Auto-coding electrode structure strip using auto-code method, electrode structure thereof, and manufacturing method therefor

Abstract

The present invention relates to an auto-coding electrode structure strip using an auto-code method for recognizing a code through a combination of differences between electrodes, an electrode structure thereof, and a manufacturing method therefor and, more specifically, to an auto-coding electrode structure strip formed by stacking a first substrate, a second substrate, and a third substrate, followed by selective punching at a designated position, and then stacking a fourth substrate, an electrode structure thereof, and a manufacturing method therefor.

Description

Autocoding electrode structure strip using auto code method, electrode structure thereof and manufacturing method thereof

[0001] The present invention relates to an autocoding electrode structure strip using an auto code method, an electrode structure thereof, and a method for manufacturing the same, and more particularly, to an autocoding electrode structure strip using an auto code method that recognizes a code by a combination of differences between electrodes, an electrode structure thereof, and a method for manufacturing the same.

[0002] Quantitatively or qualitatively analyzing analytes present in biological samples is a crucial task both chemically and clinically. Representative examples include measuring blood sugar levels in diabetic patients and cholesterol, a risk factor for various adult diseases.

[0003] As is known in the field, in electrochemical biosensors utilizing enzyme activity, it is very important to measure enzyme activity such as specific substances in biological samples (hereinafter referred to as "specimens"), for example, saliva or blood, glucose, uric acid, protein, DNA, sucrose in clinical chemistry tests, and GOT (Glutamate-Oxaloacetate Transaminase) or GPT (Glutamate-Pyruvate Transaminase) in liver function tests, more quickly and reproducibly. Here, the biosensor is divided into an identification part that identifies the measurement target and a conversion part that converts it into an electrical signal.

[0004] The identification site uses a biological material. When the biological material recognizes the target, a chemical or physical change occurs. The part that converts this change into an electrical signal is the transformation site. The identification site and the transformation site are collectively called the biosensor electrode.

[0005] Currently commercialized strip-type biosensors have manufacturing process deviations due to problems with the raw materials themselves, seasonal variations, or temperature / humidity. To compensate for these process deviations, the manufacturing characteristics of each manufactured strip must be recognized, and the recognized characteristics of the strip must be utilized to compensate for the process deviation. Depending on the method of recognizing the correction code of the strip, there are no-code (or one-code), manual code, auto-code, and chip-code methods. Except for the no-code (or one-code) method, which requires a lot of capital, time, and a high level of effort, the most widely used conventional auto-code method inputs the code through a printing process in the final process after producing the strip, and recognizes it as a pattern. However, due to the complexity of the equipment, there was a limitation in that it affected the sensor.

[0006] The purpose of the present invention is to recognize a strip code by a combination of differences between electrodes using an auto code method.

[0007] The purpose of the present invention is to enable accurate code recognition by representing a code as a combination of open circuits and short circuits within an electrode circuit through a punching operation after implementing an electrode circuit pattern.

[0008] However, the technical problems to be solved by the present invention are not limited to the above problems, and can be expanded in various ways without departing from the technical spirit and scope of the present invention.

[0009] In an autocoding electrode structure strip using an autocode method according to an embodiment of the present invention, after a first substrate, a second substrate, and a third substrate are laminated, a fourth substrate is laminated and formed after a selective punching operation at a designated location.

[0010] A method for manufacturing an autocoding electrode structure strip using an auto code method according to an embodiment of the present invention comprises the steps of forming a first substrate, forming a second substrate on top of the first substrate, forming a third substrate on top of the second substrate, performing a selective punching operation at a designated coding punching position on the first substrate in a code punching area within the third substrate, and forming a fourth substrate on top of the third substrate after the punching operation.

[0011] In the electrode structure of the autocoding electrode structure strip using the auto code method according to an embodiment of the present invention, the strip recognition electrode used to recognize that the autocoding electrode structure strip is inserted into a connector which is a sample measuring device, a sample recognition electrode used to recognize a sample, and a coding electrode used to recognize an open circuit and a short circuit within the electrode circuit due to a selective punching operation are included.

[0012] According to an embodiment of the present invention, after implementing an electrode circuit pattern, an auto-code method is used to represent a code as a combination of open and short circuits within the electrode circuit through a punching operation, thereby enabling more accurate strip code recognition based on the differential combination between electrodes. Furthermore, compared to conventional methods of recognizing codes through printing, the present invention enables a simple manufacturing process without affecting sensor performance.

