Preparation method for rosemary leaf extract rich in lactose matrine molecular machine

Abstract

A preparation method for a rosemary leaf extract rich in a lactose matrine molecular machine. The method uses a lactose matrine molecular machine-coupled microwave-assisted extraction process to enable efficient and environmentally friendly extraction of polyphenol compounds including rosmarinic acid from rosemary leaves. The ability of the lactose matrine molecular machine to selectively extract effective compounds is verified. A new rosemary leaf powder is added in the same solid-liquid ratio to verify the reusability of the lactose matrine molecular machine, maximizing the enrichment of polyphenol compounds including rosmarinic acid. Finally, the extract obtained by subjecting fresh rosemary leaves to repeated extraction processes is heated and passed through a spray dryer to form spherical particles of co-spray drying of active substances such as the lactose matrine molecular machine, plant cellulose, and polyphenol compounds. The spherical particles can stably exist and relieve inflammation, promote the proliferation and migration of hair papillary cells, regulate the periodic circulation of hair follicles, and promote hair growth.

Description

Preparation method of rosemary leaf extract rich in lactosmatrine molecular machinery Technical Field

[0001] This invention relates to a method for preparing a rosemary leaf extract rich in lactosmatrine molecular machinery, belonging to the field of plant active ingredient extraction technology. Background Technology

[0002] Rosemary is currently one of the most effective herbs for promoting hair growth. It is a typical plant used both as food and medicine, with rich applications in medicine, fried foods, and food preservation. Research indicates that these applications are primarily related to the phenols and volatile oils in rosemary's chemical composition. In addition to ursolic acid and α-pinene, rosemary extracts are rich in polyphenolic compounds, such as caryophyllene and diterpenoids like rosmarinic acid. Rosmarinic acid possesses free radical scavenging activity and antioxidant properties; it exhibits broad-spectrum antimicrobial activity, inhibiting both bacteria and fungi, such as Bacillus subtilis, Micrococcus luteus, Escherichia coli, Staphylococcus aureus, and F. rhizoctonia solani; it alleviates atopic dermatitis, also known as atopic eczema or hereditary allergic eczema; and it shows particular activity against type I and type II herpes simplex virus, making it an effective component for controlling herpes. Its antiviral activity, besides inhibiting certain enzyme activities in the viral life cycle, also rapidly binds to the viral capsid protein, thereby inactivating the virus. Therefore, research on extraction solvents and processes for rosmarinic polyphenols, including rosmarinic acid, is of significant value.

[0003] Molecular machines are composed of biomolecules at the molecular scale, such as proteins, amino acids, choline, and fruit acids. They are functional machines achieved through specific structural designs. Molecular machines are characterized by their small size, diversity, self-assembly, self-adaptation, wide polarity range, and high designability. In the extraction of plant bioactive substances, they can not only preserve the activity of the substances but also, through optimized design, achieve the dissolution of hemicellulose and the depolymerization of lignin, increasing the permeability of plant cell walls, improving the solubility of intracellular active components, enhancing the stability of bioactive substances, and promoting transdermal absorption. Matrine is a natural alkaloid derived from the fruit, root, and foliage of *Sophora flavescens*, possessing various effects including antiviral, anti-inflammatory, and antibacterial properties. Lactose is a carbohydrate unique to human and mammalian milk, a disaccharide composed of glucose and galactose, and has functions such as filling, binding, shaping, moisturizing, and inhibiting the growth of putrefactive bacteria.

[0004] Currently, the main extraction solvents used for rosemary leaf polyphenols are water and ethanol, or their blends. These methods suffer from poor selectivity, low solubility, low content of active ingredients, excessively long extraction times, large solvent consumption, and the need for complete removal of the ethanol solvent. The resulting products exhibit poor stability, easy decomposition of active ingredients, susceptibility to spoilage and mold growth, and difficulty in guaranteeing efficacy. Therefore, there is an urgent need to develop new, green, and efficient extraction solvents to effectively solve these problems.

[0005] In view of the above-mentioned shortcomings, the present invention aims to create a method for preparing rosemary leaf extract rich in lactosmatrine molecular machinery, so as to make it more industrially valuable. Summary of the Invention

[0006] To address the aforementioned technical problems, the present invention aims to provide a method for preparing a rosemary leaf extract rich in lactometramine molecular machinery. This method utilizes a microwave-assisted extraction process coupled with lactometramine molecular machinery to achieve a highly efficient and green 8-minute extraction of polyphenolic compounds, including rosmarinic acid, from rosemary leaves. The filtrate is collected, and fresh rosemary leaf powder is added at the same solid-liquid ratio, verifying the reusability of the lactometramine molecular machinery and maximizing the enrichment of polyphenolic compounds, including rosmarinic acid. Subsequently, the extract obtained from repeatedly extracting fresh rosemary leaves is heated and spray-dried to form spherical microparticles containing lactometramine molecular machinery, plant cellulose, polyphenolic compounds, and other active substances. These spherical microparticles are a multi-component molecular machinery composition with a relatively uniform particle size distribution. They are stable and possess excellent abilities to promote VEGF (growth factor) secretion from dermal papilla cells and inhibit the secretion of IL-1β (growth inhibitory factor) and IL-6 (inflammatory factor). Ultimately, they can soothe inflammation, promote the proliferation and migration of dermal papilla cells, regulate the cyclical movement of hair follicles, and promote hair growth.

[0007] The present invention discloses a method for preparing a rosemary leaf extract rich in lactosmatrine molecular machinery, the specific preparation steps of which are as follows:

[0008] (1) Wash the rosemary leaves with clean water, crush and sieve them to obtain rosemary leaf powder, and store it at low temperature for later use.

[0009] (2) Preparation of lactose-matrine molecular apparatus: lactose and matrine are added to a reactor at a certain molar ratio, and then water is added in an appropriate proportion and stirred until the solution is clear. The solution is then placed on a rotary evaporator for rotary evaporation. The residual liquid after rotary evaporation is lactose-matrine molecular apparatus. A certain amount of ultrapure water is added to the lactose-matrine molecular apparatus to prepare an extract of lactose-matrine molecular apparatus with a suitable extraction concentration.

[0010] (3) Add rosemary leaf powder to the lactose matrine molecular machine extract at a certain solid-liquid ratio to obtain an extraction system. Place the extraction system in a microwave synthesizer, set a certain power and time, and then extract. After completion, let it stand and cool to room temperature to obtain rosemary leaf extract.

[0011] (4) Centrifuge the rosemary leaf extract, filter the supernatant, remove the residue, collect the filtrate, add the rosemary leaf powder again according to the same solid-liquid ratio, and repeat the extraction multiple times according to the same steps.

[0012] (5) The extract obtained by repeatedly extracting rosemary leaves was heated and then spray-dried using a spray dryer to obtain spherical microparticles.

