Composition for promoting hair growth through activation of hair follicle stem cells

Abstract

The present invention relates to a composition for promoting hair growth through activation of hair follicle stem cells, and a method for manufacturing same. The composition of the present invention has the effect of promoting hair growth or preventing, alleviating, or treating hair loss in an individual through activation of hair follicle stem cells. In addition, since the composition of the present invention consists of herbal medicine extracts, it is possible to minimize side effects of existing chemical therapeutic agents, promote hair growth more safely and effectively, or prevent, alleviate, or treat hair loss.

Description

Composition for promoting hair growth through activation of hair follicle stem cells

[0001] The present invention relates to a composition for promoting hair growth through activation of hair follicle stem cells and a method for producing the same.

[0002] In modern society, hair loss is one of the major cosmetic and psychological problems that many people of all ages and genders experience.

[0003] The above hair loss can be caused by various factors such as genetic factors, hormonal changes, stress, and unbalanced nutrition. In particular, male pattern baldness is caused by androgen, a male hormone, and female pattern baldness can be caused by hormonal imbalance, pregnancy, childbirth, and menopause.

[0004] The hair growth cycle consists of the growth phase (Anagen), the regression phase (Catagen), and the resting phase (Telogen), and hair follicle stem cells play an essential role in maintaining the growth phase of the hair growth cycle and generating new hair.

[0005] However, as we age, the function of hair follicle stem cells declines, the hair growth cycle shortens, and hair loss occurs.

[0006] Existing hair loss treatments primarily consist of drugs that artificially extend the hair growth cycle or stimulate hair growth. Representative drugs include minoxidil and finasteride. While these drugs offer some effectiveness, they can cause side effects and resistance issues.

[0007] To solve these problems, hair loss treatments using natural ingredients are gaining attention.

[0008] Herbal medicines are natural ingredients that have long been used in traditional medicine and are known to promote health through various pharmacological effects. In particular, herbal medicines are considered to have potential efficacy in treating hair loss due to their minimal side effects, safety, and diverse physiological activities.

[0009] The inventors of the present invention recognized the problems of the above-mentioned prior art and completed the present invention by confirming through specific experiments that a composition containing a specific ingredient can promote hair growth and prevent, improve or treat hair loss through activation of hair follicle stem cells.

[0010] The present invention relates to a composition for promoting hair growth, comprising at least one selected from the group consisting of forsythia, rehmannia glutinosa, lotus root, gardenia, pogonyeong, goldenrod, coptis, phellodendron amurense, clove root, chrysanthemum, golsaebo, walnut, seonmo, angelica, astragalus, turmeric, valerian root, fennel and rehmannia glutinosa.

[0011] The present invention,

[0012] The present invention relates to a method for producing a composition for promoting hair growth, which comprises at least one selected from the group consisting of forsythia, rehmannia glutinosa, lotus root, gardenia, pogonyeong, goldenrod, coptis, phellodendron amurense, clove root, chrysanthemum, golsaebo, walnut, seonmo, angelica, astragalus, turmeric, valerian root, fennel and rehmannia glutinosa.

[0013] The present invention,

[0014] A step for preparing a hair growth promoting composition comprising at least one selected from the group consisting of forsythia, raw rehmannia root, lotus root, gardenia, pogonyeong, goldenrod, coptis, phellodendron amurense, clove root, chrysanthemum, golsaebo, walnut meat, seonmo, angelica, astragali, turmeric, valerian root, fennel and rehmannia glutinosa; and

[0015] It relates to a method for promoting hair growth, comprising a step of administering the above composition to an individual.

[0016] This is described in detail below. All combinations of the various elements disclosed in the present invention fall within the scope of the present invention. Furthermore, the scope of the present invention is not limited by the specific description below.

[0017] Additionally, unless otherwise defined herein, all terms used herein should be understood to have the same meaning as commonly understood by one of ordinary skill in the art to which the present invention pertains.

[0018] Also, singular expressions include plural expressions unless the context clearly dictates otherwise.