[0013] According to an embodiment of the present invention, by laminating the first to third substrates and then laminating the fourth substrate after a selective punching operation at a designated location to form an autocoding electrode structure strip, the accuracy and stability of the autocode method can be increased, and the technical effect of improving the strip recognition accuracy can be achieved.

[0014] However, the effects of the present invention are not limited to the above effects, and can be expanded in various ways without departing from the technical spirit and scope of the present invention.

[0015] FIG. 1 is a structural diagram of an autocoding electrode structure strip using an autocode method according to an embodiment of the present invention.

[0016] Figure 2 illustrates an example of inserting the combination diagram of the autocoding electrode structure strip described in Figure 1 into a connector.

[0017] FIG. 3a and FIG. 3b illustrate an electrode structure of a first substrate of an autocoding electrode structure strip according to an embodiment of the present invention.

[0018] FIGS. 4A and 4B illustrate drawings of a first substrate of an autocoding electrode structure strip according to an embodiment of the present invention.

[0019] FIGS. 5A to 5C illustrate drawings in which a coding punching position of a first substrate of an autocoding electrode structure strip according to an embodiment of the present invention is formed.

[0020] FIG. 6 illustrates a flow chart of a method for manufacturing an autocoding electrode structure strip according to an embodiment of the present invention.

[0021] The advantages and features of the present invention, and the methods for achieving them, will become clearer with reference to the embodiments described in detail below together with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below, but may be implemented in various different forms. These embodiments are provided solely to ensure that the disclosure of the present invention is complete and to fully inform those skilled in the art of the scope of the invention, and the present invention is defined solely by the scope of the claims.

[0022] The terminology used herein is for the purpose of describing embodiments and is not intended to limit the present invention. In this specification, the singular also includes the plural unless specifically stated otherwise. As used herein, the terms "comprises" and / or "comprising" do not exclude the presence or addition of one or more other components, steps, operations, and / or elements.

[0023] Unless otherwise defined, all terms (including technical and scientific terms) used herein may be used in their common sense to those of ordinary skill in the art to which the present invention pertains. Furthermore, terms defined in commonly used dictionaries are not to be interpreted ideally or excessively unless explicitly and specifically defined otherwise.

[0024] Hereinafter, preferred embodiments of the present invention will be described in more detail with reference to the attached drawings. Identical components in the drawings are designated by the same reference numerals, and redundant descriptions of identical components are omitted.

[0025]

[0026] The present invention proposes an autocoding electrode structure strip using an auto code method that recognizes a code by a combination of differences between electrodes, an electrode structure thereof, and a method for manufacturing the same.

[0027] The present invention can provide high-accuracy strip code recognition with a simple manufacturing process by using an auto code method that represents a combination of open circuits and short circuits within an electrode circuit as a code through a punching operation after implementing an electrode circuit pattern.

[0028] Hereinafter, the present invention will be described in detail with reference to FIGS. 1 to 6.

[0029]

[0030] FIG. 1 illustrates a structural diagram of an autocoding electrode structure strip using an autocode method according to an embodiment of the present invention. FIG. 2 also illustrates an example of inserting the coupling diagram of the autocoding electrode structure strip described in FIG. 1 into a connector. FIG. 3a and FIG. 3b illustrate the electrode structure of the first substrate of the autocoding electrode structure strip according to an embodiment of the present invention.

[0031] Referring to FIG. 1, an autocoding electrode structure strip (100) using an auto code method according to an embodiment of the present invention is formed in such a way that a first substrate (110), a second substrate (120), and a third substrate (130) are laminated, and then a fourth substrate (140) is laminated after a selective punching operation is performed at a designated location in the first substrate (110). Here, the punching operation means making a hole at a designated location in the first substrate (110), and the present invention proposes a punching method. However, the present invention is not limited to the punching method, and any method may be used as long as it provides a connection or disconnection of a conductor by punching through designated locations on the front and back surfaces of the first substrate (110).

