N93, M120, N149, N172, N174, and / or N202, wherein each amino acid position is defined relative to the full sequence of the fibrohexamerin (p25) chain. DB1 / 155183601.2 4836. The composition of any one of claims 1 to 5, the plurality of peptides or protein fragments having a weight average molecular weight (Mw) selected from between about 1 kDa and about 20 kDa, from between about 20 kDa and about 40 kDa, from between about 40 kDa and about 60 kDa, from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, from between about 100 kDa and about 120 kDa, from between about 120 kDa and about 140 kDa, from between about 140 kDa and about 160 kDa, from between about 160 kDa and about 180 kDa, from between about 180 kDa and about 200 kDa, from between about 200 kDa and about 250 kDa, or from between about 250 kDa and about 300 kDa.

7. The composition of claim 6, the plurality of peptides or protein fragments having a polydispersity between 1 and about 3.

8. The composition of any one of claims 1 to 5, the plurality of peptides or protein fragments having a weight average molecular weight (Mw) selected from between about 1 kDa and about 20 kDa, from between about 20 kDa and about 40 kDa, from between about 40 kDa and about 60 kDa, from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, from between about 100 kDa and about 120 kDa, from between about 120 kDa and about 140 kDa, or from between about 140 kDa and about 160 kDa, from between about 160 kDa and about 180 kDa, from between about 180 kDa and about 200 kDa, or from between about 200 kDa and about 250 kDa, and a polydispersity between 1 and about 1.7, or between about 1.7 and about 2.

5.

9. The composition of any one of claims 1 to 5, the plurality of peptides or protein fragments having a weight average molecular weight (Mw) selected from between about 10 kDa and about 20 kDa, from between about 20 kDa and about 40 kDa, from between about 40 kDa and about 60 kDa, from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, from between about 100 kDa and about 120 kDa, from between about 120 kDa and about 140 kDa, from between about 140 kDa and about 160 kDa, or from between about 160 kDa and about 180 kDa, and a polydispersity between 1 and about 1.1, between about 1.1 and about 1.2, or between about 1.2 and about 2.

4. DB1 / 155183601.2 48410. The composition of any one of claims 1 to 5, the plurality of peptides or protein fragments having a weight average molecular weight (Mw) selected from between about 40 kDa and about 60 kDa, from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, from between about 100 kDa and about 120 kDa, from between about 120 kDa and about 140 kDa, from between about 140 kDa and about 160 kDa, from between about 160 kDa and about 180 kDa, from between about 180 kDa and about 200 kDa, or from between about 200 kDa and about 220 kDa, and a polydispersity between 1 and about 1.7, or between about 1.7 and about 2.

5.

11. The composition of any one of claims 1 to 5, the composition comprising a plurality of peptides or protein fragments fractions.

12. The composition of claim 11, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 10 kDa and about 20 kDa, from between about 20 kDa and about 40 kDa, from between about 40 kDa and about 60 kDa, from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, from between about 100 kDa and about 120 kDa, or from between about 120 kDa and about 140 kDa, and a polydispersity between 1 and about 1.1, between about 1.1 and about 1.2, between about 1.2 and about 1.3, between about 1.3 and about 1.4, between about 1.4 and about 1.5, between about 1.5 and about 1.6, between about 1.6 and about 1.7, between about 1.7 and about 1.8, between about 1.8 and about 1.9, between about 1.9 and about 2, between about 2.1 and about 2.2, between about 2.2 and about 2.3, or between about 2.3 and about 2.

4.

13. The composition of claim 11, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, or from between about 100 kDa and about 120 kDa, and a polydispersity between 1 and about 1.1, between about 1.1 and about 1.2, between about 1.2 and about 1.3, between about 1.3 and about 1.4, between about 1.4 and about 1.5, between about 1.5 and about 1.6, between about 1.6 and about 1.7, between about 1.7 and about 1.8, between DB1 / 155183601.2 485about 1.8 and about 1.9, between about 1.9 and about 2, between about 2.1 and about 2.2, between about 2.2 and about 2.3, or between about 2.3 and about 2.