[0019] In this specification, terms such as "first" and "second" may be used to distinguish or describe one component from another, but these terms should not be interpreted as limiting the components. For example, within the scope of the present invention, a "first" component may be referred to as a "second" component, or vice versa.

[0020] Additionally, in this specification, “degree” combined with a number represents degree Celsius (℃), and may be used interchangeably with “℃” (for example, “100 degrees” is used with the same meaning as “100℃”).

[0021] The present invention provides a composition for promoting hair growth, comprising an extract extracted from at least one selected from the group consisting of forsythia, rehmannia glutinosa, lotus root, gardenia, pogonyeong, scutellaria baicalensis, coptis, phellodendron amurense, clove, rhizome, golsaebo, walnut, seonmo, angelica, astragalus, turmeric, valerian root, fennel and rehmannia glutinosa.

[0022] In the present invention, "Lonicera japonica" refers to a vine plant belonging to the Caprifoliaceae family, native to East Asia, including China, Korea, and Japan. Lonicera japonica is a medicinal herb widely used in Oriental and traditional medicine, and is known to have anti-inflammatory, antibacterial, antipyretic, and detoxifying effects.

[0023] In the present invention, "raw rehmannia glutinosa" refers to the root of a perennial herb belonging to the Scrophulariaceae family, which is used as is. The raw rehmannia glutinosa is known to have antipyretic, detoxifying, blood circulation-improving, and bleeding-inhibiting effects.

[0024] In the present invention, "Forsythia suspensa" refers to a deciduous shrub belonging to the Oleaceae family, native primarily to East Asia, including China, Korea, and Japan. Forsythia suspensa is known to have anti-inflammatory, antibacterial, antiviral, antipyretic, detoxifying, and antioxidant effects.

[0025] In the present invention, "Gardenia jasminoides" refers to an evergreen shrub belonging to the Rubiaceae family, native to East Asia and widely cultivated for ornamental and medicinal purposes. Gardenia jasminoides is known to have anti-inflammatory, antipyretic, diuretic, detoxifying, and antibacterial effects.

[0026] In the present invention, "Taraxacum platycarpum," also known as dandelion, refers to a perennial herb belonging to the Asteraceae family, native primarily to Asia, Europe, and North America. Taraxacum platycarpum is known to have anti-inflammatory, antibacterial, antiviral, detoxifying, diuretic, and digestive-promoting effects.

[0027] In the present invention, "Scutellaria baicalensis" refers to a perennial herbaceous plant belonging to the Lamiaceae family, native primarily to East Asia, including China, Korea, and Japan. Scutellaria baicalensis is known to have anti-inflammatory, antibacterial, antiviral, antioxidant, antipyretic, and hepatoprotective effects.

[0028] In the present invention, "Coptis chinensis" refers to a perennial herbaceous plant belonging to the Ranunculaceae family, native primarily to East Asia, including China, Korea, and Japan. Coptis chinensis is known to have anti-inflammatory, antibacterial, antiviral, antipyretic, analgesic, and digestive-improving effects.

[0029] In the present invention, "Phellodendron amurense" refers to a deciduous broadleaf tree belonging to the Rutaceae family, native primarily to East Asia, including China, Korea, and Japan. Phellodendron amurense is known to have anti-inflammatory, antibacterial, antiviral, antipyretic, detoxifying, analgesic, and liver-protecting effects.

[0030] In the present invention, "Allium fistulosum" refers to a perennial herbaceous plant belonging to the lily family (Liliaceae), native to East Asia, including China, Korea, and Japan. Allium fistulosum is known to have anti-inflammatory, antibacterial, antiviral, antipyretic, immune-boosting, and digestive-promoting effects.

[0031] In the present invention, "Drynaria fortunei" refers to a perennial fern belonging to the Polypodiaceae family, native primarily to East Asian regions such as China, Korea, and Japan. Drynaria fortunei is known to have effects such as bone strengthening, anti-inflammatory, analgesic, hemostatic, and improving kidney function.