[0032] The autocoding electrode structure strip (100) performs the function of sensing information to be measured from a sample from which interference substances have been removed when a collected sample is contacted and inserted. In the present invention, a code is inserted into the autocoding electrode structure strip (100) by a punching operation in an auto code manner, and the code can be recognized by a combination of differences between the electrodes of the autocoding electrode structure strip (100) through a connector (200) (see FIG. 2).

[0033] An autocoding electrode structure strip (100) according to an embodiment of the present invention may be composed of a first substrate (110), a second substrate (120), a third substrate (130), and a fourth substrate (140). In more detail, the autocoding electrode structure strip (100) includes, as shown in FIG. 1, a first substrate (110) in which an electrode structure and a code forming area (111) in which a selective punching operation is performed are formed, a second substrate (120) including a sample insertion path (121) in which an enzyme compound (123) that reacts with a sample is inserted while being placed on the first substrate (110) and a first code punching area (122) for a punching operation on the first substrate (110), a third substrate (130) including an insertion port (131) for inserting a sample into the sample insertion path (121) while being placed on the second substrate (120) and an exhaust port (132) for discharging air, and a second code punching area (133) for a punching operation on the first substrate (110), and a third substrate (130) disposed on the third substrate (130) to protect the coding punching formed on the first substrate (110). It may include a fourth substrate (140) which is a cover.

[0034] At this time, the autocoding electrode structure strip (100) according to the embodiment of the present invention is characterized in that after the first substrate (110), the second substrate (120), and the third substrate (130) are laminated, a selective punching operation is performed in the code forming area (111), which is a designated location of the first substrate (110), through the second code punching area (133) of the third substrate (130) and the first code punching area (122) of the second substrate (120), and after the punching operation, the fourth substrate (140) is laminated. The first substrate (110) may have a carbon electrode formed thereon, the second substrate (120) may be formed with a double-sided adhesive, the third substrate (130) may be formed with hydrophilic PET, and the fourth substrate (140) may be formed with a single adhesive PET.

[0035] Here, the sample insertion path (121) is self-evident to those skilled in the art, so a detailed description thereof is omitted.

[0036] The enzyme compound (123) may include an enzyme for selectively reacting glucose contained in a sample, a polymer for attaching the enzyme to electrodes arranged on the lower plate (130), and a catalyst for promoting the reaction between the enzyme and glucose.

[0037] At this time, the catalyst may contain 1 to 10% of at least one of ferrocene, a ferrocene derivative, quinone, a quinone derivative, hexaamineruthenium(III) chloride, Prussian blue, and ferricyanide (when Prussian blue is used, the catalyst may contain 0.001 to 5% of Prussian blue), the enzyme may contain 0.01 to 1.0 wt% of at least one of glucose oxidase and glucose dehydrogenase (GDH), and the polymer may contain 1 to 30 units of at least one of Chitosan, PVP, Nafion, polyethylene glycol, poly vinyl Pyrrolidone, poly vinyl alcohol, agarose, and trehalose.

[0038] An electrode structure of a carbon electrode including a strip recognition electrode (301), a sample recognition electrode (302), a coding electrode (303, 304), a sample recognition / strip recognition reference electrode (305), a working electrode (306), and a working reference electrode (307) may be formed on the front surface of the first substrate (110) (see FIG. 3a). In addition, an electrode structure of a carbon electrode including a coding reference electrode (311) and coding electrodes (312 to 317) may be formed on the back surface of the first substrate (110) (see FIG. 3b).

[0039] The strip recognition electrode (301) can be used to recognize whether an autocoding electrode structure strip (100) is inserted into a connector (200), which is a sample measuring device. For example, a strip recognition signal is applied from the connector (200) through the strip recognition electrode (301) and the recognition reference electrode (305), and a response signal in response thereto can be provided from the autocoding electrode structure strip (100) to the connector (200) through the strip recognition electrode (301) and the recognition reference electrode (305). Accordingly, the connector (200) can recognize whether an autocoding electrode structure strip (100) is inserted into the connector (200) using the strip recognition electrode (301). Whether an autocoding electrode structure strip (100) is inserted into the connector (200) can be determined based on the intensity of a response signal in response to the strip recognition signal or whether a response signal is generated and provided.