[0013] (6) The obtained spherical particles are dried to obtain rosemary leaf extract rich in lactosmatrine molecular machinery.

[0014] Furthermore, in step (1), the sieve used during sieving is 40-60 mesh, and the low-temperature storage temperature is -10℃ to -20℃.

[0015] Furthermore, in step (2), lactose and matrine are added to the reactor at a molar ratio of 1:0.5 to 1:4, the rotary evaporation temperature is 40 to 80°C, and the rotary evaporation time is 3 to 8 hours; a certain amount of ultrapure water is added to the lactose-matrine molecular machine to prepare a lactose-matrine molecular machine extract with a concentration of 5% to 80%.

[0016] Furthermore, in step (2), a certain amount of ultrapure water is added to the lactosmatrine molecular machine to prepare a lactosmatrine molecular machine extract with a concentration of 50%.

[0017] Furthermore, in step (3), during extraction, the mass-to-volume ratio of rosemary leaf powder to lactosmatrine molecular extract is 1 g:(10-60 mL), and the mixture is treated with microwave power of 200-600 W for 2-12 min.

[0018] Furthermore, in step (3), the processing time is 8 minutes at a microwave power of 200-600W.

[0019] Furthermore, in step (4), the centrifugation speed is 4000-10000 r / min, the time is 3-12 min, the filter membrane is a 0.45 μm organic phase filter membrane, and the rosemary leaves are extracted 1-8 times.

[0020] Furthermore, in step (4), the rosemary leaves are extracted three times.

[0021] Furthermore, in step (5), the spray pressure of the spray dryer is 5-20 kPa, the air volume is 0.2-0.75 m3 / min, the solution flow rate is 200-300 mL / h, the inlet temperature is 110-130℃, and the outlet temperature is 60-90℃.

[0022] Furthermore, in step (6), the drying temperature is 50°C and the drying time is 12 hours.

[0023] An application of rosemary leaf extract rich in lactometramine molecular machinery, including as a daily chemical raw material, where lactometramine molecular machinery does not need to be separated, and works synergistically with polyphenolic compounds including rosmarinic acid to exert excellent antibacterial, anti-inflammatory, antioxidant, hair papilla cell proliferation and migration, regulate hair follicle cycle and promote hair growth effects.

[0024] By means of the above-described solution, the present invention has at least the following advantages:

[0025] (1) This invention uses a microwave-assisted extraction process coupled with the prepared lactometabolite molecular machinery to extract polyphenolic compounds from rosemary leaves. As the concentration of the lactometabolite molecular machinery increases from 10 wt% to 50%, the polyphenol yield significantly increases, followed by a slight decrease with further increases in concentration. This is because as the concentration of the lactometabolite molecular machinery increases, more molecular machinery interacts with the polyphenolic compounds, increasing the solubility of the active ingredients in the entire system, thus increasing the yield. However, when the concentration of the lactometabolite molecular machinery is too high, the viscosity becomes too large, severely affecting mass transfer efficiency, increasing costs while decreasing the yield. Therefore, a 50 wt% lactometabolite molecular machinery was ultimately selected for extraction. The molecular extraction of polyphenols using lactose matrine at different concentrations was superior to that using traditional water and ethanol solvents. The highest extraction efficiency was achieved at a concentration of 50 wt%, reaching 60.24 mg / g, which is 5.9 times that of water extraction (10.21 mg / g), 3.3 times that of ethanol (18.14 mg / g), and 1.5 times that of 50 wt% ethanol (40.31 mg / g), indicating broad market application prospects.

[0026] (2) Microwave-assisted extraction for 2-10 min was used for investigation. As extraction time gradually increased, the polyphenol yield gradually increased, then showed a slight decreasing trend. The increase in polyphenol yield was due to the extended microwave time; the biological and thermal effects of microwaves accelerated the destruction of cell walls, promoting the release, diffusion, and dissolution of intracellular active substances into the solvent. However, the decrease in polyphenol yield could be attributed to excessively long microwave times, during which instantaneous extreme temperatures and pressures could lead to the decomposition of phenolic compounds. The polyphenol content obtained by lactose-matrine molecular machine extraction for 2 min was far superior to that obtained by water and ethanol extraction for 8 min. The combination of lactose-matrine molecular machine extraction and microwave-assisted technology achieved rapid extraction in a short time, maximizing the enrichment of polyphenolic compounds in just 8 min. Their use can reduce waste, simplify the process, and save energy and reduce emissions.

[0027] (3) When extracting polyphenolic compounds, the lactomatrine molecular machine selectively extracts rosmarinic acid, and the content of rosmarinic acid is relatively high. Through practice and quantum chemical calculations, it is verified that the lactomatrine molecular machine has a better specific recognition ability for rosmarinic acid.

[0028] (4) When extracted with 50wt% ethanol three or more times, the polyphenol concentration reaches its limit, the solvent is basically saturated, and the solubility is limited. When lactosmatrine molecular extraction is used, the concentration of polyphenolic compounds increases effectively with the number of extractions, without reaching saturation, and the increase is approximately double. Lactosmatrine molecular extraction has high solubility for active substances and good reusability.

[0029] (5) Through spray drying, spherical microparticles are formed by spray drying of active substances such as lactose-matrine molecular machinery, plant cellulose, and polyphenolic compounds. Lactose has the functions of filling, binding, and shaping. The formed lactose-matrine molecular machinery provides a spherical scaffold, and the active substances are firmly adsorbed around it through intermolecular forces, which facilitates the extraction and stable existence of the active substances.

[0030] (6) The optimized structures of the lactoferrin molecular machine and rosmarinic acid are located at local minima on the potential energy surface, allowing for stable existence. The distance between the carbonyl group of matrine and the hydroxyl group of rosmarinic acid is much shorter than the van der Waals radius, as is the distance between the hydroxyl group of lactose and the carbonyl group of rosmarinic acid. This indicates that there is a hydrogen bond interaction between the lactoferrin molecular machine and rosmarinic acid. When forming a stable structure, the electronegative region of the lactoferrin molecular machine and the electronegative region of rosmarinic acid attract each other, resulting in an overall electrostatic potential close to 0, thus forming a stable structure. There is a van der Waals force between the lactoferrin molecular machine and rosmarinic acid. Simultaneously, there is also a van der Waals force between the hydroxyl group in the lactoferrin molecular machine and the carbonyl group of rosmarinic acid. Therefore, there is a strong hydrogen bond interaction between them at these two sites, which contributes to the formation and stability of the entire system. Therefore, the extraction efficiency of polyphenolic compounds including rosmarinic acid in the lactoferrin molecular machine system of this invention is high.

[0031] (7) The prepared rosemary leaf extract can be used as a new type of daily chemical raw material. The lactose matrine molecular machinery does not need to be separated. It works synergistically with polyphenolic compounds, including rosmarinic acid, to exert excellent antibacterial, anti-inflammatory, antioxidant, and hair papilla cell proliferation and migration effects, regulate the periodic cycle of hair follicles, and promote hair growth.