4.

14. The composition of claim 11, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 10 kDa and about 20 kDa, from between about 20 kDa and about 40 kDa, from between about 40 kDa and about 60 kDa, from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, or from between about 100 kDa and about 110 kDa, and a polydispersity between 1 and about 1.1, between about 1.1 and about 1.2, between about 1.2 and about 1.3, between about 1.3 and about 1.4, between about 1.4 and about 1.5, between about 1.5 and about 1.6, between about 1.6 and about 1.7, between about 1.7 and about 1.8, between about 1.8 and about 1.9, between about 1.9 and about 2, between about 2.1 and about 2.2, between about 2.2 and about 2.3, or between about 2.3 and about 2.

4.

15. The composition of claim 11, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, from between about 100 kDa and about 120 kDa, or from between about 120 kDa and about 140 kDa, and a polydispersity between 1 and about 1.1, between about 1.1 and about 1.2, between about 1.2 and about 1.3, between about 1.3 and about 1.4, between about 1.4 and about 1.5, between about 1.5 and about 1.6, between about 1.6 and about 1.7, between about 1.7 and about 1.8, between about 1.8 and about 1.9, between about 1.9 and about 2, between about 2.1 and about 2.2, between about 2.2 and about 2.3, or between about 2.3 and about 2.

4.

16. The composition of claim 11, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 20 kDa and about 40 kDa, or from between about 40 kDa and about 60 kDa, and a polydispersity between 1 and about 1.1, between about 1.1 and about 1.2, between about 1.2 and about 1.3, between about 1.3 and about 1.4, between about 1.4 and about 1.5, between about 1.5 and about 1.6, between about 1.6 and about 1.7, between about 1.7 and about 1.8, between about 1.8 and about 1.9, between about 1.9 DB1 / 155183601.2 486and about 2, between about 2.1 and about 2.2, between about 2.2 and about 2.3, or between about 2.3 and about 2.

4.

17. The composition of claim 11, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 40 kDa and about 60 kDa, from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, from between about 100 kDa and about 120 kDa, from between about 120 kDa and about 140 kDa, from between about 140 kDa and about 160 kDa, from between about 160 kDa and about 180 kDa, from between about 180 kDa and about 200 kDa, or from between about 200 kDa and about 210 kDa, and a polydispersity between 1 and about 1.1, between about 1.1 and about 1.2, between about 1.2 and about 1.3, between about 1.3 and about 1.4, between about 1.4 and about 1.5, between about 1.5 and about 1.6, between about 1.6 and about 1.7, between about 1.7 and about 1.8, between about 1.8 and about 1.9, between about 1.9 and about 2, between about 2.1 and about 2.2, between about 2.2 and about 2.3, or between about 2.3 and about 2.

4.

18. The composition of claim 11, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 40 kDa and about 60 kDa, from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, or from between about 100 kDa and about 110 kDa, and a polydispersity between 1 and about 1.1, between about 1.1 and about 1.2, between about 1.2 and about 1.3, between about 1.3 and about 1.4, between about 1.4 and about 1.5, between about 1.5 and about 1.6, between about 1.6 and about 1.7, between about 1.7 and about 1.8, between about 1.8 and about 1.9, between about 1.9 and about 2, between about 2.1 and about 2.2, between about 2.2 and about 2.3, or between about 2.3 and about 2.

4.

19. The composition of claim 11, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, from between about 100 kDa and about 120 kDa, from between about 120 kDa and about 140 kDa, from between about 140 kDa and about 160 kDa, from between about 160 kDa and about 180 kDa, from between about 180 kDa and about 200 kDa, or DB1 / 155183601.2 487from between about 200 kDa and about 210 kDa, and a polydispersity between 1 and about 1.1, between about 1.1 and about 1.2, between about 1.2 and about 1.3, between about 1.3 and about 1.4, between about 1.4 and about 1.5, between about 1.5 and about 1.6, between about 1.6 and about 1.7, between about 1.7 and about 1.8, between about 1.8 and about 1.9, between about 1.9 and about 2, between about 2.1 and about 2.2, between about 2.2 and about 2.3, or between about 2.3 and about 2.