[0032] In the present invention, "Juglans regia" refers to the fruit of a walnut tree belonging to the Juglandaceae family, which is native to East Asia, including China, Korea, and Japan. The walnut tree is known to have effects such as improving brain function, antioxidant properties, anti-inflammatory properties, strengthening immunity, improving cardiovascular health, and promoting digestion.

[0033] In the present invention, "Epimedium koreanum" refers to a perennial herbaceous plant belonging to the Berberidaceae family, native to East Asia, including China, Korea, and Japan. Epimedium koreanum is known to have health-enhancing, immune-boosting, anti-inflammatory, antioxidant, blood circulation-improving, and sexual function-enhancing effects.

[0034] In the present invention, "Angelica gigas" refers to a perennial herbaceous plant belonging to the Apiaceae family, native to East Asia, including China, Korea, and Japan. Angelica gigas is known to have effects such as improving blood circulation, anti-inflammatory effects, pain relief, immune enhancement, hormone regulation, and fatigue recovery.

[0035] In the present invention, "Astragalus membranaceus" refers to a perennial herbaceous plant belonging to the legume family (Fabaceae), native to East Asia, including China, Korea, and Japan. Astragalus membranaceus is known to have effects such as strengthening the immune system, anti-inflammatory properties, antioxidant properties, fatigue recovery, improved blood circulation, and liver protection.

[0036] In the present invention, "turmeric (Curcuma longa)" refers to a perennial herbaceous plant belonging to the ginger family (Zingiberaceae), native primarily to India, China, and Southeast Asia. Turmeric is known to have anti-inflammatory, antioxidant, antibacterial, anti-cancer, digestive-promoting, liver-protecting, and immune-boosting effects.

[0037] In the present invention, "Valeriana fauriei" refers to a perennial herbaceous plant belonging to the Valerianaceae family, native to East Asia, including China, Korea, and Japan. The valerian root is known to have sedative, anti-anxiety, sleep-improving, anti-inflammatory, anti-spasmodic, and digestive-promoting effects.

[0038] In the present invention, "Foeniculum vulgare" refers to a perennial or biennial herbaceous plant belonging to the Apiaceae family, native primarily to the Mediterranean region and Asia. The aforementioned Foeniculum vulgare is known to have digestive, anti-inflammatory, antioxidant, antibacterial, diuretic, antispasmodic, and breastfeeding effects.

[0039] In the present invention, "Rehmannia glutinosa" refers to a perennial herbaceous plant belonging to the Scrophulariaceae family, which is native to East Asia, including China, Korea, and Japan. The above-mentioned Rehmannia glutinosa may refer to cooked Rehmannia glutinosa, and unlike raw Rehmannia glutinosa, which uses the roots of Rehmannia glutinosa fresh, cooked Rehmannia glutinosa refers to the roots of Rehmannia glutinosa processed through a process of steaming and drying them several times. The above-mentioned cooked Rehmannia glutinosa has different properties and uses from raw Rehmannia glutinosa, and is known to have effects such as blood replenishment, strengthening, strengthening immunity, improving kidney function, and strengthening physical strength.

[0040] According to one embodiment of the present invention, the composition may include extracts extracted from, but is not limited to, gold and silver flowers, raw rehmannia glutinosa, lotus root, gardenia, pogonyeong, goldenseal, coptis, phellodendron amurense, and rehmannia glutinosa.

[0041] In the present invention, when the composition includes extracts extracted from honeysuckle, raw rehmannia glutinosa, forsythia, gardenia, rhizome of scutellaria baicalensis, Scutellaria baicalensis, Coptis chinensis, Phellodendron amurense and Rehmannia glutinosa, it may include 90 to 110 parts by weight of raw rehmannia glutinosa extract, 90 to 110 parts by weight of forsythia extract, 90 to 110 parts by weight of gardenia extract, 90 to 110 parts by weight of rhizome of scutellaria baicalensis extract, 50 to 70 parts by weight of scutellaria baicalensis extract, 50 to 70 parts by weight of coptis extract, 50 to 70 parts by weight of phellodendron amurense extract and 310 to 330 parts by weight of rehmannia glutinosa extract, and preferably, 95 to 105 parts by weight of raw rehmannia glutinosa extract, 95 to 105 parts by weight of forsythia extract, 95 to 105 parts by weight of gardenia extract and 95 to 105 parts by weight of rhizome of scutellaria baicalensis extract, with respect to 100 parts by weight of honeysuckle extract. It may include, but is not limited to, 55 to 65 parts by weight of golden extract, 55 to 65 parts by weight of coptis extract, 55 to 65 parts by weight of Phellodendron amurense extract, and 315 to 325 parts by weight of Rehmannia glutinosa extract.