[0032] The above description is merely an overview of the technical solution of the present invention. In order to better understand the technical means of the present invention and to implement it in accordance with the contents of the specification, the preferred embodiments of the present invention are described in detail below with reference to the accompanying drawings. Attached Figure Description

[0033] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show a certain embodiment of the present invention and should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0034] Figure 1 is a flowchart of a method for preparing a rosemary leaf extract rich in lactosmatrine molecular machines according to the present invention.

[0035] Figure 2 shows (a) structural optimization, (b) ESP potential analysis, and (c) IRI analysis of the lactosmatrine molecular machine of the present invention.

[0036] Figure 3 shows a comparison of the polyphenol extraction effects of different solvents according to the present invention;

[0037] Figure 4 shows a comparison of the effects of different solvents on the extraction of rosmarinic acid according to the present invention;

[0038] Figure 5 shows the molecular machine of lactosmatrine and rosmarinic acid of the present invention: (a) structural optimization; (b) ESP potential analysis; (c) IRI analysis.

[0039] Figure 6 shows the effect of different extraction times on polyphenol yield in this invention;

[0040] Figure 7 shows the effect of the number of repeated extractions on the polyphenol content in this invention;

[0041] Figure 8 is a microscopic morphology diagram of the rosemary leaf extract of the present invention;

[0042] Figure 9 shows the effect of the rosemary leaf extract of the present invention on the activity of dermal papilla cells;

[0043] Figure 10 shows the effect of the rosemary leaf extract of the present invention on the VEGF (growth factor) content;

[0044] Figure 11 shows the effect of the rosemary leaf extract of the present invention on the content of IL-6 (inflammatory factor);

[0045] Figure 12 shows the effect of the rosemary leaf extract of the present invention on the content of IL-1β (growth inhibitory factor). Detailed Implementation

[0046] The specific embodiments of the present invention will be described in further detail below with reference to the accompanying drawings and examples. The following examples are for illustrative purposes only and are not intended to limit the scope of the invention.

[0047] Specific embodiments, comparative examples, and test examples of the present invention are as follows:

[0048] Test Example 1: Quantum Chemical Computational Analysis of Lactosmatrine Molecular Machine

[0049] Figure 2 shows the following data for the molecular machine of lactose-matrine: (a) structural optimization; (b) ESP potential analysis; and (c) IRI analysis. As shown in Figure 2(a), both geometric optimization and frequency calculations converged, and no imaginary frequencies were found, indicating that the current structure is at a local minimum on the potential surface and can exist stably. The hydroxyl groups of lactose and the carbonyl groups of matrine exhibit hydrogen-bonded interactions. The formation mechanism of the lactose-matrine molecular machine was then qualitatively analyzed using Figure 2(b) ESP. Figure 2(b) shows that for matrine, the carbonyl region is electronegative, while a large region surrounding the nitrogen atom is electronegative. For lactose, the hydroxyl region is electronegative. After the formation of the molecular machine, the electronegative region of matrine and the electronegative region of lactose attract each other, forming a stable molecular machine structure. The van der Waals forces, hydrogen bonds, and steric repulsion of the molecule were characterized using the Interaction Region Indicator (IRI) function, as shown in Figure 2(c). In the interaction relationships, blue represents strong attractive interactions such as hydrogen bonds, and red represents strong steric hindrance. The stronger the steric hindrance, the more difficult it is for the atoms in the corresponding two regions to attract each other; the large green transitional regions represent weak van der Waals interactions. The large green interaction region between matrine and lactose indicates the presence of van der Waals forces. Furthermore, the presence of small blue discs between the hydroxyl and carbonyl groups indicates strong hydrogen bond interactions.

[0050] Example 1: Preparation of Rosemary Leaf Extract

[0051] (1) Wash the rosemary leaves with clean water, crush them, sieve them through a 60-mesh sieve, and store them at a low temperature of -10℃.

[0052] (2) Preparation of lactose-matrine molecular solvent: lactose and matrine were added to the reactor in a 1:1 molar ratio, and then water was added in an appropriate proportion and stirred until the solution was clear. The solution was then placed on a rotary evaporator and evaporated at 60°C for 5 hours. The residual liquid after rotary evaporation was lactose-matrine molecular solvent. A certain amount of ultrapure water was added to the lactose-matrine molecular solvent to prepare a 10wt% lactose-matrine molecular solvent solution for extraction.

[0053] (3) Add rosemary leaf powder to the prepared lactose matrine molecular machine solution at a solid-liquid ratio of 1g:40mL and treat it at microwave power of 400W for 8min.

[0054] (4) Centrifuge the extract, filter the supernatant to remove excess impurities, and obtain the extract; the centrifugation parameters are 8000 r / min and 10 min. The filter membrane is a 0.45 μm organic phase filter membrane.

[0055] Example 2: Preparation of Rosemary Leaf Extract

[0056] (1) Wash the rosemary leaves with clean water, crush them, sieve them through a 60-mesh sieve, and store them at a low temperature of -10℃.

[0057] (2) Preparation of lactose-matrine molecular solvent: lactose and matrine were added to the reactor in a 1:1 molar ratio, and then water was added in an appropriate proportion and stirred until the solution was clear. The solution was then placed on a rotary evaporator and evaporated at 60°C for 5 hours. The residual liquid after rotary evaporation was lactose-matrine molecular solvent. A certain amount of ultrapure water was added to the lactose-matrine molecular solvent to prepare a 20wt% lactose-matrine molecular solvent solution for extraction.

[0058] (3) Add rosemary leaf powder to the prepared lactose matrine molecular machine solution at a solid-liquid ratio of 1g:40mL and treat it at microwave power of 400W for 8min.

[0059] (4) Centrifuge the extract, filter the supernatant to remove excess impurities, and obtain the extract; the centrifugation parameters are 8000 r / min and 10 min. The filter membrane is a 0.45 μm organic phase filter membrane.

[0060] Example 3: Preparation of Rosemary Leaf Extract

[0061] (1) Wash the rosemary leaves with clean water, crush them, sieve them through a 60-mesh sieve, and store them at a low temperature of -10℃.

[0062] (2) Preparation of lactose-matrine molecular solvent: lactose and matrine were added to the reactor in a 1:1 molar ratio, and then water was added in an appropriate proportion and stirred until the solution was clear. The solution was then placed on a rotary evaporator and evaporated at 60°C for 5 hours. The residual liquid after rotary evaporation was lactose-matrine molecular solvent. A certain amount of ultrapure water was added to the lactose-matrine molecular solvent to prepare a 30wt% lactose-matrine molecular solvent solution for extraction.

[0063] (3) Add rosemary leaf powder to the prepared lactose matrine molecular machine solution at a solid-liquid ratio of 1g:40mL and treat it at microwave power of 400W for 8min.