4.

20. The composition of any one of claims 1 to 5, the plurality of peptides or protein fragments having a weight average molecular weight (Mw) selected from between about 10 kDa and about 30 kDa, from between about 30 kDa and about 50 kDa, or from between about 50 kDa and about 70 kDa, and a polydispersity between 1 and about 1.1, between about 1.1 and about 1.2, between about 1.2 and about 1.3, between about 1.3 and about 1.4, between about 1.4 and about 1.5, between about 1.5 and about 1.6, between about 1.6 and about 1.7, between about 1.7 and about 1.8, between about 1.8 and about 1.9, between about 1.9 and about 2, between about 2.1 and about 2.2, between about 2.2 and about 2.3, or between about 2.3 and about 2.

4.

21. The composition of claim 20, the plurality of peptides or protein fragments comprising one or more amino acid weight percentages selected from: amino acid %(w / w) STDEV%(w / w) 35567725428DB1 / 155183601.2 488methionine sulfone0.13945 ± 0.04579223(analytical amount equivalent to balance of thi i d thi i id i th l lit f 68599082, peptides or protein fragments fractions.

23. The composition of claim 22, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 1 kDa and about 20 kDa, from between about 20 kDa and about 40 kDa, or from between about 40 kDa and about 60 kDa, and a polydispersity between 1 and about 1.1, between about 1.1 and about 1.2, between about 1.2 and about 1.3, between about 1.3 and about 1.4, between about 1.4 and about 1.5, between about 1.5 and about 1.6, between about 1.6 and about 1.7, between about 1.7 and about 1.8, between about 1.8 and about 1.9, between about 1.9 and about 2, between about 2.1 and about 2.2, between about 2.2 and about 2.3, or between about 2.3 and about 2.4, wherein the peptides or protein fragments in the fraction are substantially neutral or positively charged.

24. The composition of claim 23, the plurality of peptides or protein fragments comprising one or more amino acid weight percentages selected from: amino acid %(w / w) STDEV61703DB1 / 155183601.2 489(analytical amount equivalent to glutamine and glutamic acid in the plurality of peptides or protein fragments) lin 415376 ± 100341711920062722052. p , p y p p p g nts in a fraction having a weight average molecular weight (Mw) selected from between about 20 kDa and about 40 kDa, from between about 40 kDa and about 60 kDa, or from between about 60 kDa and about 80 kDa, and a polydispersity between 1 and about 1.1, between about 1.1 and about 1.2, between about 1.2 and about 1.3, between about 1.3 and about 1.4, between about 1.4 and about 1.5, between about 1.5 and about 1.6, between about 1.6 and about 1.7, between about 1.7 and about 1.8, between about 1.8 and about 1.9, between about 1.9 and about 2, between about 2.1 and about 2.2, between about 2.2 and about 2.3, or between about 2.3 and about 2.4, wherein the peptides or protein fragments in the fraction are substantially negatively charged.

26. The composition of claim 25, the plurality of peptides or protein fragments comprising one or more amino acid weight percentages selected from: amino acid %(w / w) STDEV %(w / w)l i 24 2 2714 515836DB1 / 155183601.2 490(analytical amount equivalent to glutamine and glutamic acid in the plurality of peptides or protein f t 321659799697211. e compos on o any one o c a ms o , e p ura y o pep es or protein fragments having a weight average molecular weight (Mw) selected from between about 30 kDa and about 50 kDa, from between about 50 kDa and about 70 kDa, or from between about 70 kDa and about 90 kDa, and a polydispersity between 1 and about 1.1, between about 1.1 and about 1.2, between about 1.2 and about 1.3, between about 1.3 and about 1.4, between about 1.4 and about 1.5, between about 1.5 and about 1.6, between about 1.6 and about 1.7, between about 1.7 and about 1.8, between about 1.8 and about 1.9, between about 1.9 and about 2, between about 2.1 and about 2.2, between about 2.2 and about 2.3, or between about 2.3 and about 2.