[0042] According to one embodiment of the present invention, the composition may include extracts extracted from, but is not limited to, turmeric, astragalus membranaceus, walnut, angelica, astragalus root, turmeric, bilberry, fennel, and rehmannia glutinosa.

[0043] In the present invention, when the composition includes extracts extracted from chrysanthemum, golsaebom, walnut, angelica, astragalus, turmeric, valerian root, fennel and rehmannia root, it may include 375 to 395 parts by weight of golsaebom extract, 190 to 210 parts by weight of walnut extract, 190 to 210 parts by weight of celery, 190 to 210 parts by weight of angelica extract, 190 to 210 parts by weight of astragalus extract, 90 to 110 parts by weight of turmeric extract, 90 to 110 parts by weight of valerian root extract, 90 to 110 parts by weight of fennel extract and 950 to 970 parts by weight of rehmannia root extract, based on 100 parts by weight of the chrysanthemum extract, and preferably, 380 to 390 parts by weight of golsaebom extract, 195 to 205 parts by weight of walnut extract It may include, but is not limited to, 195 to 205 parts by weight of a ginseng extract, 195 to 205 parts by weight of an angelica root extract, 195 to 205 parts by weight of an astragalus membranaceus extract, 95 to 105 parts by weight of a turmeric extract, 95 to 105 parts by weight of a valerian root extract, 95 to 105 parts by weight of a fennel extract, and 955 to 965 parts by weight of a rehmannia root extract.

[0044] In the present invention, “promoting hair growth” means promoting hair growth and / or regeneration of an individual.

[0045] According to an embodiment of the present invention, the hair growth promotion effect can be achieved through activation of follicular stem cells.

[0046] In the present invention, the "follicular stem cells" refer to pluripotent cells located within the hair follicle (bulge region) that play an important role in generating new hair and regenerating damaged tissue during the hair growth cycle.

[0047] The above hair follicle stem cells possess self-renewal capacity and the ability to differentiate and develop into various cell types of the hair follicle. The hair follicle stem cells are activated during the hair growth phase (anagen) and remain dormant during the resting phase (telogen) and catagen phases. The hair follicle stem cells play a key role in the structural and functional maintenance of the hair follicle, and effectively promote hair growth and regeneration through interactions with outer root sheath cells and dermal papilla cells.

[0048] In this respect, the composition of the present invention may contribute to promoting hair production and growth during the hair growth phase by optimizing the microenvironment of hair follicle stem cells, thereby inducing activation of hair follicle stem cells and enhancing signal transmission with hair follicle cells.

[0049] In the present invention, hair growth and / or regeneration can be achieved through a process in which hair is regenerated by preventing hair from falling out easily and promoting the formation of hair follicles in the anagen stage, which is the initial stage of hair growth, thereby inducing the formation of new hair follicles.

[0050] In the experimental examples described below, a C57BL / 6N mouse model, a melanoma frequently used in animal experiments related to hair loss and hair growth, was used, and hair was completely removed. After the composition of the present invention was injected, the degree of hair growth and the degree of division of hair follicle stem cells were observed using an optical microscope. As a result, it was specifically confirmed that the composition of the present invention can induce the migration and division of hair follicle stem cells toward the hair papilla and dermal papilla. This confirms that the composition of the present invention can be used for hair growth promotion.