[0064] (4) Centrifuge the extract, filter the supernatant to remove excess impurities, and obtain the extract; the centrifugation parameters are 8000 r / min and 10 min. The filter membrane is a 0.45 μm organic phase filter membrane.

[0065] Example 4: Preparation of Rosemary Leaf Extract

[0066] (1) Wash the rosemary leaves with clean water, crush them, sieve them through a 60-mesh sieve, and store them at a low temperature of -10℃.

[0067] (2) Preparation of lactose-matrine molecular solvent: lactose and matrine were added to the reactor in a 1:1 molar ratio, and then water was added in an appropriate proportion and stirred until the solution was clear. The solution was then placed on a rotary evaporator and evaporated at 60°C for 5 hours. The residual liquid after rotary evaporation was lactose-matrine molecular solvent. A certain amount of ultrapure water was added to the lactose-matrine molecular solvent to prepare a 40wt% lactose-matrine molecular solvent solution for extraction.

[0068] (3) Add rosemary leaf powder to the prepared lactose matrine molecular machine solution at a solid-liquid ratio of 1g:40mL and treat it at microwave power of 400W for 8min.

[0069] (4) Centrifuge the extract, filter the supernatant to remove excess impurities, and obtain the extract; the centrifugation parameters are 8000 r / min and 10 min. The filter membrane is a 0.45 μm organic phase filter membrane.

[0070] Example 5: Preparation of Rosemary Leaf Extract

[0071] (1) Wash the rosemary leaves with clean water, crush them, sieve them through a 60-mesh sieve, and store them at a low temperature of -10℃.

[0072] (2) Preparation of lactose-matrine molecular solvent: lactose and matrine were added to the reactor in a 1:1 molar ratio, and then water was added in an appropriate proportion and stirred until the solution was clear. The solution was then placed on a rotary evaporator and evaporated at 60°C for 5 hours. The residual liquid after rotary evaporation was lactose-matrine molecular solvent. A certain amount of ultrapure water was added to the lactose-matrine molecular solvent to prepare a 50wt% lactose-matrine molecular solvent solution for extraction.

[0073] (3) Add rosemary leaf powder to the prepared lactose matrine molecular machine solution at a solid-liquid ratio of 1g:40mL and treat it at microwave power of 400W for 8min.

[0074] (4) Centrifuge the extract, filter the supernatant to remove excess impurities, and obtain the extract; the centrifugation parameters are 8000 r / min and 10 min. The filter membrane is a 0.45 μm organic phase filter membrane.

[0075] Example 6: Preparation of Rosemary Leaf Extract

[0076] (1) Wash the rosemary leaves with clean water, crush them, sieve them through a 60-mesh sieve, and store them at a low temperature of -10℃.

[0077] (2) Preparation of lactose-matrine molecular solvent: lactose and matrine were added to the reactor in a 1:1 molar ratio, and then water was added in an appropriate proportion and stirred until the solution was clear. The solution was then placed on a rotary evaporator and evaporated at 60°C for 5 hours. The residual liquid after rotary evaporation was lactose-matrine molecular solvent. A certain amount of ultrapure water was added to the lactose-matrine molecular solvent to prepare a 60wt% lactose-matrine molecular solvent solution for extraction.

[0078] (3) Add rosemary leaf powder to the prepared lactose matrine molecular machine solution at a solid-liquid ratio of 1g:40mL and treat it at microwave power of 400W for 8min.

[0079] (4) Centrifuge the extract, filter the supernatant to remove excess impurities, and obtain the extract; the centrifugation parameters are 8000 r / min and 10 min. The filter membrane is a 0.45 μm organic phase filter membrane.

[0080] Comparative Example 1: Traditional Rosemary Leaf Extract (Water)

[0081] (1) Wash the rosemary leaves with clean water, crush them, sieve them through a 60-mesh sieve, and store them at a low temperature of -10℃.

[0082] (2) Add rosemary leaf powder to ultrapure water at a solid-liquid ratio of 1g:40mL and treat it with microwave power of 400W for 8min.

[0083] (3) Centrifuge the extract, filter the supernatant to remove excess impurities, and obtain the extract; the centrifugation parameters are 8000 r / min and 10 min. The filter membrane is a 0.45 μm organic phase filter membrane.

[0084] Comparative Example 2: Traditional rosemary leaf extract (25 wt% ethanol)

[0085] (1) Wash rosemary leaves with clean water, freeze dry at -60℃ for 5 hours, pulverize, and sieve through 60 mesh;

[0086] (2) Add rosemary leaf powder to 25wt% ethanol at a solid-liquid ratio of 1g:40mL and treat it with microwave power of 400W for 8min.

[0087] (3) Centrifuge the extract, filter the supernatant to remove excess impurities, and obtain the extract; the centrifugation parameters are 8000 r / min and 10 min. The filter membrane is a 0.45 μm organic phase filter membrane.

[0088] Comparative Example 3: Traditional rosemary leaf extract (50 wt% ethanol)

[0089] (1) Wash rosemary leaves with clean water, freeze dry at -60℃ for 5 hours, pulverize, and sieve through 60 mesh;

[0090] (2) Add rosemary leaf powder to 50wt% ethanol at a solid-liquid ratio of 1g:40mL and treat it with microwave power of 400W for 8min.

[0091] (3) Centrifuge the extract, filter the supernatant to remove excess impurities, and obtain the extract; the centrifugation parameters are 8000 r / min and 10 min. The filter membrane is a 0.45 μm organic phase filter membrane.

[0092] Comparative Example 4: Traditional rosemary leaf extract (75 wt% ethanol)

[0093] (1) Wash rosemary leaves with clean water, freeze dry at -60℃ for 5 hours, pulverize, and sieve through 60 mesh;

[0094] (2) Add rosemary leaf powder to 75wt% ethanol at a solid-liquid ratio of 1g:40mL and treat it with microwave power of 400W for 8min.

[0095] (3) Centrifuge the extract, filter the supernatant to remove excess impurities, and obtain the extract; the centrifugation parameters are 8000 r / min and 10 min. The filter membrane is a 0.45 μm organic phase filter membrane.

[0096] Comparative Example 5: Traditional Rosemary Leaf Extract (Ethanol)

[0097] (1) Wash rosemary leaves with clean water, freeze dry at -60℃ for 5 hours, pulverize, and sieve through 60 mesh;

[0098] (2) Add rosemary leaf powder to ethanol at a solid-liquid ratio of 1g:40mL and treat with microwave power of 400W for 8min.

[0099] (3) Centrifuge the extract, filter the supernatant to remove excess impurities, and obtain the extract; the centrifugation parameters are 8000 r / min and 10 min. The filter membrane is a 0.45 μm organic phase filter membrane.