[0040] The specimen recognition electrode (302) can be used to recognize a specimen. More specifically, the specimen recognition electrode (302) can be used to recognize whether a specimen has come into contact with the autocoding electrode structure strip (100). For example, a specimen recognition signal is applied from a connector (200) or a specimen measuring device (not shown) through the specimen recognition electrode (302) and the recognition reference electrode (305), and a response signal in response thereto can be provided from the autocoding electrode structure strip (100) to the connector (200) or the specimen measuring device (not shown) through the specimen recognition electrode (302) and the recognition reference electrode (305). Therefore, the connector (200) or the specimen measuring device (not shown) can recognize whether a specimen has come into contact with the autocoding electrode structure strip (100) using the specimen recognition electrode (302). Whether a sample is in contact with the autocoding electrode structure strip (100) can be determined based on the intensity of the response signal in response to the sample recognition signal or the presence or absence of the response signal being generated and provided.

[0041] At this time, the specimen recognition / strip recognition reference electrode (305) for each of the strip recognition electrode (301) and the specimen recognition electrode (302) may be provided in common for the strip recognition electrode (301) and the specimen recognition electrode (302). That is, the strip recognition electrode (301) and the specimen recognition electrode (302) may share one specimen recognition / strip recognition reference electrode (305). Accordingly, unlike the case where the strip recognition electrode (301) and the specimen recognition electrode (302) each have their own recognition reference electrode, miniaturization of the autocoding electrode structure strip (100) may be possible.

[0042] The working electrode (306) and the working reference electrode (307) can be used to measure a sample. Specifically, a sample measurement signal is applied from the connector (200) or a sample measurement device (not shown) through the working electrode (306) and the working reference electrode (307), and a response signal for the sample in response thereto can be provided from the autocoding electrode structure strip (100) to the connector (200) or the sample measurement device (not shown) through the working electrode (306) and the working reference electrode (307). Accordingly, the connector (200) or the sample measurement device (not shown) can measure the sample by measuring the response signal for the sample using the working electrode (306) and the working reference electrode (307). The sample measurement result can be determined based on the intensity of the response signal in response to the sample measurement signal or the presence or absence of generation and provision of the response signal. For example, the sample measurement result can indicate several levels of measurement values ​​depending on the intensity of the response signal in response to the sample measurement signal.

[0043] The coding electrodes (303, 304, and 312 to 317) and the coding reference electrode (311) can be used to detect open circuits and short circuits within the electrode circuit due to selective punching operations. The coding electrodes formed on the front surface of the first substrate (110) (see FIG. 3a) can be composed of a first coding electrode (303) and a second coding electrode (304), and the coding electrodes formed on the back surface of the first substrate (110) (see FIG. 3b) can be composed of a third coding electrode (312), a fourth coding electrode (313), a fifth coding electrode (314), a sixth coding electrode (315), a seventh coding electrode (316), and an eighth coding electrode (318). This means that the code forming area (111) of the designated position where the selective punching operation is performed within the first substrate (110) includes eight coding punching positions in two rows and four columns as shown in FIG. 3a. Accordingly, the first to eighth coding electrodes (303, 304 and 312 to 317) can be used to detect open circuits and short circuits according to changes in resistance values ​​at each of the eight coding trigger positions.

[0044] A punching operation is formed by punching at least two of eight coding punching positions, and the first to eighth coding electrodes (303, 304 and 312 to 317) can recognize a code by a combination of differences between electrodes according to open circuits and short circuits at the coding punching positions using an auto code method. More specifically, the first to eighth coding electrodes (303, 304 and 312 to 317) can detect a short circuit having zero resistance at a punched coding punching position in an electrode structure connected to each of the eight coding punching positions, can detect an open circuit having unlimited resistance at a non-punched coding punching position, and can encode a combination of differences between electrodes according to open circuits and short circuits into binary. For example, the first to eighth coding electrodes (303, 304 and 312 to 317) can be selectively punched to code the autocoding electrode structure strip (100) in binary by specifying '0' when detected as a short circuit and '1' when detected as an open circuit at each of the eight coding punching positions. At this time, since the punching operation is formed by penetrating the first substrate (110), the same punching result is obtained not only on the front side but also on the back side.

[0045] The strip recognition electrode (301), the sample recognition electrode (302), the first to eighth coding electrodes (303, 304 and 312 to 317), the sample recognition / strip recognition reference electrode (305), the working electrode (306) and the working reference electrode (307) of the autocoding electrode structure strip (100) described above can be formed to extend in one direction while being spaced apart from each other in parallel.