[0051] In the present invention, the composition may be a preparation for oral or parenteral administration, but is not limited thereto.

[0052] In the present invention, when the composition is a preparation for oral administration, it may further include, but is not limited to, a binder, a lubricant, a disintegrant, an absorption promoter, a preservative, a stabilizer, a fast-acting or sustained-release regulator, etc.

[0053] If the above composition is a preparation for oral administration, it may be formulated as a tablet, capsule, sustained-release tablet, powder, liquid form, oral solution, suspension, etc., but is not limited thereto.

[0054] In the present invention, when the composition is a preparation for parenteral administration, the active substance may be administered by any device capable of moving to a target tissue or cell, and the composition may be formulated and administered as an injection.

[0055] In the present invention, “injection” means injecting a drug solution intradermally, subcutaneously, intramuscularly, intravenously, intraarterially, etc. using a syringe.

[0056] According to an embodiment of the present invention, when the composition of the present invention is formulated as an injection, the injection may be a subcutaneous injection, but is not limited thereto.

[0057] The above syringe may be used without limitation as long as it is a syringe used to administer the composition of the present invention subcutaneously, as well as a general syringe capable of administration through a needle.

[0058] In the present invention, when the composition is formulated (administered) as an injection, it may be in the form of a liquid or dry powder.

[0059] In the present invention, when the composition is administered as an injection, it may be an aqueous injection, a non-aqueous injection, an aqueous suspension injection, a non-aqueous suspension injection, or a solid injection that is dissolved or suspended and used, but is not limited thereto.

[0060] The above injection may further contain distilled water for injection and may further contain a stabilizer or preservative, but is not limited thereto.

[0061] In the present invention, the injection may be included in an amount of 0.1 to 99 wt%, preferably 10 to 99 wt%, of the total weight of the injection, but is not limited thereto.

[0062] In the present invention, “administration” means providing a predetermined composition of the present invention to a subject by any appropriate method.

[0063] In the present invention, the term "subject" means a subject requiring treatment for a disease, and more specifically, a mammal such as a human or non-human primate, mouse, rat, dog, cat, horse, and cow.

[0064] The dosage of the composition of the present invention can be appropriately selected by a skilled technician according to the patient's condition and weight, type and degree of disease, administration route and period.

[0065] Additionally, the above administration may be administered once a day or divided into several times.

[0066] Specifically, the composition of the present invention may be included in an amount of 0.001 to 50 (w / v)%, preferably 0.001 to 40 (w / v)%, 0.001 to 30 (w / v)%, 0.001 to 25 (w / v)%, 0.1 to 25 (w / v)%, 1 to 25 (w / v)%, 10 to 25 (w / v)%, based on the total volume of the composition, but is not limited thereto.

[0067] According to an embodiment of the present invention, the composition of the present invention may be included in an amount of 15 to 20 (w / v)% relative to the total volume of the composition, but is not limited thereto.

[0068] If the above composition is included in less than 0.001 (w / v)%, the hair growth promotion effect may be minimal, and if it is included in more than 50 (w / v)%, side effects may occur in the body.

[0069] The composition of the present invention can be administered topically to a hair loss area or an area where hair growth is desired to be promoted, preferably to the dermis or subcutaneous tissue. The dermis is the thickest layer beneath the epidermis, and is a complex network of blood vessels, nervous systems, lymphatic systems, and other systems. It is the layer where hair follicles develop and grow, and contains hair roots.

[0070] The subcutaneous tissue, located beneath the dermis, between the muscles and bones, contains a large amount of fat cells, which provide softness and contour to the body and can be used as energy. It also represents the circulating arteries and lymphatic fluid.

[0071] The hair growth promoting composition of the present invention may be a pharmaceutical composition, a cosmetic composition, or a composition for external use in the skin.

[0072] The composition of the present invention may further include pharmaceutically acceptable additives within a range that does not impair the physical properties thereof. The additives may include pharmaceutically acceptable carriers, excipients or diluents, preservatives, stabilizers, preservatives, etc. For example, the additives may include, but are not limited to, lactose, dextrose, trehalose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium carbonate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methyl hydroxybenzoate, propyl hydroxybenzoate, registered patent 10-2209294 - 7 - talc, magnesium stearate, mineral oil, etc.