[0100] Test Example 2: Effect of different solvents on the yield of polyphenols and rosmarinic acid

[0101] The filtrates obtained from one extraction in Examples 1-6 and Comparative Examples 1-5 were analyzed for polyphenolic compounds and rosmarinic acid content. The specific test conditions are as follows:

[0102] (1) Polyphenol content determination: The concentration of polyphenolic compounds in the extract was quantitatively determined using a Shimadzu UV-1900i series ultraviolet spectrophotometer at a measurement wavelength of 765 nm.

[0103] (2) The content of rosmarinic acid was determined by HPLC with a C18 column (4.6 mm × 250 mm, 5 μm), a column temperature of 25 °C, a detection wavelength of 330 nm, a mobile phase of acetonitrile-methanol-0.1% phosphoric acid solution (18:16:66), and a flow rate of 1 mL / min.

[0104] Figure 3 shows the polyphenol yield test results, comparing the extraction effects of different solvents. With increasing ethanol concentration, the polyphenol yield initially increased and then decreased. The highest yield was achieved with 50 wt% ethanol. This is because the addition of ethanol alters the polarity of the original system; at a concentration of 50 wt%, the polarity best matches the active polyphenolic substances, resulting in the highest extraction efficiency. However, in general, the traditional combination of ethanol and water has limited solubility and extraction effect, and the use of ethanol presents issues of flammability, explosiveness, and subsequent separation and detection costs. As the concentration of lactometramine molecular apparatus increased from 10 wt% to 50%, the polyphenol yield significantly improved, then showed a slight decreasing trend with further concentration increases. This is because as the concentration of lactometramine molecular apparatus increases, more molecular apparatuses interact with polyphenolic compounds, increasing the solubility of the active ingredients in the entire system, thus increasing the yield. However, when the concentration of lactometramine molecular apparatus is too high, the viscosity becomes excessive, severely affecting mass transfer efficiency, increasing costs while decreasing the yield. Therefore, 50 wt% lactometramine molecular apparatus was ultimately selected for further investigation. In summary, the molecular extraction of polyphenols using lactostatine at different concentrations is superior to that using traditional water and ethanol solvents. The highest extraction efficiency, reaching 60.24 mg / g, is achieved at a concentration of 50 wt%, which is 5.9 times that of water extraction (10.21 mg / g), 3.3 times that of ethanol (18.14 mg / g), and 1.5 times that of 50 wt% ethanol (40.31 mg / g), indicating broad market application prospects.

[0105] The rosmarinic acid yield test results are shown in Figure 4. In different concentrations of ethanol and lactoferrin molecular systems, the yield of rosmarinic acid followed the same trend as that of polyphenols. The extraction efficiency was highest at a lactoferrin molecular concentration of 50 wt%, reaching 23.12 mg / g, which is 20.6 times that of water extraction (1.12 mg / g), 4.5 times that of ethanol (5.14 mg / g), and 1.9 times that of 50 wt% ethanol (12.14 mg / g). By comparing the polyphenol yield ratios, it can be concluded that lactoferrin molecular systems selectively extract rosmarinic acid when extracting polyphenolic compounds, resulting in a higher proportion of rosmarinic acid content. This verifies that lactoferrin molecular systems have a better specific recognition ability for rosmarinic acid.

[0106] The lactometramine molecular machine not only has higher polyphenol extraction efficiency, but also has better specific recognition ability for the functional compound rosmarinic acid.

[0107] Test Example 3: Quantum Chemical Calculation of the Mechanism of Lactosidase Molecular Extraction of Rosmarinic Acid

[0108] The potential mechanism for the high extraction efficiency of polyphenolic compounds, including rosmarinic acid, in the lactosmatrine molecular machine system was investigated using quantum chemical calculations. Using rosmarinic acid as the computational model, the form and strength of the interaction force between the lactosmatrine molecular machine and rosmarinic acid were analyzed. The optimized structures of the lactosmatrine molecular machine and rosmarinic acid are shown in Figure 5(a). Both geometric optimization and frequency calculations converged, and no imaginary frequencies were found, indicating that the current structure is at a local minimum on the potential energy surface and can exist stably. The distance between the carbonyl group of matrine and the hydroxyl group of rosmarinic acid is much shorter than the van der Waals radius, as is the distance between the hydroxyl group of lactose and the carbonyl group of rosmarinic acid. This indicates that there is a hydrogen bond interaction between the lactosmatrine molecular machine and rosmarinic acid. As shown in Figure 5(b), the carbonyl regions of matrine and rosmarinic acid are electronegative, while the hydroxyl groups are electronegative. During the formation of a stable structure, the electronegative regions of the lactometabolite molecular machine and the electronegative regions of rosmarinic acid attract each other, resulting in a white binding region and an overall electrostatic potential close to zero, thus forming a stable structure. Figure 5(c) shows the indicator function analysis of the interaction region between the lactometabolite molecular machine and rosmarinic acid. Large, green, sheet-like interaction regions exist between the lactometabolite molecular machine and rosmarinic acid, indicating the presence of van der Waals forces. Simultaneously, blue discs exist between the carbonyl group in the lactometabolite molecular machine and the hydroxyl group on the carboxyl group of rosmarinic acid, as do the hydroxyl group in the lactometabolite molecular machine and the carbonyl group of rosmarinic acid. This implies the existence of strong hydrogen bond interactions at these two sites, which contributes to the formation and stability of the entire system. The higher yields of rosmarinic acid and polyphenols in the lactometabolite molecular machine system can be explained by ESP, structural optimization, and IRI.

[0109] Example 7: Preparation of Rosemary Leaf Extract

[0110] (1) Wash the rosemary leaves with clean water, crush them, sieve them through a 60-mesh sieve, and store them at a low temperature of -10℃.

[0111] (2) Preparation of lactose-matrine molecular solvent: lactose and matrine were added to the reactor in a 1:1 molar ratio, and then water was added in an appropriate proportion and stirred until the solution was clear. The solution was then placed on a rotary evaporator and evaporated at 60°C for 5 hours. The residual liquid after rotary evaporation was lactose-matrine molecular solvent. A certain amount of ultrapure water was added to the lactose-matrine molecular solvent to prepare a 50wt% lactose-matrine molecular solvent solution for extraction.

[0112] (3) Add rosemary leaf powder to the prepared lactose matrine molecular machine solution at a solid-liquid ratio of 1g:40mL and treat it under microwave power of 400W for 2min.

[0113] (4) Centrifuge the extract, filter the supernatant to remove excess impurities, and obtain the extract; the centrifugation parameters are 8000 r / min and 10 min. The filter membrane is a 0.45 μm organic phase filter membrane.

[0114] Example 8: Preparation of Rosemary Leaf Extract

[0115] (1) Wash the rosemary leaves with clean water, crush them, sieve them through a 60-mesh sieve, and store them at a low temperature of -10℃.