[0046] In the electrode structure described above, the strip recognition electrode (301) and the sample recognition electrode (302) can be provided separately so as to electrically operate independently from the working electrode (306) and the working reference electrode (307). Therefore, the accuracy of sample measurement can be improved by preventing an electric double layer from being formed on the surfaces of the working electrode (306) and the working reference electrode (307) prior to sample measurement.

[0047] The air electrode (308) is intended to improve the poor dispersion of the interfacial material properties by allowing the sample to contact the sample insertion path (121) over a larger area than the preset size, and may be provided on one side of the area corresponding to the sample insertion path (121) in the first substrate (110).

[0048] Although not illustrated in a separate drawing, the first substrate (110) on which the described electrode structure is formed may be formed by overlapping a plurality of metal layers. For example, the first substrate (110) may be implemented as a four-layer structure in which an insulating substrate layer (not illustrated), a first metal layer (not illustrated) formed of copper on the insulating substrate layer, a second metal layer (not illustrated) formed of nickel on the first metal layer, and a third metal layer (not illustrated) formed of gold on the second metal layer are overlapping. In this case, the above-described electrode structure may be formed on each of the first metal layer, the second metal layer, the third metal layer, and the fourth metal layer.

[0049] As the biosensor strip (120) with the sample in contact and introduced is inserted, the connector (200) or the sample measuring device (not shown) sequentially applies a strip recognition signal, a sample recognition signal, and a sample measurement signal to the autocoding electrode structure strip (100), and receives a response signal for each of the strip recognition signal, the sample recognition signal, and the sample measurement signal, thereby recognizing that the autocoding electrode structure strip (100) has been inserted and that the sample has been in contact with the autocoding electrode structure strip (100), and can measure the sample.

[0050] The result of measuring a sample can be provided as a measurement value in several stages according to the strength of a response signal responding to a sample measurement signal, and different graphic effects can be applied according to the measurement value and displayed through a display means of a sample measurement device (not shown).

[0051]

[0052] FIGS. 4A and 4B illustrate drawings of a first substrate of an autocoding electrode structure strip according to an embodiment of the present invention, and FIGS. 5A to 5C illustrate drawings in which a coding punching position of a first substrate of an autocoding electrode structure strip according to an embodiment of the present invention is formed.

[0053] Fig. 4a illustrates the front side of the first substrate having an electrode structure, and Fig. 4b illustrates the back side of the first substrate having an electrode structure. In Figs. 4a and 4b, no punching positions for coding are formed.

[0054] Fig. 5a illustrates the front side of the first substrate to which the electrode structure and the punching position for coding are applied, Fig. 5b illustrates the back side of the first substrate to which the electrode structure and the punching position for coding are applied, and Fig. 5c illustrates the punching position for coding.

[0055] When the punching operation according to the embodiment of the present invention is applied, a drawing of the first substrate punched equally on the front and back sides can be confirmed as shown in FIGS. 5a to 5c.

[0056] In the autocoding electrode structure strip (100) according to the embodiment of the present invention illustrated in FIG. 1, a code forming area (111) at a designated location where a selective punching operation is performed within the first substrate (110) represents a space where at least one coding punching position (112) is formed. In the present invention, it is described that eight coding punching positions (112) are provided to provide an 8-bit code, but the number is not limited.

[0057] As shown in FIGS. 5a and 5b, the coding punching positions (112) are located within the code forming area (111), and include eight coding punching positions (112) in four rows and two columns based on FIGS. 5a and 5b. Each of the eight coding punching positions (112) is formed on each of the first to eighth coding electrodes arranged on the first substrate (110), so that when selective punching (113) is applied among the coding punching positions (112), the corresponding coding electrode can detect open circuits and short circuits according to connection and disconnection, and recognize the code of the strip (100) by a combination thereof (see FIG. 5c). At this time, in FIG. 5c, the coding punching positions (112) may have a square shape with an area of ​​1.5 mm × 1.5 mm, and the diameter of the punching (113) may be about 1.0 to 1.2 mm.

[0058]

[0059] Fig. 6 illustrates a flow chart of a method for manufacturing an autocoding electrode structure strip according to an embodiment of the present invention. The manufacturing method described below is based on the premise that the device for manufacturing the autocoding electrode structure described above with reference to Figs. 1 to 5 is used as the main body.