[0073] The content and type of the above additives can be appropriately selected by a person skilled in the art within a range known to those skilled in the art, as long as it does not affect the stability or effectiveness of the active ingredient.

[0074] In the present invention, when the composition is manufactured as a skin external preparation and / or cosmetic composition, it may further include ingredients used in conventional cosmetics or skin external preparations, as long as the effects of the present invention are not impaired.

[0075] For example, it may include, but is not limited to, moisturizers, antioxidants, oil-based ingredients, ultraviolet absorbers, emulsifiers, surfactants, thickeners, alcohols, powdered ingredients, coloring agents, aqueous ingredients, water, and various skin nutrients.

[0076] The external skin preparation and / or cosmetic composition of the present invention may be formulated in various forms. For example, the composition may be, but is not limited to, a shampoo, soap, rinse, surfactant-containing cleanser, cream, lotion, ointment, tonic, treatment, conditioner, suspension, emulsion, paste, gel, oil, wax, spray, aerosol, mist, powder, etc.

[0077] The composition of the present invention can be used alone or in combination with methods using hormone therapy, chemical therapy, and biological response modifiers to promote hair growth.

[0078]

[0079] Another aspect of the present invention is:

[0080] A method for producing a hair growth promoting composition comprising at least one selected from the group consisting of forsythia, rehmannia glutinosa, lotus root, gardenia, pogonyeong, goldenrod, coptis, phellodendron amurense, clove, rhizome, rhizome, golsaebo, walnut, seonmo, angelica, astragalus, turmeric, valerian root, fennel and rehmannia glutinosa is provided.

[0081] Another aspect of the present invention is,

[0082] A step for preparing a hair growth promoting composition comprising at least one selected from the group consisting of forsythia, raw rehmannia root, lotus root, gardenia, pogonyeong, goldenrod, coptis, phellodendron amurense, clove root, chrysanthemum, golsaebo, walnut meat, seonmo, angelica, astragali, turmeric, valerian root, fennel and rehmannia glutinosa; and

[0083] A method for promoting hair growth is provided, comprising a step of administering the composition to an individual.

[0084] In this specification, specific details related to the method for manufacturing the composition and the method for promoting hair growth are substantially the same as the composition unless otherwise stated and are omitted to avoid repetition.

[0085] The composition of the present invention has an effect of promoting hair growth or preventing, improving or treating hair loss in an individual by activating hair follicle stem cells.

[0086] In addition, since the composition of the present invention is composed of herbal medicine extracts, it can minimize the side effects of existing chemical treatments and promote hair growth or prevent, improve or treat hair loss more safely and effectively.

[0087] The effects that can be obtained from the examples are not limited to the effects mentioned above, and other effects that are not mentioned can be clearly derived and understood by a person having ordinary skill in the art based on the detailed description below.

[0088] The accompanying drawings, which are included as part of the detailed description to aid understanding of the embodiments, provide various embodiments and, together with the detailed description, describe technical features of the various embodiments.

[0089] Figure 1 shows the results of visual observation of the example and control groups on the 13th day.

[0090] Figure 2 shows the results of visual observation of the example and control groups administered on the 16th day.

[0091] Figure 3 shows the results of H&E staining observations on days 1, 3, and 5.

[0092] Figure 4 shows the results of H&E staining observations on days 7, 9, and 12.

[0093] Figure 5 shows the results of Toluidine blue staining observation on days 1, 3, and 5.

[0094] Figure 6 shows the results of Toluidine blue staining observation on days 7, 9, and 12.

[0095] Hereinafter, the present invention will be described in more detail through examples. However, these examples are intended to exemplify the present invention, and the scope of the present invention is not limited by these examples. In addition, each component or feature may be considered optional unless otherwise explicitly stated. Each component or feature may be implemented in a form that is not combined with other components or features. Furthermore, various embodiments may be configured by combining some components and / or features. Some components or features of one embodiment may be included in another embodiment or may be replaced with corresponding components or features of another embodiment. Furthermore, terms not specifically defined herein should be understood to have the meaning commonly used in the technical field to which the present invention pertains.