[0116] (2) Preparation of lactose-matrine molecular solvent: lactose and matrine were added to the reactor in a 1:1 molar ratio, and then water was added in an appropriate proportion and stirred until the solution was clear. The solution was then placed on a rotary evaporator and evaporated at 60°C for 5 hours. The residual liquid after rotary evaporation was lactose-matrine molecular solvent. A certain amount of ultrapure water was added to the lactose-matrine molecular solvent to prepare a 50wt% lactose-matrine molecular solvent solution for extraction.

[0117] (3) Add rosemary leaf powder to the prepared lactose matrine molecular machine solution at a solid-liquid ratio of 1g:40mL and treat it at microwave power of 400W for 4min.

[0118] (4) Centrifuge the extract, filter the supernatant to remove excess impurities, and obtain the extract; the centrifugation parameters are 8000 r / min and 10 min. The filter membrane is a 0.45 μm organic phase filter membrane.

[0119] Example 9: Preparation of Rosemary Leaf Extract

[0120] (1) Wash the rosemary leaves with clean water, crush them, sieve them through a 60-mesh sieve, and store them at a low temperature of -10℃.

[0121] (2) Preparation of lactose-matrine molecular solvent: lactose and matrine were added to the reactor in a 1:1 molar ratio, and then water was added in an appropriate proportion and stirred until the solution was clear. The solution was then placed on a rotary evaporator and evaporated at 60°C for 5 hours. The residual liquid after rotary evaporation was lactose-matrine molecular solvent. A certain amount of ultrapure water was added to the lactose-matrine molecular solvent to prepare a 50wt% lactose-matrine molecular solvent solution for extraction.

[0122] (3) Add rosemary leaf powder to the prepared lactose matrine molecular machine solution at a solid-liquid ratio of 1g:40mL and treat it under microwave power of 400W for 6min.

[0123] (4) Centrifuge the extract, filter the supernatant to remove excess impurities, and obtain the extract; the centrifugation parameters are 8000 r / min and 10 min. The filter membrane is a 0.45 μm organic phase filter membrane.

[0124] Example 10: Preparation of Rosemary Leaf Extract

[0125] (1) Wash the rosemary leaves with clean water, crush them, sieve them through a 60-mesh sieve, and store them at a low temperature of -10℃.

[0126] (2) Preparation of lactose-matrine molecular solvent: lactose and matrine were added to the reactor in a 1:1 molar ratio, and then water was added in an appropriate proportion and stirred until the solution was clear. The solution was then placed on a rotary evaporator and evaporated at 60°C for 5 hours. The residual liquid after rotary evaporation was lactose-matrine molecular solvent. A certain amount of ultrapure water was added to the lactose-matrine molecular solvent to prepare a 50wt% lactose-matrine molecular solvent solution for extraction.

[0127] (3) Add rosemary leaf powder to the prepared lactose matrine molecular machine solution at a solid-liquid ratio of 1g:40mL and treat it with microwave power of 400W for 10min.

[0128] (4) Centrifuge the extract, filter the supernatant to remove excess impurities, and obtain the extract; the centrifugation parameters are 8000 r / min and 10 min. The filter membrane is a 0.45 μm organic phase filter membrane.

[0129] Test Example 4: Effect of different microwave-assisted extraction times on the yield of polyphenolic compounds

[0130] The filtrates obtained from extractions in Examples 5 and 7-10 were analyzed for polyphenol content, with the same testing conditions as in Example 1. The results are shown in Figure 6, illustrating the effect of different microwave-assisted extraction times on polyphenol yield. The experiment investigated the effect using microwave-assisted extraction times of 2-10 min. As the extraction time gradually increased, the polyphenol yield gradually increased, then showed a slight decreasing trend. The increase in polyphenol content with increasing extraction time is due to the fact that the extended microwave time accelerates the destruction of cell walls through the biological and thermal effects of microwaves, promoting the release, diffusion, and dissolution of intracellular active substances into the solvent. However, the decrease in polyphenol yield can be attributed to excessively long microwave times, during which instantaneous extreme temperatures and pressures can lead to the decomposition of phenolic compounds. Furthermore, comparing the polyphenol yields in Example 7 with those in Comparative Examples 1 and 5, the polyphenol content obtained from 2 min of molecular extraction with lactometabolite was significantly higher than that obtained from 8 min of water or ethanol extraction. The combination of lactose matrine molecular machinery and microwave-assisted technology has the effect of rapid extraction in a short time, achieving maximum enrichment of polyphenolic compounds in just 8 minutes. Their use can reduce waste, simplify the process, and save energy and reduce emissions.

[0131] Example 11: Preparation of rosemary leaf extract (extraction repeated 5 times)

[0132] (1) Wash the rosemary leaves with clean water, crush them, sieve them through a 60-mesh sieve, and store them at a low temperature of -10℃.

[0133] (2) Preparation of lactose-matrine molecular solvent: lactose and matrine were added to the reactor in a 1:1 molar ratio, and then water was added in an appropriate proportion and stirred until the solution was clear. The solution was then placed on a rotary evaporator and evaporated at 60°C for 5 hours. The residual liquid after rotary evaporation was lactose-matrine molecular solvent. A certain amount of ultrapure water was added to the lactose-matrine molecular solvent to prepare a 50wt% lactose-matrine molecular solvent solution for extraction.

[0134] (3) Add rosemary leaf powder to the prepared lactose matrine molecular machine solution at a solid-liquid ratio of 1g:40mL and treat it at microwave power of 400W for 8min.

[0135] (4) Centrifuge the above extract, filter the supernatant to remove excess impurities, collect the filtrate, add new rosemary leaf powder according to the same solid-liquid ratio, and re-extract polyphenolic compounds according to the same process. This extraction process is repeated 5 times.

[0136] Comparative Example 6: Traditional Rosemary Leaf Extract (Water) (Extraction repeated 5 times)

[0137] (1) Wash the rosemary leaves with clean water, crush them, sieve them through a 60-mesh sieve, and store them at a low temperature of -10℃.

[0138] (2) Add rosemary leaf powder to ultrapure water at a solid-liquid ratio of 1g:40mL and treat it with microwave power of 400W for 8min.

[0139] (3) Centrifuge the above extract, filter the supernatant to remove excess impurities, collect the filtrate, add new rosemary leaf powder according to the same solid-liquid ratio, and re-extract polyphenolic compounds according to the same process. This extraction process is repeated 5 times.

[0140] Test Example 5: Effect of the Number of Repeated Extractions on Polyphenol Content

[0141] The polyphenol content of each filtrate from repeated extractions in Example 11 and Comparative Example 6 was determined, and the results are shown in Figure 7, representing the polyphenol concentration at different extraction times. In Comparative Example 6, the polyphenol concentration gradually increased with the number of extractions, but reached its limit after three or more extractions, indicating that the 50 wt% ethanol in the system was essentially saturated, limiting its solubility. In contrast, in Example 11, the concentration of polyphenolic compounds increased effectively with each extraction, without reaching saturation, and the increase was approximately double. This demonstrates the high solubility and good reusability of the lactometabolite molecular machinery for the active substance.