[0060] Referring to FIG. 6, in step S610, a first substrate is formed. The first substrate is formed with an electrode structure and a code formation area where an optional punching operation is performed. At this time, a carbon electrode may be formed on the first substrate.

[0061] In step S620, a second substrate is formed on top of the first substrate. The second substrate includes a specimen insertion path into which an enzyme compound that reacts with a specimen is inserted while positioned on top of the first substrate, and a first code punching area for punching on the first substrate. At this time, the second substrate may be formed using a double-sided adhesive.

[0062] In step S630, a third substrate is formed on top of the second substrate. The third substrate is positioned on top of the second substrate and includes an insertion port for inserting a sample into a sample insertion path, an exhaust port for exhausting air, and a second code punching area for punching the first substrate. At this time, the third substrate may be formed of hydrophilic PET.

[0063] In step S640, a selective punching operation is performed at a coding punching position designated in the first substrate in a code punching area in the third substrate. In step S640, a punching operation is performed to punch at least two of the eight coding punching positions, and the first to eighth coding electrodes in the first substrate by the punching formed in the first substrate can recognize a code by using an auto code method as a combination of differences between electrodes according to open circuits and short circuits at the coding punching positions.

[0064] In step S650, a fourth substrate is formed on top of the third substrate after the punching operation. The fourth substrate may be placed on top of the third substrate after the punching operation in step S640 and may serve as a cover to protect the coding punching formed on the first substrate. At this time, the fourth substrate may be formed of a single adhesive PET.

[0065]

[0066] Although the embodiments described above have been described by way of limited examples and drawings, those skilled in the art will appreciate that various modifications and variations can be made based on the above teachings. For example, appropriate results can still be achieved even if the described techniques are performed in a different order than described, and / or components of the described systems, structures, devices, circuits, etc. are combined or combined in a different manner than described, or are replaced or substituted with other components or equivalents.

[0067]

[0068] Therefore, other implementations, other embodiments, and equivalents to the claims also fall within the scope of the claims described below.

Claims

1. In a biosensor using the auto code method, A first substrate comprising a plurality of electrodes and a code forming area for identifying the biosensor; A second substrate disposed on the first substrate and including a first opening corresponding to a sample insertion path and the code forming area; and A third substrate disposed on the second substrate and including a specimen insertion port, an air exhaust port, and a second opening corresponding to the code forming area; including, A biosensor using the auto-code method.

2. In paragraph 1, A fourth substrate disposed on the third substrate and covering the code forming area, the first opening, and the second opening; including more, A biosensor using the auto-code method.

3. In paragraph 1, The above code forming area includes at least one punching position for performing a selective punching operation. A biosensor using the auto-code method.

4. In paragraph 3, The electrode comprises at least one coding electrode, At least one portion of the above coding electrode is located at the punching position, A biosensor using the auto-code method.

5. In paragraph 4, The electrode further comprises at least one reference electrode, The above coding electrode forms a predetermined circuit with the reference electrode, The above circuit, If the part of the coding electrode located at the above firing position is disconnected, it is a short circuit, If the part of the coding electrode located at the above firing position is not disconnected, it is an open circuit. A biosensor using the auto-code method.

6. In paragraph 5, The combination of the above short circuit and the above open circuit corresponds to the code on the code forming area, The above coding electrode is for identifying the biosensor based on the code. A biosensor using the auto-code method.

7. In paragraph 5, The above coding electrodes are located, some on the front side of the first substrate, and the remaining parts on the back side of the first substrate. The part of the above coding electrode constitutes the circuit with the reference electrode located on the front side of the first substrate, The remaining part of the above coding electrode is located on the back side of the first substrate among the above reference electrodes and constitutes the circuit. A biosensor using the auto-code method.

8. In paragraph 3, The above punching position includes a square shape and includes a punching area inside the square. A biosensor using the auto-code method.

9. In a method for manufacturing a biosensor using an auto code method, A step of forming a first substrate including a plurality of electrodes and a code forming area for identifying the biosensor; A step of forming a second substrate including a specimen insertion path and a first opening on the first substrate; A step of forming a third substrate including a specimen insertion port, an air exhaust port, and a second opening on the second substrate; and A step of performing a selective punching operation on the code forming area by penetrating the first opening and the second opening; including, A method for manufacturing a biosensor using an auto code method.