[0096]

[0097] Example 1. Preparation of LMW19-A

[0098] The sample of Example 1 was prepared by mixing extracts of forsythia, rehmannia glutinosa, lotus root, gardenia, pogonyeong, goldenrod, coptis and phellodendron (manufacturer: AJ Tangjeonwon) and extract of rehmannia glutinosa (manufacturer: AJ Tangjeonwon) in a volume ratio of 1:1.

[0099] The composition of the sample of Example 1 above is as follows.

[0100] Medicinal ingredient name Content (g / ml) Honeysuckle 0.039 Raw Rehmannia glutinosa 0.039 Forsythia 0.039 Gardenia jasminoides 0.039 Poria cocos 0.03 Scutellaria baicalensis 0.024 Coptis chinensis 0.024 Phellodendron amurense 0.024 Rehmannia glutinosa 0.125

[0101]

[0102] Example 2. Preparation of LMW19-B

[0103] A mixed extract of total white, bone, walnut, scutellaria baicalensis, angelica, astragalus, turmeric, gilt root, and fennel (manufacturer: AJ Tangjeonwon) and an extract of rehmannia glutinosa (manufacturer: AJ Tangjeonwon) were mixed in a volume ratio of 1:1 to prepare a sample of Example 2.

[0104] The composition of the sample of Example 2 above is as follows.

[0105] Medicinal Herb Name Content (g / ml) Total White 0.013 Bone Grain 0.0 Walnut Meat 0.025 Seonmo 0.025 Angelica 0.025 Astragali 0.025 Turmeric 0.013 Gall Root 0.013 Fennel 0.013 Rehmannia Root 0.125

[0106]

[0107] Experimental example

[0108] (1) Experimental animals

[0109] The animals used in the experiment were C57BL / 6N, a melanoma mouse model widely used in animal experiments related to hair loss and hair growth. A total of 42 6-week-old male mice weighing approximately 25 g were used, and were divided into 39 experimental mice and 3 control mice (normal mice without hair removal).

[0110] The above species is a species that allows for visual observation of the hair growth effect, and has the characteristic of developing circular hair loss of about 5-10 mm in diameter on the back in females after 2 years, so it is widely used in hair loss and hair growth research.

[0111]

[0112] (2) Experimental method

[0113] At least 1-2 mice from each experimental group were sacrificed on the 1st, 3rd, 5th, 7th, 9th, and 12th days after hair removal to prepare specimens for observing optical microscopic tissue findings. On the 13th and 16th days, when hair growth began and visual comparison was possible, camera photography was performed to observe the visual hair growth effect and make comparisons.

[0114] The first day of the experiment was the day when the hair was completely removed with hair removal cream (Veet RaseraTM, Korea) after shaving with animal clippers.

[0115] Meanwhile, to examine the characteristics related to hair growth occurring in the skin, changes such as the movement of stem cells in the skin were observed using an optical microscope.

[0116] Specifically, in order to histologically observe changes such as migration of stem cells in the skin, saline was injected into the control group, and 100 ㎕ of the samples of Example 1 and Example 2 were injected into the dorsal skin once a day into the experimental group, and 1-2 mice were sacrificed on each day (1, 3, 5, 7, 9, and 12 days), and the dorsal skin was incised parallel to the spinal line to excise some skin tissue and fix it with normal buffered formalin solution.

[0117] After fixation, the specimens were washed and dehydrated using the usual method and embedded in paraffin or JB4 (epoxy resin). 2 μm sections were made using an ultra microtome (Leica RM2165, Germany) for the specimens embedded in JB4 (epoxy resin) after embedding, and 7 μm sections were made using a rotary microtome (Jung Hist°ut 820, Germany) for the specimens embedded in paraffin, and toluidine blue staining and H&E staining were performed, respectively.