[0142] Example 12: Preparation of Rosemary Leaf Extract

[0143] (1) Wash the rosemary leaves with clean water, crush them, sieve them through a 60-mesh sieve, and store them at a low temperature of -10℃.

[0144] (2) Preparation of lactose-matrine molecular solvent: lactose and matrine were added to the reactor in a 1:1 molar ratio, and then water was added in an appropriate proportion and stirred until the solution was clear. The solution was then placed on a rotary evaporator and evaporated at 60°C for 5 hours. The residual liquid after rotary evaporation was lactose-matrine molecular solvent. A certain amount of ultrapure water was added to the lactose-matrine molecular solvent to prepare a 50wt% lactose-matrine molecular solvent solution for extraction.

[0145] (3) Add rosemary leaf powder to the prepared lactose matrine molecular machine solution at a solid-liquid ratio of 1g:40mL and treat it at microwave power of 400W for 8min.

[0146] (4) Centrifuge the above extract, filter the supernatant to remove excess impurities, collect the filtrate, add new rosemary leaf powder according to the same solid-liquid ratio, and re-extract polyphenolic compounds according to the same process. This extraction process is repeated 5 times.

[0147] (5) The extract obtained from repeatedly extracting fresh rosemary leaves is heated and then spray-dried to form spherical microparticles containing active substances such as lactose-matrine molecular machinery, plant cellulose, and polyphenolic compounds. This powdered granule is the rosemary leaf extract. The spray pressure of the spray dryer is 10 kPa, the air volume is 0.4 m³ / min, the solution flow rate is 300 mL / h, the inlet temperature is 110℃, and the outlet temperature is 70℃.

[0148] Test Example 6: Microscopic Morphology Analysis of Spray-Dried Powder

[0149] The powder obtained after processing in Example 12 was analyzed by scanning electron microscopy, as shown in Figure 8. The powder morphology consists of relatively uniformly sized spherical particles. This surface, after being spray-dried, forms spherical microparticles containing lactose-matrine molecular machinery, plant cellulose, polyphenolic compounds, and other active substances. These spherical microparticles are a multi-component molecular machinery composition with a relatively uniform particle size distribution and stable existence. Lactose has a filling, binding, and shaping effect; the formed lactose-matrine molecular machinery provides a spherical scaffold, firmly adsorbing the active substances around it through intermolecular forces, facilitating the extraction and stable existence of the active substances.

[0150] Test Example 7: Cytotoxicity Test of Rosemary Leaf Extract

[0151] Objective and principle of the test: To detect the effect of the rosemary leaf extract obtained in Example 12 on the toxicity of dermal papilla cells. Cell viability was determined by metabolic activity assay. MTT (thiazolyl blue) was metabolically reduced in living cells to generate insoluble blue-purple formazan. The number of living cells was correlated with the colorimetric value measured by a spectrophotometer after formazan was dissolved in alcohol.

[0152] Figure 9 shows the relative survival rate trend of dermal papilla cells in Test Example 5 of this invention. When the relative cell survival rate is higher than 90%, the sample can be considered to have no biotoxic effect at that concentration and has good biocompatibility. Based on dermal papilla cells, the relative cell survival rate of rosemary leaf extract is higher than 90% within 5 mg / mL, indicating good biocompatibility. Therefore, a test concentration of 5 mg / mL was selected for subsequent tests based on the anti-inflammatory and hair growth factor secretion effects of dermal papilla cells.

[0153] Test Example 8: Determination of VEGF (Growth Factor) Content in Hair Papillary Cells

[0154] Detection Objective and Principle: The mechanism by which VEGF regulates hair follicle growth mainly involves the following two aspects: VEGF can significantly promote the proliferation and migration of dermal papilla cells, and its maximum activity is twice that of basic fibroblast growth factor (bFGF). VEGF may regulate the cyclical movement of hair follicles and promote hair growth by promoting the proliferation and migration of dermal papilla cells. Induction of angiogenesis around hair follicles: The cyclical movement of hair follicles is accompanied by corresponding changes in the blood vessels around the hair follicles. In the early stages of hair growth, a large number of blood vessels form around the hair follicles, while in the regression and resting phases, the blood vessels undergo significant degeneration.

[0155] Detection method: Logarithmic growth phase dermal papilla cells were seeded at a density of 100 cells / well in 24-well plates. After 24 hours, the cells were stably adhered and the culture medium was discarded. The target concentration was selected based on the MTT assay results, and rosemary leaf extract was dissolved in DMEM culture medium to a concentration of 5 mg / mL. The blank control group was treated with only an equal amount of DMEM culture medium and solvent; the positive control group was treated with 500 μM minoxidil, with 6 replicates per concentration. After 72 hours of culture, the medium was replaced with serum-free medium and cultured for another 24 hours. The supernatant was collected, and VEGF levels were detected using a VEGF kit. The experiment was repeated 3 times.

[0156] The test results are shown in Figure 10, which is a graph of VEGF (growth factor) content in dermal papilla cells. The conclusions are as follows:

[0157] Compared with the BC group, the VEGF secretion level in the NC group was significantly lower, indicating that the stimulation conditions in this test were effective.

[0158] Compared with the NC group, the VEGF secretion level in the PC group was significantly increased, indicating that the positive condition of this test was effective.

[0159] Compared with the NC group, rosemary leaf extract significantly increased VEGF levels, with an increase rate of 32%.

[0160] Test Example 9: Determination of IL-6 (inflammatory factor) content in dermal papilla cells

[0161] Purpose and principle of detection: To evaluate the anti-inflammatory efficacy of the samples, this experiment used DHT-induced human dermal papilla cells as an in vitro inflammatory cell model and detected the inflammatory factor (IL-6) secreted in the cell supernatant using an ELISA kit, thereby evaluating the anti-inflammatory efficacy of the samples.

[0162] The detection method is the same as above, and the detection results are shown in Figure 11, which is a graph of IL-6 (inflammatory factor) content in dermal papilla cells. The detection conclusions are as follows:

[0163] Compared with the BC group, the IL-6 secretion level in the NC group was significantly increased, indicating that the stimulation conditions in this test were effective.

[0164] Compared with the NC group, the IL-6 secretion level in the PC group was significantly lower, indicating that the positive condition of this test was effective.

[0165] Compared with the NC group, rosemary leaf extract significantly reduced IL-6 levels, with an inhibition rate of 48%.

[0166] Test Example 10: Determination of IL-1β (growth inhibitory factor) content in dermal papilla cells

[0167] Objective and Principle: The expression of IL-1β, a hair follicle growth inhibitor, shortens the anagen phase of hair follicles, causing them to enter the catagen phase at an earlier stage. This experiment used DHT-induced human dermal papilla cells as an in vitro inflammatory cell model and detected the secreted IL-1β in the cell supernatant using an ELISA kit to evaluate the effect of the sample on hair follicle growth.