10. In paragraph 9, A step of forming a fourth substrate on top of the third substrate, which covers the code forming area, the first opening, and the second opening; including more, A method for manufacturing a biosensor using an auto code method.

11. In a biosensor for detecting glucose in a sample, A first substrate comprising a working electrode and a reference electrode for detecting the glucose, wherein the working electrode comprises at least one working electrode protrusion, and the reference electrode comprises at least one reference electrode protrusion, a sample recognition electrode for recognizing the sample, wherein the sample recognition electrode comprises at least one sample recognition electrode protrusion, and at least one blank electrode; A second substrate disposed on the first substrate and including a specimen insertion path in which a reagent layer is disposed; and A third substrate disposed on the second substrate and including a specimen insertion port and an air exhaust port; Including, The first substrate further includes a code forming region for identifying the biosensor, At least a portion of each of the working electrode protrusion, the reference electrode protrusion, the sample recognition electrode protrusion, the idle electrode, and the first substrate constitutes an area corresponding to the sample insertion path, The above-mentioned electrode is placed at the longitudinal end of the area corresponding to the sample insertion path. A biosensor for detecting glucose in a sample.

12. In paragraph 11, At least a portion of each of the working electrode protrusion, the reference electrode protrusion, the sample recognition electrode protrusion, the idle electrode, and the first substrate constitutes an area corresponding to the reagent layer. A biosensor for detecting glucose in a sample.

13. In paragraph 12, The area corresponding to the above reagent layer overlaps the area corresponding to the above specimen insertion path. A biosensor for detecting glucose in a sample.

14. In paragraph 12, The area corresponding to the above reagent layer corresponds to the area corresponding to the above specimen insertion path. A biosensor for detecting glucose in a sample.

15. In paragraph 11, The above reagent layer is in contact with an area corresponding to the sample insertion path by a size greater than a preset size due to the above-mentioned air electrode. A biosensor for detecting glucose in a sample.

16. In paragraph 11, The above-mentioned electrode is configured to improve the dispensing spreadability of the reagent layer. A biosensor for detecting glucose in a sample.

17. In paragraph 11, At least some of the above-mentioned electrodes are arranged in an area on the first substrate corresponding to the sample insertion port. A biosensor for detecting glucose in a sample.

18. In paragraph 11, At least some of the above-mentioned electrodes are arranged with the specimen recognition electrode protrusions interposed therebetween. A biosensor for detecting glucose in a sample.

19. In paragraph 11, The second substrate further includes a first opening corresponding to the code forming area, The third substrate further includes a second opening corresponding to the code forming area. A biosensor for detecting glucose in a sample.

20. In paragraph 19, A fourth substrate disposed on the third substrate and covering the code forming area, the first opening, and the second opening; including more, A biosensor for detecting glucose in a sample.

21. In paragraph 19, The above code forming area includes at least one punching position for performing a selective punching operation. A biosensor for detecting glucose in a sample.

22. In paragraph 21, The electrode comprises at least one coding electrode, At least one portion of the above coding electrode is located at the punching position, A biosensor for detecting glucose in a sample.

23. In paragraph 22, The electrode further comprises at least one reference electrode, The above coding electrode forms a predetermined circuit with the reference electrode, The above circuit, If the part of the coding electrode located at the above firing position is disconnected, it is a short circuit, If the part of the coding electrode located at the above firing position is not disconnected, it is an open circuit. A biosensor for detecting glucose in a sample.

24. In paragraph 23, The combination of the above short circuit and the above open circuit corresponds to the code on the code forming area, The above coding electrode is for identifying the biosensor based on the code. A biosensor for detecting glucose in a sample.

25. In paragraph 23, The above coding electrodes are located, some on the front side of the first substrate, and the remaining parts on the back side of the first substrate. The above part of the above coding electrode forms a predetermined circuit with the reference electrode located on the front side of the first substrate, The remaining part of the above coding electrode forms a predetermined circuit with the reference electrode located on the rear side of the first substrate. A biosensor for detecting glucose in a sample.

26. In paragraph 21, The above punching position includes a square shape and includes a punching area inside the square. A biosensor for detecting glucose in a sample.

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