[0118]

[0119] (3) Results

[0120] The results of visual observation are shown in Figures 1 and 2, and the experimental groups administered with the samples of Examples 1 and 2 all showed an accelerated hair growth period compared to the control group, confirming that the composition of the present invention has a hair growth promotion effect.

[0121] In addition, with regard to optical microscopic observation after H&E and Toluidine Blue staining, the H&E staining observation results are shown in Figs. 3 and 4, and the Toluidine Blue staining results are shown in Figs. 5 and 6.

[0122] In the H&E staining findings, it was observed that the group administered with the sample of the example had more stem cells dividing toward the hair papilla and dermal papilla compared to the control group.

[0123] In addition, in the Toluidine Blue staining findings, in the administration group of the example, stem cells were dividing and moving toward the hair papilla and dermal papilla under the plucked hair follicles stained with Toluidine Blue, and in particular, many dividing cells strongly stained with Toluidine Blue were observed at the base of the hair follicle. In the control group, staining with Toluidine Blue was weaker than in the experimental group, and dividing cells were not observed as much as in the experimental group.

[0124] As a result, it was confirmed that the composition of the present invention has a hair growth promoting effect by inducing division of hair follicle stem cells and promoting their migration to the hair papilla and dermal papilla.

[0125]

[0126] The various embodiments described above may be embodied in other specific forms without departing from the technical idea and essential characteristics thereof. Therefore, the above detailed description should not be construed as limiting in all respects but rather as illustrative. The scope of the various embodiments should be determined by a reasonable interpretation of the appended claims, and all changes within the equivalent scope of the various embodiments are included within the scope of the various embodiments. Furthermore, claims that are not explicitly cited in the claims may be combined to form an embodiment or incorporated into a new claim through a post-application amendment.

Claims

1. A composition for promoting hair growth, comprising an extract extracted from at least one selected from the group consisting of forsythia, rehmannia glutinosa, lotus root, gardenia, pogonyeong, goldenrod, coptis, phellodendron amurense, clove root, chrysanthemum, golsaebo, walnut, seonmo, angelica, astragalus, turmeric, valerian root, fennel and rehmannia glutinosa.

2. In paragraph 1, The composition for promoting hair growth comprises extracts extracted from forsythia, rehmannia glutinosa, lotus root, gardenia, pogonyeong, goldenrod, coptis, phellodendron amurense and rehmannia glutinosa.

3. In paragraph 2, A composition for promoting hair growth, comprising 90 to 110 parts by weight of raw Rehmannia glutinosa extract, 90 to 110 parts by weight of forsythia extract, 90 to 110 parts by weight of gardenia extract, 90 to 110 parts by weight of Pogonyeong extract, 50 to 70 parts by weight of Scutellaria baicalensis extract, 50 to 70 parts by weight of Coptis chinensis extract, 50 to 70 parts by weight of Phellodendron amurense extract, and 310 to 330 parts by weight of Rehmannia glutinosa extract, relative to 100 parts by weight of the above-mentioned forsythia extract.

4. In paragraph 1, A composition for promoting hair growth, the composition comprising extracts extracted from ginseng, ginseng root, walnut, angelica, astragalus, turmeric, valerian root, fennel and cinnamon.

5. In paragraph 4, A composition for promoting hair growth, comprising, with respect to 100 parts by weight of the above-mentioned total extract, 375 to 395 parts by weight of a bone extract, 190 to 210 parts by weight of a walnut extract, 190 to 210 parts by weight of a scutellaria root extract, 190 to 210 parts by weight of an angelica root extract, 190 to 210 parts by weight of an astragalus root extract, 90 to 110 parts by weight of a turmeric extract, 90 to 110 parts by weight of a gallic root extract, 90 to 110 parts by weight of a fennel extract, and 950 to 970 parts by weight of a rehmannia root extract.

6. In paragraph 1, A composition for promoting hair growth, wherein the above-mentioned promotion of hair growth is due to the activation of follicular stem cells.

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