[0168] The detection method is the same as above, and the detection results are shown in Figure 12, which is a graph of IL-1β (growth inhibitory factor) content in dermal papilla cells. The detection conclusions are as follows:

[0169] Compared with the BC group, the IL-1β secretion level in the NC group was significantly increased, indicating that the stimulation conditions in this test were effective.

[0170] Compared with the NC group, the IL-1β secretion level in the PC group was significantly reduced, indicating that the positive condition of this test was effective.

[0171] Compared with the NC group, rosemary leaf extract significantly reduced IL-1β levels, with an inhibition rate of 60%.

[0172] In summary, the present invention has the following advantages compared with the prior art:

[0173] (1) The present invention uses the prepared lactomatrine molecular machine coupled microwave-assisted extraction process to extract polyphenolic compounds from rosemary leaves. The extraction effect of lactomatrine molecular machine on polyphenols at different concentrations is better than that of traditional water and ethanol solvents. The extraction efficiency is the highest when the concentration is 50wt%, up to 60.24mg / g, which is 5.9 times that of water extraction (10.21mg / g), 3.3 times that of ethanol (18.14mg / g), and 1.5 times that of 50wt% ethanol (40.31mg / g), and has broad market application prospects.

[0174] (2) The polyphenol content obtained by the lactose matrine molecular machine extraction in 2 minutes is far superior to that obtained by water and ethanol extraction in 8 minutes. The combination of the lactose matrine molecular machine and microwave-assisted process has the effect of short-time and rapid extraction. The maximum enrichment of polyphenolic compounds is achieved in just 8 minutes. Their use can reduce waste, simplify the process and save energy and reduce emissions.

[0175] (3) When extracting polyphenolic compounds, the lactomatrine molecular machine selectively extracts rosmarinic acid, and the content of rosmarinic acid is relatively high. Through practice and quantum chemical calculations, it is verified that the lactomatrine molecular machine has a better specific recognition ability for rosmarinic acid.

[0176] (4) When extracted with 50wt% ethanol three or more times, the polyphenol concentration reaches its limit, the solvent is basically saturated, and the solubility is limited. When lactosmatrine molecular extraction is used, the concentration of polyphenolic compounds increases effectively with the number of extractions, without reaching saturation, and the increase is approximately double. Lactosmatrine molecular extraction has high solubility for active substances and good reusability.

[0177] Through spray drying, spherical microparticles are formed by co-spray drying of active substances such as lactose-matrine molecular machinery, plant cellulose, and polyphenolic compounds. Lactose has the functions of filling, binding, and shaping, while the formed lactose-matrine molecular machinery provides a spherical scaffold, firmly adsorbing the active substances around it through intermolecular forces, which facilitates the extraction and stable existence of the active substances.

[0178] The rosemary leaf extract prepared above can be used as a novel daily chemical raw material. The lactose matrine molecular structure does not need to be separated. It works synergistically with polyphenolic compounds, including rosmarinic acid, to exert excellent antibacterial, anti-inflammatory, antioxidant, and hair papilla cell proliferation and migration effects, regulate hair follicle cycle, and promote hair growth.

[0179] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A method for preparing a rosemary leaf extract rich in lactosmatrine molecular machinery, characterized in that: The specific preparation steps are as follows: (1) Wash the rosemary leaves with clean water, crush and sieve them to obtain rosemary leaf powder, and store it at low temperature for later use. (2) Preparation of lactose-matrine molecular weight: lactose and matrine were added to a reactor at a molar ratio of 1:0.5 to 1:4, and water was added and stirred until the solution was clear. The solution was then placed on a rotary evaporator for rotary evaporation at a temperature of 40 to 80°C for 3 to 8 hours. The residual liquid after rotary evaporation was the lactose-matrine molecular weight. A certain amount of ultrapure water was added to the lactose-matrine molecular weight to prepare an extract of lactose-matrine molecular weight with a concentration of 5% to 80%. (3) The rosemary leaf powder is added to the lactose matrine molecular machine extract at a certain solid-liquid ratio to obtain an extraction system. The extraction system is placed in a microwave synthesizer, and extraction is performed after setting a certain power and time. After completion, the mixture is allowed to stand and cool to room temperature to obtain rosemary leaf extract. During extraction, the mass-to-volume ratio of the rosemary leaf powder to the lactosmatrine molecular extract is 1 g:(10-60 mL), and the mixture is treated with microwave power of 200-600 W for 2-12 min. (4) Centrifuge the rosemary leaf extract, filter the supernatant, remove the filter residue, collect the filtrate, add the rosemary leaf powder again according to the same solid-liquid ratio, and repeat the extraction multiple times according to the same steps. (5) The extract obtained by repeatedly extracting rosemary leaves was heated and then spray-dried using a spray dryer to obtain spherical microparticles. (6) The obtained spherical particles are dried to obtain rosemary leaf extract rich in lactosmatrine molecular machinery.

2. The method for preparing a rosemary leaf extract rich in lactosmatrine molecular machinery according to claim 1, characterized in that: In step (1), the sieve used during sieving is 40-60 mesh, and the low-temperature storage temperature is -10℃ to -20℃.

3. The method for preparing a rosemary leaf extract rich in lactosmatrine molecular machinery according to claim 1, characterized in that: In step (2), a certain amount of ultrapure water is added to the lactosmatrine molecular machine to prepare a lactosmatrine molecular machine extract with a concentration of 50%.

4. The method for preparing a rosemary leaf extract rich in lactosmatrine molecular machinery according to claim 1, characterized in that: In step (3), the processing time is 8 minutes at a microwave power of 200-600W.

5. The method for preparing a rosemary leaf extract rich in lactosmatrine molecular machinery according to claim 1, characterized in that: In step (4), the centrifugation speed is 4000-10000 r / min, the time is 3-12 min, the filter membrane is a 0.45 μm organic phase filter membrane, and the rosemary leaves are extracted 1-8 times.

6. The method for preparing a rosemary leaf extract rich in lactosmatrine molecular machinery according to claim 5, characterized in that: In step (4), the rosemary leaves are extracted three times.

7. The method for preparing a rosemary leaf extract rich in lactosmatrine molecular machinery according to claim 1, characterized in that: In step (5), the spray pressure of the spray dryer is 5-20 kPa, the air volume is 0.2-0.75 m3 / min, the solution flow rate is 200-300 mL / h, the inlet temperature is 110-130℃, and the outlet temperature is 60-90℃.

8. The method for preparing a rosemary leaf extract rich in lactosmatrine molecular machinery according to claim 1, characterized in that: In step (6), the drying temperature is 50°C and the drying time is 12 hours.

Images