4.

27. The composition of claim 26, the plurality of peptides or protein fragments comprising one or more amino acid weight percentages selected from: amino acid %(w / w) STDEV43527DB1 / 155183601.2 491(analytical amount equivalent to glutamine and glutamic acid in the plurality of peptides or protein fragments) lin 341335 ± 25984372153386378554. p , p p g p y peptides or protein fragments fractions.

29. The composition of claim 28, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 5 kDa and about 25 kDa, from between about 25 kDa and about 45 kDa, or from between about 45 kDa and about 65 kDa, and a polydispersity between 1 and about 1.1, between about 1.1 and about 1.2, between about 1.2 and about 1.3, between about 1.3 and about 1.4, between about 1.4 and about 1.5, between about 1.5 and about 1.6, between about 1.6 and about 1.7, between about 1.7 and about 1.8, between about 1.8 and about 1.9, between about 1.9 and about 2, between about 2.1 and about 2.2, between about 2.2 and about 2.3, or between about 2.3 and about 2.4, wherein the peptides or protein fragments in the fraction are substantially neutral or positively charged.

30. The composition of claim 29, the plurality of peptides or protein fragments comprising one or more amino acid weight percentages selected from: amino acid %(w / w) STDEV814DB1 / 155183601.2 492amino acid %(w / w) STDEV%(w / w) l ti l t i l t t i ti 0941994003721901331. The composition of claim 28, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 35 kDa and about 55 kDa, from between about 55 kDa and about 75 kDa, or from between about 75 kDa and about 95 kDa, and a polydispersity between 1 and about 1.1, between about 1.1 and about 1.2, between about 1.2 and about 1.3, between about 1.3 and about 1.4, between about 1.4 and about 1.5, between about 1.5 and about 1.6, between about 1.6 and about 1.7, between about 1.7 and about 1.8, between about 1.8 and about 1.9, between about 1.9 and about 2, between about 2.1 and about 2.2, between about 2.2 and about 2.3, or between about 2.3 and about 2.4, wherein the peptides or protein fragments in the fraction are substantially negatively charged.

32. The composition of claim 31, the plurality of peptides or protein fragments comprising one or more amino acid weight percentages selected from: amino acid %(w / w) STDEV452DB1 / 155183601.2 493(analytical amount equivalent to asparagine and aspartic acid in the plurality of peptides or protein fragments) tin di lfid 000752 ± 0013024561260219153069. e compos on o any one o c a ms o , w ere n a pep e or pro ein fragment comprises between one and ten modifications, substitutions, and / or replacements.

34. The composition of any one of claims 1 to 33, wherein each modification, substitution, and / or replacement is independently ranging in the composition between about 1% to 100%.

35. The composition of any one of claims 1 to 34, wherein % modification, substitution, and / or replacement is defined as (number of peptide or protein fragments comprising a modification, substitution, and / or replacement at a specific position, divided by the total number of peptide or protein fragments which include the specific position, whether comprising a modification, substitution, and / or replacement, or not) x 100.

36. The composition of any one of claims 1 to 35, wherein a molecular weight is determined by MALS. DB1 / 155183601.2 49437. The composition of any one of claims 1 to 36, wherein a peptide or protein fragment comprises an unnatural amino acid sequence when compared to any sequence comprised in a natural fibroin heavy chain, natural fibroin light chain, and / or natural fibrohexamerin (p25).

38. The composition of any one of claims 1 to 36, comprising one or more crosslinks between two or more peptides or protein fragments selected.

39. An article comprising the composition of any one of claims 1 to 38.

40. A method for determining a characteristic of a composition of any one of claims 1 to 38, the method comprising: determine a profile for protein staining of the composition across a range of temperatures, and compare the profile or a portion thereof to a reference, wherein the composition comprises a solvent phase comprising at least one alcohol.

41. The method of claim 40, wherein the alcohol is 2-propanol.

42. The method of claim 40 or 41, wherein the profile for protein staining comprises a profile of normalized rate of SYPRO binding. DB1 / 155183601.2 495

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